MD05G1283100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0016522
Physical Location & Seq
Forward (+)
41662061 .. 41662345
285 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1283100.v1.1.491

Sequence Viewer

Length: 285 bp
ATGGGGGCAATAACGACCTCAGTGATTGCGATAGTGGGAGTGGTTCTCGGTTGGATCGCCATCGAGATTGCCTGCAAGCCCTGCCTCGAACAGGGCCGCGAAGCCATCGATCGCAACCTCAATCCCGACTACGATCCAGACGACGCCGTCGTCTCCTCCGACATCCGCGCCCCTCTCAACCCTCCCGCCGTCAATACCATCGCAGTGGATCTCGAGGCGGAGGATCCCAAGGCCGCTGCGGCCATCGCAGCTTCTTCACATTCCGATGGCGTCAAGGCCGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

95

Amino Acids

9.65

Weight (kDa)

4.21

Isoelectric Point (pI)

32.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016522)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34585
fragaria_vesca FvH4_3g05470
malus_domestica MD05G1283100.v1.1 MD10G1261400.v1.1
prunus_persica Prupe.4G081500_v2.0.a1
pyrus_communis pycom10g21760
rosa_chinensis RchiOBHm_Chr5g0014281
rosa_multiflora Rmu_sc0009295.1_g000010
rosa_roxburghii Rroxscaffold_1G00062290
rosa_rugosa Rorug05G0016800
rosa_samantha Rh5BG108400 Rh5CG120000 Rh5DG107000
rosa_wichuraiana Rw5G009700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 146, 149
AccII CGCG 2 cut(s) 99, 168
AciI CCGC 6 cut(s) 97, 166, 186, 218, 234, 239
AclWI GGATC 5 cut(s) 62, 128, 216, 218, 231
AcoI YGGCCR 1 cut(s) 240
AcyI GRCGYC 2 cut(s) 144, 270
AfiI CCNNNNNNNGG 1 cut(s) 91
AluBI AGCT 1 cut(s) 251
AluI AGCT 1 cut(s) 251
Alw26I GTCTC 1 cut(s) 157
AlwI GGATC 5 cut(s) 62, 128, 216, 218, 231
Ama87I CYCGRG 1 cut(s) 212
AoxI GGCC 4 cut(s) 94, 231, 240, 276
ApeKI GCWGC 2 cut(s) 236, 248
AspLEI GCGC 1 cut(s) 170
AspS9I GGNCC 1 cut(s) 94
AvaI CYCGRG 1 cut(s) 212
BamHI GGATCC 1 cut(s) 223
BbvI GCAGC 2 cut(s) 223, 260
BccI CCATC 5 cut(s) 68, 113, 206, 251, 260
BceAI ACGGC 3 cut(s) 131, 173, 263
BcoDI GTCTC 1 cut(s) 157
BglI GCCNNNNNGGC 1 cut(s) 239
BisI GCNGC 5 cut(s) 97, 234, 237, 240, 249
BlsI GCNGC 5 cut(s) 98, 235, 238, 241, 250
BmeT110I CYCGRG 1 cut(s) 212
BmgT120I GGNCC 1 cut(s) 94
BmiI GGNNCC 1 cut(s) 225
BplI GAGNNNNNCTC 2 cut(s) 30, 62
Bsa29I ATCGAT 1 cut(s) 108
BsaBI GATNNNNATC 1 cut(s) 59
BsaHI GRCGYC 2 cut(s) 144, 270
BsaJI CCNNGG 1 cut(s) 228
Bsc4I CCNNNNNNNGG 1 cut(s) 91
Bse8I GATNNNNATC 1 cut(s) 59
BseCI ATCGAT 1 cut(s) 108
BseDI CCNNGG 1 cut(s) 228
BseGI GGATG 1 cut(s) 162
BseJI GATNNNNATC 1 cut(s) 59
BseLI CCNNNNNNNGG 1 cut(s) 91
BseMII CTCAG 1 cut(s) 33
BseRI GAGGAG 1 cut(s) 145
BseXI GCAGC 2 cut(s) 223, 260
Bsh1236I CGCG 2 cut(s) 99, 168
Bsh1285I CGRYCG 1 cut(s) 112
BshFI GGCC 4 cut(s) 96, 233, 242, 278
BshVI ATCGAT 1 cut(s) 108
BsiEI CGRYCG 1 cut(s) 112
BsiHKCI CYCGRG 1 cut(s) 212
BslI CCNNNNNNNGG 1 cut(s) 91
BsmAI GTCTC 1 cut(s) 157
BsmBI CGTCTC 1 cut(s) 157
BsnI GGCC 4 cut(s) 96, 233, 242, 278
BsoBI CYCGRG 1 cut(s) 212
Bsp143I GATC 5 cut(s) 54, 109, 133, 208, 223
BspACI CCGC 6 cut(s) 97, 166, 186, 218, 234, 239
BspANI GGCC 4 cut(s) 96, 233, 242, 278
BspCNI CTCAG 1 cut(s) 32
BspDI ATCGAT 1 cut(s) 108
BspFNI CGCG 2 cut(s) 99, 168
BspLI GGNNCC 1 cut(s) 225
BspPI GGATC 5 cut(s) 62, 128, 216, 218, 231
BssECI CCNNGG 1 cut(s) 228
BssMI GATC 5 cut(s) 54, 109, 133, 208, 223
BssNI GRCGYC 2 cut(s) 144, 270
BssT1I CCWWGG 1 cut(s) 228
BstACI GRCGYC 2 cut(s) 144, 270
BstAPI GCANNNNNTGC 1 cut(s) 81
BstC8I GCNNGC 2 cut(s) 73, 77
BstDEI CTNAG 1 cut(s) 19
BstENI CCTNNNNNAGG 1 cut(s) 89
BstF5I GGATG 1 cut(s) 162
BstFNI CGCG 2 cut(s) 99, 168
BstHHI GCGC 1 cut(s) 170
BstKTI GATC 5 cut(s) 57, 112, 136, 211, 226
BstMAI GTCTC 1 cut(s) 157
BstMBI GATC 5 cut(s) 54, 109, 133, 208, 223
BstMCI CGRYCG 1 cut(s) 112
BstMWI GCNNNNNNNGC 4 cut(s) 81, 239, 245, 248
BstUI CGCG 2 cut(s) 99, 168
BstV1I GCAGC 2 cut(s) 223, 260
BstX2I RGATCY 2 cut(s) 208, 223
BstXI CCANNNNNNTGG 1 cut(s) 205
BstYI RGATCY 2 cut(s) 208, 223
Bsu15I ATCGAT 1 cut(s) 108
BsuRI GGCC 4 cut(s) 96, 233, 242, 278
BsuTUI ATCGAT 1 cut(s) 108
BtgZI GCGATG 2 cut(s) 184, 229
BtsCI GGATG 1 cut(s) 162
BtsI GCAGTG 1 cut(s) 210
BtsIMutI CAGTG 2 cut(s) 27, 210
Cac8I GCNNGC 2 cut(s) 73, 77
CfoI GCGC 1 cut(s) 170
Cfr13I GGNCC 1 cut(s) 94
ClaI ATCGAT 1 cut(s) 108
CseI GACGC 2 cut(s) 152, 259
CviJI RGCY 7 cut(s) 79, 96, 104, 233, 242, 251, 278
CviKI_1 RGCY 7 cut(s) 79, 96, 104, 233, 242, 251, 278
DdeI CTNAG 1 cut(s) 19
DpnI GATC 5 cut(s) 56, 111, 135, 210, 225
DpnII GATC 5 cut(s) 54, 109, 133, 208, 223
DrdI GACNNNNNNGTC 2 cut(s) 146, 149
DseDI GACNNNNNNGTC 2 cut(s) 146, 149
EaeI YGGCCR 1 cut(s) 240
EciI GGCGGA 1 cut(s) 233
Eco130I CCWWGG 1 cut(s) 228
Eco88I CYCGRG 1 cut(s) 212
EcoNI CCTNNNNNAGG 1 cut(s) 89
EcoT14I CCWWGG 1 cut(s) 228
ErhI CCWWGG 1 cut(s) 228
Esp3I CGTCTC 1 cut(s) 157
FauI CCCGC 1 cut(s) 193
Fnu4HI GCNGC 5 cut(s) 97, 234, 237, 240, 249
FokI GGATG 1 cut(s) 149
Fsp4HI GCNGC 5 cut(s) 97, 234, 237, 240, 249
GlaI GCGC 1 cut(s) 169
GluI GCNGC 5 cut(s) 97, 234, 237, 240, 249
HaeIII GGCC 4 cut(s) 96, 233, 242, 278
HgaI GACGC 2 cut(s) 152, 259
HhaI GCGC 1 cut(s) 170
Hin1I GRCGYC 2 cut(s) 144, 270
Hin6I GCGC 1 cut(s) 168
HinP1I GCGC 1 cut(s) 168
Hpy188I TCNGA 3 cut(s) 160, 265, 284
Hpy188III TCNNGA 4 cut(s) 64, 125, 137, 212
Hpy99I CGWCG 2 cut(s) 146, 152
HpyCH4V TGCA 1 cut(s) 75
HpyF10VI GCNNNNNNNGC 4 cut(s) 81, 239, 245, 248
HpyF3I CTNAG 1 cut(s) 19
Hsp92I GRCGYC 2 cut(s) 144, 270
HspAI GCGC 1 cut(s) 168
Kzo9I GATC 5 cut(s) 54, 109, 133, 208, 223
LpnPI CCDG 4 cut(s) 77, 85, 94, 150
Lsp1109I GCAGC 2 cut(s) 223, 260
MalI GATC 5 cut(s) 56, 111, 135, 210, 225
MboI GATC 5 cut(s) 54, 109, 133, 208, 223
MboII GAAGA 1 cut(s) 246
MflI RGATCY 2 cut(s) 208, 223
MmeI TCCRAC 2 cut(s) 32, 183
MnlI CCTC 8 cut(s) 28, 95, 128, 166, 183, 192, 208, 214
MslI CAYNNNNRTG 2 cut(s) 203, 264
MspA1I CMGCKG 1 cut(s) 236
MvnI CGCG 2 cut(s) 99, 168
MwoI GCNNNNNNNGC 4 cut(s) 81, 239, 245, 248
NdeII GATC 5 cut(s) 54, 109, 133, 208, 223
NlaIV GGNNCC 1 cut(s) 225
PaeR7I CTCGAG 1 cut(s) 212
PcsI WCGNNNNNNNCGW 3 cut(s) 138, 147, 156
PflFI GACNNNGTC 1 cut(s) 146
PkrI GCNGC 5 cut(s) 98, 235, 238, 241, 250
Ple19I CGATCG 1 cut(s) 112
PspN4I GGNNCC 1 cut(s) 225
PspPI GGNCC 1 cut(s) 94
PsuI RGATCY 2 cut(s) 208, 223
PsyI GACNNNGTC 1 cut(s) 146
PvuI CGATCG 1 cut(s) 112
RseI CAYNNNNRTG 2 cut(s) 203, 264
SatI GCNGC 5 cut(s) 97, 234, 237, 240, 249
Sau3AI GATC 5 cut(s) 54, 109, 133, 208, 223
Sau96I GGNCC 1 cut(s) 94
SetI ASST 3 cut(s) 20, 120, 253
SfiI GGCCNNNNNGGCC 1 cut(s) 239
Sfr274I CTCGAG 1 cut(s) 212
SlaI CTCGAG 1 cut(s) 212
SmiMI CAYNNNNRTG 2 cut(s) 203, 264
SmlI CTYRAG 1 cut(s) 212
SmoI CTYRAG 1 cut(s) 212
SsiI CCGC 6 cut(s) 97, 166, 186, 218, 234, 239
StyI CCWWGG 1 cut(s) 228
TaqI TCGA 4 cut(s) 63, 87, 108, 213
TauI GCSGC 3 cut(s) 99, 236, 242
TscAI CASTG 2 cut(s) 27, 210
TseI GCWGC 2 cut(s) 236, 248
TspRI CASTG 2 cut(s) 27, 210
Tth111I GACNNNGTC 1 cut(s) 146
XagI CCTNNNNNAGG 1 cut(s) 89
XhoI CTCGAG 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.