pycom06g07600

N-terminal C2 in EEIG1 and EHBP1 proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0007404
Physical Location & Seq
Forward (+)
12435004 .. 12439594
4591 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g07600.1

Sequence Viewer

Length: 3333 bp
ATGTTCAAGTCGGCGAGATGGAGAACCGACAAGAGCAGGATCAAAGAAGTCTTCAAACTGCAGTTCCGTGCAACTCAGGTGACACAGTTGGGTGTGGATGCATTGCTGGTATCTGTGGTTCCTGTGGATGTGGGGAAGCCAACTGTGAAATCAGATAAAGCTACAGTTCGTGACGGGAATTGTCGATGGGAGAATCCGGTCTATGAAACAGTTAAATTCGTCCGCGAGCCAAGAACCGGGAAGATCAAAGAGAGTGTCTACAACTTTGTTGTCTCAGCTGGACCGTCCAAGGCTAGTGTTCTTGGGGAGGTTTCTGTCGATTTTGCTGACTATGCCGAGGCTACTAAATCGTCCTGTGTATCTCTCCCCCTCAAGAATTCAAATTCTAATGCAATTATGCATGTTACCATTCAAAGGTTGCAAGCAAATGTTGATCCGAGAGAGGTAGAAGGCGGCGAAGATGCAAATGCTAAATCCCCGGATAGAAGCTTCAAGAACCTCTTAAGCAATAAGGATACGGATGAAAGCGTTTCGATTGATGAGGGGATTAATAGAACCACCCAAATTGCAGAGCTCAACCGCAGAGCATCCATTGGATCTGACATAACGTTGTCAAGTTCTGATAGCGGCTCTGGACTTGATACTCCGCGAGAACTTGGATCAAGAGATCCTTCCAACTTTTTGTCGTCTTTGAGTCACACCTCAGTACAACATCAACATGCTGTTTATACCCGAACAACAACCTATGATGACAATCAGCGATCACAATGGGCATGGTCTGCAGGTTCAGAACATGGAGTAAGTACTGATTGCTCAACAAAGAGTTCTCATGACACCCTTCCAAGAGAACGGTCTCAACAGTCTTCAGATGACGATATTGAAAAGCTAAAGGCTGATCTTGTTGTTCTAGCTAGACAGGCAGATATGTCAGAGCTAGAATTACAAACATTAAGGAAGCAGATAGTAAAGGAGAGCAAAAGGGGGCAGGATCTTTCAAGAGAAGTCATTAGCTTGAAAGAGGAACGAGATTCTTTCAAGGCGGAATGTGAGAAACTAAAGTCCTTTCAGAAACGTATGGATGATGCAAAAATTAGAAACAGGTTCCAGTTAGAAGGCGGGGATCTGCAGGCTCTTGTTGATGAAATTAGACAAGAATTGAGTTATGAAAAGGACCTGAGTTTCAATCTCCGTTTACAGCTCCAGAAGACGCAAGAATCAAATTCTGATTTAATTCTTGCAGTACGAGACCTGGAAGAAATTTTGGAGCAGAAAAATAGTGAAATAGCCAATCTTTCCAACAGGCCAGAATCCTGCAGGGAAGCTGCCGGGTTGAAGGCAACCATCTCTAAAGGTGGGACAAGTGAGGATGAGGTGCAATTAGAGCTGGAGGATCTTGTTCAGGAGCACAGCAATGCCAAGAAAACACACATGCTCGAGAAACAGATTGCCGAACTCTATGGTGAAATAGAAATCTATAGGAGAGACAAAGATGAGCTCGAGATGCAAATGGAGCAGCTTGCACTGGACTATGAGATACTGAAACAGGAAAACCATGACATCTCATATAAACTAGAGCAAAGCCAACTGCAAGAACAACTTAAGATGCAGTACGAATGCTCGTCTCCCTCTGCTAGCATAAATGAACTCGAATGCCAAGTTGAGGATTTGGAAACAGAACTGAAGAAGCAGTCTGAAGATTTTTCAAATTCGTTGGCTACCATAAAGGAACTTGAGTCCCATATCAAAAGCTTGGAGGCAGAACTGGAGAAGCAGGCACAAGTGTCTAAAACTGATTTAGAGGGTGTGACAAGTGCCAGAGTTGAGGATTTGGAAACGGAACTGAAAAAGCAGTCAGAAGAATTTTCGAATACGTTGGCTACCATAAAGGAACTTGAATTCCATATCAGAAGCTTGGAGGAAGAACTGGAGAAGCAGGCACAAATATTTGAAGCTGATTTAGAGGCTGTAACATGTGCCAAAGTTGAGCAGGAGCAAAGAGCCATCCGAGCGGAGGAGGCACTGCGAAAAACTAGATCAAAAAATGCTAATACAGCTGAGAGGCTTCAGGAAGAATTTCGAAGACTCTCAGTGCAGATGGCCTCAATGTTTGATGTAAATGAAAAGGTAGCTTTGAAAGCAATGGCAGAAGCCAGTGAATTGTGTGCGCAGAAGAGTCAACTAGAAGAAATGCTCCAAAAAACGAAGGACGAGCTCCAGGAAGTTAGAAATGATTACGAGACAAGGTTGCAGAAGCTCTCTTGCCACTTAGACGAGAAAACCAGACAGATCGAAGAGATTGTATTGGAAATGGAGGACAATACCAAGCAGCTCGGCCATCAGCAGAAACAAGAGGAAGAAGTAAAGGGAAATCTCTCCCAGGTTATCTTACAGCTCAAATCTCAGATTGAAAGGCTTACGGCAGAGAATAACAGCCTTTCTGGGCAAGTGGAGGAAAGCAAAAATTTGAGAGCTGAGTTGGAACAAATGAAGAAATCAATTGAGGAAGCTGAAATGCGGATACTAAGAGGAAATGTGGAAAGAAATGAGCTGGTAGGTACAATTTCTATTCTTAAGAAGGAAGCAGAGAAGTCATTGGAGGGTTTGAATAGAATGCAGCATCTCAAAGATGAAAAGGAGGTAACAATTGGACTCCTAAAGTCGGAGACAGAAGAGCTTAAAGCTCGGTGTAAGGACTTTAAACATGCTATATATGAGGATGAGGTAGAGAAAGAGAAACTTAGAAAGCAGGTATTTCAGCTTAAGGCTGACCTGAAAAAGAAGGAAGATGCGTTCACTACCGTTGAAAAGAAGCTCAAGGAAAGCAATGGGCGCGCAATAGTTTCTGATGGAATTAAAACTACTCAGAGAAACAATAAGTCTTTGCCAGTTGCTCGTGGTTCGAAAGAGGTTGCAAGTATGAAAGAGAGAATAAAATTGCTTGAGGGACAAATAAAATCAAAGGAAACCGCTTTGGAAACCTCAACTGCTGCATTTTTGGAGAAGGAAAAGGATCTTCAGAACATAATCGAAGAATTAGAGAGCAGGGTGGAAGAAATAAATCAGAATAGTGCATTTCAAATGGTAACTAAAGATGCCACTAGCGATGAGATTGCATTGGAGCAAGAACCAAGTTTTTCCACTGCTGATAGCAGGTACGGCGACTTATTAACTGAATTAGCATCACTGAAGGAGAAGAATATATCAATGGAAAGCGAACTAAAAGATATGCAAGAAAGATATTCGGAGATAAGTCTCAAATTCGCAGTGGTTGAAGGTGAAAGACAACAGCTCGTAATGACAATACGCAACCTCAAAAATTCCAAGAGGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04530 map04530
Pfam Domains
Protein Families
NT-C2

Protein Analysis

1111

Amino Acids

126.38

Weight (kDa)

5.1

Isoelectric Point (pI)

46.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 11 - 146 1.3e-21 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2166
Acc36I ACCTGC 3 cut(s) 773, 2737, 3141
AccBSI CCGCTC 1 cut(s) 2009
AccI GTMKAC 1 cut(s) 258
AccII CGCG 3 cut(s) 225, 649, 2832
AclI AACGTT 1 cut(s) 608
AclWI GGATC 9 cut(s) 47, 428, 604, 662, 667, 996, 1128, 1398, 3018
AcoI YGGCCR 1 cut(s) 2332
AcuI CTGAAG 6 cut(s) 849, 1700, 1713, 2048, 2999, 3206
AfaI GTAC 6 cut(s) 708, 805, 1242, 1610, 2557, 3155
AfiI CCNNNNNNNGG 5 cut(s) 236, 414, 1660, 2011, 2659
AflII CTTAAG 4 cut(s) 502, 1598, 2570, 2759
AflIII ACRYGT 1 cut(s) 1970
AjnI CCWGG 3 cut(s) 1248, 2214, 2376
AloI GAACNNNNNNTCC 4 cut(s) 1881, 1913, 2774, 2806
Alw21I GWGCWC 4 cut(s) 576, 1407, 1497, 2214
Alw26I GTCTC 8 cut(s) 277, 858, 1239, 1476, 1626, 2231, 2657, 3257
AlwI GGATC 9 cut(s) 47, 428, 604, 662, 667, 996, 1128, 1398, 3018
Ama87I CYCGRG 2 cut(s) 1433, 1496
AoxI GGCC 3 cut(s) 1301, 2097, 2332
ApeKI GCWGC 5 cut(s) 1322, 1513, 2326, 2614, 2987
ArsI GACNNNNNNTTYG 2 cut(s) 240, 272
AseI ATTAAT 1 cut(s) 549
Asp700I GAANNNNTTC 1 cut(s) 2073
AspLEI GCGC 3 cut(s) 2167, 2832, 2834
AspS9I GGNCC 2 cut(s) 281, 1171
AsuC2I CCSGG 3 cut(s) 238, 479, 1327
AsuHPI GGTGA 3 cut(s) 91, 1472, 3287
AsuII TTCGAA 3 cut(s) 1865, 2077, 2900
AsuNHI GCTAGC 1 cut(s) 1631
AvaI CYCGRG 2 cut(s) 1433, 1496
AvaII GGWCC 2 cut(s) 281, 1171
BanII GRGCYC 3 cut(s) 576, 1497, 2214
BauI CACGAG 1 cut(s) 2892
BbsI GAAGAC 4 cut(s) 43, 855, 1211, 2086
Bbv12I GWGCWC 4 cut(s) 576, 1407, 1497, 2214
BbvI GCAGC 5 cut(s) 1309, 1525, 2338, 2626, 2974
BccI CCATC 7 cut(s) 12, 180, 1349, 2009, 2089, 2343, 2840
BceAI ACGGC 2 cut(s) 2433, 3172
BcgI CGANNNNNNTGC 2 cut(s) 3092, 3126
BciT130I CCWGG 3 cut(s) 1250, 2216, 2378
BciVI GTATCC 2 cut(s) 508, 2511
BcnI CCSGG 3 cut(s) 238, 479, 1327
BcoDI GTCTC 8 cut(s) 277, 858, 1239, 1476, 1626, 2231, 2657, 3257
BfaI CTAG 9 cut(s) 294, 908, 912, 935, 1571, 1632, 2031, 2180, 3099
BfmI CTRYAG 6 cut(s) 59, 162, 780, 1124, 1312, 1474
BfrI CTTAAG 4 cut(s) 502, 1598, 2570, 2759
BfuAI ACCTGC 3 cut(s) 773, 2737, 3141
BfuI GTATCC 2 cut(s) 508, 2511
BisI GCNGC 7 cut(s) 454, 628, 1323, 1514, 2327, 2615, 2988
BlsI GCNGC 7 cut(s) 455, 629, 1324, 1515, 2328, 2616, 2989
BmcAI AGTACT 1 cut(s) 805
Bme1390I CCNGG 6 cut(s) 238, 479, 1250, 1327, 2216, 2378
Bme18I GGWCC 2 cut(s) 281, 1171
BmeT110I CYCGRG 2 cut(s) 1433, 1496
BmgT120I GGNCC 2 cut(s) 281, 1171
BmiI GGNNCC 2 cut(s) 120, 1103
BmrFI CCNGG 6 cut(s) 238, 479, 1250, 1327, 2216, 2378
BmtI GCTAGC 1 cut(s) 1635
BpiI GAAGAC 4 cut(s) 43, 855, 1211, 2086
BpmI CTGGAG 5 cut(s) 1184, 1406, 1784, 1946, 2198
Bpu14I TTCGAA 3 cut(s) 1865, 2077, 2900
BpuEI CTTGAG 4 cut(s) 356, 1751, 2798, 2960
BpuMI CCSGG 3 cut(s) 238, 479, 1327
BsaBI GATNNNNATC 1 cut(s) 753
BsaI GGTCTC 2 cut(s) 858, 1239
BsaJI CCNNGG 4 cut(s) 288, 336, 477, 2376
BsaWI WCCGGW 1 cut(s) 196
Bsc4I CCNNNNNNNGG 5 cut(s) 236, 414, 1660, 2011, 2659
Bse1I ACTGG 6 cut(s) 1105, 1527, 1767, 1929, 2151, 2885
Bse3DI GCAATG 4 cut(s) 101, 1417, 2145, 2830
Bse8I GATNNNNATC 1 cut(s) 753
BseBI CCWGG 3 cut(s) 1250, 2216, 2378
BseDI CCNNGG 4 cut(s) 288, 336, 477, 2376
BseGI GGATG 8 cut(s) 103, 133, 526, 587, 1084, 1372, 2001, 2722
BseJI GATNNNNATC 1 cut(s) 753
BseLI CCNNNNNNNGG 5 cut(s) 236, 414, 1660, 2011, 2659
BseMI GCAATG 4 cut(s) 101, 1417, 2145, 2830
BseMII CTCAG 9 cut(s) 89, 288, 717, 1166, 2046, 2100, 2414, 2463, 2876
BseNI ACTGG 6 cut(s) 1105, 1527, 1767, 1929, 2151, 2885
BsePI GCGCGC 1 cut(s) 2830
BseRI GAGGAG 1 cut(s) 2027
BseXI GCAGC 5 cut(s) 1309, 1525, 2338, 2626, 2974
BsgI GTGCAG 1 cut(s) 2111
Bsh1236I CGCG 3 cut(s) 225, 649, 2832
BshFI GGCC 3 cut(s) 1303, 2099, 2334
BsiHKAI GWGCWC 4 cut(s) 576, 1407, 1497, 2214
BsiHKCI CYCGRG 2 cut(s) 1433, 1496
BsiSI CCGG 4 cut(s) 197, 237, 479, 1326
BslFI GGGAC 3 cut(s) 1369, 1720, 2958
BslI CCNNNNNNNGG 5 cut(s) 236, 414, 1660, 2011, 2659
BsmAI GTCTC 8 cut(s) 277, 858, 1239, 1476, 1626, 2231, 2657, 3257
BsmBI CGTCTC 1 cut(s) 1626
BsmFI GGGAC 3 cut(s) 1369, 1720, 2958
BsmI GAATGC 3 cut(s) 1619, 1655, 2616
BsnI GGCC 3 cut(s) 1303, 2099, 2334
Bso31I GGTCTC 2 cut(s) 858, 1239
BsoBI CYCGRG 2 cut(s) 1433, 1496
Bsp119I TTCGAA 3 cut(s) 1865, 2077, 2900
Bsp1286I GDGCHC 4 cut(s) 576, 1407, 1497, 2214
BspANI GGCC 3 cut(s) 1303, 2099, 2334
BspCNI CTCAG 9 cut(s) 88, 287, 716, 1167, 2047, 2099, 2413, 2464, 2875
BspFNI CGCG 3 cut(s) 225, 649, 2832
BspHI TCATGA 1 cut(s) 829
BspLI GGNNCC 2 cut(s) 120, 1103
BspMAI CTGCAG 4 cut(s) 63, 784, 1128, 1316
BspMI ACCTGC 3 cut(s) 773, 2737, 3141
BspOI GCTAGC 1 cut(s) 1635
BspPI GGATC 9 cut(s) 47, 428, 604, 662, 667, 996, 1128, 1398, 3018
BspQI GCTCTTC 1 cut(s) 2664
BspT104I TTCGAA 3 cut(s) 1865, 2077, 2900
BspTI CTTAAG 4 cut(s) 502, 1598, 2570, 2759
BspTNI GGTCTC 2 cut(s) 858, 1239
BsrBI CCGCTC 1 cut(s) 2009
BsrDI GCAATG 4 cut(s) 101, 1417, 2145, 2830
BsrI ACTGG 6 cut(s) 1105, 1527, 1767, 1929, 2151, 2885
BssECI CCNNGG 4 cut(s) 288, 336, 477, 2376
BssHII GCGCGC 1 cut(s) 2830
BssSI CACGAG 1 cut(s) 2892
BssT1I CCWWGG 1 cut(s) 288
Bst2BI CACGAG 1 cut(s) 2892
Bst2UI CCWGG 3 cut(s) 1250, 2216, 2378
Bst4CI ACNGT 8 cut(s) 87, 145, 166, 211, 285, 852, 861, 2800
Bst6I CTCTTC 3 cut(s) 2165, 2286, 2664
BstAFI CTTAAG 4 cut(s) 502, 1598, 2570, 2759
BstAPI GCANNNNNTGC 1 cut(s) 779
BstBI TTCGAA 3 cut(s) 1865, 2077, 2900
BstC8I GCNNGC 8 cut(s) 227, 423, 1128, 1518, 1633, 1773, 1935, 2832
BstF5I GGATG 8 cut(s) 103, 133, 526, 587, 1084, 1372, 2001, 2722
BstFNI CGCG 3 cut(s) 225, 649, 2832
BstHHI GCGC 3 cut(s) 2167, 2832, 2834
BstMAI GTCTC 8 cut(s) 277, 858, 1239, 1476, 1626, 2231, 2657, 3257
BstNI CCWGG 3 cut(s) 1250, 2216, 2378
BstNSI RCATGY 5 cut(s) 404, 722, 1432, 1974, 2705
BstSCI CCNGG 6 cut(s) 236, 477, 1248, 1325, 2214, 2376
BstSFI CTRYAG 6 cut(s) 59, 162, 780, 1124, 1312, 1474
BstUI CGCG 3 cut(s) 225, 649, 2832
BstV1I GCAGC 5 cut(s) 1309, 1525, 2338, 2626, 2974
BstV2I GAAGAC 4 cut(s) 43, 855, 1211, 2086
BstX2I RGATCY 6 cut(s) 596, 667, 988, 1120, 1390, 3010
BstYI RGATCY 6 cut(s) 596, 667, 988, 1120, 1390, 3010
BsuI GTATCC 2 cut(s) 508, 2511
BsuRI GGCC 3 cut(s) 1303, 2099, 2334
BtgZI GCGATG 1 cut(s) 3117
BtsCI GGATG 8 cut(s) 103, 133, 526, 587, 1084, 1372, 2001, 2722
BtsI GCAGTG 3 cut(s) 2018, 3138, 3270
BtsIMutI CAGTG 7 cut(s) 1520, 2018, 2094, 2158, 3138, 3182, 3270
BveI ACCTGC 3 cut(s) 773, 2737, 3141
Cac8I GCNNGC 8 cut(s) 227, 423, 1128, 1518, 1633, 1773, 1935, 2832
CciI TCATGA 1 cut(s) 829
CfoI GCGC 3 cut(s) 2167, 2832, 2834
Cfr13I GGNCC 2 cut(s) 281, 1171
CseI GACGC 1 cut(s) 1216
Csp6I GTAC 6 cut(s) 707, 804, 1241, 1609, 2556, 3154
CspCI CAANNNNNGTGG 2 cut(s) 547, 582
CviAII CATG 9 cut(s) 401, 719, 774, 794, 830, 1429, 1553, 1971, 2702
CviQI GTAC 6 cut(s) 707, 804, 1241, 1609, 2556, 3154
DraI TTTAAA 1 cut(s) 2698
EaeI YGGCCR 1 cut(s) 2332
Eam1104I CTCTTC 3 cut(s) 2165, 2286, 2664
EarI CTCTTC 3 cut(s) 2165, 2286, 2664
EciI GGCGGA 1 cut(s) 1055
Ecl136II GAGCTC 3 cut(s) 574, 1495, 2212
Eco130I CCWWGG 1 cut(s) 288
Eco24I GRGCYC 3 cut(s) 576, 1497, 2214
Eco31I GGTCTC 2 cut(s) 858, 1239
Eco47I GGWCC 2 cut(s) 281, 1171
Eco53kI GAGCTC 3 cut(s) 574, 1495, 2212
Eco57I CTGAAG 6 cut(s) 849, 1700, 1713, 2048, 2999, 3206
Eco88I CYCGRG 2 cut(s) 1433, 1496
EcoICRI GAGCTC 3 cut(s) 574, 1495, 2212
EcoO109I RGGNCCY 1 cut(s) 1171
EcoRI GAATTC 2 cut(s) 376, 1895
EcoRII CCWGG 3 cut(s) 1248, 2214, 2376
EcoT14I CCWWGG 1 cut(s) 288
EcoT22I ATGCAT 2 cut(s) 103, 402
EcoT38I GRGCYC 3 cut(s) 576, 1497, 2214
ErhI CCWWGG 1 cut(s) 288
Esp3I CGTCTC 1 cut(s) 1626
FaeI CATG 9 cut(s) 404, 722, 777, 797, 833, 1432, 1556, 1974, 2705
FalI AAGNNNNNCTT 8 cut(s) 485, 517, 655, 687, 1581, 1613, 2721, 2753
FaqI GGGAC 3 cut(s) 1369, 1720, 2958
FatI CATG 9 cut(s) 400, 718, 773, 793, 829, 1428, 1552, 1970, 2701
FauI CCCGC 1 cut(s) 1109
FblI GTMKAC 1 cut(s) 258
Fnu4HI GCNGC 7 cut(s) 454, 628, 1323, 1514, 2327, 2615, 2988
FokI GGATG 8 cut(s) 110, 140, 533, 574, 1091, 1379, 1988, 2729
FriOI GRGCYC 3 cut(s) 576, 1497, 2214
Fsp4HI GCNGC 7 cut(s) 454, 628, 1323, 1514, 2327, 2615, 2988
FspBI CTAG 9 cut(s) 294, 908, 912, 935, 1571, 1632, 2031, 2180, 3099
FspI TGCGCA 1 cut(s) 2166
GlaI GCGC 3 cut(s) 2166, 2831, 2833
GluI GCNGC 7 cut(s) 454, 628, 1323, 1514, 2327, 2615, 2988
GsuI CTGGAG 5 cut(s) 1184, 1406, 1784, 1946, 2198
HaeIII GGCC 3 cut(s) 1303, 2099, 2334
HapII CCGG 4 cut(s) 197, 237, 479, 1326
HgaI GACGC 1 cut(s) 1216
HhaI GCGC 3 cut(s) 2167, 2832, 2834
Hin1II CATG 9 cut(s) 404, 722, 777, 797, 833, 1432, 1556, 1974, 2705
Hin6I GCGC 3 cut(s) 2165, 2830, 2832
HinP1I GCGC 3 cut(s) 2165, 2830, 2832
HincII GTYRAC 1 cut(s) 2177
HindII GTYRAC 1 cut(s) 2177
HindIII AAGCTT 3 cut(s) 487, 1747, 1909
HinfI GANTC 9 cut(s) 193, 694, 1028, 1214, 1307, 1733, 2082, 2173, 2649
HpaII CCGG 4 cut(s) 197, 237, 479, 1326
HphI GGTGA 3 cut(s) 91, 1472, 3287
Hpy166II GTNNAC 4 cut(s) 259, 1193, 2177, 2793
Hpy8I GTNNAC 4 cut(s) 259, 1193, 2177, 2793
HpyCH4III ACNGT 8 cut(s) 87, 145, 166, 211, 285, 852, 861, 2800
HpyCH4IV ACGT 3 cut(s) 608, 1072, 1871
HpySE526I ACGT 3 cut(s) 608, 1072, 1871
Hsp92II CATG 9 cut(s) 404, 722, 777, 797, 833, 1432, 1556, 1974, 2705
HspAI GCGC 3 cut(s) 2165, 2830, 2832
LguI GCTCTTC 1 cut(s) 2664
LmnI GCTCC 9 cut(s) 1203, 1264, 1402, 1510, 1990, 2196, 2217, 3118, 3327
Lsp1109I GCAGC 5 cut(s) 1309, 1525, 2338, 2626, 2974
MaeI CTAG 9 cut(s) 294, 908, 912, 935, 1571, 1632, 2031, 2180, 3099
MaeII ACGT 3 cut(s) 608, 1072, 1871
MaeIII GTNAC 8 cut(s) 79, 170, 403, 695, 1804, 1966, 2638, 3082
MbiI CCGCTC 1 cut(s) 2009
MfeI CAATTG 2 cut(s) 2496, 2643
MflI RGATCY 6 cut(s) 596, 667, 988, 1120, 1390, 3010
MhlI GDGCHC 4 cut(s) 576, 1407, 1497, 2214
MlyI GAGTC 5 cut(s) 703, 1742, 2076, 2182, 2643
MmeI TCCRAC 4 cut(s) 699, 1320, 2457, 2640
Mph1103I ATGCAT 2 cut(s) 103, 402
MroXI GAANNNNTTC 1 cut(s) 2073
MslI CAYNNNNRTG 2 cut(s) 717, 1410
MspA1I CMGCKG 2 cut(s) 278, 2054
MspCI CTTAAG 4 cut(s) 502, 1598, 2570, 2759
MspI CCGG 4 cut(s) 197, 237, 479, 1326
MspR9I CCNGG 6 cut(s) 238, 479, 1250, 1327, 2216, 2378
MunI CAATTG 2 cut(s) 2496, 2643
Mva1269I GAATGC 3 cut(s) 1619, 1655, 2616
MvaI CCWGG 3 cut(s) 1250, 2216, 2378
MvnI CGCG 3 cut(s) 225, 649, 2832
NciI CCSGG 3 cut(s) 238, 479, 1327
NheI GCTAGC 1 cut(s) 1631
NlaIII CATG 9 cut(s) 404, 722, 777, 797, 833, 1432, 1556, 1974, 2705
NlaIV GGNNCC 2 cut(s) 120, 1103
NmeAIII GCCGAG 2 cut(s) 361, 2310
NmuCI GTSAC 4 cut(s) 79, 170, 695, 1804
NsbI TGCGCA 1 cut(s) 2166
NsiI ATGCAT 2 cut(s) 103, 402
NspI RCATGY 5 cut(s) 404, 722, 1432, 1974, 2705
NspV TTCGAA 3 cut(s) 1865, 2077, 2900
PaeR7I CTCGAG 2 cut(s) 1433, 1496
PagI TCATGA 1 cut(s) 829
PauI GCGCGC 1 cut(s) 2830
PciI ACATGT 1 cut(s) 1970
PciSI GCTCTTC 1 cut(s) 2664
PctI GAATGC 3 cut(s) 1619, 1655, 2616
PdmI GAANNNNTTC 1 cut(s) 2073
PfeI GAWTC 4 cut(s) 193, 1028, 1214, 1307
PfoI TCCNGGA 1 cut(s) 2214
PkrI GCNGC 7 cut(s) 455, 629, 1324, 1515, 2328, 2616, 2989
PleI GAGTC 5 cut(s) 702, 1741, 2076, 2181, 2643
PpsI GAGTC 5 cut(s) 702, 1741, 2076, 2181, 2643
PpuMI RGGWCCY 1 cut(s) 1171
PscI ACATGT 1 cut(s) 1970
PshBI ATTAAT 1 cut(s) 549
Psp124BI GAGCTC 3 cut(s) 576, 1497, 2214
Psp1406I AACGTT 1 cut(s) 608
Psp5II RGGWCCY 1 cut(s) 1171
Psp6I CCWGG 3 cut(s) 1248, 2214, 2376
PspGI CCWGG 3 cut(s) 1248, 2214, 2376
PspN4I GGNNCC 2 cut(s) 120, 1103
PspPI GGNCC 2 cut(s) 281, 1171
PspPPI RGGWCCY 1 cut(s) 1171
PsrI GAACNNNNNNTAC 2 cut(s) 102, 134
PstI CTGCAG 4 cut(s) 63, 784, 1128, 1316
PsuI RGATCY 6 cut(s) 596, 667, 988, 1120, 1390, 3010
PteI GCGCGC 1 cut(s) 2830
PvuII CAGCTG 2 cut(s) 278, 2054
RsaI GTAC 6 cut(s) 708, 805, 1242, 1610, 2557, 3155
RsaNI GTAC 6 cut(s) 707, 804, 1241, 1609, 2556, 3154
RseI CAYNNNNRTG 2 cut(s) 717, 1410
SacI GAGCTC 3 cut(s) 576, 1497, 2214
SapI GCTCTTC 1 cut(s) 2664
SatI GCNGC 7 cut(s) 454, 628, 1323, 1514, 2327, 2615, 2988
Sau96I GGNCC 2 cut(s) 281, 1171
SbfI CCTGCAGG 1 cut(s) 1316
ScaI AGTACT 1 cut(s) 805
SchI GAGTC 5 cut(s) 703, 1742, 2076, 2182, 2643
ScrFI CCNGG 6 cut(s) 238, 479, 1250, 1327, 2216, 2378
SdaI CCTGCAGG 1 cut(s) 1316
SduI GDGCHC 4 cut(s) 576, 1407, 1497, 2214
SfcI CTRYAG 6 cut(s) 59, 162, 780, 1124, 1312, 1474
Sfr274I CTCGAG 2 cut(s) 1433, 1496
SfuI TTCGAA 3 cut(s) 1865, 2077, 2900
SinI GGWCC 2 cut(s) 281, 1171
SlaI CTCGAG 2 cut(s) 1433, 1496
SmiMI CAYNNNNRTG 2 cut(s) 717, 1410
Sse8387I CCTGCAGG 1 cut(s) 1316
SspI AATATT 1 cut(s) 1944
SspMI CTAG 9 cut(s) 294, 908, 912, 935, 1571, 1632, 2031, 2180, 3099
SstI GAGCTC 3 cut(s) 576, 1497, 2214
StyD4I CCNGG 6 cut(s) 236, 477, 1248, 1325, 2214, 2376
StyI CCWWGG 1 cut(s) 288
TaaI ACNGT 8 cut(s) 87, 145, 166, 211, 285, 852, 861, 2800
TaiI ACGT 3 cut(s) 611, 1075, 1874
TatI WGTACW 2 cut(s) 706, 803
TauI GCSGC 2 cut(s) 456, 630
TfiI GAWTC 4 cut(s) 193, 1028, 1214, 1307
TscAI CASTG 7 cut(s) 1527, 2025, 2094, 2158, 3145, 3189, 3270
TseFI GTSAC 4 cut(s) 79, 170, 695, 1804
TseI GCWGC 5 cut(s) 1322, 1513, 2326, 2614, 2987
Tsp45I GTSAC 4 cut(s) 79, 170, 695, 1804
TspDTI ATGAA 9 cut(s) 219, 537, 1155, 1179, 1656, 2133, 2501, 2643, 2933
TspGWI ACGGA 4 cut(s) 56, 533, 1178, 1850
TspRI CASTG 7 cut(s) 1527, 2025, 2094, 2158, 3145, 3189, 3270
Vha464I CTTAAG 4 cut(s) 502, 1598, 2570, 2759
VpaK11BI GGWCC 2 cut(s) 281, 1171
VspI ATTAAT 1 cut(s) 549
XceI RCATGY 5 cut(s) 404, 722, 1432, 1974, 2705
XhoI CTCGAG 2 cut(s) 1433, 1496
XmiI GTMKAC 1 cut(s) 258
XmnI GAANNNNTTC 1 cut(s) 2073
XspI CTAG 9 cut(s) 294, 908, 912, 935, 1571, 1632, 2031, 2180, 3099
ZrmI AGTACT 1 cut(s) 805
Zsp2I ATGCAT 2 cut(s) 103, 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.