Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0008771

Physical Location & Seq

Genomic Coordinates Forward (+)

16886111 .. 16892503

Gene Structure 6393 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G014180.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2532 bp

ATGGAAGCTTTCGGTGGATTCTTGGTGGACGAGAAGGCGGTTCGGGTCGAGAACATCTTCCTGGACTTCCTCAAAAGCTTCAGACTGGGCGGCGAAGGCGAGGTCTATTACGAGGCCGAGATCGAGGCCATGATCGCCAACGAGTCCACCACCATGTTCATCGACTTCTCTCACGTCATGCTCTTCAATAATCTTCTCCAGAAGGCCATTTCCGACGAGTTCTTGAGGTTCGAGCCGTACTTGAAGAACGCGTGCAAGAGATTTGTGATGGAGCGGCGGTCCAACGCGATTCCGGATGATGTTAACAAGGACATCAATGTTGCCTTCTTCAATCTTCCAGCCTCCAAGAGGCTGAGGGAATTGACGACGGCAGAAATAGGGAAACTGGTGTCGGTGAAAGGAGTGGTGACTCGGACGAGTGAGGTCCGGCCCGAGCTTCTCCACGGGACTTTCAAGTGCTTGGAGTGTGGAGGTGTCATCAAGAATGTTGAACAGCAGTATAAGTACACTGAGCCGACAATTTGTGTCAATGCCACTTGCGCAAATAGAGCAAGGTGGGCATTACTTCGGCAAGAGAGCAAATTTGCGGATTGGCAGAGGGTACGGATGCAGGAGACTTCCAAGGAGATACCTGCGGGCTCCCTTCCCAGATCGCTGGATGTTATAATCCGCCATGAGATTGTTGAGAAGGCCAGGGCTGGTGACACGGTCGTATTTACTGGCACAGTGGTTGTGATACCAGACATATTGGCTTTGGCTGCCCCCGGAGAGAGATCAGAAGTTTCTCGCCAAGCTTCCCAGCGCAGTAATGGTGCGGCTGGGCATGAAGGAGTCAGGGGTCTTCGAGCATTGGGAGTGAGAGATCTCTCCTACCGCCTTGCCTTTATTGCGAATTCAGTTCAGATTTCTGATGGTAGGCAGGATACTGACATACGGAATAGAAAGAAGGATGCTGACGATGATGATCACCAGCAGTTCACTGCAGAAGAGCAAGATGAAGTTCAAAGAATGAGGAATACACCTGATTTCTTCAATAAGATCGTTGATAGCATTGCCCCAACTGTATTTGGTCACCAAGATATCAAGCGAGCAATCCTACTTATGCTCCTGGGTGGTGTCCACAAGTTTACTCATGAAGGTATCAACTTGAGAGGAGATATCAATGTTTGTATAGTAGGGGACCCCAGTTGTGCAAAATCTCAGTTCCTCAAGTATGCTGCTGGAATAGTTCCAAGATCTGTTTACACATCAGGGAAATCCTCCTCTGCTGCTGGTTTAACTGCAACTGTGGCCAAAGAACCAGAAACTGGGGAATTTTGTATTGAGGCTGGCGCATTAATGCTCGCCGACAATGGCATTTGTTGCATTGATGAATTTGACAAGATGGACGTTAGGGATCAGGTTGCAATCCATGAGGCAATGGAACAGCAGACAATAAGCATTACAAAAGCAGGAATACAGGCAACACTGAATGCTCGAACATCAATTCTTGCTGCTGCGAACCCCACAGGGGGCCGTTATGATAAATCTAAACCCCTCAAGTATAACGTGGCTCTTCCTCCTCCTATTTTGTCAAGGTTTGATCTGGTATATGTCATGATCGATGACCCAGATGATCAAACTGATTACCACATAGCCCACCATATTGTAAGAGTACATCAAAAACAGGAAGAGGCGCTTAGTCCTACATTCACTACTGCACAGCTGAAGCGGTATATTGCATATGCCAAAACTCTCAAACCAAAGTTAAATTCAGATGCTAGAAAGCTGTTGGTGGACTCTTATGTTGCTCTCAGAAGAGGTGATACTGCTCCTGGTAGTAGAGTTGCTTATCGCATGACAGTTAGGCAACTAGAGGCATTAATCAGACTCTCAGAGGCCATTGCTCGAAGTTATTTAGAGATTCAGGTTAAGCCACACCATGTCCGTTTAGCAGTGAGATTGCTAAAAACCTCAATAATTAGTGTGGAGTCTAGTGAGATTGATCTATCAGAGTTTGAAGATTCTCGTGACAATGTTGAGGGCAATGACAATGACAATGGAAATAATGGTACTTATCACGGTGATGACAATGGAAATAATGGTACGGGTCCAGGTGATGATCAAACGACCAATGGTAGAGCTTCTGTGAATGGTGAAGGTGGGTCAGGTTCTGCAAACCAACAAGGAAAGAAGCTTATAATAAGTGATGAATATTTTCAGAGGGTTACCCAGGCCCTTATCATGCGTCTGAGACAGCACGAAGAAGATGTTAGACAAAGCGGGACAGGGCTGGCTGGAATGAGGCAGAGGGACTTGATTCAGTGGTATGTGAGTCAGCAGAACGAGAAAAATAATTACGATTTCGTGGAGGAAGCAGCTGCCGAAGTTTCTAAAATCAAAGCTATCATAGAGAGCTTAATAAGAAGAGAAGGGCATCTGATAGTCCTTGATGATGAGAGGCAAGCAGCAGAAGGTGAAGGAGCACCACAGCCACCAGTATCCAGAAATGACAGAATATTGGCTGTGGCTCCTAACTATGTCATTGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000347 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016043 GO:0032392 GO:0032508 GO:0032991 GO:0034641 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0051276 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:1901360 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03030 ko04110 ko04111 ko04113 map03030 map04110 map04111 map04113

Pfam Domains

Protein Families

MCM MCM_N MCM_OB

Protein Analysis

843

Amino Acids

93.71

Weight (kDa)

5.77

Isoelectric Point (pI)

37.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM_N	PF14551	18 - 111	1.6e-16	MCM N-terminal domain
MCM_OB	PF17207	118 - 246	1.2e-40	MCM OB domain
MCM	PF00493	331 - 553	2.9e-102	MCM P-loop domain
Mg_chelatase	PF01078	440 - 530	1.7e-06	Magnesium chelatase, subunit ChlI
MCM_lid	PF17855	568 - 653	5.8e-23	MCM AAA-lid domain
WHD_MCM6	PF18263	724 - 842	5.7e-20	MCM6 C-terminal winged-helix domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0008771)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G44635 AT5G44635
fragaria_vesca	FvH4_3g13360 FvH4_3g13360 FvH4_3g13360 FvH4_3g13360
malus_domestica	MD05G1242400.v1.1 MD10G1224200.v1.1
prunus_persica	Prupe.4G121000_v2.0.a1 Prupe.4G121000_v2.0.a1 Prupe.4G121000_v2.0.a1
pyrus_communis	pycom05g21890 pycom10g18820
rosa_chinensis	RchiOBHm_Chr5g0022071
rosa_laevigata	RLG00000032641
rosa_multiflora	Rmu_sc0006966.1_g000002
rosa_roxburghii	Rroxscaffold_1G00056860
rosa_rugosa	Rorug05G0070900
rosa_samantha	Rh5AG158900 Rh5BG158200 Rh5CG172600 Rh5DG158400
rosa_wichuraiana	Rw5G014180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	665, 2180
Acc16I	TGCGCA	1 cut(s)	541
Acc36I	ACCTGC	1 cut(s)	640
AccB7I	CCANNNNNTGG	1 cut(s)	1307
AccBSI	CCGCTC	1 cut(s)	274
AccII	CGCG	2 cut(s)	251, 287
AccIII	TCCGGA	1 cut(s)	292
AclWI	GGATC	1 cut(s)	1404
AcoI	YGGCCR	1 cut(s)	1290
AcsI	RAATTY	5 cut(s)	581, 892, 1313, 1373, 1750
AcuI	CTGAAG	2 cut(s)	64, 1727
AfaI	GTAC	6 cut(s)	239, 506, 603, 1656, 2053, 2086
AfiI	CCNNNNNNNGG	4 cut(s)	348, 1307, 1510, 1511
AflIII	ACRYGT	1 cut(s)	249
AgsI	TTSAA	8 cut(s)	187, 244, 331, 454, 491, 1004, 1033, 2000
AjiI	CACGTC	1 cut(s)	175
AjnI	CCWGG	6 cut(s)	60, 692, 1107, 1813, 2092, 2211
AjuI	GAANNNNNNNTTGG	2 cut(s)	1735, 1767
Alw21I	GWGCWC	1 cut(s)	2467
Alw26I	GTCTC	2 cut(s)	608, 2227
AlwI	GGATC	1 cut(s)	1404
AlwNI	CAGNNNCTG	2 cut(s)	1307, 2153
Ama87I	CYCGRG	1 cut(s)	431
Aor13HI	TCCGGA	1 cut(s)	292
AoxI	GGCC	9 cut(s)	114, 126, 204, 428, 690, 1290, 1513, 1878, 2214
ApeKI	GCWGC	8 cut(s)	758, 1217, 1268, 1493, 1496, 2357, 2360, 2447
ApoI	RAATTY	5 cut(s)	581, 892, 1313, 1373, 1750
ArsI	GACNNNNNNTTYG	2 cut(s)	87, 119
AseI	ATTAAT	2 cut(s)	1337, 1862
Asp700I	GAANNNNTTC	2 cut(s)	56, 2196
AspLEI	GCGC	4 cut(s)	542, 804, 1334, 1678
AspS9I	GGNCC	7 cut(s)	279, 424, 429, 1180, 1513, 2090, 2215
AsuC2I	CCSGG	1 cut(s)	765
AvaI	CYCGRG	1 cut(s)	431
AvaII	GGWCC	4 cut(s)	279, 424, 1180, 2090
BalI	TGGCCA	1 cut(s)	1292
BanII	GRGCYC	1 cut(s)	641
BarI	GAAGNNNNNNTAC	2 cut(s)	1789, 1821
BauI	CACGAG	1 cut(s)	2007
BbsI	GAAGAC	1 cut(s)	833
Bbv12I	GWGCWC	1 cut(s)	2467
BbvCI	CCTCAGC	1 cut(s)	353
BbvI	GCAGC	8 cut(s)	745, 1204, 1255, 1480, 1483, 2347, 2369, 2459
BccI	CCATC	3 cut(s)	262, 905, 1378
BceAI	ACGGC	3 cut(s)	220, 384, 1500
BciT130I	CCWGG	6 cut(s)	62, 694, 1109, 1815, 2094, 2213
BciVI	GTATCC	2 cut(s)	916, 2491
BclI	TGATCA	3 cut(s)	964, 1615, 2101
BcnI	CCSGG	1 cut(s)	765
BcoDI	GTCTC	2 cut(s)	608, 2227
BfaI	CTAG	3 cut(s)	1761, 1853, 1974
BfmI	CTRYAG	1 cut(s)	981
BfoI	RGCGCY	1 cut(s)	1679
BfuAI	ACCTGC	1 cut(s)	640
BfuI	GTATCC	2 cut(s)	916, 2491
BglII	AGATCT	2 cut(s)	862, 1235
Bme1390I	CCNGG	7 cut(s)	62, 694, 765, 1109, 1815, 2094, 2213
Bme18I	GGWCC	4 cut(s)	279, 424, 1180, 2090
BmeT110I	CYCGRG	1 cut(s)	431
BmgBI	CACGTC	1 cut(s)	175
BmgT120I	GGNCC	7 cut(s)	279, 424, 429, 1180, 1513, 2090, 2215
BmiI	GGNNCC	6 cut(s)	640, 1181, 1182, 1514, 2091, 2511
BmrFI	CCNGG	7 cut(s)	62, 694, 765, 1109, 1815, 2094, 2213
BmrI	ACTGGG	3 cut(s)	95, 1179, 1317
BmsI	GCATC	4 cut(s)	597, 940, 1747, 2425
BmuI	ACTGGG	3 cut(s)	95, 1179, 1317
BpiI	GAAGAC	1 cut(s)	833
BpmI	CTGGAG	1 cut(s)	182
Bpu10I	CCTNAGC	1 cut(s)	353
BpuEI	CTTGAG	4 cut(s)	244, 1168, 1193, 1523
BpuMI	CCSGG	1 cut(s)	765
Bsa29I	ATCGAT	1 cut(s)	1602
BsaBI	GATNNNNATC	1 cut(s)	963
BsaJI	CCNNGG	6 cut(s)	442, 621, 693, 763, 1108, 2211
BsaWI	WCCGGW	1 cut(s)	292
BsaXI	ACNNNNNCTCC	4 cut(s)	263, 293, 1089, 1119
Bsc4I	CCNNNNNNNGG	4 cut(s)	348, 1307, 1510, 1511
Bse1I	ACTGG	6 cut(s)	90, 390, 724, 1185, 1312, 2477
Bse3DI	GCAATG	4 cut(s)	1050, 1425, 1881, 2032
Bse8I	GATNNNNATC	1 cut(s)	963
BseAI	TCCGGA	1 cut(s)	292
BseBI	CCWGG	6 cut(s)	62, 694, 1109, 1815, 2094, 2213
BseCI	ATCGAT	1 cut(s)	1602
BseDI	CCNNGG	6 cut(s)	442, 621, 693, 763, 1108, 2211
BseGI	GGATG	4 cut(s)	301, 612, 664, 955
BseJI	GATNNNNATC	1 cut(s)	963
BseLI	CCNNNNNNNGG	4 cut(s)	348, 1307, 1510, 1511
BseMI	GCAATG	4 cut(s)	1050, 1425, 1881, 2032
BseMII	CTCAG	6 cut(s)	344, 501, 1214, 1807, 1887, 2222
BseNI	ACTGG	6 cut(s)	90, 390, 724, 1185, 1312, 2477
BseRI	GAGGAG	3 cut(s)	1167, 1252, 1551
BseXI	GCAGC	8 cut(s)	745, 1204, 1255, 1480, 1483, 2347, 2369, 2459
BseYI	CCCAGC	2 cut(s)	798, 818
BsgI	GTGCAG	1 cut(s)	1683
Bsh1236I	CGCG	2 cut(s)	251, 287
Bsh1285I	CGRYCG	1 cut(s)	711
BshFI	GGCC	9 cut(s)	116, 128, 206, 430, 692, 1292, 1515, 1880, 2216
BshVI	ATCGAT	1 cut(s)	1602
BsiEI	CGRYCG	1 cut(s)	711
BsiHKAI	GWGCWC	1 cut(s)	2467
BsiHKCI	CYCGRG	1 cut(s)	431
BsiSI	CCGG	3 cut(s)	293, 427, 765
BslFI	GGGAC	4 cut(s)	460, 1193, 2278, 2306
BslI	CCNNNNNNNGG	4 cut(s)	348, 1307, 1510, 1511
BsmAI	GTCTC	2 cut(s)	608, 2227
BsmFI	GGGAC	4 cut(s)	460, 1193, 2278, 2306
BsmI	GAATGC	1 cut(s)	1477
BsnI	GGCC	9 cut(s)	116, 128, 206, 430, 692, 1292, 1515, 1880, 2216
BsoBI	CYCGRG	1 cut(s)	431
Bsp1286I	GDGCHC	2 cut(s)	641, 2467
Bsp13I	TCCGGA	1 cut(s)	292
BspANI	GGCC	9 cut(s)	116, 128, 206, 430, 692, 1292, 1515, 1880, 2216
BspCNI	CTCAG	6 cut(s)	345, 502, 1213, 1806, 1886, 2223
BspDI	ATCGAT	1 cut(s)	1602
BspEI	TCCGGA	1 cut(s)	292
BspFNI	CGCG	2 cut(s)	251, 287
BspHI	TCATGA	2 cut(s)	1132, 1596
BspLI	GGNNCC	6 cut(s)	640, 1181, 1182, 1514, 2091, 2511
BspMAI	CTGCAG	1 cut(s)	985
BspMI	ACCTGC	1 cut(s)	640
BspPI	GGATC	1 cut(s)	1404
BspQI	GCTCTTC	3 cut(s)	188, 981, 1560
BsrBI	CCGCTC	1 cut(s)	274
BsrDI	GCAATG	4 cut(s)	1050, 1425, 1881, 2032
BsrI	ACTGG	6 cut(s)	90, 390, 724, 1185, 1312, 2477
BssECI	CCNNGG	6 cut(s)	442, 621, 693, 763, 1108, 2211
BssSI	CACGAG	1 cut(s)	2007
BssT1I	CCWWGG	1 cut(s)	621
Bst2BI	CACGAG	1 cut(s)	2007
Bst2UI	CCWGG	6 cut(s)	62, 694, 1109, 1815, 2094, 2213
Bst4CI	ACNGT	6 cut(s)	709, 727, 1063, 1288, 1843, 2063
Bst6I	CTCTTC	6 cut(s)	188, 981, 1560, 1665, 1792, 2401
BstAPI	GCANNNNNTGC	1 cut(s)	1362
BstC8I	GCNNGC	7 cut(s)	253, 637, 1089, 1330, 1344, 2274, 2445
BstDEI	CTNAG	7 cut(s)	353, 510, 1200, 1679, 1793, 1873, 2231
BstDSI	CCRYGG	1 cut(s)	442
BstEII	GGTNACC	2 cut(s)	1070, 2206
BstENI	CCTNNNNNAGG	1 cut(s)	346
BstF5I	GGATG	4 cut(s)	301, 612, 664, 955
BstFNI	CGCG	2 cut(s)	251, 287
BstH2I	RGCGCY	1 cut(s)	1679
BstHHI	GCGC	4 cut(s)	542, 804, 1334, 1678
BstMAI	GTCTC	2 cut(s)	608, 2227
BstMCI	CGRYCG	1 cut(s)	711
BstMWI	GCNNNNNNNGC	9 cut(s)	96, 134, 539, 548, 557, 758, 887, 1289, 1362
BstNI	CCWGG	6 cut(s)	62, 694, 1109, 1815, 2094, 2213
BstPI	GGTNACC	2 cut(s)	1070, 2206
BstSCI	CCNGG	7 cut(s)	60, 692, 763, 1107, 1813, 2092, 2211
BstSFI	CTRYAG	1 cut(s)	981
BstUI	CGCG	2 cut(s)	251, 287
BstV1I	GCAGC	8 cut(s)	745, 1204, 1255, 1480, 1483, 2347, 2369, 2459
BstV2I	GAAGAC	1 cut(s)	833
BstX2I	RGATCY	2 cut(s)	862, 1235
BstXI	CCANNNNNNTGG	1 cut(s)	655
BstYI	RGATCY	2 cut(s)	862, 1235
Bsu15I	ATCGAT	1 cut(s)	1602
BsuI	GTATCC	2 cut(s)	916, 2491
BsuRI	GGCC	9 cut(s)	116, 128, 206, 430, 692, 1292, 1515, 1880, 2216
BsuTUI	ATCGAT	1 cut(s)	1602
BtgI	CCRYGG	1 cut(s)	442
BtrI	CACGTC	1 cut(s)	175
BtsCI	GGATG	4 cut(s)	301, 612, 664, 955
BtsI	GCAGTG	2 cut(s)	978, 1941
BtsIMutI	CAGTG	6 cut(s)	507, 732, 978, 1466, 1941, 2309
BveI	ACCTGC	1 cut(s)	640
Cac8I	GCNNGC	7 cut(s)	253, 637, 1089, 1330, 1344, 2274, 2445
CaiI	CAGNNNCTG	2 cut(s)	1307, 2153
CciI	TCATGA	2 cut(s)	1132, 1596
CfoI	GCGC	4 cut(s)	542, 804, 1334, 1678
Cfr13I	GGNCC	7 cut(s)	279, 424, 429, 1180, 1513, 2090, 2215
ClaI	ATCGAT	1 cut(s)	1602
CseI	GACGC	1 cut(s)	2216
Csp6I	GTAC	6 cut(s)	238, 505, 602, 1655, 2052, 2085
CviQI	GTAC	6 cut(s)	238, 505, 602, 1655, 2052, 2085
DdeI	CTNAG	7 cut(s)	353, 510, 1200, 1679, 1793, 1873, 2231
EaeI	YGGCCR	1 cut(s)	1290
Eam1104I	CTCTTC	6 cut(s)	188, 981, 1560, 1665, 1792, 2401
EarI	CTCTTC	6 cut(s)	188, 981, 1560, 1665, 1792, 2401
EciI	GGCGGA	1 cut(s)	659
Eco130I	CCWWGG	1 cut(s)	621
Eco24I	GRGCYC	1 cut(s)	641
Eco32I	GATATC	2 cut(s)	1081, 1159
Eco47I	GGWCC	4 cut(s)	279, 424, 1180, 2090
Eco57I	CTGAAG	2 cut(s)	64, 1727
Eco88I	CYCGRG	1 cut(s)	431
Eco91I	GGTNACC	2 cut(s)	1070, 2206
EcoNI	CCTNNNNNAGG	1 cut(s)	346
EcoO109I	RGGNCCY	2 cut(s)	1180, 2215
EcoO65I	GGTNACC	2 cut(s)	1070, 2206
EcoRI	GAATTC	1 cut(s)	892
EcoRII	CCWGG	6 cut(s)	60, 692, 1107, 1813, 2092, 2211
EcoRV	GATATC	2 cut(s)	1081, 1159
EcoT14I	CCWWGG	1 cut(s)	621
EcoT38I	GRGCYC	1 cut(s)	641
ErhI	CCWWGG	1 cut(s)	621
FalI	AAGNNNNNCTT	2 cut(s)	1662, 1694
FaqI	GGGAC	4 cut(s)	460, 1193, 2278, 2306
FauI	CCCGC	2 cut(s)	628, 2255
FauNDI	CATATG	1 cut(s)	1723
FbaI	TGATCA	3 cut(s)	964, 1615, 2101
FokI	GGATG	4 cut(s)	308, 619, 671, 962
FriOI	GRGCYC	1 cut(s)	641
FspBI	CTAG	3 cut(s)	1761, 1853, 1974
FspI	TGCGCA	1 cut(s)	541
GlaI	GCGC	4 cut(s)	541, 803, 1333, 1677
GsaI	CCCAGC	2 cut(s)	802, 822
GsuI	CTGGAG	1 cut(s)	182
HaeII	RGCGCY	1 cut(s)	1679
HaeIII	GGCC	9 cut(s)	116, 128, 206, 430, 692, 1292, 1515, 1880, 2216
HapII	CCGG	3 cut(s)	293, 427, 765
HgaI	GACGC	1 cut(s)	2216
HhaI	GCGC	4 cut(s)	542, 804, 1334, 1678
Hin6I	GCGC	4 cut(s)	540, 802, 1332, 1676
HinP1I	GCGC	4 cut(s)	540, 802, 1332, 1676
HincII	GTYRAC	1 cut(s)	304
HindII	GTYRAC	1 cut(s)	304
HindIII	AAGCTT	4 cut(s)	6, 76, 792, 2174
HpaI	GTTAAC	1 cut(s)	304
HpaII	CCGG	3 cut(s)	293, 427, 765
Hpy166II	GTNNAC	9 cut(s)	28, 147, 304, 507, 978, 1120, 1128, 1243, 1777
Hpy188III	TCNNGA	9 cut(s)	49, 199, 223, 293, 481, 1133, 1597, 2009, 2484
Hpy8I	GTNNAC	9 cut(s)	28, 147, 304, 507, 978, 1120, 1128, 1243, 1777
Hpy99I	CGWCG	2 cut(s)	218, 370
HpyCH4III	ACNGT	6 cut(s)	709, 727, 1063, 1288, 1843, 2063
HpyCH4IV	ACGT	3 cut(s)	174, 1389, 1548
HpyF10VI	GCNNNNNNNGC	9 cut(s)	96, 134, 539, 548, 557, 758, 887, 1289, 1362
HpyF3I	CTNAG	7 cut(s)	353, 510, 1200, 1679, 1793, 1873, 2231
HpySE526I	ACGT	3 cut(s)	174, 1389, 1548
HspAI	GCGC	4 cut(s)	540, 802, 1332, 1676
KflI	GGGWCCC	1 cut(s)	1180
Kpn2I	TCCGGA	1 cut(s)	292
Ksp22I	TGATCA	3 cut(s)	964, 1615, 2101
KspAI	GTTAAC	1 cut(s)	304
LguI	GCTCTTC	3 cut(s)	188, 981, 1560
LmnI	GCTCC	6 cut(s)	271, 644, 1110, 1816, 2462, 2515
Lsp1109I	GCAGC	8 cut(s)	745, 1204, 1255, 1480, 1483, 2347, 2369, 2459
LweI	GCATC	4 cut(s)	597, 940, 1747, 2425
MaeI	CTAG	3 cut(s)	1761, 1853, 1974
MaeII	ACGT	3 cut(s)	174, 1389, 1548
MaeIII	GTNAC	5 cut(s)	406, 701, 1070, 2009, 2206
MbiI	CCGCTC	1 cut(s)	274
MflI	RGATCY	2 cut(s)	862, 1235
MhlI	GDGCHC	2 cut(s)	641, 2467
MlsI	TGGCCA	1 cut(s)	1292
MluI	ACGCGT	1 cut(s)	249
MluNI	TGGCCA	1 cut(s)	1292
MlyI	GAGTC	7 cut(s)	152, 403, 840, 1772, 1863, 1979, 2323
MmeI	TCCRAC	2 cut(s)	237, 306
Mox20I	TGGCCA	1 cut(s)	1292
MroI	TCCGGA	1 cut(s)	292
MroXI	GAANNNNTTC	2 cut(s)	56, 2196
MscI	TGGCCA	1 cut(s)	1292
MseI	TTAA	7 cut(s)	303, 1277, 1337, 1748, 1862, 1911, 2399
MslI	CAYNNNNRTG	2 cut(s)	152, 2064
Msp20I	TGGCCA	1 cut(s)	1292
MspA1I	CMGCKG	2 cut(s)	1705, 2360
MspI	CCGG	3 cut(s)	293, 427, 765
MspR9I	CCNGG	7 cut(s)	62, 694, 765, 1109, 1815, 2094, 2213
Mva1269I	GAATGC	1 cut(s)	1477
MvaI	CCWGG	6 cut(s)	62, 694, 1109, 1815, 2094, 2213
MvnI	CGCG	2 cut(s)	251, 287
MwoI	GCNNNNNNNGC	9 cut(s)	96, 134, 539, 548, 557, 758, 887, 1289, 1362
NciI	CCSGG	1 cut(s)	765
NdeI	CATATG	1 cut(s)	1723
NlaIV	GGNNCC	6 cut(s)	640, 1181, 1182, 1514, 2091, 2511
NmeAIII	GCCGAG	1 cut(s)	142
NmuCI	GTSAC	4 cut(s)	406, 701, 1070, 2009
NsbI	TGCGCA	1 cut(s)	541
PagI	TCATGA	2 cut(s)	1132, 1596
PciSI	GCTCTTC	3 cut(s)	188, 981, 1560
PctI	GAATGC	1 cut(s)	1477
PdmI	GAANNNNTTC	2 cut(s)	56, 2196
PfeI	GAWTC	5 cut(s)	18, 289, 1903, 2003, 2299
PflFI	GACNNNGTC	1 cut(s)	707
PflMI	CCANNNNNTGG	1 cut(s)	1307
PfoI	TCCNGGA	1 cut(s)	60
PleI	GAGTC	7 cut(s)	151, 403, 839, 1772, 1863, 1978, 2322
PpsI	GAGTC	7 cut(s)	151, 403, 839, 1772, 1863, 1978, 2322
PpuMI	RGGWCCY	1 cut(s)	1180
PshBI	ATTAAT	2 cut(s)	1337, 1862
PsiI	TTATAA	2 cut(s)	665, 2180
Psp5II	RGGWCCY	1 cut(s)	1180
Psp6I	CCWGG	6 cut(s)	60, 692, 1107, 1813, 2092, 2211
PspEI	GGTNACC	2 cut(s)	1070, 2206
PspFI	CCCAGC	2 cut(s)	798, 818
PspGI	CCWGG	6 cut(s)	60, 692, 1107, 1813, 2092, 2211
PspN4I	GGNNCC	6 cut(s)	640, 1181, 1182, 1514, 2091, 2511
PspPI	GGNCC	7 cut(s)	279, 424, 429, 1180, 1513, 2090, 2215
PspPPI	RGGWCCY	1 cut(s)	1180
PstI	CTGCAG	1 cut(s)	985
PstNI	CAGNNNCTG	2 cut(s)	1307, 2153
PsuI	RGATCY	2 cut(s)	862, 1235
PsyI	GACNNNGTC	1 cut(s)	707
PvuII	CAGCTG	2 cut(s)	1705, 2360
RsaI	GTAC	6 cut(s)	239, 506, 603, 1656, 2053, 2086
RsaNI	GTAC	6 cut(s)	238, 505, 602, 1655, 2052, 2085
RseI	CAYNNNNRTG	2 cut(s)	152, 2064
SapI	GCTCTTC	3 cut(s)	188, 981, 1560
SaqAI	TTAA	7 cut(s)	303, 1277, 1337, 1748, 1862, 1911, 2399
Sau96I	GGNCC	7 cut(s)	279, 424, 429, 1180, 1513, 2090, 2215
SchI	GAGTC	7 cut(s)	152, 403, 840, 1772, 1863, 1979, 2323
ScrFI	CCNGG	7 cut(s)	62, 694, 765, 1109, 1815, 2094, 2213
SduI	GDGCHC	2 cut(s)	641, 2467
SfaNI	GCATC	4 cut(s)	597, 940, 1747, 2425
SfcI	CTRYAG	1 cut(s)	981
SinI	GGWCC	4 cut(s)	279, 424, 1180, 2090
SmiMI	CAYNNNNRTG	2 cut(s)	152, 2064
SmlI	CTYRAG	4 cut(s)	223, 1147, 1208, 1538
SmoI	CTYRAG	4 cut(s)	223, 1147, 1208, 1538
SspI	AATATT	2 cut(s)	2195, 2499
SspMI	CTAG	3 cut(s)	1761, 1853, 1974
StyD4I	CCNGG	7 cut(s)	60, 692, 763, 1107, 1813, 2092, 2211
StyI	CCWWGG	1 cut(s)	621
TaaI	ACNGT	6 cut(s)	709, 727, 1063, 1288, 1843, 2063
TaiI	ACGT	3 cut(s)	177, 1392, 1551
TaqI	TCGA	8 cut(s)	48, 123, 162, 231, 844, 1477, 1602, 1888
TatI	WGTACW	2 cut(s)	504, 1654
TauI	GCSGC	3 cut(s)	93, 277, 818
TfiI	GAWTC	5 cut(s)	18, 289, 1903, 2003, 2299
Tru1I	TTAA	7 cut(s)	303, 1277, 1337, 1748, 1862, 1911, 2399
Tru9I	TTAA	7 cut(s)	303, 1277, 1337, 1748, 1862, 1911, 2399
TscAI	CASTG	6 cut(s)	514, 732, 985, 1473, 1941, 2309
TseFI	GTSAC	4 cut(s)	406, 701, 1070, 2009
TseI	GCWGC	8 cut(s)	758, 1217, 1268, 1493, 1496, 2357, 2360, 2447
Tsp45I	GTSAC	4 cut(s)	406, 701, 1070, 2009
TspDTI	ATGAA	6 cut(s)	148, 840, 1011, 1149, 1386, 2205
TspGWI	ACGGA	3 cut(s)	619, 949, 1916
TspRI	CASTG	6 cut(s)	514, 732, 985, 1473, 1941, 2309
Tth111I	GACNNNGTC	1 cut(s)	707
Van91I	CCANNNNNTGG	1 cut(s)	1307
VpaK11BI	GGWCC	4 cut(s)	279, 424, 1180, 2090
VspI	ATTAAT	2 cut(s)	1337, 1862
XagI	CCTNNNNNAGG	1 cut(s)	346
XapI	RAATTY	5 cut(s)	581, 892, 1313, 1373, 1750
XcmI	CCANNNNNNNNNTGG	1 cut(s)	806
XmnI	GAANNNNTTC	2 cut(s)	56, 2196
XspI	CTAG	3 cut(s)	1761, 1853, 1974

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.