Rroxscaffold_3G00250980

Lysine methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0016856
Physical Location & Seq
Reverse (-)
44841617 .. 44842303
687 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00250980.1

Sequence Viewer

Length: 687 bp
ATGGCCATGAGACAAATCCAAGTCGCCGACGACACGCTTGTAATCCATGAGCTCGAGGACGTTTGTGACCCGGCCACTGGCCGAGCACTCACCGGCTCATGGATTTGGGCCTCGGCTTTGATCCTATCCAACTGGATGACCAACCAAAGCCGAACAAACTTCAGTTTTCTAGGCAAGACCGTGCTTGAGCTTGGTGCAGGAGCTGGACTCCCCGGCTTGACCGCAGCCCGGCTAGGAGCGAGCCAAGTTGTGCTCACCGATATCAAGCCGCTGCTTCCAGGTTTGCTGAAGAATGTCGAAGCCAACGGACTTGGTGAACAAGTCAAGGTCAGGGAACTCATGTGGGGATCAGACGAGTCATTGAGCGAAGTCGGTGAGTTTGACTTGGTTTTGATGTCAGATGTGTTCTACAACGCCGAGGAGATGGTTGGCTTGGCCAAGACGTTGAAATGTGTGTGCAACAGAAAGACGACAGTTTTGGCAGCAACTGAGGTAAGGCCCTCAACAGCTGAGTGCCTGAGCGAGTTTGTTAGAGAAGGCTTTATGCTTGTTGAGTTGCCGAGTGAGTTCGCTTCTTTAGAAGGAATGGACACGGAGAGTTTGGATTCCTTTTCCATCTTCCAAGTCATACCACCGCATGAAGAAGGCCAAATGGATAGCCCTTGCAATCGAGACCACAGGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_16

Protein Analysis

228

Amino Acids

24.88

Weight (kDa)

4.5

Isoelectric Point (pI)

31.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_16 PF10294 31 - 168 1.3e-29 Lysine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016856)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g20871
malus_domestica MD06G1042700.v1.1
pyrus_communis pycom06g03560
rosa_chinensis RchiOBHm_Chr7g0207961
rosa_laevigata RLG00000003269
rosa_multiflora Rmu_sc0000905.1_g000011
rosa_roxburghii Rroxscaffold_3G00250980
rosa_rugosa Rorug07G0100500
rosa_samantha Rh7AG235800 Rh7BG229700 Rh7CG249300 Rh7DG240200
rosa_wichuraiana Rw0G001250 Rw0G017670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 222, 269, 635
AclWI GGATC 2 cut(s) 115, 355
AcoI YGGCCR 4 cut(s) 3, 72, 79, 435
AcuI CTGAAG 2 cut(s) 145, 308
AfiI CCNNNNNNNGG 3 cut(s) 77, 99, 228
AgsI TTSAA 1 cut(s) 448
AjnI CCWGG 1 cut(s) 277
AluBI AGCT 4 cut(s) 52, 190, 203, 509
AluI AGCT 4 cut(s) 52, 190, 203, 509
Alw21I GWGCWC 3 cut(s) 54, 88, 255
Alw26I GTCTC 2 cut(s) 4, 666
AlwI GGATC 2 cut(s) 115, 355
AlwNI CAGNNNCTG 2 cut(s) 203, 488
Ama87I CYCGRG 1 cut(s) 53
AoxI GGCC 7 cut(s) 3, 72, 79, 108, 435, 497, 646
ApeKI GCWGC 3 cut(s) 224, 271, 482
ArsI GACNNNNNNTTYG 2 cut(s) 460, 492
AspS9I GGNCC 2 cut(s) 108, 498
AsuC2I CCSGG 3 cut(s) 71, 213, 229
AsuHPI GGTGA 4 cut(s) 82, 247, 326, 386
AvaI CYCGRG 1 cut(s) 53
BalI TGGCCA 2 cut(s) 5, 437
BanII GRGCYC 1 cut(s) 54
Bbv12I GWGCWC 3 cut(s) 54, 88, 255
BbvI GCAGC 3 cut(s) 236, 258, 494
BccI CCATC 2 cut(s) 418, 623
BciT130I CCWGG 1 cut(s) 279
BcnI CCSGG 3 cut(s) 71, 213, 229
BcoDI GTCTC 2 cut(s) 4, 666
BfaI CTAG 2 cut(s) 170, 233
BisI GCNGC 4 cut(s) 225, 269, 272, 483
BlsI GCNGC 4 cut(s) 226, 270, 273, 484
Bme1390I CCNGG 4 cut(s) 71, 213, 229, 279
BmeT110I CYCGRG 1 cut(s) 53
BmgT120I GGNCC 2 cut(s) 108, 498
BmrFI CCNGG 4 cut(s) 71, 213, 229, 279
BplI GAGNNNNNCTC 2 cut(s) 192, 224
Bpu10I CCTNAGC 1 cut(s) 518
BpuEI CTTGAG 1 cut(s) 206
BpuMI CCSGG 3 cut(s) 71, 213, 229
BsaI GGTCTC 1 cut(s) 666
BsaJI CCNNGG 3 cut(s) 111, 211, 417
Bsc4I CCNNNNNNNGG 3 cut(s) 77, 99, 228
Bse118I RCCGGY 1 cut(s) 92
Bse1I ACTGG 2 cut(s) 82, 137
BseBI CCWGG 1 cut(s) 279
BseDI CCNNGG 3 cut(s) 111, 211, 417
BseGI GGATG 1 cut(s) 141
BseLI CCNNNNNNNGG 3 cut(s) 77, 99, 228
BseMII CTCAG 3 cut(s) 480, 501, 509
BseNI ACTGG 2 cut(s) 82, 137
BseRI GAGGAG 1 cut(s) 434
BseXI GCAGC 3 cut(s) 236, 258, 494
BsgI GTGCAG 1 cut(s) 216
BshFI GGCC 7 cut(s) 5, 74, 81, 110, 437, 499, 648
BsiHKAI GWGCWC 3 cut(s) 54, 88, 255
BsiHKCI CYCGRG 1 cut(s) 53
BsiSI CCGG 4 cut(s) 71, 93, 213, 229
BslI CCNNNNNNNGG 3 cut(s) 77, 99, 228
BsmAI GTCTC 2 cut(s) 4, 666
BsnI GGCC 7 cut(s) 5, 74, 81, 110, 437, 499, 648
Bso31I GGTCTC 1 cut(s) 666
BsoBI CYCGRG 1 cut(s) 53
Bsp1286I GDGCHC 3 cut(s) 54, 88, 255
Bsp143I GATC 2 cut(s) 120, 347
BspACI CCGC 3 cut(s) 222, 269, 635
BspANI GGCC 7 cut(s) 5, 74, 81, 110, 437, 499, 648
BspCNI CTCAG 3 cut(s) 481, 502, 510
BspPI GGATC 2 cut(s) 115, 355
BspTNI GGTCTC 1 cut(s) 666
BsrFI RCCGGY 1 cut(s) 92
BsrI ACTGG 2 cut(s) 82, 137
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 3 cut(s) 111, 211, 417
BssMI GATC 2 cut(s) 120, 347
Bst2UI CCWGG 1 cut(s) 279
Bst4CI ACNGT 2 cut(s) 181, 475
BstC8I GCNNGC 1 cut(s) 241
BstDEI CTNAG 3 cut(s) 489, 510, 518
BstF5I GGATG 1 cut(s) 141
BstKTI GATC 2 cut(s) 123, 350
BstMAI GTCTC 2 cut(s) 4, 666
BstMBI GATC 2 cut(s) 120, 347
BstNI CCWGG 1 cut(s) 279
BstSCI CCNGG 4 cut(s) 69, 211, 227, 277
BstV1I GCAGC 3 cut(s) 236, 258, 494
BsuRI GGCC 7 cut(s) 5, 74, 81, 110, 437, 499, 648
BtsCI GGATG 1 cut(s) 141
BtsIMutI CAGTG 1 cut(s) 75
Cac8I GCNNGC 1 cut(s) 241
CaiI CAGNNNCTG 2 cut(s) 203, 488
Cfr10I RCCGGY 1 cut(s) 92
Cfr13I GGNCC 2 cut(s) 108, 498
CspCI CAANNNNNGTGG 1 cut(s) 665
CviAII CATG 5 cut(s) 7, 47, 99, 340, 638
DdeI CTNAG 3 cut(s) 489, 510, 518
DpnI GATC 2 cut(s) 122, 349
DpnII GATC 2 cut(s) 120, 347
EaeI YGGCCR 4 cut(s) 3, 72, 79, 435
Ecl136II GAGCTC 1 cut(s) 52
Eco24I GRGCYC 1 cut(s) 54
Eco31I GGTCTC 1 cut(s) 666
Eco32I GATATC 1 cut(s) 262
Eco53kI GAGCTC 1 cut(s) 52
Eco57I CTGAAG 2 cut(s) 145, 308
Eco88I CYCGRG 1 cut(s) 53
EcoICRI GAGCTC 1 cut(s) 52
EcoO109I RGGNCCY 1 cut(s) 498
EcoRII CCWGG 1 cut(s) 277
EcoRV GATATC 1 cut(s) 262
EcoT38I GRGCYC 1 cut(s) 54
FaeI CATG 5 cut(s) 10, 50, 102, 343, 641
FaiI YATR 7 cut(s) 8, 48, 100, 341, 545, 629, 639
FatI CATG 5 cut(s) 6, 46, 98, 339, 637
Fnu4HI GCNGC 4 cut(s) 225, 269, 272, 483
FokI GGATG 1 cut(s) 148
FriOI GRGCYC 1 cut(s) 54
Fsp4HI GCNGC 4 cut(s) 225, 269, 272, 483
FspBI CTAG 2 cut(s) 170, 233
GluI GCNGC 4 cut(s) 225, 269, 272, 483
HaeIII GGCC 7 cut(s) 5, 74, 81, 110, 437, 499, 648
HapII CCGG 4 cut(s) 71, 93, 213, 229
Hin1II CATG 5 cut(s) 10, 50, 102, 343, 641
HinfI GANTC 3 cut(s) 207, 356, 605
HpaII CCGG 4 cut(s) 71, 93, 213, 229
HphI GGTGA 4 cut(s) 82, 247, 326, 386
Hpy166II GTNNAC 1 cut(s) 317
Hpy188I TCNGA 2 cut(s) 352, 400
Hpy188III TCNNGA 1 cut(s) 671
Hpy8I GTNNAC 1 cut(s) 317
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 3 cut(s) 530, 575, 638
HpyCH4III ACNGT 2 cut(s) 181, 475
HpyCH4IV ACGT 2 cut(s) 60, 443
HpyCH4V TGCA 3 cut(s) 197, 459, 666
HpyF3I CTNAG 3 cut(s) 489, 510, 518
HpySE526I ACGT 2 cut(s) 60, 443
Hsp92II CATG 5 cut(s) 10, 50, 102, 343, 641
Kzo9I GATC 2 cut(s) 120, 347
LmnI GCTCC 2 cut(s) 200, 236
Lsp1109I GCAGC 3 cut(s) 236, 258, 494
MaeI CTAG 2 cut(s) 170, 233
MaeII ACGT 2 cut(s) 60, 443
MaeIII GTNAC 1 cut(s) 65
MalI GATC 2 cut(s) 122, 349
MboI GATC 2 cut(s) 120, 347
MboII GAAGA 3 cut(s) 301, 610, 653
MhlI GDGCHC 3 cut(s) 54, 88, 255
MlsI TGGCCA 2 cut(s) 5, 437
MluNI TGGCCA 2 cut(s) 5, 437
MlyI GAGTC 2 cut(s) 201, 365
MmeI TCCRAC 1 cut(s) 153
MnlI CCTC 5 cut(s) 49, 121, 412, 484, 511
Mox20I TGGCCA 2 cut(s) 5, 437
MscI TGGCCA 2 cut(s) 5, 437
Msp20I TGGCCA 2 cut(s) 5, 437
MspA1I CMGCKG 2 cut(s) 271, 509
MspI CCGG 4 cut(s) 71, 93, 213, 229
MspR9I CCNGG 4 cut(s) 71, 213, 229, 279
MvaI CCWGG 1 cut(s) 279
NciI CCSGG 3 cut(s) 71, 213, 229
NdeII GATC 2 cut(s) 120, 347
NlaIII CATG 5 cut(s) 10, 50, 102, 343, 641
NmeAIII GCCGAG 4 cut(s) 92, 107, 442, 585
NmuCI GTSAC 1 cut(s) 65
PaeR7I CTCGAG 1 cut(s) 53
PfeI GAWTC 1 cut(s) 605
PkrI GCNGC 4 cut(s) 226, 270, 273, 484
PleI GAGTC 2 cut(s) 201, 364
PpsI GAGTC 2 cut(s) 201, 364
Psp124BI GAGCTC 1 cut(s) 54
Psp6I CCWGG 1 cut(s) 277
PspGI CCWGG 1 cut(s) 277
PspPI GGNCC 2 cut(s) 108, 498
PspXI VCTCGAGB 1 cut(s) 53
PstNI CAGNNNCTG 2 cut(s) 203, 488
PvuII CAGCTG 1 cut(s) 509
SacI GAGCTC 1 cut(s) 54
SatI GCNGC 4 cut(s) 225, 269, 272, 483
Sau3AI GATC 2 cut(s) 120, 347
Sau96I GGNCC 2 cut(s) 108, 498
SchI GAGTC 2 cut(s) 201, 365
ScrFI CCNGG 4 cut(s) 71, 213, 229, 279
SduI GDGCHC 3 cut(s) 54, 88, 255
SetI ASST 9 cut(s) 54, 63, 192, 205, 283, 330, 446, 495, 511
Sfr274I CTCGAG 1 cut(s) 53
SlaI CTCGAG 1 cut(s) 53
SmlI CTYRAG 2 cut(s) 53, 185
SmoI CTYRAG 2 cut(s) 53, 185
SsiI CCGC 3 cut(s) 222, 269, 635
SspMI CTAG 2 cut(s) 170, 233
SstI GAGCTC 1 cut(s) 54
StyD4I CCNGG 4 cut(s) 69, 211, 227, 277
TaaI ACNGT 2 cut(s) 181, 475
TaiI ACGT 2 cut(s) 63, 446
TaqI TCGA 3 cut(s) 54, 297, 670
TauI GCSGC 1 cut(s) 271
TfiI GAWTC 1 cut(s) 605
TscAI CASTG 1 cut(s) 82
TseFI GTSAC 1 cut(s) 65
TseI GCWGC 3 cut(s) 224, 271, 482
Tsp45I GTSAC 1 cut(s) 65
TspDTI ATGAA 1 cut(s) 654
TspGWI ACGGA 2 cut(s) 321, 608
TspRI CASTG 1 cut(s) 82
XhoI CTCGAG 1 cut(s) 53
XspI CTAG 2 cut(s) 170, 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.