Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0006257

Physical Location & Seq

Genomic Coordinates Reverse (-)

3277361 .. 3288883

Gene Structure 11523 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1026700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1365 bp

ATGTTTACTCTCTCTGCAACGCCATCCACCGCATTCCTCACGCCTAGGATTTCGCCGTCGTCTCTTTCCTCCTCCGCCTCCGCTGCTTACTCTTCGTCACCGTCGTCTTCTTCTCGGCTGTGCTTGGTCTCTTTGAGGTGGCGGAGCTCCGCCTGTCCTTGCTTGTTTTCCACCTTGAAGGTGACTGCCATGGCGGAGCTGGTGAAGGACACGGAGTCGGCTTCTTTGTCGCCCATTCAGTGTGCAGAGAAGAGGGAGACTAACCATTCTCGCACCTTTCTCAACGCGACCACTGAACAAGAGCTGCTGTCTGCAATAAGGAAGGAAACAGAAGCTGGCAGGCTTCCCTCAACTGTTGCTTCAGGAATGGAAGAATTATATCAGAATTATAAAAATGCTATTCTTCAAAGCGGAAATCCCAAGGCAGATGAGATTGTGTTGTCAAATATGACTGCTGTGTTGGATCGTGTATTCTTGGATATAGAGGATCCATTTATCTTCTCACCATATCACAAGGCAATGCGAGAGCCTTTTGATTACTACATGTTTGGTCAAAACTATATTCGTCCTTTAGTTGATTTCAGAAACTCATATGTTGGCAACCATTCCATTTTCTATGAAATTGAAGAGAAGCTTCAGCAGGGTCACAACATCTTTTTGATATCAAACCACCAAACTGAAGCAGATCCAGCTGTCATTGCCTTGTTGCTTGAATCAACAAACCCACATCTCGCTGAGAAAATGACCTACATAGCAGGAGATAGGGTTTTGACAGATCCTCTTTGTAAGCCTTTCAGCATGGGGAGGAATCTGATATGTGTATACTCCAAAAAGCACATGAATGATGTTCCTGAGCTTGTTGATATGAAAAGAAAAGCAAACACACGGAGTTTGAAGGAAATGGCAGTGCTATTGAGGGGCGGGTCACAGATTGTATGGATTGCACCTAGTGGTGGCAGGGACCGGCCAGATCCTGCAACAGGAGAATGGTACCCAGCACCTTTTGATGCTGCTTCACTGGACAATCTAAGAAGGCTTGGTGAACATTCTGCTGCGCCAGGGCATATATATCCCTTAGCATTGTTGTGTCACAACATCATGCCCCCTCCACCCCAGGTAGAAAAAGAAATTGGAGAAAGGAGAATGATCTCCTTCCATGGTACTGGATTATCGGTTGCACCAGAAATCACCTTCTCTGATATTACTGCTTCCTGTCAAAATTCTGAAGAGGCGAGGGAGGTATACACACAAGCTCTGTACGGTATCGTTACTGAACATTACAATGTGCTGAAAGCTGCTATACATTACAAACAAGGACTAAACGCATCCACTCCAAATGTTTCTTTGTCACAACCGTGGAGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004366 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006629 GO:0006644 GO:0006650 GO:0006655 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0008374 GO:0008610 GO:0008654 GO:0009058 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016740 GO:0016746 GO:0016747 GO:0019637 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045017 GO:0046471 GO:0046474 GO:0046486 GO:0071704 GO:0090407 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 map00561 map00564 map01100 map01110

Pfam Domains

Protein Families

Acyltransferase GPAT_N

Protein Analysis

455

Amino Acids

50.38

Weight (kDa)

6.13

Isoelectric Point (pI)

60.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GPAT_N	PF14829	90 - 165	1.9e-37	Glycerol-3-phosphate acyltransferase N-terminal
Acyltransferase	PF01553	204 - 360	3.9e-15	Acyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006257)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G32200 AT1G32200
fragaria_vesca	FvH4_5g23060 FvH4_5g23060 FvH4_5g23060 FvH4_5g23060
malus_domestica	MD06G1026700.v1.1 MD16G1279400.v1.1
prunus_persica	Prupe.5G027200_v2.0.a1 Prupe.5G027200_v2.0.a1 Prupe.5G027200_v2.0.a1 Prupe.5G027200_v2.0.a1 Prupe.5G027200_v2.0.a1 Prupe.5G027200_v2.0.a1 Prupe.5G027200_v2.0.a1 Prupe.5G027200_v2.0.a1
pyrus_communis	pycom06g02180 pycom16g24970
rosa_chinensis	RchiOBHm_Chr7g0211781
rosa_multiflora	Rmu_sc0016160.1_g000003
rosa_roxburghii	Rroxscaffold_3G00247450
rosa_rugosa	Rorug07G0128600
rosa_samantha	Rh7AG261300 Rh7BG254900 Rh7CG277900 Rh7DG267900
rosa_wichuraiana	Rw7G022270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	390
Acc65I	GGTACC	1 cut(s)	990
AccB1I	GGYRCC	1 cut(s)	990
AccI	GTMKAC	2 cut(s)	822, 1242
AccII	CGCG	1 cut(s)	287
AciI	CCGC	8 cut(s)	30, 75, 81, 142, 150, 194, 411, 921
AclWI	GGATC	6 cut(s)	471, 482, 495, 680, 770, 965
AcoI	YGGCCR	1 cut(s)	965
AcsI	RAATTY	1 cut(s)	1219
AcuI	CTGAAG	4 cut(s)	345, 620, 699, 1245
AdeI	CACNNNGTG	1 cut(s)	950
AfaI	GTAC	3 cut(s)	992, 1162, 1259
AfiI	CCNNNNNNNGG	2 cut(s)	953, 980
AflIII	ACRYGT	1 cut(s)	543
AgsI	TTSAA	5 cut(s)	178, 407, 626, 713, 895
AhdI	GACNNNNNGTC	1 cut(s)	214
AjnI	CCWGG	2 cut(s)	1057, 1113
AjuI	GAANNNNNNNTTGG	2 cut(s)	1113, 1145
AleI	CACNNNNGTG	1 cut(s)	1354
AluBI	AGCT	9 cut(s)	147, 199, 304, 335, 634, 692, 856, 1253, 1295
AluI	AGCT	9 cut(s)	147, 199, 304, 335, 634, 692, 856, 1253, 1295
Alw21I	GWGCWC	1 cut(s)	149
Alw26I	GTCTC	3 cut(s)	66, 133, 251
AlwI	GGATC	6 cut(s)	471, 482, 495, 680, 770, 965
AlwNI	CAGNNNCTG	2 cut(s)	335, 974
AoxI	GGCC	1 cut(s)	965
ApeKI	GCWGC	5 cut(s)	83, 304, 1010, 1052, 1295
ApoI	RAATTY	1 cut(s)	1219
Asp718I	GGTACC	1 cut(s)	990
AspA2I	CCTAGG	1 cut(s)	44
AspLEI	GCGC	1 cut(s)	1057
AspS9I	GGNCC	1 cut(s)	961
AsuHPI	GGTGA	6 cut(s)	90, 193, 214, 495, 1052, 1180
AvaII	GGWCC	1 cut(s)	961
AvrII	CCTAGG	1 cut(s)	44
BamHI	GGATCC	1 cut(s)	487
BanI	GGYRCC	1 cut(s)	990
BanII	GRGCYC	1 cut(s)	149
BbsI	GAAGAC	1 cut(s)	99
Bbv12I	GWGCWC	1 cut(s)	149
BbvI	GCAGC	5 cut(s)	70, 291, 997, 1039, 1282
BccI	CCATC	1 cut(s)	31
BceAI	ACGGC	1 cut(s)	40
BciT130I	CCWGG	2 cut(s)	1059, 1115
BcoDI	GTCTC	3 cut(s)	66, 133, 251
BfaI	CTAG	2 cut(s)	45, 948
BisI	GCNGC	5 cut(s)	84, 305, 1011, 1053, 1296
BlnI	CCTAGG	1 cut(s)	44
BlsI	GCNGC	5 cut(s)	85, 306, 1012, 1054, 1297
Bme1390I	CCNGG	2 cut(s)	1059, 1115
Bme18I	GGWCC	1 cut(s)	961
BmeRI	GACNNNNNGTC	1 cut(s)	214
BmgT120I	GGNCC	1 cut(s)	961
BmiI	GGNNCC	3 cut(s)	489, 962, 992
BmrFI	CCNGG	2 cut(s)	1059, 1115
BmsI	GCATC	2 cut(s)	997, 1334
BpiI	GAAGAC	1 cut(s)	99
Bpu10I	CCTNAGC	2 cut(s)	852, 1075
BsaI	GGTCTC	1 cut(s)	133
BsaJI	CCNNGG	7 cut(s)	44, 189, 420, 1058, 1113, 1156, 1355
BsaXI	ACNNNNNCTCC	2 cut(s)	750, 780
Bsc4I	CCNNNNNNNGG	2 cut(s)	953, 980
Bse118I	RCCGGY	1 cut(s)	963
Bse1I	ACTGG	2 cut(s)	1023, 1168
Bse3DI	GCAATG	2 cut(s)	525, 696
BseBI	CCWGG	2 cut(s)	1059, 1115
BseDI	CCNNGG	7 cut(s)	44, 189, 420, 1058, 1113, 1156, 1355
BseGI	GGATG	2 cut(s)	23, 1325
BseLI	CCNNNNNNNGG	2 cut(s)	953, 980
BseMI	GCAATG	2 cut(s)	525, 696
BseMII	CTCAG	2 cut(s)	726, 843
BseNI	ACTGG	2 cut(s)	1023, 1168
BseRI	GAGGAG	1 cut(s)	61
BseXI	GCAGC	5 cut(s)	70, 291, 997, 1039, 1282
BseYI	CCCAGC	1 cut(s)	994
BsgI	GTGCAG	1 cut(s)	264
Bsh1236I	CGCG	1 cut(s)	287
BshFI	GGCC	1 cut(s)	967
BshNI	GGYRCC	1 cut(s)	990
BsiHKAI	GWGCWC	1 cut(s)	149
BsiSI	CCGG	1 cut(s)	964
BslFI	GGGAC	1 cut(s)	974
BslI	CCNNNNNNNGG	2 cut(s)	953, 980
BsmAI	GTCTC	3 cut(s)	66, 133, 251
BsmBI	CGTCTC	1 cut(s)	66
BsmFI	GGGAC	1 cut(s)	974
BsmI	GAATGC	1 cut(s)	32
BsnI	GGCC	1 cut(s)	967
Bso31I	GGTCTC	1 cut(s)	133
Bsp1286I	GDGCHC	1 cut(s)	149
Bsp143I	GATC	6 cut(s)	463, 487, 685, 775, 970, 1146
Bsp19I	CCATGG	2 cut(s)	189, 1156
BspACI	CCGC	8 cut(s)	30, 75, 81, 142, 150, 194, 411, 921
BspANI	GGCC	1 cut(s)	967
BspCNI	CTCAG	2 cut(s)	727, 844
BspFNI	CGCG	1 cut(s)	287
BspLI	GGNNCC	3 cut(s)	489, 962, 992
BspPI	GGATC	6 cut(s)	471, 482, 495, 680, 770, 965
BspT107I	GGYRCC	1 cut(s)	990
BspTNI	GGTCTC	1 cut(s)	133
BsrDI	GCAATG	2 cut(s)	525, 696
BsrFI	RCCGGY	1 cut(s)	963
BsrI	ACTGG	2 cut(s)	1023, 1168
BssAI	RCCGGY	1 cut(s)	963
BssECI	CCNNGG	7 cut(s)	44, 189, 420, 1058, 1113, 1156, 1355
BssMI	GATC	6 cut(s)	463, 487, 685, 775, 970, 1146
BssNAI	GTATAC	2 cut(s)	823, 1243
BssT1I	CCWWGG	4 cut(s)	44, 189, 420, 1156
Bst1107I	GTATAC	2 cut(s)	823, 1243
Bst2UI	CCWGG	2 cut(s)	1059, 1115
Bst4CI	ACNGT	4 cut(s)	102, 355, 1262, 1356
Bst6I	CTCTTC	4 cut(s)	97, 245, 621, 1221
BstC8I	GCNNGC	2 cut(s)	337, 341
BstDEI	CTNAG	4 cut(s)	735, 852, 1028, 1075
BstDSI	CCRYGG	3 cut(s)	189, 1156, 1355
BstENI	CCTNNNNNAGG	1 cut(s)	978
BstF5I	GGATG	2 cut(s)	23, 1325
BstFNI	CGCG	1 cut(s)	287
BstHHI	GCGC	1 cut(s)	1057
BstKTI	GATC	6 cut(s)	466, 490, 688, 778, 973, 1149
BstMAI	GTCTC	3 cut(s)	66, 133, 251
BstMBI	GATC	6 cut(s)	463, 487, 685, 775, 970, 1146
BstMWI	GCNNNNNNNGC	3 cut(s)	83, 689, 698
BstNI	CCWGG	2 cut(s)	1059, 1115
BstNSI	RCATGY	1 cut(s)	547
BstSCI	CCNGG	2 cut(s)	1057, 1113
BstUI	CGCG	1 cut(s)	287
BstV1I	GCAGC	5 cut(s)	70, 291, 997, 1039, 1282
BstV2I	GAAGAC	1 cut(s)	99
BstX2I	RGATCY	4 cut(s)	487, 685, 775, 970
BstXI	CCANNNNNNTGG	1 cut(s)	1163
BstYI	RGATCY	4 cut(s)	487, 685, 775, 970
BstZ17I	GTATAC	2 cut(s)	823, 1243
BsuRI	GGCC	1 cut(s)	967
BtgI	CCRYGG	3 cut(s)	189, 1156, 1355
BtsCI	GGATG	2 cut(s)	23, 1325
BtsI	GCAGTG	1 cut(s)	912
BtsIMutI	CAGTG	4 cut(s)	245, 291, 912, 1016
Cac8I	GCNNGC	2 cut(s)	337, 341
CaiI	CAGNNNCTG	2 cut(s)	335, 974
CfoI	GCGC	1 cut(s)	1057
Cfr10I	RCCGGY	1 cut(s)	963
Cfr13I	GGNCC	1 cut(s)	961
Csp6I	GTAC	3 cut(s)	991, 1161, 1258
CviAII	CATG	6 cut(s)	190, 544, 799, 838, 1099, 1157
CviQI	GTAC	3 cut(s)	991, 1161, 1258
DdeI	CTNAG	4 cut(s)	735, 852, 1028, 1075
DpnI	GATC	6 cut(s)	465, 489, 687, 777, 972, 1148
DpnII	GATC	6 cut(s)	463, 487, 685, 775, 970, 1146
DraIII	CACNNNGTG	1 cut(s)	950
DriI	GACNNNNNGTC	1 cut(s)	214
EaeI	YGGCCR	1 cut(s)	965
Eam1104I	CTCTTC	4 cut(s)	97, 245, 621, 1221
Eam1105I	GACNNNNNGTC	1 cut(s)	214
EarI	CTCTTC	4 cut(s)	97, 245, 621, 1221
EciI	GGCGGA	4 cut(s)	64, 139, 157, 209
Ecl136II	GAGCTC	1 cut(s)	147
Eco130I	CCWWGG	4 cut(s)	44, 189, 420, 1156
Eco24I	GRGCYC	1 cut(s)	149
Eco31I	GGTCTC	1 cut(s)	133
Eco32I	GATATC	1 cut(s)	663
Eco47I	GGWCC	1 cut(s)	961
Eco53kI	GAGCTC	1 cut(s)	147
Eco57I	CTGAAG	4 cut(s)	345, 620, 699, 1245
EcoICRI	GAGCTC	1 cut(s)	147
EcoNI	CCTNNNNNAGG	1 cut(s)	978
EcoRII	CCWGG	2 cut(s)	1057, 1113
EcoRV	GATATC	1 cut(s)	663
EcoT14I	CCWWGG	4 cut(s)	44, 189, 420, 1156
EcoT38I	GRGCYC	1 cut(s)	149
ErhI	CCWWGG	4 cut(s)	44, 189, 420, 1156
Esp3I	CGTCTC	1 cut(s)	66
FaeI	CATG	6 cut(s)	193, 547, 802, 841, 1102, 1160
FalI	AAGNNNNNCTT	2 cut(s)	618, 650
FaqI	GGGAC	1 cut(s)	974
FatI	CATG	6 cut(s)	189, 543, 798, 837, 1098, 1156
FauI	CCCGC	1 cut(s)	914
FauNDI	CATATG	1 cut(s)	592
FblI	GTMKAC	2 cut(s)	822, 1242
Fnu4HI	GCNGC	5 cut(s)	84, 305, 1011, 1053, 1296
FokI	GGATG	2 cut(s)	10, 1312
FriOI	GRGCYC	1 cut(s)	149
Fsp4HI	GCNGC	5 cut(s)	84, 305, 1011, 1053, 1296
FspBI	CTAG	2 cut(s)	45, 948
GlaI	GCGC	1 cut(s)	1056
GluI	GCNGC	5 cut(s)	84, 305, 1011, 1053, 1296
GsaI	CCCAGC	1 cut(s)	998
HaeIII	GGCC	1 cut(s)	967
HapII	CCGG	1 cut(s)	964
HhaI	GCGC	1 cut(s)	1057
Hin1II	CATG	6 cut(s)	193, 547, 802, 841, 1102, 1160
Hin6I	GCGC	1 cut(s)	1055
HinP1I	GCGC	1 cut(s)	1055
HindIII	AAGCTT	1 cut(s)	632
HinfI	GANTC	3 cut(s)	215, 713, 808
HpaII	CCGG	1 cut(s)	964
HphI	GGTGA	6 cut(s)	90, 193, 214, 495, 1052, 1180
Hpy166II	GTNNAC	4 cut(s)	6, 823, 1043, 1243
Hpy188I	TCNGA	5 cut(s)	384, 584, 813, 1198, 1225
Hpy188III	TCNNGA	2 cut(s)	363, 851
Hpy8I	GTNNAC	4 cut(s)	6, 823, 1043, 1243
Hpy99I	CGWCG	2 cut(s)	61, 106
HpyAV	CCTTC	7 cut(s)	172, 199, 316, 889, 1026, 1162, 1201
HpyCH4III	ACNGT	4 cut(s)	102, 355, 1262, 1356
HpyCH4V	TGCA	6 cut(s)	17, 245, 314, 944, 977, 1178
HpyF10VI	GCNNNNNNNGC	3 cut(s)	83, 689, 698
HpyF3I	CTNAG	4 cut(s)	735, 852, 1028, 1075
Hsp92II	CATG	6 cut(s)	193, 547, 802, 841, 1102, 1160
HspAI	GCGC	1 cut(s)	1055
KpnI	GGTACC	1 cut(s)	994
Kzo9I	GATC	6 cut(s)	463, 487, 685, 775, 970, 1146
LmnI	GCTCC	3 cut(s)	144, 152, 196
Lsp1109I	GCAGC	5 cut(s)	70, 291, 997, 1039, 1282
LweI	GCATC	2 cut(s)	997, 1334
MaeI	CTAG	2 cut(s)	45, 948
MaeIII	GTNAC	7 cut(s)	96, 181, 644, 924, 1088, 1267, 1347
MalI	GATC	6 cut(s)	465, 489, 687, 777, 972, 1148
MboI	GATC	6 cut(s)	463, 487, 685, 775, 970, 1146
MboII	GAAGA	9 cut(s)	84, 99, 102, 262, 383, 395, 490, 638, 1238
MflI	RGATCY	4 cut(s)	487, 685, 775, 970
MhlI	GDGCHC	1 cut(s)	149
MluCI	AATT	5 cut(s)	374, 385, 621, 1128, 1219
MlyI	GAGTC	1 cut(s)	224
MmeI	TCCRAC	1 cut(s)	441
MslI	CAYNNNNRTG	4 cut(s)	840, 1084, 1281, 1354
MspA1I	CMGCKG	2 cut(s)	83, 692
MspI	CCGG	1 cut(s)	964
MspR9I	CCNGG	2 cut(s)	1059, 1115
Mva1269I	GAATGC	1 cut(s)	32
MvaI	CCWGG	2 cut(s)	1059, 1115
MvnI	CGCG	1 cut(s)	287
MwoI	GCNNNNNNNGC	3 cut(s)	83, 689, 698
NcoI	CCATGG	2 cut(s)	189, 1156
NdeI	CATATG	1 cut(s)	592
NdeII	GATC	6 cut(s)	463, 487, 685, 775, 970, 1146
NlaIII	CATG	6 cut(s)	193, 547, 802, 841, 1102, 1160
NlaIV	GGNNCC	3 cut(s)	489, 962, 992
NmeAIII	GCCGAG	1 cut(s)	94
NmuCI	GTSAC	6 cut(s)	96, 181, 644, 924, 1088, 1347
NspI	RCATGY	1 cut(s)	547
OliI	CACNNNNGTG	1 cut(s)	1354
PciI	ACATGT	1 cut(s)	543
PcsI	WCGNNNNNNNCGW	1 cut(s)	101
PctI	GAATGC	1 cut(s)	32
PfeI	GAWTC	2 cut(s)	713, 808
PkrI	GCNGC	5 cut(s)	85, 306, 1012, 1054, 1297
PleI	GAGTC	1 cut(s)	223
PpsI	GAGTC	1 cut(s)	223
PscI	ACATGT	1 cut(s)	543
PsiI	TTATAA	1 cut(s)	390
Psp124BI	GAGCTC	1 cut(s)	149
Psp6I	CCWGG	2 cut(s)	1057, 1113
PspFI	CCCAGC	1 cut(s)	994
PspGI	CCWGG	2 cut(s)	1057, 1113
PspN4I	GGNNCC	3 cut(s)	489, 962, 992
PspPI	GGNCC	1 cut(s)	961
PstNI	CAGNNNCTG	2 cut(s)	335, 974
PsuI	RGATCY	4 cut(s)	487, 685, 775, 970
PvuII	CAGCTG	1 cut(s)	692
RsaI	GTAC	3 cut(s)	992, 1162, 1259
RsaNI	GTAC	3 cut(s)	991, 1161, 1258
RseI	CAYNNNNRTG	4 cut(s)	840, 1084, 1281, 1354
SacI	GAGCTC	1 cut(s)	149
SatI	GCNGC	5 cut(s)	84, 305, 1011, 1053, 1296
Sau3AI	GATC	6 cut(s)	463, 487, 685, 775, 970, 1146
Sau96I	GGNCC	1 cut(s)	961
SchI	GAGTC	1 cut(s)	224
ScrFI	CCNGG	2 cut(s)	1059, 1115
SduI	GDGCHC	1 cut(s)	149
SfaNI	GCATC	2 cut(s)	997, 1334
SinI	GGWCC	1 cut(s)	961
SmiMI	CAYNNNNRTG	4 cut(s)	840, 1084, 1281, 1354
Sse9I	AATT	5 cut(s)	374, 385, 621, 1128, 1219
SsiI	CCGC	8 cut(s)	30, 75, 81, 142, 150, 194, 411, 921
SspMI	CTAG	2 cut(s)	45, 948
SstI	GAGCTC	1 cut(s)	149
StyD4I	CCNGG	2 cut(s)	1057, 1113
StyI	CCWWGG	4 cut(s)	44, 189, 420, 1156
TaaI	ACNGT	4 cut(s)	102, 355, 1262, 1356
TasI	AATT	5 cut(s)	374, 385, 621, 1128, 1219
TfiI	GAWTC	2 cut(s)	713, 808
TscAI	CASTG	4 cut(s)	245, 298, 912, 1023
TseFI	GTSAC	6 cut(s)	96, 181, 644, 924, 1088, 1347
TseI	GCWGC	5 cut(s)	83, 304, 1010, 1052, 1295
Tsp45I	GTSAC	6 cut(s)	96, 181, 644, 924, 1088, 1347
TspDTI	ATGAA	3 cut(s)	633, 854, 881
TspGWI	ACGGA	2 cut(s)	227, 901
TspRI	CASTG	4 cut(s)	245, 298, 912, 1023
VpaK11BI	GGWCC	1 cut(s)	961
XagI	CCTNNNNNAGG	1 cut(s)	978
XapI	RAATTY	1 cut(s)	1219
XceI	RCATGY	1 cut(s)	547
XcmI	CCANNNNNNNNNTGG	1 cut(s)	196
XmaJI	CCTAGG	1 cut(s)	44
XmiI	GTMKAC	2 cut(s)	822, 1242
XspI	CTAG	2 cut(s)	45, 948

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.