MD06G1042700.v1.1

Lysine methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0016856
Physical Location & Seq
Reverse (-)
5596690 .. 5597361
672 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1042700.v1.1.491

Sequence Viewer

Length: 672 bp
ATGGGCACGAGACAAATCCAGGTTGCAGACCAAACCCTAGTTATCCACGAGCTAGACGACGTCAGTGACTCAACAACGGGTCGAGCTCTCACCGGCTCATGGGTTTGGGACTCGGCCATTGTCCTATCCAACTGGATGGCCACTCAAGGCGCAGTCCACTTTCACTTCCAAGATAAGTCCGTCCTCGAGCTCGGTGCTGGGGCAGGACTTCCCGGCCTGACAGCGGCTCGGCTTGGAGCGAGCCGAGTCGTTCTCACGGATATGGGGCCACTCCTTCCGGGGCTGTTGAGGAATGTGGAGGCCAACGGACTCGGGGACCGAGTCGAGGTGAGGGAACTCGTGTGGGGATCGGACGAGTCACCGAGTCAAATCAGCGAGTTCGACCTAATTTTGATGTCTGACGTGTTCTATAACCAAGAGGAAATGGTGGGGTTGGCCAAGACGTTGAAGAGCGTGTGCGGCAGAGGTACTACGGTTTGGGCGTCAACGGAGGTCAGGCAGTGGACCGCCGAGTGCTTAAGCGAGTTGGTACAGGAAGGTTTTGGACTCGTTGAGTTACCGAGTCAACTGGTGGAGGAAATGGAGGAGGGCTTGGATTGTTTTGCCATTTTCCAAGTCATACCACCACATGAAGATAACCAAAAAACTTTCATTGTCGAGGGCTCTATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_16

Protein Analysis

224

Amino Acids

24.31

Weight (kDa)

4.32

Isoelectric Point (pI)

34.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_16 PF10294 31 - 167 4.7e-29 Lysine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016856)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g20871
malus_domestica MD06G1042700.v1.1
pyrus_communis pycom06g03560
rosa_chinensis RchiOBHm_Chr7g0207961
rosa_laevigata RLG00000003269
rosa_multiflora Rmu_sc0000905.1_g000011
rosa_roxburghii Rroxscaffold_3G00250980
rosa_rugosa Rorug07G0100500
rosa_samantha Rh7AG235800 Rh7BG229700 Rh7CG249300 Rh7DG240200
rosa_wichuraiana Rw0G001250 Rw0G017670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 63
AciI CCGC 3 cut(s) 224, 459, 507
AclWI GGATC 1 cut(s) 355
AcoI YGGCCR 3 cut(s) 114, 138, 435
AcyI GRCGYC 2 cut(s) 60, 482
AfaI GTAC 2 cut(s) 469, 531
AfiI CCNNNNNNNGG 3 cut(s) 99, 223, 325
AflII CTTAAG 1 cut(s) 517
AflIII ACRYGT 1 cut(s) 402
AgsI TTSAA 1 cut(s) 448
AjiI CACGTC 1 cut(s) 403
AjnI CCWGG 1 cut(s) 18
AluBI AGCT 3 cut(s) 52, 86, 190
AluI AGCT 3 cut(s) 52, 86, 190
Alw21I GWGCWC 2 cut(s) 88, 192
Alw26I GTCTC 1 cut(s) 4
AlwI GGATC 1 cut(s) 355
Ama87I CYCGRG 2 cut(s) 185, 311
AoxI GGCC 6 cut(s) 114, 138, 214, 266, 300, 435
AspLEI GCGC 1 cut(s) 152
AspS9I GGNCC 3 cut(s) 266, 316, 504
AsuC2I CCSGG 2 cut(s) 213, 279
AsuHPI GGTGA 3 cut(s) 82, 340, 351
AvaI CYCGRG 2 cut(s) 185, 311
AvaII GGWCC 2 cut(s) 316, 504
BaeGI GKGCMC 1 cut(s) 8
BaeI ACNNNNGTAYC 2 cut(s) 459, 492
BalI TGGCCA 2 cut(s) 140, 437
BanII GRGCYC 3 cut(s) 88, 192, 665
BauI CACGAG 3 cut(s) 7, 47, 338
Bbv12I GWGCWC 2 cut(s) 88, 192
BccI CCATC 1 cut(s) 130
BcgI CGANNNNNNTGC 2 cut(s) 176, 210
BciT130I CCWGG 1 cut(s) 20
BcnI CCSGG 2 cut(s) 213, 279
BcoDI GTCTC 1 cut(s) 4
BfaI CTAG 2 cut(s) 38, 53
BfrI CTTAAG 1 cut(s) 517
BisI GCNGC 2 cut(s) 225, 460
BlsI GCNGC 2 cut(s) 226, 461
Bme1390I CCNGG 3 cut(s) 20, 213, 279
Bme18I GGWCC 2 cut(s) 316, 504
BmeT110I CYCGRG 2 cut(s) 185, 311
BmgBI CACGTC 1 cut(s) 403
BmgT120I GGNCC 3 cut(s) 266, 316, 504
BmiI GGNNCC 2 cut(s) 267, 317
BmrFI CCNGG 3 cut(s) 20, 213, 279
BplI GAGNNNNNCTC 2 cut(s) 237, 269
BpuEI CTTGAG 1 cut(s) 129
BpuMI CCSGG 2 cut(s) 213, 279
BsaHI GRCGYC 2 cut(s) 60, 482
BsaJI CCNNGG 1 cut(s) 278
Bsc4I CCNNNNNNNGG 3 cut(s) 99, 223, 325
Bse118I RCCGGY 1 cut(s) 92
Bse1I ACTGG 2 cut(s) 137, 573
BseBI CCWGG 1 cut(s) 20
BseDI CCNNGG 1 cut(s) 278
BseGI GGATG 1 cut(s) 141
BseLI CCNNNNNNNGG 3 cut(s) 99, 223, 325
BseNI ACTGG 2 cut(s) 137, 573
BseRI GAGGAG 1 cut(s) 599
BseSI GKGCMC 1 cut(s) 8
BseYI CCCAGC 1 cut(s) 197
BshFI GGCC 6 cut(s) 116, 140, 216, 268, 302, 437
BsiHKAI GWGCWC 2 cut(s) 88, 192
BsiHKCI CYCGRG 2 cut(s) 185, 311
BsiSI CCGG 3 cut(s) 93, 213, 278
BslFI GGGAC 2 cut(s) 122, 329
BslI CCNNNNNNNGG 3 cut(s) 99, 223, 325
BsmAI GTCTC 1 cut(s) 4
BsmFI GGGAC 2 cut(s) 122, 329
BsnI GGCC 6 cut(s) 116, 140, 216, 268, 302, 437
BsoBI CYCGRG 2 cut(s) 185, 311
Bsp1286I GDGCHC 4 cut(s) 8, 88, 192, 665
Bsp143I GATC 1 cut(s) 347
BspACI CCGC 3 cut(s) 224, 459, 507
BspANI GGCC 6 cut(s) 116, 140, 216, 268, 302, 437
BspLI GGNNCC 2 cut(s) 267, 317
BspPI GGATC 1 cut(s) 355
BspQI GCTCTTC 1 cut(s) 443
BspTI CTTAAG 1 cut(s) 517
BsrFI RCCGGY 1 cut(s) 92
BsrI ACTGG 2 cut(s) 137, 573
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 1 cut(s) 278
BssMI GATC 1 cut(s) 347
BssNI GRCGYC 2 cut(s) 60, 482
BssSI CACGAG 3 cut(s) 7, 47, 338
Bst2BI CACGAG 3 cut(s) 7, 47, 338
Bst2UI CCWGG 1 cut(s) 20
Bst4CI ACNGT 1 cut(s) 475
Bst6I CTCTTC 1 cut(s) 443
BstACI GRCGYC 2 cut(s) 60, 482
BstAFI CTTAAG 1 cut(s) 517
BstC8I GCNNGC 1 cut(s) 241
BstF5I GGATG 1 cut(s) 141
BstHHI GCGC 1 cut(s) 152
BstKTI GATC 1 cut(s) 350
BstMAI GTCTC 1 cut(s) 4
BstMBI GATC 1 cut(s) 347
BstMWI GCNNNNNNNGC 1 cut(s) 459
BstNI CCWGG 1 cut(s) 20
BstSCI CCNGG 3 cut(s) 18, 211, 277
BstSLI GKGCMC 1 cut(s) 8
BstXI CCANNNNNNTGG 1 cut(s) 136
BsuRI GGCC 6 cut(s) 116, 140, 216, 268, 302, 437
BtrI CACGTC 1 cut(s) 403
BtsCI GGATG 1 cut(s) 141
BtsI GCAGTG 1 cut(s) 506
BtsIMutI CAGTG 2 cut(s) 70, 506
Cac8I GCNNGC 1 cut(s) 241
CfoI GCGC 1 cut(s) 152
Cfr10I RCCGGY 1 cut(s) 92
Cfr13I GGNCC 3 cut(s) 266, 316, 504
CseI GACGC 1 cut(s) 471
Csp6I GTAC 2 cut(s) 468, 530
CviAII CATG 2 cut(s) 99, 629
CviQI GTAC 2 cut(s) 468, 530
DpnI GATC 1 cut(s) 349
DpnII GATC 1 cut(s) 347
EaeI YGGCCR 3 cut(s) 114, 138, 435
Eam1104I CTCTTC 1 cut(s) 443
EarI CTCTTC 1 cut(s) 443
Ecl136II GAGCTC 2 cut(s) 86, 190
Eco24I GRGCYC 3 cut(s) 88, 192, 665
Eco47I GGWCC 2 cut(s) 316, 504
Eco53kI GAGCTC 2 cut(s) 86, 190
Eco88I CYCGRG 2 cut(s) 185, 311
EcoICRI GAGCTC 2 cut(s) 86, 190
EcoRII CCWGG 1 cut(s) 18
EcoT38I GRGCYC 3 cut(s) 88, 192, 665
FaeI CATG 2 cut(s) 102, 632
FaiI YATR 5 cut(s) 100, 263, 411, 620, 630
FaqI GGGAC 2 cut(s) 122, 329
FatI CATG 2 cut(s) 98, 628
Fnu4HI GCNGC 2 cut(s) 225, 460
FokI GGATG 1 cut(s) 148
FriOI GRGCYC 3 cut(s) 88, 192, 665
Fsp4HI GCNGC 2 cut(s) 225, 460
FspBI CTAG 2 cut(s) 38, 53
GlaI GCGC 1 cut(s) 151
GluI GCNGC 2 cut(s) 225, 460
GsaI CCCAGC 1 cut(s) 201
HaeIII GGCC 6 cut(s) 116, 140, 216, 268, 302, 437
HapII CCGG 3 cut(s) 93, 213, 278
HgaI GACGC 1 cut(s) 471
HhaI GCGC 1 cut(s) 152
Hin1I GRCGYC 2 cut(s) 60, 482
Hin1II CATG 2 cut(s) 102, 632
Hin6I GCGC 1 cut(s) 150
HinP1I GCGC 1 cut(s) 150
HincII GTYRAC 2 cut(s) 486, 566
HindII GTYRAC 2 cut(s) 486, 566
HinfI GANTC 9 cut(s) 68, 110, 246, 309, 321, 356, 364, 546, 562
HpaII CCGG 3 cut(s) 93, 213, 278
HphI GGTGA 3 cut(s) 82, 340, 351
Hpy166II GTNNAC 4 cut(s) 157, 486, 504, 566
Hpy188I TCNGA 2 cut(s) 352, 400
Hpy8I GTNNAC 4 cut(s) 157, 486, 504, 566
Hpy99I CGWCG 1 cut(s) 62
HpyAV CCTTC 2 cut(s) 284, 530
HpyCH4III ACNGT 1 cut(s) 475
HpyCH4IV ACGT 3 cut(s) 60, 402, 443
HpyCH4V TGCA 1 cut(s) 26
HpyF10VI GCNNNNNNNGC 1 cut(s) 459
HpySE526I ACGT 3 cut(s) 60, 402, 443
Hsp92I GRCGYC 2 cut(s) 60, 482
Hsp92II CATG 2 cut(s) 102, 632
HspAI GCGC 1 cut(s) 150
Kzo9I GATC 1 cut(s) 347
LguI GCTCTTC 1 cut(s) 443
LmnI GCTCC 1 cut(s) 236
MaeI CTAG 2 cut(s) 38, 53
MaeII ACGT 3 cut(s) 60, 402, 443
MaeIII GTNAC 3 cut(s) 65, 357, 555
MalI GATC 1 cut(s) 349
MboI GATC 1 cut(s) 347
MboII GAAGA 2 cut(s) 460, 644
MhlI GDGCHC 4 cut(s) 8, 88, 192, 665
MlsI TGGCCA 2 cut(s) 140, 437
MluCI AATT 1 cut(s) 387
MluNI TGGCCA 2 cut(s) 140, 437
MlyI GAGTC 9 cut(s) 62, 104, 255, 303, 330, 365, 373, 540, 571
MmeI TCCRAC 1 cut(s) 153
Mox20I TGGCCA 2 cut(s) 140, 437
MscI TGGCCA 2 cut(s) 140, 437
MseI TTAA 1 cut(s) 518
MslI CAYNNNNRTG 1 cut(s) 260
Msp20I TGGCCA 2 cut(s) 140, 437
MspA1I CMGCKG 1 cut(s) 224
MspCI CTTAAG 1 cut(s) 517
MspI CCGG 3 cut(s) 93, 213, 278
MspR9I CCNGG 3 cut(s) 20, 213, 279
MvaI CCWGG 1 cut(s) 20
MwoI GCNNNNNNNGC 1 cut(s) 459
NciI CCSGG 2 cut(s) 213, 279
NdeII GATC 1 cut(s) 347
NlaIII CATG 2 cut(s) 102, 632
NlaIV GGNNCC 2 cut(s) 267, 317
NmeAIII GCCGAG 4 cut(s) 92, 208, 269, 535
NmuCI GTSAC 2 cut(s) 65, 357
PaeR7I CTCGAG 1 cut(s) 185
PciSI GCTCTTC 1 cut(s) 443
PcsI WCGNNNNNNNCGW 2 cut(s) 54, 479
PflFI GACNNNGTC 2 cut(s) 59, 320
PkrI GCNGC 2 cut(s) 226, 461
PleI GAGTC 9 cut(s) 62, 104, 254, 303, 329, 364, 372, 540, 570
PpsI GAGTC 9 cut(s) 62, 104, 254, 303, 329, 364, 372, 540, 570
Psp124BI GAGCTC 2 cut(s) 88, 192
Psp6I CCWGG 1 cut(s) 18
PspFI CCCAGC 1 cut(s) 197
PspGI CCWGG 1 cut(s) 18
PspN4I GGNNCC 2 cut(s) 267, 317
PspPI GGNCC 3 cut(s) 266, 316, 504
PspXI VCTCGAGB 1 cut(s) 185
PsyI GACNNNGTC 2 cut(s) 59, 320
RsaI GTAC 2 cut(s) 469, 531
RsaNI GTAC 2 cut(s) 468, 530
RseI CAYNNNNRTG 1 cut(s) 260
SacI GAGCTC 2 cut(s) 88, 192
SapI GCTCTTC 1 cut(s) 443
SaqAI TTAA 1 cut(s) 518
SatI GCNGC 2 cut(s) 225, 460
Sau3AI GATC 1 cut(s) 347
Sau96I GGNCC 3 cut(s) 266, 316, 504
SchI GAGTC 9 cut(s) 62, 104, 255, 303, 330, 365, 373, 540, 571
ScrFI CCNGG 3 cut(s) 20, 213, 279
SduI GDGCHC 4 cut(s) 8, 88, 192, 665
Sfr274I CTCGAG 1 cut(s) 185
SinI GGWCC 2 cut(s) 316, 504
SlaI CTCGAG 1 cut(s) 185
SmiMI CAYNNNNRTG 1 cut(s) 260
SmlI CTYRAG 3 cut(s) 144, 185, 517
SmoI CTYRAG 3 cut(s) 144, 185, 517
Sse9I AATT 1 cut(s) 387
SsiI CCGC 3 cut(s) 224, 459, 507
SspMI CTAG 2 cut(s) 38, 53
SstI GAGCTC 2 cut(s) 88, 192
StyD4I CCNGG 3 cut(s) 18, 211, 277
TaaI ACNGT 1 cut(s) 475
TaiI ACGT 3 cut(s) 63, 405, 446
TaqI TCGA 5 cut(s) 82, 186, 324, 381, 657
TaqII GACCGA 1 cut(s) 333
TasI AATT 1 cut(s) 387
TauI GCSGC 2 cut(s) 227, 462
Tru1I TTAA 1 cut(s) 518
Tru9I TTAA 1 cut(s) 518
TscAI CASTG 2 cut(s) 70, 506
TseFI GTSAC 2 cut(s) 65, 357
Tsp45I GTSAC 2 cut(s) 65, 357
TspDTI ATGAA 2 cut(s) 640, 645
TspGWI ACGGA 4 cut(s) 169, 272, 321, 503
TspRI CASTG 2 cut(s) 70, 506
Tth111I GACNNNGTC 2 cut(s) 59, 320
Vha464I CTTAAG 1 cut(s) 517
VpaK11BI GGWCC 2 cut(s) 316, 504
XhoI CTCGAG 1 cut(s) 185
XspI CTAG 2 cut(s) 38, 53
ZraI GACGTC 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.