MD06G1078600.v1.1

N-terminal C2 in EEIG1 and EHBP1 proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0007404
Physical Location & Seq
Forward (+)
19405417 .. 19410725
5309 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1078600.v1.1.491

Sequence Viewer

Length: 3333 bp
ATGTTCAAGTCGGCGAGATGGAGAACCGACAAGAGCAAGATCAAAGAAGTCTTCAAACTGCAGTTCCGTGCAACTCAGGTGACACAGTTGGGTGTGGATGCATTGCTGGTATCTGTGGTTCCTGTGGATGTGGGGAAGCCAACTGTGAAATCAGATAAAGCTACAGTTCGTGACGGGAACTGTCGATGGGAGAATCCGGTCTATGAAACAGTTAAATTCGTCCGCGAGCCAAGAACCGGGAAGATCAAAGAGAGTTTCTACAACTTTGTTGTCTCAGCTGGATCGTCCAAGGCTAGTGTTCTTGGGGAGGTTTCTGTCGATTTTGCTGACTATGCCGAGGCTACTAAATCGTCCTGTGTATCTCTTCCCCTCAAGAATTCAAATTCTAATGCAATTATGCATGTTACCATTCAAAGGTTGCAAGCAAATGTTGATCTGAGAGAGGTAGAAGGCGGCGAAGATGCATATGCTAAATCCCAGGATAGAAGCTTCAAGAACCTCTTAAGCAATAAGGATACGGATGAAAGCGTTTCGATTGATGAGGGGATTAATAGAACCACCCAAATTGCAGAGCTCAACCGCAGAGCATCTATTGGATCTGACATAACGTTGTCAAGTTCTGATAGCGGCTCTGGACTTGATACTCCGCGAGAACTTGGATCAAGAGATCCTTCCAACTTTCTGTTGTCTTTGAGTCACACCTCAGTACAACATCAACCTGCTGTTTATACCCGAACAACAACCTATGACGACAATCAGCGATCACAATGGGCGTGGTCTGCAGGTTCAGAACATGGAGTAAGTACTGATGACTCAACAAAGAGTTCTCATGACACCCTTGCCAGAGAACGGTCTCAACAGTCTTCAGATGACGATATTGAAAAGCTAAAGGCTGATCTTGTTGTTCTAGCTAGACAGGCAGATATGTCAGAGCTAGAATTACAAACATTAAGGAAGCAGATAGTAAAGGAGAGCAAAAGGGGGCAGGATCTTTCAAGAGAAGTCATTAGCTTGAAAGAGGAACGAGATTCTTTCAAGGCGGAATGTGAGAAACTAAAGTCTTTTCAGAAACGTGTGGATGATGCAAAAATTAGAAACAGGTTCCAGTTAGAGGGCGGGGATCTGCGGGCTCTTGTTGATGAAATTAGACAAGAATTGAGTTATGAGAAGGACCTGAGTTTCAATCTCCGTTTACAGCTCCAGAAGACGCAAGAATCAAATTCTGATTTAATTCTTGCAGTACGAGACCTGGAAGAAATTTTGGAGCAGAAAAATAGTGAAATAGCCAATCTTTCCAACAGGCCAGAATCCTGCGGGGATGCTGCCGGGTTGAAGGCAACCATCTCTAAAGGTGGGACAAGTGAGGATGAGGAGCAATTAGAGCTGGAGGATCTTGTTCAGGAGCACAGCAATGCCAAGAAAACACACATGCTCGAGAAACAGATTGCCGAACTCTATGGTGAAATAGAAATCTATAGGAGAGACAAAGATGAGCTCGAGATGCAAATGGAGCAGCTTGCACTGGACTATGAGATACTGAAACAGGAAAACCATGACATCTCATATAAACTAGAGCAAAGCCAACTGCAAGAACAACTTAAGATGCAGTACGAATGCTCGTCTCCCTCTGCTAGCATAAATGAACTCGAATGCCAAGTTGAGGATTTGGAAACAGAACTGAAGAGGCAGTCTGAAGATTTTTCAAATTCGTTGGCTACCATAAAGGAACTTGAGTCCCATATCAAAAGCTTGGAGGCAGAACTGGAAAAGCAGGCACAAGTGTCTAAAACTGATTTAGAGGCTGTGACATGTGCCAGAGTTGAGGATTTGGAAACGGAACTGAAGAAGCAGTCAGAAGAGTATTCGAATACGTTGGCTACCATAAAGGAACTTGAATTCCATATCAGAAGCTTGGAGGAAGAACTGGAGAAGCAGGCACAAATATTTGAAGCTGATTTAGAGGCTGTAACATGTGCCAAAGTTGAGCAGGAGCAAAGAGCCATCCGAGCGGAGGAGGCACTGCGAAAAACTAGATCAAAAAATGCTAATACAGCTGAGAGGCTTCAGGAAGAATTTCGAAGACTCTCTGTGCAGATGGCCTCAACGTTTGATGCAAATGAAAAGGTAGCTTTGAAAGCAATGACAGAAGCCAGTGAATTGTGTGCGCAGAAGAGTCAACTAGAAGAAATGCTCCAAAAAACGAAGGACGAGCTCCAGGAAGTTAGAAATGATTACGAGACACGGTTGCAGAAGCTCTCTTGCCACTTAGACGAGAAAACCAGACAGATCGAAGAGATTGTATTGGAAATGGACAACAATTCCAAGCAGCTCGACCATCAGCAGAAACAAGAGGAAGAAGTAAAGGGAAATCTCTCCCAGGTTATCTTACAGCTCAAATCTCAGATTGAAAGGCTTACGGCAGAGAATAACAGCCTTTCTGAGCAAGTGGAGGAAAGCAAAAATTTGAGAGCTGAGTTGGAACAAATGAAGAAATCAATCGAGGAAGCTGAAATGCGGATACAAAGAGGAAATGCGGAAAGAAATGAGCTGGTAGGTACAATTTCTATTCTTAAGAAGGAAGCAGAGAAGTCATTGGAGGGTTTGAATAGAATGCAGCATCTCAAAGATGAAAAGGAGGTAACAATTGGACTCCTAAAATCAGAGACAGAAGAGCTTAAGGCTCGGTGTAAGGACTTTAAACATGCTATATATGAGGATGAGGTAGAGAAAGAGAAACTTAGAAAGCAGGTATTTCAGCTTAAGGCTGACCTGAAAAAGAAGGAAGATGCGTTCACTACTGTTGAAAAGAAGCTCAAGGAAAGCAATGGGCGCGCAATAGTTTCTGATGGAATTAAAACTACTCAGAGAAACAATAAGTCTTCGCCAGTTGCTCGTGGTTCAAAAGAAGTTGCAAGTATGAAAGAGAGAATAAAATTGCTTGAGGGACAAATAAAATCAAAGGAAGCTGCTTTGGAAACCTCAACTGCTGCATTTTTGGAGAAGGAAAAGGATCTTCAGAACGTAATCGAAGAATTAGAGAGCAGGGTGGAAGAAATAAATCAGAATAGTGCATTTCAAATGGTAGCTAAAGATGCCACTAGCGATGAGATTGCATCGGAACAAGAACCAAGTTTTTCCACTGCTGATAGCAGGTACGGCGACTTATTAACTGAATTAGCATCACTGAAGGAGAAGAATATATCAATGGAAAGCGAACTAAAAGATATGCAAGAAAGATATTCAGAGATAAGTCTCAAATTTGCAGTGGTTGAAGGTGAAAGACAACAGCTCGTAATGACAGTACGCAACCTCAAAAATTCCAAGAGGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04530 map04530
Pfam Domains
Protein Families
NT-C2

Protein Analysis

1111

Amino Acids

126.36

Weight (kDa)

5.05

Isoelectric Point (pI)

48.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 11 - 144 1.5e-21 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2166
Acc36I ACCTGC 4 cut(s) 727, 773, 2737, 3141
AccBSI CCGCTC 1 cut(s) 2009
AccII CGCG 3 cut(s) 225, 649, 2832
AclI AACGTT 2 cut(s) 608, 2105
AclWI GGATC 8 cut(s) 289, 604, 662, 667, 996, 1128, 1398, 3018
AcuI CTGAAG 7 cut(s) 849, 1700, 1713, 1862, 2048, 2999, 3206
AfaI GTAC 7 cut(s) 708, 805, 1242, 1610, 2557, 3155, 3303
AfiI CCNNNNNNNGG 6 cut(s) 236, 414, 849, 1111, 1660, 2011
AflII CTTAAG 5 cut(s) 502, 1598, 2570, 2675, 2759
AflIII ACRYGT 3 cut(s) 1072, 1808, 1970
AjnI CCWGG 4 cut(s) 477, 1248, 2214, 2376
AloI GAACNNNNNNTCC 4 cut(s) 1881, 1913, 2774, 2806
Alw21I GWGCWC 4 cut(s) 576, 1407, 1497, 2214
Alw26I GTCTC 8 cut(s) 277, 858, 1239, 1476, 1626, 2231, 2657, 3257
AlwI GGATC 8 cut(s) 289, 604, 662, 667, 996, 1128, 1398, 3018
Ama87I CYCGRG 2 cut(s) 1433, 1496
AoxI GGCC 2 cut(s) 1301, 2097
ApeKI GCWGC 6 cut(s) 1322, 1513, 2326, 2614, 2966, 2987
AseI ATTAAT 1 cut(s) 549
Asp700I GAANNNNTTC 1 cut(s) 2073
AspLEI GCGC 3 cut(s) 2167, 2832, 2834
AspS9I GGNCC 1 cut(s) 1171
AsuC2I CCSGG 2 cut(s) 238, 1327
AsuHPI GGTGA 3 cut(s) 91, 1472, 3287
AsuII TTCGAA 2 cut(s) 1865, 2077
AsuNHI GCTAGC 1 cut(s) 1631
AvaI CYCGRG 2 cut(s) 1433, 1496
AvaII GGWCC 1 cut(s) 1171
BanII GRGCYC 4 cut(s) 576, 1132, 1497, 2214
BauI CACGAG 1 cut(s) 2892
BbsI GAAGAC 5 cut(s) 43, 855, 1211, 2086, 2871
Bbv12I GWGCWC 4 cut(s) 576, 1407, 1497, 2214
BbvI GCAGC 6 cut(s) 1309, 1525, 2338, 2626, 2953, 2974
BccI CCATC 7 cut(s) 12, 180, 1349, 2009, 2089, 2343, 2840
BceAI ACGGC 2 cut(s) 2433, 3172
BcgI CGANNNNNNTGC 2 cut(s) 3092, 3126
BciT130I CCWGG 4 cut(s) 479, 1250, 2216, 2378
BciVI GTATCC 2 cut(s) 508, 2511
BcnI CCSGG 2 cut(s) 238, 1327
BcoDI GTCTC 8 cut(s) 277, 858, 1239, 1476, 1626, 2231, 2657, 3257
BfaI CTAG 9 cut(s) 294, 908, 912, 935, 1571, 1632, 2031, 2180, 3099
BfmI CTRYAG 4 cut(s) 59, 162, 780, 1474
BfrI CTTAAG 5 cut(s) 502, 1598, 2570, 2675, 2759
BfuAI ACCTGC 4 cut(s) 727, 773, 2737, 3141
BfuI GTATCC 2 cut(s) 508, 2511
BisI GCNGC 8 cut(s) 454, 628, 1323, 1514, 2327, 2615, 2967, 2988
BlsI GCNGC 8 cut(s) 455, 629, 1324, 1515, 2328, 2616, 2968, 2989
BmcAI AGTACT 1 cut(s) 805
Bme1390I CCNGG 6 cut(s) 238, 479, 1250, 1327, 2216, 2378
Bme18I GGWCC 1 cut(s) 1171
BmeT110I CYCGRG 2 cut(s) 1433, 1496
BmgT120I GGNCC 1 cut(s) 1171
BmiI GGNNCC 2 cut(s) 120, 1103
BmrFI CCNGG 6 cut(s) 238, 479, 1250, 1327, 2216, 2378
BmtI GCTAGC 1 cut(s) 1635
BpiI GAAGAC 5 cut(s) 43, 855, 1211, 2086, 2871
BpmI CTGGAG 4 cut(s) 1184, 1406, 1946, 2198
Bpu14I TTCGAA 2 cut(s) 1865, 2077
BpuEI CTTGAG 4 cut(s) 356, 1751, 2798, 2960
BpuMI CCSGG 2 cut(s) 238, 1327
BsaI GGTCTC 2 cut(s) 858, 1239
BsaJI CCNNGG 4 cut(s) 288, 336, 477, 2376
BsaWI WCCGGW 1 cut(s) 196
Bsc4I CCNNNNNNNGG 6 cut(s) 236, 414, 849, 1111, 1660, 2011
Bse1I ACTGG 6 cut(s) 1105, 1527, 1767, 1929, 2151, 2885
Bse3DI GCAATG 4 cut(s) 101, 1417, 2145, 2830
BseBI CCWGG 4 cut(s) 479, 1250, 2216, 2378
BseDI CCNNGG 4 cut(s) 288, 336, 477, 2376
BseGI GGATG 8 cut(s) 103, 133, 526, 1084, 1324, 1372, 2001, 2722
BseLI CCNNNNNNNGG 6 cut(s) 236, 414, 849, 1111, 1660, 2011
BseMI GCAATG 4 cut(s) 101, 1417, 2145, 2830
BseNI ACTGG 6 cut(s) 1105, 1527, 1767, 1929, 2151, 2885
BsePI GCGCGC 1 cut(s) 2830
BseRI GAGGAG 2 cut(s) 1385, 2027
BseXI GCAGC 6 cut(s) 1309, 1525, 2338, 2626, 2953, 2974
BsgI GTGCAG 1 cut(s) 2111
Bsh1236I CGCG 3 cut(s) 225, 649, 2832
BshFI GGCC 2 cut(s) 1303, 2099
BsiHKAI GWGCWC 4 cut(s) 576, 1407, 1497, 2214
BsiHKCI CYCGRG 2 cut(s) 1433, 1496
BsiSI CCGG 3 cut(s) 197, 237, 1326
BslFI GGGAC 3 cut(s) 1369, 1720, 2958
BslI CCNNNNNNNGG 6 cut(s) 236, 414, 849, 1111, 1660, 2011
BsmAI GTCTC 8 cut(s) 277, 858, 1239, 1476, 1626, 2231, 2657, 3257
BsmBI CGTCTC 1 cut(s) 1626
BsmFI GGGAC 3 cut(s) 1369, 1720, 2958
BsmI GAATGC 3 cut(s) 1619, 1655, 2616
BsnI GGCC 2 cut(s) 1303, 2099
Bso31I GGTCTC 2 cut(s) 858, 1239
BsoBI CYCGRG 2 cut(s) 1433, 1496
Bsp119I TTCGAA 2 cut(s) 1865, 2077
Bsp1286I GDGCHC 5 cut(s) 576, 1132, 1407, 1497, 2214
BspANI GGCC 2 cut(s) 1303, 2099
BspFNI CGCG 3 cut(s) 225, 649, 2832
BspHI TCATGA 1 cut(s) 829
BspLI GGNNCC 2 cut(s) 120, 1103
BspMAI CTGCAG 2 cut(s) 63, 784
BspMI ACCTGC 4 cut(s) 727, 773, 2737, 3141
BspOI GCTAGC 1 cut(s) 1635
BspPI GGATC 8 cut(s) 289, 604, 662, 667, 996, 1128, 1398, 3018
BspQI GCTCTTC 1 cut(s) 2664
BspT104I TTCGAA 2 cut(s) 1865, 2077
BspTI CTTAAG 5 cut(s) 502, 1598, 2570, 2675, 2759
BspTNI GGTCTC 2 cut(s) 858, 1239
BsrBI CCGCTC 1 cut(s) 2009
BsrDI GCAATG 4 cut(s) 101, 1417, 2145, 2830
BsrI ACTGG 6 cut(s) 1105, 1527, 1767, 1929, 2151, 2885
BssECI CCNNGG 4 cut(s) 288, 336, 477, 2376
BssHII GCGCGC 1 cut(s) 2830
BssSI CACGAG 1 cut(s) 2892
BssT1I CCWWGG 1 cut(s) 288
Bst2BI CACGAG 1 cut(s) 2892
Bst2UI CCWGG 4 cut(s) 479, 1250, 2216, 2378
Bst6I CTCTTC 6 cut(s) 369, 1676, 1851, 2165, 2286, 2664
BstAFI CTTAAG 5 cut(s) 502, 1598, 2570, 2675, 2759
BstBI TTCGAA 2 cut(s) 1865, 2077
BstC8I GCNNGC 8 cut(s) 227, 423, 1128, 1518, 1633, 1773, 1935, 2832
BstF5I GGATG 8 cut(s) 103, 133, 526, 1084, 1324, 1372, 2001, 2722
BstFNI CGCG 3 cut(s) 225, 649, 2832
BstHHI GCGC 3 cut(s) 2167, 2832, 2834
BstMAI GTCTC 8 cut(s) 277, 858, 1239, 1476, 1626, 2231, 2657, 3257
BstNI CCWGG 4 cut(s) 479, 1250, 2216, 2378
BstNSI RCATGY 5 cut(s) 404, 1432, 1812, 1974, 2705
BstSCI CCNGG 6 cut(s) 236, 477, 1248, 1325, 2214, 2376
BstSFI CTRYAG 4 cut(s) 59, 162, 780, 1474
BstUI CGCG 3 cut(s) 225, 649, 2832
BstV1I GCAGC 6 cut(s) 1309, 1525, 2338, 2626, 2953, 2974
BstV2I GAAGAC 5 cut(s) 43, 855, 1211, 2086, 2871
BstX2I RGATCY 6 cut(s) 596, 667, 988, 1120, 1390, 3010
BstYI RGATCY 6 cut(s) 596, 667, 988, 1120, 1390, 3010
BsuI GTATCC 2 cut(s) 508, 2511
BsuRI GGCC 2 cut(s) 1303, 2099
BtgZI GCGATG 1 cut(s) 3117
BtsCI GGATG 8 cut(s) 103, 133, 526, 1084, 1324, 1372, 2001, 2722
BtsI GCAGTG 3 cut(s) 2018, 3138, 3270
BtsIMutI CAGTG 6 cut(s) 1520, 2018, 2158, 3138, 3182, 3270
BveI ACCTGC 4 cut(s) 727, 773, 2737, 3141
Cac8I GCNNGC 8 cut(s) 227, 423, 1128, 1518, 1633, 1773, 1935, 2832
CciI TCATGA 1 cut(s) 829
CfoI GCGC 3 cut(s) 2167, 2832, 2834
Cfr13I GGNCC 1 cut(s) 1171
CseI GACGC 1 cut(s) 1216
Csp6I GTAC 7 cut(s) 707, 804, 1241, 1609, 2556, 3154, 3302
CspCI CAANNNNNGTGG 4 cut(s) 547, 582, 755, 790
CviAII CATG 8 cut(s) 401, 794, 830, 1429, 1553, 1809, 1971, 2702
CviQI GTAC 7 cut(s) 707, 804, 1241, 1609, 2556, 3154, 3302
DraI TTTAAA 1 cut(s) 2698
Eam1104I CTCTTC 6 cut(s) 369, 1676, 1851, 2165, 2286, 2664
EarI CTCTTC 6 cut(s) 369, 1676, 1851, 2165, 2286, 2664
EciI GGCGGA 1 cut(s) 1055
Ecl136II GAGCTC 3 cut(s) 574, 1495, 2212
Eco130I CCWWGG 1 cut(s) 288
Eco24I GRGCYC 4 cut(s) 576, 1132, 1497, 2214
Eco31I GGTCTC 2 cut(s) 858, 1239
Eco47I GGWCC 1 cut(s) 1171
Eco53kI GAGCTC 3 cut(s) 574, 1495, 2212
Eco57I CTGAAG 7 cut(s) 849, 1700, 1713, 1862, 2048, 2999, 3206
Eco88I CYCGRG 2 cut(s) 1433, 1496
EcoICRI GAGCTC 3 cut(s) 574, 1495, 2212
EcoO109I RGGNCCY 1 cut(s) 1171
EcoRI GAATTC 2 cut(s) 376, 1895
EcoRII CCWGG 4 cut(s) 477, 1248, 2214, 2376
EcoT14I CCWWGG 1 cut(s) 288
EcoT22I ATGCAT 3 cut(s) 103, 402, 466
EcoT38I GRGCYC 4 cut(s) 576, 1132, 1497, 2214
ErhI CCWWGG 1 cut(s) 288
Esp3I CGTCTC 1 cut(s) 1626
FaeI CATG 8 cut(s) 404, 797, 833, 1432, 1556, 1812, 1974, 2705
FalI AAGNNNNNCTT 8 cut(s) 485, 517, 655, 687, 1581, 1613, 2721, 2753
FaqI GGGAC 3 cut(s) 1369, 1720, 2958
FatI CATG 8 cut(s) 400, 793, 829, 1428, 1552, 1808, 1970, 2701
FauI CCCGC 3 cut(s) 1109, 1119, 1307
FauNDI CATATG 1 cut(s) 466
Fnu4HI GCNGC 8 cut(s) 454, 628, 1323, 1514, 2327, 2615, 2967, 2988
FokI GGATG 8 cut(s) 110, 140, 533, 1091, 1331, 1379, 1988, 2729
FriOI GRGCYC 4 cut(s) 576, 1132, 1497, 2214
Fsp4HI GCNGC 8 cut(s) 454, 628, 1323, 1514, 2327, 2615, 2967, 2988
FspBI CTAG 9 cut(s) 294, 908, 912, 935, 1571, 1632, 2031, 2180, 3099
FspI TGCGCA 1 cut(s) 2166
GlaI GCGC 3 cut(s) 2166, 2831, 2833
GluI GCNGC 8 cut(s) 454, 628, 1323, 1514, 2327, 2615, 2967, 2988
GsuI CTGGAG 4 cut(s) 1184, 1406, 1946, 2198
HaeIII GGCC 2 cut(s) 1303, 2099
HapII CCGG 3 cut(s) 197, 237, 1326
HgaI GACGC 1 cut(s) 1216
HhaI GCGC 3 cut(s) 2167, 2832, 2834
Hin1II CATG 8 cut(s) 404, 797, 833, 1432, 1556, 1812, 1974, 2705
Hin6I GCGC 3 cut(s) 2165, 2830, 2832
HinP1I GCGC 3 cut(s) 2165, 2830, 2832
HincII GTYRAC 1 cut(s) 2177
HindII GTYRAC 1 cut(s) 2177
HindIII AAGCTT 3 cut(s) 487, 1747, 1909
HpaII CCGG 3 cut(s) 197, 237, 1326
HphI GGTGA 3 cut(s) 91, 1472, 3287
Hpy166II GTNNAC 3 cut(s) 1193, 2177, 2793
Hpy8I GTNNAC 3 cut(s) 1193, 2177, 2793
HpyCH4IV ACGT 5 cut(s) 608, 1072, 1871, 2105, 3021
HpySE526I ACGT 5 cut(s) 608, 1072, 1871, 2105, 3021
Hsp92II CATG 8 cut(s) 404, 797, 833, 1432, 1556, 1812, 1974, 2705
HspAI GCGC 3 cut(s) 2165, 2830, 2832
LguI GCTCTTC 1 cut(s) 2664
LmnI GCTCC 9 cut(s) 1203, 1264, 1372, 1402, 1510, 1990, 2196, 2217, 3327
Lsp1109I GCAGC 6 cut(s) 1309, 1525, 2338, 2626, 2953, 2974
MaeI CTAG 9 cut(s) 294, 908, 912, 935, 1571, 1632, 2031, 2180, 3099
MaeII ACGT 5 cut(s) 608, 1072, 1871, 2105, 3021
MaeIII GTNAC 7 cut(s) 79, 170, 403, 695, 1804, 1966, 2638
MbiI CCGCTC 1 cut(s) 2009
MfeI CAATTG 1 cut(s) 2643
MflI RGATCY 6 cut(s) 596, 667, 988, 1120, 1390, 3010
MhlI GDGCHC 5 cut(s) 576, 1132, 1407, 1497, 2214
MlyI GAGTC 6 cut(s) 703, 806, 1742, 2076, 2182, 2643
MmeI TCCRAC 3 cut(s) 699, 1320, 2457
Mph1103I ATGCAT 3 cut(s) 103, 402, 466
MroXI GAANNNNTTC 1 cut(s) 2073
MslI CAYNNNNRTG 1 cut(s) 1410
MspA1I CMGCKG 2 cut(s) 278, 2054
MspCI CTTAAG 5 cut(s) 502, 1598, 2570, 2675, 2759
MspI CCGG 3 cut(s) 197, 237, 1326
MspR9I CCNGG 6 cut(s) 238, 479, 1250, 1327, 2216, 2378
MunI CAATTG 1 cut(s) 2643
Mva1269I GAATGC 3 cut(s) 1619, 1655, 2616
MvaI CCWGG 4 cut(s) 479, 1250, 2216, 2378
MvnI CGCG 3 cut(s) 225, 649, 2832
NciI CCSGG 2 cut(s) 238, 1327
NdeI CATATG 1 cut(s) 466
NheI GCTAGC 1 cut(s) 1631
NlaIII CATG 8 cut(s) 404, 797, 833, 1432, 1556, 1812, 1974, 2705
NlaIV GGNNCC 2 cut(s) 120, 1103
NmeAIII GCCGAG 1 cut(s) 361
NmuCI GTSAC 4 cut(s) 79, 170, 695, 1804
NsbI TGCGCA 1 cut(s) 2166
NsiI ATGCAT 3 cut(s) 103, 402, 466
NspI RCATGY 5 cut(s) 404, 1432, 1812, 1974, 2705
NspV TTCGAA 2 cut(s) 1865, 2077
PaeR7I CTCGAG 2 cut(s) 1433, 1496
PagI TCATGA 1 cut(s) 829
PauI GCGCGC 1 cut(s) 2830
PciI ACATGT 2 cut(s) 1808, 1970
PciSI GCTCTTC 1 cut(s) 2664
PctI GAATGC 3 cut(s) 1619, 1655, 2616
PdmI GAANNNNTTC 1 cut(s) 2073
PfeI GAWTC 4 cut(s) 193, 1028, 1214, 1307
PfoI TCCNGGA 1 cut(s) 2214
PkrI GCNGC 8 cut(s) 455, 629, 1324, 1515, 2328, 2616, 2968, 2989
PleI GAGTC 6 cut(s) 702, 806, 1741, 2076, 2181, 2643
PpsI GAGTC 6 cut(s) 702, 806, 1741, 2076, 2181, 2643
PpuMI RGGWCCY 1 cut(s) 1171
PscI ACATGT 2 cut(s) 1808, 1970
PshBI ATTAAT 1 cut(s) 549
Psp124BI GAGCTC 3 cut(s) 576, 1497, 2214
Psp1406I AACGTT 2 cut(s) 608, 2105
Psp5II RGGWCCY 1 cut(s) 1171
Psp6I CCWGG 4 cut(s) 477, 1248, 2214, 2376
PspGI CCWGG 4 cut(s) 477, 1248, 2214, 2376
PspN4I GGNNCC 2 cut(s) 120, 1103
PspPI GGNCC 1 cut(s) 1171
PspPPI RGGWCCY 1 cut(s) 1171
PsrI GAACNNNNNNTAC 2 cut(s) 102, 134
PstI CTGCAG 2 cut(s) 63, 784
PsuI RGATCY 6 cut(s) 596, 667, 988, 1120, 1390, 3010
PteI GCGCGC 1 cut(s) 2830
PvuII CAGCTG 2 cut(s) 278, 2054
RsaI GTAC 7 cut(s) 708, 805, 1242, 1610, 2557, 3155, 3303
RsaNI GTAC 7 cut(s) 707, 804, 1241, 1609, 2556, 3154, 3302
RseI CAYNNNNRTG 1 cut(s) 1410
SacI GAGCTC 3 cut(s) 576, 1497, 2214
SapI GCTCTTC 1 cut(s) 2664
SatI GCNGC 8 cut(s) 454, 628, 1323, 1514, 2327, 2615, 2967, 2988
Sau96I GGNCC 1 cut(s) 1171
ScaI AGTACT 1 cut(s) 805
SchI GAGTC 6 cut(s) 703, 806, 1742, 2076, 2182, 2643
ScrFI CCNGG 6 cut(s) 238, 479, 1250, 1327, 2216, 2378
SduI GDGCHC 5 cut(s) 576, 1132, 1407, 1497, 2214
SfcI CTRYAG 4 cut(s) 59, 162, 780, 1474
Sfr274I CTCGAG 2 cut(s) 1433, 1496
SfuI TTCGAA 2 cut(s) 1865, 2077
SinI GGWCC 1 cut(s) 1171
SlaI CTCGAG 2 cut(s) 1433, 1496
SmiMI CAYNNNNRTG 1 cut(s) 1410
SspI AATATT 1 cut(s) 1944
SspMI CTAG 9 cut(s) 294, 908, 912, 935, 1571, 1632, 2031, 2180, 3099
SstI GAGCTC 3 cut(s) 576, 1497, 2214
StyD4I CCNGG 6 cut(s) 236, 477, 1248, 1325, 2214, 2376
StyI CCWWGG 1 cut(s) 288
TaiI ACGT 5 cut(s) 611, 1075, 1874, 2108, 3024
TatI WGTACW 2 cut(s) 706, 803
TauI GCSGC 2 cut(s) 456, 630
TfiI GAWTC 4 cut(s) 193, 1028, 1214, 1307
TscAI CASTG 6 cut(s) 1527, 2025, 2158, 3145, 3189, 3270
TseFI GTSAC 4 cut(s) 79, 170, 695, 1804
TseI GCWGC 6 cut(s) 1322, 1513, 2326, 2614, 2966, 2987
Tsp45I GTSAC 4 cut(s) 79, 170, 695, 1804
TspDTI ATGAA 8 cut(s) 219, 537, 1155, 1656, 2133, 2501, 2643, 2933
TspGWI ACGGA 4 cut(s) 56, 533, 1178, 1850
TspRI CASTG 6 cut(s) 1527, 2025, 2158, 3145, 3189, 3270
Vha464I CTTAAG 5 cut(s) 502, 1598, 2570, 2675, 2759
VpaK11BI GGWCC 1 cut(s) 1171
VspI ATTAAT 1 cut(s) 549
XceI RCATGY 5 cut(s) 404, 1432, 1812, 1974, 2705
XhoI CTCGAG 2 cut(s) 1433, 1496
XmnI GAANNNNTTC 1 cut(s) 2073
XspI CTAG 9 cut(s) 294, 908, 912, 935, 1571, 1632, 2031, 2180, 3099
ZrmI AGTACT 1 cut(s) 805
Zsp2I ATGCAT 3 cut(s) 103, 402, 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.