Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0012459

Physical Location & Seq

Genomic Coordinates Reverse (-)

34736193 .. 34737896

Gene Structure 1704 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1216400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1704 bp

ATGGCGGTTCTTGTAAATTATGTGTCTCCAATTGCACACCCATTGCCAGAAACCACCAGAAAAACTTGTGGGTTCTTCTCCCACATCAGGAATCTCCAACCCCTTTCCCTTAGCAAGGGATTTTCCAGAGTTTTGGCGGCTTCCCAGATCACGATTTCTCCGAAAGACACCGTTTTCGCCTTGCCCAACTGGAGGCATGCCAAGAATGACAGAAGAAGTAAGGAGCTCAGACTCATTGATGCCTTTGTGCATTTGGAGTCTATGGTGGGGAAGGACCAGAAGCCTGATGTGGCTCAGGCAACTCAGCTCTTGTATGACCTCTGCAAAGCGAATAAGATGCGAAAGGCGGTCAGAGTCATCGAAATGATGGTTGTTTCCGGCATTATACCTGATGCAGCTTCTTATACTTTCTTGGTGAATTACTTGTGTAAAAGAGGGAACATTGGGTATGCAATGCAGCTGGTAGAGAAGATGGAGGAGTACGGATATCCGACGAATACTGTGACCTACAATTCGCTCGTCAGAGGGCTTTGCGTTCGTGGAAACTTGAATCAGAGCTTGCAGCTTTTGGATAGACTGATACAGAAGGGTCTGGTTCCGAATGTTTATACGTACTCTTTCTTACTTGAAGCTGCTTATAAGGAAAGAGGGGTCAATGAAGCCATGAAGCTCCTGCAGGAGATAATTGCTAAAGGTGGAAAGCCTAATGTGGTCAGTTACAATGTCTTGTTAACTGGTTTGTGCAAGGAAGGTAGGACTGATGAGGCATTTCGGCTCTTTAGGAGTTTGCCTTCGATGGGGTTCGATCCAAATGTTGTGAGCTATAACATCGTACTAAGGAGTTTGTGTCACGAGGGTAGGTGGGAAGAGGCAAATGTGCTTTTGTCTGAGATGGAAGGAGAGGATCGAGCCCCTTCCATAGTTACGTATAACATCTTAATTGGTTCACTTGCACTTCATGGTAGAACTGAACATGCTCTTCAAGTTTTGGACGAAATGCAGAGAGGTCGGTTCAAGCCTACTGCTGCCAGCTACAATCCGATCATTGCTCATCTCTGCAAAGAGGGGAAAGTGGATGGTGCGGTCAAGTGTCTAGACCAAATGGTTCATCGGCGGTGTAATCCTAATGAGGGAACATTCAATGCCATTGCTGTGCTATGTGAGGAAGGTTTGGTGCCAGAGGCATTCTCTATCATTCAAAGCCTCTGCAATAAACAAAAGTGCTCCAGCTACGAGTTGTACAAAAACGTAATCACAAGCTTATGTCGGAAAGGGAACACGTTTCCAGCGTTTCAGATATTGTACGAAATGACCAAGCACGGTTTCACTCCAGATTCTTATACGTATTCATCTCTGATCAGAGGATTGTGTTTGGAGGGTATGCTGGATGAGGCCATGGAGATTTTCAAGGTAATGGAAGAAAACAGCCTTAGGCCTGATACGGACAATTTCAATGCCCTTATACTCGGTTTCTGCAAGTCTCGAAGAACAGACTTGTCCTTCCAGGTTTTTGAGATGATGATAGAGAAAGGGCGTATGCCTAACGAAATGACCTACACCATTCTTGTGGAAGGGATTGCGCACGAAGGAGAACTGGAACTGGCAGCCAGAGTCTTAAAAGAGTTGCACCTGAGACATGTAGTGAGTCCGAATACAGTGGAAAGACTGGTTATGCAGTACGACTTTGAGGATTTACCGGGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

568

Amino Acids

64.02

Weight (kDa)

7.51

Isoelectric Point (pI)

37.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	101 - 144	9.8e-08	PPR repeat family
PPR_long	PF17177	110 - 213	8.8e-07	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	127 - 159	7e-07	PPR repeat
PPR_2	PF13041	130 - 178	5.6e-12	PPR repeat family
TPR_24	PF23276	146 - 262	2.8e-07	Fungal tetratrico peptide repeats
PPR_3	PF13812	154 - 211	2.9e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	163 - 192	4.9e-06	PPR repeat
PPR_2	PF13041	166 - 211	4.2e-11	PPR repeat family
PPR_2	PF13041	208 - 249	4.1e-08	PPR repeat family
PPR_3	PF13812	223 - 280	1.3e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	231 - 261	5.8e-12	PPR repeat
PPR_2	PF13041	235 - 284	3.6e-17	PPR repeat family
PPR	PF01535	238 - 268	5.1e-07	PPR repeat
PPR_1	PF12854	266 - 299	7.3e-09	PPR repeat
PPR_2	PF13041	270 - 318	8.1e-14	PPR repeat family
PPR_3	PF13812	294 - 350	2e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	305 - 334	2.6e-07	PPR repeat
PPR_2	PF13041	305 - 346	3.5e-09	PPR repeat family
PPR	PF01535	308 - 335	3.7e-06	PPR repeat
PPR_1	PF12854	338 - 369	1.1e-06	PPR repeat
PPR_2	PF13041	340 - 383	4.6e-11	PPR repeat family
PPR_2	PF13041	414 - 457	1.4e-11	PPR repeat family
PPR_3	PF13812	433 - 488	6.7e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	441 - 473	3.5e-12	PPR repeat
PPR	PF01535	447 - 475	6.5e-08	PPR repeat
PPR_2	PF13041	479 - 527	3.4e-11	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012459)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79080
fragaria_vesca	FvH4_3g15990
malus_domestica	MD05G1216400.v1.1 MD10G1198700.v1.1
prunus_persica	Prupe.4G143000_v2.0.a1
pyrus_communis	pycom05g19870 pycom10g17140
rosa_chinensis	RchiOBHm_Chr5g0026921
rosa_laevigata	RLG00000032970
rosa_multiflora	Rmu_co8463079.1_g000001 Rmu_sc0004223.1_g000006 Rmu_sc0008732.1_g000003
rosa_roxburghii	Rroxscaffold_1G00052550
rosa_rugosa	Rorug05G0095900
rosa_samantha	Rh5AG189500 Rh5BG187100 Rh5CG206800 Rh5DG188800
rosa_wichuraiana	Rw5G017240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	639
Acc16I	TGCGCA	1 cut(s)	1581
AccB1I	GGYRCC	1 cut(s)	1174
AciI	CCGC	5 cut(s)	5, 137, 347, 1082, 1114
AclWI	GGATC	2 cut(s)	800, 912
AfaI	GTAC	6 cut(s)	482, 614, 834, 1241, 1304, 1679
AfiI	CCNNNNNNNGG	5 cut(s)	87, 115, 192, 797, 1130
AflIII	ACRYGT	2 cut(s)	1278, 1636
AgsI	TTSAA	8 cut(s)	550, 629, 983, 1015, 1141, 1199, 1408, 1453
AjnI	CCWGG	1 cut(s)	1503
AjuI	GAANNNNNNNTTGG	2 cut(s)	1307, 1339
Alw21I	GWGCWC	2 cut(s)	228, 1226
Alw26I	GTCTC	3 cut(s)	30, 1485, 1627
AlwI	GGATC	2 cut(s)	800, 912
AoxI	GGCC	2 cut(s)	1392, 1433
ApeKI	GCWGC	6 cut(s)	395, 457, 562, 632, 1025, 1604
ArsI	GACNNNNNNTTYG	4 cut(s)	158, 190, 496, 528
AspLEI	GCGC	1 cut(s)	1582
AspS9I	GGNCC	1 cut(s)	274
AsuC2I	CCSGG	1 cut(s)	1698
AsuHPI	GGTGA	1 cut(s)	427
AvaII	GGWCC	1 cut(s)	274
AxyI	CCTNAGG	1 cut(s)	1430
BanI	GGYRCC	1 cut(s)	1174
BanII	GRGCYC	2 cut(s)	228, 913
BauI	CACGAG	1 cut(s)	851
Bbv12I	GWGCWC	2 cut(s)	228, 1226
BbvI	GCAGC	6 cut(s)	407, 469, 574, 619, 1012, 1616
BccI	CCATC	5 cut(s)	361, 466, 790, 886, 1070
BcgI	CGANNNNNNTGC	4 cut(s)	319, 353, 989, 1023
BciT130I	CCWGG	1 cut(s)	1505
BclI	TGATCA	1 cut(s)	1356
BcnI	CCSGG	1 cut(s)	1698
BcoDI	GTCTC	3 cut(s)	30, 1485, 1627
BfaI	CTAG	1 cut(s)	1094
BfmI	CTRYAG	1 cut(s)	674
BisI	GCNGC	7 cut(s)	138, 396, 458, 563, 633, 1026, 1605
BlsI	GCNGC	7 cut(s)	139, 397, 459, 564, 634, 1027, 1606
Bme1390I	CCNGG	2 cut(s)	1505, 1698
Bme18I	GGWCC	1 cut(s)	274
BmgT120I	GGNCC	1 cut(s)	274
BmiI	GGNNCC	2 cut(s)	597, 1176
BmrFI	CCNGG	2 cut(s)	1505, 1698
BmsI	GCATC	3 cut(s)	229, 327, 382
BplI	GAGNNNNNCTC	2 cut(s)	1172, 1204
BpmI	CTGGAG	3 cut(s)	211, 1210, 1314
Bpu10I	CCTNAGC	2 cut(s)	110, 294
BpuMI	CCSGG	1 cut(s)	1698
BsaAI	YACGTR	3 cut(s)	612, 927, 1344
BsaJI	CCNNGG	1 cut(s)	1395
BsaXI	ACNNNNNCTCC	4 cut(s)	142, 172, 832, 862
Bsc4I	CCNNNNNNNGG	5 cut(s)	87, 115, 192, 797, 1130
Bse1I	ACTGG	5 cut(s)	194, 739, 1599, 1605, 1671
Bse21I	CCTNAGG	1 cut(s)	1430
Bse3DI	GCAATG	4 cut(s)	41, 459, 1044, 1146
BseBI	CCWGG	1 cut(s)	1505
BseDI	CCNNGG	1 cut(s)	1395
BseGI	GGATG	2 cut(s)	1081, 1393
BseLI	CCNNNNNNNGG	5 cut(s)	87, 115, 192, 797, 1130
BseMI	GCAATG	4 cut(s)	41, 459, 1044, 1146
BseMII	CTCAG	5 cut(s)	241, 308, 317, 879, 1622
BseNI	ACTGG	5 cut(s)	194, 739, 1599, 1605, 1671
BseRI	GAGGAG	1 cut(s)	491
BseXI	GCAGC	6 cut(s)	407, 469, 574, 619, 1012, 1616
BshFI	GGCC	2 cut(s)	1394, 1435
BshNI	GGYRCC	1 cut(s)	1174
BsiHKAI	GWGCWC	2 cut(s)	228, 1226
BsiSI	CCGG	2 cut(s)	378, 1697
BslI	CCNNNNNNNGG	5 cut(s)	87, 115, 192, 797, 1130
BsmAI	GTCTC	3 cut(s)	30, 1485, 1627
BsmI	GAATGC	1 cut(s)	1184
BsnI	GGCC	2 cut(s)	1394, 1435
Bsp1286I	GDGCHC	3 cut(s)	228, 913, 1226
Bsp1407I	TGTACA	1 cut(s)	1239
Bsp143I	GATC	5 cut(s)	147, 805, 904, 1041, 1356
Bsp19I	CCATGG	1 cut(s)	1395
BspACI	CCGC	5 cut(s)	5, 137, 347, 1082, 1114
BspANI	GGCC	2 cut(s)	1394, 1435
BspCNI	CTCAG	5 cut(s)	240, 307, 316, 880, 1623
BspLI	GGNNCC	2 cut(s)	597, 1176
BspMAI	CTGCAG	1 cut(s)	678
BspPI	GGATC	2 cut(s)	800, 912
BspQI	GCTCTTC	1 cut(s)	984
BspT107I	GGYRCC	1 cut(s)	1174
BsrDI	GCAATG	4 cut(s)	41, 459, 1044, 1146
BsrGI	TGTACA	1 cut(s)	1239
BsrI	ACTGG	5 cut(s)	194, 739, 1599, 1605, 1671
BssECI	CCNNGG	1 cut(s)	1395
BssMI	GATC	5 cut(s)	147, 805, 904, 1041, 1356
BssSI	CACGAG	1 cut(s)	851
BssT1I	CCWWGG	1 cut(s)	1395
Bst2BI	CACGAG	1 cut(s)	851
Bst2UI	CCWGG	1 cut(s)	1505
Bst4CI	ACNGT	4 cut(s)	172, 502, 1322, 1657
Bst6I	CTCTTC	2 cut(s)	861, 984
BstAUI	TGTACA	1 cut(s)	1239
BstBAI	YACGTR	3 cut(s)	612, 927, 1344
BstC8I	GCNNGC	3 cut(s)	198, 560, 1030
BstDEI	CTNAG	8 cut(s)	110, 227, 294, 303, 836, 888, 1430, 1631
BstDSI	CCRYGG	1 cut(s)	1395
BstENI	CCTNNNNNAGG	1 cut(s)	113
BstF5I	GGATG	2 cut(s)	1081, 1393
BstHHI	GCGC	1 cut(s)	1582
BstKTI	GATC	5 cut(s)	150, 808, 907, 1044, 1359
BstMAI	GTCTC	3 cut(s)	30, 1485, 1627
BstMBI	GATC	5 cut(s)	147, 805, 904, 1041, 1356
BstNI	CCWGG	1 cut(s)	1505
BstNSI	RCATGY	3 cut(s)	200, 977, 1640
BstSCI	CCNGG	2 cut(s)	1503, 1696
BstSFI	CTRYAG	1 cut(s)	674
BstSNI	TACGTA	3 cut(s)	612, 927, 1344
BstV1I	GCAGC	6 cut(s)	407, 469, 574, 619, 1012, 1616
BstXI	CCANNNNNNTGG	2 cut(s)	133, 1567
Bsu36I	CCTNAGG	1 cut(s)	1430
BsuRI	GGCC	2 cut(s)	1394, 1435
BtgI	CCRYGG	1 cut(s)	1395
BtsCI	GGATG	2 cut(s)	1081, 1393
BtsIMutI	CAGTG	1 cut(s)	1662
Cac8I	GCNNGC	3 cut(s)	198, 560, 1030
CfoI	GCGC	1 cut(s)	1582
Cfr13I	GGNCC	1 cut(s)	274
Csp6I	GTAC	6 cut(s)	481, 613, 833, 1240, 1303, 1678
CviAII	CATG	6 cut(s)	197, 664, 959, 974, 1396, 1637
CviQI	GTAC	6 cut(s)	481, 613, 833, 1240, 1303, 1678
DdeI	CTNAG	8 cut(s)	110, 227, 294, 303, 836, 888, 1430, 1631
DpnI	GATC	5 cut(s)	149, 807, 906, 1043, 1358
DpnII	GATC	5 cut(s)	147, 805, 904, 1041, 1356
Eam1104I	CTCTTC	2 cut(s)	861, 984
EarI	CTCTTC	2 cut(s)	861, 984
Ecl136II	GAGCTC	1 cut(s)	226
Eco105I	TACGTA	3 cut(s)	612, 927, 1344
Eco130I	CCWWGG	1 cut(s)	1395
Eco147I	AGGCCT	1 cut(s)	1435
Eco24I	GRGCYC	2 cut(s)	228, 913
Eco32I	GATATC	1 cut(s)	488
Eco47I	GGWCC	1 cut(s)	274
Eco53kI	GAGCTC	1 cut(s)	226
Eco81I	CCTNAGG	1 cut(s)	1430
EcoICRI	GAGCTC	1 cut(s)	226
EcoNI	CCTNNNNNAGG	1 cut(s)	113
EcoRII	CCWGG	1 cut(s)	1503
EcoRV	GATATC	1 cut(s)	488
EcoT14I	CCWWGG	1 cut(s)	1395
EcoT38I	GRGCYC	2 cut(s)	228, 913
ErhI	CCWWGG	1 cut(s)	1395
FaeI	CATG	6 cut(s)	200, 667, 962, 977, 1399, 1640
FatI	CATG	6 cut(s)	196, 663, 958, 973, 1395, 1636
FbaI	TGATCA	1 cut(s)	1356
Fnu4HI	GCNGC	7 cut(s)	138, 396, 458, 563, 633, 1026, 1605
FokI	GGATG	2 cut(s)	1088, 1400
FriOI	GRGCYC	2 cut(s)	228, 913
Fsp4HI	GCNGC	7 cut(s)	138, 396, 458, 563, 633, 1026, 1605
FspBI	CTAG	1 cut(s)	1094
FspI	TGCGCA	1 cut(s)	1581
GlaI	GCGC	1 cut(s)	1581
GluI	GCNGC	7 cut(s)	138, 396, 458, 563, 633, 1026, 1605
GsuI	CTGGAG	3 cut(s)	211, 1210, 1314
HaeIII	GGCC	2 cut(s)	1394, 1435
HapII	CCGG	2 cut(s)	378, 1697
HhaI	GCGC	1 cut(s)	1582
Hin1II	CATG	6 cut(s)	200, 667, 962, 977, 1399, 1640
Hin6I	GCGC	1 cut(s)	1580
HinP1I	GCGC	1 cut(s)	1580
HincII	GTYRAC	1 cut(s)	732
HindII	GTYRAC	1 cut(s)	732
HindIII	AAGCTT	1 cut(s)	1258
HinfI	GANTC	8 cut(s)	91, 231, 257, 354, 550, 1334, 1611, 1645
HpaI	GTTAAC	1 cut(s)	732
HpaII	CCGG	2 cut(s)	378, 1697
HphI	GGTGA	1 cut(s)	427
Hpy166II	GTNNAC	2 cut(s)	732, 947
Hpy188III	TCNNGA	7 cut(s)	88, 126, 151, 851, 1094, 1331, 1482
Hpy8I	GTNNAC	2 cut(s)	732, 947
Hpy99I	CGWCG	1 cut(s)	496
HpyCH4III	ACNGT	4 cut(s)	172, 502, 1322, 1657
HpyCH4IV	ACGT	5 cut(s)	611, 926, 1248, 1280, 1343
HpyF3I	CTNAG	8 cut(s)	110, 227, 294, 303, 836, 888, 1430, 1631
HpySE526I	ACGT	5 cut(s)	611, 926, 1248, 1280, 1343
Hsp92II	CATG	6 cut(s)	200, 667, 962, 977, 1399, 1640
HspAI	GCGC	1 cut(s)	1580
Ksp22I	TGATCA	1 cut(s)	1356
KspAI	GTTAAC	1 cut(s)	732
Kzo9I	GATC	5 cut(s)	147, 805, 904, 1041, 1356
LguI	GCTCTTC	1 cut(s)	984
LmnI	GCTCC	3 cut(s)	223, 675, 1229
Lsp1109I	GCAGC	6 cut(s)	407, 469, 574, 619, 1012, 1616
LweI	GCATC	3 cut(s)	229, 327, 382
MaeI	CTAG	1 cut(s)	1094
MaeII	ACGT	5 cut(s)	611, 926, 1248, 1280, 1343
MaeIII	GTNAC	4 cut(s)	502, 716, 848, 922
MalI	GATC	5 cut(s)	149, 807, 906, 1043, 1358
MboI	GATC	5 cut(s)	147, 805, 904, 1041, 1356
MboII	GAAGA	7 cut(s)	67, 225, 481, 878, 971, 1430, 1497
MfeI	CAATTG	1 cut(s)	30
MhlI	GDGCHC	3 cut(s)	228, 913, 1226
MluCI	AATT	7 cut(s)	16, 30, 418, 511, 684, 939, 1447
MlyI	GAGTC	5 cut(s)	225, 266, 363, 1620, 1654
MmeI	TCCRAC	3 cut(s)	121, 515, 1247
MseI	TTAA	3 cut(s)	731, 938, 1616
MslI	CAYNNNNRTG	3 cut(s)	362, 1151, 1565
MspA1I	CMGCKG	1 cut(s)	460
MspI	CCGG	2 cut(s)	378, 1697
MspR9I	CCNGG	2 cut(s)	1505, 1698
MunI	CAATTG	1 cut(s)	30
Mva1269I	GAATGC	1 cut(s)	1184
MvaI	CCWGG	1 cut(s)	1505
NciI	CCSGG	1 cut(s)	1698
NcoI	CCATGG	1 cut(s)	1395
NdeII	GATC	5 cut(s)	147, 805, 904, 1041, 1356
NlaIII	CATG	6 cut(s)	200, 667, 962, 977, 1399, 1640
NlaIV	GGNNCC	2 cut(s)	597, 1176
NmuCI	GTSAC	2 cut(s)	502, 848
NsbI	TGCGCA	1 cut(s)	1581
NspI	RCATGY	3 cut(s)	200, 977, 1640
PaeI	GCATGC	1 cut(s)	200
PceI	AGGCCT	1 cut(s)	1435
PciI	ACATGT	1 cut(s)	1636
PciSI	GCTCTTC	1 cut(s)	984
PcsI	WCGNNNNNNNCGW	2 cut(s)	158, 1286
PctI	GAATGC	1 cut(s)	1184
PfeI	GAWTC	3 cut(s)	91, 550, 1334
PkrI	GCNGC	7 cut(s)	139, 397, 459, 564, 634, 1027, 1606
PleI	GAGTC	5 cut(s)	225, 265, 362, 1619, 1653
PpsI	GAGTC	5 cut(s)	225, 265, 362, 1619, 1653
Ppu21I	YACGTR	3 cut(s)	612, 927, 1344
PscI	ACATGT	1 cut(s)	1636
PsiI	TTATAA	1 cut(s)	639
Psp124BI	GAGCTC	1 cut(s)	228
Psp6I	CCWGG	1 cut(s)	1503
PspGI	CCWGG	1 cut(s)	1503
PspN4I	GGNNCC	2 cut(s)	597, 1176
PspPI	GGNCC	1 cut(s)	274
PstI	CTGCAG	1 cut(s)	678
PvuII	CAGCTG	1 cut(s)	460
RsaI	GTAC	6 cut(s)	482, 614, 834, 1241, 1304, 1679
RsaNI	GTAC	6 cut(s)	481, 613, 833, 1240, 1303, 1678
RseI	CAYNNNNRTG	3 cut(s)	362, 1151, 1565
SacI	GAGCTC	1 cut(s)	228
SapI	GCTCTTC	1 cut(s)	984
SaqAI	TTAA	3 cut(s)	731, 938, 1616
SatI	GCNGC	7 cut(s)	138, 396, 458, 563, 633, 1026, 1605
Sau3AI	GATC	5 cut(s)	147, 805, 904, 1041, 1356
Sau96I	GGNCC	1 cut(s)	274
SbfI	CCTGCAGG	1 cut(s)	678
SchI	GAGTC	5 cut(s)	225, 266, 363, 1620, 1654
ScrFI	CCNGG	2 cut(s)	1505, 1698
SdaI	CCTGCAGG	1 cut(s)	678
SduI	GDGCHC	3 cut(s)	228, 913, 1226
SfaNI	GCATC	3 cut(s)	229, 327, 382
SfcI	CTRYAG	1 cut(s)	674
SinI	GGWCC	1 cut(s)	274
SmiMI	CAYNNNNRTG	3 cut(s)	362, 1151, 1565
SnaBI	TACGTA	3 cut(s)	612, 927, 1344
SphI	GCATGC	1 cut(s)	200
Sse8387I	CCTGCAGG	1 cut(s)	678
Sse9I	AATT	7 cut(s)	16, 30, 418, 511, 684, 939, 1447
SseBI	AGGCCT	1 cut(s)	1435
SsiI	CCGC	5 cut(s)	5, 137, 347, 1082, 1114
SspMI	CTAG	1 cut(s)	1094
SstI	GAGCTC	1 cut(s)	228
StuI	AGGCCT	1 cut(s)	1435
StyD4I	CCNGG	2 cut(s)	1503, 1696
StyI	CCWWGG	1 cut(s)	1395
TaaI	ACNGT	4 cut(s)	172, 502, 1322, 1657
TaiI	ACGT	5 cut(s)	614, 929, 1251, 1283, 1346
TaqI	TCGA	5 cut(s)	360, 794, 804, 907, 1483
TasI	AATT	7 cut(s)	16, 30, 418, 511, 684, 939, 1447
TatI	WGTACW	1 cut(s)	1239
TauI	GCSGC	1 cut(s)	140
TfiI	GAWTC	3 cut(s)	91, 550, 1334
Tru1I	TTAA	3 cut(s)	731, 938, 1616
Tru9I	TTAA	3 cut(s)	731, 938, 1616
TscAI	CASTG	1 cut(s)	1662
TseFI	GTSAC	2 cut(s)	502, 848
TseI	GCWGC	6 cut(s)	395, 457, 562, 632, 1025, 1604
Tsp45I	GTSAC	2 cut(s)	502, 848
TspDTI	ATGAA	5 cut(s)	672, 680, 947, 1097, 1338
TspGWI	ACGGA	2 cut(s)	498, 1457
TspRI	CASTG	1 cut(s)	1662
VpaK11BI	GGWCC	1 cut(s)	274
XagI	CCTNNNNNAGG	1 cut(s)	113
XbaI	TCTAGA	1 cut(s)	1093
XceI	RCATGY	3 cut(s)	200, 977, 1640
XspI	CTAG	1 cut(s)	1094

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.