MD05G1214400.v1.1

heat shock protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008736
Physical Location & Seq
Reverse (-)
34564584 .. 34566602
2019 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1214400.v1.1.491

Sequence Viewer

Length: 600 bp
ATGATAAATCTGTATTTCACTGGGTCATTTTTCCGAGTCAATTTACTGCTTTTGTTATATAACCCCTATTTAATTGTGTATAAGCAGGATGGTCCAGATTATTTTTCTGAAGAGTCGAGACGTATACAGTGCACTAAGTGTGGAAATTCGCATATATGGATATGCACAAACAGAAGCAAGGCCAAGGCTAGATGGTGTCAGGATTGCTGTCAATATCACCAAGCAAAGGATGGAGATGGATGGGTTGAGTACAAAGGAACTTTAGTATTTAATAGGCCACATAAGGTGGAAATACCACGGGCCTTCGTCTGTGCTGAGAGCAAAATCTTTGATGTATCTGAATGGGCTATTTGTCAGGGAATGGCGTGCAGGCCCAACACTCATAGGCCTAGCTTCCACGTTAACATGGTTGGTTTGGAGAAAACTCAAAGATCCAACTCAAGTAGATTCCCGTGGGATTTGGATGCTGAAATGATGGATGAAGATGAAGAAGAATTTGAGGTGTGGCTTCAGCAAGCTCTGGCCTCTGGCCTCTTTTTTGTGAGTCCTCCTCTAAACGCCGAAAGAGCTGGAGTCCATTCAAGTTGCCCCAGAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ Jiv90

Protein Analysis

200

Amino Acids

23.11

Weight (kDa)

6.08

Isoelectric Point (pI)

52.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Jiv90 PF14901 38 - 135 1e-36 Cleavage inducing molecular chaperone
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 124
AclWI GGATC 1 cut(s) 426
AcsI RAATTY 2 cut(s) 145, 494
AcuI CTGAAG 2 cut(s) 129, 494
AdeI CACNNNGTG 1 cut(s) 138
AfaI GTAC 1 cut(s) 251
AfiI CCNNNNNNNGG 1 cut(s) 226
AgsI TTSAA 1 cut(s) 582
AluBI AGCT 3 cut(s) 393, 518, 569
AluI AGCT 3 cut(s) 393, 518, 569
Alw21I GWGCWC 1 cut(s) 134
Alw26I GTCTC 1 cut(s) 112
Alw44I GTGCAC 1 cut(s) 130
AlwI GGATC 1 cut(s) 426
AoxI GGCC 7 cut(s) 180, 275, 300, 371, 386, 522, 529
ApaLI GTGCAC 1 cut(s) 130
ApoI RAATTY 2 cut(s) 145, 494
AspS9I GGNCC 3 cut(s) 92, 300, 372
AsuHPI GGTGA 1 cut(s) 209
AvaII GGWCC 1 cut(s) 92
BaeGI GKGCMC 1 cut(s) 134
Bbv12I GWGCWC 1 cut(s) 134
BccI CCATC 6 cut(s) 83, 186, 224, 230, 234, 469
BcoDI GTCTC 1 cut(s) 112
BfaI CTAG 2 cut(s) 189, 390
Bme18I GGWCC 1 cut(s) 92
BmgT120I GGNCC 3 cut(s) 92, 300, 372
BmrI ACTGGG 1 cut(s) 30
BmsI GCATC 1 cut(s) 454
BmuI ACTGGG 1 cut(s) 30
BplI GAGNNNNNCTC 2 cut(s) 535, 567
BpmI CTGGAG 1 cut(s) 591
BpuEI CTTGAG 1 cut(s) 424
BsaJI CCNNGG 3 cut(s) 183, 296, 452
Bsc4I CCNNNNNNNGG 1 cut(s) 226
Bse1I ACTGG 1 cut(s) 25
BseDI CCNNGG 3 cut(s) 183, 296, 452
BseGI GGATG 5 cut(s) 94, 235, 245, 469, 484
BseLI CCNNNNNNNGG 1 cut(s) 226
BseMII CTCAG 1 cut(s) 306
BseNI ACTGG 1 cut(s) 25
BseRI GAGGAG 1 cut(s) 540
BseSI GKGCMC 1 cut(s) 134
BsgI GTGCAG 1 cut(s) 388
BshFI GGCC 7 cut(s) 182, 277, 302, 373, 388, 524, 531
BsiHKAI GWGCWC 1 cut(s) 134
BslI CCNNNNNNNGG 1 cut(s) 226
BsmAI GTCTC 1 cut(s) 112
BsmBI CGTCTC 1 cut(s) 112
BsnI GGCC 7 cut(s) 182, 277, 302, 373, 388, 524, 531
Bsp1286I GDGCHC 1 cut(s) 134
Bsp143I GATC 1 cut(s) 431
BspANI GGCC 7 cut(s) 182, 277, 302, 373, 388, 524, 531
BspCNI CTCAG 1 cut(s) 307
BspPI GGATC 1 cut(s) 426
BsrI ACTGG 1 cut(s) 25
BssECI CCNNGG 3 cut(s) 183, 296, 452
BssMI GATC 1 cut(s) 431
BssNAI GTATAC 1 cut(s) 125
BssT1I CCWWGG 1 cut(s) 183
Bst1107I GTATAC 1 cut(s) 125
Bst4CI ACNGT 1 cut(s) 129
Bst6I CTCTTC 1 cut(s) 105
BstC8I GCNNGC 3 cut(s) 367, 371, 516
BstDEI CTNAG 2 cut(s) 135, 315
BstDSI CCRYGG 2 cut(s) 296, 452
BstF5I GGATG 5 cut(s) 94, 235, 245, 469, 484
BstKTI GATC 1 cut(s) 434
BstMAI GTCTC 1 cut(s) 112
BstMBI GATC 1 cut(s) 431
BstMWI GCNNNNNNNGC 1 cut(s) 566
BstSLI GKGCMC 1 cut(s) 134
BstX2I RGATCY 1 cut(s) 431
BstYI RGATCY 1 cut(s) 431
BstZ17I GTATAC 1 cut(s) 125
BsuRI GGCC 7 cut(s) 182, 277, 302, 373, 388, 524, 531
BtgI CCRYGG 2 cut(s) 296, 452
BtsCI GGATG 5 cut(s) 94, 235, 245, 469, 484
BtsIMutI CAGTG 2 cut(s) 18, 134
Cac8I GCNNGC 3 cut(s) 367, 371, 516
Cfr13I GGNCC 3 cut(s) 92, 300, 372
Csp6I GTAC 1 cut(s) 250
CviAII CATG 1 cut(s) 406
CviQI GTAC 1 cut(s) 250
DdeI CTNAG 2 cut(s) 135, 315
DpnI GATC 1 cut(s) 433
DpnII GATC 1 cut(s) 431
DraIII CACNNNGTG 1 cut(s) 138
Eam1104I CTCTTC 1 cut(s) 105
EarI CTCTTC 1 cut(s) 105
Eco130I CCWWGG 1 cut(s) 183
Eco147I AGGCCT 1 cut(s) 388
Eco47I GGWCC 1 cut(s) 92
Eco57I CTGAAG 2 cut(s) 129, 494
EcoT14I CCWWGG 1 cut(s) 183
ErhI CCWWGG 1 cut(s) 183
Esp3I CGTCTC 1 cut(s) 112
FaeI CATG 1 cut(s) 409
FatI CATG 1 cut(s) 405
FblI GTMKAC 1 cut(s) 124
FokI GGATG 5 cut(s) 101, 242, 252, 476, 491
FspBI CTAG 2 cut(s) 189, 390
GsuI CTGGAG 1 cut(s) 591
HaeIII GGCC 7 cut(s) 182, 277, 302, 373, 388, 524, 531
Hin1II CATG 1 cut(s) 409
HincII GTYRAC 1 cut(s) 403
HindII GTYRAC 1 cut(s) 403
HinfI GANTC 5 cut(s) 36, 113, 447, 544, 573
HpaI GTTAAC 1 cut(s) 403
HphI GGTGA 1 cut(s) 209
Hpy166II GTNNAC 3 cut(s) 125, 132, 403
Hpy188I TCNGA 3 cut(s) 35, 109, 340
Hpy188III TCNNGA 3 cut(s) 95, 117, 200
Hpy8I GTNNAC 3 cut(s) 125, 132, 403
HpyAV CCTTC 1 cut(s) 313
HpyCH4III ACNGT 1 cut(s) 129
HpyCH4IV ACGT 2 cut(s) 121, 399
HpyCH4V TGCA 3 cut(s) 132, 165, 369
HpyF10VI GCNNNNNNNGC 1 cut(s) 566
HpyF3I CTNAG 2 cut(s) 135, 315
HpySE526I ACGT 2 cut(s) 121, 399
Hsp92II CATG 1 cut(s) 409
KspAI GTTAAC 1 cut(s) 403
Kzo9I GATC 1 cut(s) 431
LpnPI CCDG 9 cut(s) 6, 71, 108, 185, 341, 355, 506, 513, 555
LweI GCATC 1 cut(s) 454
MaeI CTAG 2 cut(s) 189, 390
MaeII ACGT 2 cut(s) 121, 399
MalI GATC 1 cut(s) 433
MboI GATC 1 cut(s) 431
MboII GAAGA 4 cut(s) 122, 494, 500, 503
MflI RGATCY 1 cut(s) 431
MhlI GDGCHC 1 cut(s) 134
MluCI AATT 4 cut(s) 40, 72, 145, 494
MlyI GAGTC 4 cut(s) 45, 122, 553, 582
MmeI TCCRAC 1 cut(s) 459
MnlI CCTC 5 cut(s) 493, 535, 542, 558, 561
MseI TTAA 3 cut(s) 71, 270, 402
MwoI GCNNNNNNNGC 1 cut(s) 566
NdeII GATC 1 cut(s) 431
NlaIII CATG 1 cut(s) 409
PceI AGGCCT 1 cut(s) 388
PfeI GAWTC 1 cut(s) 447
PleI GAGTC 4 cut(s) 44, 121, 552, 581
PpsI GAGTC 4 cut(s) 44, 121, 552, 581
PspPI GGNCC 3 cut(s) 92, 300, 372
PsuI RGATCY 1 cut(s) 431
RsaI GTAC 1 cut(s) 251
RsaNI GTAC 1 cut(s) 250
SaqAI TTAA 3 cut(s) 71, 270, 402
Sau3AI GATC 1 cut(s) 431
Sau96I GGNCC 3 cut(s) 92, 300, 372
SchI GAGTC 4 cut(s) 45, 122, 553, 582
SduI GDGCHC 1 cut(s) 134
SetI ASST 7 cut(s) 124, 288, 395, 402, 504, 520, 571
SfaNI GCATC 1 cut(s) 454
SinI GGWCC 1 cut(s) 92
SmlI CTYRAG 1 cut(s) 439
SmoI CTYRAG 1 cut(s) 439
Sse9I AATT 4 cut(s) 40, 72, 145, 494
SseBI AGGCCT 1 cut(s) 388
SspMI CTAG 2 cut(s) 189, 390
StuI AGGCCT 1 cut(s) 388
StyI CCWWGG 1 cut(s) 183
TaaI ACNGT 1 cut(s) 129
TaiI ACGT 2 cut(s) 124, 402
TaqI TCGA 1 cut(s) 116
TasI AATT 4 cut(s) 40, 72, 145, 494
TatI WGTACW 1 cut(s) 249
TfiI GAWTC 1 cut(s) 447
Tru1I TTAA 3 cut(s) 71, 270, 402
Tru9I TTAA 3 cut(s) 71, 270, 402
TscAI CASTG 2 cut(s) 25, 134
TspDTI ATGAA 2 cut(s) 495, 501
TspRI CASTG 2 cut(s) 25, 134
VneI GTGCAC 1 cut(s) 130
VpaK11BI GGWCC 1 cut(s) 92
XapI RAATTY 2 cut(s) 145, 494
XcmI CCANNNNNNNNNTGG 1 cut(s) 227
XmiI GTMKAC 1 cut(s) 124
XspI CTAG 2 cut(s) 189, 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.