Rorug06G0190500

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0016262
Physical Location & Seq
Reverse (-)
30538690 .. 30541190
2501 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0190500.1

Sequence Viewer

Length: 1731 bp
ATGGCCAACTTGGAGTTGTTGTTACAGAAATGCGACTCTCTCACCCACATCAAACAGCTCCAAGCCCACCTCGTAACCATTGGCCGATTCCAACTCTACCCCTCCATCACCATCAAGCTCCTCGAGCTCTGTGCTCTTCCCCCCATCGCCAATCTCCCTTACGCCACAACCCTCTTCCACCAACTCCAAAGCCCTTCCACCAGGCACTGGAACGCCGTCGTTCGAGGCCTCGCTCAGAGCCTCCAACCCACCCAAGCCGTCACATGGTACGCCACCATGTCGCGCTCTTCCCAAAAGGTCGACGCGCTCACTTGCTCCTTCGCTCTCAAAGCGTGTGCACGCGCCTTGGCGTTGTCCGAGGCCATGCAGATTCACTCTCAGCTTCTGCGTTTCGGGTTTGGACGCGATGTGTTGTTGCGAACTACTTTGCTTGATGTGTACGCGAAAGTTGGGAAGCTGGGTTTTGCGCAGAAGGTGTTCGACGAAATGCCCGAGAGGGATATAGCTTGTTGGAATGCTTTGGTTGCTGGGTTGGCTCAGGGGAGTCAACCCAGTGAGGCTTTAGCTTTGTTTGAGAGAATGACTGAGGAAGAGGGGTTAAAGCCCAATGAAGTCACTGTCCTCGGTGCGCTCTCGGCGTGTTCTCAGTTGGGTGCATTGAGAGGTGGAGAGAGAATACATACCTATATAGTGAATGCCAAGCTTGATACGCATGTCATTGTTTGCAATGCGGTTATTGATATGTATGCAAAATGCGGGTTCGTGGATAGAGCATATGAGGTGTTTGAGAATATGAGATGTGGGAAGAATTTGATAACTTGGAATACGATGATAATGGCATTTGCTATGCACGGCGATGGTTGTAAAGCGCTTGAGCTTTTTGAGAAGATGGGTAAAAATGGAGTGTGCCCTGATGCAGTGTCGTATCTTGCTGCGTTGTGTGCGTGCAATCATGCAGGACTGGTGGAAGATGGGGTTAAGTTATTTAATTCAATGGCGGGAGGTGGGATGGTGCCGAATGTTAAGCATTATGGGACTGTGGTTGATTTGTTGGGACGAGCTGGGCGACTTCAAGAGGCTTATGATATTGTGAAATCTATGCCGATGTTTCCTGATGTAGTACTGTGGCAGACTTTGCTTGGTGCCAGCAAGACCTATGGGAATGTGGAAATGGCAGAGATGGCTTCACGGAACTTGGTTGAGTTGGGGTCTGAGGGTTGTGGTGATTTCGTGTTGTTATCAAATGTTTATGCGGCTCATAAGAGATGGGATGATGTAGGCAGGGTGAGGGAAGCCATGAAGAGAAGGGATGTGAAGAAGATACCGGGTTTCGGTTACATAGAAGTAGAAGGTGTGATACACAAATTTTTGAATGGTGATCAAAACCATGTTAATAGGCATGAGATTTATTCAAAGCTAGATGAGATCAATTGCAGGATTAAAGCCTATGGATATGTTGCAAAGACAAATCATGTGTTACATGATATTGGGGAGGAGGACAAAGAAAATGCATTGAGCTACCATTGTGAGAAGTTGGCTGTGGCTTTTGGTTTGATCAGCACTGATGAGGGGACACCAATTCAGGTGATTAAGAACCTAAGAATATGTGATGATTGTCATGTCGTGATGAAACTTATTTCAGAAATATATAATAGGGAAATTATTGTAAGGGATCGAGCCCGGTTTCACAGATTTCAAGATGGACTTTGTTCTTGCAGAGATTATTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

576

Amino Acids

64.16

Weight (kDa)

7.01

Isoelectric Point (pI)

41.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 167 - 214 8.4e-06 PPR repeat family
PPR_2 PF13041 270 - 316 6.9e-10 PPR repeat family
PPR PF01535 272 - 302 2.6e-06 PPR repeat
E_motif PF20431 386 - 448 5.6e-19 E motif
DYW_deaminase PF14432 484 - 576 8.2e-39 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016262)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g21170
malus_domestica MD05G1194300.v1.1 MD10G1181000.v1.1
prunus_persica Prupe.8G220900_v2.0.a1
pyrus_communis pycom10g15680
rosa_chinensis RchiOBHm_Chr6g0287671
rosa_laevigata RLG00000012475
rosa_multiflora Rmu_sc0001813.1_g000002
rosa_roxburghii Rroxscaffold_7G00181300
rosa_rugosa Rorug06G0190500
rosa_samantha Rh6AG301000 Rh6CG302800 Rh6CG314900 Rh6DG298400
rosa_wichuraiana Rw6G025970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 468
AccB1I GGYRCC 2 cut(s) 1012, 1142
AccB7I CCANNNNNTGG 1 cut(s) 207
AccI GTMKAC 1 cut(s) 300
AccII CGCG 5 cut(s) 283, 305, 342, 405, 443
AciI CCGC 4 cut(s) 731, 756, 998, 1253
AclWI GGATC 1 cut(s) 1680
AcoI YGGCCR 2 cut(s) 3, 82
AcsI RAATTY 2 cut(s) 808, 1364
AfaI GTAC 3 cut(s) 269, 440, 1122
AfeI AGCGCT 1 cut(s) 870
AfiI CCNNNNNNNGG 3 cut(s) 207, 264, 1331
AgsI TTSAA 5 cut(s) 993, 1073, 1372, 1413, 1697
AjnI CCWGG 1 cut(s) 200
Alw21I GWGCWC 3 cut(s) 129, 136, 340
Alw44I GTGCAC 1 cut(s) 336
AlwI GGATC 1 cut(s) 1680
AlwNI CAGNNNCTG 2 cut(s) 207, 385
Ama87I CYCGRG 2 cut(s) 122, 491
Aor51HI AGCGCT 1 cut(s) 870
AoxI GGCC 4 cut(s) 3, 82, 226, 360
ApaLI GTGCAC 1 cut(s) 336
ApeKI GCWGC 1 cut(s) 932
ApoI RAATTY 2 cut(s) 808, 1364
Asp700I GAANNNNTTC 1 cut(s) 476
AspLEI GCGC 6 cut(s) 285, 307, 344, 469, 631, 871
AsuC2I CCSGG 2 cut(s) 1326, 1681
AsuHPI GGTGA 6 cut(s) 34, 100, 1235, 1297, 1388, 1597
AvaI CYCGRG 2 cut(s) 122, 491
BaeGI GKGCMC 2 cut(s) 340, 911
BaeI ACNNNNGTAYC 2 cut(s) 699, 732
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 1012, 1142
BanII GRGCYC 2 cut(s) 129, 1681
BarI GAAGNNNNNNTAC 2 cut(s) 1341, 1373
Bbv12I GWGCWC 3 cut(s) 129, 136, 340
BbvI GCAGC 1 cut(s) 919
BceAI ACGGC 3 cut(s) 200, 242, 868
BcgI CGANNNNNNTGC 2 cut(s) 113, 147
BciT130I CCWGG 1 cut(s) 202
BclI TGATCA 2 cut(s) 1378, 1554
BcnI CCSGG 2 cut(s) 1326, 1681
BfaI CTAG 1 cut(s) 1418
BfoI RGCGCY 1 cut(s) 872
BisI GCNGC 2 cut(s) 933, 1254
BlsI GCNGC 2 cut(s) 934, 1255
BmcAI AGTACT 1 cut(s) 1122
Bme1390I CCNGG 3 cut(s) 202, 1326, 1681
BmeT110I CYCGRG 2 cut(s) 122, 491
BmiI GGNNCC 2 cut(s) 1014, 1144
BmrFI CCNGG 3 cut(s) 202, 1326, 1681
BmrI ACTGGG 1 cut(s) 546
BmsI GCATC 1 cut(s) 904
BmuI ACTGGG 1 cut(s) 546
Bpu10I CCTNAGC 1 cut(s) 537
BpuEI CTTGAG 1 cut(s) 893
BpuMI CCSGG 2 cut(s) 1326, 1681
BsaJI CCNNGG 3 cut(s) 345, 357, 622
BsaXI ACNNNNNCTCC 2 cut(s) 5, 35
Bsc4I CCNNNNNNNGG 3 cut(s) 207, 264, 1331
Bse1I ACTGG 3 cut(s) 212, 552, 966
Bse3DI GCAATG 1 cut(s) 733
BseBI CCWGG 1 cut(s) 202
BseDI CCNNGG 3 cut(s) 345, 357, 622
BseGI GGATG 3 cut(s) 1014, 1276, 1315
BseLI CCNNNNNNNGG 3 cut(s) 207, 264, 1331
BseMI GCAATG 1 cut(s) 733
BseMII CTCAG 6 cut(s) 248, 392, 551, 576, 659, 1203
BseNI ACTGG 3 cut(s) 212, 552, 966
BseRI GAGGAG 2 cut(s) 110, 1508
BseSI GKGCMC 2 cut(s) 340, 911
BseXI GCAGC 1 cut(s) 919
BseYI CCCAGC 3 cut(s) 457, 527, 1061
Bsh1236I CGCG 5 cut(s) 283, 305, 342, 405, 443
BshFI GGCC 4 cut(s) 5, 84, 228, 362
BshNI GGYRCC 2 cut(s) 1012, 1142
BsiHKAI GWGCWC 3 cut(s) 129, 136, 340
BsiHKCI CYCGRG 2 cut(s) 122, 491
BsiSI CCGG 2 cut(s) 1325, 1681
BslFI GGGAC 3 cut(s) 1048, 1068, 1585
BslI CCNNNNNNNGG 3 cut(s) 207, 264, 1331
BsmFI GGGAC 3 cut(s) 1048, 1068, 1585
BsmI GAATGC 2 cut(s) 520, 700
BsnI GGCC 4 cut(s) 5, 84, 228, 362
BsoBI CYCGRG 2 cut(s) 122, 491
Bsp1286I GDGCHC 5 cut(s) 129, 136, 340, 911, 1681
Bsp143I GATC 4 cut(s) 1378, 1425, 1554, 1672
BspACI CCGC 4 cut(s) 731, 756, 998, 1253
BspANI GGCC 4 cut(s) 5, 84, 228, 362
BspCNI CTCAG 6 cut(s) 247, 391, 550, 577, 658, 1204
BspFNI CGCG 5 cut(s) 283, 305, 342, 405, 443
BspLI GGNNCC 2 cut(s) 1014, 1144
BspPI GGATC 1 cut(s) 1680
BspQI GCTCTTC 2 cut(s) 141, 292
BspT107I GGYRCC 2 cut(s) 1012, 1142
BsrDI GCAATG 1 cut(s) 733
BsrI ACTGG 3 cut(s) 212, 552, 966
BssECI CCNNGG 3 cut(s) 345, 357, 622
BssMI GATC 4 cut(s) 1378, 1425, 1554, 1672
BssT1I CCWWGG 1 cut(s) 345
Bst2UI CCWGG 1 cut(s) 202
Bst4CI ACNGT 3 cut(s) 619, 1039, 1125
Bst6I CTCTTC 5 cut(s) 141, 179, 292, 585, 1295
BstAPI GCANNNNNTGC 1 cut(s) 1135
BstC8I GCNNGC 3 cut(s) 340, 946, 1147
BstDEI CTNAG 7 cut(s) 234, 378, 537, 585, 645, 1212, 1598
BstF5I GGATG 3 cut(s) 1014, 1276, 1315
BstFNI CGCG 5 cut(s) 283, 305, 342, 405, 443
BstH2I RGCGCY 1 cut(s) 872
BstHHI GCGC 6 cut(s) 285, 307, 344, 469, 631, 871
BstKTI GATC 4 cut(s) 1381, 1428, 1557, 1675
BstMBI GATC 4 cut(s) 1378, 1425, 1554, 1672
BstMWI GCNNNNNNNGC 9 cut(s) 124, 329, 524, 533, 635, 709, 941, 1135, 1181
BstNI CCWGG 1 cut(s) 202
BstNSI RCATGY 1 cut(s) 716
BstSCI CCNGG 3 cut(s) 200, 1324, 1679
BstSLI GKGCMC 2 cut(s) 340, 911
BstUI CGCG 5 cut(s) 283, 305, 342, 405, 443
BstV1I GCAGC 1 cut(s) 919
BsuRI GGCC 4 cut(s) 5, 84, 228, 362
BtgZI GCGATG 3 cut(s) 130, 420, 870
BtsCI GGATG 3 cut(s) 1014, 1276, 1315
BtsI GCAGTG 1 cut(s) 924
BtsIMutI CAGTG 5 cut(s) 205, 559, 615, 924, 1560
Cac8I GCNNGC 3 cut(s) 340, 946, 1147
CaiI CAGNNNCTG 2 cut(s) 207, 385
CfoI GCGC 6 cut(s) 285, 307, 344, 469, 631, 871
CseI GACGC 2 cut(s) 311, 411
Csp6I GTAC 3 cut(s) 268, 439, 1121
CviQI GTAC 3 cut(s) 268, 439, 1121
DdeI CTNAG 7 cut(s) 234, 378, 537, 585, 645, 1212, 1598
DpnI GATC 4 cut(s) 1380, 1427, 1556, 1674
DpnII GATC 4 cut(s) 1378, 1425, 1554, 1672
EaeI YGGCCR 2 cut(s) 3, 82
Eam1104I CTCTTC 5 cut(s) 141, 179, 292, 585, 1295
EarI CTCTTC 5 cut(s) 141, 179, 292, 585, 1295
Ecl136II GAGCTC 1 cut(s) 127
Eco130I CCWWGG 1 cut(s) 345
Eco147I AGGCCT 1 cut(s) 228
Eco24I GRGCYC 2 cut(s) 129, 1681
Eco47III AGCGCT 1 cut(s) 870
Eco53kI GAGCTC 1 cut(s) 127
Eco88I CYCGRG 2 cut(s) 122, 491
EcoICRI GAGCTC 1 cut(s) 127
EcoRII CCWGG 1 cut(s) 200
EcoT14I CCWWGG 1 cut(s) 345
EcoT22I ATGCAT 1 cut(s) 1513
EcoT38I GRGCYC 2 cut(s) 129, 1681
ErhI CCWWGG 1 cut(s) 345
FalI AAGNNNNNCTT 2 cut(s) 1689, 1721
FaqI GGGAC 3 cut(s) 1048, 1068, 1585
FauI CCCGC 2 cut(s) 749, 991
FauNDI CATATG 1 cut(s) 775
FbaI TGATCA 2 cut(s) 1378, 1554
FblI GTMKAC 1 cut(s) 300
Fnu4HI GCNGC 2 cut(s) 933, 1254
FokI GGATG 3 cut(s) 1021, 1283, 1322
FriOI GRGCYC 2 cut(s) 129, 1681
Fsp4HI GCNGC 2 cut(s) 933, 1254
FspBI CTAG 1 cut(s) 1418
FspI TGCGCA 1 cut(s) 468
GlaI GCGC 6 cut(s) 284, 306, 343, 468, 630, 870
GluI GCNGC 2 cut(s) 933, 1254
GsaI CCCAGC 3 cut(s) 461, 531, 1065
HaeII RGCGCY 1 cut(s) 872
HaeIII GGCC 4 cut(s) 5, 84, 228, 362
HapII CCGG 2 cut(s) 1325, 1681
HgaI GACGC 2 cut(s) 311, 411
HhaI GCGC 6 cut(s) 285, 307, 344, 469, 631, 871
Hin6I GCGC 6 cut(s) 283, 305, 342, 467, 629, 869
HinP1I GCGC 6 cut(s) 283, 305, 342, 467, 629, 869
HincII GTYRAC 2 cut(s) 301, 548
HindII GTYRAC 2 cut(s) 301, 548
HindIII AAGCTT 1 cut(s) 701
HinfI GANTC 4 cut(s) 35, 87, 370, 544
HpaII CCGG 2 cut(s) 1325, 1681
HphI GGTGA 6 cut(s) 34, 100, 1235, 1297, 1388, 1597
Hpy166II GTNNAC 4 cut(s) 301, 338, 439, 548
Hpy188I TCNGA 4 cut(s) 237, 358, 1213, 1642
Hpy188III TCNNGA 4 cut(s) 1073, 1112, 1624, 1697
Hpy8I GTNNAC 4 cut(s) 301, 338, 439, 548
Hpy99I CGWCG 3 cut(s) 221, 305, 485
HpyAV CCTTC 5 cut(s) 204, 328, 466, 1299, 1343
HpyCH4III ACNGT 3 cut(s) 619, 1039, 1125
HpyF10VI GCNNNNNNNGC 9 cut(s) 124, 329, 524, 533, 635, 709, 941, 1135, 1181
HpyF3I CTNAG 7 cut(s) 234, 378, 537, 585, 645, 1212, 1598
HspAI GCGC 6 cut(s) 283, 305, 342, 467, 629, 869
Ksp22I TGATCA 2 cut(s) 1378, 1554
Kzo9I GATC 4 cut(s) 1378, 1425, 1554, 1672
LguI GCTCTTC 2 cut(s) 141, 292
LmnI GCTCC 3 cut(s) 63, 123, 320
Lsp1109I GCAGC 1 cut(s) 919
LweI GCATC 1 cut(s) 904
MaeI CTAG 1 cut(s) 1418
MaeIII GTNAC 6 cut(s) 21, 73, 259, 613, 1334, 1476
MalI GATC 4 cut(s) 1380, 1427, 1556, 1674
MboI GATC 4 cut(s) 1378, 1425, 1554, 1672
MfeI CAATTG 1 cut(s) 1429
MhlI GDGCHC 5 cut(s) 129, 136, 340, 911, 1681
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 808, 988, 1364, 1429, 1578, 1659
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 29, 553
MmeI TCCRAC 3 cut(s) 115, 268, 491
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 1513
MroXI GAANNNNTTC 1 cut(s) 476
MscI TGGCCA 1 cut(s) 5
MseI TTAA 7 cut(s) 599, 978, 987, 1023, 1392, 1440, 1590
MslI CAYNNNNRTG 1 cut(s) 855
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 1325, 1681
MspR9I CCNGG 3 cut(s) 202, 1326, 1681
MunI CAATTG 1 cut(s) 1429
Mva1269I GAATGC 2 cut(s) 520, 700
MvaI CCWGG 1 cut(s) 202
MvnI CGCG 5 cut(s) 283, 305, 342, 405, 443
MwoI GCNNNNNNNGC 9 cut(s) 124, 329, 524, 533, 635, 709, 941, 1135, 1181
NciI CCSGG 2 cut(s) 1326, 1681
NdeI CATATG 1 cut(s) 775
NdeII GATC 4 cut(s) 1378, 1425, 1554, 1672
NlaIV GGNNCC 2 cut(s) 1014, 1144
NmeAIII GCCGAG 1 cut(s) 614
NmuCI GTSAC 2 cut(s) 259, 613
NsbI TGCGCA 1 cut(s) 468
NsiI ATGCAT 1 cut(s) 1513
NspI RCATGY 1 cut(s) 716
PaeR7I CTCGAG 1 cut(s) 122
PceI AGGCCT 1 cut(s) 228
PciSI GCTCTTC 2 cut(s) 141, 292
PcsI WCGNNNNNNNCGW 2 cut(s) 489, 1063
PctI GAATGC 2 cut(s) 520, 700
PdmI GAANNNNTTC 1 cut(s) 476
PfeI GAWTC 2 cut(s) 87, 370
PflMI CCANNNNNTGG 1 cut(s) 207
PkrI GCNGC 2 cut(s) 934, 1255
PleI GAGTC 2 cut(s) 29, 552
PpsI GAGTC 2 cut(s) 29, 552
Psp124BI GAGCTC 1 cut(s) 129
Psp6I CCWGG 1 cut(s) 200
PspFI CCCAGC 3 cut(s) 457, 527, 1061
PspGI CCWGG 1 cut(s) 200
PspN4I GGNNCC 2 cut(s) 1014, 1144
PspXI VCTCGAGB 1 cut(s) 122
PstNI CAGNNNCTG 2 cut(s) 207, 385
RsaI GTAC 3 cut(s) 269, 440, 1122
RsaNI GTAC 3 cut(s) 268, 439, 1121
RseI CAYNNNNRTG 1 cut(s) 855
SacI GAGCTC 1 cut(s) 129
SalI GTCGAC 1 cut(s) 299
SapI GCTCTTC 2 cut(s) 141, 292
SaqAI TTAA 7 cut(s) 599, 978, 987, 1023, 1392, 1440, 1590
SatI GCNGC 2 cut(s) 933, 1254
Sau3AI GATC 4 cut(s) 1378, 1425, 1554, 1672
ScaI AGTACT 1 cut(s) 1122
SchI GAGTC 2 cut(s) 29, 553
ScrFI CCNGG 3 cut(s) 202, 1326, 1681
SduI GDGCHC 5 cut(s) 129, 136, 340, 911, 1681
SfaNI GCATC 1 cut(s) 904
Sfr274I CTCGAG 1 cut(s) 122
SlaI CTCGAG 1 cut(s) 122
SmiMI CAYNNNNRTG 1 cut(s) 855
SmlI CTYRAG 2 cut(s) 122, 872
SmoI CTYRAG 2 cut(s) 122, 872
Sse9I AATT 6 cut(s) 808, 988, 1364, 1429, 1578, 1659
SseBI AGGCCT 1 cut(s) 228
SsiI CCGC 4 cut(s) 731, 756, 998, 1253
SspMI CTAG 1 cut(s) 1418
SstI GAGCTC 1 cut(s) 129
StuI AGGCCT 1 cut(s) 228
StyD4I CCNGG 3 cut(s) 200, 1324, 1679
StyI CCWWGG 1 cut(s) 345
TaaI ACNGT 3 cut(s) 619, 1039, 1125
TaqI TCGA 5 cut(s) 123, 223, 300, 480, 1675
TasI AATT 6 cut(s) 808, 988, 1364, 1429, 1578, 1659
TatI WGTACW 1 cut(s) 1120
TauI GCSGC 1 cut(s) 1256
TfiI GAWTC 2 cut(s) 87, 370
Tru1I TTAA 7 cut(s) 599, 978, 987, 1023, 1392, 1440, 1590
Tru9I TTAA 7 cut(s) 599, 978, 987, 1023, 1392, 1440, 1590
TscAI CASTG 5 cut(s) 212, 559, 622, 924, 1567
TseFI GTSAC 2 cut(s) 259, 613
TseI GCWGC 1 cut(s) 932
Tsp45I GTSAC 2 cut(s) 259, 613
TspDTI ATGAA 3 cut(s) 624, 1313, 1643
TspGWI ACGGA 1 cut(s) 1204
TspRI CASTG 5 cut(s) 212, 559, 622, 924, 1567
Van91I CCANNNNNTGG 1 cut(s) 207
VneI GTGCAC 1 cut(s) 336
XapI RAATTY 2 cut(s) 808, 1364
XceI RCATGY 1 cut(s) 716
XhoI CTCGAG 1 cut(s) 122
XmiI GTMKAC 1 cut(s) 300
XmnI GAANNNNTTC 1 cut(s) 476
XspI CTAG 1 cut(s) 1418
ZrmI AGTACT 1 cut(s) 1122
Zsp2I ATGCAT 1 cut(s) 1513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.