MD14G1099400.v1.1

N-terminal C2 in EEIG1 and EHBP1 proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0007404
Physical Location & Seq
Forward (+)
15307313 .. 15312249
4937 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1099400.v1.1.491

Sequence Viewer

Length: 3333 bp
ATGTTCAAGTCGGCGCGATGGAGAACCGACAAGAACAAGATCATGGAAGTCTTCAAATTGCAGTTCCATGCAACTCAGGTGACACAGTTGGGTGGGGACGCATTGCTGGTTTCTGTGATTCCCGTGGATGTGGGGAAGCCAACTGTGAAATCAGAGAAAGCTACAGTTCGAGACGGGAGCTGTCGATGGGAAAATCCGGTCTGTGAAACAGTTAAATTCGTCCGTGAGCCAAGAACTGGGAAGATCAAAGAGAGTGTCTACAACTTTGTTGTCTCAAGTGGATCATCGAAGGGTAGTGTTCTTGGGGAAGTTTCTGTCGATTTTGCTGACTATGCCGAGGCTACTAAATCTTCCTGCGTCGCTCTTCCCCTTAAGAATTCAAATTCCAATGCAATTATGCATGTTACCATTCAAAGGTTGGAAGCAAATGTTGATCCGAGAGAGGTAGAAGGCGGTGAAGAGGCAAATGTTAACTCCGAAGATAGAAGCTTCAAGAACCTCTTAAGCAATAAGGATACTGATGAAAGCGTTTCAATTGATGAGGGAATTAATAGAACCATCCAAATTGCTGAGCTCAACTCCAGAGCATCTATCGGATCGGACATTACATTGTCAAGTTCTGACAGCGGCTCTGGACTTGGTACTCCACGAGAACTTGGATCAAGAGACCCTTCCAACTTTCTGTCGTCTCTGAGTCACACCTCAGTACAACATAAACCTGATGTTTATGCCCAAACAAATATCTATGACGAGCATCAGCGGTCACAATGGGCATGGTCTGCAGACTCAGAACATGGAGCAAGTACTGATGGTTCAACAAAGAGTTCTCATGAAACCCTTCTAAGAGAACGGTCTCAACAGTCTTCAGATGACAACATTGAAAAGCTAAAGGCGGAGCTTCTTGTTCTAGTTAGACAAGCCAATATGTCAGAGCTAGAATTACAAACATTAAGGAAGCAGATAGTAAAAGAGACCAAAAAGGGGCAGGATCTTTCGAGAGAAGTCATTAGCTTGAAAGAGGAACGAGATTCTTTCAAGGCAGAATGTGAGAAACTAAAGTCCTTTTGGAAACGTGTGGATGATGCAAAAGTTAAAAACAGGTTCCAGTTAGAAGGTGGGGATCTACCGGCTCTTGTAGATGAAATCAGACAAGAACTGAGTTGTGAGAAGGACCTGACTTTCAATCTCCGCTTACAGCTCCAGAAGACACAAGAATCAAATTCTGATTTAATCCTTGCTGTACAAGACCTGGAGGAAATTTTGGAGCAGAAGAATAGTGAAATAGCAAATCTTTCCAACAGACCAGAATCCTGCGGTTATGCTGCAGGGTTGAAGGAAACCATCTTAAAAGGTGGGACAAGTGAGGATGAGGAGCAGATGGAGCTGGAGGATCTTGTTAAGGACCACAGCAATGCCATGGAAACACACCTGCTCGAGAGACAGATTGCCGAACTTTATGGTGAAATAGAAGTCTATAGGCGAGACAAAGATGTGCTCGAGATGCAAATGGAGCAGCTTGCACTTGACTATGAGATATTGAAACAGGAAAACCATGACATCTTATATAAACTAGAGCAAAGCCAACTGCAAGAACAACTTAGGATGCAGTACGAATGCTCGTCTCCCTCTGCTAGCATAAATGAGCTTGAATACCAAGTTGAGGATTTGGAAACAGAATTGAAGAAGCAGTCTGAAGATTTTTCAAATTCATTGGCTACCATAAGGGAACTTGAATCCCATATCAAAAGCTTGGAGGAAGAACTAAAGAAGCAGGCACAAGTATATGAGGCTGATTTAGAGGCTGTAACATGTGCCAAAGTTGCGGGTTTGGAAACAGAACTAAGGAAGCAGTCTGAAGATTTTTCGAATTTGTTGGCTACCATAAAGGAACTTGAATTCCATCTCAAAAGCTTGGAGGAAGAACTGGAGAAACAGGCGCAAGTATTTGAAGCTGATTTGGAGGCCGTAACATGTGCCAAAGTTGAGCAGGAGCAAAGAGCCATCCGAGCAGAGGACGCACTGCGAAAAACTAGATCAAAAAATGCTAATACAGCTGAGAGGCTTCAGGAAGAATTCCGAAGACTCTCTGTTCAAATGGCCTCGACATTTGATGCAAATGAAAAGGTAGCTCTGAAAGCAATGACAGAAGCTAGTGAACTGTGTGGGCAGAAGAGTCACCTAGAAGAACTGCTCCAGAAAACGAAGGATGAGCTCCAGGAAGTTAGAAATGATTATGAGGCAAGATTGCAGAAGCTCTCGAACCACTTAGACGAGAAAACCAAAGAGATGGAAGAGATTTTACTGGAAATGGACAACAATTACAAGCAGCTCGACCATCAGCAGAAACAAGAGGAAGAAGTTAAGGGAAATATCTCCCAGGTGATCTTACAACTCAAATCTCAGATTCAAAGGCTTACAGAAGAGAATAACAGCCTTTCTGAGCAAGTGGAGGAAAGCAAAAATTTGAGAGCTGATTTGGAACAAATGAAGAAATCAATCGAGGAAGCTGAGATGCGGATACAAAGAGGAAATGCAGAAAGAAATGAGCTGGTAAGTAAAATTTCTACTCTTAAGAAGGAAGCAGAGAAGTCATTGGAGGATTTGAATACAATGCGGCATCTCAAAGATGAAAAGGAGGTGACAGTTGGACTCCTAAAATCAGAGATAGAGGAGCTTAAAGCTCGGTGTAAAGACTTTAAACATGCTATATATGAGGATGAGGTTGAGAAGGAGAAACTTAGAAAGCAGGTATTCCAGCTTAAGGCTGACCTGAAAAAGAAGGAAGATGCACTCAGTACCGCTGAAAAGAAACTCAAGGAAAGCAACGGGCGTTCAATAGTTTCTGATGGAATTAAAATAACTCAGAGAAACATCAAGTCTTTGCCAGTTGCTCGTGGTTCGAAAGAAGTTGCATGTATGAAAGAAAGAATAAAATTGCTCGAGGGACAAATAAAATCGAAGGAAGCTGCTTTGGGAAACTCAACTGCTTCATTTTTGGAGAAGGAAAAGGATCTTCAGAACATAATCGAAAAGTTAGAGAGCACGGTGGAAGAAATAAATCAGAATAGTGCATTTCAAAAGGTAACTGAAGATGCCGGTAGTGATAAAATTGCATCAGAACAAGAACTGAGACTTTTCACTGCTGATAACAGATACAGCGACTTATTAACTGAATTAGCATCATTGAAAGAGAGGAATATATCAATGGAAAGTGAACTAAAAGAGATGCAAGAGAGATATTCAGAAATAAGTCTCAAATTCGCAGAGGTCGAAGGTGAAAGACAACAGCTCGTAATGACAGTACGCAACCTCAAAAATTCCAAGAGGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04530 map04530
Pfam Domains
Protein Families
NT-C2

Protein Analysis

1111

Amino Acids

126.46

Weight (kDa)

5.03

Isoelectric Point (pI)

47.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 11 - 146 1.4e-22 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1437
Acc36I ACCTGC 2 cut(s) 1437, 2737
AccB7I CCANNNNNTGG 1 cut(s) 236
AccI GTMKAC 1 cut(s) 258
AccII CGCG 1 cut(s) 16
AclWI GGATC 8 cut(s) 289, 428, 604, 667, 996, 1128, 1398, 3018
AcuI CTGAAG 6 cut(s) 849, 1713, 1875, 2048, 2999, 3108
AfaI GTAC 7 cut(s) 643, 708, 805, 1242, 1610, 2797, 3303
AfiI CCNNNNNNNGG 6 cut(s) 236, 414, 981, 1660, 2011, 2761
AflII CTTAAG 4 cut(s) 371, 502, 2570, 2759
AflIII ACRYGT 3 cut(s) 1072, 1808, 1970
AjnI CCWGG 3 cut(s) 1248, 2214, 2376
AloI GAACNNNNNNTCC 2 cut(s) 1881, 1913
Alw21I GWGCWC 4 cut(s) 576, 1497, 2214, 3044
AlwI GGATC 8 cut(s) 289, 428, 604, 667, 996, 1128, 1398, 3018
Ama87I CYCGRG 3 cut(s) 1433, 1496, 2939
AoxI GGCC 2 cut(s) 1962, 2097
ApeKI GCWGC 4 cut(s) 1322, 1513, 2326, 2966
ArsI GACNNNNNNTTYG 2 cut(s) 240, 272
AseI ATTAAT 1 cut(s) 549
Asp700I GAANNNNTTC 1 cut(s) 837
AspLEI GCGC 2 cut(s) 16, 1939
AspS9I GGNCC 2 cut(s) 1171, 1402
AsuHPI GGTGA 7 cut(s) 91, 467, 1472, 2168, 2392, 2650, 3287
AsuII TTCGAA 2 cut(s) 1865, 2900
AsuNHI GCTAGC 1 cut(s) 1631
AvaI CYCGRG 3 cut(s) 1433, 1496, 2939
AvaII GGWCC 2 cut(s) 1171, 1402
BanII GRGCYC 2 cut(s) 576, 2214
BarI GAAGNNNNNNTAC 2 cut(s) 2283, 2315
BauI CACGAG 2 cut(s) 648, 2892
BbsI GAAGAC 4 cut(s) 43, 855, 1211, 2086
Bbv12I GWGCWC 4 cut(s) 576, 1497, 2214, 3044
BbvI GCAGC 4 cut(s) 1309, 1525, 2338, 2953
BceAI ACGGC 1 cut(s) 1949
BciT130I CCWGG 3 cut(s) 1250, 2216, 2378
BciVI GTATCC 2 cut(s) 508, 2511
BfaI CTAG 7 cut(s) 908, 935, 1571, 1632, 2031, 2151, 2180
BfmI CTRYAG 4 cut(s) 162, 780, 1323, 1474
BfrI CTTAAG 4 cut(s) 371, 502, 2570, 2759
BfuAI ACCTGC 2 cut(s) 1437, 2737
BfuI GTATCC 2 cut(s) 508, 2511
BisI GCNGC 6 cut(s) 628, 1323, 1514, 2327, 2615, 2967
BlpI GCTNAGC 1 cut(s) 570
BlsI GCNGC 6 cut(s) 629, 1324, 1515, 2328, 2616, 2968
BmcAI AGTACT 1 cut(s) 805
Bme1390I CCNGG 3 cut(s) 1250, 2216, 2378
Bme18I GGWCC 2 cut(s) 1171, 1402
BmeT110I CYCGRG 3 cut(s) 1433, 1496, 2939
BmgT120I GGNCC 2 cut(s) 1171, 1402
BmiI GGNNCC 1 cut(s) 1103
BmrFI CCNGG 3 cut(s) 1250, 2216, 2378
BmrI ACTGGG 1 cut(s) 246
BmtI GCTAGC 1 cut(s) 1635
BmuI ACTGGG 1 cut(s) 246
BpiI GAAGAC 4 cut(s) 43, 855, 1211, 2086
BplI GAGNNNNNCTC 2 cut(s) 563, 595
BpmI CTGGAG 7 cut(s) 565, 1184, 1271, 1406, 1946, 2177, 2198
Bpu1102I GCTNAGC 1 cut(s) 570
Bpu14I TTCGAA 2 cut(s) 1865, 2900
BpuEI CTTGAG 2 cut(s) 259, 2798
BsaI GGTCTC 3 cut(s) 660, 858, 965
BsaJI CCNNGG 4 cut(s) 123, 336, 1416, 2376
BsaWI WCCGGW 1 cut(s) 196
Bsc4I CCNNNNNNNGG 6 cut(s) 236, 414, 981, 1660, 2011, 2761
Bse118I RCCGGY 2 cut(s) 1126, 3095
Bse1I ACTGG 5 cut(s) 241, 1105, 1929, 2307, 2885
Bse3DI GCAATG 3 cut(s) 101, 1417, 2145
BseBI CCWGG 3 cut(s) 1250, 2216, 2378
BseDI CCNNGG 4 cut(s) 123, 336, 1416, 2376
BseGI GGATG 8 cut(s) 133, 558, 1084, 1372, 1608, 2001, 2212, 2722
BseLI CCNNNNNNNGG 6 cut(s) 236, 414, 981, 1660, 2011, 2761
BseMI GCAATG 3 cut(s) 101, 1417, 2145
BseNI ACTGG 5 cut(s) 241, 1105, 1929, 2307, 2885
BseRI GAGGAG 2 cut(s) 1385, 2684
BseXI GCAGC 4 cut(s) 1309, 1525, 2338, 2953
Bsh1236I CGCG 1 cut(s) 16
BshFI GGCC 2 cut(s) 1964, 2099
BsiHKAI GWGCWC 4 cut(s) 576, 1497, 2214, 3044
BsiHKCI CYCGRG 3 cut(s) 1433, 1496, 2939
BsiSI CCGG 3 cut(s) 197, 1127, 3096
BslFI GGGAC 3 cut(s) 110, 1369, 2958
BslI CCNNNNNNNGG 6 cut(s) 236, 414, 981, 1660, 2011, 2761
BsmBI CGTCTC 3 cut(s) 165, 693, 1626
BsmFI GGGAC 3 cut(s) 110, 1369, 2958
BsmI GAATGC 1 cut(s) 1619
BsnI GGCC 2 cut(s) 1964, 2099
Bso31I GGTCTC 3 cut(s) 660, 858, 965
BsoBI CYCGRG 3 cut(s) 1433, 1496, 2939
Bsp119I TTCGAA 2 cut(s) 1865, 2900
Bsp1286I GDGCHC 4 cut(s) 576, 1497, 2214, 3044
Bsp1407I TGTACA 1 cut(s) 1240
Bsp1720I GCTNAGC 1 cut(s) 570
Bsp19I CCATGG 1 cut(s) 1416
BspANI GGCC 2 cut(s) 1964, 2099
BspFNI CGCG 1 cut(s) 16
BspHI TCATGA 1 cut(s) 829
BspLI GGNNCC 1 cut(s) 1103
BspMAI CTGCAG 2 cut(s) 784, 1327
BspMI ACCTGC 2 cut(s) 1437, 2737
BspOI GCTAGC 1 cut(s) 1635
BspPI GGATC 8 cut(s) 289, 428, 604, 667, 996, 1128, 1398, 3018
BspQI GCTCTTC 1 cut(s) 369
BspT104I TTCGAA 2 cut(s) 1865, 2900
BspTI CTTAAG 4 cut(s) 371, 502, 2570, 2759
BspTNI GGTCTC 3 cut(s) 660, 858, 965
BsrDI GCAATG 3 cut(s) 101, 1417, 2145
BsrFI RCCGGY 2 cut(s) 1126, 3095
BsrGI TGTACA 1 cut(s) 1240
BsrI ACTGG 5 cut(s) 241, 1105, 1929, 2307, 2885
BssAI RCCGGY 2 cut(s) 1126, 3095
BssECI CCNNGG 4 cut(s) 123, 336, 1416, 2376
BssSI CACGAG 2 cut(s) 648, 2892
BssT1I CCWWGG 1 cut(s) 1416
Bst2BI CACGAG 2 cut(s) 648, 2892
Bst2UI CCWGG 3 cut(s) 1250, 2216, 2378
Bst6I CTCTTC 5 cut(s) 369, 453, 2165, 2286, 2415
BstAFI CTTAAG 4 cut(s) 371, 502, 2570, 2759
BstAPI GCANNNNNTGC 1 cut(s) 779
BstAUI TGTACA 1 cut(s) 1240
BstBI TTCGAA 2 cut(s) 1865, 2900
BstC8I GCNNGC 3 cut(s) 1518, 1633, 1773
BstDSI CCRYGG 2 cut(s) 123, 1416
BstF5I GGATG 8 cut(s) 133, 558, 1084, 1372, 1608, 2001, 2212, 2722
BstFNI CGCG 1 cut(s) 16
BstHHI GCGC 2 cut(s) 16, 1939
BstNI CCWGG 3 cut(s) 1250, 2216, 2378
BstNSI RCATGY 5 cut(s) 404, 1812, 1974, 2705, 2916
BstSCI CCNGG 3 cut(s) 1248, 2214, 2376
BstSFI CTRYAG 4 cut(s) 162, 780, 1323, 1474
BstUI CGCG 1 cut(s) 16
BstV1I GCAGC 4 cut(s) 1309, 1525, 2338, 2953
BstV2I GAAGAC 4 cut(s) 43, 855, 1211, 2086
BstX2I RGATCY 4 cut(s) 988, 1120, 1390, 3010
BstXI CCANNNNNNTGG 1 cut(s) 2287
BstYI RGATCY 4 cut(s) 988, 1120, 1390, 3010
BsuI GTATCC 2 cut(s) 508, 2511
BsuRI GGCC 2 cut(s) 1964, 2099
BtgI CCRYGG 2 cut(s) 123, 1416
BtgZI GCGATG 1 cut(s) 31
BtsCI GGATG 8 cut(s) 133, 558, 1084, 1372, 1608, 2001, 2212, 2722
BtsI GCAGTG 2 cut(s) 2018, 3138
BtsIMutI CAGTG 2 cut(s) 2018, 3138
BveI ACCTGC 2 cut(s) 1437, 2737
Cac8I GCNNGC 3 cut(s) 1518, 1633, 1773
CciI TCATGA 1 cut(s) 829
CfoI GCGC 2 cut(s) 16, 1939
Cfr10I RCCGGY 2 cut(s) 1126, 3095
Cfr13I GGNCC 2 cut(s) 1171, 1402
CseI GACGC 3 cut(s) 107, 346, 2024
Csp6I GTAC 7 cut(s) 642, 707, 804, 1241, 1609, 2796, 3302
CviQI GTAC 7 cut(s) 642, 707, 804, 1241, 1609, 2796, 3302
DraI TTTAAA 1 cut(s) 2698
Eam1104I CTCTTC 5 cut(s) 369, 453, 2165, 2286, 2415
EarI CTCTTC 5 cut(s) 369, 453, 2165, 2286, 2415
EciI GGCGGA 1 cut(s) 908
Ecl136II GAGCTC 2 cut(s) 574, 2212
Eco130I CCWWGG 1 cut(s) 1416
Eco24I GRGCYC 2 cut(s) 576, 2214
Eco31I GGTCTC 3 cut(s) 660, 858, 965
Eco47I GGWCC 2 cut(s) 1171, 1402
Eco53kI GAGCTC 2 cut(s) 574, 2212
Eco57I CTGAAG 6 cut(s) 849, 1713, 1875, 2048, 2999, 3108
Eco88I CYCGRG 3 cut(s) 1433, 1496, 2939
EcoICRI GAGCTC 2 cut(s) 574, 2212
EcoO109I RGGNCCY 1 cut(s) 1171
EcoRI GAATTC 3 cut(s) 376, 1895, 2072
EcoRII CCWGG 3 cut(s) 1248, 2214, 2376
EcoT14I CCWWGG 1 cut(s) 1416
EcoT22I ATGCAT 1 cut(s) 402
EcoT38I GRGCYC 2 cut(s) 576, 2214
ErhI CCWWGG 1 cut(s) 1416
Esp3I CGTCTC 3 cut(s) 165, 693, 1626
FalI AAGNNNNNCTT 6 cut(s) 485, 517, 655, 687, 1581, 1613
FaqI GGGAC 3 cut(s) 110, 1369, 2958
FauI CCCGC 1 cut(s) 1816
FblI GTMKAC 1 cut(s) 258
Fnu4HI GCNGC 6 cut(s) 628, 1323, 1514, 2327, 2615, 2967
FokI GGATG 8 cut(s) 140, 545, 1091, 1379, 1615, 1988, 2219, 2729
FriOI GRGCYC 2 cut(s) 576, 2214
Fsp4HI GCNGC 6 cut(s) 628, 1323, 1514, 2327, 2615, 2967
FspBI CTAG 7 cut(s) 908, 935, 1571, 1632, 2031, 2151, 2180
GlaI GCGC 2 cut(s) 15, 1938
GluI GCNGC 6 cut(s) 628, 1323, 1514, 2327, 2615, 2967
GsuI CTGGAG 7 cut(s) 565, 1184, 1271, 1406, 1946, 2177, 2198
HaeIII GGCC 2 cut(s) 1964, 2099
HapII CCGG 3 cut(s) 197, 1127, 3096
HgaI GACGC 3 cut(s) 107, 346, 2024
HhaI GCGC 2 cut(s) 16, 1939
Hin6I GCGC 2 cut(s) 14, 1937
HinP1I GCGC 2 cut(s) 14, 1937
HincII GTYRAC 1 cut(s) 472
HindII GTYRAC 1 cut(s) 472
HindIII AAGCTT 3 cut(s) 487, 1747, 1909
HpaI GTTAAC 1 cut(s) 472
HpaII CCGG 3 cut(s) 197, 1127, 3096
HphI GGTGA 7 cut(s) 91, 467, 1472, 2168, 2392, 2650, 3287
Hpy166II GTNNAC 4 cut(s) 259, 472, 2156, 3215
Hpy8I GTNNAC 4 cut(s) 259, 472, 2156, 3215
Hpy99I CGWCG 1 cut(s) 362
HpyCH4IV ACGT 1 cut(s) 1072
HpySE526I ACGT 1 cut(s) 1072
HspAI GCGC 2 cut(s) 14, 1937
KspAI GTTAAC 1 cut(s) 472
LguI GCTCTTC 1 cut(s) 369
Lsp1109I GCAGC 4 cut(s) 1309, 1525, 2338, 2953
MaeI CTAG 7 cut(s) 908, 935, 1571, 1632, 2031, 2151, 2180
MaeII ACGT 1 cut(s) 1072
MaeIII GTNAC 9 cut(s) 79, 403, 695, 762, 1804, 1966, 2174, 2638, 3082
MfeI CAATTG 1 cut(s) 534
MflI RGATCY 4 cut(s) 988, 1120, 1390, 3010
MhlI GDGCHC 4 cut(s) 576, 1497, 2214, 3044
MlyI GAGTC 5 cut(s) 703, 779, 2076, 2182, 2643
MmeI TCCRAC 4 cut(s) 399, 699, 1320, 2626
Mph1103I ATGCAT 1 cut(s) 402
MroXI GAANNNNTTC 1 cut(s) 837
MslI CAYNNNNRTG 1 cut(s) 1410
MspA1I CMGCKG 4 cut(s) 627, 760, 2054, 2801
MspCI CTTAAG 4 cut(s) 371, 502, 2570, 2759
MspI CCGG 3 cut(s) 197, 1127, 3096
MspR9I CCNGG 3 cut(s) 1250, 2216, 2378
MunI CAATTG 1 cut(s) 534
Mva1269I GAATGC 1 cut(s) 1619
MvaI CCWGG 3 cut(s) 1250, 2216, 2378
MvnI CGCG 1 cut(s) 16
NcoI CCATGG 1 cut(s) 1416
NheI GCTAGC 1 cut(s) 1631
NlaIV GGNNCC 1 cut(s) 1103
NmeAIII GCCGAG 1 cut(s) 361
NmuCI GTSAC 5 cut(s) 79, 695, 762, 2174, 2638
NsiI ATGCAT 1 cut(s) 402
NspI RCATGY 5 cut(s) 404, 1812, 1974, 2705, 2916
NspV TTCGAA 2 cut(s) 1865, 2900
PaeR7I CTCGAG 3 cut(s) 1433, 1496, 2939
PagI TCATGA 1 cut(s) 829
PaqCI CACCTGC 1 cut(s) 1437
PciI ACATGT 2 cut(s) 1808, 1970
PciSI GCTCTTC 1 cut(s) 369
PcsI WCGNNNNNNNCGW 1 cut(s) 3267
PctI GAATGC 1 cut(s) 1619
PdmI GAANNNNTTC 1 cut(s) 837
PfeI GAWTC 6 cut(s) 118, 1028, 1214, 1307, 1733, 2404
PflMI CCANNNNNTGG 1 cut(s) 236
PfoI TCCNGGA 1 cut(s) 2214
PkrI GCNGC 6 cut(s) 629, 1324, 1515, 2328, 2616, 2968
PleI GAGTC 5 cut(s) 702, 779, 2076, 2181, 2643
PpsI GAGTC 5 cut(s) 702, 779, 2076, 2181, 2643
PpuMI RGGWCCY 1 cut(s) 1171
PscI ACATGT 2 cut(s) 1808, 1970
PshBI ATTAAT 1 cut(s) 549
Psp124BI GAGCTC 2 cut(s) 576, 2214
Psp5II RGGWCCY 1 cut(s) 1171
Psp6I CCWGG 3 cut(s) 1248, 2214, 2376
PspGI CCWGG 3 cut(s) 1248, 2214, 2376
PspN4I GGNNCC 1 cut(s) 1103
PspPI GGNCC 2 cut(s) 1171, 1402
PspPPI RGGWCCY 1 cut(s) 1171
PspXI VCTCGAGB 1 cut(s) 2939
PsrI GAACNNNNNNTAC 2 cut(s) 796, 828
PstI CTGCAG 2 cut(s) 784, 1327
PsuI RGATCY 4 cut(s) 988, 1120, 1390, 3010
PvuII CAGCTG 1 cut(s) 2054
RsaI GTAC 7 cut(s) 643, 708, 805, 1242, 1610, 2797, 3303
RsaNI GTAC 7 cut(s) 642, 707, 804, 1241, 1609, 2796, 3302
RseI CAYNNNNRTG 1 cut(s) 1410
SacI GAGCTC 2 cut(s) 576, 2214
SapI GCTCTTC 1 cut(s) 369
SatI GCNGC 6 cut(s) 628, 1323, 1514, 2327, 2615, 2967
Sau96I GGNCC 2 cut(s) 1171, 1402
ScaI AGTACT 1 cut(s) 805
SchI GAGTC 5 cut(s) 703, 779, 2076, 2182, 2643
ScrFI CCNGG 3 cut(s) 1250, 2216, 2378
SduI GDGCHC 4 cut(s) 576, 1497, 2214, 3044
SfcI CTRYAG 4 cut(s) 162, 780, 1323, 1474
Sfr274I CTCGAG 3 cut(s) 1433, 1496, 2939
SfuI TTCGAA 2 cut(s) 1865, 2900
SinI GGWCC 2 cut(s) 1171, 1402
SlaI CTCGAG 3 cut(s) 1433, 1496, 2939
SmiMI CAYNNNNRTG 1 cut(s) 1410
SmlI CTYRAG 9 cut(s) 274, 371, 502, 1433, 1496, 2570, 2759, 2813, 2939
SmoI CTYRAG 9 cut(s) 274, 371, 502, 1433, 1496, 2570, 2759, 2813, 2939
SspMI CTAG 7 cut(s) 908, 935, 1571, 1632, 2031, 2151, 2180
SstI GAGCTC 2 cut(s) 576, 2214
StyD4I CCNGG 3 cut(s) 1248, 2214, 2376
StyI CCWWGG 1 cut(s) 1416
TaiI ACGT 1 cut(s) 1075
TatI WGTACW 3 cut(s) 706, 803, 1240
TauI GCSGC 2 cut(s) 630, 2617
TfiI GAWTC 6 cut(s) 118, 1028, 1214, 1307, 1733, 2404
TscAI CASTG 2 cut(s) 2025, 3145
TseFI GTSAC 5 cut(s) 79, 695, 762, 2174, 2638
TseI GCWGC 4 cut(s) 1322, 1513, 2326, 2966
Tsp45I GTSAC 5 cut(s) 79, 695, 762, 2174, 2638
TspDTI ATGAA 9 cut(s) 537, 846, 1155, 1698, 2133, 2501, 2643, 2933, 2979
TspGWI ACGGA 1 cut(s) 212
TspRI CASTG 2 cut(s) 2025, 3145
Van91I CCANNNNNTGG 1 cut(s) 236
Vha464I CTTAAG 4 cut(s) 371, 502, 2570, 2759
VpaK11BI GGWCC 2 cut(s) 1171, 1402
VspI ATTAAT 1 cut(s) 549
XceI RCATGY 5 cut(s) 404, 1812, 1974, 2705, 2916
XcmI CCANNNNNNNNNTGG 2 cut(s) 415, 1112
XhoI CTCGAG 3 cut(s) 1433, 1496, 2939
XmiI GTMKAC 1 cut(s) 258
XmnI GAANNNNTTC 1 cut(s) 837
XspI CTAG 7 cut(s) 908, 935, 1571, 1632, 2031, 2151, 2180
ZrmI AGTACT 1 cut(s) 805
Zsp2I ATGCAT 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.