Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5C

Orthogroup ID

OG0008771

Physical Location & Seq

Genomic Coordinates Forward (+)

16316853 .. 16323605

Gene Structure 6753 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5CG172600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2295 bp

ATGGAAGCTTTCGGTGGATTCTTGGTGGACGAGAAGGCGGTTCGGGTCGAGAACATCTTCCTGGACTTCCTCAAAAGCTTCAGACTGGGCGGCGAAGGCGAGGTCTATTACGAGGCCGAGATCGAGGCCATGATCGCCAACGAGTCCACCACCATGTTCATCGACTTCTCTCACGTCATGCTCTTCAATAATCTTCTCCAGAAGGCCATTTCCGACGAGTTCTTGAGGTTTGAGCCATACTTGAAGAACGCGTGCAAGAGATTTGTGATGGAGCGGCGGTCCAACGCGATTCCGGATGATGTTAACAAGGACATCAATGTTGCCTTCTTCAATCTTCCAGCCTCCAAGAGGCTGAGGGAATTGACGACGGCAGAAATAGGGAAACTGGTGTCGGTGAAAGGAGTGGTGACTCGGACGAGTGAGGTCCGGCCCGAGCTTCTCCACGGGACTTTTAAGTGCTTGGAGTGTGGAGGTGTCATTAAGAATGTTGAACAGCAGTATAAGTACACTGAGCCGACAATTTGTGTCAATGCCACTTGCGCAAATAGAGCAAGGTGGGCATTACTTCGGCAAGAGAGCAAATTTGCGGATTGGCAGAGGGTACGGATGCAGGAGACTTCCAAGGAGATACCTGCGGGCTCCCTTCCCAGATCGCTGGATGTTATAATCCGCCATGAGATTGTTGAGAAGGCCAGGGCTGGTGACACGGTCATATTTACCGGCACAGTGGTTGTGATACCAGACATATTGGCTTTGGCTGCCGCCGGAGAGAGATCAGAAGTTTCTCGCCAAGCTTCCCAGCGCAGTAATGGTGCGGCTGGGCATGAAGGAGTCAGGGGTCTTCGAGCATTGGGAGTGAGAGACCTCTCCTACCGCCTTGCCTTTATTGCGAATTCAGTTCAGATTTCTGATGGTAGGCAGGATACTGACATACGGAATAGAAAGAAGGATGCTGACGATGATGATCACCAGCAGTTCACTGCAGAAGAGCAAGATGAAGTTCAAAGAATGAGGAATACACCTGATTTCTTCAATAAGATCGTTGATAGCATTGCCCCAACTGTATTTGGCCACCAAGATATCAAGCGAGCAATCCTACTTATGCTCCTGGGTGGTGTCCACAAGTTTACTCATGAAGGTATCAACTTGAGAGGAGATATCAATGTTTGTATAGTAGGGGACCCCAGTTGTGCAAAATCGCAGTTCCTCAAGTATGCTGCTGGAATAGTTCCAAGATCTGTTTACACATCAGGGAAATCCTCCTCTGCTGCTGGTTTAACTGCAACTGTGGCCAAAGAACCAGAAACTGGGGAATTTTGTATTGAGGCTGGAGCATTAATGCTCGCCGACAATGGCATTTGTTGCATTGATGAATTTGACAAGATGGACGTTAGGGATCAGGTTGCAATTCATGAGGCAATGGAACAGCAAACAATAAGCATTACAAAAGCAGGAATACAGGCAACACTGAATGCTCGAACATCAATTCTTGCTGCTGCGAACCCCACAGGGGGCCGTTATGATAAATCTAAACCCCTCAAGTATAACGTGGCTCTTCCTCCTCCTATTTTGTCAAGGTTTGATCTGGTATATGTCATGATCGATGACCCAGATGATCAAACTGATTACCACATAGCCCACCATATTGTAAGAGTACATCAAAAACGGGAAGAGGCGCTTAGTCCTACATTCACTACTGCACAGCTGAAGCGGTATATTGCATATGCCAAAACTCTCAAACCAAAGTTAAATTCAGATGCTAGAAAGCTGTTGGTGGACTCTTATGTTGCTCTCAGAAGAGGTGATACTGCTCCTGGTAGTAGAGTTGCTTATCGCATGACAGTTAGGCAACTAGAGGCATTAATCAGACTCTCAGAGGCCATTGCTCGAAGTTATTTAGAGATTCAGGTTAAGCCACACCATGTCCGTTTAGCAGTGAGATTGCTAAAAACCTCAATAATTAGTGTGGAGTCTAGTGAGATTGATCTATCAGAGTTTGAAGATTCTCGTGACAATGTTGAGGGCAATGACAATGACAATGGAAATAATGGTACTGATAACGGTGATGACGATGGAAATAATGGTACGGGTCCAGGTGATGATCAAACAACCAATGGCAGAGCTTCTGTGAATGGTGGTGGGTCAGGTTCTGCAAACCAACAAGGAAAGAAGCTTATAATAAGTGATGAATATTTTCAGAGGGTTACCCAGGCCCTTATCATGCGTCTGAGACAGCACGAAGAAGATGTTAGACAAAGCGGTAAGCCATATGCTCTGTTTATTATTTTCATATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000347 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016043 GO:0032392 GO:0032508 GO:0032991 GO:0034641 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0051276 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:1901360 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03030 ko04110 ko04111 ko04113 map03030 map04110 map04111 map04113

Pfam Domains

Protein Families

MCM MCM_N MCM_OB

Protein Analysis

764

Amino Acids

84.86

Weight (kDa)

6.05

Isoelectric Point (pI)

35.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM_N	PF14551	18 - 111	1.4e-16	MCM N-terminal domain
MCM_OB	PF17207	118 - 246	8.7e-41	MCM OB domain
MCM	PF00493	331 - 553	2.4e-102	MCM P-loop domain
Mg_chelatase	PF01078	440 - 530	1.3e-06	Magnesium chelatase, subunit ChlI
MCM_lid	PF17855	568 - 653	5.1e-23	MCM AAA-lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008771)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G44635 AT5G44635
fragaria_vesca	FvH4_3g13360 FvH4_3g13360 FvH4_3g13360 FvH4_3g13360
malus_domestica	MD05G1242400.v1.1 MD10G1224200.v1.1
prunus_persica	Prupe.4G121000_v2.0.a1 Prupe.4G121000_v2.0.a1 Prupe.4G121000_v2.0.a1
pyrus_communis	pycom05g21890 pycom10g18820
rosa_chinensis	RchiOBHm_Chr5g0022071
rosa_laevigata	RLG00000032641
rosa_multiflora	Rmu_sc0006966.1_g000002
rosa_roxburghii	Rroxscaffold_1G00056860
rosa_rugosa	Rorug05G0070900
rosa_samantha	Rh5AG158900 Rh5BG158200 Rh5CG172600 Rh5DG158400
rosa_wichuraiana	Rw5G014180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	665, 2177
Acc16I	TGCGCA	1 cut(s)	541
Acc36I	ACCTGC	1 cut(s)	640
AccB7I	CCANNNNNTGG	1 cut(s)	1307
AccBSI	CCGCTC	1 cut(s)	274
AccII	CGCG	2 cut(s)	251, 287
AccIII	TCCGGA	1 cut(s)	292
AclWI	GGATC	1 cut(s)	1404
AcoI	YGGCCR	2 cut(s)	1069, 1290
AcsI	RAATTY	5 cut(s)	581, 892, 1313, 1373, 1750
AcuI	CTGAAG	2 cut(s)	64, 1727
AfaI	GTAC	5 cut(s)	506, 603, 1656, 2053, 2086
AfiI	CCNNNNNNNGG	4 cut(s)	348, 1307, 1510, 1511
AflIII	ACRYGT	1 cut(s)	249
AgsI	TTSAA	7 cut(s)	187, 244, 331, 491, 1004, 1033, 2000
AjiI	CACGTC	1 cut(s)	175
AjnI	CCWGG	6 cut(s)	60, 692, 1107, 1813, 2092, 2208
AjuI	GAANNNNNNNTTGG	2 cut(s)	1735, 1767
AluBI	AGCT	8 cut(s)	8, 78, 436, 794, 1705, 1768, 2123, 2173
AluI	AGCT	8 cut(s)	8, 78, 436, 794, 1705, 1768, 2123, 2173
Alw26I	GTCTC	3 cut(s)	608, 855, 2224
AlwI	GGATC	1 cut(s)	1404
AlwNI	CAGNNNCTG	2 cut(s)	1307, 2150
Ama87I	CYCGRG	1 cut(s)	431
Aor13HI	TCCGGA	1 cut(s)	292
ApeKI	GCWGC	5 cut(s)	758, 1217, 1268, 1493, 1496
ApoI	RAATTY	5 cut(s)	581, 892, 1313, 1373, 1750
ArsI	GACNNNNNNTTYG	2 cut(s)	87, 119
AseI	ATTAAT	2 cut(s)	1337, 1862
Asp700I	GAANNNNTTC	2 cut(s)	56, 2193
AspLEI	GCGC	3 cut(s)	542, 804, 1678
AspS9I	GGNCC	7 cut(s)	279, 424, 429, 1180, 1513, 2090, 2212
AsuHPI	GGTGA	7 cut(s)	406, 418, 713, 959, 1814, 2075, 2108
AvaI	CYCGRG	1 cut(s)	431
AvaII	GGWCC	4 cut(s)	279, 424, 1180, 2090
BalI	TGGCCA	2 cut(s)	1071, 1292
BanII	GRGCYC	1 cut(s)	641
BarI	GAAGNNNNNNTAC	2 cut(s)	1789, 1821
BauI	CACGAG	1 cut(s)	2007
BbsI	GAAGAC	1 cut(s)	833
BbvCI	CCTCAGC	1 cut(s)	353
BbvI	GCAGC	5 cut(s)	745, 1204, 1255, 1480, 1483
BccI	CCATC	4 cut(s)	262, 905, 1378, 2066
BceAI	ACGGC	2 cut(s)	384, 1500
BciT130I	CCWGG	6 cut(s)	62, 694, 1109, 1815, 2094, 2210
BciVI	GTATCC	1 cut(s)	916
BclI	TGATCA	3 cut(s)	964, 1615, 2101
BcoDI	GTCTC	3 cut(s)	608, 855, 2224
BfaI	CTAG	3 cut(s)	1761, 1853, 1974
BfmI	CTRYAG	1 cut(s)	981
BfoI	RGCGCY	1 cut(s)	1679
BfuAI	ACCTGC	1 cut(s)	640
BfuI	GTATCC	1 cut(s)	916
BglII	AGATCT	1 cut(s)	1235
BisI	GCNGC	9 cut(s)	91, 275, 759, 762, 816, 1218, 1269, 1494, 1497
BlsI	GCNGC	9 cut(s)	92, 276, 760, 763, 817, 1219, 1270, 1495, 1498
Bme1390I	CCNGG	6 cut(s)	62, 694, 1109, 1815, 2094, 2210
Bme18I	GGWCC	4 cut(s)	279, 424, 1180, 2090
BmeT110I	CYCGRG	1 cut(s)	431
BmgBI	CACGTC	1 cut(s)	175
BmgT120I	GGNCC	7 cut(s)	279, 424, 429, 1180, 1513, 2090, 2212
BmiI	GGNNCC	5 cut(s)	640, 1181, 1182, 1514, 2091
BmrFI	CCNGG	6 cut(s)	62, 694, 1109, 1815, 2094, 2210
BmrI	ACTGGG	3 cut(s)	95, 1179, 1317
BmsI	GCATC	3 cut(s)	597, 940, 1747
BmuI	ACTGGG	3 cut(s)	95, 1179, 1317
BpiI	GAAGAC	1 cut(s)	833
BpmI	CTGGAG	2 cut(s)	182, 1350
Bpu10I	CCTNAGC	1 cut(s)	353
BpuEI	CTTGAG	4 cut(s)	244, 1168, 1193, 1523
Bsa29I	ATCGAT	1 cut(s)	1602
BsaBI	GATNNNNATC	1 cut(s)	963
BsaI	GGTCTC	1 cut(s)	855
BsaJI	CCNNGG	5 cut(s)	442, 621, 693, 1108, 2208
BsaWI	WCCGGW	1 cut(s)	292
BsaXI	ACNNNNNCTCC	4 cut(s)	263, 293, 1089, 1119
Bsc4I	CCNNNNNNNGG	4 cut(s)	348, 1307, 1510, 1511
Bse118I	RCCGGY	1 cut(s)	719
Bse1I	ACTGG	4 cut(s)	90, 390, 1185, 1312
Bse3DI	GCAATG	4 cut(s)	1050, 1425, 1881, 2032
Bse8I	GATNNNNATC	1 cut(s)	963
BseAI	TCCGGA	1 cut(s)	292
BseBI	CCWGG	6 cut(s)	62, 694, 1109, 1815, 2094, 2210
BseCI	ATCGAT	1 cut(s)	1602
BseDI	CCNNGG	5 cut(s)	442, 621, 693, 1108, 2208
BseGI	GGATG	4 cut(s)	301, 612, 664, 955
BseJI	GATNNNNATC	1 cut(s)	963
BseLI	CCNNNNNNNGG	4 cut(s)	348, 1307, 1510, 1511
BseMI	GCAATG	4 cut(s)	1050, 1425, 1881, 2032
BseMII	CTCAG	5 cut(s)	344, 501, 1807, 1887, 2219
BseNI	ACTGG	4 cut(s)	90, 390, 1185, 1312
BseRI	GAGGAG	3 cut(s)	1167, 1252, 1551
BseXI	GCAGC	5 cut(s)	745, 1204, 1255, 1480, 1483
BseYI	CCCAGC	2 cut(s)	798, 818
BsgI	GTGCAG	1 cut(s)	1683
Bsh1236I	CGCG	2 cut(s)	251, 287
BshVI	ATCGAT	1 cut(s)	1602
BsiHKCI	CYCGRG	1 cut(s)	431
BsiSI	CCGG	4 cut(s)	293, 427, 720, 765
BslFI	GGGAC	2 cut(s)	460, 1193
BslI	CCNNNNNNNGG	4 cut(s)	348, 1307, 1510, 1511
BsmAI	GTCTC	3 cut(s)	608, 855, 2224
BsmFI	GGGAC	2 cut(s)	460, 1193
BsmI	GAATGC	1 cut(s)	1477
Bso31I	GGTCTC	1 cut(s)	855
BsoBI	CYCGRG	1 cut(s)	431
Bsp1286I	GDGCHC	1 cut(s)	641
Bsp13I	TCCGGA	1 cut(s)	292
BspCNI	CTCAG	5 cut(s)	345, 502, 1806, 1886, 2220
BspDI	ATCGAT	1 cut(s)	1602
BspEI	TCCGGA	1 cut(s)	292
BspFNI	CGCG	2 cut(s)	251, 287
BspHI	TCATGA	3 cut(s)	1132, 1411, 1596
BspLI	GGNNCC	5 cut(s)	640, 1181, 1182, 1514, 2091
BspMAI	CTGCAG	1 cut(s)	985
BspMI	ACCTGC	1 cut(s)	640
BspPI	GGATC	1 cut(s)	1404
BspQI	GCTCTTC	3 cut(s)	188, 981, 1560
BspTNI	GGTCTC	1 cut(s)	855
BsrBI	CCGCTC	1 cut(s)	274
BsrDI	GCAATG	4 cut(s)	1050, 1425, 1881, 2032
BsrFI	RCCGGY	1 cut(s)	719
BsrI	ACTGG	4 cut(s)	90, 390, 1185, 1312
BssAI	RCCGGY	1 cut(s)	719
BssECI	CCNNGG	5 cut(s)	442, 621, 693, 1108, 2208
BssSI	CACGAG	1 cut(s)	2007
BssT1I	CCWWGG	1 cut(s)	621
Bst2BI	CACGAG	1 cut(s)	2007
Bst2UI	CCWGG	6 cut(s)	62, 694, 1109, 1815, 2094, 2210
Bst4CI	ACNGT	6 cut(s)	709, 727, 1063, 1288, 1843, 2063
Bst6I	CTCTTC	5 cut(s)	188, 981, 1560, 1665, 1792
BstAPI	GCANNNNNTGC	1 cut(s)	1362
BstC8I	GCNNGC	4 cut(s)	253, 637, 1089, 1344
BstDEI	CTNAG	6 cut(s)	353, 510, 1679, 1793, 1873, 2228
BstDSI	CCRYGG	1 cut(s)	442
BstEII	GGTNACC	1 cut(s)	2203
BstENI	CCTNNNNNAGG	1 cut(s)	346
BstF5I	GGATG	4 cut(s)	301, 612, 664, 955
BstFNI	CGCG	2 cut(s)	251, 287
BstH2I	RGCGCY	1 cut(s)	1679
BstHHI	GCGC	3 cut(s)	542, 804, 1678
BstMAI	GTCTC	3 cut(s)	608, 855, 2224
BstMWI	GCNNNNNNNGC	9 cut(s)	96, 134, 539, 548, 557, 758, 887, 1289, 1362
BstNI	CCWGG	6 cut(s)	62, 694, 1109, 1815, 2094, 2210
BstPI	GGTNACC	1 cut(s)	2203
BstSCI	CCNGG	6 cut(s)	60, 692, 1107, 1813, 2092, 2208
BstSFI	CTRYAG	1 cut(s)	981
BstUI	CGCG	2 cut(s)	251, 287
BstV1I	GCAGC	5 cut(s)	745, 1204, 1255, 1480, 1483
BstV2I	GAAGAC	1 cut(s)	833
BstX2I	RGATCY	1 cut(s)	1235
BstXI	CCANNNNNNTGG	1 cut(s)	655
BstYI	RGATCY	1 cut(s)	1235
Bsu15I	ATCGAT	1 cut(s)	1602
BsuI	GTATCC	1 cut(s)	916
BsuTUI	ATCGAT	1 cut(s)	1602
BtgI	CCRYGG	1 cut(s)	442
BtrI	CACGTC	1 cut(s)	175
BtsCI	GGATG	4 cut(s)	301, 612, 664, 955
BtsI	GCAGTG	2 cut(s)	978, 1941
BtsIMutI	CAGTG	5 cut(s)	507, 732, 978, 1466, 1941
BveI	ACCTGC	1 cut(s)	640
Cac8I	GCNNGC	4 cut(s)	253, 637, 1089, 1344
CaiI	CAGNNNCTG	2 cut(s)	1307, 2150
CciI	TCATGA	3 cut(s)	1132, 1411, 1596
CfoI	GCGC	3 cut(s)	542, 804, 1678
Cfr10I	RCCGGY	1 cut(s)	719
Cfr13I	GGNCC	7 cut(s)	279, 424, 429, 1180, 1513, 2090, 2212
ClaI	ATCGAT	1 cut(s)	1602
CseI	GACGC	1 cut(s)	2213
Csp6I	GTAC	5 cut(s)	505, 602, 1655, 2052, 2085
CviQI	GTAC	5 cut(s)	505, 602, 1655, 2052, 2085
DdeI	CTNAG	6 cut(s)	353, 510, 1679, 1793, 1873, 2228
EaeI	YGGCCR	2 cut(s)	1069, 1290
Eam1104I	CTCTTC	5 cut(s)	188, 981, 1560, 1665, 1792
EarI	CTCTTC	5 cut(s)	188, 981, 1560, 1665, 1792
EciI	GGCGGA	1 cut(s)	659
Eco130I	CCWWGG	1 cut(s)	621
Eco24I	GRGCYC	1 cut(s)	641
Eco31I	GGTCTC	1 cut(s)	855
Eco32I	GATATC	2 cut(s)	1081, 1159
Eco47I	GGWCC	4 cut(s)	279, 424, 1180, 2090
Eco57I	CTGAAG	2 cut(s)	64, 1727
Eco88I	CYCGRG	1 cut(s)	431
Eco91I	GGTNACC	1 cut(s)	2203
EcoNI	CCTNNNNNAGG	1 cut(s)	346
EcoO109I	RGGNCCY	2 cut(s)	1180, 2212
EcoO65I	GGTNACC	1 cut(s)	2203
EcoRI	GAATTC	1 cut(s)	892
EcoRII	CCWGG	6 cut(s)	60, 692, 1107, 1813, 2092, 2208
EcoRV	GATATC	2 cut(s)	1081, 1159
EcoT14I	CCWWGG	1 cut(s)	621
EcoT38I	GRGCYC	1 cut(s)	641
ErhI	CCWWGG	1 cut(s)	621
FalI	AAGNNNNNCTT	2 cut(s)	1662, 1694
FaqI	GGGAC	2 cut(s)	460, 1193
FauI	CCCGC	1 cut(s)	628
FauNDI	CATATG	2 cut(s)	1723, 2269
FbaI	TGATCA	3 cut(s)	964, 1615, 2101
Fnu4HI	GCNGC	9 cut(s)	91, 275, 759, 762, 816, 1218, 1269, 1494, 1497
FokI	GGATG	4 cut(s)	308, 619, 671, 962
FriOI	GRGCYC	1 cut(s)	641
Fsp4HI	GCNGC	9 cut(s)	91, 275, 759, 762, 816, 1218, 1269, 1494, 1497
FspBI	CTAG	3 cut(s)	1761, 1853, 1974
FspI	TGCGCA	1 cut(s)	541
GlaI	GCGC	3 cut(s)	541, 803, 1677
GluI	GCNGC	9 cut(s)	91, 275, 759, 762, 816, 1218, 1269, 1494, 1497
GsaI	CCCAGC	2 cut(s)	802, 822
GsuI	CTGGAG	2 cut(s)	182, 1350
HaeII	RGCGCY	1 cut(s)	1679
HapII	CCGG	4 cut(s)	293, 427, 720, 765
HgaI	GACGC	1 cut(s)	2213
HhaI	GCGC	3 cut(s)	542, 804, 1678
Hin6I	GCGC	3 cut(s)	540, 802, 1676
HinP1I	GCGC	3 cut(s)	540, 802, 1676
HincII	GTYRAC	1 cut(s)	304
HindII	GTYRAC	1 cut(s)	304
HindIII	AAGCTT	4 cut(s)	6, 76, 792, 2171
HpaI	GTTAAC	1 cut(s)	304
HpaII	CCGG	4 cut(s)	293, 427, 720, 765
HphI	GGTGA	7 cut(s)	406, 418, 713, 959, 1814, 2075, 2108
Hpy166II	GTNNAC	9 cut(s)	28, 147, 304, 507, 978, 1120, 1128, 1243, 1777
Hpy188III	TCNNGA	8 cut(s)	49, 199, 223, 293, 1133, 1412, 1597, 2009
Hpy8I	GTNNAC	9 cut(s)	28, 147, 304, 507, 978, 1120, 1128, 1243, 1777
Hpy99I	CGWCG	2 cut(s)	218, 370
HpyAV	CCTTC	9 cut(s)	28, 89, 196, 334, 653, 682, 821, 940, 1130
HpyCH4III	ACNGT	6 cut(s)	709, 727, 1063, 1288, 1843, 2063
HpyCH4IV	ACGT	3 cut(s)	174, 1389, 1548
HpyF10VI	GCNNNNNNNGC	9 cut(s)	96, 134, 539, 548, 557, 758, 887, 1289, 1362
HpyF3I	CTNAG	6 cut(s)	353, 510, 1679, 1793, 1873, 2228
HpySE526I	ACGT	3 cut(s)	174, 1389, 1548
HspAI	GCGC	3 cut(s)	540, 802, 1676
KflI	GGGWCCC	1 cut(s)	1180
Kpn2I	TCCGGA	1 cut(s)	292
Ksp22I	TGATCA	3 cut(s)	964, 1615, 2101
KspAI	GTTAAC	1 cut(s)	304
LguI	GCTCTTC	3 cut(s)	188, 981, 1560
LmnI	GCTCC	5 cut(s)	271, 644, 1110, 1331, 1816
Lsp1109I	GCAGC	5 cut(s)	745, 1204, 1255, 1480, 1483
LweI	GCATC	3 cut(s)	597, 940, 1747
MaeI	CTAG	3 cut(s)	1761, 1853, 1974
MaeII	ACGT	3 cut(s)	174, 1389, 1548
MaeIII	GTNAC	4 cut(s)	406, 701, 2009, 2203
MbiI	CCGCTC	1 cut(s)	274
MflI	RGATCY	1 cut(s)	1235
MhlI	GDGCHC	1 cut(s)	641
MlsI	TGGCCA	2 cut(s)	1071, 1292
MluI	ACGCGT	1 cut(s)	249
MluNI	TGGCCA	2 cut(s)	1071, 1292
MlyI	GAGTC	6 cut(s)	152, 403, 840, 1772, 1863, 1979
MmeI	TCCRAC	2 cut(s)	237, 306
Mox20I	TGGCCA	2 cut(s)	1071, 1292
MroI	TCCGGA	1 cut(s)	292
MroXI	GAANNNNTTC	2 cut(s)	56, 2193
MscI	TGGCCA	2 cut(s)	1071, 1292
MseI	TTAA	8 cut(s)	303, 453, 480, 1277, 1337, 1748, 1862, 1911
MslI	CAYNNNNRTG	1 cut(s)	152
Msp20I	TGGCCA	2 cut(s)	1071, 1292
MspA1I	CMGCKG	1 cut(s)	1705
MspI	CCGG	4 cut(s)	293, 427, 720, 765
MspR9I	CCNGG	6 cut(s)	62, 694, 1109, 1815, 2094, 2210
Mva1269I	GAATGC	1 cut(s)	1477
MvaI	CCWGG	6 cut(s)	62, 694, 1109, 1815, 2094, 2210
MvnI	CGCG	2 cut(s)	251, 287
MwoI	GCNNNNNNNGC	9 cut(s)	96, 134, 539, 548, 557, 758, 887, 1289, 1362
NdeI	CATATG	2 cut(s)	1723, 2269
NlaIV	GGNNCC	5 cut(s)	640, 1181, 1182, 1514, 2091
NmeAIII	GCCGAG	1 cut(s)	142
NmuCI	GTSAC	3 cut(s)	406, 701, 2009
NsbI	TGCGCA	1 cut(s)	541
PagI	TCATGA	3 cut(s)	1132, 1411, 1596
PciSI	GCTCTTC	3 cut(s)	188, 981, 1560
PcsI	WCGNNNNNNNCGW	1 cut(s)	2067
PctI	GAATGC	1 cut(s)	1477
PdmI	GAANNNNTTC	2 cut(s)	56, 2193
PfeI	GAWTC	4 cut(s)	18, 289, 1903, 2003
PflFI	GACNNNGTC	1 cut(s)	707
PflMI	CCANNNNNTGG	1 cut(s)	1307
PfoI	TCCNGGA	1 cut(s)	60
PkrI	GCNGC	9 cut(s)	92, 276, 760, 763, 817, 1219, 1270, 1495, 1498
PleI	GAGTC	6 cut(s)	151, 403, 839, 1772, 1863, 1978
PpsI	GAGTC	6 cut(s)	151, 403, 839, 1772, 1863, 1978
PpuMI	RGGWCCY	1 cut(s)	1180
PshBI	ATTAAT	2 cut(s)	1337, 1862
PsiI	TTATAA	2 cut(s)	665, 2177
Psp5II	RGGWCCY	1 cut(s)	1180
Psp6I	CCWGG	6 cut(s)	60, 692, 1107, 1813, 2092, 2208
PspEI	GGTNACC	1 cut(s)	2203
PspFI	CCCAGC	2 cut(s)	798, 818
PspGI	CCWGG	6 cut(s)	60, 692, 1107, 1813, 2092, 2208
PspN4I	GGNNCC	5 cut(s)	640, 1181, 1182, 1514, 2091
PspPI	GGNCC	7 cut(s)	279, 424, 429, 1180, 1513, 2090, 2212
PspPPI	RGGWCCY	1 cut(s)	1180
PstI	CTGCAG	1 cut(s)	985
PstNI	CAGNNNCTG	2 cut(s)	1307, 2150
PsuI	RGATCY	1 cut(s)	1235
PsyI	GACNNNGTC	1 cut(s)	707
PvuII	CAGCTG	1 cut(s)	1705
RsaI	GTAC	5 cut(s)	506, 603, 1656, 2053, 2086
RsaNI	GTAC	5 cut(s)	505, 602, 1655, 2052, 2085
RseI	CAYNNNNRTG	1 cut(s)	152
SapI	GCTCTTC	3 cut(s)	188, 981, 1560
SaqAI	TTAA	8 cut(s)	303, 453, 480, 1277, 1337, 1748, 1862, 1911
SatI	GCNGC	9 cut(s)	91, 275, 759, 762, 816, 1218, 1269, 1494, 1497
Sau96I	GGNCC	7 cut(s)	279, 424, 429, 1180, 1513, 2090, 2212
SchI	GAGTC	6 cut(s)	152, 403, 840, 1772, 1863, 1979
ScrFI	CCNGG	6 cut(s)	62, 694, 1109, 1815, 2094, 2210
SduI	GDGCHC	1 cut(s)	641
SfaNI	GCATC	3 cut(s)	597, 940, 1747
SfcI	CTRYAG	1 cut(s)	981
SinI	GGWCC	4 cut(s)	279, 424, 1180, 2090
SmiMI	CAYNNNNRTG	1 cut(s)	152
SmlI	CTYRAG	4 cut(s)	223, 1147, 1208, 1538
SmoI	CTYRAG	4 cut(s)	223, 1147, 1208, 1538
SspI	AATATT	1 cut(s)	2192
SspMI	CTAG	3 cut(s)	1761, 1853, 1974
StyD4I	CCNGG	6 cut(s)	60, 692, 1107, 1813, 2092, 2208
StyI	CCWWGG	1 cut(s)	621
TaaI	ACNGT	6 cut(s)	709, 727, 1063, 1288, 1843, 2063
TaiI	ACGT	3 cut(s)	177, 1392, 1551
TaqI	TCGA	7 cut(s)	48, 123, 162, 844, 1477, 1602, 1888
TatI	WGTACW	2 cut(s)	504, 1654
TauI	GCSGC	4 cut(s)	93, 277, 764, 818
TfiI	GAWTC	4 cut(s)	18, 289, 1903, 2003
Tru1I	TTAA	8 cut(s)	303, 453, 480, 1277, 1337, 1748, 1862, 1911
Tru9I	TTAA	8 cut(s)	303, 453, 480, 1277, 1337, 1748, 1862, 1911
TscAI	CASTG	5 cut(s)	514, 732, 985, 1473, 1941
TseFI	GTSAC	3 cut(s)	406, 701, 2009
TseI	GCWGC	5 cut(s)	758, 1217, 1268, 1493, 1496
Tsp45I	GTSAC	3 cut(s)	406, 701, 2009
TspDTI	ATGAA	8 cut(s)	148, 840, 1011, 1149, 1386, 1400, 2202, 2278
TspGWI	ACGGA	3 cut(s)	619, 949, 1916
TspRI	CASTG	5 cut(s)	514, 732, 985, 1473, 1941
Tth111I	GACNNNGTC	1 cut(s)	707
Van91I	CCANNNNNTGG	1 cut(s)	1307
VpaK11BI	GGWCC	4 cut(s)	279, 424, 1180, 2090
VspI	ATTAAT	2 cut(s)	1337, 1862
XagI	CCTNNNNNAGG	1 cut(s)	346
XapI	RAATTY	5 cut(s)	581, 892, 1313, 1373, 1750
XcmI	CCANNNNNNNNNTGG	1 cut(s)	806
XmnI	GAANNNNTTC	2 cut(s)	56, 2193
XspI	CTAG	3 cut(s)	1761, 1853, 1974

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.