Rw0G001250

Lysine methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00042
OG0016856
Physical Location & Seq
Reverse (-)
103974 .. 105045
1072 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G001250.1

Sequence Viewer

Length: 759 bp
ATGGATATAAAAAGCACCTACCATATAGCTAGTTCAAGTAAAGGAGCCGAGGAACTAATAGCCTCAGAGTCAATGGCCATGAGACAAATCCAAGTCGCCGACGACACGCTTGTAATCCATGAGCTCGAGGACGTTTGTGACCCGGCCACTGGCCGAGCACTCACCGGCTCATGGATTTGGGCCTCGGCTTTGGTCCTATCCAACTGGATGACCAACCATAGCCGAACCAACTTCAGTTTTCTAGGCAAGACCGTGCTTGAGCTTGGTGCAGGAGCTGGACTCCCCGGCTTGACCGCAGCCCGGCTAGGAGCGAGCCAAGTTGTGCTCACCGACATCAAGCCGCTGCTTCCAGGTTTGCTGAAGAATGTCGAAGCCAACGGACTTGGTGAACAAGTCAAGGTCAGGGAACTCATGTGGGGATCAGACGAGTCATTGAGCGAAGTCGGTGAGTTTGACTTGGTTTTGATGTCAGATGTGTTCTACAACGCCGAGGAGATGGTTGGCTTGGCCAAGACGTTGAAATGTGTGTGCAACAGAAAGACGACAGTTTTGGCAGCAACTGAGGTAAGGCCCTCAACTGCTGAGTGCCTGAGCGAGTTTGTTAGAGAAGGCTTTATGCTTGTTGAGTTGCCGAGTGAGTTCGCTTCTTCAGAAGGAATGGACACGGAGAGTCTGGATTCCTTTGCCATCTTCCAAGTCATACCACCGCATGAAGAAAGCCAAATGGATAGCCCTTGTAATCGAGACCACAGGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_16

Protein Analysis

252

Amino Acids

27.4

Weight (kDa)

4.58

Isoelectric Point (pI)

35.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_16 PF10294 55 - 192 2.1e-29 Lysine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016856)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g20871
malus_domestica MD06G1042700.v1.1
pyrus_communis pycom06g03560
rosa_chinensis RchiOBHm_Chr7g0207961
rosa_laevigata RLG00000003269
rosa_multiflora Rmu_sc0000905.1_g000011
rosa_roxburghii Rroxscaffold_3G00250980
rosa_rugosa Rorug07G0100500
rosa_samantha Rh7AG235800 Rh7BG229700 Rh7CG249300 Rh7DG240200
rosa_wichuraiana Rw0G001250 Rw0G017670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 294, 341, 707
AclWI GGATC 1 cut(s) 427
AcoI YGGCCR 4 cut(s) 75, 144, 151, 507
AcuI CTGAAG 3 cut(s) 217, 380, 633
AfiI CCNNNNNNNGG 3 cut(s) 149, 171, 300
AgsI TTSAA 2 cut(s) 36, 520
AjnI CCWGG 1 cut(s) 349
AluBI AGCT 4 cut(s) 29, 124, 262, 275
AluI AGCT 4 cut(s) 29, 124, 262, 275
Alw21I GWGCWC 3 cut(s) 126, 160, 327
Alw26I GTCTC 2 cut(s) 76, 738
AlwI GGATC 1 cut(s) 427
AlwNI CAGNNNCTG 2 cut(s) 275, 560
Ama87I CYCGRG 1 cut(s) 125
AoxI GGCC 6 cut(s) 75, 144, 151, 180, 507, 569
ApeKI GCWGC 3 cut(s) 296, 343, 554
ArsI GACNNNNNNTTYG 2 cut(s) 532, 564
AspS9I GGNCC 3 cut(s) 180, 193, 570
AsuC2I CCSGG 3 cut(s) 143, 285, 301
AsuHPI GGTGA 4 cut(s) 154, 319, 398, 458
AvaI CYCGRG 1 cut(s) 125
AvaII GGWCC 1 cut(s) 193
BalI TGGCCA 2 cut(s) 77, 509
BanII GRGCYC 1 cut(s) 126
Bbv12I GWGCWC 3 cut(s) 126, 160, 327
BbvI GCAGC 3 cut(s) 308, 330, 566
BccI CCATC 2 cut(s) 490, 695
BciT130I CCWGG 1 cut(s) 351
BcnI CCSGG 3 cut(s) 143, 285, 301
BcoDI GTCTC 2 cut(s) 76, 738
BfaI CTAG 3 cut(s) 30, 242, 305
BisI GCNGC 4 cut(s) 297, 341, 344, 555
BlsI GCNGC 4 cut(s) 298, 342, 345, 556
Bme1390I CCNGG 4 cut(s) 143, 285, 301, 351
Bme18I GGWCC 1 cut(s) 193
BmeT110I CYCGRG 1 cut(s) 125
BmgT120I GGNCC 3 cut(s) 180, 193, 570
BmiI GGNNCC 1 cut(s) 46
BmrFI CCNGG 4 cut(s) 143, 285, 301, 351
BplI GAGNNNNNCTC 2 cut(s) 264, 296
Bpu10I CCTNAGC 1 cut(s) 590
BpuEI CTTGAG 1 cut(s) 278
BpuMI CCSGG 3 cut(s) 143, 285, 301
BsaI GGTCTC 1 cut(s) 738
BsaJI CCNNGG 4 cut(s) 48, 183, 283, 489
Bsc4I CCNNNNNNNGG 3 cut(s) 149, 171, 300
Bse118I RCCGGY 1 cut(s) 164
Bse1I ACTGG 2 cut(s) 154, 209
BseBI CCWGG 1 cut(s) 351
BseDI CCNNGG 4 cut(s) 48, 183, 283, 489
BseGI GGATG 1 cut(s) 213
BseLI CCNNNNNNNGG 3 cut(s) 149, 171, 300
BseMII CTCAG 4 cut(s) 78, 552, 573, 581
BseNI ACTGG 2 cut(s) 154, 209
BseRI GAGGAG 1 cut(s) 506
BseXI GCAGC 3 cut(s) 308, 330, 566
BsgI GTGCAG 1 cut(s) 288
BshFI GGCC 6 cut(s) 77, 146, 153, 182, 509, 571
BsiHKAI GWGCWC 3 cut(s) 126, 160, 327
BsiHKCI CYCGRG 1 cut(s) 125
BsiSI CCGG 4 cut(s) 143, 165, 285, 301
BslI CCNNNNNNNGG 3 cut(s) 149, 171, 300
BsmAI GTCTC 2 cut(s) 76, 738
BsnI GGCC 6 cut(s) 77, 146, 153, 182, 509, 571
Bso31I GGTCTC 1 cut(s) 738
BsoBI CYCGRG 1 cut(s) 125
Bsp1286I GDGCHC 3 cut(s) 126, 160, 327
Bsp143I GATC 1 cut(s) 419
BspACI CCGC 3 cut(s) 294, 341, 707
BspANI GGCC 6 cut(s) 77, 146, 153, 182, 509, 571
BspCNI CTCAG 4 cut(s) 77, 553, 574, 582
BspLI GGNNCC 1 cut(s) 46
BspPI GGATC 1 cut(s) 427
BspTNI GGTCTC 1 cut(s) 738
BsrFI RCCGGY 1 cut(s) 164
BsrI ACTGG 2 cut(s) 154, 209
BssAI RCCGGY 1 cut(s) 164
BssECI CCNNGG 4 cut(s) 48, 183, 283, 489
BssMI GATC 1 cut(s) 419
Bst2UI CCWGG 1 cut(s) 351
Bst4CI ACNGT 2 cut(s) 253, 547
BstC8I GCNNGC 1 cut(s) 313
BstDEI CTNAG 4 cut(s) 64, 561, 582, 590
BstF5I GGATG 1 cut(s) 213
BstKTI GATC 1 cut(s) 422
BstMAI GTCTC 2 cut(s) 76, 738
BstMBI GATC 1 cut(s) 419
BstNI CCWGG 1 cut(s) 351
BstSCI CCNGG 4 cut(s) 141, 283, 299, 349
BstV1I GCAGC 3 cut(s) 308, 330, 566
BsuRI GGCC 6 cut(s) 77, 146, 153, 182, 509, 571
BtsCI GGATG 1 cut(s) 213
BtsIMutI CAGTG 1 cut(s) 147
Cac8I GCNNGC 1 cut(s) 313
CaiI CAGNNNCTG 2 cut(s) 275, 560
Cfr10I RCCGGY 1 cut(s) 164
Cfr13I GGNCC 3 cut(s) 180, 193, 570
CviAII CATG 6 cut(s) 79, 119, 171, 412, 710, 756
DdeI CTNAG 4 cut(s) 64, 561, 582, 590
DpnI GATC 1 cut(s) 421
DpnII GATC 1 cut(s) 419
EaeI YGGCCR 4 cut(s) 75, 144, 151, 507
Ecl136II GAGCTC 1 cut(s) 124
Eco24I GRGCYC 1 cut(s) 126
Eco31I GGTCTC 1 cut(s) 738
Eco47I GGWCC 1 cut(s) 193
Eco53kI GAGCTC 1 cut(s) 124
Eco57I CTGAAG 3 cut(s) 217, 380, 633
Eco88I CYCGRG 1 cut(s) 125
EcoICRI GAGCTC 1 cut(s) 124
EcoO109I RGGNCCY 1 cut(s) 570
EcoRII CCWGG 1 cut(s) 349
EcoT38I GRGCYC 1 cut(s) 126
FaeI CATG 6 cut(s) 82, 122, 174, 415, 713, 759
FatI CATG 6 cut(s) 78, 118, 170, 411, 709, 755
Fnu4HI GCNGC 4 cut(s) 297, 341, 344, 555
FokI GGATG 1 cut(s) 220
FriOI GRGCYC 1 cut(s) 126
Fsp4HI GCNGC 4 cut(s) 297, 341, 344, 555
FspBI CTAG 3 cut(s) 30, 242, 305
GluI GCNGC 4 cut(s) 297, 341, 344, 555
HaeIII GGCC 6 cut(s) 77, 146, 153, 182, 509, 571
HapII CCGG 4 cut(s) 143, 165, 285, 301
Hin1II CATG 6 cut(s) 82, 122, 174, 415, 713, 759
HinfI GANTC 5 cut(s) 68, 279, 428, 670, 677
HpaII CCGG 4 cut(s) 143, 165, 285, 301
HphI GGTGA 4 cut(s) 154, 319, 398, 458
Hpy166II GTNNAC 1 cut(s) 389
Hpy188I TCNGA 4 cut(s) 67, 424, 472, 652
Hpy188III TCNNGA 2 cut(s) 674, 743
Hpy8I GTNNAC 1 cut(s) 389
Hpy99I CGWCG 1 cut(s) 104
HpyAV CCTTC 2 cut(s) 602, 647
HpyCH4III ACNGT 2 cut(s) 253, 547
HpyCH4IV ACGT 2 cut(s) 132, 515
HpyCH4V TGCA 2 cut(s) 269, 531
HpyF3I CTNAG 4 cut(s) 64, 561, 582, 590
HpySE526I ACGT 2 cut(s) 132, 515
Hsp92II CATG 6 cut(s) 82, 122, 174, 415, 713, 759
Kzo9I GATC 1 cut(s) 419
LmnI GCTCC 3 cut(s) 44, 272, 308
Lsp1109I GCAGC 3 cut(s) 308, 330, 566
MaeI CTAG 3 cut(s) 30, 242, 305
MaeII ACGT 2 cut(s) 132, 515
MaeIII GTNAC 1 cut(s) 137
MalI GATC 1 cut(s) 421
MboI GATC 1 cut(s) 419
MboII GAAGA 4 cut(s) 373, 639, 682, 725
MhlI GDGCHC 3 cut(s) 126, 160, 327
MlsI TGGCCA 2 cut(s) 77, 509
MluNI TGGCCA 2 cut(s) 77, 509
MlyI GAGTC 4 cut(s) 77, 273, 437, 679
MmeI TCCRAC 1 cut(s) 225
MnlI CCTC 7 cut(s) 43, 73, 121, 193, 484, 556, 583
Mox20I TGGCCA 2 cut(s) 77, 509
MscI TGGCCA 2 cut(s) 77, 509
Msp20I TGGCCA 2 cut(s) 77, 509
MspA1I CMGCKG 1 cut(s) 343
MspI CCGG 4 cut(s) 143, 165, 285, 301
MspR9I CCNGG 4 cut(s) 143, 285, 301, 351
MvaI CCWGG 1 cut(s) 351
NciI CCSGG 3 cut(s) 143, 285, 301
NdeII GATC 1 cut(s) 419
NlaIII CATG 6 cut(s) 82, 122, 174, 415, 713, 759
NlaIV GGNNCC 1 cut(s) 46
NmeAIII GCCGAG 5 cut(s) 73, 164, 179, 514, 657
NmuCI GTSAC 1 cut(s) 137
PaeR7I CTCGAG 1 cut(s) 125
PfeI GAWTC 1 cut(s) 677
PkrI GCNGC 4 cut(s) 298, 342, 345, 556
PleI GAGTC 4 cut(s) 76, 273, 436, 678
PpsI GAGTC 4 cut(s) 76, 273, 436, 678
Psp124BI GAGCTC 1 cut(s) 126
Psp6I CCWGG 1 cut(s) 349
PspGI CCWGG 1 cut(s) 349
PspN4I GGNNCC 1 cut(s) 46
PspPI GGNCC 3 cut(s) 180, 193, 570
PspXI VCTCGAGB 1 cut(s) 125
PstNI CAGNNNCTG 2 cut(s) 275, 560
SacI GAGCTC 1 cut(s) 126
SatI GCNGC 4 cut(s) 297, 341, 344, 555
Sau3AI GATC 1 cut(s) 419
Sau96I GGNCC 3 cut(s) 180, 193, 570
SchI GAGTC 4 cut(s) 77, 273, 437, 679
ScrFI CCNGG 4 cut(s) 143, 285, 301, 351
SduI GDGCHC 3 cut(s) 126, 160, 327
Sfr274I CTCGAG 1 cut(s) 125
SinI GGWCC 1 cut(s) 193
SlaI CTCGAG 1 cut(s) 125
SmlI CTYRAG 2 cut(s) 125, 257
SmoI CTYRAG 2 cut(s) 125, 257
SsiI CCGC 3 cut(s) 294, 341, 707
SspMI CTAG 3 cut(s) 30, 242, 305
SstI GAGCTC 1 cut(s) 126
StyD4I CCNGG 4 cut(s) 141, 283, 299, 349
TaaI ACNGT 2 cut(s) 253, 547
TaiI ACGT 2 cut(s) 135, 518
TaqI TCGA 3 cut(s) 126, 369, 742
TauI GCSGC 1 cut(s) 343
TfiI GAWTC 1 cut(s) 677
TscAI CASTG 1 cut(s) 154
TseFI GTSAC 1 cut(s) 137
TseI GCWGC 3 cut(s) 296, 343, 554
Tsp45I GTSAC 1 cut(s) 137
TspDTI ATGAA 1 cut(s) 726
TspGWI ACGGA 2 cut(s) 393, 680
TspRI CASTG 1 cut(s) 154
VpaK11BI GGWCC 1 cut(s) 193
XhoI CTCGAG 1 cut(s) 125
XspI CTAG 3 cut(s) 30, 242, 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.