Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0011307

Physical Location & Seq

Genomic Coordinates Forward (+)

591522 .. 594800

Gene Structure 3279 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1005300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1602 bp

ATGGGCACAGCTCGTAGTTTGACGGAGGAAGAGAGAAAGAGGGGCAGAGAAATTACTTGGGATGATAAGGAAGTCTGCGGGCACTATTTGGTTCGGTTTTGTCCGTTCCATCTCTTCGCCAATACTCGAAGCGATCTAGGACAGTGCCCTAAAATCCATGACCCGAAGTTGAAGGAAAGTTTTGAGAAGTCACCACAGCATGATGCATACGTGCCCAAGTTTGAAGCCGAACTTGCTCAGTTTTGTGAGATGTTGGTGATGGATTTGGATAGAAGAGTAAGACGTGAGCGAGAACACCGTCACCAAGAGGTGGAACCTGCACTACCACATCCACTTTCAATTGAAAAGTCCAAACATTTGTTTATTTTGGAGGATAAGTTAAAGAAGTTGCTTGAACAAGTGGAGGCCCTTGGTGAATCCAGCAAGATGGATGAAGCTGAAGTGTCCGTGAGAGAGATGTCCAATAAAATTGTTGTGGAGAATCCTCAAGTTGGTCAGGGCCGTCAGGCTCAGGGTGGTTCAAGTGCCCCTACTCTTTTTGGTAATATCCAAACAAACTGGTCCAGTGTTATACTCCAGCAGATCTCAGTCTACGGTATAGCTGCTGGTTACTGCTTATCTGCCTCATTGCTTTCCATTATCAACAAGTGGGCTGTCGTGAAATTTCCTTATCCCGGGGCCCTAACTGCTTTACAGTATTTTACAAGTGCTATGGGGGTCCTTGTATGTGGCTGGCTTAAGTTGCTAGAACATGATTCGCTTGACCTTCTTACTATGTGGCGCTTCCTACCAGCAGCAGTTATATTCTACCTCTCTCTCTTCACCAACAGTGAGCTCCTTCTTCATGCCAATGTCGACACTTTCATTGTCTTTCGTTCAGTTGTTCCAATCTTTGTTGCTATAGGAGAGACTCTCTTCTTACACCAGCCATGGCCATCAATTAGGACATGGGCCTCACTTGCCACAATTTTTGGGGGAAGTGTGATTTATGTGCTTACAGATTACCAGTTCACAGTCACGGCTTATAGTTGGGCTCTAGCTTACTTGGTAAGCATGTCCATAGATTTTGTCTACATAAAACATGTGGTAATGACCATCGGCTTAAACACATGGGGTCTTGTATTGTATAACAATCTCGAGGCTCTCCTACTGTTCCCGCTGGAACTACTTGTAATGGGTGAATTGAAGAAGATTAAGCATGAAATCACGGACGAATCAGATTGGTATTCTTTTGATGTGCTTTTGCCAGTGGGGTTATCATGTTTATTTGGTTTGTCTATCTCTTTCTTTGGGTTTTCTTGCCGAAGGGCAATTTCTGCAACTGGATTTACTGTTCTTGGTATAGTGAACAAGCTATTGACTGTTGTGATTAATCTGATTATTTGGGACAAGCATTCAACCATAGTTGGCACAGTGGGGCTGTTAATATGTATGCTAGGCGGTGTTATGTATCAGCAATCTACAAGCAACAAGCCTAAGGTTGTAAATCAGGTGAAAGCACAAGAGGCGGAGGAAGAACAACAAAAGTTAATTGAGATGAACTCCAACTTAGAAAGCAATAGCAATGAGAAATGGAATGCTGAATCAGAACAGGGAAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0006810 GO:0008150 GO:0015780 GO:0015931 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:1901264

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TPT

Protein Analysis

534

Amino Acids

60.16

Weight (kDa)

5.75

Isoelectric Point (pI)

38.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LUC7	PF03194	1 - 154	9e-39	LUC7 N_terminus
TPT	PF03151	208 - 484	3.3e-16	Triose-phosphate Transporter family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011307)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G76340
fragaria_vesca	FvH4_5g25930 FvH4_5g25930 FvH4_5g25930
malus_domestica	MD06G1005300.v1.1
prunus_persica	Prupe.3G152800_v2.0.a1 Prupe.5G006600_v2.0.a1 Prupe.5G006600_v2.0.a1 Prupe.5G006600_v2.0.a1
pyrus_communis	pycom06g00500 pycom16g26160
rosa_chinensis	RchiOBHm_Chr7g0216461
rosa_laevigata	RLG00000002569
rosa_multiflora	Rmu_ssc0000454.1_g000027
rosa_roxburghii	Rroxscaffold_3G00243220
rosa_rugosa	Rorug07G0160400
rosa_samantha	Rh7AG299600 Rh7BG291100 Rh7DG300900
rosa_wichuraiana	Rw7G025460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	325
AccB7I	CCANNNNNTGG	1 cut(s)	310
AccI	GTMKAC	3 cut(s)	591, 855, 1071
AciI	CCGC	4 cut(s)	78, 1157, 1440, 1508
AcoI	YGGCCR	1 cut(s)	932
AcsI	RAATTY	1 cut(s)	662
AcuI	CTGAAG	1 cut(s)	459
AfiI	CCNNNNNNNGG	3 cut(s)	310, 491, 674
AflII	CTTAAG	1 cut(s)	737
AflIII	ACRYGT	1 cut(s)	1081
AgsI	TTSAA	8 cut(s)	172, 224, 339, 344, 395, 522, 1186, 1398
AjiI	CACGTC	1 cut(s)	284
AloI	GAACNNNNNNTCC	2 cut(s)	1317, 1349
AluBI	AGCT	6 cut(s)	11, 437, 602, 835, 1040, 1354
AluI	AGCT	6 cut(s)	11, 437, 602, 835, 1040, 1354
Alw21I	GWGCWC	1 cut(s)	837
Alw26I	GTCTC	1 cut(s)	902
Ama87I	CYCGRG	2 cut(s)	674, 1136
AoxI	GGCC	5 cut(s)	405, 499, 678, 932, 951
ApaI	GGGCCC	1 cut(s)	682
ApeKI	GCWGC	2 cut(s)	602, 794
ApoI	RAATTY	1 cut(s)	662
AseI	ATTAAT	1 cut(s)	1371
AspLEI	GCGC	1 cut(s)	783
AspS9I	GGNCC	7 cut(s)	406, 499, 561, 678, 679, 718, 951
AsuC2I	CCSGG	2 cut(s)	675, 676
AsuHPI	GGTGA	7 cut(s)	183, 268, 293, 425, 814, 1190, 1504
AvaI	CYCGRG	2 cut(s)	674, 1136
AvaII	GGWCC	2 cut(s)	561, 718
AxyI	CCTNAGG	1 cut(s)	1476
BaeGI	GKGCMC	6 cut(s)	8, 84, 149, 216, 529, 682
BalI	TGGCCA	1 cut(s)	934
BanII	GRGCYC	3 cut(s)	682, 837, 1036
Bbv12I	GWGCWC	1 cut(s)	837
BbvI	GCAGC	2 cut(s)	589, 806
BccI	CCATC	5 cut(s)	117, 253, 421, 943, 1103
BceAI	ACGGC	2 cut(s)	486, 1035
BcnI	CCSGG	2 cut(s)	675, 676
BcoDI	GTCTC	1 cut(s)	902
BfaI	CTAG	4 cut(s)	137, 746, 1037, 1436
BfmI	CTRYAG	1 cut(s)	900
BfoI	RGCGCY	1 cut(s)	784
BfrI	CTTAAG	1 cut(s)	737
BfuAI	ACCTGC	1 cut(s)	325
BglII	AGATCT	1 cut(s)	582
BisI	GCNGC	2 cut(s)	603, 795
BlsI	GCNGC	2 cut(s)	604, 796
Bme1390I	CCNGG	2 cut(s)	675, 676
Bme18I	GGWCC	2 cut(s)	561, 718
BmeT110I	CYCGRG	2 cut(s)	674, 1136
BmgBI	CACGTC	1 cut(s)	284
BmgT120I	GGNCC	7 cut(s)	406, 499, 561, 678, 679, 718, 951
BmiI	GGNNCC	4 cut(s)	315, 679, 680, 719
BmrFI	CCNGG	2 cut(s)	675, 676
BmsI	GCATC	1 cut(s)	193
BplI	GAGNNNNNCTC	4 cut(s)	897, 929, 1526, 1558
BpmI	CTGGAG	1 cut(s)	560
Bpu10I	CCTNAGC	1 cut(s)	510
BpuEI	CTTGAG	1 cut(s)	471
BpuMI	CCSGG	2 cut(s)	675, 676
BsaAI	YACGTR	1 cut(s)	211
BsaJI	CCNNGG	4 cut(s)	409, 674, 675, 929
BsaXI	ACNNNNNCTCC	2 cut(s)	362, 392
Bsc4I	CCNNNNNNNGG	3 cut(s)	310, 491, 674
Bse1I	ACTGG	5 cut(s)	563, 564, 1006, 1247, 1327
Bse21I	CCTNAGG	1 cut(s)	1476
Bse3DI	GCAATG	2 cut(s)	626, 1570
BseDI	CCNNGG	4 cut(s)	409, 674, 675, 929
BseGI	GGATG	3 cut(s)	67, 328, 436
BseLI	CCNNNNNNNGG	3 cut(s)	310, 491, 674
BseMI	GCAATG	2 cut(s)	626, 1570
BseMII	CTCAG	3 cut(s)	251, 524, 600
BseNI	ACTGG	5 cut(s)	563, 564, 1006, 1247, 1327
BseSI	GKGCMC	6 cut(s)	8, 84, 149, 216, 529, 682
BseXI	GCAGC	2 cut(s)	589, 806
BsgI	GTGCAG	1 cut(s)	303
BshFI	GGCC	5 cut(s)	407, 501, 680, 934, 953
BsiHKAI	GWGCWC	1 cut(s)	837
BsiHKCI	CYCGRG	2 cut(s)	674, 1136
BsiSI	CCGG	1 cut(s)	675
BslFI	GGGAC	1 cut(s)	1400
BslI	CCNNNNNNNGG	3 cut(s)	310, 491, 674
BsmAI	GTCTC	1 cut(s)	902
BsmFI	GGGAC	1 cut(s)	1400
BsmI	GAATGC	2 cut(s)	1393, 1582
BsnI	GGCC	5 cut(s)	407, 501, 680, 934, 953
BsoBI	CYCGRG	2 cut(s)	674, 1136
Bsp120I	GGGCCC	1 cut(s)	678
Bsp1286I	GDGCHC	8 cut(s)	8, 84, 149, 216, 529, 682, 837, 1036
Bsp143I	GATC	2 cut(s)	133, 582
Bsp19I	CCATGG	1 cut(s)	929
BspACI	CCGC	4 cut(s)	78, 1157, 1440, 1508
BspANI	GGCC	5 cut(s)	407, 501, 680, 934, 953
BspCNI	CTCAG	3 cut(s)	250, 523, 599
BspLI	GGNNCC	4 cut(s)	315, 679, 680, 719
BspMI	ACCTGC	1 cut(s)	325
BspTI	CTTAAG	1 cut(s)	737
BsrDI	GCAATG	2 cut(s)	626, 1570
BsrI	ACTGG	5 cut(s)	563, 564, 1006, 1247, 1327
BssECI	CCNNGG	4 cut(s)	409, 674, 675, 929
BssMI	GATC	2 cut(s)	133, 582
BssT1I	CCWWGG	2 cut(s)	409, 929
Bst6I	CTCTTC	5 cut(s)	24, 119, 268, 824, 920
BstAFI	CTTAAG	1 cut(s)	737
BstAPI	GCANNNNNTGC	1 cut(s)	1316
BstBAI	YACGTR	1 cut(s)	211
BstC8I	GCNNGC	2 cut(s)	80, 734
BstDEI	CTNAG	5 cut(s)	237, 510, 586, 1476, 1549
BstDSI	CCRYGG	1 cut(s)	929
BstF5I	GGATG	3 cut(s)	67, 328, 436
BstH2I	RGCGCY	1 cut(s)	784
BstHHI	GCGC	1 cut(s)	783
BstKTI	GATC	2 cut(s)	136, 585
BstMAI	GTCTC	1 cut(s)	902
BstMBI	GATC	2 cut(s)	133, 582
BstMWI	GCNNNNNNNGC	6 cut(s)	233, 686, 742, 959, 1316, 1505
BstNSI	RCATGY	2 cut(s)	1057, 1085
BstSCI	CCNGG	2 cut(s)	673, 674
BstSFI	CTRYAG	1 cut(s)	900
BstSLI	GKGCMC	6 cut(s)	8, 84, 149, 216, 529, 682
BstV1I	GCAGC	2 cut(s)	589, 806
BstX2I	RGATCY	1 cut(s)	582
BstXI	CCANNNNNNTGG	1 cut(s)	427
BstYI	RGATCY	1 cut(s)	582
Bsu36I	CCTNAGG	1 cut(s)	1476
BsuRI	GGCC	5 cut(s)	407, 501, 680, 934, 953
BtgI	CCRYGG	1 cut(s)	929
BtrI	CACGTC	1 cut(s)	284
BtsCI	GGATG	3 cut(s)	67, 328, 436
BtsIMutI	CAGTG	5 cut(s)	149, 571, 835, 1254, 1419
BveI	ACCTGC	1 cut(s)	325
Cac8I	GCNNGC	2 cut(s)	80, 734
CfoI	GCGC	1 cut(s)	783
Cfr13I	GGNCC	7 cut(s)	406, 499, 561, 678, 679, 718, 951
Cfr9I	CCCGGG	1 cut(s)	674
CspCI	CAANNNNNGTGG	2 cut(s)	952, 987
DdeI	CTNAG	5 cut(s)	237, 510, 586, 1476, 1549
DpnI	GATC	2 cut(s)	135, 584
DpnII	GATC	2 cut(s)	133, 582
EaeI	YGGCCR	1 cut(s)	932
Eam1104I	CTCTTC	5 cut(s)	24, 119, 268, 824, 920
EarI	CTCTTC	5 cut(s)	24, 119, 268, 824, 920
EciI	GGCGGA	1 cut(s)	1523
Ecl136II	GAGCTC	1 cut(s)	835
Eco130I	CCWWGG	2 cut(s)	409, 929
Eco24I	GRGCYC	3 cut(s)	682, 837, 1036
Eco47I	GGWCC	2 cut(s)	561, 718
Eco53kI	GAGCTC	1 cut(s)	835
Eco57I	CTGAAG	1 cut(s)	459
Eco81I	CCTNAGG	1 cut(s)	1476
Eco88I	CYCGRG	2 cut(s)	674, 1136
EcoICRI	GAGCTC	1 cut(s)	835
EcoO109I	RGGNCCY	4 cut(s)	406, 678, 679, 718
EcoT14I	CCWWGG	2 cut(s)	409, 929
EcoT22I	ATGCAT	1 cut(s)	208
EcoT38I	GRGCYC	3 cut(s)	682, 837, 1036
ErhI	CCWWGG	2 cut(s)	409, 929
FalI	AAGNNNNNCTT	2 cut(s)	216, 248
FaqI	GGGAC	1 cut(s)	1400
FauI	CCCGC	2 cut(s)	71, 1164
FblI	GTMKAC	3 cut(s)	591, 855, 1071
Fnu4HI	GCNGC	2 cut(s)	603, 795
FokI	GGATG	3 cut(s)	74, 315, 443
FriOI	GRGCYC	3 cut(s)	682, 837, 1036
Fsp4HI	GCNGC	2 cut(s)	603, 795
FspBI	CTAG	4 cut(s)	137, 746, 1037, 1436
GlaI	GCGC	1 cut(s)	782
GluI	GCNGC	2 cut(s)	603, 795
GsuI	CTGGAG	1 cut(s)	560
HaeII	RGCGCY	1 cut(s)	784
HaeIII	GGCC	5 cut(s)	407, 501, 680, 934, 953
HapII	CCGG	1 cut(s)	675
HhaI	GCGC	1 cut(s)	783
Hin6I	GCGC	1 cut(s)	781
HinP1I	GCGC	1 cut(s)	781
HincII	GTYRAC	1 cut(s)	856
HindII	GTYRAC	1 cut(s)	856
HinfI	GANTC	6 cut(s)	416, 481, 755, 910, 1214, 1583
HpaII	CCGG	1 cut(s)	675
HphI	GGTGA	7 cut(s)	183, 268, 293, 425, 814, 1190, 1504
Hpy166II	GTNNAC	5 cut(s)	592, 856, 1011, 1072, 1348
Hpy188I	TCNGA	3 cut(s)	1219, 1377, 1588
Hpy188III	TCNNGA	2 cut(s)	658, 1136
Hpy8I	GTNNAC	5 cut(s)	592, 856, 1011, 1072, 1348
HpyAV	CCTTC	4 cut(s)	166, 776, 848, 1299
HpyCH4IV	ACGT	2 cut(s)	210, 283
HpyCH4V	TGCA	3 cut(s)	206, 320, 1319
HpyF10VI	GCNNNNNNNGC	6 cut(s)	233, 686, 742, 959, 1316, 1505
HpyF3I	CTNAG	5 cut(s)	237, 510, 586, 1476, 1549
HpySE526I	ACGT	2 cut(s)	210, 283
HspAI	GCGC	1 cut(s)	781
Kzo9I	GATC	2 cut(s)	133, 582
LmnI	GCTCC	1 cut(s)	840
Lsp1109I	GCAGC	2 cut(s)	589, 806
LweI	GCATC	1 cut(s)	193
MaeI	CTAG	4 cut(s)	137, 746, 1037, 1436
MaeII	ACGT	2 cut(s)	210, 283
MaeIII	GTNAC	4 cut(s)	189, 299, 608, 1015
MalI	GATC	2 cut(s)	135, 584
MboI	GATC	2 cut(s)	133, 582
MboII	GAAGA	9 cut(s)	41, 106, 285, 811, 833, 907, 1198, 1201, 1526
MfeI	CAATTG	1 cut(s)	339
MflI	RGATCY	1 cut(s)	582
MhlI	GDGCHC	8 cut(s)	8, 84, 149, 216, 529, 682, 837, 1036
MlsI	TGGCCA	1 cut(s)	934
MluNI	TGGCCA	1 cut(s)	934
MlyI	GAGTC	1 cut(s)	904
MmeI	TCCRAC	1 cut(s)	1569
Mox20I	TGGCCA	1 cut(s)	934
Mph1103I	ATGCAT	1 cut(s)	208
MscI	TGGCCA	1 cut(s)	934
MseI	TTAA	7 cut(s)	380, 738, 1103, 1194, 1371, 1424, 1529
MslI	CAYNNNNRTG	1 cut(s)	849
Msp20I	TGGCCA	1 cut(s)	934
MspA1I	CMGCKG	1 cut(s)	1159
MspCI	CTTAAG	1 cut(s)	737
MspI	CCGG	1 cut(s)	675
MspR9I	CCNGG	2 cut(s)	675, 676
MunI	CAATTG	1 cut(s)	339
Mva1269I	GAATGC	2 cut(s)	1393, 1582
MwoI	GCNNNNNNNGC	6 cut(s)	233, 686, 742, 959, 1316, 1505
NciI	CCSGG	2 cut(s)	675, 676
NcoI	CCATGG	1 cut(s)	929
NdeII	GATC	2 cut(s)	133, 582
NlaIV	GGNNCC	4 cut(s)	315, 679, 680, 719
NmuCI	GTSAC	3 cut(s)	189, 299, 1015
NsiI	ATGCAT	1 cut(s)	208
NspI	RCATGY	2 cut(s)	1057, 1085
PaeR7I	CTCGAG	1 cut(s)	1136
PciI	ACATGT	1 cut(s)	1081
PctI	GAATGC	2 cut(s)	1393, 1582
PfeI	GAWTC	5 cut(s)	416, 481, 755, 1214, 1583
PflMI	CCANNNNNTGG	1 cut(s)	310
PkrI	GCNGC	2 cut(s)	604, 796
PleI	GAGTC	1 cut(s)	904
PpsI	GAGTC	1 cut(s)	904
Ppu21I	YACGTR	1 cut(s)	211
PpuMI	RGGWCCY	1 cut(s)	718
PscI	ACATGT	1 cut(s)	1081
PshBI	ATTAAT	1 cut(s)	1371
Psp124BI	GAGCTC	1 cut(s)	837
Psp5II	RGGWCCY	1 cut(s)	718
PspN4I	GGNNCC	4 cut(s)	315, 679, 680, 719
PspOMI	GGGCCC	1 cut(s)	678
PspPI	GGNCC	7 cut(s)	406, 499, 561, 678, 679, 718, 951
PspPPI	RGGWCCY	1 cut(s)	718
PsrI	GAACNNNNNNTAC	2 cut(s)	306, 338
PsuI	RGATCY	1 cut(s)	582
RseI	CAYNNNNRTG	1 cut(s)	849
SacI	GAGCTC	1 cut(s)	837
SalI	GTCGAC	1 cut(s)	854
SaqAI	TTAA	7 cut(s)	380, 738, 1103, 1194, 1371, 1424, 1529
SatI	GCNGC	2 cut(s)	603, 795
Sau3AI	GATC	2 cut(s)	133, 582
Sau96I	GGNCC	7 cut(s)	406, 499, 561, 678, 679, 718, 951
SchI	GAGTC	1 cut(s)	904
ScrFI	CCNGG	2 cut(s)	675, 676
SduI	GDGCHC	8 cut(s)	8, 84, 149, 216, 529, 682, 837, 1036
SfaNI	GCATC	1 cut(s)	193
SfcI	CTRYAG	1 cut(s)	900
Sfr274I	CTCGAG	1 cut(s)	1136
SinI	GGWCC	2 cut(s)	561, 718
SlaI	CTCGAG	1 cut(s)	1136
SmaI	CCCGGG	1 cut(s)	676
SmiMI	CAYNNNNRTG	1 cut(s)	849
SmlI	CTYRAG	3 cut(s)	486, 737, 1136
SmoI	CTYRAG	3 cut(s)	486, 737, 1136
SsiI	CCGC	4 cut(s)	78, 1157, 1440, 1508
SspMI	CTAG	4 cut(s)	137, 746, 1037, 1436
SstI	GAGCTC	1 cut(s)	837
StyD4I	CCNGG	2 cut(s)	673, 674
StyI	CCWWGG	2 cut(s)	409, 929
TaiI	ACGT	2 cut(s)	213, 286
TaqI	TCGA	3 cut(s)	127, 855, 1137
TfiI	GAWTC	5 cut(s)	416, 481, 755, 1214, 1583
Tru1I	TTAA	7 cut(s)	380, 738, 1103, 1194, 1371, 1424, 1529
Tru9I	TTAA	7 cut(s)	380, 738, 1103, 1194, 1371, 1424, 1529
TscAI	CASTG	5 cut(s)	149, 571, 835, 1254, 1419
TseFI	GTSAC	3 cut(s)	189, 299, 1015
TseI	GCWGC	2 cut(s)	602, 794
Tsp45I	GTSAC	3 cut(s)	189, 299, 1015
TspDTI	ATGAA	5 cut(s)	447, 833, 853, 1215, 1553
TspGWI	ACGGA	4 cut(s)	38, 93, 436, 1223
TspMI	CCCGGG	1 cut(s)	674
TspRI	CASTG	5 cut(s)	149, 571, 835, 1254, 1419
Van91I	CCANNNNNTGG	1 cut(s)	310
Vha464I	CTTAAG	1 cut(s)	737
VpaK11BI	GGWCC	2 cut(s)	561, 718
VspI	ATTAAT	1 cut(s)	1371
XapI	RAATTY	1 cut(s)	662
XceI	RCATGY	2 cut(s)	1057, 1085
XhoI	CTCGAG	1 cut(s)	1136
XmaI	CCCGGG	1 cut(s)	674
XmiI	GTMKAC	3 cut(s)	591, 855, 1071
XspI	CTAG	4 cut(s)	137, 746, 1037, 1436
Zsp2I	ATGCAT	1 cut(s)	208

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.