MD05G1249200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0014463
Physical Location & Seq
Forward (+)
38083518 .. 38083901
384 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1249200.v1.1.491

Sequence Viewer

Length: 384 bp
ATGTGGGTTATTTCTTGGCCCAAAACCACTTGCCCCACCAAGACCGGTGGCCGAGAAGGTGGCACCACCACAGCCACCGCCGCCGCTGCAGCTACCAGTTGGTGTATCAATGTTTCATCATCCCACTGCACTTGTCTAACCAAACTGGTGACGAAGCTAAGGAAACATAGGAGAAGGGTAAGGGCTGCAGCTAGTAGGCGACGTTCACTGCAGTGCCACTATGATCCACTGAGCTATTCTCTCAACTTTGATGCCAGTGGATGTGGGAACTTGTTGGATGACCAAGATTACTTCAAGTTCTGTGCATTTTCCTCCAGGTTTGTGGCCAACCCTTCAGGAAACTCAGCTTCTACTCCAAGGCTAATCACCACCACTCCTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

128

Amino Acids

13.91

Weight (kDa)

9.86

Isoelectric Point (pI)

53.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014463)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19980
fragaria_vesca FvH4_3g12620
malus_domestica MD05G1249200.v1.1 MD10G1229700.v1.1
prunus_persica Prupe.4G115000_v2.0.a1
pyrus_communis pycom05g22630 pycom10g19320
rosa_chinensis RchiOBHm_Chr5g0020591
rosa_laevigata RLG00000032540
rosa_multiflora Rmu_sc0005607.1_g000006
rosa_roxburghii Rroxscaffold_1G00057700
rosa_rugosa Rorug05G0061500
rosa_samantha Rh5AG151400 Rh5BG150900 Rh5CG162300 Rh5DG150300
rosa_wichuraiana Rw5G013400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 62
AciI CCGC 3 cut(s) 78, 81, 84
AclWI GGATC 1 cut(s) 218
AcoI YGGCCR 2 cut(s) 49, 324
AcuI CTGAAG 1 cut(s) 318
AgeI ACCGGT 1 cut(s) 44
AgsI TTSAA 1 cut(s) 295
AjnI CCWGG 1 cut(s) 314
AleI CACNNNNGTG 1 cut(s) 211
AluBI AGCT 5 cut(s) 92, 157, 191, 234, 347
AluI AGCT 5 cut(s) 92, 157, 191, 234, 347
AlwI GGATC 1 cut(s) 218
AoxI GGCC 3 cut(s) 17, 49, 324
ApeKI GCWGC 4 cut(s) 86, 89, 185, 188
AsiGI ACCGGT 1 cut(s) 44
AspS9I GGNCC 1 cut(s) 18
AsuHPI GGTGA 2 cut(s) 160, 358
BalI TGGCCA 1 cut(s) 326
BanI GGYRCC 1 cut(s) 62
BbvI GCAGC 4 cut(s) 73, 101, 172, 200
BciT130I CCWGG 1 cut(s) 316
BfaI CTAG 2 cut(s) 192, 382
BfmI CTRYAG 3 cut(s) 87, 186, 209
BisI GCNGC 6 cut(s) 81, 84, 87, 90, 186, 189
BlsI GCNGC 6 cut(s) 82, 85, 88, 91, 187, 190
Bme1390I CCNGG 1 cut(s) 316
BmgT120I GGNCC 1 cut(s) 18
BmiI GGNNCC 1 cut(s) 64
BmrFI CCNGG 1 cut(s) 316
BmsI GCATC 1 cut(s) 241
BplI GAGNNNNNCTC 2 cut(s) 223, 255
BpmI CTGGAG 1 cut(s) 298
Bpu10I CCTNAGC 1 cut(s) 158
BsaJI CCNNGG 1 cut(s) 356
BsaWI WCCGGW 1 cut(s) 44
BsaXI ACNNNNNCTCC 1 cut(s) 358
Bse118I RCCGGY 1 cut(s) 44
Bse1I ACTGG 3 cut(s) 96, 150, 255
BseBI CCWGG 1 cut(s) 316
BseDI CCNNGG 1 cut(s) 356
BseGI GGATG 3 cut(s) 119, 266, 283
BseMII CTCAG 2 cut(s) 221, 357
BseNI ACTGG 3 cut(s) 96, 150, 255
BseRI GAGGAG 1 cut(s) 366
BseXI GCAGC 4 cut(s) 73, 101, 172, 200
BsgI GTGCAG 1 cut(s) 112
BshFI GGCC 3 cut(s) 19, 51, 326
BshNI GGYRCC 1 cut(s) 62
BshTI ACCGGT 1 cut(s) 44
BsiSI CCGG 1 cut(s) 45
BsnI GGCC 3 cut(s) 19, 51, 326
Bsp143I GATC 1 cut(s) 223
BspACI CCGC 3 cut(s) 78, 81, 84
BspANI GGCC 3 cut(s) 19, 51, 326
BspCNI CTCAG 2 cut(s) 222, 356
BspLI GGNNCC 1 cut(s) 64
BspMAI CTGCAG 3 cut(s) 91, 190, 213
BspPI GGATC 1 cut(s) 218
BspT107I GGYRCC 1 cut(s) 62
BsrFI RCCGGY 1 cut(s) 44
BsrI ACTGG 3 cut(s) 96, 150, 255
BssAI RCCGGY 1 cut(s) 44
BssECI CCNNGG 1 cut(s) 356
BssMI GATC 1 cut(s) 223
BssT1I CCWWGG 1 cut(s) 356
Bst2UI CCWGG 1 cut(s) 316
BstDEI CTNAG 3 cut(s) 158, 230, 343
BstF5I GGATG 3 cut(s) 119, 266, 283
BstKTI GATC 1 cut(s) 226
BstMBI GATC 1 cut(s) 223
BstMWI GCNNNNNNNGC 3 cut(s) 80, 86, 89
BstNI CCWGG 1 cut(s) 316
BstSCI CCNGG 1 cut(s) 314
BstSFI CTRYAG 3 cut(s) 87, 186, 209
BstV1I GCAGC 4 cut(s) 73, 101, 172, 200
BstXI CCANNNNNNTGG 1 cut(s) 322
BsuRI GGCC 3 cut(s) 19, 51, 326
BtsCI GGATG 3 cut(s) 119, 266, 283
BtsI GCAGTG 3 cut(s) 124, 206, 218
BtsIMutI CAGTG 5 cut(s) 124, 206, 218, 227, 262
Cfr10I RCCGGY 1 cut(s) 44
Cfr13I GGNCC 1 cut(s) 18
CspAI ACCGGT 1 cut(s) 44
CspCI CAANNNNNGTGG 4 cut(s) 28, 63, 113, 148
DdeI CTNAG 3 cut(s) 158, 230, 343
DpnI GATC 1 cut(s) 225
DpnII GATC 1 cut(s) 223
EaeI YGGCCR 2 cut(s) 49, 324
Eco130I CCWWGG 1 cut(s) 356
Eco57I CTGAAG 1 cut(s) 318
EcoRII CCWGG 1 cut(s) 314
EcoT14I CCWWGG 1 cut(s) 356
ErhI CCWWGG 1 cut(s) 356
FaiI YATR 2 cut(s) 168, 222
Fnu4HI GCNGC 6 cut(s) 81, 84, 87, 90, 186, 189
FokI GGATG 3 cut(s) 106, 273, 290
Fsp4HI GCNGC 6 cut(s) 81, 84, 87, 90, 186, 189
FspBI CTAG 2 cut(s) 192, 382
GluI GCNGC 6 cut(s) 81, 84, 87, 90, 186, 189
GsuI CTGGAG 1 cut(s) 298
HaeIII GGCC 3 cut(s) 19, 51, 326
HapII CCGG 1 cut(s) 45
HpaII CCGG 1 cut(s) 45
HphI GGTGA 2 cut(s) 160, 358
Hpy166II GTNNAC 1 cut(s) 206
Hpy188III TCNNGA 1 cut(s) 336
Hpy8I GTNNAC 1 cut(s) 206
Hpy99I CGWCG 1 cut(s) 204
HpyAV CCTTC 3 cut(s) 50, 168, 342
HpyCH4IV ACGT 1 cut(s) 202
HpyCH4V TGCA 5 cut(s) 89, 129, 188, 211, 305
HpyF10VI GCNNNNNNNGC 3 cut(s) 80, 86, 89
HpyF3I CTNAG 3 cut(s) 158, 230, 343
HpySE526I ACGT 1 cut(s) 202
Kzo9I GATC 1 cut(s) 223
LpnPI CCDG 7 cut(s) 58, 109, 131, 268, 301, 321, 328
Lsp1109I GCAGC 4 cut(s) 73, 101, 172, 200
LweI GCATC 1 cut(s) 241
MaeI CTAG 2 cut(s) 192, 382
MaeII ACGT 1 cut(s) 202
MaeIII GTNAC 1 cut(s) 148
MalI GATC 1 cut(s) 225
MboI GATC 1 cut(s) 223
MlsI TGGCCA 1 cut(s) 326
MluNI TGGCCA 1 cut(s) 326
MmeI TCCRAC 1 cut(s) 255
MnlI CCTC 1 cut(s) 322
Mox20I TGGCCA 1 cut(s) 326
MscI TGGCCA 1 cut(s) 326
MslI CAYNNNNRTG 1 cut(s) 211
Msp20I TGGCCA 1 cut(s) 326
MspA1I CMGCKG 1 cut(s) 86
MspI CCGG 1 cut(s) 45
MspR9I CCNGG 1 cut(s) 316
MvaI CCWGG 1 cut(s) 316
MwoI GCNNNNNNNGC 3 cut(s) 80, 86, 89
NdeII GATC 1 cut(s) 223
NlaIV GGNNCC 1 cut(s) 64
NmeAIII GCCGAG 1 cut(s) 77
NmuCI GTSAC 1 cut(s) 148
OliI CACNNNNGTG 1 cut(s) 211
PinAI ACCGGT 1 cut(s) 44
PkrI GCNGC 6 cut(s) 82, 85, 88, 91, 187, 190
Psp6I CCWGG 1 cut(s) 314
PspGI CCWGG 1 cut(s) 314
PspN4I GGNNCC 1 cut(s) 64
PspPI GGNCC 1 cut(s) 18
PsrI GAACNNNNNNTAC 2 cut(s) 187, 219
PstI CTGCAG 3 cut(s) 91, 190, 213
RseI CAYNNNNRTG 1 cut(s) 211
SatI GCNGC 6 cut(s) 81, 84, 87, 90, 186, 189
Sau3AI GATC 1 cut(s) 223
Sau96I GGNCC 1 cut(s) 18
ScrFI CCNGG 1 cut(s) 316
SetI ASST 8 cut(s) 61, 94, 159, 193, 205, 236, 320, 349
SfaNI GCATC 1 cut(s) 241
SfcI CTRYAG 3 cut(s) 87, 186, 209
SmiMI CAYNNNNRTG 1 cut(s) 211
SsiI CCGC 3 cut(s) 78, 81, 84
SspMI CTAG 2 cut(s) 192, 382
StyD4I CCNGG 1 cut(s) 314
StyI CCWWGG 1 cut(s) 356
TaiI ACGT 1 cut(s) 205
TauI GCSGC 2 cut(s) 83, 86
TscAI CASTG 5 cut(s) 131, 213, 218, 234, 262
TseFI GTSAC 1 cut(s) 148
TseI GCWGC 4 cut(s) 86, 89, 185, 188
Tsp45I GTSAC 1 cut(s) 148
TspDTI ATGAA 1 cut(s) 105
TspRI CASTG 5 cut(s) 131, 213, 218, 234, 262
XspI CTAG 2 cut(s) 192, 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.