pycom05g22630

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0014463
Physical Location & Seq
Forward (+)
24886554 .. 24887373
820 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g22630.3

Sequence Viewer

Length: 732 bp
ATGATTAAACCAATAAAATTACTCATACAGTTTGGTCATCAAACTATAAAATTATTATCCTCTAACACGCATAAAACAGTAAAAATGAACCGAGATTTAGAATTTAGGAACCTCCGAATTTGGTGGATGATACATTTTTTATACATTGAAAAGTACCACAAAACCATTCAAAAATTTGGCTTTGCATGCAACTTTTTTTTTAATTTATCTTTTTTTTTTTTTTTTTTTTTTTTAACTTTTAAAACCAAACTCACTTCCATCACCACATCATTCGCTGTAATCATTTTCAACTCGCTAACTCCAAATTTCTCAAAAAAACTAGAGAAGTTCCATATTTTCACAATGTGGCTTATTTCTTGGCCCAAAACCACTTGCCCCACCAAGACCGGTGGAGGAGAAGGTGGCACCACCACAGCCACCGCCGCTGCCGCCGCTGCAGCCGCCAGTTGGTGTATCAATGTTTCATCATCCCACTGCACTTGTCTAAGCAAACTGGTGGGGAAGCTAAGGAAACATAGGAGAAGGGTAAGGGCTGCAGCTAGTAGGCAACGTTCACTGCAGTGCCACTATGATCCACTGAGCTATTCTCTTAACTTTGATGCCAGTGGATGTGGGAACTTGTTGGATGACCAAGATTACTTCAAGTTCTGTGCATTTTCCTCCAGGTTTGTGGCCAACCCCTCAGGAAACTCAGCTTCTACTCCAAGGCTAATCACCACCACTCCTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

244

Amino Acids

27.72

Weight (kDa)

10.04

Isoelectric Point (pI)

36.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014463)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19980
fragaria_vesca FvH4_3g12620
malus_domestica MD05G1249200.v1.1 MD10G1229700.v1.1
prunus_persica Prupe.4G115000_v2.0.a1
pyrus_communis pycom05g22630 pycom10g19320
rosa_chinensis RchiOBHm_Chr5g0020591
rosa_laevigata RLG00000032540
rosa_multiflora Rmu_sc0005607.1_g000006
rosa_roxburghii Rroxscaffold_1G00057700
rosa_rugosa Rorug05G0061500
rosa_samantha Rh5AG151400 Rh5BG150900 Rh5CG162300 Rh5DG150300
rosa_wichuraiana Rw5G013400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 404
AciI CCGC 5 cut(s) 420, 423, 429, 432, 441
AclI AACGTT 1 cut(s) 550
AclWI GGATC 1 cut(s) 566
AcoI YGGCCR 1 cut(s) 672
AcsI RAATTY 4 cut(s) 101, 117, 173, 304
AdeI CACNNNGTG 1 cut(s) 345
AfaI GTAC 1 cut(s) 155
AfiI CCNNNNNNNGG 1 cut(s) 447
AgeI ACCGGT 1 cut(s) 386
AgsI TTSAA 4 cut(s) 149, 170, 289, 643
AjnI CCWGG 1 cut(s) 662
AleI CACNNNNGTG 1 cut(s) 559
AluBI AGCT 4 cut(s) 505, 539, 582, 695
AluI AGCT 4 cut(s) 505, 539, 582, 695
AlwI GGATC 1 cut(s) 566
AoxI GGCC 2 cut(s) 359, 672
ApeKI GCWGC 5 cut(s) 425, 434, 437, 533, 536
ApoI RAATTY 4 cut(s) 101, 117, 173, 304
AsiGI ACCGGT 1 cut(s) 386
AspS9I GGNCC 1 cut(s) 360
AsuHPI GGTGA 2 cut(s) 253, 706
AxyI CCTNAGG 1 cut(s) 682
BalI TGGCCA 1 cut(s) 674
BanI GGYRCC 1 cut(s) 404
BbvI GCAGC 5 cut(s) 412, 421, 449, 520, 548
BccI CCATC 1 cut(s) 266
BciT130I CCWGG 1 cut(s) 664
BfaI CTAG 3 cut(s) 320, 540, 730
BfmI CTRYAG 3 cut(s) 435, 534, 557
BisI GCNGC 9 cut(s) 423, 426, 429, 432, 435, 438, 441, 534, 537
BlsI GCNGC 9 cut(s) 424, 427, 430, 433, 436, 439, 442, 535, 538
Bme1390I CCNGG 1 cut(s) 664
BmgT120I GGNCC 1 cut(s) 360
BmiI GGNNCC 2 cut(s) 110, 406
BmrFI CCNGG 1 cut(s) 664
BmsI GCATC 1 cut(s) 589
BplI GAGNNNNNCTC 2 cut(s) 571, 603
BpmI CTGGAG 1 cut(s) 646
Bpu10I CCTNAGC 1 cut(s) 506
BsaJI CCNNGG 1 cut(s) 704
BsaWI WCCGGW 1 cut(s) 386
BsaXI ACNNNNNCTCC 1 cut(s) 706
Bsc4I CCNNNNNNNGG 1 cut(s) 447
Bse118I RCCGGY 1 cut(s) 386
Bse1I ACTGG 3 cut(s) 444, 498, 603
Bse21I CCTNAGG 1 cut(s) 682
BseBI CCWGG 1 cut(s) 664
BseDI CCNNGG 1 cut(s) 704
BseGI GGATG 4 cut(s) 132, 467, 614, 631
BseLI CCNNNNNNNGG 1 cut(s) 447
BseMII CTCAG 3 cut(s) 569, 696, 705
BseNI ACTGG 3 cut(s) 444, 498, 603
BseRI GAGGAG 2 cut(s) 408, 714
BseXI GCAGC 5 cut(s) 412, 421, 449, 520, 548
BsgI GTGCAG 1 cut(s) 460
BshFI GGCC 2 cut(s) 361, 674
BshNI GGYRCC 1 cut(s) 404
BshTI ACCGGT 1 cut(s) 386
BsiSI CCGG 1 cut(s) 387
BslI CCNNNNNNNGG 1 cut(s) 447
BsnI GGCC 2 cut(s) 361, 674
Bsp143I GATC 1 cut(s) 571
BspACI CCGC 5 cut(s) 420, 423, 429, 432, 441
BspANI GGCC 2 cut(s) 361, 674
BspCNI CTCAG 3 cut(s) 570, 695, 704
BspLI GGNNCC 2 cut(s) 110, 406
BspMAI CTGCAG 3 cut(s) 439, 538, 561
BspPI GGATC 1 cut(s) 566
BspT107I GGYRCC 1 cut(s) 404
BsrFI RCCGGY 1 cut(s) 386
BsrI ACTGG 3 cut(s) 444, 498, 603
BssAI RCCGGY 1 cut(s) 386
BssECI CCNNGG 1 cut(s) 704
BssMI GATC 1 cut(s) 571
BssT1I CCWWGG 1 cut(s) 704
Bst2UI CCWGG 1 cut(s) 664
Bst4CI ACNGT 2 cut(s) 30, 79
BstC8I GCNNGC 1 cut(s) 187
BstDEI CTNAG 5 cut(s) 485, 506, 578, 682, 691
BstF5I GGATG 4 cut(s) 132, 467, 614, 631
BstKTI GATC 1 cut(s) 574
BstMBI GATC 1 cut(s) 571
BstMWI GCNNNNNNNGC 7 cut(s) 186, 422, 428, 431, 434, 437, 440
BstNI CCWGG 1 cut(s) 664
BstNSI RCATGY 1 cut(s) 189
BstSCI CCNGG 1 cut(s) 662
BstSFI CTRYAG 3 cut(s) 435, 534, 557
BstV1I GCAGC 5 cut(s) 412, 421, 449, 520, 548
BstXI CCANNNNNNTGG 1 cut(s) 670
Bsu36I CCTNAGG 1 cut(s) 682
BsuRI GGCC 2 cut(s) 361, 674
BtsCI GGATG 4 cut(s) 132, 467, 614, 631
BtsI GCAGTG 3 cut(s) 472, 554, 566
BtsIMutI CAGTG 5 cut(s) 472, 554, 566, 575, 610
Cac8I GCNNGC 1 cut(s) 187
Cfr10I RCCGGY 1 cut(s) 386
Cfr13I GGNCC 1 cut(s) 360
Csp6I GTAC 1 cut(s) 154
CspAI ACCGGT 1 cut(s) 386
CspCI CAANNNNNGTGG 4 cut(s) 370, 405, 461, 496
CviAII CATG 1 cut(s) 186
CviQI GTAC 1 cut(s) 154
DdeI CTNAG 5 cut(s) 485, 506, 578, 682, 691
DpnI GATC 1 cut(s) 573
DpnII GATC 1 cut(s) 571
DraI TTTAAA 1 cut(s) 241
DraIII CACNNNGTG 1 cut(s) 345
EaeI YGGCCR 1 cut(s) 672
Eco130I CCWWGG 1 cut(s) 704
Eco81I CCTNAGG 1 cut(s) 682
EcoRII CCWGG 1 cut(s) 662
EcoT14I CCWWGG 1 cut(s) 704
ErhI CCWWGG 1 cut(s) 704
FaeI CATG 1 cut(s) 189
FaiI YATR 8 cut(s) 26, 47, 72, 142, 187, 333, 516, 570
FatI CATG 1 cut(s) 185
Fnu4HI GCNGC 9 cut(s) 423, 426, 429, 432, 435, 438, 441, 534, 537
FokI GGATG 4 cut(s) 139, 454, 621, 638
Fsp4HI GCNGC 9 cut(s) 423, 426, 429, 432, 435, 438, 441, 534, 537
FspBI CTAG 3 cut(s) 320, 540, 730
GluI GCNGC 9 cut(s) 423, 426, 429, 432, 435, 438, 441, 534, 537
GsuI CTGGAG 1 cut(s) 646
HaeIII GGCC 2 cut(s) 361, 674
HapII CCGG 1 cut(s) 387
Hin1II CATG 1 cut(s) 189
HpaII CCGG 1 cut(s) 387
HphI GGTGA 2 cut(s) 253, 706
Hpy166II GTNNAC 1 cut(s) 554
Hpy188I TCNGA 1 cut(s) 116
Hpy188III TCNNGA 1 cut(s) 684
Hpy8I GTNNAC 1 cut(s) 554
HpyAV CCTTC 2 cut(s) 392, 516
HpyCH4III ACNGT 2 cut(s) 30, 79
HpyCH4IV ACGT 1 cut(s) 550
HpyCH4V TGCA 7 cut(s) 185, 189, 437, 477, 536, 559, 653
HpyF10VI GCNNNNNNNGC 7 cut(s) 186, 422, 428, 431, 434, 437, 440
HpyF3I CTNAG 5 cut(s) 485, 506, 578, 682, 691
HpySE526I ACGT 1 cut(s) 550
Hsp92II CATG 1 cut(s) 189
Kzo9I GATC 1 cut(s) 571
LpnPI CCDG 7 cut(s) 400, 457, 479, 616, 649, 669, 676
Lsp1109I GCAGC 5 cut(s) 412, 421, 449, 520, 548
LweI GCATC 1 cut(s) 589
MaeI CTAG 3 cut(s) 320, 540, 730
MaeII ACGT 1 cut(s) 550
MalI GATC 1 cut(s) 573
MboI GATC 1 cut(s) 571
MlsI TGGCCA 1 cut(s) 674
MluCI AATT 7 cut(s) 17, 50, 101, 117, 173, 202, 304
MluNI TGGCCA 1 cut(s) 674
MmeI TCCRAC 1 cut(s) 603
MnlI CCTC 5 cut(s) 70, 122, 386, 670, 691
Mox20I TGGCCA 1 cut(s) 674
MscI TGGCCA 1 cut(s) 674
MseI TTAA 5 cut(s) 6, 201, 233, 240, 591
MslI CAYNNNNRTG 1 cut(s) 559
Msp20I TGGCCA 1 cut(s) 674
MspA1I CMGCKG 2 cut(s) 425, 434
MspI CCGG 1 cut(s) 387
MspR9I CCNGG 1 cut(s) 664
MvaI CCWGG 1 cut(s) 664
MwoI GCNNNNNNNGC 7 cut(s) 186, 422, 428, 431, 434, 437, 440
NdeII GATC 1 cut(s) 571
NlaIII CATG 1 cut(s) 189
NlaIV GGNNCC 2 cut(s) 110, 406
NspI RCATGY 1 cut(s) 189
OliI CACNNNNGTG 1 cut(s) 559
PaeI GCATGC 1 cut(s) 189
PinAI ACCGGT 1 cut(s) 386
PkrI GCNGC 9 cut(s) 424, 427, 430, 433, 436, 439, 442, 535, 538
Psp1406I AACGTT 1 cut(s) 550
Psp6I CCWGG 1 cut(s) 662
PspGI CCWGG 1 cut(s) 662
PspN4I GGNNCC 2 cut(s) 110, 406
PspPI GGNCC 1 cut(s) 360
PsrI GAACNNNNNNTAC 2 cut(s) 535, 567
PstI CTGCAG 3 cut(s) 439, 538, 561
RsaI GTAC 1 cut(s) 155
RsaNI GTAC 1 cut(s) 154
RseI CAYNNNNRTG 1 cut(s) 559
SaqAI TTAA 5 cut(s) 6, 201, 233, 240, 591
SatI GCNGC 9 cut(s) 423, 426, 429, 432, 435, 438, 441, 534, 537
Sau3AI GATC 1 cut(s) 571
Sau96I GGNCC 1 cut(s) 360
ScrFI CCNGG 1 cut(s) 664
SetI ASST 8 cut(s) 114, 403, 507, 541, 553, 584, 668, 697
SfaNI GCATC 1 cut(s) 589
SfcI CTRYAG 3 cut(s) 435, 534, 557
SmiMI CAYNNNNRTG 1 cut(s) 559
SphI GCATGC 1 cut(s) 189
Sse9I AATT 7 cut(s) 17, 50, 101, 117, 173, 202, 304
SsiI CCGC 5 cut(s) 420, 423, 429, 432, 441
SspMI CTAG 3 cut(s) 320, 540, 730
StyD4I CCNGG 1 cut(s) 662
StyI CCWWGG 1 cut(s) 704
TaaI ACNGT 2 cut(s) 30, 79
TaiI ACGT 1 cut(s) 553
TasI AATT 7 cut(s) 17, 50, 101, 117, 173, 202, 304
TauI GCSGC 4 cut(s) 425, 431, 434, 443
Tru1I TTAA 5 cut(s) 6, 201, 233, 240, 591
Tru9I TTAA 5 cut(s) 6, 201, 233, 240, 591
TscAI CASTG 5 cut(s) 479, 561, 566, 582, 610
TseI GCWGC 5 cut(s) 425, 434, 437, 533, 536
TspDTI ATGAA 2 cut(s) 101, 453
TspRI CASTG 5 cut(s) 479, 561, 566, 582, 610
XapI RAATTY 4 cut(s) 101, 117, 173, 304
XceI RCATGY 1 cut(s) 189
XspI CTAG 3 cut(s) 320, 540, 730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.