MD05G1194300.v1.1

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0016262
Physical Location & Seq
Reverse (-)
32192340 .. 32195288
2949 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1194300.v1.1.491

Sequence Viewer

Length: 1707 bp
ATGTTAGCTTTTTCTGTATGTCTTGGATATGCCGGCCATCCCTTCCATACCAAACAACTGCCAAAATTCTGCTGTCATGCGGCCACCCCCTTTAAAAACAAACTCCATCTCTTCATTACCACTCCCCTTCATCGTTTTCTTTCCCTACCAATCTTACTGAAACCAGAATTTCAAATGGCTGATGGGACTGTGCATATAATCCAATCCCACCAACCTTGGATTAATTACCCCAACCCTAATGTCAATGGCTTCCAAAATAATCCCAACTCCGATCCCAAGCCCGTCGAGAACCGGTTTATAAACTTGGAGAGGAGTGAACTATCTCTGTGGGTGGAGCACATATCAAAACAGCTCATCGACGACTTGCCAGAACCCGCCACAGCCGCCAACACCGCCGCCAATGACACGTTACAGACGTCTGAGGACTATTTTGTCCCGTCATCACTTTCCACAGACTTAAATAGTCCGAGGAAAGTCCCACGGAGAAGCTATGGTGAGCATGAGCTTCATGAGCTCCAGCGGGGCAATGAGCTTCAACTCCTTGCAGATAATAATAACGTAAGAGCAAGAGGAGGGAATGATATGAGTAAGCTCGATGAGCATGGCTTAACCCTAATCACCCTTCTCTTCGAATGCGCAGTTGCCATCTCCGTTGACAACCTCCCCGAGGCCCATCGGATGCTCCTCGAGCTAACCCAAATGGCCTCTCCGTACGGGCCATCATGCGCTGAGCGCGTGGTGGCCTATTTCGCCAAGGCCATGGCTAGTAGGGTTATCAACTCATGGTTAGGCATTTCCTCTCCATTGGTCAACTACAAAAGCGTTCATGGTGCTTTTCAAGTCTTCAACACCATCTCCCCTTTCATCAAATTTGCCCACTTCACTTCCAACCAAGCCATCCTCGAGGCCTTTCACCACCACGATATGGTTCACATTCTTGACCTCGACATCATGCAGGGTTTGCAATGGCCAGCCTTGTTTCACATTCTCGCCACACGCATGGAGGGTCCTCCACATGTGAGAATGACCGGCATGGGCACATCATCGGAAATCCTGCTGGAGACCGGAAAGCAACTCTCAAGTTTTGCAAGGAGACTCGGACTGTCATTCGAATTTCATCCAATTGTCAGGAAAGTTGGTGAGGTAGATGCTTCCATGGTCCAAGTACGGCGAGGGGAGACCCTCGCCGTGCACTGGCTGCAACACTCGCTGTACGATGCAACTGGCCCCGATTGGAAAGCGATGAGATTCATTGAAGAATTAGCACCAAGGATTATCACGCTAGTAGAGCAAGACATGTCACATAGTGGCTCATTTTTGGACCGTTTTGTTGGGTCGTTACATTACTACTCGACGATGTTCGATTCACTAGAATCATACTTGCCTAGTGACAACCCTAACCGGCACAAGGTGGAGCACTGCCTGTTTTATAGAGAAATCAACAACATATTGGCGATTGGAGGGCCGGCGAGAAGCGGTGAAGACAAGTTCCGGCAGTGGAGAAGTGAGCTTGGGGCCAGAAGTGAGTTCATGCAAGTAGGGATGAGTGGGAATTCAATGGCACAAGCACAGTTGATATTAAATATGTTCCCTCCTACACATGGGTATAGCCTTGTGCAAGGAGATGGCACTATTAGGCTTGGGTGGAAGGATACTAGTTTGTATGTTGCTTCTGCATGGACATGCTCTCATGCTTCTAAGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

569

Amino Acids

63.97

Weight (kDa)

6.42

Isoelectric Point (pI)

46.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 206 - 561 3.4e-108 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016262)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g21170
malus_domestica MD05G1194300.v1.1 MD10G1181000.v1.1
prunus_persica Prupe.8G220900_v2.0.a1
pyrus_communis pycom10g15680
rosa_chinensis RchiOBHm_Chr6g0287671
rosa_laevigata RLG00000012475
rosa_multiflora Rmu_sc0001813.1_g000002
rosa_roxburghii Rroxscaffold_7G00181300
rosa_rugosa Rorug06G0190500
rosa_samantha Rh6AG301000 Rh6CG302800 Rh6CG314900 Rh6DG298400
rosa_wichuraiana Rw6G025970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 299
AasI GACNNNNNNGTC 1 cut(s) 431
AatII GACGTC 1 cut(s) 419
AbsI CCTCGAGG 1 cut(s) 902
Acc16I TGCGCA 1 cut(s) 637
AccB7I CCANNNNNTGG 1 cut(s) 925
AccII CGCG 1 cut(s) 735
AciI CCGC 7 cut(s) 80, 375, 384, 393, 396, 520, 1476
AclWI GGATC 1 cut(s) 266
AcoI YGGCCR 3 cut(s) 34, 81, 968
AcsI RAATTY 5 cut(s) 65, 167, 869, 1112, 1552
AcyI GRCGYC 1 cut(s) 416
AdeI CACNNNGTG 2 cut(s) 1307, 1411
AfaI GTAC 3 cut(s) 713, 1167, 1214
AfiI CCNNNNNNNGG 5 cut(s) 667, 925, 1194, 1408, 1601
AflIII ACRYGT 3 cut(s) 405, 1015, 1296
AgeI ACCGGT 1 cut(s) 291
AgsI TTSAA 6 cut(s) 173, 536, 839, 847, 1256, 1557
AhlI ACTAGT 1 cut(s) 1655
AluBI AGCT 9 cut(s) 8, 352, 489, 505, 514, 532, 592, 691, 1510
AluI AGCT 9 cut(s) 8, 352, 489, 505, 514, 532, 592, 691, 1510
Alw21I GWGCWC 4 cut(s) 339, 516, 1194, 1419
Alw26I GTCTC 3 cut(s) 1055, 1087, 1172
Alw44I GTGCAC 1 cut(s) 1190
AlwI GGATC 1 cut(s) 266
Ama87I CYCGRG 3 cut(s) 665, 686, 902
ApaLI GTGCAC 1 cut(s) 1190
ApeKI GCWGC 1 cut(s) 1198
ApoI RAATTY 5 cut(s) 65, 167, 869, 1112, 1552
AseI ATTAAT 1 cut(s) 222
AsiGI ACCGGT 1 cut(s) 291
AspLEI GCGC 3 cut(s) 638, 728, 735
AspS9I GGNCC 8 cut(s) 670, 716, 1007, 1159, 1226, 1321, 1463, 1515
AsuHPI GGTGA 5 cut(s) 506, 610, 905, 1151, 1490
AsuII TTCGAA 2 cut(s) 630, 1110
AvaI CYCGRG 3 cut(s) 665, 686, 902
AvaII GGWCC 3 cut(s) 1007, 1159, 1321
BaeGI GKGCMC 2 cut(s) 1040, 1194
BalI TGGCCA 1 cut(s) 970
BanII GRGCYC 1 cut(s) 516
BbsI GAAGAC 2 cut(s) 835, 1488
Bbv12I GWGCWC 4 cut(s) 339, 516, 1194, 1419
BbvI GCAGC 1 cut(s) 1185
BccI CCATC 9 cut(s) 45, 114, 176, 653, 681, 727, 860, 905, 1619
BceAI ACGGC 2 cut(s) 1172, 1184
BciVI GTATCC 1 cut(s) 1645
BcoDI GTCTC 3 cut(s) 1055, 1087, 1172
BcuI ACTAGT 1 cut(s) 1655
BfaI CTAG 5 cut(s) 765, 1283, 1370, 1386, 1656
BfuI GTATCC 1 cut(s) 1645
BisI GCNGC 4 cut(s) 81, 384, 396, 1199
BlpI GCTNAGC 1 cut(s) 729
BlsI GCNGC 4 cut(s) 82, 385, 397, 1200
Bme18I GGWCC 3 cut(s) 1007, 1159, 1321
BmeT110I CYCGRG 3 cut(s) 665, 686, 902
BmgT120I GGNCC 8 cut(s) 670, 716, 1007, 1159, 1226, 1321, 1463, 1515
BmiI GGNNCC 3 cut(s) 1008, 1228, 1516
BmsI GCATC 3 cut(s) 669, 1138, 1207
BpiI GAAGAC 2 cut(s) 835, 1488
BpmI CTGGAG 2 cut(s) 500, 1079
Bpu1102I GCTNAGC 1 cut(s) 729
Bpu14I TTCGAA 2 cut(s) 630, 1110
BpuEI CTTGAG 1 cut(s) 1063
BsaHI GRCGYC 1 cut(s) 416
BsaI GGTCTC 2 cut(s) 1055, 1172
BsaJI CCNNGG 8 cut(s) 215, 467, 479, 666, 753, 759, 1155, 1268
BsaWI WCCGGW 2 cut(s) 291, 1064
BsaXI ACNNNNNCTCC 2 cut(s) 839, 869
Bsc4I CCNNNNNNNGG 5 cut(s) 667, 925, 1194, 1408, 1601
Bse118I RCCGGY 5 cut(s) 32, 291, 1028, 1401, 1465
Bse1I ACTGG 2 cut(s) 1199, 1228
Bse3DI GCAATG 2 cut(s) 532, 971
BseDI CCNNGG 8 cut(s) 215, 467, 479, 666, 753, 759, 1155, 1268
BseGI GGATG 5 cut(s) 37, 684, 897, 1117, 1548
BseLI CCNNNNNNNGG 5 cut(s) 667, 925, 1194, 1408, 1601
BseMI GCAATG 2 cut(s) 532, 971
BseMII CTCAG 2 cut(s) 411, 720
BseNI ACTGG 2 cut(s) 1199, 1228
BseRI GAGGAG 3 cut(s) 325, 585, 674
BseSI GKGCMC 2 cut(s) 1040, 1194
BseXI GCAGC 1 cut(s) 1185
Bsh1236I CGCG 1 cut(s) 735
BshTI ACCGGT 1 cut(s) 291
BsiHKAI GWGCWC 4 cut(s) 339, 516, 1194, 1419
BsiHKCI CYCGRG 3 cut(s) 665, 686, 902
BsiSI CCGG 7 cut(s) 33, 292, 1029, 1065, 1402, 1466, 1492
BsiWI CGTACG 1 cut(s) 711
BslFI GGGAC 3 cut(s) 199, 419, 461
BslI CCNNNNNNNGG 5 cut(s) 667, 925, 1194, 1408, 1601
BsmAI GTCTC 3 cut(s) 1055, 1087, 1172
BsmFI GGGAC 3 cut(s) 199, 419, 461
BsmI GAATGC 1 cut(s) 638
Bso31I GGTCTC 2 cut(s) 1055, 1172
BsoBI CYCGRG 3 cut(s) 665, 686, 902
Bsp119I TTCGAA 2 cut(s) 630, 1110
Bsp1286I GDGCHC 5 cut(s) 339, 516, 1040, 1194, 1419
Bsp143I GATC 1 cut(s) 271
Bsp1720I GCTNAGC 1 cut(s) 729
Bsp19I CCATGG 2 cut(s) 759, 1155
BspACI CCGC 7 cut(s) 80, 375, 384, 393, 396, 520, 1476
BspCNI CTCAG 2 cut(s) 412, 721
BspFNI CGCG 1 cut(s) 735
BspHI TCATGA 1 cut(s) 508
BspLI GGNNCC 3 cut(s) 1008, 1228, 1516
BspPI GGATC 1 cut(s) 266
BspT104I TTCGAA 2 cut(s) 630, 1110
BspTNI GGTCTC 2 cut(s) 1055, 1172
BsrDI GCAATG 2 cut(s) 532, 971
BsrFI RCCGGY 5 cut(s) 32, 291, 1028, 1401, 1465
BsrI ACTGG 2 cut(s) 1199, 1228
BssAI RCCGGY 5 cut(s) 32, 291, 1028, 1401, 1465
BssECI CCNNGG 8 cut(s) 215, 467, 479, 666, 753, 759, 1155, 1268
BssMI GATC 1 cut(s) 271
BssNI GRCGYC 1 cut(s) 416
BssT1I CCWWGG 5 cut(s) 215, 753, 759, 1155, 1268
Bst4CI ACNGT 4 cut(s) 190, 1104, 1325, 1572
Bst6I CTCTTC 2 cut(s) 116, 632
BstACI GRCGYC 1 cut(s) 416
BstAPI GCANNNNNTGC 2 cut(s) 961, 1198
BstBI TTCGAA 2 cut(s) 630, 1110
BstC8I GCNNGC 3 cut(s) 34, 972, 1467
BstDEI CTNAG 3 cut(s) 420, 729, 1698
BstDSI CCRYGG 3 cut(s) 479, 759, 1155
BstENI CCTNNNNNAGG 1 cut(s) 665
BstF5I GGATG 5 cut(s) 37, 684, 897, 1117, 1548
BstFNI CGCG 1 cut(s) 735
BstHHI GCGC 3 cut(s) 638, 728, 735
BstKTI GATC 1 cut(s) 274
BstMAI GTCTC 3 cut(s) 1055, 1087, 1172
BstMBI GATC 1 cut(s) 271
BstNSI RCATGY 3 cut(s) 1019, 1300, 1686
BstSLI GKGCMC 2 cut(s) 1040, 1194
BstUI CGCG 1 cut(s) 735
BstV1I GCAGC 1 cut(s) 1185
BstV2I GAAGAC 2 cut(s) 835, 1488
BstXI CCANNNNNNTGG 2 cut(s) 760, 1000
BsuI GTATCC 1 cut(s) 1645
BtgI CCRYGG 3 cut(s) 479, 759, 1155
BtgZI GCGATG 1 cut(s) 1256
BtsCI GGATG 5 cut(s) 37, 684, 897, 1117, 1548
BtsI GCAGTG 2 cut(s) 1417, 1502
BtsIMutI CAGTG 3 cut(s) 1192, 1417, 1502
Cac8I GCNNGC 3 cut(s) 34, 972, 1467
CciI TCATGA 1 cut(s) 508
CfoI GCGC 3 cut(s) 638, 728, 735
Cfr10I RCCGGY 5 cut(s) 32, 291, 1028, 1401, 1465
Cfr13I GGNCC 8 cut(s) 670, 716, 1007, 1159, 1226, 1321, 1463, 1515
Csp6I GTAC 3 cut(s) 712, 1166, 1213
CspAI ACCGGT 1 cut(s) 291
CspCI CAANNNNNGTGG 2 cut(s) 197, 232
CviQI GTAC 3 cut(s) 712, 1166, 1213
DdeI CTNAG 3 cut(s) 420, 729, 1698
DpnI GATC 1 cut(s) 273
DpnII GATC 1 cut(s) 271
DraI TTTAAA 1 cut(s) 94
DraIII CACNNNGTG 2 cut(s) 1307, 1411
DrdI GACNNNNNNGTC 1 cut(s) 431
DseDI GACNNNNNNGTC 1 cut(s) 431
EaeI YGGCCR 3 cut(s) 34, 81, 968
Eam1104I CTCTTC 2 cut(s) 116, 632
EarI CTCTTC 2 cut(s) 116, 632
Ecl136II GAGCTC 1 cut(s) 514
Eco130I CCWWGG 5 cut(s) 215, 753, 759, 1155, 1268
Eco147I AGGCCT 1 cut(s) 908
Eco24I GRGCYC 1 cut(s) 516
Eco31I GGTCTC 2 cut(s) 1055, 1172
Eco47I GGWCC 3 cut(s) 1007, 1159, 1321
Eco53kI GAGCTC 1 cut(s) 514
Eco88I CYCGRG 3 cut(s) 665, 686, 902
EcoICRI GAGCTC 1 cut(s) 514
EcoNI CCTNNNNNAGG 1 cut(s) 665
EcoO109I RGGNCCY 1 cut(s) 1007
EcoRI GAATTC 1 cut(s) 1552
EcoT14I CCWWGG 5 cut(s) 215, 753, 759, 1155, 1268
EcoT38I GRGCYC 1 cut(s) 516
ErhI CCWWGG 5 cut(s) 215, 753, 759, 1155, 1268
FaqI GGGAC 3 cut(s) 199, 419, 461
FauI CCCGC 2 cut(s) 382, 513
Fnu4HI GCNGC 4 cut(s) 81, 384, 396, 1199
FokI GGATG 5 cut(s) 24, 691, 884, 1104, 1555
FriOI GRGCYC 1 cut(s) 516
Fsp4HI GCNGC 4 cut(s) 81, 384, 396, 1199
FspBI CTAG 5 cut(s) 765, 1283, 1370, 1386, 1656
FspI TGCGCA 1 cut(s) 637
GlaI GCGC 3 cut(s) 637, 727, 734
GluI GCNGC 4 cut(s) 81, 384, 396, 1199
GsuI CTGGAG 2 cut(s) 500, 1079
HapII CCGG 7 cut(s) 33, 292, 1029, 1065, 1402, 1466, 1492
HhaI GCGC 3 cut(s) 638, 728, 735
Hin1I GRCGYC 1 cut(s) 416
Hin6I GCGC 3 cut(s) 636, 726, 733
HinP1I GCGC 3 cut(s) 636, 726, 733
HincII GTYRAC 2 cut(s) 655, 811
HindII GTYRAC 2 cut(s) 655, 811
HinfI GANTC 4 cut(s) 1095, 1248, 1364, 1373
HpaII CCGG 7 cut(s) 33, 292, 1029, 1065, 1402, 1466, 1492
HphI GGTGA 5 cut(s) 506, 610, 905, 1151, 1490
Hpy166II GTNNAC 5 cut(s) 317, 655, 811, 931, 1192
Hpy188I TCNGA 6 cut(s) 271, 421, 468, 678, 1048, 1100
Hpy188III TCNNGA 4 cut(s) 286, 509, 938, 1129
Hpy8I GTNNAC 5 cut(s) 317, 655, 811, 931, 1192
Hpy99I CGWCG 3 cut(s) 287, 362, 1357
HpyAV CCTTC 4 cut(s) 52, 137, 632, 1642
HpyCH4III ACNGT 4 cut(s) 190, 1104, 1325, 1572
HpyCH4IV ACGT 3 cut(s) 407, 416, 558
HpyF3I CTNAG 3 cut(s) 420, 729, 1698
HpySE526I ACGT 3 cut(s) 407, 416, 558
Hsp92I GRCGYC 1 cut(s) 416
HspAI GCGC 3 cut(s) 636, 726, 733
KroI GCCGGC 2 cut(s) 32, 1465
KroNI GCCGGC 2 cut(s) 34, 1467
Kzo9I GATC 1 cut(s) 271
LmnI GCTCC 4 cut(s) 334, 519, 687, 1414
Lsp1109I GCAGC 1 cut(s) 1185
LweI GCATC 3 cut(s) 669, 1138, 1207
MaeI CTAG 5 cut(s) 765, 1283, 1370, 1386, 1656
MaeII ACGT 3 cut(s) 407, 416, 558
MaeIII GTNAC 4 cut(s) 408, 1299, 1338, 1388
MalI GATC 1 cut(s) 273
MboI GATC 1 cut(s) 271
MboII GAAGA 5 cut(s) 103, 619, 835, 1268, 1493
MfeI CAATTG 1 cut(s) 1122
MhlI GDGCHC 5 cut(s) 339, 516, 1040, 1194, 1419
MlsI TGGCCA 1 cut(s) 970
MluCI AATT 8 cut(s) 65, 167, 223, 869, 1112, 1122, 1259, 1552
MluNI TGGCCA 1 cut(s) 970
MlyI GAGTC 1 cut(s) 1089
MmeI TCCRAC 1 cut(s) 912
Mox20I TGGCCA 1 cut(s) 970
MroNI GCCGGC 2 cut(s) 32, 1465
MscI TGGCCA 1 cut(s) 970
MseI TTAA 5 cut(s) 93, 222, 458, 608, 1580
MslI CAYNNNNRTG 2 cut(s) 998, 1681
Msp20I TGGCCA 1 cut(s) 970
MspA1I CMGCKG 1 cut(s) 520
MspI CCGG 7 cut(s) 33, 292, 1029, 1065, 1402, 1466, 1492
MunI CAATTG 1 cut(s) 1122
Mva1269I GAATGC 1 cut(s) 638
MvnI CGCG 1 cut(s) 735
NaeI GCCGGC 2 cut(s) 34, 1467
NcoI CCATGG 2 cut(s) 759, 1155
NdeII GATC 1 cut(s) 271
NgoMIV GCCGGC 2 cut(s) 32, 1465
NlaIV GGNNCC 3 cut(s) 1008, 1228, 1516
NmuCI GTSAC 2 cut(s) 1299, 1388
NsbI TGCGCA 1 cut(s) 637
NspI RCATGY 3 cut(s) 1019, 1300, 1686
NspV TTCGAA 2 cut(s) 630, 1110
PaeR7I CTCGAG 2 cut(s) 686, 902
PagI TCATGA 1 cut(s) 508
PceI AGGCCT 1 cut(s) 908
PciI ACATGT 2 cut(s) 1015, 1296
PcsI WCGNNNNNNNCGW 1 cut(s) 413
PctI GAATGC 1 cut(s) 638
PdiI GCCGGC 2 cut(s) 34, 1467
PfeI GAWTC 3 cut(s) 1248, 1364, 1373
Pfl23II CGTACG 1 cut(s) 711
PflMI CCANNNNNTGG 1 cut(s) 925
PinAI ACCGGT 1 cut(s) 291
PkrI GCNGC 4 cut(s) 82, 385, 397, 1200
PleI GAGTC 1 cut(s) 1089
PpsI GAGTC 1 cut(s) 1089
PpuMI RGGWCCY 1 cut(s) 1007
PscI ACATGT 2 cut(s) 1015, 1296
PshBI ATTAAT 1 cut(s) 222
PsiI TTATAA 1 cut(s) 299
Psp124BI GAGCTC 1 cut(s) 516
Psp5II RGGWCCY 1 cut(s) 1007
PspLI CGTACG 1 cut(s) 711
PspN4I GGNNCC 3 cut(s) 1008, 1228, 1516
PspPI GGNCC 8 cut(s) 670, 716, 1007, 1159, 1226, 1321, 1463, 1515
PspPPI RGGWCCY 1 cut(s) 1007
PspXI VCTCGAGB 2 cut(s) 686, 902
RsaI GTAC 3 cut(s) 713, 1167, 1214
RsaNI GTAC 3 cut(s) 712, 1166, 1213
RseI CAYNNNNRTG 2 cut(s) 998, 1681
SacI GAGCTC 1 cut(s) 516
SaqAI TTAA 5 cut(s) 93, 222, 458, 608, 1580
SatI GCNGC 4 cut(s) 81, 384, 396, 1199
Sau3AI GATC 1 cut(s) 271
Sau96I GGNCC 8 cut(s) 670, 716, 1007, 1159, 1226, 1321, 1463, 1515
SchI GAGTC 1 cut(s) 1089
SduI GDGCHC 5 cut(s) 339, 516, 1040, 1194, 1419
SfaNI GCATC 3 cut(s) 669, 1138, 1207
Sfr274I CTCGAG 2 cut(s) 686, 902
SfuI TTCGAA 2 cut(s) 630, 1110
SinI GGWCC 3 cut(s) 1007, 1159, 1321
SlaI CTCGAG 2 cut(s) 686, 902
SmiMI CAYNNNNRTG 2 cut(s) 998, 1681
SmlI CTYRAG 3 cut(s) 686, 902, 1078
SmoI CTYRAG 3 cut(s) 686, 902, 1078
SpeI ACTAGT 1 cut(s) 1655
Sse9I AATT 8 cut(s) 65, 167, 223, 869, 1112, 1122, 1259, 1552
SseBI AGGCCT 1 cut(s) 908
SsiI CCGC 7 cut(s) 80, 375, 384, 393, 396, 520, 1476
SspMI CTAG 5 cut(s) 765, 1283, 1370, 1386, 1656
SstI GAGCTC 1 cut(s) 516
StuI AGGCCT 1 cut(s) 908
StyI CCWWGG 5 cut(s) 215, 753, 759, 1155, 1268
TaaI ACNGT 4 cut(s) 190, 1104, 1325, 1572
TaiI ACGT 3 cut(s) 410, 419, 561
TasI AATT 8 cut(s) 65, 167, 223, 869, 1112, 1122, 1259, 1552
TauI GCSGC 3 cut(s) 83, 386, 398
TfiI GAWTC 3 cut(s) 1248, 1364, 1373
Tru1I TTAA 5 cut(s) 93, 222, 458, 608, 1580
Tru9I TTAA 5 cut(s) 93, 222, 458, 608, 1580
TscAI CASTG 3 cut(s) 1199, 1424, 1502
TseFI GTSAC 2 cut(s) 1299, 1388
TseI GCWGC 1 cut(s) 1198
Tsp45I GTSAC 2 cut(s) 1299, 1388
TspDTI ATGAA 8 cut(s) 103, 119, 497, 815, 853, 1106, 1240, 1519
TspGWI ACGGA 3 cut(s) 496, 640, 699
TspRI CASTG 3 cut(s) 1199, 1424, 1502
Van91I CCANNNNNTGG 1 cut(s) 925
VneI GTGCAC 1 cut(s) 1190
VpaK11BI GGWCC 3 cut(s) 1007, 1159, 1321
VspI ATTAAT 1 cut(s) 222
XagI CCTNNNNNAGG 1 cut(s) 665
XapI RAATTY 5 cut(s) 65, 167, 869, 1112, 1552
XceI RCATGY 3 cut(s) 1019, 1300, 1686
XhoI CTCGAG 2 cut(s) 686, 902
XspI CTAG 5 cut(s) 765, 1283, 1370, 1386, 1656
ZraI GACGTC 1 cut(s) 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.