Rh6AG294200
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0015880
Physical Location & Seq
Forward (+)
49106576 .. 49109756
3181 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG294200.1

Sequence Viewer

Length: 3039 bp
ATGAAGATTTTGCTCATCTTCTTCTTCTTTTACTGTTCCATTGGTTTTGCTTCTTATGGTTCTGCTGCTGTGGCTAACAATGATGAAGCATCTGCCTTGCTTTCGCTAAAAGCAGGTCTGCTTGATCCTTTGGAGAGTCTTAAGGATTGGAAAGTCCAATCTGATCACTGTAACTGGACTGGTGTATGGTGCAATAGTCAGGGTCACGTCGAGAAGCTTGATCTCTCCCACATGAATCTCAGTGGTTCAGTCTCAGGTGACATCCAATGGCTCAAAAGTCTCACTAGTCTCAACTTGTGCTGCAGTGCATTCTCTTCATCATTGCCGAAATCCATGTCGAATCTCACAGCACTGAAGAGCCTTGATGTCAGCCAGAATTCCTTTGTTGGTGATTTTCCAGTGGGACTTGGAAGGGCGGATGGCTTGTCATATCTGAATGCTTCCAGCAACAACTTCTCGGGTTTTCTGCCCGAGGATCTTGGCAATGCCACAATGCTCGAGACTCTGGATCTTCGAGGGAGCTTCTTTGAAGGGGAGATTCCGAAGTCCTTCAGGAAGTTGCAGAAGCTGAAGTTTCTTGGCCTCTCTGGGAATAACCTCACAGGTCAAATCCCATCAGAGCTTGGGAAACTGTTGTCATTGGAGAGCATCATTCTTGGGTATAATGAGTTTCAAGGTGGGATTCCATTGGAATTTGGAAATCTCACCAACTTGAAGTATCTTGATTTGGCAGTTGGCAATCTTAGTGGTGAGATTCCAGCTGAGTTGGGGAAGCTGAAGCAACTAGAGACAGTGTTCTTGTACAAGAACAGCTTTGAAGGTAGAATCCCACCTCAAATCAGCACCATTCCTTCGTTGCAGTTTCTTGACCTATCCGATAACAAGTTATCGGGTGAAATCCCATCAGAGATAGGTGAGCTGGAGAACTTGCAGCTTCTGAATCTCATGTGCAATAAGTTGTCCGGTTCAGTACCATTGGGACTTGGAAACTTGACTCAGTTAGTGGTTCTTGAGCTGTGGAACAATTCTTTATCAGGCTCTTTACCAAGTGATCTTGGTAAAAATGCTCCATTGTTGTGGTTGGATATATCGTCCAACTCATTCTCCGGTGAGATTCCAGCGACATTGTGCAATAAAGGCAATCTCACCAAGCTCATCCTCTTCAACAATGCCTTCACAGGTCCAATTCCAGTTGGCTTGTCAACCTGTGTTTCACTTGTTCGTGTCCGAATGCAGAACAATCTTCTTTCAGGGACAATCCCAGTTGGTCTTGGCAAGCTTGGGAGGCTTCAAAGGTTAGAATTGGCTAATAACAATCTCACTGGTGCAATCCCAGATGACATTTCTTCTTCTACTTCAATTTCTTTCATTGATCTCTCTCGAAACCACCTCCATTCTTCCGTTCCTTCAACAATTCTCTCTGCTCCAAGTTTGCAAACCTTCATGGCCTCGAATAATTTCTTAGTAGGAGAGATTCCAGACCAGTTCCAGGACTGTCCTTCACTTTCTGTGCTTGATCTCTCATCAAACAGTTTCTCTGGAACCATTCCAGCAAGCATTGGTTCATGTGAGAGATTGGTGAATTTGAATCTTGCCAACAACCAATTAACTGGTGAAATCCCCAAGTCAATTGCCATGATGCCCACATTGTCCATTTTGGACTTGTCCAACAACTCTCTCACTGGTGGAATACCAGAAAATTTTGGAATCTCTCCAGCCTTGGAAACTCTCAATGTCTCATACAACAAGCTAGAGGGTGCTATCCCAGCAAATGGTGTGCTTAGAACAATAAACCCAAGTGATCTTGTAGGCAATGCTGGTCTCTGCGGTGGTGTCCTCCCTCCTTGTGCCCGAAATCCAGCATTGGAATCGAGGCATAGGAGCTTGCACACTAAGAATATTATTGCAGGATGGGTGATTGGCATTTCATCAGCTTTAGCAGTTGGAATTGCACTTCTTGGTGCAAGGTCTCTATACAAGAGGTGGTATTCGAATGGAAGTTGCTTCGACGATAGATTCGATGTGGGAAATGGAGAATGGCCATGGAGATTAATGGCATTCCAGAGGCTTGGTTTCACTAGTGCTGACATTTTGGCTTGTGTGAAGGAATCAAATGTGATTGGAATGGGAGCTACTGGCGTTGTCTATAAGGCTGAGATAACAAGATCAAACACAGTTGTTGCAGTTAAGAAGTTGTGGAGACCAGCAACAGATGTTGAAACTGGAAGCAGTGGTGATCTAGTGGGGGAAGTGAATCTCTTGGGGAAGCTAAGGCATCGAAACATTGTTCGGTTACTAGGATTTCTGAACAATGATACCAATTTGATGATTATATATGAGTTTATGAGCAATGGCAGCCTTGGAGAAGCCCTTCATGGCAAGCAAGCTGCGAGGTTGCTTGTAGATTGGGTTACAAGGTATAACATAGTAGTCGGAGTTGCGCAAGGTCTTGCTTATCTCCACCATGATTGTCACCCACCAGTCATTCACAGAGACATCAAGTCCAATAACATACTACTCGATGCAAACCTTGAGGCTAGGATTGCAGATTTCGGGTTGGCAAGGATGATGGTCCGAAAAAATGAGACAGTTTCCATGGTGGCTGGTTCATACGGCTACATTGCCCCTGAATATGGATACACATTGAAGGTTGATGAGAAAATTGACATCTACAGCTATGGTGTGGTTCTATTGGAGCTTCTAACAGGAAAGAGGCCATTAGACCCTGAGTTTGGAGAGTCGGTAGACATCGTGGAATGGATTCGAAGGAAGATTAGGGACAACAAATGTTTAGAAGAAGTATTAGACCCCAGTGTAGGAAATTGCAAGCATGTTCAAGAAGAGATGCTCTTAGTCCTTAGAATAGCACTTGTTTGCACTACCAAGCTTCCCAAGGACAGACCCTCCATGAGAGATGTCATAACCATGCTTGGAGAGGCTAAGCCTCGACGGAAAAGCAGTAGCAACAACAATGATGCATATGCAACTAACAAAGACCAGCCAGTCTTTAGTACCTCACCTGTAAATGGTCTTCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

1012

Amino Acids

109.72

Weight (kDa)

5.89

Isoelectric Point (pI)

31.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 26 - 65 4.7e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 142 - 217 7.5e-07 Leucine-rich repeat region
LRR_14 PF23598 232 - 340 9.8e-12 Leucine-rich repeat region
LRR_14 PF23598 327 - 558 1.6e-07 Leucine-rich repeat region
LRR_8 PF13855 476 - 536 3.1e-07 Leucine rich repeat
LRR_8 PF13855 525 - 584 5.8e-07 Leucine rich repeat
Pkinase PF00069 703 - 974 1.2e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 703 - 976 5.2e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015880)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28650
fragaria_vesca FvH4_2g20490
malus_domestica MD05G1188700.v1.1 MD10G1176300.v1.1
prunus_persica Prupe.8G215300_v2.0.a1
pyrus_communis pycom10g15220
rosa_chinensis RchiOBHm_Chr6g0286591
rosa_laevigata RLG00000012556
rosa_multiflora Rmu_sc0000560.1_g000003
rosa_roxburghii Rroxscaffold_7G00182160
rosa_rugosa Rorug06G0181300
rosa_samantha Rh6AG294200 Rh6BG297300 Rh6CG297700 Rh6DG289600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 3002
Acc16I TGCGCA 1 cut(s) 2442
Acc36I ACCTGC 1 cut(s) 104
AccB7I CCANNNNNTGG 1 cut(s) 1772
AccI GTMKAC 1 cut(s) 2745
AciI CCGC 2 cut(s) 416, 1827
AclWI GGATC 3 cut(s) 119, 483, 516
AcoI YGGCCR 1 cut(s) 2039
AcsI RAATTY 4 cut(s) 376, 692, 1582, 1699
AcuI CTGAAG 4 cut(s) 374, 535, 590, 797
AfaI GTAC 3 cut(s) 803, 972, 3013
AfiI CCNNNNNNNGG 6 cut(s) 1489, 1772, 2633, 2732, 2816, 3026
AflII CTTAAG 1 cut(s) 140
AhdI GACNNNNNGTC 1 cut(s) 2500
AhlI ACTAGT 2 cut(s) 284, 2078
AjiI CACGTC 1 cut(s) 208
AjnI CCWGG 1 cut(s) 1488
AlwI GGATC 3 cut(s) 119, 483, 516
AlwNI CAGNNNCTG 2 cut(s) 568, 937
Ama87I CYCGRG 3 cut(s) 457, 470, 497
AoxI GGCC 4 cut(s) 580, 1446, 2039, 2714
ApeKI GCWGC 5 cut(s) 65, 300, 931, 2355, 2387
ApoI RAATTY 4 cut(s) 376, 692, 1582, 1699
ArsI GACNNNNNNTTYG 6 cut(s) 320, 352, 2000, 2032, 2714, 2746
AseI ATTAAT 1 cut(s) 2051
Asp700I GAANNNNTTC 4 cut(s) 548, 1457, 2370, 2760
AspLEI GCGC 1 cut(s) 2443
AspS9I GGNCC 2 cut(s) 1181, 2572
AsuII TTCGAA 2 cut(s) 1991, 2764
AvaI CYCGRG 3 cut(s) 457, 470, 497
AvaII GGWCC 2 cut(s) 1181, 2572
BaeGI GKGCMC 1 cut(s) 1852
BalI TGGCCA 1 cut(s) 2041
BarI GAAGNNNNNNTAC 1 cut(s) 3015
BbsI GAAGAC 1 cut(s) 3023
BbvI GCAGC 5 cut(s) 52, 287, 943, 2367, 2374
BccI CCATC 5 cut(s) 413, 622, 910, 1905, 2563
BceAI ACGGC 1 cut(s) 2629
BcgI CGANNNNNNTGC 2 cut(s) 1851, 1885
BciT130I CCWGG 1 cut(s) 1490
BciVI GTATCC 1 cut(s) 2630
BclI TGATCA 1 cut(s) 163
BcuI ACTAGT 2 cut(s) 284, 2078
BfaI CTAG 7 cut(s) 285, 785, 1751, 2079, 2240, 2297, 2538
BfmI CTRYAG 3 cut(s) 301, 2671, 3035
BfrI CTTAAG 1 cut(s) 140
BfuAI ACCTGC 1 cut(s) 104
BfuI GTATCC 1 cut(s) 2630
BisI GCNGC 5 cut(s) 66, 301, 932, 2356, 2388
BlpI GCTNAGC 1 cut(s) 2940
BlsI GCNGC 5 cut(s) 67, 302, 933, 2357, 2389
Bme1390I CCNGG 1 cut(s) 1490
Bme18I GGWCC 2 cut(s) 1181, 2572
BmeRI GACNNNNNGTC 1 cut(s) 2500
BmeT110I CYCGRG 3 cut(s) 457, 470, 497
BmgBI CACGTC 1 cut(s) 208
BmgT120I GGNCC 2 cut(s) 1181, 2572
BmiI GGNNCC 1 cut(s) 1543
BmrFI CCNGG 1 cut(s) 1490
BmrI ACTGGG 2 cut(s) 1256, 2805
BmsI GCATC 7 cut(s) 98, 657, 1629, 2284, 2512, 2835, 2965
BmuI ACTGGG 2 cut(s) 1256, 2805
BpiI GAAGAC 1 cut(s) 3023
BpmI CTGGAG 2 cut(s) 941, 1698
Bpu10I CCTNAGC 1 cut(s) 2270
Bpu1102I GCTNAGC 1 cut(s) 2940
Bpu14I TTCGAA 2 cut(s) 1991, 2764
BpuEI CTTGAG 2 cut(s) 1031, 2552
BsaI GGTCTC 3 cut(s) 1826, 1974, 2194
BsaJI CCNNGG 6 cut(s) 471, 1719, 2042, 2359, 2595, 2892
BsaWI WCCGGW 2 cut(s) 962, 1106
BsaXI ACNNNNNCTCC 6 cut(s) 1088, 1118, 2727, 2757, 2888, 2918
Bsc4I CCNNNNNNNGG 6 cut(s) 1489, 1772, 2633, 2732, 2816, 3026
Bse3DI GCAATG 5 cut(s) 320, 490, 1819, 2356, 2619
BseBI CCWGG 1 cut(s) 1490
BseDI CCNNGG 6 cut(s) 471, 1719, 2042, 2359, 2595, 2892
BseGI GGATG 5 cut(s) 261, 424, 1155, 1916, 2571
BseLI CCNNNNNNNGG 6 cut(s) 1489, 1772, 2633, 2732, 2816, 3026
BseMI GCAATG 5 cut(s) 320, 490, 1819, 2356, 2619
BseMII CTCAG 6 cut(s) 253, 267, 753, 1010, 2145, 2718
BseSI GKGCMC 1 cut(s) 1852
BseXI GCAGC 5 cut(s) 52, 287, 943, 2367, 2374
BseYI CCCAGC 1 cut(s) 1765
BshFI GGCC 4 cut(s) 582, 1448, 2041, 2716
BsiHKCI CYCGRG 3 cut(s) 457, 470, 497
BsiSI CCGG 2 cut(s) 963, 1107
BslFI GGGAC 4 cut(s) 417, 993, 1267, 2792
BslI CCNNNNNNNGG 6 cut(s) 1489, 1772, 2633, 2732, 2816, 3026
BsmFI GGGAC 4 cut(s) 417, 993, 1267, 2792
BsmI GAATGC 4 cut(s) 308, 442, 1236, 2057
BsnI GGCC 4 cut(s) 582, 1448, 2041, 2716
Bso31I GGTCTC 3 cut(s) 1826, 1974, 2194
BsoBI CYCGRG 3 cut(s) 457, 470, 497
Bsp119I TTCGAA 2 cut(s) 1991, 2764
Bsp1286I GDGCHC 1 cut(s) 1852
Bsp1407I TGTACA 1 cut(s) 801
Bsp1720I GCTNAGC 1 cut(s) 2940
Bsp19I CCATGG 2 cut(s) 2042, 2595
BspACI CCGC 2 cut(s) 416, 1827
BspANI GGCC 4 cut(s) 582, 1448, 2041, 2716
BspCNI CTCAG 6 cut(s) 252, 266, 754, 1009, 2146, 2719
BspLI GGNNCC 1 cut(s) 1543
BspMAI CTGCAG 1 cut(s) 305
BspMI ACCTGC 1 cut(s) 104
BspPI GGATC 3 cut(s) 119, 483, 516
BspQI GCTCTTC 1 cut(s) 350
BspT104I TTCGAA 2 cut(s) 1991, 2764
BspTI CTTAAG 1 cut(s) 140
BspTNI GGTCTC 3 cut(s) 1826, 1974, 2194
BsrDI GCAATG 5 cut(s) 320, 490, 1819, 2356, 2619
BsrGI TGTACA 1 cut(s) 801
BssECI CCNNGG 6 cut(s) 471, 1719, 2042, 2359, 2595, 2892
BssT1I CCWWGG 5 cut(s) 1719, 2042, 2359, 2595, 2892
Bst2UI CCWGG 1 cut(s) 1490
Bst4CI ACNGT 8 cut(s) 35, 170, 633, 793, 1496, 1532, 2176, 2590
Bst6I CTCTTC 4 cut(s) 319, 350, 1166, 2835
BstAFI CTTAAG 1 cut(s) 140
BstAUI TGTACA 1 cut(s) 801
BstBI TTCGAA 2 cut(s) 1991, 2764
BstC8I GCNNGC 6 cut(s) 1277, 1555, 1886, 2381, 2385, 2828
BstDSI CCRYGG 2 cut(s) 2042, 2595
BstF5I GGATG 5 cut(s) 261, 424, 1155, 1916, 2571
BstHHI GCGC 1 cut(s) 2443
BstMWI GCNNNNNNNGC 6 cut(s) 71, 1137, 1285, 1766, 2355, 2543
BstNI CCWGG 1 cut(s) 1490
BstNSI RCATGY 1 cut(s) 2834
BstSCI CCNGG 1 cut(s) 1488
BstSFI CTRYAG 3 cut(s) 301, 2671, 3035
BstSLI GKGCMC 1 cut(s) 1852
BstV1I GCAGC 5 cut(s) 52, 287, 943, 2367, 2374
BstV2I GAAGAC 1 cut(s) 3023
BstX2I RGATCY 2 cut(s) 475, 508
BstXI CCANNNNNNTGG 3 cut(s) 1077, 1610, 2069
BstYI RGATCY 2 cut(s) 475, 508
BsuI GTATCC 1 cut(s) 2630
BsuRI GGCC 4 cut(s) 582, 1448, 2041, 2716
BtgI CCRYGG 2 cut(s) 2042, 2595
BtrI CACGTC 1 cut(s) 208
BtsCI GGATG 5 cut(s) 261, 424, 1155, 1916, 2571
BtsI GCAGTG 2 cut(s) 310, 2236
BveI ACCTGC 1 cut(s) 104
Cac8I GCNNGC 6 cut(s) 1277, 1555, 1886, 2381, 2385, 2828
CaiI CAGNNNCTG 2 cut(s) 568, 937
CfoI GCGC 1 cut(s) 2443
Cfr13I GGNCC 2 cut(s) 1181, 2572
Csp6I GTAC 3 cut(s) 802, 971, 3012
CspCI CAANNNNNGTGG 4 cut(s) 727, 762, 2450, 2485
CviQI GTAC 3 cut(s) 802, 971, 3012
DrdI GACNNNNNNGTC 1 cut(s) 3002
DriI GACNNNNNGTC 1 cut(s) 2500
DseDI GACNNNNNNGTC 1 cut(s) 3002
EaeI YGGCCR 1 cut(s) 2039
Eam1104I CTCTTC 4 cut(s) 319, 350, 1166, 2835
Eam1105I GACNNNNNGTC 1 cut(s) 2500
EarI CTCTTC 4 cut(s) 319, 350, 1166, 2835
EciI GGCGGA 1 cut(s) 431
Eco130I CCWWGG 5 cut(s) 1719, 2042, 2359, 2595, 2892
Eco31I GGTCTC 3 cut(s) 1826, 1974, 2194
Eco47I GGWCC 2 cut(s) 1181, 2572
Eco57I CTGAAG 4 cut(s) 374, 535, 590, 797
Eco88I CYCGRG 3 cut(s) 457, 470, 497
EcoRI GAATTC 1 cut(s) 376
EcoRII CCWGG 1 cut(s) 1488
EcoT14I CCWWGG 5 cut(s) 1719, 2042, 2359, 2595, 2892
EcoT22I ATGCAT 1 cut(s) 2980
ErhI CCWWGG 5 cut(s) 1719, 2042, 2359, 2595, 2892
FalI AAGNNNNNCTT 2 cut(s) 797, 829
FaqI GGGAC 4 cut(s) 417, 993, 1267, 2792
FauNDI CATATG 1 cut(s) 2980
FbaI TGATCA 1 cut(s) 163
FblI GTMKAC 1 cut(s) 2745
Fnu4HI GCNGC 5 cut(s) 66, 301, 932, 2356, 2388
FokI GGATG 5 cut(s) 248, 431, 1142, 1923, 2578
Fsp4HI GCNGC 5 cut(s) 66, 301, 932, 2356, 2388
FspBI CTAG 7 cut(s) 285, 785, 1751, 2079, 2240, 2297, 2538
FspI TGCGCA 1 cut(s) 2442
GlaI GCGC 1 cut(s) 2442
GluI GCNGC 5 cut(s) 66, 301, 932, 2356, 2388
GsaI CCCAGC 1 cut(s) 1769
GsuI CTGGAG 2 cut(s) 941, 1698
HaeIII GGCC 4 cut(s) 582, 1448, 2041, 2716
HapII CCGG 2 cut(s) 963, 1107
HhaI GCGC 1 cut(s) 2443
Hin6I GCGC 1 cut(s) 2441
HinP1I GCGC 1 cut(s) 2441
HincII GTYRAC 1 cut(s) 1203
HindII GTYRAC 1 cut(s) 1203
HindIII AAGCTT 3 cut(s) 215, 1277, 2885
HpaII CCGG 2 cut(s) 963, 1107
Hpy166II GTNNAC 2 cut(s) 1203, 2746
Hpy8I GTNNAC 2 cut(s) 1203, 2746
Hpy99I CGWCG 3 cut(s) 212, 2012, 2952
HpyCH4III ACNGT 8 cut(s) 35, 170, 633, 793, 1496, 1532, 2176, 2590
HpyCH4IV ACGT 1 cut(s) 207
HpyF10VI GCNNNNNNNGC 6 cut(s) 71, 1137, 1285, 1766, 2355, 2543
HpySE526I ACGT 1 cut(s) 207
HspAI GCGC 1 cut(s) 2441
Ksp22I TGATCA 1 cut(s) 163
LguI GCTCTTC 1 cut(s) 350
LmnI GCTCC 6 cut(s) 519, 1072, 1429, 1881, 2129, 2695
Lsp1109I GCAGC 5 cut(s) 52, 287, 943, 2367, 2374
LweI GCATC 7 cut(s) 98, 657, 1629, 2284, 2512, 2835, 2965
MaeI CTAG 7 cut(s) 285, 785, 1751, 2079, 2240, 2297, 2538
MaeII ACGT 1 cut(s) 207
MaeIII GTNAC 6 cut(s) 170, 203, 257, 2292, 2410, 2471
MfeI CAATTG 1 cut(s) 1629
MflI RGATCY 2 cut(s) 475, 508
MhlI GDGCHC 1 cut(s) 1852
MlsI TGGCCA 1 cut(s) 2041
MluNI TGGCCA 1 cut(s) 2041
MlyI GAGTC 4 cut(s) 145, 496, 988, 2747
MmeI TCCRAC 5 cut(s) 1062, 1119, 1692, 1924, 2413
Mox20I TGGCCA 1 cut(s) 2041
Mph1103I ATGCAT 1 cut(s) 2980
MroXI GAANNNNTTC 4 cut(s) 548, 1457, 2370, 2760
MscI TGGCCA 1 cut(s) 2041
MseI TTAA 4 cut(s) 141, 1607, 2051, 2187
MslI CAYNNNNRTG 2 cut(s) 1075, 2924
Msp20I TGGCCA 1 cut(s) 2041
MspA1I CMGCKG 1 cut(s) 761
MspCI CTTAAG 1 cut(s) 140
MspI CCGG 2 cut(s) 963, 1107
MspR9I CCNGG 1 cut(s) 1490
MunI CAATTG 1 cut(s) 1629
Mva1269I GAATGC 4 cut(s) 308, 442, 1236, 2057
MvaI CCWGG 1 cut(s) 1490
MwoI GCNNNNNNNGC 6 cut(s) 71, 1137, 1285, 1766, 2355, 2543
NcoI CCATGG 2 cut(s) 2042, 2595
NdeI CATATG 1 cut(s) 2980
NlaIV GGNNCC 1 cut(s) 1543
NmuCI GTSAC 3 cut(s) 203, 257, 2471
NsbI TGCGCA 1 cut(s) 2442
NsiI ATGCAT 1 cut(s) 2980
NspI RCATGY 1 cut(s) 2834
NspV TTCGAA 2 cut(s) 1991, 2764
PaeR7I CTCGAG 1 cut(s) 497
PciSI GCTCTTC 1 cut(s) 350
PcsI WCGNNNNNNNCGW 1 cut(s) 2016
PctI GAATGC 4 cut(s) 308, 442, 1236, 2057
PdmI GAANNNNTTC 4 cut(s) 548, 1457, 2370, 2760
PflMI CCANNNNNTGG 1 cut(s) 1772
PfoI TCCNGGA 1 cut(s) 1488
PkrI GCNGC 5 cut(s) 67, 302, 933, 2357, 2389
PleI GAGTC 4 cut(s) 144, 496, 988, 2746
PpsI GAGTC 4 cut(s) 144, 496, 988, 2746
PshBI ATTAAT 1 cut(s) 2051
Psp6I CCWGG 1 cut(s) 1488
PspFI CCCAGC 1 cut(s) 1765
PspGI CCWGG 1 cut(s) 1488
PspN4I GGNNCC 1 cut(s) 1543
PspPI GGNCC 2 cut(s) 1181, 2572
PstI CTGCAG 1 cut(s) 305
PstNI CAGNNNCTG 2 cut(s) 568, 937
PsuI RGATCY 2 cut(s) 475, 508
PvuII CAGCTG 1 cut(s) 761
RsaI GTAC 3 cut(s) 803, 972, 3013
RsaNI GTAC 3 cut(s) 802, 971, 3012
RseI CAYNNNNRTG 2 cut(s) 1075, 2924
SapI GCTCTTC 1 cut(s) 350
SaqAI TTAA 4 cut(s) 141, 1607, 2051, 2187
SatI GCNGC 5 cut(s) 66, 301, 932, 2356, 2388
Sau96I GGNCC 2 cut(s) 1181, 2572
SchI GAGTC 4 cut(s) 145, 496, 988, 2747
ScrFI CCNGG 1 cut(s) 1490
SduI GDGCHC 1 cut(s) 1852
SfaNI GCATC 7 cut(s) 98, 657, 1629, 2284, 2512, 2835, 2965
SfcI CTRYAG 3 cut(s) 301, 2671, 3035
Sfr274I CTCGAG 1 cut(s) 497
SfuI TTCGAA 2 cut(s) 1991, 2764
SinI GGWCC 2 cut(s) 1181, 2572
SlaI CTCGAG 1 cut(s) 497
SmiMI CAYNNNNRTG 2 cut(s) 1075, 2924
SmlI CTYRAG 4 cut(s) 140, 497, 1010, 2531
SmoI CTYRAG 4 cut(s) 140, 497, 1010, 2531
SpeI ACTAGT 2 cut(s) 284, 2078
SsiI CCGC 2 cut(s) 416, 1827
SspI AATATT 1 cut(s) 1900
SspMI CTAG 7 cut(s) 285, 785, 1751, 2079, 2240, 2297, 2538
StyD4I CCNGG 1 cut(s) 1488
StyI CCWWGG 5 cut(s) 1719, 2042, 2359, 2595, 2892
TaaI ACNGT 8 cut(s) 35, 170, 633, 793, 1496, 1532, 2176, 2590
TaiI ACGT 1 cut(s) 210
TatI WGTACW 1 cut(s) 801
Tru1I TTAA 4 cut(s) 141, 1607, 2051, 2187
Tru9I TTAA 4 cut(s) 141, 1607, 2051, 2187
TseFI GTSAC 3 cut(s) 203, 257, 2471
TseI GCWGC 5 cut(s) 65, 300, 931, 2355, 2387
Tsp45I GTSAC 3 cut(s) 203, 257, 2471
TspGWI ACGGA 2 cut(s) 1390, 2965
Van91I CCANNNNNTGG 1 cut(s) 1772
Vha464I CTTAAG 1 cut(s) 140
VpaK11BI GGWCC 2 cut(s) 1181, 2572
VspI ATTAAT 1 cut(s) 2051
XapI RAATTY 4 cut(s) 376, 692, 1582, 1699
XceI RCATGY 1 cut(s) 2834
XhoI CTCGAG 1 cut(s) 497
XmiI GTMKAC 1 cut(s) 2745
XmnI GAANNNNTTC 4 cut(s) 548, 1457, 2370, 2760
XspI CTAG 7 cut(s) 285, 785, 1751, 2079, 2240, 2297, 2538
Zsp2I ATGCAT 1 cut(s) 2980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.