MD10G1181000.v1.1

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0016262
Physical Location & Seq
Reverse (-)
27411839 .. 27413593
1755 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1181000.v1.1.491

Sequence Viewer

Length: 1545 bp
ATGATAGCAGAATTTGAAATGGCTCACGGGACTGTCAACATAATCCAATCCCACGAACCGTGGATTAATTATCCCAACCCTAATGTTACTGGCTTCCAAAATAATCCCATCCCCAATCCCAAGCCCGTCGAAAACCGGTTTATAAACTTGGAGAGGAGTGAACTCTCTCAGTGGGTGGAGCACATAACGAAACACCTCATCGACGACTTGCCTGAACCCGCCACCACCAACACGGCCTCCACCGACACGTTACAGACGTCTGAGGACTATTTTGTCCCGTCATCACTTTCCACCGATTTAATTAGTCCGAGGAAAGTCCCACGGAGAAGCTATGGTGAGCATGAGCATCATGAGCTCCAATGGGGCAATGAACTTCAACTCCATGCAGATAATAGCGACGTAAGAGCAAGAGGAGGGAATAATATGAGTAAGCTCGATGAGCATGGCTTAACCCTAATCACCCTCCTCTTCGAATGTGCTGTTGCCATCTCCGTTGACAACCTCCCCGAGGCCCATCGGATGCTCCTTGAGCTAACCCATATGGCCTCGCCGTATGGGCCATCATGCGCTGAGCGCGTGGTGGCTTATTTCGCCAAGGCCATGGCTAGTAGGGTTATCAGCTCCTGGTTAGGCATTTGCTCTCCATTGATCAACTACAAAAGCGTTCATAGTGCTTTTCAAGTCTTCAATACCATCTCTCCTTTCATCAAATTTGCCCACTTCACTTCCAACCAAGCCATCCTCGAGGCCTTTCACCACCGCGATAGGGTGCACATTCTTGACCTCGACATCATGCAGGGTTTGCAATGGCCAGCCTTGTTTCACATTCTCGCCACACGCATGGAGGGTCCTCCACACCTGAGAATGACCGGTGTGGGCACATCATCGGAAATTCTTTTGGAGACCGGAAAGCAGCTCTCGAATTTTGCTAAGCGGCTTGGACTATCATTCGAATTTCATCCCATTGTAAGGACAGTTGGCGAGGTAGATGCTTCCATGGTGCAAGTACAGCGAGGGGAGACCCTCGCCGTGCACTGGCTGCAACATTCGTTGTATGATGCAACCGGCTCCGACTGGAAAACGTTGAGACTCATCGAAGAACTAGCACCAAGACTTGTCACATTGGTAGAGCAAGACATGTCACATAGTGGCTCATTTTTGGACCGTTTTGTTGGGTCGTTACATTACTATTCGACAATGTTTGATTCACTAGAATCATACTTGCCCAGTGACAACCCTAGCAGGCACAAGGTGGAGCATAGTATGTTTTATAGAGAAATCAACAACATATTGGCAATTGGAGGCCCGGCAAGAAGCGGCGAGGACAAGTTCCGGCAGTGGAGGAGTGAACTTGGGGCAAGAAGTGAGTTCATGCAAGTAGAGATGAGTGGAAATTCAATGGCACAAGCACAGTTGATATTGAATATGTTTCCTCCTACACATGGGTACAGCCTTGTGCAAGGAGATGGGACTATTAGGCTTGGGTGGAAGGATACTAGTTTGTATGTTGCTTCTGCATGGACTTGCTCTCATGCTTCAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

515

Amino Acids

58.03

Weight (kDa)

5.91

Isoelectric Point (pI)

45.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 153 - 508 1.6e-110 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016262)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g21170
malus_domestica MD05G1194300.v1.1 MD10G1181000.v1.1
prunus_persica Prupe.8G220900_v2.0.a1
pyrus_communis pycom10g15680
rosa_chinensis RchiOBHm_Chr6g0287671
rosa_laevigata RLG00000012475
rosa_multiflora Rmu_sc0001813.1_g000002
rosa_roxburghii Rroxscaffold_7G00181300
rosa_rugosa Rorug06G0190500
rosa_samantha Rh6AG301000 Rh6CG302800 Rh6CG314900 Rh6DG298400
rosa_wichuraiana Rw6G025970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 143
AasI GACNNNNNNGTC 1 cut(s) 272
AatII GACGTC 1 cut(s) 260
AbsI CCTCGAGG 1 cut(s) 743
AccII CGCG 2 cut(s) 576, 762
AciI CCGC 4 cut(s) 219, 760, 934, 1317
AclI AACGTT 1 cut(s) 1082
AcoI YGGCCR 1 cut(s) 809
AcsI RAATTY 6 cut(s) 11, 710, 891, 922, 953, 1393
AcyI GRCGYC 1 cut(s) 257
AdeI CACNNNGTG 2 cut(s) 1148, 1252
AfaI GTAC 2 cut(s) 1008, 1448
AfiI CCNNNNNNNGG 5 cut(s) 508, 766, 969, 1035, 1442
AflIII ACRYGT 2 cut(s) 246, 1137
AgeI ACCGGT 2 cut(s) 135, 869
AgsI TTSAA 7 cut(s) 17, 377, 680, 688, 1398, 1423, 1539
AhlI ACTAGT 1 cut(s) 1496
AjnI CCWGG 1 cut(s) 623
AloI GAACNNNNNNTCC 2 cut(s) 363, 395
AluBI AGCT 6 cut(s) 330, 355, 433, 532, 621, 916
AluI AGCT 6 cut(s) 330, 355, 433, 532, 621, 916
Alw21I GWGCWC 4 cut(s) 183, 357, 774, 1035
Alw26I GTCTC 3 cut(s) 896, 1013, 1081
Alw44I GTGCAC 2 cut(s) 770, 1031
AlwNI CAGNNNCTG 1 cut(s) 624
Ama87I CYCGRG 2 cut(s) 506, 743
AoxI GGCC 8 cut(s) 234, 510, 543, 557, 597, 747, 809, 1303
ApaLI GTGCAC 2 cut(s) 770, 1031
ApeKI GCWGC 2 cut(s) 913, 1039
ApoI RAATTY 6 cut(s) 11, 710, 891, 922, 953, 1393
AseI ATTAAT 1 cut(s) 66
AsiGI ACCGGT 2 cut(s) 135, 869
AspLEI GCGC 2 cut(s) 569, 576
AspS9I GGNCC 5 cut(s) 511, 557, 848, 1162, 1304
AsuC2I CCSGG 1 cut(s) 1307
AsuHPI GGTGA 3 cut(s) 347, 451, 746
AsuII TTCGAA 2 cut(s) 471, 951
AvaI CYCGRG 2 cut(s) 506, 743
AvaII GGWCC 2 cut(s) 848, 1162
BaeGI GKGCMC 3 cut(s) 774, 881, 1035
BalI TGGCCA 1 cut(s) 811
BanII GRGCYC 1 cut(s) 357
BbsI GAAGAC 1 cut(s) 676
Bbv12I GWGCWC 4 cut(s) 183, 357, 774, 1035
BbvI GCAGC 2 cut(s) 925, 1026
BccI CCATC 7 cut(s) 116, 494, 522, 568, 701, 746, 1460
BceAI ACGGC 3 cut(s) 249, 535, 1013
BcgI CGANNNNNNTGC 2 cut(s) 971, 1005
BciT130I CCWGG 1 cut(s) 625
BciVI GTATCC 1 cut(s) 1486
BclI TGATCA 1 cut(s) 648
BcnI CCSGG 1 cut(s) 1307
BcoDI GTCTC 3 cut(s) 896, 1013, 1081
BcuI ACTAGT 1 cut(s) 1496
BfaI CTAG 5 cut(s) 606, 1103, 1211, 1239, 1497
BfuI GTATCC 1 cut(s) 1486
BglI GCCNNNNNGGC 1 cut(s) 556
BisI GCNGC 4 cut(s) 914, 935, 1040, 1318
BlpI GCTNAGC 2 cut(s) 570, 930
BlsI GCNGC 4 cut(s) 915, 936, 1041, 1319
Bme1390I CCNGG 2 cut(s) 625, 1307
Bme18I GGWCC 2 cut(s) 848, 1162
BmeT110I CYCGRG 2 cut(s) 506, 743
BmgT120I GGNCC 5 cut(s) 511, 557, 848, 1162, 1304
BmiI GGNNCC 2 cut(s) 849, 1069
BmrFI CCNGG 2 cut(s) 625, 1307
BmrI ACTGGG 1 cut(s) 1221
BmsI GCATC 4 cut(s) 355, 510, 979, 1048
BmuI ACTGGG 1 cut(s) 1221
BpiI GAAGAC 1 cut(s) 676
Bpu1102I GCTNAGC 2 cut(s) 570, 930
Bpu14I TTCGAA 2 cut(s) 471, 951
BpuEI CTTGAG 1 cut(s) 548
BpuMI CCSGG 1 cut(s) 1307
BsaHI GRCGYC 1 cut(s) 257
BsaI GGTCTC 2 cut(s) 896, 1013
BsaJI CCNNGG 7 cut(s) 59, 308, 320, 507, 594, 600, 996
BsaWI WCCGGW 3 cut(s) 135, 869, 905
BsaXI ACNNNNNCTCC 6 cut(s) 221, 251, 363, 393, 682, 712
Bsc4I CCNNNNNNNGG 5 cut(s) 508, 766, 969, 1035, 1442
Bse118I RCCGGY 3 cut(s) 135, 869, 1064
Bse1I ACTGG 4 cut(s) 94, 1040, 1079, 1227
Bse3DI GCAATG 2 cut(s) 373, 812
BseBI CCWGG 1 cut(s) 625
BseDI CCNNGG 7 cut(s) 59, 308, 320, 507, 594, 600, 996
BseGI GGATG 4 cut(s) 108, 525, 738, 958
BseLI CCNNNNNNNGG 5 cut(s) 508, 766, 969, 1035, 1442
BseMI GCAATG 2 cut(s) 373, 812
BseMII CTCAG 4 cut(s) 182, 252, 561, 851
BseNI ACTGG 4 cut(s) 94, 1040, 1079, 1227
BseRI GAGGAG 4 cut(s) 169, 426, 455, 1357
BseSI GKGCMC 3 cut(s) 774, 881, 1035
BseXI GCAGC 2 cut(s) 925, 1026
Bsh1236I CGCG 2 cut(s) 576, 762
BshFI GGCC 8 cut(s) 236, 512, 545, 559, 599, 749, 811, 1305
BshTI ACCGGT 2 cut(s) 135, 869
BsiHKAI GWGCWC 4 cut(s) 183, 357, 774, 1035
BsiHKCI CYCGRG 2 cut(s) 506, 743
BsiSI CCGG 6 cut(s) 136, 870, 906, 1065, 1307, 1333
BslFI GGGAC 4 cut(s) 43, 260, 302, 1483
BslI CCNNNNNNNGG 5 cut(s) 508, 766, 969, 1035, 1442
BsmAI GTCTC 3 cut(s) 896, 1013, 1081
BsmFI GGGAC 4 cut(s) 43, 260, 302, 1483
BsnI GGCC 8 cut(s) 236, 512, 545, 559, 599, 749, 811, 1305
Bso31I GGTCTC 2 cut(s) 896, 1013
BsoBI CYCGRG 2 cut(s) 506, 743
Bsp119I TTCGAA 2 cut(s) 471, 951
Bsp1286I GDGCHC 5 cut(s) 183, 357, 774, 881, 1035
Bsp143I GATC 1 cut(s) 648
Bsp1720I GCTNAGC 2 cut(s) 570, 930
Bsp19I CCATGG 2 cut(s) 600, 996
BspACI CCGC 4 cut(s) 219, 760, 934, 1317
BspANI GGCC 8 cut(s) 236, 512, 545, 559, 599, 749, 811, 1305
BspCNI CTCAG 4 cut(s) 181, 253, 562, 852
BspFNI CGCG 2 cut(s) 576, 762
BspHI TCATGA 1 cut(s) 349
BspLI GGNNCC 2 cut(s) 849, 1069
BspT104I TTCGAA 2 cut(s) 471, 951
BspTNI GGTCTC 2 cut(s) 896, 1013
BsrDI GCAATG 2 cut(s) 373, 812
BsrFI RCCGGY 3 cut(s) 135, 869, 1064
BsrI ACTGG 4 cut(s) 94, 1040, 1079, 1227
BssAI RCCGGY 3 cut(s) 135, 869, 1064
BssECI CCNNGG 7 cut(s) 59, 308, 320, 507, 594, 600, 996
BssMI GATC 1 cut(s) 648
BssNI GRCGYC 1 cut(s) 257
BssT1I CCWWGG 3 cut(s) 594, 600, 996
Bst2UI CCWGG 1 cut(s) 625
Bst4CI ACNGT 5 cut(s) 34, 60, 976, 1166, 1413
Bst6I CTCTTC 1 cut(s) 473
BstACI GRCGYC 1 cut(s) 257
BstAPI GCANNNNNTGC 2 cut(s) 802, 1039
BstBI TTCGAA 2 cut(s) 471, 951
BstC8I GCNNGC 2 cut(s) 813, 1244
BstDEI CTNAG 5 cut(s) 168, 261, 570, 860, 930
BstDSI CCRYGG 4 cut(s) 59, 320, 600, 996
BstENI CCTNNNNNAGG 1 cut(s) 506
BstF5I GGATG 4 cut(s) 108, 525, 738, 958
BstFNI CGCG 2 cut(s) 576, 762
BstHHI GCGC 2 cut(s) 569, 576
BstKTI GATC 1 cut(s) 651
BstMAI GTCTC 3 cut(s) 896, 1013, 1081
BstMBI GATC 1 cut(s) 648
BstMWI GCNNNNNNNGC 9 cut(s) 352, 439, 529, 556, 573, 590, 802, 1009, 1039
BstNI CCWGG 1 cut(s) 625
BstNSI RCATGY 1 cut(s) 1141
BstSCI CCNGG 2 cut(s) 623, 1305
BstSLI GKGCMC 3 cut(s) 774, 881, 1035
BstUI CGCG 2 cut(s) 576, 762
BstV1I GCAGC 2 cut(s) 925, 1026
BstV2I GAAGAC 1 cut(s) 676
BstXI CCANNNNNNTGG 2 cut(s) 601, 841
BsuI GTATCC 1 cut(s) 1486
BsuRI GGCC 8 cut(s) 236, 512, 545, 559, 599, 749, 811, 1305
BtgI CCRYGG 4 cut(s) 59, 320, 600, 996
BtsCI GGATG 4 cut(s) 108, 525, 738, 958
BtsI GCAGTG 1 cut(s) 1343
BtsIMutI CAGTG 4 cut(s) 176, 1033, 1234, 1343
Cac8I GCNNGC 2 cut(s) 813, 1244
CaiI CAGNNNCTG 1 cut(s) 624
CciI TCATGA 1 cut(s) 349
CfoI GCGC 2 cut(s) 569, 576
Cfr10I RCCGGY 3 cut(s) 135, 869, 1064
Cfr13I GGNCC 5 cut(s) 511, 557, 848, 1162, 1304
Csp6I GTAC 2 cut(s) 1007, 1447
CspAI ACCGGT 2 cut(s) 135, 869
CviQI GTAC 2 cut(s) 1007, 1447
DdeI CTNAG 5 cut(s) 168, 261, 570, 860, 930
DpnI GATC 1 cut(s) 650
DpnII GATC 1 cut(s) 648
DraIII CACNNNGTG 2 cut(s) 1148, 1252
DrdI GACNNNNNNGTC 1 cut(s) 272
DseDI GACNNNNNNGTC 1 cut(s) 272
EaeI YGGCCR 1 cut(s) 809
Eam1104I CTCTTC 1 cut(s) 473
EarI CTCTTC 1 cut(s) 473
Ecl136II GAGCTC 1 cut(s) 355
Eco130I CCWWGG 3 cut(s) 594, 600, 996
Eco147I AGGCCT 1 cut(s) 749
Eco24I GRGCYC 1 cut(s) 357
Eco31I GGTCTC 2 cut(s) 896, 1013
Eco47I GGWCC 2 cut(s) 848, 1162
Eco53kI GAGCTC 1 cut(s) 355
Eco88I CYCGRG 2 cut(s) 506, 743
EcoICRI GAGCTC 1 cut(s) 355
EcoNI CCTNNNNNAGG 1 cut(s) 506
EcoO109I RGGNCCY 1 cut(s) 848
EcoRII CCWGG 1 cut(s) 623
EcoT14I CCWWGG 3 cut(s) 594, 600, 996
EcoT38I GRGCYC 1 cut(s) 357
ErhI CCWWGG 3 cut(s) 594, 600, 996
FaqI GGGAC 4 cut(s) 43, 260, 302, 1483
FauI CCCGC 1 cut(s) 226
FauNDI CATATG 1 cut(s) 540
FbaI TGATCA 1 cut(s) 648
Fnu4HI GCNGC 4 cut(s) 914, 935, 1040, 1318
FokI GGATG 4 cut(s) 95, 532, 725, 945
FriOI GRGCYC 1 cut(s) 357
Fsp4HI GCNGC 4 cut(s) 914, 935, 1040, 1318
FspBI CTAG 5 cut(s) 606, 1103, 1211, 1239, 1497
GlaI GCGC 2 cut(s) 568, 575
GluI GCNGC 4 cut(s) 914, 935, 1040, 1318
HaeIII GGCC 8 cut(s) 236, 512, 545, 559, 599, 749, 811, 1305
HapII CCGG 6 cut(s) 136, 870, 906, 1065, 1307, 1333
HhaI GCGC 2 cut(s) 569, 576
Hin1I GRCGYC 1 cut(s) 257
Hin6I GCGC 2 cut(s) 567, 574
HinP1I GCGC 2 cut(s) 567, 574
HincII GTYRAC 2 cut(s) 37, 496
HindII GTYRAC 2 cut(s) 37, 496
HinfI GANTC 3 cut(s) 1089, 1205, 1214
HpaII CCGG 6 cut(s) 136, 870, 906, 1065, 1307, 1333
HphI GGTGA 3 cut(s) 347, 451, 746
Hpy166II GTNNAC 6 cut(s) 37, 161, 496, 772, 1033, 1349
Hpy188I TCNGA 5 cut(s) 262, 309, 519, 889, 1072
Hpy188III TCNNGA 4 cut(s) 350, 779, 919, 1539
Hpy8I GTNNAC 6 cut(s) 37, 161, 496, 772, 1033, 1349
Hpy99I CGWCG 3 cut(s) 131, 206, 401
HpyAV CCTTC 1 cut(s) 1483
HpyCH4III ACNGT 5 cut(s) 34, 60, 976, 1166, 1413
HpyCH4IV ACGT 4 cut(s) 248, 257, 399, 1082
HpyF10VI GCNNNNNNNGC 9 cut(s) 352, 439, 529, 556, 573, 590, 802, 1009, 1039
HpyF3I CTNAG 5 cut(s) 168, 261, 570, 860, 930
HpySE526I ACGT 4 cut(s) 248, 257, 399, 1082
Hsp92I GRCGYC 1 cut(s) 257
HspAI GCGC 2 cut(s) 567, 574
Ksp22I TGATCA 1 cut(s) 648
Kzo9I GATC 1 cut(s) 648
LmnI GCTCC 6 cut(s) 178, 360, 528, 626, 1073, 1255
Lsp1109I GCAGC 2 cut(s) 925, 1026
LweI GCATC 4 cut(s) 355, 510, 979, 1048
MaeI CTAG 5 cut(s) 606, 1103, 1211, 1239, 1497
MaeII ACGT 4 cut(s) 248, 257, 399, 1082
MaeIII GTNAC 6 cut(s) 85, 249, 1117, 1140, 1179, 1229
MalI GATC 1 cut(s) 650
MboI GATC 1 cut(s) 648
MboII GAAGA 3 cut(s) 460, 676, 1109
MfeI CAATTG 1 cut(s) 1296
MhlI GDGCHC 5 cut(s) 183, 357, 774, 881, 1035
MlsI TGGCCA 1 cut(s) 811
MluCI AATT 9 cut(s) 11, 67, 300, 710, 891, 922, 953, 1296, 1393
MluNI TGGCCA 1 cut(s) 811
MlyI GAGTC 1 cut(s) 1083
MmeI TCCRAC 2 cut(s) 753, 1095
Mox20I TGGCCA 1 cut(s) 811
MscI TGGCCA 1 cut(s) 811
MseI TTAA 3 cut(s) 66, 299, 449
MslI CAYNNNNRTG 1 cut(s) 839
Msp20I TGGCCA 1 cut(s) 811
MspI CCGG 6 cut(s) 136, 870, 906, 1065, 1307, 1333
MspR9I CCNGG 2 cut(s) 625, 1307
MunI CAATTG 1 cut(s) 1296
MvaI CCWGG 1 cut(s) 625
MvnI CGCG 2 cut(s) 576, 762
MwoI GCNNNNNNNGC 9 cut(s) 352, 439, 529, 556, 573, 590, 802, 1009, 1039
NciI CCSGG 1 cut(s) 1307
NcoI CCATGG 2 cut(s) 600, 996
NdeI CATATG 1 cut(s) 540
NdeII GATC 1 cut(s) 648
NlaIV GGNNCC 2 cut(s) 849, 1069
NmuCI GTSAC 3 cut(s) 1117, 1140, 1229
NspI RCATGY 1 cut(s) 1141
NspV TTCGAA 2 cut(s) 471, 951
PaeR7I CTCGAG 1 cut(s) 743
PagI TCATGA 1 cut(s) 349
PceI AGGCCT 1 cut(s) 749
PciI ACATGT 1 cut(s) 1137
PcsI WCGNNNNNNNCGW 1 cut(s) 254
PfeI GAWTC 2 cut(s) 1205, 1214
PinAI ACCGGT 2 cut(s) 135, 869
PkrI GCNGC 4 cut(s) 915, 936, 1041, 1319
PleI GAGTC 1 cut(s) 1083
PpsI GAGTC 1 cut(s) 1083
PpuMI RGGWCCY 1 cut(s) 848
PscI ACATGT 1 cut(s) 1137
PshBI ATTAAT 1 cut(s) 66
PsiI TTATAA 1 cut(s) 143
Psp124BI GAGCTC 1 cut(s) 357
Psp1406I AACGTT 1 cut(s) 1082
Psp5II RGGWCCY 1 cut(s) 848
Psp6I CCWGG 1 cut(s) 623
PspGI CCWGG 1 cut(s) 623
PspN4I GGNNCC 2 cut(s) 849, 1069
PspPI GGNCC 5 cut(s) 511, 557, 848, 1162, 1304
PspPPI RGGWCCY 1 cut(s) 848
PspXI VCTCGAGB 1 cut(s) 743
PstNI CAGNNNCTG 1 cut(s) 624
RsaI GTAC 2 cut(s) 1008, 1448
RsaNI GTAC 2 cut(s) 1007, 1447
RseI CAYNNNNRTG 1 cut(s) 839
SacI GAGCTC 1 cut(s) 357
SaqAI TTAA 3 cut(s) 66, 299, 449
SatI GCNGC 4 cut(s) 914, 935, 1040, 1318
Sau3AI GATC 1 cut(s) 648
Sau96I GGNCC 5 cut(s) 511, 557, 848, 1162, 1304
SchI GAGTC 1 cut(s) 1083
ScrFI CCNGG 2 cut(s) 625, 1307
SduI GDGCHC 5 cut(s) 183, 357, 774, 881, 1035
SfaNI GCATC 4 cut(s) 355, 510, 979, 1048
Sfr274I CTCGAG 1 cut(s) 743
SfuI TTCGAA 2 cut(s) 471, 951
SinI GGWCC 2 cut(s) 848, 1162
SlaI CTCGAG 1 cut(s) 743
SmiMI CAYNNNNRTG 1 cut(s) 839
SmlI CTYRAG 2 cut(s) 527, 743
SmoI CTYRAG 2 cut(s) 527, 743
SpeI ACTAGT 1 cut(s) 1496
Sse9I AATT 9 cut(s) 11, 67, 300, 710, 891, 922, 953, 1296, 1393
SseBI AGGCCT 1 cut(s) 749
SsiI CCGC 4 cut(s) 219, 760, 934, 1317
SspMI CTAG 5 cut(s) 606, 1103, 1211, 1239, 1497
SstI GAGCTC 1 cut(s) 357
StuI AGGCCT 1 cut(s) 749
StyD4I CCNGG 2 cut(s) 623, 1305
StyI CCWWGG 3 cut(s) 594, 600, 996
TaaI ACNGT 5 cut(s) 34, 60, 976, 1166, 1413
TaiI ACGT 4 cut(s) 251, 260, 402, 1085
TasI AATT 9 cut(s) 11, 67, 300, 710, 891, 922, 953, 1296, 1393
TatI WGTACW 1 cut(s) 1006
TauI GCSGC 2 cut(s) 937, 1320
TfiI GAWTC 2 cut(s) 1205, 1214
Tru1I TTAA 3 cut(s) 66, 299, 449
Tru9I TTAA 3 cut(s) 66, 299, 449
TscAI CASTG 4 cut(s) 176, 1040, 1234, 1343
TseFI GTSAC 3 cut(s) 1117, 1140, 1229
TseI GCWGC 2 cut(s) 913, 1039
Tsp45I GTSAC 3 cut(s) 1117, 1140, 1229
TspDTI ATGAA 5 cut(s) 384, 656, 694, 947, 1360
TspGWI ACGGA 2 cut(s) 337, 481
TspRI CASTG 4 cut(s) 176, 1040, 1234, 1343
VneI GTGCAC 2 cut(s) 770, 1031
VpaK11BI GGWCC 2 cut(s) 848, 1162
VspI ATTAAT 1 cut(s) 66
XagI CCTNNNNNAGG 1 cut(s) 506
XapI RAATTY 6 cut(s) 11, 710, 891, 922, 953, 1393
XceI RCATGY 1 cut(s) 1141
XhoI CTCGAG 1 cut(s) 743
XspI CTAG 5 cut(s) 606, 1103, 1211, 1239, 1497
ZraI GACGTC 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.