Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0006257

Physical Location & Seq

Genomic Coordinates Forward (+)

37971108 .. 37989001

Gene Structure 17894 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1279400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1368 bp

ATGTTTACCCTCTCCGCGACGCCATCCACCACATTCCTCACGCCTAGGGTTTCGCCGTCGTCTCTTTCCTCCTCCTCCCCCTCCGCTGCTTCTTGTTCGTCACTGTCGTCGTCTTCTCGACTGTGCTTGGTCTCTGTGAGACGGCGGAGCTCTGCCTCTCCTTGCTTGTTTTCCTCCTTGAAGGTCAGTGCCATGGCGGAGCTGGTGAAGGACACGGAGTCGTTGTCTTTGTCGCCCATTCAGAGTGCAGAGAGGACGGAGACTGATCATTCTCGCACCTTTCTCAGTGCGACCACTGAACAAGAGCTGTTGTCTGTAATAAGGAAGGAAACAGAAGCTGGCAGGCTTCCCTCAACTATTGCATCAGGAATGGAAGAATTATATCAGAATTATAAAAATGCTATTCTTCAAAGCAGAAATCCCAAGGCAGATGAGACTGTGTTGTCAAATATGACTGCTGTATTGGATCGTGTATTCTTGGATATAGAGGACCCATTTGTCTTCTCACCATATCACAAAGCAATGCGAGTGCCTTTTGATTACTACATGTTTGGTCAAAACTATATTCGTCCTTTAGTTGATTTCAGAAACTCATATGTTGGCAATCTTTCCATTTTCTATGAAATTGAAGAGAAGCTTCAGCAGGGTCACAACATTTTTTTGATATCAAACCACCAAACTGAAGCAGATCCAGCTGTCATTGCCTTGTTGCTTGAATCAACAAACCCACATCTTGCTGAGAAAATGACCTACATAGCAGGAGATAGGGTTGTGACAGATCCTCTTTGTAAGCCTTTCAGCATGGGGAGGAATCTGATATGTGTGTACTCCAAAAAGCACATGAATGATGATCCTGAGCTTGCTGATATGAAAAGAAAAGCAAACACCCGGAGTTTGAAGGAAATGGCATTGCTATTGAGGGGCGGATCACAAATTGTATGGATTGCACCTAGTGGTGGAAGGGACCGCCCAGATCCTGCAACAGGAGAGTGGTACCCAGCACCTTTTGATGCTGCTTCACTGGACAATCTGAGAAGGCTTGGTGAACATTCTGCTGCTCCAGGGCATATATATCCCTTAGCATTATTGTGTCACAACATCATGCCTCCTCCAGCCCAGGTAGAAAAAGAAATTGGAGAAAAGAGAATGATCTCCTTCCATGGTACTGGATTATCAGTTGCACCAGAAATCACCTTCTCTGATATTACTGCTTCCTGTCAAAATTCTGAAGAGGCTAGGGAGGTATATGCACAAGCTCTGTATGATATTGTTACTGAACAGTACAATGTGCTCAAATCTGCTGTACATTATGAACAAGGACTAAACGCATCCACTCCAAATGTTTCTTTGTCGCAACCATGGAGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004366 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006629 GO:0006644 GO:0006650 GO:0006655 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0008374 GO:0008610 GO:0008654 GO:0009058 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016740 GO:0016746 GO:0016747 GO:0019637 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045017 GO:0046471 GO:0046474 GO:0046486 GO:0071704 GO:0090407 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 map00561 map00564 map01100 map01110

Pfam Domains

Protein Families

Acyltransferase GPAT_N

Protein Analysis

456

Amino Acids

50.36

Weight (kDa)

5.72

Isoelectric Point (pI)

63.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GPAT_N	PF14829	91 - 166	7.4e-36	Glycerol-3-phosphate acyltransferase N-terminal
Acyltransferase	PF01553	205 - 361	7.1e-15	Acyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006257)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G32200 AT1G32200
fragaria_vesca	FvH4_5g23060 FvH4_5g23060 FvH4_5g23060 FvH4_5g23060
malus_domestica	MD06G1026700.v1.1 MD16G1279400.v1.1
prunus_persica	Prupe.5G027200_v2.0.a1 Prupe.5G027200_v2.0.a1 Prupe.5G027200_v2.0.a1 Prupe.5G027200_v2.0.a1 Prupe.5G027200_v2.0.a1 Prupe.5G027200_v2.0.a1 Prupe.5G027200_v2.0.a1 Prupe.5G027200_v2.0.a1
pyrus_communis	pycom06g02180 pycom16g24970
rosa_chinensis	RchiOBHm_Chr7g0211781
rosa_multiflora	Rmu_sc0016160.1_g000003
rosa_roxburghii	Rroxscaffold_3G00247450
rosa_rugosa	Rorug07G0128600
rosa_samantha	Rh7AG261300 Rh7BG254900 Rh7CG277900 Rh7DG267900
rosa_wichuraiana	Rw7G022270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	393
AasI	GACNNNNNNGTC	2 cut(s)	442, 497
Acc65I	GGTACC	1 cut(s)	993
AccB1I	GGYRCC	1 cut(s)	993
AccII	CGCG	1 cut(s)	17
AciI	CCGC	6 cut(s)	15, 84, 145, 197, 924, 967
AclWI	GGATC	6 cut(s)	474, 683, 773, 845, 934, 968
AcsI	RAATTY	1 cut(s)	1222
AcuI	CTGAAG	3 cut(s)	623, 702, 1248
AcyI	GRCGYC	1 cut(s)	20
AdeI	CACNNNGTG	1 cut(s)	953
AfaI	GTAC	5 cut(s)	827, 995, 1165, 1283, 1305
AfiI	CCNNNNNNNGG	2 cut(s)	956, 983
AflIII	ACRYGT	1 cut(s)	546
AgsI	TTSAA	5 cut(s)	181, 410, 629, 716, 898
AhdI	GACNNNNNGTC	1 cut(s)	217
AjnI	CCWGG	2 cut(s)	1060, 1116
AjuI	GAANNNNNNNTTGG	2 cut(s)	1116, 1148
AluBI	AGCT	8 cut(s)	150, 202, 307, 338, 637, 695, 859, 1256
AluI	AGCT	8 cut(s)	150, 202, 307, 338, 637, 695, 859, 1256
Alw21I	GWGCWC	2 cut(s)	152, 1293
Alw26I	GTCTC	5 cut(s)	66, 133, 136, 254, 428
AlwI	GGATC	6 cut(s)	474, 683, 773, 845, 934, 968
AlwNI	CAGNNNCTG	2 cut(s)	338, 977
ApeKI	GCWGC	3 cut(s)	86, 1013, 1055
ApoI	RAATTY	1 cut(s)	1222
Asp718I	GGTACC	1 cut(s)	993
AspA2I	CCTAGG	1 cut(s)	44
AspS9I	GGNCC	2 cut(s)	490, 964
AsuC2I	CCSGG	1 cut(s)	889
AsuHPI	GGTGA	4 cut(s)	217, 498, 1055, 1183
AvaII	GGWCC	2 cut(s)	490, 964
AvrII	CCTAGG	1 cut(s)	44
BanI	GGYRCC	1 cut(s)	993
BanII	GRGCYC	1 cut(s)	152
BbsI	GAAGAC	2 cut(s)	105, 493
Bbv12I	GWGCWC	2 cut(s)	152, 1293
BbvI	GCAGC	3 cut(s)	73, 1000, 1042
BccI	CCATC	1 cut(s)	31
BceAI	ACGGC	2 cut(s)	40, 158
BciT130I	CCWGG	2 cut(s)	1062, 1118
BclI	TGATCA	1 cut(s)	265
BcnI	CCSGG	1 cut(s)	889
BcoDI	GTCTC	5 cut(s)	66, 133, 136, 254, 428
BfaI	CTAG	3 cut(s)	45, 951, 1236
BisI	GCNGC	3 cut(s)	87, 1014, 1056
BlnI	CCTAGG	1 cut(s)	44
BlsI	GCNGC	3 cut(s)	88, 1015, 1057
Bme1390I	CCNGG	3 cut(s)	889, 1062, 1118
Bme18I	GGWCC	2 cut(s)	490, 964
BmeRI	GACNNNNNGTC	1 cut(s)	217
BmgT120I	GGNCC	2 cut(s)	490, 964
BmiI	GGNNCC	3 cut(s)	492, 965, 995
BmrFI	CCNGG	3 cut(s)	889, 1062, 1118
BmsI	GCATC	3 cut(s)	371, 1000, 1337
BpiI	GAAGAC	2 cut(s)	105, 493
BpmI	CTGGAG	2 cut(s)	1044, 1095
Bpu10I	CCTNAGC	2 cut(s)	855, 1078
BpuMI	CCSGG	1 cut(s)	889
BsaHI	GRCGYC	1 cut(s)	20
BsaI	GGTCTC	1 cut(s)	136
BsaJI	CCNNGG	7 cut(s)	44, 192, 423, 1061, 1116, 1159, 1358
BsaXI	ACNNNNNCTCC	4 cut(s)	209, 239, 753, 783
Bsc4I	CCNNNNNNNGG	2 cut(s)	956, 983
Bse1I	ACTGG	2 cut(s)	1026, 1171
Bse3DI	GCAATG	3 cut(s)	528, 699, 908
BseBI	CCWGG	2 cut(s)	1062, 1118
BseDI	CCNNGG	7 cut(s)	44, 192, 423, 1061, 1116, 1159, 1358
BseGI	GGATG	2 cut(s)	23, 1328
BseLI	CCNNNNNNNGG	2 cut(s)	956, 983
BseMI	GCAATG	3 cut(s)	528, 699, 908
BseMII	CTCAG	4 cut(s)	298, 729, 846, 1022
BseNI	ACTGG	2 cut(s)	1026, 1171
BseRI	GAGGAG	3 cut(s)	61, 64, 1098
BseXI	GCAGC	3 cut(s)	73, 1000, 1042
BseYI	CCCAGC	1 cut(s)	997
BsgI	GTGCAG	1 cut(s)	267
Bsh1236I	CGCG	1 cut(s)	17
BshNI	GGYRCC	1 cut(s)	993
BsiHKAI	GWGCWC	2 cut(s)	152, 1293
BsiSI	CCGG	1 cut(s)	889
BslFI	GGGAC	1 cut(s)	977
BslI	CCNNNNNNNGG	2 cut(s)	956, 983
BsmAI	GTCTC	5 cut(s)	66, 133, 136, 254, 428
BsmBI	CGTCTC	2 cut(s)	66, 133
BsmFI	GGGAC	1 cut(s)	977
Bso31I	GGTCTC	1 cut(s)	136
Bsp1286I	GDGCHC	2 cut(s)	152, 1293
Bsp1407I	TGTACA	1 cut(s)	1303
Bsp143I	GATC	8 cut(s)	265, 466, 688, 778, 850, 926, 973, 1149
Bsp19I	CCATGG	3 cut(s)	192, 1159, 1358
BspACI	CCGC	6 cut(s)	15, 84, 145, 197, 924, 967
BspCNI	CTCAG	4 cut(s)	297, 730, 847, 1023
BspFNI	CGCG	1 cut(s)	17
BspLI	GGNNCC	3 cut(s)	492, 965, 995
BspPI	GGATC	6 cut(s)	474, 683, 773, 845, 934, 968
BspT107I	GGYRCC	1 cut(s)	993
BspTNI	GGTCTC	1 cut(s)	136
BsrDI	GCAATG	3 cut(s)	528, 699, 908
BsrGI	TGTACA	1 cut(s)	1303
BsrI	ACTGG	2 cut(s)	1026, 1171
BssECI	CCNNGG	7 cut(s)	44, 192, 423, 1061, 1116, 1159, 1358
BssMI	GATC	8 cut(s)	265, 466, 688, 778, 850, 926, 973, 1149
BssNI	GRCGYC	1 cut(s)	20
BssT1I	CCWWGG	5 cut(s)	44, 192, 423, 1159, 1358
Bst2UI	CCWGG	2 cut(s)	1062, 1118
Bst4CI	ACNGT	4 cut(s)	105, 123, 439, 1281
Bst6I	CTCTTC	2 cut(s)	624, 1224
BstACI	GRCGYC	1 cut(s)	20
BstAUI	TGTACA	1 cut(s)	1303
BstC8I	GCNNGC	3 cut(s)	340, 344, 861
BstDEI	CTNAG	5 cut(s)	284, 738, 855, 1031, 1078
BstDSI	CCRYGG	3 cut(s)	192, 1159, 1358
BstENI	CCTNNNNNAGG	1 cut(s)	981
BstF5I	GGATG	2 cut(s)	23, 1328
BstFNI	CGCG	1 cut(s)	17
BstKTI	GATC	8 cut(s)	268, 469, 691, 781, 853, 929, 976, 1152
BstMAI	GTCTC	5 cut(s)	66, 133, 136, 254, 428
BstMBI	GATC	8 cut(s)	265, 466, 688, 778, 850, 926, 973, 1149
BstMWI	GCNNNNNNNGC	2 cut(s)	692, 701
BstNI	CCWGG	2 cut(s)	1062, 1118
BstNSI	RCATGY	1 cut(s)	550
BstSCI	CCNGG	3 cut(s)	887, 1060, 1116
BstUI	CGCG	1 cut(s)	17
BstV1I	GCAGC	3 cut(s)	73, 1000, 1042
BstV2I	GAAGAC	2 cut(s)	105, 493
BstX2I	RGATCY	3 cut(s)	688, 778, 973
BstXI	CCANNNNNNTGG	1 cut(s)	1166
BstYI	RGATCY	3 cut(s)	688, 778, 973
BtgI	CCRYGG	3 cut(s)	192, 1159, 1358
BtsCI	GGATG	2 cut(s)	23, 1328
BtsIMutI	CAGTG	5 cut(s)	101, 193, 292, 294, 1019
Cac8I	GCNNGC	3 cut(s)	340, 344, 861
CaiI	CAGNNNCTG	2 cut(s)	338, 977
Cfr13I	GGNCC	2 cut(s)	490, 964
CseI	GACGC	1 cut(s)	28
Csp6I	GTAC	5 cut(s)	826, 994, 1164, 1282, 1304
CviAII	CATG	7 cut(s)	193, 547, 802, 841, 1102, 1160, 1359
CviQI	GTAC	5 cut(s)	826, 994, 1164, 1282, 1304
DdeI	CTNAG	5 cut(s)	284, 738, 855, 1031, 1078
DpnI	GATC	8 cut(s)	267, 468, 690, 780, 852, 928, 975, 1151
DpnII	GATC	8 cut(s)	265, 466, 688, 778, 850, 926, 973, 1149
DraIII	CACNNNGTG	1 cut(s)	953
DrdI	GACNNNNNNGTC	2 cut(s)	442, 497
DriI	GACNNNNNGTC	1 cut(s)	217
DseDI	GACNNNNNNGTC	2 cut(s)	442, 497
Eam1104I	CTCTTC	2 cut(s)	624, 1224
Eam1105I	GACNNNNNGTC	1 cut(s)	217
EarI	CTCTTC	2 cut(s)	624, 1224
EciI	GGCGGA	3 cut(s)	160, 212, 939
Ecl136II	GAGCTC	1 cut(s)	150
Eco130I	CCWWGG	5 cut(s)	44, 192, 423, 1159, 1358
Eco24I	GRGCYC	1 cut(s)	152
Eco31I	GGTCTC	1 cut(s)	136
Eco32I	GATATC	1 cut(s)	666
Eco47I	GGWCC	2 cut(s)	490, 964
Eco53kI	GAGCTC	1 cut(s)	150
Eco57I	CTGAAG	3 cut(s)	623, 702, 1248
EcoICRI	GAGCTC	1 cut(s)	150
EcoNI	CCTNNNNNAGG	1 cut(s)	981
EcoO109I	RGGNCCY	1 cut(s)	490
EcoRII	CCWGG	2 cut(s)	1060, 1116
EcoRV	GATATC	1 cut(s)	666
EcoT14I	CCWWGG	5 cut(s)	44, 192, 423, 1159, 1358
EcoT38I	GRGCYC	1 cut(s)	152
ErhI	CCWWGG	5 cut(s)	44, 192, 423, 1159, 1358
Esp3I	CGTCTC	2 cut(s)	66, 133
FaeI	CATG	7 cut(s)	196, 550, 805, 844, 1105, 1163, 1362
FalI	AAGNNNNNCTT	2 cut(s)	621, 653
FaqI	GGGAC	1 cut(s)	977
FatI	CATG	7 cut(s)	192, 546, 801, 840, 1101, 1159, 1358
FauNDI	CATATG	1 cut(s)	595
FbaI	TGATCA	1 cut(s)	265
Fnu4HI	GCNGC	3 cut(s)	87, 1014, 1056
FokI	GGATG	2 cut(s)	10, 1315
FriOI	GRGCYC	1 cut(s)	152
Fsp4HI	GCNGC	3 cut(s)	87, 1014, 1056
FspBI	CTAG	3 cut(s)	45, 951, 1236
GluI	GCNGC	3 cut(s)	87, 1014, 1056
GsaI	CCCAGC	1 cut(s)	1001
GsuI	CTGGAG	2 cut(s)	1044, 1095
HapII	CCGG	1 cut(s)	889
HgaI	GACGC	1 cut(s)	28
Hin1I	GRCGYC	1 cut(s)	20
Hin1II	CATG	7 cut(s)	196, 550, 805, 844, 1105, 1163, 1362
HindIII	AAGCTT	1 cut(s)	635
HinfI	GANTC	3 cut(s)	218, 716, 811
HpaII	CCGG	1 cut(s)	889
HphI	GGTGA	4 cut(s)	217, 498, 1055, 1183
Hpy166II	GTNNAC	3 cut(s)	6, 826, 1046
Hpy188I	TCNGA	7 cut(s)	243, 387, 587, 816, 1032, 1201, 1228
Hpy188III	TCNNGA	3 cut(s)	117, 366, 854
Hpy8I	GTNNAC	3 cut(s)	6, 826, 1046
Hpy99I	CGWCG	3 cut(s)	22, 61, 112
HpyAV	CCTTC	8 cut(s)	175, 202, 319, 892, 954, 1029, 1165, 1204
HpyCH4III	ACNGT	4 cut(s)	105, 123, 439, 1281
HpyCH4V	TGCA	6 cut(s)	248, 362, 947, 980, 1181, 1250
HpyF10VI	GCNNNNNNNGC	2 cut(s)	692, 701
HpyF3I	CTNAG	5 cut(s)	284, 738, 855, 1031, 1078
Hsp92I	GRCGYC	1 cut(s)	20
Hsp92II	CATG	7 cut(s)	196, 550, 805, 844, 1105, 1163, 1362
KpnI	GGTACC	1 cut(s)	997
Ksp22I	TGATCA	1 cut(s)	265
Kzo9I	GATC	8 cut(s)	265, 466, 688, 778, 850, 926, 973, 1149
LmnI	GCTCC	3 cut(s)	147, 199, 1063
Lsp1109I	GCAGC	3 cut(s)	73, 1000, 1042
LweI	GCATC	3 cut(s)	371, 1000, 1337
MaeI	CTAG	3 cut(s)	45, 951, 1236
MaeIII	GTNAC	5 cut(s)	99, 647, 772, 1091, 1270
MalI	GATC	8 cut(s)	267, 468, 690, 780, 852, 928, 975, 1151
MboI	GATC	8 cut(s)	265, 466, 688, 778, 850, 926, 973, 1149
MboII	GAAGA	6 cut(s)	105, 386, 398, 493, 641, 1241
MflI	RGATCY	3 cut(s)	688, 778, 973
MhlI	GDGCHC	2 cut(s)	152, 1293
MluCI	AATT	6 cut(s)	377, 388, 624, 933, 1131, 1222
MlyI	GAGTC	1 cut(s)	227
MslI	CAYNNNNRTG	2 cut(s)	843, 1087
MspA1I	CMGCKG	2 cut(s)	86, 695
MspI	CCGG	1 cut(s)	889
MspR9I	CCNGG	3 cut(s)	889, 1062, 1118
MvaI	CCWGG	2 cut(s)	1062, 1118
MvnI	CGCG	1 cut(s)	17
MwoI	GCNNNNNNNGC	2 cut(s)	692, 701
NciI	CCSGG	1 cut(s)	889
NcoI	CCATGG	3 cut(s)	192, 1159, 1358
NdeI	CATATG	1 cut(s)	595
NdeII	GATC	8 cut(s)	265, 466, 688, 778, 850, 926, 973, 1149
NlaIII	CATG	7 cut(s)	196, 550, 805, 844, 1105, 1163, 1362
NlaIV	GGNNCC	3 cut(s)	492, 965, 995
NmuCI	GTSAC	4 cut(s)	99, 647, 772, 1091
NspI	RCATGY	1 cut(s)	550
PciI	ACATGT	1 cut(s)	546
PcsI	WCGNNNNNNNCGW	1 cut(s)	104
PfeI	GAWTC	2 cut(s)	716, 811
PkrI	GCNGC	3 cut(s)	88, 1015, 1057
PleI	GAGTC	1 cut(s)	226
PpsI	GAGTC	1 cut(s)	226
PpuMI	RGGWCCY	1 cut(s)	490
PscI	ACATGT	1 cut(s)	546
PsiI	TTATAA	1 cut(s)	393
Psp124BI	GAGCTC	1 cut(s)	152
Psp5II	RGGWCCY	1 cut(s)	490
Psp6I	CCWGG	2 cut(s)	1060, 1116
PspFI	CCCAGC	1 cut(s)	997
PspGI	CCWGG	2 cut(s)	1060, 1116
PspN4I	GGNNCC	3 cut(s)	492, 965, 995
PspPI	GGNCC	2 cut(s)	490, 964
PspPPI	RGGWCCY	1 cut(s)	490
PstNI	CAGNNNCTG	2 cut(s)	338, 977
PsuI	RGATCY	3 cut(s)	688, 778, 973
PvuII	CAGCTG	1 cut(s)	695
RsaI	GTAC	5 cut(s)	827, 995, 1165, 1283, 1305
RsaNI	GTAC	5 cut(s)	826, 994, 1164, 1282, 1304
RseI	CAYNNNNRTG	2 cut(s)	843, 1087
SacI	GAGCTC	1 cut(s)	152
SatI	GCNGC	3 cut(s)	87, 1014, 1056
Sau3AI	GATC	8 cut(s)	265, 466, 688, 778, 850, 926, 973, 1149
Sau96I	GGNCC	2 cut(s)	490, 964
SchI	GAGTC	1 cut(s)	227
ScrFI	CCNGG	3 cut(s)	889, 1062, 1118
SduI	GDGCHC	2 cut(s)	152, 1293
SfaNI	GCATC	3 cut(s)	371, 1000, 1337
SinI	GGWCC	2 cut(s)	490, 964
SmiMI	CAYNNNNRTG	2 cut(s)	843, 1087
Sse9I	AATT	6 cut(s)	377, 388, 624, 933, 1131, 1222
SsiI	CCGC	6 cut(s)	15, 84, 145, 197, 924, 967
SspMI	CTAG	3 cut(s)	45, 951, 1236
SstI	GAGCTC	1 cut(s)	152
StyD4I	CCNGG	3 cut(s)	887, 1060, 1116
StyI	CCWWGG	5 cut(s)	44, 192, 423, 1159, 1358
TaaI	ACNGT	4 cut(s)	105, 123, 439, 1281
TaqI	TCGA	1 cut(s)	118
TasI	AATT	6 cut(s)	377, 388, 624, 933, 1131, 1222
TatI	WGTACW	3 cut(s)	825, 1281, 1303
TfiI	GAWTC	2 cut(s)	716, 811
TscAI	CASTG	5 cut(s)	108, 193, 292, 301, 1026
TseFI	GTSAC	4 cut(s)	99, 647, 772, 1091
TseI	GCWGC	3 cut(s)	86, 1013, 1055
Tsp45I	GTSAC	4 cut(s)	99, 647, 772, 1091
TspDTI	ATGAA	4 cut(s)	636, 857, 884, 1326
TspGWI	ACGGA	2 cut(s)	230, 272
TspRI	CASTG	5 cut(s)	108, 193, 292, 301, 1026
VpaK11BI	GGWCC	2 cut(s)	490, 964
XagI	CCTNNNNNAGG	1 cut(s)	981
XapI	RAATTY	1 cut(s)	1222
XceI	RCATGY	1 cut(s)	550
XcmI	CCANNNNNNNNNTGG	1 cut(s)	199
XmaJI	CCTAGG	1 cut(s)	44
XspI	CTAG	3 cut(s)	45, 951, 1236

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.