MD10G1261400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0016522
Physical Location & Seq
Forward (+)
35564956 .. 35565225
270 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1261400.v1.1.491

Sequence Viewer

Length: 270 bp
ATGGGGGCAATAACGGCCTCAGTGATTGCGATAGTGGGAGTGGTTCTCGGTTGGATCGCCATCGAGATTGCCTGCAAACCTTGCCTCGAACAGGGCCGCCAAGCCATCGATCGCAGTCTCAATCCCGACTACGATCCCGACGACGACGTCAGCGCTCCTCTCAACCCTCCCGCCGTCAACACCACCGCCGTGGTTCTCGAGGCGGAGGATCCCAAAAGCGCCGCTGCCATCGCAGCTTCTTCTCATTCCGATGCCATCAAGGCCGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

90

Amino Acids

9.08

Weight (kDa)

4.21

Isoelectric Point (pI)

38.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016522)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34585
fragaria_vesca FvH4_3g05470
malus_domestica MD05G1283100.v1.1 MD10G1261400.v1.1
prunus_persica Prupe.4G081500_v2.0.a1
pyrus_communis pycom10g21760
rosa_chinensis RchiOBHm_Chr5g0014281
rosa_multiflora Rmu_sc0009295.1_g000010
rosa_roxburghii Rroxscaffold_1G00062290
rosa_rugosa Rorug05G0016800
rosa_samantha Rh5BG108400 Rh5CG120000 Rh5DG107000
rosa_wichuraiana Rw5G009700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 146
AatII GACGTC 1 cut(s) 150
AciI CCGC 5 cut(s) 97, 171, 186, 203, 222
AclWI GGATC 4 cut(s) 62, 128, 203, 216
AcyI GRCGYC 1 cut(s) 147
AfeI AGCGCT 1 cut(s) 154
AfiI CCNNNNNNNGG 1 cut(s) 91
AleI CACNNNNGTG 1 cut(s) 188
AluBI AGCT 1 cut(s) 236
AluI AGCT 1 cut(s) 236
Alw26I GTCTC 1 cut(s) 122
AlwI GGATC 4 cut(s) 62, 128, 203, 216
Ama87I CYCGRG 1 cut(s) 197
Aor51HI AGCGCT 1 cut(s) 154
AoxI GGCC 3 cut(s) 15, 94, 261
ApeKI GCWGC 2 cut(s) 224, 233
AspLEI GCGC 2 cut(s) 155, 221
AspS9I GGNCC 1 cut(s) 94
AvaI CYCGRG 1 cut(s) 197
BamHI GGATCC 1 cut(s) 208
BbvI GCAGC 2 cut(s) 211, 245
BccI CCATC 4 cut(s) 68, 113, 236, 263
BceAI ACGGC 4 cut(s) 30, 158, 173, 248
BcoDI GTCTC 1 cut(s) 122
BfoI RGCGCY 2 cut(s) 156, 222
BglI GCCNNNNNGGC 1 cut(s) 260
BisI GCNGC 4 cut(s) 97, 222, 225, 234
BlsI GCNGC 4 cut(s) 98, 223, 226, 235
BmeT110I CYCGRG 1 cut(s) 197
BmgT120I GGNCC 1 cut(s) 94
BmiI GGNNCC 1 cut(s) 210
BmsI GCATC 1 cut(s) 241
BplI GAGNNNNNCTC 2 cut(s) 30, 62
Bsa29I ATCGAT 1 cut(s) 108
BsaBI GATNNNNATC 1 cut(s) 59
BsaHI GRCGYC 1 cut(s) 147
BsaJI CCNNGG 1 cut(s) 189
Bsc4I CCNNNNNNNGG 1 cut(s) 91
Bse8I GATNNNNATC 1 cut(s) 59
BseCI ATCGAT 1 cut(s) 108
BseDI CCNNGG 1 cut(s) 189
BseJI GATNNNNATC 1 cut(s) 59
BseLI CCNNNNNNNGG 1 cut(s) 91
BseMII CTCAG 1 cut(s) 33
BseRI GAGGAG 1 cut(s) 147
BseXI GCAGC 2 cut(s) 211, 245
Bsh1285I CGRYCG 1 cut(s) 112
BshFI GGCC 3 cut(s) 17, 96, 263
BshVI ATCGAT 1 cut(s) 108
BsiEI CGRYCG 1 cut(s) 112
BsiHKCI CYCGRG 1 cut(s) 197
BslI CCNNNNNNNGG 1 cut(s) 91
BsmAI GTCTC 1 cut(s) 122
BsnI GGCC 3 cut(s) 17, 96, 263
BsoBI CYCGRG 1 cut(s) 197
Bsp143I GATC 4 cut(s) 54, 109, 133, 208
BspACI CCGC 5 cut(s) 97, 171, 186, 203, 222
BspANI GGCC 3 cut(s) 17, 96, 263
BspCNI CTCAG 1 cut(s) 32
BspDI ATCGAT 1 cut(s) 108
BspLI GGNNCC 1 cut(s) 210
BspPI GGATC 4 cut(s) 62, 128, 203, 216
BssECI CCNNGG 1 cut(s) 189
BssMI GATC 4 cut(s) 54, 109, 133, 208
BssNI GRCGYC 1 cut(s) 147
BstACI GRCGYC 1 cut(s) 147
BstAPI GCANNNNNTGC 1 cut(s) 81
BstC8I GCNNGC 1 cut(s) 73
BstDEI CTNAG 1 cut(s) 19
BstDSI CCRYGG 1 cut(s) 189
BstENI CCTNNNNNAGG 1 cut(s) 89
BstH2I RGCGCY 2 cut(s) 156, 222
BstHHI GCGC 2 cut(s) 155, 221
BstKTI GATC 4 cut(s) 57, 112, 136, 211
BstMAI GTCTC 1 cut(s) 122
BstMBI GATC 4 cut(s) 54, 109, 133, 208
BstMCI CGRYCG 1 cut(s) 112
BstMWI GCNNNNNNNGC 5 cut(s) 14, 81, 230, 233, 260
BstV1I GCAGC 2 cut(s) 211, 245
BstX2I RGATCY 1 cut(s) 208
BstXI CCANNNNNNTGG 1 cut(s) 190
BstYI RGATCY 1 cut(s) 208
Bsu15I ATCGAT 1 cut(s) 108
BsuRI GGCC 3 cut(s) 17, 96, 263
BsuTUI ATCGAT 1 cut(s) 108
BtgI CCRYGG 1 cut(s) 189
BtgZI GCGATG 1 cut(s) 214
BtsIMutI CAGTG 1 cut(s) 27
Cac8I GCNNGC 1 cut(s) 73
CfoI GCGC 2 cut(s) 155, 221
Cfr13I GGNCC 1 cut(s) 94
ClaI ATCGAT 1 cut(s) 108
CviJI RGCY 5 cut(s) 17, 96, 104, 236, 263
CviKI_1 RGCY 5 cut(s) 17, 96, 104, 236, 263
DdeI CTNAG 1 cut(s) 19
DpnI GATC 4 cut(s) 56, 111, 135, 210
DpnII GATC 4 cut(s) 54, 109, 133, 208
DrdI GACNNNNNNGTC 1 cut(s) 146
DseDI GACNNNNNNGTC 1 cut(s) 146
EciI GGCGGA 1 cut(s) 218
Eco47III AGCGCT 1 cut(s) 154
Eco88I CYCGRG 1 cut(s) 197
EcoNI CCTNNNNNAGG 1 cut(s) 89
FauI CCCGC 1 cut(s) 178
Fnu4HI GCNGC 4 cut(s) 97, 222, 225, 234
Fsp4HI GCNGC 4 cut(s) 97, 222, 225, 234
GlaI GCGC 2 cut(s) 154, 220
GluI GCNGC 4 cut(s) 97, 222, 225, 234
HaeII RGCGCY 2 cut(s) 156, 222
HaeIII GGCC 3 cut(s) 17, 96, 263
HhaI GCGC 2 cut(s) 155, 221
Hin1I GRCGYC 1 cut(s) 147
Hin6I GCGC 2 cut(s) 153, 219
HinP1I GCGC 2 cut(s) 153, 219
HincII GTYRAC 1 cut(s) 178
HindII GTYRAC 1 cut(s) 178
Hpy166II GTNNAC 1 cut(s) 178
Hpy188I TCNGA 2 cut(s) 250, 269
Hpy188III TCNNGA 4 cut(s) 64, 125, 137, 197
Hpy8I GTNNAC 1 cut(s) 178
Hpy99I CGWCG 3 cut(s) 143, 146, 149
HpyCH4IV ACGT 1 cut(s) 147
HpyCH4V TGCA 1 cut(s) 75
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 81, 230, 233, 260
HpyF3I CTNAG 1 cut(s) 19
HpySE526I ACGT 1 cut(s) 147
Hsp92I GRCGYC 1 cut(s) 147
HspAI GCGC 2 cut(s) 153, 219
Kzo9I GATC 4 cut(s) 54, 109, 133, 208
LmnI GCTCC 1 cut(s) 160
LpnPI CCDG 2 cut(s) 77, 85
Lsp1109I GCAGC 2 cut(s) 211, 245
LweI GCATC 1 cut(s) 241
MaeII ACGT 1 cut(s) 147
MalI GATC 4 cut(s) 56, 111, 135, 210
MboI GATC 4 cut(s) 54, 109, 133, 208
MboII GAAGA 1 cut(s) 231
MflI RGATCY 1 cut(s) 208
MmeI TCCRAC 1 cut(s) 32
MnlI CCTC 6 cut(s) 28, 95, 168, 177, 193, 199
MslI CAYNNNNRTG 2 cut(s) 188, 249
MspA1I CMGCKG 1 cut(s) 224
MwoI GCNNNNNNNGC 5 cut(s) 14, 81, 230, 233, 260
NdeII GATC 4 cut(s) 54, 109, 133, 208
NlaIV GGNNCC 1 cut(s) 210
OliI CACNNNNGTG 1 cut(s) 188
PaeR7I CTCGAG 1 cut(s) 197
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PflFI GACNNNGTC 1 cut(s) 146
PkrI GCNGC 4 cut(s) 98, 223, 226, 235
Ple19I CGATCG 1 cut(s) 112
PspN4I GGNNCC 1 cut(s) 210
PspPI GGNCC 1 cut(s) 94
PsuI RGATCY 1 cut(s) 208
PsyI GACNNNGTC 1 cut(s) 146
PvuI CGATCG 1 cut(s) 112
RseI CAYNNNNRTG 2 cut(s) 188, 249
SatI GCNGC 4 cut(s) 97, 222, 225, 234
Sau3AI GATC 4 cut(s) 54, 109, 133, 208
Sau96I GGNCC 1 cut(s) 94
SetI ASST 3 cut(s) 82, 150, 238
SfaNI GCATC 1 cut(s) 241
Sfr274I CTCGAG 1 cut(s) 197
SlaI CTCGAG 1 cut(s) 197
SmiMI CAYNNNNRTG 2 cut(s) 188, 249
SmlI CTYRAG 1 cut(s) 197
SmoI CTYRAG 1 cut(s) 197
SsiI CCGC 5 cut(s) 97, 171, 186, 203, 222
TaiI ACGT 1 cut(s) 150
TaqI TCGA 4 cut(s) 63, 87, 108, 198
TauI GCSGC 2 cut(s) 99, 224
TscAI CASTG 1 cut(s) 27
TseI GCWGC 2 cut(s) 224, 233
TspRI CASTG 1 cut(s) 27
Tth111I GACNNNGTC 1 cut(s) 146
XagI CCTNNNNNAGG 1 cut(s) 89
XhoI CTCGAG 1 cut(s) 197
ZraI GACGTC 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.