RLG00000012475

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0016262
Physical Location & Seq
Forward (+)
19138026 .. 19139861
1836 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012475

Sequence Viewer

Length: 1491 bp
ATGGTTTATGGGACTGTGAATATGATCCAACCCCATGAAACTAATTGGCTTAACAATTCTACCTCCGTTAATCCATTCCCAAAGCCTGTAAGCGATCTGTCTCAGTGGGTAGAGCACCTAACGAAGCAACTCATCGATGACTTGCCGGAAACAGCCACCACCACCGCAGACACTTTGCACACTGCAGCAACACCCGGGGATCAGTACTTCATCCCCTCGTCACATTCAGGAGAGTTTAGTCCGAGAAAATCCCCCAGGAAAAACTACGGCGACGACACAAGTGCTGATCGTGCAGATTACGACTTGCAGTGGGGAAATGAGCTGCAACTCCATGCAGAAAACAACAGGAGAGCTAGAGGGTTGAGTAAGCTTGACCAGCATGGCTTAACACTAATTACTCTTCTGTTCGAATGTGCGGTTGCAATCTCCGTGGAGAACCCCGGCGAGGCACATCGGATGCTGCTCGAGCTCACCCAGATGGCCTCGCCGTATGGGCCATCCTGCGCGGAGCGCGTGGTGGCATATTTTGCCAAGGCCATGTCGAGTAGGGTTTTGAACTCGTGGCTAGGCATTTGCTCTCCTTTGGTCAACTACAAAAGCATTCATGGTGCTTTTCAAGTCTTAAACACTATCTCGCCTTTCATCAAGTTCGCTCACTTCACTTCCAACCAAGCCATCCTCGAAGCCTTTCACCGCCGTGACAGGGTGCACATTATCGACCTCGATATCATGCAGGGTTTGCAATGGCCAGCCCTTTTTCACATCCTCGCCACACGCATGGAAGGGCCTCCACACGTACGAATGACCGGCTTCGGCGCCTCATCTGAGGAATTGCTTGAGACCGGAAAGCAACTTTCAAATTTTGCCAGGCGACTGGGCTTGTCATTTGAGTTTCATCCAATCACGAGGAAATTCGGTGACCACCTAGACACTTCAATGGTGCAAGTGCGGAGGGGAGAGACTGTAGCAGTGCATTGGCTACAGCACTCGCTCTACGATGCAACAGGACCCGACTGGAGAACGATGAAACTCATCGAAGAACTATCCCCGAGAATCGTCACATTGGTAGAGCAAGACATGTCGCACAATGGGGGCTCGTTTTTGGACCGTTTTGTCGGGTCTTTGCACTACTATTCAACTATGTTTGATTCTCTTGAAGCCTACTTGCCCAGTGATAACCCTAACAGGCATTTAGTTGAACACAGCTTGATGTATAGGGAAATCAACAACATATTGGCAGTAGGCGGGCCAGCAAGGAACGGAGAAGACAAGTTTAGGCATTGGAGGAGTGAGCTCGGGGCTAGCAATAGCTTCATGCAAGTAGGTATGAGTGGAAATTCGATGGCTCAAGCGCAGTTGATATTGAACATGTTTCCTCCTAGTAATGGCTATAACCTTGTGCATGGAGATGGGACGATTAGGCTTGGGTGGAAGAATACTAGTTTATATGTGGCCTCAGCATGGACTTGTTCTCATGCTTCATCAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

497

Amino Acids

55.73

Weight (kDa)

6.31

Isoelectric Point (pI)

39.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 132 - 489 3.8e-111 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016262)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g21170
malus_domestica MD05G1194300.v1.1 MD10G1181000.v1.1
prunus_persica Prupe.8G220900_v2.0.a1
pyrus_communis pycom10g15680
rosa_chinensis RchiOBHm_Chr6g0287671
rosa_laevigata RLG00000012475
rosa_multiflora Rmu_sc0001813.1_g000002
rosa_roxburghii Rroxscaffold_7G00181300
rosa_rugosa Rorug06G0190500
rosa_samantha Rh6AG301000 Rh6CG302800 Rh6CG314900 Rh6DG298400
rosa_wichuraiana Rw6G025970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1112
AccB1I GGYRCC 1 cut(s) 815
AccII CGCG 2 cut(s) 506, 513
AciI CCGC 6 cut(s) 165, 416, 506, 694, 949, 1245
AclWI GGATC 2 cut(s) 19, 207
AcoI YGGCCR 1 cut(s) 746
AcsI RAATTY 3 cut(s) 859, 911, 1336
AcyI GRCGYC 1 cut(s) 816
AfaI GTAC 2 cut(s) 206, 798
AfiI CCNNNNNNNGG 4 cut(s) 445, 703, 1385, 1461
AflIII ACRYGT 3 cut(s) 793, 1077, 1368
AgsI TTSAA 8 cut(s) 556, 617, 858, 936, 1137, 1157, 1199, 1366
AhlI ACTAGT 1 cut(s) 1439
AjnI CCWGG 2 cut(s) 254, 866
AleI CACNNNNGTG 1 cut(s) 696
AluBI AGCT 7 cut(s) 322, 353, 370, 469, 1206, 1294, 1311
AluI AGCT 7 cut(s) 322, 353, 370, 469, 1206, 1294, 1311
Alw21I GWGCWC 4 cut(s) 117, 471, 711, 1296
Alw26I GTCTC 3 cut(s) 105, 833, 953
Alw44I GTGCAC 1 cut(s) 707
AlwI GGATC 2 cut(s) 19, 207
Ama87I CYCGRG 4 cut(s) 194, 464, 1048, 1295
AoxI GGCC 7 cut(s) 480, 494, 534, 746, 785, 1247, 1452
ApaLI GTGCAC 1 cut(s) 707
ApeKI GCWGC 3 cut(s) 185, 322, 460
ApoI RAATTY 3 cut(s) 859, 911, 1336
Asp700I GAANNNNTTC 1 cut(s) 687
AspLEI GCGC 4 cut(s) 506, 513, 818, 1354
AspS9I GGNCC 5 cut(s) 494, 785, 1007, 1105, 1247
AsuC2I CCSGG 3 cut(s) 195, 196, 441
AsuHPI GGTGA 3 cut(s) 463, 683, 929
AsuII TTCGAA 1 cut(s) 408
AsuNHI GCTAGC 1 cut(s) 1301
AvaI CYCGRG 4 cut(s) 194, 464, 1048, 1295
AvaII GGWCC 2 cut(s) 1007, 1105
BaeGI GKGCMC 1 cut(s) 711
BalI TGGCCA 1 cut(s) 748
BanI GGYRCC 1 cut(s) 815
BanII GRGCYC 3 cut(s) 471, 1097, 1296
BauI CACGAG 2 cut(s) 559, 904
BbsI GAAGAC 1 cut(s) 1272
Bbv12I GWGCWC 4 cut(s) 117, 471, 711, 1296
BbvCI CCTCAGC 1 cut(s) 1456
BbvI GCAGC 3 cut(s) 197, 309, 447
BccI CCATC 5 cut(s) 472, 505, 683, 1336, 1403
BceAI ACGGC 3 cut(s) 283, 472, 681
BcgI CGANNNNNNTGC 2 cut(s) 263, 297
BciT130I CCWGG 2 cut(s) 256, 868
BcnI CCSGG 3 cut(s) 195, 196, 441
BcoDI GTCTC 3 cut(s) 105, 833, 953
BcuI ACTAGT 1 cut(s) 1439
BfaI CTAG 6 cut(s) 354, 566, 926, 1302, 1380, 1440
BfmI CTRYAG 3 cut(s) 183, 963, 980
BfoI RGCGCY 1 cut(s) 819
BglI GCCNNNNNGGC 1 cut(s) 493
BisI GCNGC 3 cut(s) 186, 323, 461
BlsI GCNGC 3 cut(s) 187, 324, 462
BmcAI AGTACT 1 cut(s) 206
Bme1390I CCNGG 5 cut(s) 195, 196, 256, 441, 868
Bme18I GGWCC 2 cut(s) 1007, 1105
BmeT110I CYCGRG 4 cut(s) 194, 464, 1048, 1295
BmgT120I GGNCC 5 cut(s) 494, 785, 1007, 1105, 1247
BmiI GGNNCC 2 cut(s) 817, 1009
BmrFI CCNGG 5 cut(s) 195, 196, 256, 441, 868
BmrI ACTGGG 2 cut(s) 884, 1164
BmsI GCATC 2 cut(s) 447, 988
BmtI GCTAGC 1 cut(s) 1305
BmuI ACTGGG 2 cut(s) 884, 1164
BpiI GAAGAC 1 cut(s) 1272
BpmI CTGGAG 1 cut(s) 1036
Bpu10I CCTNAGC 1 cut(s) 1456
Bpu14I TTCGAA 1 cut(s) 408
BpuEI CTTGAG 2 cut(s) 857, 1332
BpuMI CCSGG 3 cut(s) 195, 196, 441
Bsa29I ATCGAT 1 cut(s) 135
BsaAI YACGTR 1 cut(s) 796
BsaHI GRCGYC 1 cut(s) 816
BsaI GGTCTC 1 cut(s) 833
BsaJI CCNNGG 6 cut(s) 194, 195, 254, 429, 439, 531
BsaWI WCCGGW 1 cut(s) 842
BsaXI ACNNNNNCTCC 2 cut(s) 948, 978
Bsc4I CCNNNNNNNGG 4 cut(s) 445, 703, 1385, 1461
Bse118I RCCGGY 1 cut(s) 806
Bse1I ACTGG 3 cut(s) 879, 1019, 1170
Bse3DI GCAATG 1 cut(s) 749
BseBI CCWGG 2 cut(s) 256, 868
BseCI ATCGAT 1 cut(s) 135
BseDI CCNNGG 6 cut(s) 194, 195, 254, 429, 439, 531
BseGI GGATG 6 cut(s) 210, 462, 497, 675, 762, 895
BseLI CCNNNNNNNGG 4 cut(s) 445, 703, 1385, 1461
BseMI GCAATG 1 cut(s) 749
BseMII CTCAG 3 cut(s) 116, 816, 1470
BseNI ACTGG 3 cut(s) 879, 1019, 1170
BseRI GAGGAG 1 cut(s) 1300
BseSI GKGCMC 1 cut(s) 711
BseXI GCAGC 3 cut(s) 197, 309, 447
BsgI GTGCAG 1 cut(s) 312
Bsh1236I CGCG 2 cut(s) 506, 513
BshFI GGCC 7 cut(s) 482, 496, 536, 748, 787, 1249, 1454
BshNI GGYRCC 1 cut(s) 815
BshVI ATCGAT 1 cut(s) 135
BsiHKAI GWGCWC 4 cut(s) 117, 471, 711, 1296
BsiHKCI CYCGRG 4 cut(s) 194, 464, 1048, 1295
BsiSI CCGG 5 cut(s) 146, 195, 441, 807, 843
BsiWI CGTACG 1 cut(s) 796
BslFI GGGAC 2 cut(s) 25, 1426
BslI CCNNNNNNNGG 4 cut(s) 445, 703, 1385, 1461
BsmAI GTCTC 3 cut(s) 105, 833, 953
BsmFI GGGAC 2 cut(s) 25, 1426
BsmI GAATGC 1 cut(s) 600
BsnI GGCC 7 cut(s) 482, 496, 536, 748, 787, 1249, 1454
Bso31I GGTCTC 1 cut(s) 833
BsoBI CYCGRG 4 cut(s) 194, 464, 1048, 1295
Bsp119I TTCGAA 1 cut(s) 408
Bsp1286I GDGCHC 5 cut(s) 117, 471, 711, 1097, 1296
Bsp143I GATC 4 cut(s) 24, 94, 199, 286
BspACI CCGC 6 cut(s) 165, 416, 506, 694, 949, 1245
BspANI GGCC 7 cut(s) 482, 496, 536, 748, 787, 1249, 1454
BspCNI CTCAG 3 cut(s) 115, 817, 1469
BspDI ATCGAT 1 cut(s) 135
BspFNI CGCG 2 cut(s) 506, 513
BspLI GGNNCC 2 cut(s) 817, 1009
BspMAI CTGCAG 1 cut(s) 187
BspOI GCTAGC 1 cut(s) 1305
BspPI GGATC 2 cut(s) 19, 207
BspT104I TTCGAA 1 cut(s) 408
BspT107I GGYRCC 1 cut(s) 815
BspTNI GGTCTC 1 cut(s) 833
BsrDI GCAATG 1 cut(s) 749
BsrFI RCCGGY 1 cut(s) 806
BsrI ACTGG 3 cut(s) 879, 1019, 1170
BssAI RCCGGY 1 cut(s) 806
BssECI CCNNGG 6 cut(s) 194, 195, 254, 429, 439, 531
BssMI GATC 4 cut(s) 24, 94, 199, 286
BssNI GRCGYC 1 cut(s) 816
BssSI CACGAG 2 cut(s) 559, 904
BssT1I CCWWGG 1 cut(s) 531
Bst2BI CACGAG 2 cut(s) 559, 904
Bst2UI CCWGG 2 cut(s) 256, 868
Bst4CI ACNGT 3 cut(s) 16, 964, 1109
Bst6I CTCTTC 1 cut(s) 405
BstACI GRCGYC 1 cut(s) 816
BstAPI GCANNNNNTGC 2 cut(s) 527, 739
BstBAI YACGTR 1 cut(s) 796
BstBI TTCGAA 1 cut(s) 408
BstC8I GCNNGC 4 cut(s) 750, 1247, 1251, 1303
BstDEI CTNAG 3 cut(s) 102, 825, 1456
BstDSI CCRYGG 1 cut(s) 429
BstEII GGTNACC 1 cut(s) 917
BstF5I GGATG 6 cut(s) 210, 462, 497, 675, 762, 895
BstFNI CGCG 2 cut(s) 506, 513
BstH2I RGCGCY 1 cut(s) 819
BstHHI GCGC 4 cut(s) 506, 513, 818, 1354
BstKTI GATC 4 cut(s) 27, 97, 202, 289
BstMAI GTCTC 3 cut(s) 105, 833, 953
BstMBI GATC 4 cut(s) 24, 94, 199, 286
BstMWI GCNNNNNNNGC 7 cut(s) 290, 376, 466, 493, 510, 527, 739
BstNI CCWGG 2 cut(s) 256, 868
BstNSI RCATGY 2 cut(s) 1081, 1372
BstPI GGTNACC 1 cut(s) 917
BstSCI CCNGG 5 cut(s) 193, 194, 254, 439, 866
BstSFI CTRYAG 3 cut(s) 183, 963, 980
BstSLI GKGCMC 1 cut(s) 711
BstUI CGCG 2 cut(s) 506, 513
BstV1I GCAGC 3 cut(s) 197, 309, 447
BstV2I GAAGAC 1 cut(s) 1272
BstXI CCANNNNNNTGG 2 cut(s) 778, 874
Bsu15I ATCGAT 1 cut(s) 135
BsuRI GGCC 7 cut(s) 482, 496, 536, 748, 787, 1249, 1454
BsuTUI ATCGAT 1 cut(s) 135
BtgI CCRYGG 1 cut(s) 429
BtsCI GGATG 6 cut(s) 210, 462, 497, 675, 762, 895
BtsI GCAGTG 3 cut(s) 180, 314, 975
BtsIMutI CAGTG 5 cut(s) 110, 180, 314, 975, 1177
Cac8I GCNNGC 4 cut(s) 750, 1247, 1251, 1303
CfoI GCGC 4 cut(s) 506, 513, 818, 1354
Cfr10I RCCGGY 1 cut(s) 806
Cfr13I GGNCC 5 cut(s) 494, 785, 1007, 1105, 1247
Cfr9I CCCGGG 1 cut(s) 194
ClaI ATCGAT 1 cut(s) 135
Csp6I GTAC 2 cut(s) 205, 797
CspCI CAANNNNNGTGG 2 cut(s) 411, 446
CviQI GTAC 2 cut(s) 205, 797
DdeI CTNAG 3 cut(s) 102, 825, 1456
DinI GGCGCC 1 cut(s) 817
DpnI GATC 4 cut(s) 26, 96, 201, 288
DpnII GATC 4 cut(s) 24, 94, 199, 286
DrdI GACNNNNNNGTC 1 cut(s) 1112
DseDI GACNNNNNNGTC 1 cut(s) 1112
EaeI YGGCCR 1 cut(s) 746
Eam1104I CTCTTC 1 cut(s) 405
EarI CTCTTC 1 cut(s) 405
Ecl136II GAGCTC 2 cut(s) 469, 1294
Eco130I CCWWGG 1 cut(s) 531
Eco24I GRGCYC 3 cut(s) 471, 1097, 1296
Eco31I GGTCTC 1 cut(s) 833
Eco32I GATATC 1 cut(s) 727
Eco47I GGWCC 2 cut(s) 1007, 1105
Eco53kI GAGCTC 2 cut(s) 469, 1294
Eco88I CYCGRG 4 cut(s) 194, 464, 1048, 1295
Eco91I GGTNACC 1 cut(s) 917
EcoICRI GAGCTC 2 cut(s) 469, 1294
EcoO109I RGGNCCY 2 cut(s) 785, 1007
EcoO65I GGTNACC 1 cut(s) 917
EcoRII CCWGG 2 cut(s) 254, 866
EcoRV GATATC 1 cut(s) 727
EcoT14I CCWWGG 1 cut(s) 531
EcoT38I GRGCYC 3 cut(s) 471, 1097, 1296
EgeI GGCGCC 1 cut(s) 817
EheI GGCGCC 1 cut(s) 817
ErhI CCWWGG 1 cut(s) 531
FaqI GGGAC 2 cut(s) 25, 1426
FauI CCCGC 1 cut(s) 1238
Fnu4HI GCNGC 3 cut(s) 186, 323, 461
FokI GGATG 6 cut(s) 197, 469, 484, 662, 749, 882
FriOI GRGCYC 3 cut(s) 471, 1097, 1296
Fsp4HI GCNGC 3 cut(s) 186, 323, 461
FspBI CTAG 6 cut(s) 354, 566, 926, 1302, 1380, 1440
GlaI GCGC 4 cut(s) 505, 512, 817, 1353
GluI GCNGC 3 cut(s) 186, 323, 461
GsuI CTGGAG 1 cut(s) 1036
HaeII RGCGCY 1 cut(s) 819
HaeIII GGCC 7 cut(s) 482, 496, 536, 748, 787, 1249, 1454
HapII CCGG 5 cut(s) 146, 195, 441, 807, 843
HhaI GCGC 4 cut(s) 506, 513, 818, 1354
Hin1I GRCGYC 1 cut(s) 816
Hin6I GCGC 4 cut(s) 504, 511, 816, 1352
HinP1I GCGC 4 cut(s) 504, 511, 816, 1352
HincII GTYRAC 1 cut(s) 589
HindII GTYRAC 1 cut(s) 589
HindIII AAGCTT 1 cut(s) 368
HinfI GANTC 2 cut(s) 1053, 1148
HpaII CCGG 5 cut(s) 146, 195, 441, 807, 843
HphI GGTGA 3 cut(s) 463, 683, 929
Hpy166II GTNNAC 2 cut(s) 589, 709
Hpy188I TCNGA 3 cut(s) 243, 456, 826
Hpy188III TCNNGA 4 cut(s) 228, 904, 1154, 1485
Hpy8I GTNNAC 2 cut(s) 589, 709
Hpy99I CGWCG 1 cut(s) 275
HpyAV CCTTC 1 cut(s) 776
HpyCH4III ACNGT 3 cut(s) 16, 964, 1109
HpyCH4IV ACGT 1 cut(s) 795
HpyF10VI GCNNNNNNNGC 7 cut(s) 290, 376, 466, 493, 510, 527, 739
HpyF3I CTNAG 3 cut(s) 102, 825, 1456
HpySE526I ACGT 1 cut(s) 795
Hsp92I GRCGYC 1 cut(s) 816
HspAI GCGC 4 cut(s) 504, 511, 816, 1352
KasI GGCGCC 1 cut(s) 815
Kzo9I GATC 4 cut(s) 24, 94, 199, 286
LmnI GCTCC 1 cut(s) 508
Lsp1109I GCAGC 3 cut(s) 197, 309, 447
LweI GCATC 2 cut(s) 447, 988
MaeI CTAG 6 cut(s) 354, 566, 926, 1302, 1380, 1440
MaeII ACGT 1 cut(s) 795
MaeIII GTNAC 4 cut(s) 219, 698, 917, 1057
MalI GATC 4 cut(s) 26, 96, 201, 288
MboI GATC 4 cut(s) 24, 94, 199, 286
MboII GAAGA 4 cut(s) 392, 1049, 1277, 1444
MhlI GDGCHC 5 cut(s) 117, 471, 711, 1097, 1296
MlsI TGGCCA 1 cut(s) 748
MluCI AATT 7 cut(s) 43, 55, 393, 830, 859, 911, 1336
MluNI TGGCCA 1 cut(s) 748
Mly113I GGCGCC 1 cut(s) 816
MmeI TCCRAC 2 cut(s) 52, 690
Mox20I TGGCCA 1 cut(s) 748
MroXI GAANNNNTTC 1 cut(s) 687
MscI TGGCCA 1 cut(s) 748
MseI TTAA 4 cut(s) 51, 69, 386, 623
MslI CAYNNNNRTG 5 cut(s) 476, 696, 776, 935, 1407
Msp20I TGGCCA 1 cut(s) 748
MspI CCGG 5 cut(s) 146, 195, 441, 807, 843
MspR9I CCNGG 5 cut(s) 195, 196, 256, 441, 868
Mva1269I GAATGC 1 cut(s) 600
MvaI CCWGG 2 cut(s) 256, 868
MvnI CGCG 2 cut(s) 506, 513
MwoI GCNNNNNNNGC 7 cut(s) 290, 376, 466, 493, 510, 527, 739
NarI GGCGCC 1 cut(s) 816
NciI CCSGG 3 cut(s) 195, 196, 441
NdeII GATC 4 cut(s) 24, 94, 199, 286
NheI GCTAGC 1 cut(s) 1301
NlaIV GGNNCC 2 cut(s) 817, 1009
NmuCI GTSAC 4 cut(s) 219, 698, 917, 1057
NspI RCATGY 2 cut(s) 1081, 1372
NspV TTCGAA 1 cut(s) 408
OliI CACNNNNGTG 1 cut(s) 696
PaeR7I CTCGAG 1 cut(s) 464
PciI ACATGT 2 cut(s) 1077, 1368
PctI GAATGC 1 cut(s) 600
PdmI GAANNNNTTC 1 cut(s) 687
PfeI GAWTC 2 cut(s) 1053, 1148
Pfl23II CGTACG 1 cut(s) 796
PkrI GCNGC 3 cut(s) 187, 324, 462
PluTI GGCGCC 1 cut(s) 819
Ppu21I YACGTR 1 cut(s) 796
PpuMI RGGWCCY 1 cut(s) 1007
PscI ACATGT 2 cut(s) 1077, 1368
Psp124BI GAGCTC 2 cut(s) 471, 1296
Psp5II RGGWCCY 1 cut(s) 1007
Psp6I CCWGG 2 cut(s) 254, 866
PspEI GGTNACC 1 cut(s) 917
PspGI CCWGG 2 cut(s) 254, 866
PspLI CGTACG 1 cut(s) 796
PspN4I GGNNCC 2 cut(s) 817, 1009
PspPI GGNCC 5 cut(s) 494, 785, 1007, 1105, 1247
PspPPI RGGWCCY 1 cut(s) 1007
PspXI VCTCGAGB 1 cut(s) 464
PstI CTGCAG 1 cut(s) 187
RsaI GTAC 2 cut(s) 206, 798
RsaNI GTAC 2 cut(s) 205, 797
RseI CAYNNNNRTG 5 cut(s) 476, 696, 776, 935, 1407
SacI GAGCTC 2 cut(s) 471, 1296
SaqAI TTAA 4 cut(s) 51, 69, 386, 623
SatI GCNGC 3 cut(s) 186, 323, 461
Sau3AI GATC 4 cut(s) 24, 94, 199, 286
Sau96I GGNCC 5 cut(s) 494, 785, 1007, 1105, 1247
ScaI AGTACT 1 cut(s) 206
ScrFI CCNGG 5 cut(s) 195, 196, 256, 441, 868
SduI GDGCHC 5 cut(s) 117, 471, 711, 1097, 1296
SfaNI GCATC 2 cut(s) 447, 988
SfcI CTRYAG 3 cut(s) 183, 963, 980
SfoI GGCGCC 1 cut(s) 817
Sfr274I CTCGAG 1 cut(s) 464
SfuI TTCGAA 1 cut(s) 408
SinI GGWCC 2 cut(s) 1007, 1105
SlaI CTCGAG 1 cut(s) 464
SmaI CCCGGG 1 cut(s) 196
SmiMI CAYNNNNRTG 5 cut(s) 476, 696, 776, 935, 1407
SmlI CTYRAG 3 cut(s) 464, 836, 1347
SmoI CTYRAG 3 cut(s) 464, 836, 1347
SpeI ACTAGT 1 cut(s) 1439
Sse9I AATT 7 cut(s) 43, 55, 393, 830, 859, 911, 1336
SsiI CCGC 6 cut(s) 165, 416, 506, 694, 949, 1245
SspDI GGCGCC 1 cut(s) 815
SspMI CTAG 6 cut(s) 354, 566, 926, 1302, 1380, 1440
SstI GAGCTC 2 cut(s) 471, 1296
StyD4I CCNGG 5 cut(s) 193, 194, 254, 439, 866
StyI CCWWGG 1 cut(s) 531
TaaI ACNGT 3 cut(s) 16, 964, 1109
TaiI ACGT 1 cut(s) 798
TaqI TCGA 9 cut(s) 135, 408, 465, 542, 681, 717, 723, 1035, 1340
TasI AATT 7 cut(s) 43, 55, 393, 830, 859, 911, 1336
TatI WGTACW 1 cut(s) 204
TfiI GAWTC 2 cut(s) 1053, 1148
Tru1I TTAA 4 cut(s) 51, 69, 386, 623
Tru9I TTAA 4 cut(s) 51, 69, 386, 623
TscAI CASTG 5 cut(s) 110, 187, 314, 975, 1177
TseFI GTSAC 4 cut(s) 219, 698, 917, 1057
TseI GCWGC 3 cut(s) 185, 322, 460
Tsp45I GTSAC 4 cut(s) 219, 698, 917, 1057
TspDTI ATGAA 8 cut(s) 51, 199, 593, 631, 884, 1040, 1303, 1470
TspGWI ACGGA 3 cut(s) 55, 418, 1275
TspMI CCCGGG 1 cut(s) 194
TspRI CASTG 5 cut(s) 110, 187, 314, 975, 1177
VneI GTGCAC 1 cut(s) 707
VpaK11BI GGWCC 2 cut(s) 1007, 1105
XapI RAATTY 3 cut(s) 859, 911, 1336
XceI RCATGY 2 cut(s) 1081, 1372
XhoI CTCGAG 1 cut(s) 464
XmaI CCCGGG 1 cut(s) 194
XmnI GAANNNNTTC 1 cut(s) 687
XspI CTAG 6 cut(s) 354, 566, 926, 1302, 1380, 1440
ZrmI AGTACT 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.