Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0008771

Physical Location & Seq

Genomic Coordinates Reverse (-)

37518068 .. 37522895

Gene Structure 4828 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1242400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2496 bp

ATGGAGGCTTATGGCGGATACTTGGTGGACGAGAAGGCGGTGAGGGTAGAGTCCATCTTCGTCGAGTTCCTCAAGAACTTCAGGATCCCCGGCGGAGGAGGAGAGGCGTATTACGAGGCGGAGATCGAAGCCATGGCCGCCAATGAGTCCAGCATCATGTTCATCGACTTCTCCCACGTCATGGTTTTCAACAATCTCCTCCAGAAGGCCATTTCCGACGAGTTTCTCAGGTTTGAGCCCTATTTGAAGAACGCTTGCAAGACATTCGTGACGCAGCTCCGCCCAAACTTCATCTCCGACGATACGAACAAGGACATCAATGTCGCATTCTTCAACCTCCCCGTCTCCAAGAGGTTGAGGGAATTGACCACAGCAGAAATTGGGAAATTGGTATCAGTGAAAGGAGTGGTGACTCGGACCAGTGAGGTTCGACCGGAGCTACTCCACGGGACATTCAAGTGCTTGGAGTGCGGAGGCGTCATCAAGAATGTCGAACAACAATTCAAGTACACTGAGCCAACAATCTGTGTCAATGCCACATGCTCTAATAGAGCAAGGTGGGCGTTGCTTCGGCAAGAGAGTAAATTTGCAGACTGGCAGAGGGTACGAATGCAGGAGACGTCCAAAGAGATACCTGCGGGCTCCCTTCCCAGATCACTGGATGTTATTATCCGCCATGAGATTGTTGAGAAGGCCAGGGCCGGTGATACGGTCATTTTCACAGGCACAGTGGTTGTCATACCAGACATAATGGCATTGGCCTCCCCTGGCGACAGAGCAGAGTGTTCTCGACAAGCATCCCAGCGCAATGGTTCTATGGCTGGACATGAAGGAGTGAGGGGTCTTAAAGCATTGGGAGTGAGAGACCTCTCCTATCGCCTTGCCTTTATTGCAAATTCAGTTCAGATTTCTGATGGTAGACAGGATACTGATGTCCGGAATAGAAAGAAGGATGCTGACGACGATGACAACCAGCAGTTCACTGAAGAAGAGAAAGACGAAGTTCAAAGAATGAGGAATACCCCTGATTTCTTCAATAAGCTTGTTGATAGCATTGCCCCAACTGTATTTGGTCATCAAGACATCAAGCGTGCAATTCTGCTTATGCTCCTTGGTGGTGTCCACAAGTTTACTCATGAAGGCATCAACTTGAGAGGAGACATCAATGTTTGTATTGTTGGAGACCCGAGTTGCGCAAAATCCCAGTTCCTAAAGTATACGGCAGGAATAGTTCCAAGATCCGTTTACACTTCTGGGAAATCCTCCTCTGCTGCTGGTTTAACTGCAACTGTTGCCAAAGAACCAGAAACAGGAGAATTTTGTATTGAGGCAGGTGCCTTAATGCTTGCTGACAATGGTATTTGTTGCATTGATGAATTTGACAAAATGGACATTAGGGATCAGGTTGCGATTCATGAGGCAATGGAACAACAGACAATAAGCATTACAAAAGCAGGAATACAGGCAACACTGAATGCTCGAACATCAATTCTTGCTGCTGCTAACCCCACAGGCGGCCGTTATGATAAATCTAAACCACTCAAGTATAATGTGGCTCTTCCTCCACCTATCCTCTCAAGGTTTGATCTGGTATATGTTATGATTGATGACCCAGATGATCAAACTGATTACCACATTGCTCACCACATTGTAAGAGTGCATCAAAAGCGCGAAGAGGCACTCACTCCTGAATTCAGCACTGCACAGCTAAAACGTTATATTGCATTTGCTAAAACTCTGAAACCTAAGTTAAACTCAGATGCTAGGAAGCTGTTGGTGGAGTCTTATGTTGCTCTCAGAAGAGGTGATACTGCTCCTGGTAGTAGAGTTGCTTATCGCATGACAGTTAGGCAACTAGAGGCGTTGATCAGATTGTCCGAAGCCATTGCTCGAAGTCATTTAGACCTTCAGGTAAAACCAGCCCATGTCCGTTTAGCAGTGAGATTGCTGAAAACGTCAGTGATCAGGTATGAAATCTATGACACTGTTGAATATCCTCCTCTCGTATCTGTGGAGTCTAGTGAGATCGATCTGTCCGATTTTGATCAAGGTGTAGCAAATGATGATGGTGCAGCAGACCAAGGAAAGATTAAAGTAAGTGATGAATACTTTCAGAGGATTGCCCAGGCCCTTGTCTTGCGTCTTAGACAGCATGAAGAAGATGTTAGGCAAACCGGGACAGGGCTGGCTGGAATGAGGCAGAGGGACTTGATTGAATGGTATGTGAGACAGCAGGACGAGAAAAATGCTTACAGTTCCAAGGAGGAGGCAGAGCGTGAAGTCACCATAATCAGTGCCATCATAGAGTGGTTGATAACAAGAGAAGGACATTTGATAGTACTAGATGATGGTAGGCAAGCAGCAGAAGGCGAGGGTGAAGGCGAAGGTGTAGGTCAAGGCGAAGGTGAAGGTGAAGGTGCAGGTGCAGGGCAACCATCAAAAAAATCTAGAAAAGAAAGAATATTGACTGTGAATCCCAACTATGTCATAGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000347 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016043 GO:0032392 GO:0032508 GO:0032991 GO:0034641 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0051276 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:1901360 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03030 ko04110 ko04111 ko04113 map03030 map04110 map04111 map04113

Pfam Domains

Protein Families

MCM MCM_N MCM_OB

Protein Analysis

832

Amino Acids

92.57

Weight (kDa)

5.71

Isoelectric Point (pI)

34.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM_N	PF14551	20 - 112	9.2e-15	MCM N-terminal domain
MCM_OB	PF17207	119 - 247	7.6e-41	MCM OB domain
MCM	PF00493	332 - 554	2.6e-102	MCM P-loop domain
Mg_chelatase	PF01078	441 - 531	2e-06	Magnesium chelatase, subunit ChlI
MCM_lid	PF17855	569 - 653	3e-23	MCM AAA-lid domain
WHD_MCM6	PF18263	696 - 830	1.6e-19	MCM6 C-terminal winged-helix domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008771)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G44635 AT5G44635
fragaria_vesca	FvH4_3g13360 FvH4_3g13360 FvH4_3g13360 FvH4_3g13360
malus_domestica	MD05G1242400.v1.1 MD10G1224200.v1.1
prunus_persica	Prupe.4G121000_v2.0.a1 Prupe.4G121000_v2.0.a1 Prupe.4G121000_v2.0.a1
pyrus_communis	pycom05g21890 pycom10g18820
rosa_chinensis	RchiOBHm_Chr5g0022071
rosa_laevigata	RLG00000032641
rosa_multiflora	Rmu_sc0006966.1_g000002
rosa_roxburghii	Rroxscaffold_1G00056860
rosa_rugosa	Rorug05G0070900
rosa_samantha	Rh5AG158900 Rh5BG158200 Rh5CG172600 Rh5DG158400
rosa_wichuraiana	Rw5G014180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	1322, 2411
AasI	GACNNNNNNGTC	1 cut(s)	320
AatII	GACGTC	1 cut(s)	623
Acc16I	TGCGCA	1 cut(s)	1195
Acc36I	ACCTGC	3 cut(s)	643, 1322, 2411
AccB1I	GGYRCC	1 cut(s)	1334
AccB7I	CCANNNNNTGG	1 cut(s)	181
AccI	GTMKAC	2 cut(s)	919, 1217
AccII	CGCG	1 cut(s)	1671
AccIII	TCCGGA	1 cut(s)	936
AclI	AACGTT	1 cut(s)	1714
AclWI	GGATC	4 cut(s)	79, 92, 1233, 1407
AcoI	YGGCCR	2 cut(s)	135, 1516
AcsI	RAATTY	5 cut(s)	584, 895, 1316, 1376, 1691
AcuI	CTGAAG	3 cut(s)	64, 1005, 1892
AcyI	GRCGYC	2 cut(s)	477, 620
AfaI	GTAC	3 cut(s)	509, 606, 2340
AfiI	CCNNNNNNNGG	6 cut(s)	95, 181, 205, 1310, 1514, 2181
AgsI	TTSAA	9 cut(s)	190, 247, 334, 457, 505, 1007, 1036, 1991, 2216
AjiI	CACGTC	1 cut(s)	178
AjnI	CCWGG	4 cut(s)	695, 766, 1816, 2124
AluBI	AGCT	5 cut(s)	277, 439, 1042, 1708, 1771
AluI	AGCT	5 cut(s)	277, 439, 1042, 1708, 1771
Alw26I	GTCTC	6 cut(s)	349, 611, 858, 1152, 1176, 2221
AlwI	GGATC	4 cut(s)	79, 92, 1233, 1407
Ama87I	CYCGRG	1 cut(s)	1186
Aor13HI	TCCGGA	1 cut(s)	936
AoxI	GGCC	7 cut(s)	135, 207, 693, 699, 759, 1516, 2127
ApeKI	GCWGC	6 cut(s)	274, 1271, 1496, 1499, 2072, 2360
ApoI	RAATTY	5 cut(s)	584, 895, 1316, 1376, 1691
Asp700I	GAANNNNTTC	1 cut(s)	2109
AspLEI	GCGC	3 cut(s)	807, 1196, 1671
AspS9I	GGNCC	3 cut(s)	417, 699, 2128
AsuC2I	CCSGG	2 cut(s)	90, 2176
AsuHPI	GGTGA	9 cut(s)	52, 421, 716, 1634, 1817, 2275, 2387, 2417, 2423
AvaI	CYCGRG	1 cut(s)	1186
AvaII	GGWCC	1 cut(s)	417
BaeI	ACNNNNGTAYC	2 cut(s)	918, 951
BamHI	GGATCC	1 cut(s)	84
BanI	GGYRCC	1 cut(s)	1334
BanII	GRGCYC	2 cut(s)	240, 644
BarI	GAAGNNNNNNTAC	2 cut(s)	1792, 1824
BbvI	GCAGC	6 cut(s)	286, 1258, 1483, 1486, 2084, 2372
BccI	CCATC	6 cut(s)	62, 908, 2060, 2306, 2342, 2443
BceAI	ACGGC	2 cut(s)	1236, 1503
BcgI	CGANNNNNNTGC	6 cut(s)	247, 281, 1868, 1902, 2228, 2262
BciT130I	CCWGG	4 cut(s)	697, 768, 1818, 2126
BciVI	GTATCC	2 cut(s)	11, 919
BclI	TGATCA	4 cut(s)	1618, 1866, 1962, 2044
BcnI	CCSGG	2 cut(s)	90, 2176
BcoDI	GTCTC	6 cut(s)	349, 611, 858, 1152, 1176, 2221
BfaI	CTAG	5 cut(s)	1764, 1856, 2019, 2342, 2448
BfuAI	ACCTGC	3 cut(s)	643, 1322, 2411
BfuI	GTATCC	2 cut(s)	11, 919
BisI	GCNGC	8 cut(s)	138, 275, 1272, 1497, 1500, 1516, 2073, 2361
BlsI	GCNGC	8 cut(s)	139, 276, 1273, 1498, 1501, 1517, 2074, 2362
BmcAI	AGTACT	1 cut(s)	2340
Bme1390I	CCNGG	6 cut(s)	90, 697, 768, 1818, 2126, 2176
Bme18I	GGWCC	1 cut(s)	417
BmeT110I	CYCGRG	1 cut(s)	1186
BmgBI	CACGTC	1 cut(s)	178
BmgT120I	GGNCC	3 cut(s)	417, 699, 2128
BmiI	GGNNCC	3 cut(s)	86, 643, 1336
BmrFI	CCNGG	6 cut(s)	90, 697, 768, 1818, 2126, 2176
BmrI	ACTGGG	1 cut(s)	1198
BmsI	GCATC	6 cut(s)	162, 806, 943, 1152, 1669, 1750
BmuI	ACTGGG	1 cut(s)	1198
BpmI	CTGGAG	1 cut(s)	185
BpuEI	CTTGAG	4 cut(s)	56, 1171, 1526, 1561
BpuMI	CCSGG	2 cut(s)	90, 2176
Bsa29I	ATCGAT	1 cut(s)	2028
BsaBI	GATNNNNATC	1 cut(s)	2043
BsaHI	GRCGYC	2 cut(s)	477, 620
BsaI	GGTCTC	2 cut(s)	858, 1176
BsaJI	CCNNGG	9 cut(s)	88, 132, 445, 696, 766, 1111, 2080, 2124, 2259
BsaWI	WCCGGW	2 cut(s)	433, 936
BsaXI	ACNNNNNCTCC	2 cut(s)	278, 308
Bsc4I	CCNNNNNNNGG	6 cut(s)	95, 181, 205, 1310, 1514, 2181
Bse118I	RCCGGY	1 cut(s)	701
Bse1I	ACTGG	4 cut(s)	420, 599, 663, 1204
Bse3DI	GCAATG	5 cut(s)	814, 1053, 1428, 1635, 1884
Bse8I	GATNNNNATC	1 cut(s)	2043
BseAI	TCCGGA	1 cut(s)	936
BseBI	CCWGG	4 cut(s)	697, 768, 1818, 2126
BseCI	ATCGAT	1 cut(s)	2028
BseDI	CCNNGG	9 cut(s)	88, 132, 445, 696, 766, 1111, 2080, 2124, 2259
BseGI	GGATG	3 cut(s)	667, 797, 958
BseJI	GATNNNNATC	1 cut(s)	2043
BseLI	CCNNNNNNNGG	6 cut(s)	95, 181, 205, 1310, 1514, 2181
BseMI	GCAATG	5 cut(s)	814, 1053, 1428, 1635, 1884
BseMII	CTCAG	4 cut(s)	241, 504, 1770, 1810
BseNI	ACTGG	4 cut(s)	420, 599, 663, 1204
BseRI	GAGGAG	7 cut(s)	111, 114, 188, 1170, 1255, 1989, 2279
BseX3I	CGGCCG	1 cut(s)	1516
BseXI	GCAGC	6 cut(s)	286, 1258, 1483, 1486, 2084, 2372
BseYI	CCCAGC	1 cut(s)	801
BsgI	GTGCAG	4 cut(s)	1686, 2091, 2439, 2445
Bsh1236I	CGCG	1 cut(s)	1671
Bsh1285I	CGRYCG	2 cut(s)	434, 1519
BshFI	GGCC	7 cut(s)	137, 209, 695, 701, 761, 1518, 2129
BshNI	GGYRCC	1 cut(s)	1334
BshVI	ATCGAT	1 cut(s)	2028
BsiEI	CGRYCG	2 cut(s)	434, 1519
BsiHKCI	CYCGRG	1 cut(s)	1186
BsiSI	CCGG	5 cut(s)	90, 434, 702, 937, 2175
BslFI	GGGAC	3 cut(s)	463, 2191, 2219
BslI	CCNNNNNNNGG	6 cut(s)	95, 181, 205, 1310, 1514, 2181
BsmAI	GTCTC	6 cut(s)	349, 611, 858, 1152, 1176, 2221
BsmBI	CGTCTC	2 cut(s)	349, 611
BsmFI	GGGAC	3 cut(s)	463, 2191, 2219
BsmI	GAATGC	3 cut(s)	326, 615, 1480
BsnI	GGCC	7 cut(s)	137, 209, 695, 701, 761, 1518, 2129
Bso31I	GGTCTC	2 cut(s)	858, 1176
BsoBI	CYCGRG	1 cut(s)	1186
Bsp1286I	GDGCHC	2 cut(s)	240, 644
Bsp13I	TCCGGA	1 cut(s)	936
Bsp19I	CCATGG	1 cut(s)	132
BspANI	GGCC	7 cut(s)	137, 209, 695, 701, 761, 1518, 2129
BspCNI	CTCAG	4 cut(s)	240, 505, 1769, 1809
BspDI	ATCGAT	1 cut(s)	2028
BspEI	TCCGGA	1 cut(s)	936
BspFNI	CGCG	1 cut(s)	1671
BspHI	TCATGA	2 cut(s)	1135, 1414
BspLI	GGNNCC	3 cut(s)	86, 643, 1336
BspMI	ACCTGC	3 cut(s)	643, 1322, 2411
BspPI	GGATC	4 cut(s)	79, 92, 1233, 1407
BspQI	GCTCTTC	1 cut(s)	1563
BspT107I	GGYRCC	1 cut(s)	1334
BspTNI	GGTCTC	2 cut(s)	858, 1176
BsrDI	GCAATG	5 cut(s)	814, 1053, 1428, 1635, 1884
BsrFI	RCCGGY	1 cut(s)	701
BsrI	ACTGG	4 cut(s)	420, 599, 663, 1204
BssAI	RCCGGY	1 cut(s)	701
BssECI	CCNNGG	9 cut(s)	88, 132, 445, 696, 766, 1111, 2080, 2124, 2259
BssNAI	GTATAC	1 cut(s)	1218
BssNI	GRCGYC	2 cut(s)	477, 620
BssT1I	CCWWGG	4 cut(s)	132, 1111, 2080, 2259
Bst1107I	GTATAC	1 cut(s)	1218
Bst2UI	CCWGG	4 cut(s)	697, 768, 1818, 2126
Bst4CI	ACNGT	8 cut(s)	712, 730, 1066, 1291, 1846, 1987, 2255, 2470
Bst6I	CTCTTC	4 cut(s)	984, 1563, 1668, 1795
BstACI	GRCGYC	2 cut(s)	477, 620
BstAPI	GCANNNNNTGC	1 cut(s)	1292
BstC8I	GCNNGC	6 cut(s)	256, 640, 1092, 1347, 2187, 2358
BstDEI	CTNAG	6 cut(s)	227, 513, 1746, 1756, 1796, 2144
BstDSI	CCRYGG	2 cut(s)	132, 445
BstENI	CCTNNNNNAGG	1 cut(s)	203
BstF5I	GGATG	3 cut(s)	667, 797, 958
BstFNI	CGCG	1 cut(s)	1671
BstHHI	GCGC	3 cut(s)	807, 1196, 1671
BstMAI	GTCTC	6 cut(s)	349, 611, 858, 1152, 1176, 2221
BstMCI	CGRYCG	2 cut(s)	434, 1519
BstMWI	GCNNNNNNNGC	6 cut(s)	137, 468, 560, 890, 1292, 1666
BstNI	CCWGG	4 cut(s)	697, 768, 1818, 2126
BstNSI	RCATGY	1 cut(s)	543
BstSCI	CCNGG	6 cut(s)	88, 695, 766, 1816, 2124, 2174
BstUI	CGCG	1 cut(s)	1671
BstV1I	GCAGC	6 cut(s)	286, 1258, 1483, 1486, 2084, 2372
BstX2I	RGATCY	2 cut(s)	84, 1238
BstXI	CCANNNNNNTGG	2 cut(s)	658, 809
BstYI	RGATCY	2 cut(s)	84, 1238
BstZ17I	GTATAC	1 cut(s)	1218
BstZI	CGGCCG	1 cut(s)	1516
Bsu15I	ATCGAT	1 cut(s)	2028
BsuI	GTATCC	2 cut(s)	11, 919
BsuRI	GGCC	7 cut(s)	137, 209, 695, 701, 761, 1518, 2129
BsuTUI	ATCGAT	1 cut(s)	2028
BtgI	CCRYGG	2 cut(s)	132, 445
BtrI	CACGTC	1 cut(s)	178
BtsCI	GGATG	3 cut(s)	667, 797, 958
BtsI	GCAGTG	2 cut(s)	1698, 1944
BveI	ACCTGC	3 cut(s)	643, 1322, 2411
Cac8I	GCNNGC	6 cut(s)	256, 640, 1092, 1347, 2187, 2358
CciI	TCATGA	2 cut(s)	1135, 1414
CfoI	GCGC	3 cut(s)	807, 1196, 1671
Cfr10I	RCCGGY	1 cut(s)	701
Cfr13I	GGNCC	3 cut(s)	417, 699, 2128
ClaI	ATCGAT	1 cut(s)	2028
CseI	GACGC	3 cut(s)	280, 466, 2129
Csp6I	GTAC	3 cut(s)	508, 605, 2339
CviQI	GTAC	3 cut(s)	508, 605, 2339
DdeI	CTNAG	6 cut(s)	227, 513, 1746, 1756, 1796, 2144
DrdI	GACNNNNNNGTC	1 cut(s)	320
DseDI	GACNNNNNNGTC	1 cut(s)	320
EaeI	YGGCCR	2 cut(s)	135, 1516
EagI	CGGCCG	1 cut(s)	1516
Eam1104I	CTCTTC	4 cut(s)	984, 1563, 1668, 1795
EarI	CTCTTC	4 cut(s)	984, 1563, 1668, 1795
EciI	GGCGGA	5 cut(s)	30, 108, 134, 269, 662
EclXI	CGGCCG	1 cut(s)	1516
Eco130I	CCWWGG	4 cut(s)	132, 1111, 2080, 2259
Eco24I	GRGCYC	2 cut(s)	240, 644
Eco31I	GGTCTC	2 cut(s)	858, 1176
Eco47I	GGWCC	1 cut(s)	417
Eco52I	CGGCCG	1 cut(s)	1516
Eco57I	CTGAAG	3 cut(s)	64, 1005, 1892
Eco88I	CYCGRG	1 cut(s)	1186
EcoNI	CCTNNNNNAGG	1 cut(s)	203
EcoO109I	RGGNCCY	1 cut(s)	2128
EcoRI	GAATTC	1 cut(s)	1691
EcoRII	CCWGG	4 cut(s)	695, 766, 1816, 2124
EcoT14I	CCWWGG	4 cut(s)	132, 1111, 2080, 2259
EcoT38I	GRGCYC	2 cut(s)	240, 644
ErhI	CCWWGG	4 cut(s)	132, 1111, 2080, 2259
Esp3I	CGTCTC	2 cut(s)	349, 611
FaqI	GGGAC	3 cut(s)	463, 2191, 2219
FauI	CCCGC	1 cut(s)	631
FbaI	TGATCA	4 cut(s)	1618, 1866, 1962, 2044
FblI	GTMKAC	2 cut(s)	919, 1217
Fnu4HI	GCNGC	8 cut(s)	138, 275, 1272, 1497, 1500, 1516, 2073, 2361
FokI	GGATG	3 cut(s)	674, 784, 965
FriOI	GRGCYC	2 cut(s)	240, 644
Fsp4HI	GCNGC	8 cut(s)	138, 275, 1272, 1497, 1500, 1516, 2073, 2361
FspBI	CTAG	5 cut(s)	1764, 1856, 2019, 2342, 2448
FspI	TGCGCA	1 cut(s)	1195
GlaI	GCGC	3 cut(s)	806, 1195, 1670
GluI	GCNGC	8 cut(s)	138, 275, 1272, 1497, 1500, 1516, 2073, 2361
GsaI	CCCAGC	1 cut(s)	805
GsuI	CTGGAG	1 cut(s)	185
HaeIII	GGCC	7 cut(s)	137, 209, 695, 701, 761, 1518, 2129
HapII	CCGG	5 cut(s)	90, 434, 702, 937, 2175
HgaI	GACGC	3 cut(s)	280, 466, 2129
HhaI	GCGC	3 cut(s)	807, 1196, 1671
Hin1I	GRCGYC	2 cut(s)	477, 620
Hin6I	GCGC	3 cut(s)	805, 1194, 1669
HinP1I	GCGC	3 cut(s)	805, 1194, 1669
HindIII	AAGCTT	1 cut(s)	1040
HinfI	GANTC	7 cut(s)	50, 146, 412, 1411, 1781, 2015, 2473
HpaII	CCGG	5 cut(s)	90, 434, 702, 937, 2175
HphI	GGTGA	9 cut(s)	52, 421, 716, 1634, 1817, 2275, 2387, 2417, 2423
Hpy166II	GTNNAC	8 cut(s)	28, 510, 920, 981, 1123, 1131, 1218, 1246
Hpy8I	GTNNAC	8 cut(s)	28, 510, 920, 981, 1123, 1131, 1218, 1246
Hpy99I	CGWCG	4 cut(s)	65, 221, 302, 965
HpyCH4III	ACNGT	8 cut(s)	712, 730, 1066, 1291, 1846, 1987, 2255, 2470
HpyCH4IV	ACGT	4 cut(s)	177, 620, 1714, 1955
HpyF10VI	GCNNNNNNNGC	6 cut(s)	137, 468, 560, 890, 1292, 1666
HpyF3I	CTNAG	6 cut(s)	227, 513, 1746, 1756, 1796, 2144
HpySE526I	ACGT	4 cut(s)	177, 620, 1714, 1955
Hsp92I	GRCGYC	2 cut(s)	477, 620
HspAI	GCGC	3 cut(s)	805, 1194, 1669
Kpn2I	TCCGGA	1 cut(s)	936
Ksp22I	TGATCA	4 cut(s)	1618, 1866, 1962, 2044
LguI	GCTCTTC	1 cut(s)	1563
LmnI	GCTCC	5 cut(s)	282, 436, 647, 1113, 1819
Lsp1109I	GCAGC	6 cut(s)	286, 1258, 1483, 1486, 2084, 2372
LweI	GCATC	6 cut(s)	162, 806, 943, 1152, 1669, 1750
MaeI	CTAG	5 cut(s)	1764, 1856, 2019, 2342, 2448
MaeII	ACGT	4 cut(s)	177, 620, 1714, 1955
MaeIII	GTNAC	3 cut(s)	268, 409, 2281
MflI	RGATCY	2 cut(s)	84, 1238
MhlI	GDGCHC	2 cut(s)	240, 644
MlyI	GAGTC	5 cut(s)	59, 155, 406, 1790, 2024
MmeI	TCCRAC	3 cut(s)	240, 321, 1159
MroI	TCCGGA	1 cut(s)	936
MroXI	GAANNNNTTC	1 cut(s)	2109
MseI	TTAA	5 cut(s)	846, 1280, 1340, 1751, 2091
MslI	CAYNNNNRTG	1 cut(s)	457
MspI	CCGG	5 cut(s)	90, 434, 702, 937, 2175
MspR9I	CCNGG	6 cut(s)	90, 697, 768, 1818, 2126, 2176
Mva1269I	GAATGC	3 cut(s)	326, 615, 1480
MvaI	CCWGG	4 cut(s)	697, 768, 1818, 2126
MvnI	CGCG	1 cut(s)	1671
MwoI	GCNNNNNNNGC	6 cut(s)	137, 468, 560, 890, 1292, 1666
NciI	CCSGG	2 cut(s)	90, 2176
NcoI	CCATGG	1 cut(s)	132
NlaIV	GGNNCC	3 cut(s)	86, 643, 1336
NmuCI	GTSAC	3 cut(s)	268, 409, 2281
NsbI	TGCGCA	1 cut(s)	1195
NspI	RCATGY	1 cut(s)	543
PagI	TCATGA	2 cut(s)	1135, 1414
PaqCI	CACCTGC	2 cut(s)	1322, 2411
PciSI	GCTCTTC	1 cut(s)	1563
PcsI	WCGNNNNNNNCGW	1 cut(s)	2034
PctI	GAATGC	3 cut(s)	326, 615, 1480
PdmI	GAANNNNTTC	1 cut(s)	2109
PfeI	GAWTC	2 cut(s)	1411, 2473
PflMI	CCANNNNNTGG	1 cut(s)	181
PkrI	GCNGC	8 cut(s)	139, 276, 1273, 1498, 1501, 1517, 2074, 2362
PleI	GAGTC	5 cut(s)	58, 154, 406, 1789, 2023
PpsI	GAGTC	5 cut(s)	58, 154, 406, 1789, 2023
Psp1406I	AACGTT	1 cut(s)	1714
Psp6I	CCWGG	4 cut(s)	695, 766, 1816, 2124
PspFI	CCCAGC	1 cut(s)	801
PspGI	CCWGG	4 cut(s)	695, 766, 1816, 2124
PspN4I	GGNNCC	3 cut(s)	86, 643, 1336
PspPI	GGNCC	3 cut(s)	417, 699, 2128
PsuI	RGATCY	2 cut(s)	84, 1238
RsaI	GTAC	3 cut(s)	509, 606, 2340
RsaNI	GTAC	3 cut(s)	508, 605, 2339
RseI	CAYNNNNRTG	1 cut(s)	457
SapI	GCTCTTC	1 cut(s)	1563
SaqAI	TTAA	5 cut(s)	846, 1280, 1340, 1751, 2091
SatI	GCNGC	8 cut(s)	138, 275, 1272, 1497, 1500, 1516, 2073, 2361
Sau96I	GGNCC	3 cut(s)	417, 699, 2128
ScaI	AGTACT	1 cut(s)	2340
SchI	GAGTC	5 cut(s)	59, 155, 406, 1790, 2024
ScrFI	CCNGG	6 cut(s)	90, 697, 768, 1818, 2126, 2176
SduI	GDGCHC	2 cut(s)	240, 644
SfaNI	GCATC	6 cut(s)	162, 806, 943, 1152, 1669, 1750
SinI	GGWCC	1 cut(s)	417
SmiMI	CAYNNNNRTG	1 cut(s)	457
SmlI	CTYRAG	4 cut(s)	71, 1150, 1541, 1576
SmoI	CTYRAG	4 cut(s)	71, 1150, 1541, 1576
SspI	AATATT	1 cut(s)	2463
SspMI	CTAG	5 cut(s)	1764, 1856, 2019, 2342, 2448
StyD4I	CCNGG	6 cut(s)	88, 695, 766, 1816, 2124, 2174
StyI	CCWWGG	4 cut(s)	132, 1111, 2080, 2259
TaaI	ACNGT	8 cut(s)	712, 730, 1066, 1291, 1846, 1987, 2255, 2470
TaiI	ACGT	4 cut(s)	180, 623, 1717, 1958
TaqI	TCGA	9 cut(s)	63, 126, 165, 430, 492, 790, 1480, 1891, 2028
TatI	WGTACW	2 cut(s)	507, 2338
TauI	GCSGC	2 cut(s)	140, 1518
TfiI	GAWTC	2 cut(s)	1411, 2473
Tru1I	TTAA	5 cut(s)	846, 1280, 1340, 1751, 2091
Tru9I	TTAA	5 cut(s)	846, 1280, 1340, 1751, 2091
TseFI	GTSAC	3 cut(s)	268, 409, 2281
TseI	GCWGC	6 cut(s)	274, 1271, 1496, 1499, 2072, 2360
Tsp45I	GTSAC	3 cut(s)	268, 409, 2281
TspDTI	ATGAA	9 cut(s)	151, 280, 843, 1152, 1389, 1403, 1986, 2118, 2169
TspGWI	ACGGA	2 cut(s)	1231, 1919
Van91I	CCANNNNNTGG	1 cut(s)	181
VpaK11BI	GGWCC	1 cut(s)	417
XagI	CCTNNNNNAGG	1 cut(s)	203
XapI	RAATTY	5 cut(s)	584, 895, 1316, 1376, 1691
XbaI	TCTAGA	1 cut(s)	2447
XceI	RCATGY	1 cut(s)	543
XmiI	GTMKAC	2 cut(s)	919, 1217
XmnI	GAANNNNTTC	1 cut(s)	2109
XspI	CTAG	5 cut(s)	1764, 1856, 2019, 2342, 2448
ZraI	GACGTC	1 cut(s)	621
ZrmI	AGTACT	1 cut(s)	2340

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.