MD10G1176300.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0015880
Physical Location & Seq
Forward (+)
26866784 .. 26870042
3259 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1176300.v1.1.491

Sequence Viewer

Length: 3042 bp
ATGCAGTTGAGCAAAACCCAAATGCTGATCTTCTTCTTTTTGTGCTGTTCTTCATTTGGATTTGCAGCTGTGGCCAATGATGAAGTTTCCGCTCTGCTTTCGATAAAAGCCGGTCTCATTGATCCACTAAACAGCCTTAAAGACTGGAACTTTCCAGAAAATGAAGCTCACTGCAACTGGACAGGCGTATGGTGCAACACTGAGGAGCACGTAGAGAAACTTGATCTCTCCCACATGAACCTCAGCGGCCCGATATCCGGCGACATCCAACGTCTCAAAAGCCTTACTTCCCTCAACCTTTGCTGCAGTGGCTTCTCCTCATCACTGCCAAAATCCATCTCAAATCTCACAGCATTGAAAACTTTTGATGTGAGTCAAAACTCGCTTGTTGGTGAGTTTCCGTGGGCGTTTGGAAGAGCAGGAGGATTGACAGAACTTAACGCTTCGAGTAACAATTTTTCGGATTTTCTTCCTGAGGATCTCGGCAATGCCACAATGCTGGAGACTCTGGATCTCCGAGGGAACTTTTTCGGGGGCTCGTTTCCAAAGTCATTCAAGAACTTGCAGAAGCTCAAGTTTCTTGGGCTTTCCGGGAACAATTTCACCGGGGAAATCCCCGCTGAGCTTGGAGAGCTGTCCTCATTGGAAAGTATGATTCTTGGGTACAATGAGTTTGAAGGTGGGATTCCAATGGAGTTTGGGAATCTTACCAACCTCAAGTATCTTGATTTGGCAGTTGGGAATCTTAGTGGTGAGATTCCAGCCGACTTGGGGAGGCTGAAGTTGCTGGAGACGGTGTTCTTGTACAAGAACAATTTTGAAGGCAAAATCCCAGCTGAAATCGGCACCATTTCTTCGTTGAAATTGCTTGATCTTTCCGATAATGTATTATCGGGGGAAATCCCAGCTGAAATTGAGGAGCTGAAGAATTTGCAGCTTCTGAATGTGATGTGTAATCAGTTATCAGGTTCGGTTCCATTAGGAATTGGAAGTTTGAGTCAGTTAAGTGTTCTTGAGCTATGGAACAATTCGTTTTCAGGTCCTTTGCCCAGTGATCTTGGCAAAAATGCCCCATTGCAGTGGTTGGACGTCTCATCCAACGCGTTTTCCGGCGAGATTCCATCCACATTATGCAACAAGGGCAATCTCACCAAGCTCATCCTCTTCAACAATGCCTTCACAGGTCCAATTCCGGCGAGCCTATCCACTTGCCCTTCGCTTGTTCGTGTTCGAATGCAGAATAATTTTCTTTCCGGGACAATCCCAGTTGGGCTTGGCAAGCTTGAGAGGCTTCAAAGGTTAGAATTGGCAAACAACAATCTCACTGGTGCAATCCCAGATGATCTTTCTTCCTCTACTTCACTCTCTTTCATTGATATCTCTCGAAACCGCCTCCATTCTTCTCTTCCTTCCACCATTCTCTCTGCTCCAAGCTTGCAAACCCTGATGGCCTCGAATAATGAATTGGTTGGCGAAATCCCGGATCAATTCCAGGACTGCCCTTCACTTTCAGTGCTTGATCTCTCATCAAACCATTTCTCAGGAATCATCCCAGCAAGTATTGCTTCATGTGAGAAATTGGTAAGCTTAAATCTGAGGAACAACCAATTGACCGGAGACATCCCGAAATCAATTGCCATGATGCCCACATTGTCCGTTCTTGATCTGTCCAACAACTCTCTCACTGGTGGAATACCTGAAAATTTCGGAATGTCACCGGCCTTGGAGACTCTCAATGTCTCATTCAACAAGCTAGACGGTCCTGTCCCAAAAAACGGTGTACTGAGAACAATAAACCCGAGTGATCTTGTGGGCAATGCCGGTCTCTGTGGCAGTGTCCTCCCTCCATGTACGCAAAATCCAGCATTGGCATCGAGGCATAGGAATGTGCACACAAGGAACATTGTTGCAGGATGGGTGATTGGGATCTCATCAGTTTTAGCAGTTGGACTTGCACTTTTCAGTGCTCGAACTCTATACAAAAGGTGGTACTCAAATGGGAGTTGCTTTGGAGACAGTTTCGAAGTTGGCAAGGGAGAGTGGCCATGGAGACTGATGGCATTCCAGAGGCTTGGTTTCGCAAGTACTGACATTCTGGCTTGTGTGAAGGAATCCAATGTGATCGGAATGGGAGCTACTGGGGTTGTGTACAAGGCAGAGATATCACGATCAAACACAGTTGTGGCAGTTAAAAAGCTGTGGAGACCGGCAACAGACATTGAAACTGGAAGCAGTGATGATCTAGTTGGGGAAGTGAATCTCTTGGGGAGGCTAAGGCATCGAAACATTGTTCGGTTATTAGGATTTCTGAACAATGATACGAATTTGATGATTATATACGAGTTTATGCCCAATGGAAGCTTAGGAGAAACCTTGCATGGCAAGCAAGCAGGGAGGTTGCTTGTAGATTGGGTTTCAAGGTACAACATAGCAGTGGGGGTTGCACAAGGTCTTTCTTATCTCCACCATGACTGTCACCCACCAGTCATCCATAGAGACATCAAGTCTAATAACATACTGCTCGATGCAAACCTCGACGCGAGGATTGCGGACTTCGGGTTGGCACGGATGATGGTTCGAAAAAACGAGACAGTTTCCATGGTGGCTGGATCATACGGTTACATTGCCCCTGAATATGGATACACATTGAAGGTTGATGAAAAGATCGACATCTACAGCTACGGCATGGTTCTATTGGAGCTTCTAACAGGAAAGAGGCCTTTAGACCCCGAGTTTGGCGAATCAGTAGACGTCGTGGAATGGATTCGGACGAAGATTAGGGACAACAAAAGTTTAGAAGAAGCATTAGACCCCAGTGTAGGAAACTGCAAGCATGTTCAAGAAGAGATGCTCTTAGTCCTTAGAATAGCATTGATTTGCACTGCCAAGCTTCCCAAGGACAGGCCTTCCATGAGGGATGTCATAACGATGCTCGGAGAGGCGAAGCCTCGGCGAAAAAGCAGCAGCAACAACGATCCATATGCTGCAGCTAAAAAAGACCAGCCTGTGTTTAGCACCTCACCTGTAAATGGCCTTCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

1014

Amino Acids

110.09

Weight (kDa)

5.53

Isoelectric Point (pI)

30.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 26 - 66 1.2e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 74 - 172 8e-07 Leucine-rich repeat region
LRR_8 PF13855 94 - 153 8.5e-06 Leucine rich repeat
LRR_8 PF13855 167 - 224 5.9e-07 Leucine rich repeat
LRR_14 PF23598 184 - 266 9e-07 Leucine-rich repeat region
LRR_14 PF23598 232 - 339 4.3e-10 Leucine-rich repeat region
LRR_14 PF23598 322 - 563 4.1e-10 Leucine-rich repeat region
LRR_8 PF13855 405 - 465 8.7e-06 Leucine rich repeat
LRR_8 PF13855 477 - 537 4.4e-10 Leucine rich repeat
LRR_8 PF13855 526 - 585 2.5e-08 Leucine rich repeat
Pkinase PF00069 704 - 975 9e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 704 - 977 3.5e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015880)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28650
fragaria_vesca FvH4_2g20490
malus_domestica MD05G1188700.v1.1 MD10G1176300.v1.1
prunus_persica Prupe.8G215300_v2.0.a1
pyrus_communis pycom10g15220
rosa_chinensis RchiOBHm_Chr6g0286591
rosa_laevigata RLG00000012556
rosa_multiflora Rmu_sc0000560.1_g000003
rosa_roxburghii Rroxscaffold_7G00182160
rosa_rugosa Rorug06G0181300
rosa_samantha Rh6AG294200 Rh6BG297300 Rh6CG297700 Rh6DG289600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1763
AatII GACGTC 2 cut(s) 1092, 2754
AccB1I GGYRCC 1 cut(s) 845
AccBSI CCGCTC 1 cut(s) 92
AccI GTMKAC 1 cut(s) 2748
AccII CGCG 2 cut(s) 1103, 2540
AciI CCGC 5 cut(s) 90, 246, 618, 1390, 2549
AclWI GGATC 7 cut(s) 116, 486, 519, 1491, 1934, 2617, 2969
AcoI YGGCCR 2 cut(s) 72, 2042
AcsI RAATTY 3 cut(s) 928, 1702, 2323
AcuI CTGAAG 2 cut(s) 800, 944
AcyI GRCGYC 2 cut(s) 1089, 2751
AfaI GTAC 8 cut(s) 665, 806, 1782, 1852, 1991, 2086, 2150, 2423
AfiI CCNNNNNNNGG 8 cut(s) 257, 771, 1775, 2636, 2735, 2819, 2901, 3029
AflIII ACRYGT 1 cut(s) 1101
AhdI GACNNNNNGTC 1 cut(s) 2503
AjnI CCWGG 1 cut(s) 1491
AjuI GAANNNNNNNTTGG 2 cut(s) 1448, 1480
AleI CACNNNNGTG 1 cut(s) 2180
AloI GAACNNNNNNTCC 2 cut(s) 782, 814
Alw21I GWGCWC 3 cut(s) 210, 1893, 1969
Alw44I GTGCAC 1 cut(s) 1889
AlwI GGATC 7 cut(s) 116, 486, 519, 1491, 1934, 2617, 2969
AlwNI CAGNNNCTG 1 cut(s) 940
Ama87I CYCGRG 2 cut(s) 1798, 2729
AoxI GGCC 8 cut(s) 72, 247, 1449, 1719, 2042, 2717, 2903, 3031
ApaLI GTGCAC 1 cut(s) 1889
ApeKI GCWGC 7 cut(s) 65, 303, 934, 2961, 2964, 2984, 2987
ApoI RAATTY 3 cut(s) 928, 1702, 2323
ArsI GACNNNNNNTTYG 2 cut(s) 2717, 2749
Asp700I GAANNNNTTC 3 cut(s) 527, 599, 2763
AspS9I GGNCC 4 cut(s) 248, 1040, 1184, 1760
AsuC2I CCSGG 4 cut(s) 592, 607, 1255, 1481
AsuHPI GGTGA 8 cut(s) 404, 595, 764, 1141, 1707, 1930, 2468, 3012
AsuII TTCGAA 3 cut(s) 1231, 2022, 2578
AvaI CYCGRG 2 cut(s) 1798, 2729
AvaII GGWCC 3 cut(s) 1040, 1184, 1760
AxyI CCTNAGG 1 cut(s) 474
BaeGI GKGCMC 1 cut(s) 1893
BalI TGGCCA 2 cut(s) 74, 2044
BanI GGYRCC 1 cut(s) 845
BanII GRGCYC 1 cut(s) 539
BarI GAAGNNNNNNTAC 1 cut(s) 3018
Bbv12I GWGCWC 3 cut(s) 210, 1893, 1969
BbvCI CCTCAGC 1 cut(s) 242
BbvI GCAGC 7 cut(s) 77, 290, 946, 2971, 2973, 2976, 2999
BccI CCATC 6 cut(s) 344, 1129, 1441, 1908, 2050, 2566
BceAI ACGGC 1 cut(s) 2698
BcgI CGANNNNNNTGC 4 cut(s) 1854, 1888, 2963, 2997
BciT130I CCWGG 1 cut(s) 1493
BciVI GTATCC 1 cut(s) 2633
BcnI CCSGG 4 cut(s) 592, 607, 1255, 1481
BfaI CTAG 3 cut(s) 1754, 2243, 3040
BfmI CTRYAG 3 cut(s) 304, 2674, 2985
BfuI GTATCC 1 cut(s) 2633
BisI GCNGC 8 cut(s) 66, 247, 304, 935, 2962, 2965, 2985, 2988
BlpI GCTNAGC 1 cut(s) 621
BlsI GCNGC 8 cut(s) 67, 248, 305, 936, 2963, 2966, 2986, 2989
BmcAI AGTACT 1 cut(s) 2086
Bme1390I CCNGG 5 cut(s) 592, 607, 1255, 1481, 1493
Bme18I GGWCC 3 cut(s) 1040, 1184, 1760
BmeRI GACNNNNNGTC 1 cut(s) 2503
BmeT110I CYCGRG 2 cut(s) 1798, 2729
BmgT120I GGNCC 4 cut(s) 248, 1040, 1184, 1760
BmiI GGNNCC 2 cut(s) 847, 975
BmrFI CCNGG 5 cut(s) 592, 607, 1255, 1481, 1493
BmrI ACTGGG 4 cut(s) 1044, 1259, 2148, 2808
BmsI GCATC 6 cut(s) 1632, 1880, 2287, 2515, 2838, 2919
BmuI ACTGGG 4 cut(s) 1044, 1259, 2148, 2808
BplI GAGNNNNNCTC 2 cut(s) 623, 655
BpmI CTGGAG 2 cut(s) 521, 809
Bpu10I CCTNAGC 3 cut(s) 242, 2273, 2362
Bpu1102I GCTNAGC 1 cut(s) 621
Bpu14I TTCGAA 3 cut(s) 1231, 2022, 2578
BpuEI CTTGAG 4 cut(s) 557, 701, 1034, 1304
BpuMI CCSGG 4 cut(s) 592, 607, 1255, 1481
BsaAI YACGTR 1 cut(s) 211
BsaBI GATNNNNATC 2 cut(s) 1925, 2669
BsaHI GRCGYC 2 cut(s) 1089, 2751
BsaI GGTCTC 3 cut(s) 119, 1829, 2197
BsaJI CCNNGG 8 cut(s) 401, 517, 606, 1722, 2045, 2598, 2895, 2948
BsaWI WCCGGW 1 cut(s) 1613
BsaXI ACNNNNNCTCC 2 cut(s) 782, 812
Bsc4I CCNNNNNNNGG 8 cut(s) 257, 771, 1775, 2636, 2735, 2819, 2901, 3029
Bse118I RCCGGY 4 cut(s) 110, 1717, 1820, 2206
Bse21I CCTNAGG 1 cut(s) 474
Bse3DI GCAATG 4 cut(s) 493, 1073, 1822, 2622
Bse8I GATNNNNATC 2 cut(s) 1925, 2669
BseBI CCWGG 1 cut(s) 1493
BseDI CCNNGG 8 cut(s) 401, 517, 606, 1722, 2045, 2598, 2895, 2948
BseJI GATNNNNATC 2 cut(s) 1925, 2669
BseLI CCNNNNNNNGG 8 cut(s) 257, 771, 1775, 2636, 2735, 2819, 2901, 3029
BseMI GCAATG 4 cut(s) 493, 1073, 1822, 2622
BseMII CTCAG 7 cut(s) 192, 256, 465, 612, 1554, 1586, 1775
BseRI GAGGAG 3 cut(s) 218, 307, 932
BseSI GKGCMC 1 cut(s) 1893
BseXI GCAGC 7 cut(s) 77, 290, 946, 2971, 2973, 2976, 2999
BseYI CCCAGC 3 cut(s) 832, 904, 1552
Bsh1236I CGCG 2 cut(s) 1103, 2540
BshFI GGCC 8 cut(s) 74, 249, 1451, 1721, 2044, 2719, 2905, 3033
BshNI GGYRCC 1 cut(s) 845
BsiHKAI GWGCWC 3 cut(s) 210, 1893, 1969
BsiHKCI CYCGRG 2 cut(s) 1798, 2729
BslFI GGGAC 3 cut(s) 1270, 1751, 2795
BslI CCNNNNNNNGG 8 cut(s) 257, 771, 1775, 2636, 2735, 2819, 2901, 3029
BsmBI CGTCTC 3 cut(s) 278, 785, 1096
BsmFI GGGAC 3 cut(s) 1270, 1751, 2795
BsmI GAATGC 2 cut(s) 1239, 2060
BsnI GGCC 8 cut(s) 74, 249, 1451, 1721, 2044, 2719, 2905, 3033
Bso31I GGTCTC 3 cut(s) 119, 1829, 2197
BsoBI CYCGRG 2 cut(s) 1798, 2729
Bsp119I TTCGAA 3 cut(s) 1231, 2022, 2578
Bsp1286I GDGCHC 4 cut(s) 210, 539, 1893, 1969
Bsp1407I TGTACA 2 cut(s) 804, 2148
Bsp1720I GCTNAGC 1 cut(s) 621
Bsp19I CCATGG 2 cut(s) 2045, 2598
BspACI CCGC 5 cut(s) 90, 246, 618, 1390, 2549
BspANI GGCC 8 cut(s) 74, 249, 1451, 1721, 2044, 2719, 2905, 3033
BspCNI CTCAG 7 cut(s) 193, 255, 466, 613, 1553, 1587, 1776
BspFNI CGCG 2 cut(s) 1103, 2540
BspLI GGNNCC 2 cut(s) 847, 975
BspMAI CTGCAG 2 cut(s) 308, 2989
BspPI GGATC 7 cut(s) 116, 486, 519, 1491, 1934, 2617, 2969
BspQI GCTCTTC 1 cut(s) 409
BspT104I TTCGAA 3 cut(s) 1231, 2022, 2578
BspT107I GGYRCC 1 cut(s) 845
BspTNI GGTCTC 3 cut(s) 119, 1829, 2197
BsrBI CCGCTC 1 cut(s) 92
BsrDI GCAATG 4 cut(s) 493, 1073, 1822, 2622
BsrFI RCCGGY 4 cut(s) 110, 1717, 1820, 2206
BsrGI TGTACA 2 cut(s) 804, 2148
BssAI RCCGGY 4 cut(s) 110, 1717, 1820, 2206
BssECI CCNNGG 8 cut(s) 401, 517, 606, 1722, 2045, 2598, 2895, 2948
BssNI GRCGYC 2 cut(s) 1089, 2751
BssT1I CCWWGG 4 cut(s) 1722, 2045, 2598, 2895
Bst2UI CCWGG 1 cut(s) 1493
Bst4CI ACNGT 8 cut(s) 796, 1760, 1778, 2018, 2179, 2474, 2593, 2618
Bst6I CTCTTC 4 cut(s) 409, 1169, 1410, 2838
BstACI GRCGYC 2 cut(s) 1089, 2751
BstAPI GCANNNNNTGC 1 cut(s) 1562
BstAUI TGTACA 2 cut(s) 804, 2148
BstBAI YACGTR 1 cut(s) 211
BstBI TTCGAA 3 cut(s) 1231, 2022, 2578
BstC8I GCNNGC 6 cut(s) 1198, 1280, 1436, 2384, 2388, 2831
BstDSI CCRYGG 3 cut(s) 401, 2045, 2598
BstFNI CGCG 2 cut(s) 1103, 2540
BstNI CCWGG 1 cut(s) 1493
BstNSI RCATGY 1 cut(s) 2837
BstSCI CCNGG 5 cut(s) 590, 605, 1253, 1479, 1491
BstSFI CTRYAG 3 cut(s) 304, 2674, 2985
BstSLI GKGCMC 1 cut(s) 1893
BstUI CGCG 2 cut(s) 1103, 2540
BstV1I GCAGC 7 cut(s) 77, 290, 946, 2971, 2973, 2976, 2999
BstX2I RGATCY 3 cut(s) 478, 511, 1926
BstXI CCANNNNNNTGG 3 cut(s) 499, 1080, 2072
BstYI RGATCY 3 cut(s) 478, 511, 1926
Bsu36I CCTNAGG 1 cut(s) 474
BsuI GTATCC 1 cut(s) 2633
BsuRI GGCC 8 cut(s) 74, 249, 1451, 1721, 2044, 2719, 2905, 3033
BtgI CCRYGG 3 cut(s) 401, 2045, 2598
BtsI GCAGTG 8 cut(s) 169, 313, 323, 1085, 1840, 2239, 2439, 2880
Cac8I GCNNGC 6 cut(s) 1198, 1280, 1436, 2384, 2388, 2831
CaiI CAGNNNCTG 1 cut(s) 940
Cfr10I RCCGGY 4 cut(s) 110, 1717, 1820, 2206
Cfr13I GGNCC 4 cut(s) 248, 1040, 1184, 1760
CseI GACGC 1 cut(s) 2546
Csp6I GTAC 8 cut(s) 664, 805, 1781, 1851, 1990, 2085, 2149, 2422
CviQI GTAC 8 cut(s) 664, 805, 1781, 1851, 1990, 2085, 2149, 2422
DrdI GACNNNNNNGTC 1 cut(s) 1763
DriI GACNNNNNGTC 1 cut(s) 2503
DseDI GACNNNNNNGTC 1 cut(s) 1763
EaeI YGGCCR 2 cut(s) 72, 2042
Eam1104I CTCTTC 4 cut(s) 409, 1169, 1410, 2838
Eam1105I GACNNNNNGTC 1 cut(s) 2503
EarI CTCTTC 4 cut(s) 409, 1169, 1410, 2838
Eco130I CCWWGG 4 cut(s) 1722, 2045, 2598, 2895
Eco147I AGGCCT 2 cut(s) 2719, 2905
Eco24I GRGCYC 1 cut(s) 539
Eco31I GGTCTC 3 cut(s) 119, 1829, 2197
Eco32I GATATC 3 cut(s) 255, 1378, 2163
Eco47I GGWCC 3 cut(s) 1040, 1184, 1760
Eco57I CTGAAG 2 cut(s) 800, 944
Eco81I CCTNAGG 1 cut(s) 474
Eco88I CYCGRG 2 cut(s) 1798, 2729
EcoO109I RGGNCCY 1 cut(s) 1040
EcoRII CCWGG 1 cut(s) 1491
EcoRV GATATC 3 cut(s) 255, 1378, 2163
EcoT14I CCWWGG 4 cut(s) 1722, 2045, 2598, 2895
EcoT38I GRGCYC 1 cut(s) 539
ErhI CCWWGG 4 cut(s) 1722, 2045, 2598, 2895
Esp3I CGTCTC 3 cut(s) 278, 785, 1096
FalI AAGNNNNNCTT 4 cut(s) 271, 303, 1549, 1581
FaqI GGGAC 3 cut(s) 1270, 1751, 2795
FauI CCCGC 1 cut(s) 625
FauNDI CATATG 1 cut(s) 2980
FblI GTMKAC 1 cut(s) 2748
Fnu4HI GCNGC 8 cut(s) 66, 247, 304, 935, 2962, 2965, 2985, 2988
FriOI GRGCYC 1 cut(s) 539
Fsp4HI GCNGC 8 cut(s) 66, 247, 304, 935, 2962, 2965, 2985, 2988
FspBI CTAG 3 cut(s) 1754, 2243, 3040
GluI GCNGC 8 cut(s) 66, 247, 304, 935, 2962, 2965, 2985, 2988
GsaI CCCAGC 3 cut(s) 836, 908, 1556
GsuI CTGGAG 2 cut(s) 521, 809
HaeIII GGCC 8 cut(s) 74, 249, 1451, 1721, 2044, 2719, 2905, 3033
HgaI GACGC 1 cut(s) 2546
Hin1I GRCGYC 2 cut(s) 1089, 2751
HindIII AAGCTT 5 cut(s) 1280, 1432, 1585, 2359, 2888
HphI GGTGA 8 cut(s) 404, 595, 764, 1141, 1707, 1930, 2468, 3012
Hpy166II GTNNAC 4 cut(s) 1781, 1891, 2149, 2749
Hpy8I GTNNAC 4 cut(s) 1781, 1891, 2149, 2749
Hpy99I CGWCG 2 cut(s) 2540, 2756
HpyAV CCTTC 9 cut(s) 671, 815, 1186, 1224, 1419, 1512, 2101, 2644, 2916
HpyCH4III ACNGT 8 cut(s) 796, 1760, 1778, 2018, 2179, 2474, 2593, 2618
HpyCH4IV ACGT 4 cut(s) 210, 271, 1089, 2751
HpySE526I ACGT 4 cut(s) 210, 271, 1089, 2751
Hsp92I GRCGYC 2 cut(s) 1089, 2751
LguI GCTCTTC 1 cut(s) 409
LmnI GCTCC 5 cut(s) 205, 919, 1432, 2132, 2698
Lsp1109I GCAGC 7 cut(s) 77, 290, 946, 2971, 2973, 2976, 2999
LweI GCATC 6 cut(s) 1632, 1880, 2287, 2515, 2838, 2919
MaeI CTAG 3 cut(s) 1754, 2243, 3040
MaeII ACGT 4 cut(s) 210, 271, 1089, 2751
MaeIII GTNAC 4 cut(s) 449, 1713, 2474, 2618
MbiI CCGCTC 1 cut(s) 92
MfeI CAATTG 2 cut(s) 1607, 1632
MflI RGATCY 3 cut(s) 478, 511, 1926
MhlI GDGCHC 4 cut(s) 210, 539, 1893, 1969
MlsI TGGCCA 2 cut(s) 74, 2044
MluI ACGCGT 1 cut(s) 1101
MluNI TGGCCA 2 cut(s) 74, 2044
MlyI GAGTC 4 cut(s) 382, 499, 1006, 1723
MmeI TCCRAC 5 cut(s) 292, 1065, 1122, 1695, 1927
Mox20I TGGCCA 2 cut(s) 74, 2044
MroXI GAANNNNTTC 3 cut(s) 527, 599, 2763
MscI TGGCCA 2 cut(s) 74, 2044
MseI TTAA 5 cut(s) 138, 438, 1004, 1589, 2190
MslI CAYNNNNRTG 5 cut(s) 1078, 1884, 2180, 2432, 2927
Msp20I TGGCCA 2 cut(s) 74, 2044
MspA1I CMGCKG 5 cut(s) 68, 246, 620, 836, 908
MspR9I CCNGG 5 cut(s) 592, 607, 1255, 1481, 1493
MunI CAATTG 2 cut(s) 1607, 1632
Mva1269I GAATGC 2 cut(s) 1239, 2060
MvaI CCWGG 1 cut(s) 1493
MvnI CGCG 2 cut(s) 1103, 2540
NciI CCSGG 4 cut(s) 592, 607, 1255, 1481
NcoI CCATGG 2 cut(s) 2045, 2598
NdeI CATATG 1 cut(s) 2980
NlaIV GGNNCC 2 cut(s) 847, 975
NmeAIII GCCGAG 2 cut(s) 462, 2929
NmuCI GTSAC 2 cut(s) 1713, 2474
NspI RCATGY 1 cut(s) 2837
NspV TTCGAA 3 cut(s) 1231, 2022, 2578
OliI CACNNNNGTG 1 cut(s) 2180
PceI AGGCCT 2 cut(s) 2719, 2905
PciSI GCTCTTC 1 cut(s) 409
PctI GAATGC 2 cut(s) 1239, 2060
PdmI GAANNNNTTC 3 cut(s) 527, 599, 2763
PfoI TCCNGGA 4 cut(s) 590, 1253, 1479, 1491
PkrI GCNGC 8 cut(s) 67, 248, 305, 936, 2963, 2966, 2986, 2989
PleI GAGTC 4 cut(s) 381, 499, 1005, 1723
PpsI GAGTC 4 cut(s) 381, 499, 1005, 1723
Ppu21I YACGTR 1 cut(s) 211
PpuMI RGGWCCY 1 cut(s) 1040
Psp5II RGGWCCY 1 cut(s) 1040
Psp6I CCWGG 1 cut(s) 1491
PspFI CCCAGC 3 cut(s) 832, 904, 1552
PspGI CCWGG 1 cut(s) 1491
PspN4I GGNNCC 2 cut(s) 847, 975
PspPI GGNCC 4 cut(s) 248, 1040, 1184, 1760
PspPPI RGGWCCY 1 cut(s) 1040
PstI CTGCAG 2 cut(s) 308, 2989
PstNI CAGNNNCTG 1 cut(s) 940
PsuI RGATCY 3 cut(s) 478, 511, 1926
PvuII CAGCTG 3 cut(s) 68, 836, 908
RsaI GTAC 8 cut(s) 665, 806, 1782, 1852, 1991, 2086, 2150, 2423
RsaNI GTAC 8 cut(s) 664, 805, 1781, 1851, 1990, 2085, 2149, 2422
RseI CAYNNNNRTG 5 cut(s) 1078, 1884, 2180, 2432, 2927
SapI GCTCTTC 1 cut(s) 409
SaqAI TTAA 5 cut(s) 138, 438, 1004, 1589, 2190
SatI GCNGC 8 cut(s) 66, 247, 304, 935, 2962, 2965, 2985, 2988
Sau96I GGNCC 4 cut(s) 248, 1040, 1184, 1760
ScaI AGTACT 1 cut(s) 2086
SchI GAGTC 4 cut(s) 382, 499, 1006, 1723
ScrFI CCNGG 5 cut(s) 592, 607, 1255, 1481, 1493
SduI GDGCHC 4 cut(s) 210, 539, 1893, 1969
SfaNI GCATC 6 cut(s) 1632, 1880, 2287, 2515, 2838, 2919
SfcI CTRYAG 3 cut(s) 304, 2674, 2985
SfuI TTCGAA 3 cut(s) 1231, 2022, 2578
SinI GGWCC 3 cut(s) 1040, 1184, 1760
SmiMI CAYNNNNRTG 5 cut(s) 1078, 1884, 2180, 2432, 2927
SmlI CTYRAG 4 cut(s) 572, 716, 1013, 1283
SmoI CTYRAG 4 cut(s) 572, 716, 1013, 1283
SseBI AGGCCT 2 cut(s) 2719, 2905
SsiI CCGC 5 cut(s) 90, 246, 618, 1390, 2549
SspMI CTAG 3 cut(s) 1754, 2243, 3040
StuI AGGCCT 2 cut(s) 2719, 2905
StyD4I CCNGG 5 cut(s) 590, 605, 1253, 1479, 1491
StyI CCWWGG 4 cut(s) 1722, 2045, 2598, 2895
TaaI ACNGT 8 cut(s) 796, 1760, 1778, 2018, 2179, 2474, 2593, 2618
TaiI ACGT 4 cut(s) 213, 274, 1092, 2754
TatI WGTACW 4 cut(s) 804, 1780, 2084, 2148
TauI GCSGC 1 cut(s) 249
Tru1I TTAA 5 cut(s) 138, 438, 1004, 1589, 2190
Tru9I TTAA 5 cut(s) 138, 438, 1004, 1589, 2190
TseFI GTSAC 2 cut(s) 1713, 2474
TseI GCWGC 7 cut(s) 65, 303, 934, 2961, 2964, 2984, 2987
Tsp45I GTSAC 2 cut(s) 1713, 2474
TspDTI ATGAA 8 cut(s) 42, 96, 177, 251, 1360, 1476, 1557, 2673
TspGWI ACGGA 3 cut(s) 390, 1645, 2581
VneI GTGCAC 1 cut(s) 1889
VpaK11BI GGWCC 3 cut(s) 1040, 1184, 1760
XapI RAATTY 3 cut(s) 928, 1702, 2323
XceI RCATGY 1 cut(s) 2837
XmiI GTMKAC 1 cut(s) 2748
XmnI GAANNNNTTC 3 cut(s) 527, 599, 2763
XspI CTAG 3 cut(s) 1754, 2243, 3040
ZraI GACGTC 2 cut(s) 1090, 2752
ZrmI AGTACT 1 cut(s) 2086
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.