RLG00000032985

Cleavage inducing molecular chaperone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0008736
Physical Location & Seq
Forward (+)
21752688 .. 21757239
4552 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032985

Sequence Viewer

Length: 1173 bp
ATGGCAACCGTATATGCTATTTATTGTGTCAAAGTACAAGTTGGATGGCTTGGGGTTCTGCTCGCAATAAACCTTGCCTTCTTCTCCAACGATGTATTAAACTATATTCTCCAATGGTGTGATAAGGTGACTGAGAGCCCACATTTTGAAGAGCAGAAACAATCAGAAGAAGTTATGGAAGATGACTTTTCTGCAGAGTGTGATTACTCTATTCCTACTGATGAACCTGAAAAGGTGCACTCATGTAAATCATCGAGCAAGCCAGCTACTTCAGCTGTCATTAATATTGAGAAAGAATCCTGTTCTAGTAGTAAGGTGGTCAAAGAGGAAACAAGTTCAGCTGATGAGATGCAAAAAATACTAAGCAGTATTAATCATTATGAAGCACTGGGATTTCCTCGCCACCAGAAAATTGATGCTGCAATTTTGAAAAAGGAATACCGGAAGAAGGCCATGCTTGTGCATCCTGATAAAAATATGGGAAGTGCACTAGCAAGTGAGTCATTTAAGAGACTTCAATGTGCATATGAGGTTCTTTCGGATTCCACAAAGAAAAGAGATTATGATGAGCAATTGCGGAAGGAAGAATCAAAGACTAAGAGTGTGTGCCAGACCTCTTATGCTACTTCAGCTCAGGACGGTCCAGGTTATTGTTCTGAAGAGTCGCGACGTATACAATGCACCAAGTGTGGACATTCTCATATATGGGTGTGCACCAATAGAAGTAAGACCAAGGCTAGATGGTGTCAGGATTGCTGCCAATATCATCAAGCAAAGGATGGAGATGGATGGGTCGAGTACAAAGGATCCATAGTCTTTGATAGGCCTCAGAAGGTGGAAATACCGCGAGCTTTTGTTTGTGCTGAGAGCAAAATCTTTGATGTGTCGGAATGGGCAATTTGTCAGGGAATGGCATGTAGGCCCAACACCCATCGGCCTAGTTTCCATGTAAACATGGTTGGTTTGGAGAAAACTCAAAGATCCCACTCAAGTAGATTCCCATGGGATTTGGATGCTGAAATGATGGATGAAGATGAAGAAGAATTTGAGGTGTGGCTTCAGCAAGCTCTGGCCTCGGGTCTCTTTTGTGAGTCCTCAAAACGCCGAAAAAGTTGGACTTCATTCAAGTTTCATCAATTGGGGAAGAAGCAATGGCGAAGAACTTCATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ Jiv90

Protein Analysis

391

Amino Acids

44.77

Weight (kDa)

6.48

Isoelectric Point (pI)

59.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 125 - 189 2.8e-18 DnaJ domain
Jiv90 PF14901 221 - 318 3.6e-35 Cleavage inducing molecular chaperone
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 673
AccII CGCG 2 cut(s) 667, 847
AciI CCGC 2 cut(s) 577, 845
AclWI GGATC 3 cut(s) 801, 814, 975
AcsI RAATTY 1 cut(s) 1043
AcuI CTGAAG 4 cut(s) 255, 612, 678, 1043
AdeI CACNNNGTG 1 cut(s) 687
AfaI GTAC 2 cut(s) 36, 800
AfiI CCNNNNNNNGG 1 cut(s) 448
AgsI TTSAA 4 cut(s) 149, 430, 518, 1126
AjnI CCWGG 1 cut(s) 643
AluBI AGCT 6 cut(s) 266, 275, 341, 632, 851, 1067
AluI AGCT 6 cut(s) 266, 275, 341, 632, 851, 1067
Alw21I GWGCWC 3 cut(s) 240, 490, 716
Alw26I GTCTC 2 cut(s) 505, 1085
Alw44I GTGCAC 3 cut(s) 236, 486, 712
AlwI GGATC 3 cut(s) 801, 814, 975
Ama87I CYCGRG 1 cut(s) 1075
AoxI GGCC 5 cut(s) 450, 824, 920, 935, 1071
ApaLI GTGCAC 3 cut(s) 236, 486, 712
ApeKI GCWGC 2 cut(s) 419, 756
ApoI RAATTY 1 cut(s) 1043
AseI ATTAAT 2 cut(s) 282, 372
Asp700I GAANNNNTTC 1 cut(s) 1162
AspS9I GGNCC 2 cut(s) 641, 921
AsuHPI GGTGA 1 cut(s) 139
AvaI CYCGRG 1 cut(s) 1075
AvaII GGWCC 1 cut(s) 641
BaeGI GKGCMC 3 cut(s) 240, 490, 716
BamHI GGATCC 1 cut(s) 806
BanII GRGCYC 1 cut(s) 140
Bbv12I GWGCWC 3 cut(s) 240, 490, 716
BbvI GCAGC 2 cut(s) 406, 743
BccI CCATC 7 cut(s) 39, 735, 773, 779, 783, 939, 1018
BciT130I CCWGG 1 cut(s) 645
BcoDI GTCTC 2 cut(s) 505, 1085
BfaI CTAG 4 cut(s) 306, 491, 738, 939
BfmI CTRYAG 1 cut(s) 192
BisI GCNGC 2 cut(s) 420, 757
BlsI GCNGC 2 cut(s) 421, 758
Bme1390I CCNGG 1 cut(s) 645
Bme18I GGWCC 1 cut(s) 641
BmeT110I CYCGRG 1 cut(s) 1075
BmgT120I GGNCC 2 cut(s) 641, 921
BmiI GGNNCC 1 cut(s) 808
BmrFI CCNGG 1 cut(s) 645
BmrI ACTGGG 1 cut(s) 398
BmsI GCATC 4 cut(s) 339, 406, 472, 1003
BmuI ACTGGG 1 cut(s) 398
Bpu10I CCTNAGC 1 cut(s) 633
BpuEI CTTGAG 1 cut(s) 973
BsaI GGTCTC 1 cut(s) 1085
BsaJI CCNNGG 3 cut(s) 732, 1001, 1074
BsaWI WCCGGW 1 cut(s) 441
Bsc4I CCNNNNNNNGG 1 cut(s) 448
Bse1I ACTGG 1 cut(s) 393
Bse3DI GCAATG 1 cut(s) 1157
BseBI CCWGG 1 cut(s) 645
BseDI CCNNGG 3 cut(s) 732, 1001, 1074
BseGI GGATG 6 cut(s) 50, 463, 784, 794, 1018, 1033
BseLI CCNNNNNNNGG 1 cut(s) 448
BseMI GCAATG 1 cut(s) 1157
BseMII CTCAG 4 cut(s) 123, 647, 842, 855
BseNI ACTGG 1 cut(s) 393
BseSI GKGCMC 3 cut(s) 240, 490, 716
BseXI GCAGC 2 cut(s) 406, 743
Bsh1236I CGCG 2 cut(s) 667, 847
BshFI GGCC 5 cut(s) 452, 826, 922, 937, 1073
BsiHKAI GWGCWC 3 cut(s) 240, 490, 716
BsiHKCI CYCGRG 1 cut(s) 1075
BsiSI CCGG 1 cut(s) 442
BslI CCNNNNNNNGG 1 cut(s) 448
BsmAI GTCTC 2 cut(s) 505, 1085
BsnI GGCC 5 cut(s) 452, 826, 922, 937, 1073
Bso31I GGTCTC 1 cut(s) 1085
BsoBI CYCGRG 1 cut(s) 1075
Bsp1286I GDGCHC 4 cut(s) 140, 240, 490, 716
Bsp143I GATC 2 cut(s) 806, 980
Bsp19I CCATGG 1 cut(s) 1001
Bsp68I TCGCGA 1 cut(s) 667
BspACI CCGC 2 cut(s) 577, 845
BspANI GGCC 5 cut(s) 452, 826, 922, 937, 1073
BspCNI CTCAG 4 cut(s) 124, 646, 841, 856
BspFNI CGCG 2 cut(s) 667, 847
BspLI GGNNCC 1 cut(s) 808
BspMAI CTGCAG 1 cut(s) 196
BspPI GGATC 3 cut(s) 801, 814, 975
BspQI GCTCTTC 1 cut(s) 144
BspTNI GGTCTC 1 cut(s) 1085
BsrDI GCAATG 1 cut(s) 1157
BsrI ACTGG 1 cut(s) 393
BssECI CCNNGG 3 cut(s) 732, 1001, 1074
BssMI GATC 2 cut(s) 806, 980
BssNAI GTATAC 1 cut(s) 674
BssT1I CCWWGG 2 cut(s) 732, 1001
Bst1107I GTATAC 1 cut(s) 674
Bst2UI CCWGG 1 cut(s) 645
Bst4CI ACNGT 2 cut(s) 10, 641
Bst6I CTCTTC 2 cut(s) 144, 654
BstC8I GCNNGC 5 cut(s) 63, 260, 264, 849, 1065
BstDEI CTNAG 6 cut(s) 132, 362, 597, 633, 828, 864
BstDSI CCRYGG 1 cut(s) 1001
BstF5I GGATG 6 cut(s) 50, 463, 784, 794, 1018, 1033
BstFNI CGCG 2 cut(s) 667, 847
BstKTI GATC 2 cut(s) 809, 983
BstMAI GTCTC 2 cut(s) 505, 1085
BstMBI GATC 2 cut(s) 806, 980
BstMWI GCNNNNNNNGC 2 cut(s) 272, 629
BstNI CCWGG 1 cut(s) 645
BstNSI RCATGY 1 cut(s) 918
BstSCI CCNGG 1 cut(s) 643
BstSFI CTRYAG 1 cut(s) 192
BstSLI GKGCMC 3 cut(s) 240, 490, 716
BstUI CGCG 2 cut(s) 667, 847
BstV1I GCAGC 2 cut(s) 406, 743
BstX2I RGATCY 2 cut(s) 806, 980
BstYI RGATCY 2 cut(s) 806, 980
BstZ17I GTATAC 1 cut(s) 674
BsuRI GGCC 5 cut(s) 452, 826, 922, 937, 1073
BtgI CCRYGG 1 cut(s) 1001
BtsCI GGATG 6 cut(s) 50, 463, 784, 794, 1018, 1033
BtsIMutI CAGTG 1 cut(s) 386
BtuMI TCGCGA 1 cut(s) 667
Cac8I GCNNGC 5 cut(s) 63, 260, 264, 849, 1065
Cfr13I GGNCC 2 cut(s) 641, 921
Csp6I GTAC 2 cut(s) 35, 799
CviAII CATG 7 cut(s) 243, 454, 915, 947, 955, 1002, 1167
CviQI GTAC 2 cut(s) 35, 799
DdeI CTNAG 6 cut(s) 132, 362, 597, 633, 828, 864
DpnI GATC 2 cut(s) 808, 982
DpnII GATC 2 cut(s) 806, 980
DraIII CACNNNGTG 1 cut(s) 687
Eam1104I CTCTTC 2 cut(s) 144, 654
EarI CTCTTC 2 cut(s) 144, 654
Eco130I CCWWGG 2 cut(s) 732, 1001
Eco147I AGGCCT 1 cut(s) 826
Eco24I GRGCYC 1 cut(s) 140
Eco31I GGTCTC 1 cut(s) 1085
Eco47I GGWCC 1 cut(s) 641
Eco57I CTGAAG 4 cut(s) 255, 612, 678, 1043
Eco88I CYCGRG 1 cut(s) 1075
EcoRII CCWGG 1 cut(s) 643
EcoT14I CCWWGG 2 cut(s) 732, 1001
EcoT38I GRGCYC 1 cut(s) 140
ErhI CCWWGG 2 cut(s) 732, 1001
FaeI CATG 7 cut(s) 246, 457, 918, 950, 958, 1005, 1170
FalI AAGNNNNNCTT 2 cut(s) 1102, 1134
FatI CATG 7 cut(s) 242, 453, 914, 946, 954, 1001, 1166
FauNDI CATATG 1 cut(s) 526
FblI GTMKAC 1 cut(s) 673
Fnu4HI GCNGC 2 cut(s) 420, 757
FokI GGATG 6 cut(s) 57, 450, 791, 801, 1025, 1040
FriOI GRGCYC 1 cut(s) 140
Fsp4HI GCNGC 2 cut(s) 420, 757
FspBI CTAG 4 cut(s) 306, 491, 738, 939
GluI GCNGC 2 cut(s) 420, 757
HaeIII GGCC 5 cut(s) 452, 826, 922, 937, 1073
HapII CCGG 1 cut(s) 442
Hin1II CATG 7 cut(s) 246, 457, 918, 950, 958, 1005, 1170
HinfI GANTC 7 cut(s) 296, 500, 542, 587, 662, 996, 1091
HpaII CCGG 1 cut(s) 442
HphI GGTGA 1 cut(s) 139
Hpy166II GTNNAC 6 cut(s) 238, 488, 674, 692, 714, 952
Hpy188I TCNGA 5 cut(s) 166, 541, 658, 831, 889
Hpy188III TCNNGA 4 cut(s) 467, 635, 666, 749
Hpy8I GTNNAC 6 cut(s) 238, 488, 674, 692, 714, 952
Hpy99I CGWCG 1 cut(s) 672
HpyAV CCTTC 4 cut(s) 88, 442, 574, 826
HpyCH4III ACNGT 2 cut(s) 10, 641
HpyCH4IV ACGT 1 cut(s) 670
HpyCH4V TGCA 9 cut(s) 194, 238, 352, 422, 463, 488, 524, 681, 714
HpyF10VI GCNNNNNNNGC 2 cut(s) 272, 629
HpyF3I CTNAG 6 cut(s) 132, 362, 597, 633, 828, 864
HpySE526I ACGT 1 cut(s) 670
Hsp92II CATG 7 cut(s) 246, 457, 918, 950, 958, 1005, 1170
Kzo9I GATC 2 cut(s) 806, 980
LguI GCTCTTC 1 cut(s) 144
Lsp1109I GCAGC 2 cut(s) 406, 743
LweI GCATC 4 cut(s) 339, 406, 472, 1003
MaeI CTAG 4 cut(s) 306, 491, 738, 939
MaeII ACGT 1 cut(s) 670
MaeIII GTNAC 1 cut(s) 127
MalI GATC 2 cut(s) 808, 982
MboI GATC 2 cut(s) 806, 980
MfeI CAATTG 2 cut(s) 572, 1136
MflI RGATCY 2 cut(s) 806, 980
MhlI GDGCHC 4 cut(s) 140, 240, 490, 716
MluCI AATT 6 cut(s) 411, 423, 572, 897, 1043, 1136
MlyI GAGTC 3 cut(s) 509, 671, 1100
MmeI TCCRAC 4 cut(s) 22, 111, 867, 1094
MnlI CCTC 8 cut(s) 319, 408, 523, 625, 837, 1042, 1084, 1105
MroXI GAANNNNTTC 1 cut(s) 1162
MseI TTAA 4 cut(s) 98, 282, 372, 507
MslI CAYNNNNRTG 1 cut(s) 458
MspA1I CMGCKG 2 cut(s) 275, 341
MspI CCGG 1 cut(s) 442
MspR9I CCNGG 1 cut(s) 645
MunI CAATTG 2 cut(s) 572, 1136
MvaI CCWGG 1 cut(s) 645
MvnI CGCG 2 cut(s) 667, 847
MwoI GCNNNNNNNGC 2 cut(s) 272, 629
NcoI CCATGG 1 cut(s) 1001
NdeI CATATG 1 cut(s) 526
NdeII GATC 2 cut(s) 806, 980
NlaIII CATG 7 cut(s) 246, 457, 918, 950, 958, 1005, 1170
NlaIV GGNNCC 1 cut(s) 808
NmuCI GTSAC 1 cut(s) 127
NruI TCGCGA 1 cut(s) 667
NspI RCATGY 1 cut(s) 918
PceI AGGCCT 1 cut(s) 826
PciSI GCTCTTC 1 cut(s) 144
PdmI GAANNNNTTC 1 cut(s) 1162
PfeI GAWTC 4 cut(s) 296, 542, 587, 996
PkrI GCNGC 2 cut(s) 421, 758
PleI GAGTC 3 cut(s) 508, 670, 1099
PpsI GAGTC 3 cut(s) 508, 670, 1099
PshBI ATTAAT 2 cut(s) 282, 372
Psp6I CCWGG 1 cut(s) 643
PspGI CCWGG 1 cut(s) 643
PspN4I GGNNCC 1 cut(s) 808
PspPI GGNCC 2 cut(s) 641, 921
PstI CTGCAG 1 cut(s) 196
PsuI RGATCY 2 cut(s) 806, 980
PvuII CAGCTG 2 cut(s) 275, 341
RruI TCGCGA 1 cut(s) 667
RsaI GTAC 2 cut(s) 36, 800
RsaNI GTAC 2 cut(s) 35, 799
RseI CAYNNNNRTG 1 cut(s) 458
SapI GCTCTTC 1 cut(s) 144
SaqAI TTAA 4 cut(s) 98, 282, 372, 507
SatI GCNGC 2 cut(s) 420, 757
Sau3AI GATC 2 cut(s) 806, 980
Sau96I GGNCC 2 cut(s) 641, 921
SchI GAGTC 3 cut(s) 509, 671, 1100
ScrFI CCNGG 1 cut(s) 645
SduI GDGCHC 4 cut(s) 140, 240, 490, 716
SfaNI GCATC 4 cut(s) 339, 406, 472, 1003
SfcI CTRYAG 1 cut(s) 192
SinI GGWCC 1 cut(s) 641
SmiMI CAYNNNNRTG 1 cut(s) 458
SmlI CTYRAG 1 cut(s) 988
SmoI CTYRAG 1 cut(s) 988
Sse9I AATT 6 cut(s) 411, 423, 572, 897, 1043, 1136
SseBI AGGCCT 1 cut(s) 826
SsiI CCGC 2 cut(s) 577, 845
SspI AATATT 1 cut(s) 286
SspMI CTAG 4 cut(s) 306, 491, 738, 939
StuI AGGCCT 1 cut(s) 826
StyD4I CCNGG 1 cut(s) 643
StyI CCWWGG 2 cut(s) 732, 1001
TaaI ACNGT 2 cut(s) 10, 641
TaiI ACGT 1 cut(s) 673
TaqI TCGA 2 cut(s) 254, 795
TasI AATT 6 cut(s) 411, 423, 572, 897, 1043, 1136
TatI WGTACW 2 cut(s) 34, 798
TfiI GAWTC 4 cut(s) 296, 542, 587, 996
Tru1I TTAA 4 cut(s) 98, 282, 372, 507
Tru9I TTAA 4 cut(s) 98, 282, 372, 507
TscAI CASTG 1 cut(s) 393
TseFI GTSAC 1 cut(s) 127
TseI GCWGC 2 cut(s) 419, 756
Tsp45I GTSAC 1 cut(s) 127
TspDTI ATGAA 7 cut(s) 237, 396, 1044, 1050, 1110, 1121, 1155
TspRI CASTG 1 cut(s) 393
VneI GTGCAC 3 cut(s) 236, 486, 712
VpaK11BI GGWCC 1 cut(s) 641
VspI ATTAAT 2 cut(s) 282, 372
XapI RAATTY 1 cut(s) 1043
XceI RCATGY 1 cut(s) 918
XmiI GTMKAC 1 cut(s) 673
XmnI GAANNNNTTC 1 cut(s) 1162
XspI CTAG 4 cut(s) 306, 491, 738, 939
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.