MD06G1079900.v1.1

Putative lipopolysaccharide-modifying enzyme.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0008951
Physical Location & Seq
Reverse (-)
19566300 .. 19568746
2447 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1079900.v1.1.491

Sequence Viewer

Length: 1566 bp
ATGTTGCGTGGATATCGCTTTGCCAGACCTTTCAAGTTTCAGGGAACAAGTTTAAAGGGATCTGCACCAGCTGCTGTTGCAACCAATACTATTCTCTTCGTGGGGCTATTCATCATTGCTTCGGCCACTCTATCTTCCTTTTGGGTTCAAACTTCCAATTTTCAGATTGGTAGTTCTACAAACCAAACCATAGTTGTCTCCGACAAGTTTAAAAAAACCCCTAAATCAGTTGAATTCCCACTCAACTGTTCCATTGGCAACAATGCTAACCAAACTCAGACCTGCCCAGCAAACTACCCTACAACCTTTAGCAATACTGATGAACTTGACCCGTCGCCAAACCCTTTGTGCCCGGATTATTTCCGGTTCATCCACCAGGATCTCATGCCATGGAAACGCACAGGAATCACAAGGGACATGGTGGAGAACGCAAAGAAGACAGCACATTTTAGGCTCGTGATTGTGAAGGGCAAGGTTTATGTTGAGAAGTACAAGCAGTCCATACAAACAAGGGATGTTTTTACCATATGGGGCATTTTGCAGCTTCTTAGGAGGTATCCTGGCAGACTACCTGACTTGGAATTCATGTTTGACTGTGATGACCAGCCGGTCATCCGATCGAGGGACTACCACGGACTGAACTCGACTCGGGTACCGCCACTGTTCCGGTATTGTGGTGATAGATGGACAAAGGACATCGTATTCCCTGATTGGTCCTTCTGGGGTTGGAATGAGATAAACATAAAACCATGGGAAGGTTTTTTGAAAGATGTCAAGAAAGGCAATCAAAGGATCAAATGGATGGACAGAGAACCTTATGCATACTGGAAAGGAAACCCCTTTGTTTCTGATACGAGGAAAGACTTACTCAAATGCAATGTCTCAGGGACACAGGACTGGAATGCTCGCCTATTCATCCAGGATTGGATTCTTGAATCTCAACAAGGCTTCAAGCAATCAAATGTAGCAGATCAATGCACACACAGGTACAAGATCTATATTGAGGGTTATGCATGGTCTGTGAGCGAGAAGTACATTCTAGCCTGTGATTCAGTAACATTGCTGGTAAAACCGAAGTACTATGATTTCTTCACAAGAGGTCTGCAACCAGTGCACCATTACTGGCCTGTAAGGCATGAAAACAAATGTAGATCCATTAAGTTTGCTGTGGATTGGGGCAACAACCACAAACAAAAGGCACAAGCAATTGGAAAAGCAGCAAGCAACTTCATTCAGGAGGAGCTAAAGATGGACTATGTGTATGACTACATGTTTCATCTGTTAAATGAATATGCTAAACTCTTAAGATTTGAGCCACGAATACCCAAGGGCACTACACATTTGTGCTCGGAGAGTATAGCTTGTCCTGCAGATGAGTCACCAAAGAAGTCTATGATAGAATCATTGGTTAAGAGTCCATCAGCATCAGACCCCTGCACCATGCCTCCTCCCTATGAACCCCGAGCTCTTGCAAAGTTATACAGGAGGAATATTAATTCAATTACACAAGTGCAACAGTGGGAAGATAAGTACTGGGAAAATCTCTCTAAGCAGCAACACCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
Glyco_transf_90

Protein Analysis

522

Amino Acids

60.77

Weight (kDa)

9.23

Isoelectric Point (pI)

44.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_90 PF05686 114 - 508 3.3e-191 Glycosyl transferase family 90
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 608
Acc36I ACCTGC 1 cut(s) 290
Acc65I GGTACC 1 cut(s) 652
AccB1I GGYRCC 1 cut(s) 652
AciI CCGC 1 cut(s) 656
AclWI GGATC 4 cut(s) 67, 387, 800, 1146
AcoI YGGCCR 1 cut(s) 123
AcsI RAATTY 2 cut(s) 233, 581
AfaI GTAC 6 cut(s) 491, 654, 989, 1034, 1079, 1532
AfiI CCNNNNNNNGG 2 cut(s) 622, 755
AflII CTTAAG 1 cut(s) 1303
AflIII ACRYGT 1 cut(s) 1269
AgsI TTSAA 7 cut(s) 34, 149, 233, 766, 935, 952, 1500
AjnI CCWGG 3 cut(s) 375, 559, 918
AleI CACNNNNGTG 1 cut(s) 1342
AluBI AGCT 5 cut(s) 71, 544, 1243, 1361, 1466
AluI AGCT 5 cut(s) 71, 544, 1243, 1361, 1466
Alw21I GWGCWC 3 cut(s) 1116, 1349, 1468
Alw26I GTCTC 2 cut(s) 202, 886
Alw44I GTGCAC 1 cut(s) 1112
AlwI GGATC 4 cut(s) 67, 387, 800, 1146
AlwNI CAGNNNCTG 1 cut(s) 74
Ama87I CYCGRG 2 cut(s) 648, 1461
AoxI GGCC 2 cut(s) 123, 1124
ApaLI GTGCAC 1 cut(s) 1112
ApeKI GCWGC 4 cut(s) 71, 541, 1217, 1552
ApoI RAATTY 2 cut(s) 233, 581
ArsI GACNNNNNNTTYG 2 cut(s) 864, 896
AseI ATTAAT 1 cut(s) 1494
Asp718I GGTACC 1 cut(s) 652
AspS9I GGNCC 1 cut(s) 714
AsuC2I CCSGG 1 cut(s) 353
AsuHPI GGTGA 2 cut(s) 689, 1371
AvaI CYCGRG 2 cut(s) 648, 1461
AvaII GGWCC 1 cut(s) 714
BaeGI GKGCMC 3 cut(s) 353, 1116, 1334
BaeI ACNNNNGTAYC 2 cut(s) 636, 669
BanI GGYRCC 1 cut(s) 652
BanII GRGCYC 1 cut(s) 1468
BauI CACGAG 1 cut(s) 455
BbsI GAAGAC 1 cut(s) 443
Bbv12I GWGCWC 3 cut(s) 1116, 1349, 1468
BbvI GCAGC 3 cut(s) 58, 553, 1229
BccI CCATC 4 cut(s) 678, 796, 1243, 1426
BciT130I CCWGG 3 cut(s) 377, 561, 920
BciVI GTATCC 1 cut(s) 567
BcnI CCSGG 1 cut(s) 353
BcoDI GTCTC 2 cut(s) 202, 886
BfaI CTAG 1 cut(s) 1040
BfmI CTRYAG 1 cut(s) 1368
BfrI CTTAAG 1 cut(s) 1303
BfuAI ACCTGC 1 cut(s) 290
BfuI GTATCC 1 cut(s) 567
BglI GCCNNNNNGGC 1 cut(s) 1132
BglII AGATCT 1 cut(s) 993
BisI GCNGC 4 cut(s) 72, 542, 1218, 1553
BlsI GCNGC 4 cut(s) 73, 543, 1219, 1554
BmcAI AGTACT 2 cut(s) 1079, 1532
Bme1390I CCNGG 4 cut(s) 353, 377, 561, 920
Bme18I GGWCC 1 cut(s) 714
BmeT110I CYCGRG 2 cut(s) 648, 1461
BmgT120I GGNCC 1 cut(s) 714
BmiI GGNNCC 1 cut(s) 654
BmrFI CCNGG 4 cut(s) 353, 377, 561, 920
BmrI ACTGGG 1 cut(s) 1543
BmsI GCATC 1 cut(s) 1433
BmuI ACTGGG 1 cut(s) 1543
BpiI GAAGAC 1 cut(s) 443
BpuMI CCSGG 1 cut(s) 353
BsaJI CCNNGG 4 cut(s) 389, 631, 749, 1326
BsaWI WCCGGW 2 cut(s) 363, 666
BsaXI ACNNNNNCTCC 2 cut(s) 1429, 1459
Bsc4I CCNNNNNNNGG 2 cut(s) 622, 755
Bse118I RCCGGY 1 cut(s) 607
Bse1I ACTGG 5 cut(s) 830, 902, 1109, 1127, 1538
Bse3DI GCAATG 3 cut(s) 114, 883, 1058
BseBI CCWGG 3 cut(s) 377, 561, 920
BseDI CCNNGG 4 cut(s) 389, 631, 749, 1326
BseGI GGATG 5 cut(s) 369, 520, 612, 807, 915
BseLI CCNNNNNNNGG 2 cut(s) 622, 755
BseMI GCAATG 3 cut(s) 114, 883, 1058
BseMII CTCAG 2 cut(s) 290, 897
BseNI ACTGG 5 cut(s) 830, 902, 1109, 1127, 1538
BseRI GAGGAG 2 cut(s) 1253, 1437
BseSI GKGCMC 3 cut(s) 353, 1116, 1334
BseXI GCAGC 3 cut(s) 58, 553, 1229
BseYI CCCAGC 1 cut(s) 286
BsgI GTGCAG 2 cut(s) 48, 1420
Bsh1285I CGRYCG 1 cut(s) 620
BshFI GGCC 2 cut(s) 125, 1126
BshNI GGYRCC 1 cut(s) 652
BsiEI CGRYCG 1 cut(s) 620
BsiHKAI GWGCWC 3 cut(s) 1116, 1349, 1468
BsiHKCI CYCGRG 2 cut(s) 648, 1461
BsiSI CCGG 4 cut(s) 353, 364, 608, 667
BslFI GGGAC 3 cut(s) 428, 638, 901
BslI CCNNNNNNNGG 2 cut(s) 622, 755
BsmAI GTCTC 2 cut(s) 202, 886
BsmFI GGGAC 3 cut(s) 428, 638, 901
BsmI GAATGC 1 cut(s) 907
BsnI GGCC 2 cut(s) 125, 1126
BsoBI CYCGRG 2 cut(s) 648, 1461
Bsp1286I GDGCHC 5 cut(s) 353, 1116, 1334, 1349, 1468
Bsp143I GATC 7 cut(s) 59, 379, 617, 792, 970, 993, 1151
Bsp19I CCATGG 2 cut(s) 389, 749
BspACI CCGC 1 cut(s) 656
BspANI GGCC 2 cut(s) 125, 1126
BspCNI CTCAG 2 cut(s) 289, 896
BspLI GGNNCC 1 cut(s) 654
BspMAI CTGCAG 1 cut(s) 1372
BspMI ACCTGC 1 cut(s) 290
BspPI GGATC 4 cut(s) 67, 387, 800, 1146
BspT107I GGYRCC 1 cut(s) 652
BspTI CTTAAG 1 cut(s) 1303
BsrDI GCAATG 3 cut(s) 114, 883, 1058
BsrFI RCCGGY 1 cut(s) 607
BsrI ACTGG 5 cut(s) 830, 902, 1109, 1127, 1538
BssAI RCCGGY 1 cut(s) 607
BssECI CCNNGG 4 cut(s) 389, 631, 749, 1326
BssMI GATC 7 cut(s) 59, 379, 617, 792, 970, 993, 1151
BssSI CACGAG 1 cut(s) 455
BssT1I CCWWGG 3 cut(s) 389, 749, 1326
Bst2BI CACGAG 1 cut(s) 455
Bst2UI CCWGG 3 cut(s) 377, 561, 920
Bst4CI ACNGT 4 cut(s) 248, 596, 663, 1518
Bst6I CTCTTC 1 cut(s) 101
BstAFI CTTAAG 1 cut(s) 1303
BstAPI GCANNNNNTGC 2 cut(s) 71, 1111
BstC8I GCNNGC 2 cut(s) 907, 1222
BstDEI CTNAG 4 cut(s) 276, 548, 883, 1548
BstDSI CCRYGG 3 cut(s) 389, 631, 749
BstF5I GGATG 5 cut(s) 369, 520, 612, 807, 915
BstKTI GATC 7 cut(s) 62, 382, 620, 795, 973, 996, 1154
BstMAI GTCTC 2 cut(s) 202, 886
BstMBI GATC 7 cut(s) 59, 379, 617, 792, 970, 993, 1151
BstMCI CGRYCG 1 cut(s) 620
BstMWI GCNNNNNNNGC 5 cut(s) 71, 77, 1111, 1132, 1367
BstNI CCWGG 3 cut(s) 377, 561, 920
BstNSI RCATGY 1 cut(s) 1273
BstSCI CCNGG 4 cut(s) 351, 375, 559, 918
BstSFI CTRYAG 1 cut(s) 1368
BstSLI GKGCMC 3 cut(s) 353, 1116, 1334
BstV1I GCAGC 3 cut(s) 58, 553, 1229
BstV2I GAAGAC 1 cut(s) 443
BstX2I RGATCY 4 cut(s) 59, 379, 993, 1151
BstYI RGATCY 4 cut(s) 59, 379, 993, 1151
BsuI GTATCC 1 cut(s) 567
BsuRI GGCC 2 cut(s) 125, 1126
BtgI CCRYGG 3 cut(s) 389, 631, 749
BtsCI GGATG 5 cut(s) 369, 520, 612, 807, 915
BtsIMutI CAGTG 3 cut(s) 659, 1116, 1523
BveI ACCTGC 1 cut(s) 290
Cac8I GCNNGC 2 cut(s) 907, 1222
CaiI CAGNNNCTG 1 cut(s) 74
Cfr10I RCCGGY 1 cut(s) 607
Cfr13I GGNCC 1 cut(s) 714
Csp6I GTAC 6 cut(s) 490, 653, 988, 1033, 1078, 1531
CviAII CATG 9 cut(s) 385, 390, 418, 586, 750, 1014, 1136, 1270, 1441
CviQI GTAC 6 cut(s) 490, 653, 988, 1033, 1078, 1531
DdeI CTNAG 4 cut(s) 276, 548, 883, 1548
DpnI GATC 7 cut(s) 61, 381, 619, 794, 972, 995, 1153
DpnII GATC 7 cut(s) 59, 379, 617, 792, 970, 993, 1151
DraI TTTAAA 2 cut(s) 54, 211
DrdI GACNNNNNNGTC 1 cut(s) 608
DseDI GACNNNNNNGTC 1 cut(s) 608
EaeI YGGCCR 1 cut(s) 123
Eam1104I CTCTTC 1 cut(s) 101
EarI CTCTTC 1 cut(s) 101
Ecl136II GAGCTC 1 cut(s) 1466
Eco130I CCWWGG 3 cut(s) 389, 749, 1326
Eco24I GRGCYC 1 cut(s) 1468
Eco32I GATATC 1 cut(s) 14
Eco47I GGWCC 1 cut(s) 714
Eco53kI GAGCTC 1 cut(s) 1466
Eco88I CYCGRG 2 cut(s) 648, 1461
EcoICRI GAGCTC 1 cut(s) 1466
EcoRI GAATTC 2 cut(s) 233, 581
EcoRII CCWGG 3 cut(s) 375, 559, 918
EcoRV GATATC 1 cut(s) 14
EcoT14I CCWWGG 3 cut(s) 389, 749, 1326
EcoT22I ATGCAT 2 cut(s) 823, 1015
EcoT38I GRGCYC 1 cut(s) 1468
ErhI CCWWGG 3 cut(s) 389, 749, 1326
FaeI CATG 9 cut(s) 388, 393, 421, 589, 753, 1017, 1139, 1273, 1444
FaqI GGGAC 3 cut(s) 428, 638, 901
FatI CATG 9 cut(s) 384, 389, 417, 585, 749, 1013, 1135, 1269, 1440
FauNDI CATATG 1 cut(s) 527
Fnu4HI GCNGC 4 cut(s) 72, 542, 1218, 1553
FokI GGATG 5 cut(s) 356, 527, 599, 814, 902
FriOI GRGCYC 1 cut(s) 1468
Fsp4HI GCNGC 4 cut(s) 72, 542, 1218, 1553
FspBI CTAG 1 cut(s) 1040
GluI GCNGC 4 cut(s) 72, 542, 1218, 1553
GsaI CCCAGC 1 cut(s) 290
HaeIII GGCC 2 cut(s) 125, 1126
HapII CCGG 4 cut(s) 353, 364, 608, 667
Hin1II CATG 9 cut(s) 388, 393, 421, 589, 753, 1017, 1139, 1273, 1444
HinfI GANTC 8 cut(s) 405, 646, 928, 935, 1049, 1376, 1400, 1414
HpaII CCGG 4 cut(s) 353, 364, 608, 667
HphI GGTGA 2 cut(s) 689, 1371
Hpy166II GTNNAC 1 cut(s) 1114
Hpy188I TCNGA 7 cut(s) 165, 202, 279, 617, 850, 1351, 1429
Hpy188III TCNNGA 4 cut(s) 457, 775, 932, 1235
Hpy8I GTNNAC 1 cut(s) 1114
Hpy99I CGWCG 1 cut(s) 337
HpyAV CCTTC 3 cut(s) 460, 727, 749
HpyCH4III ACNGT 4 cut(s) 248, 596, 663, 1518
HpyF10VI GCNNNNNNNGC 5 cut(s) 71, 77, 1111, 1132, 1367
HpyF3I CTNAG 4 cut(s) 276, 548, 883, 1548
Hsp92II CATG 9 cut(s) 388, 393, 421, 589, 753, 1017, 1139, 1273, 1444
KpnI GGTACC 1 cut(s) 656
Kzo9I GATC 7 cut(s) 59, 379, 617, 792, 970, 993, 1151
LmnI GCTCC 1 cut(s) 1240
Lsp1109I GCAGC 3 cut(s) 58, 553, 1229
LweI GCATC 1 cut(s) 1433
MaeI CTAG 1 cut(s) 1040
MaeIII GTNAC 2 cut(s) 1054, 1377
MalI GATC 7 cut(s) 61, 381, 619, 794, 972, 995, 1153
MboI GATC 7 cut(s) 59, 379, 617, 792, 970, 993, 1151
MboII GAAGA 5 cut(s) 88, 126, 448, 1081, 1535
MfeI CAATTG 1 cut(s) 1206
MflI RGATCY 4 cut(s) 59, 379, 993, 1151
MhlI GDGCHC 5 cut(s) 353, 1116, 1334, 1349, 1468
MluCI AATT 6 cut(s) 157, 233, 581, 1206, 1495, 1500
MlyI GAGTC 3 cut(s) 640, 1385, 1423
MmeI TCCRAC 2 cut(s) 225, 707
MnlI CCTC 9 cut(s) 546, 615, 849, 997, 1091, 1231, 1455, 1458, 1479
Mph1103I ATGCAT 2 cut(s) 823, 1015
MseI TTAA 7 cut(s) 53, 210, 1158, 1283, 1304, 1410, 1494
MslI CAYNNNNRTG 1 cut(s) 1342
MspA1I CMGCKG 1 cut(s) 71
MspCI CTTAAG 1 cut(s) 1303
MspI CCGG 4 cut(s) 353, 364, 608, 667
MspR9I CCNGG 4 cut(s) 353, 377, 561, 920
MunI CAATTG 1 cut(s) 1206
Mva1269I GAATGC 1 cut(s) 907
MvaI CCWGG 3 cut(s) 377, 561, 920
MwoI GCNNNNNNNGC 5 cut(s) 71, 77, 1111, 1132, 1367
NciI CCSGG 1 cut(s) 353
NcoI CCATGG 2 cut(s) 389, 749
NdeI CATATG 1 cut(s) 527
NdeII GATC 7 cut(s) 59, 379, 617, 792, 970, 993, 1151
NlaIII CATG 9 cut(s) 388, 393, 421, 589, 753, 1017, 1139, 1273, 1444
NlaIV GGNNCC 1 cut(s) 654
NmuCI GTSAC 1 cut(s) 1377
NsiI ATGCAT 2 cut(s) 823, 1015
NspI RCATGY 1 cut(s) 1273
OliI CACNNNNGTG 1 cut(s) 1342
PciI ACATGT 1 cut(s) 1269
PctI GAATGC 1 cut(s) 907
PfeI GAWTC 5 cut(s) 405, 928, 935, 1049, 1400
PfoI TCCNGGA 1 cut(s) 918
PkrI GCNGC 4 cut(s) 73, 543, 1219, 1554
Ple19I CGATCG 1 cut(s) 620
PleI GAGTC 3 cut(s) 640, 1384, 1422
PpsI GAGTC 3 cut(s) 640, 1384, 1422
PscI ACATGT 1 cut(s) 1269
PshBI ATTAAT 1 cut(s) 1494
Psp124BI GAGCTC 1 cut(s) 1468
Psp6I CCWGG 3 cut(s) 375, 559, 918
PspFI CCCAGC 1 cut(s) 286
PspGI CCWGG 3 cut(s) 375, 559, 918
PspN4I GGNNCC 1 cut(s) 654
PspPI GGNCC 1 cut(s) 714
PstI CTGCAG 1 cut(s) 1372
PstNI CAGNNNCTG 1 cut(s) 74
PsuI RGATCY 4 cut(s) 59, 379, 993, 1151
PvuI CGATCG 1 cut(s) 620
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 6 cut(s) 491, 654, 989, 1034, 1079, 1532
RsaNI GTAC 6 cut(s) 490, 653, 988, 1033, 1078, 1531
RseI CAYNNNNRTG 1 cut(s) 1342
SacI GAGCTC 1 cut(s) 1468
SaqAI TTAA 7 cut(s) 53, 210, 1158, 1283, 1304, 1410, 1494
SatI GCNGC 4 cut(s) 72, 542, 1218, 1553
Sau3AI GATC 7 cut(s) 59, 379, 617, 792, 970, 993, 1151
Sau96I GGNCC 1 cut(s) 714
ScaI AGTACT 2 cut(s) 1079, 1532
SchI GAGTC 3 cut(s) 640, 1385, 1423
ScrFI CCNGG 4 cut(s) 353, 377, 561, 920
SduI GDGCHC 5 cut(s) 353, 1116, 1334, 1349, 1468
SfaNI GCATC 1 cut(s) 1433
SfcI CTRYAG 1 cut(s) 1368
SinI GGWCC 1 cut(s) 714
SmiMI CAYNNNNRTG 1 cut(s) 1342
SmlI CTYRAG 1 cut(s) 1303
SmoI CTYRAG 1 cut(s) 1303
Sse9I AATT 6 cut(s) 157, 233, 581, 1206, 1495, 1500
SsiI CCGC 1 cut(s) 656
SspI AATATT 1 cut(s) 1492
SspMI CTAG 1 cut(s) 1040
SstI GAGCTC 1 cut(s) 1468
StyD4I CCNGG 4 cut(s) 351, 375, 559, 918
StyI CCWWGG 3 cut(s) 389, 749, 1326
TaaI ACNGT 4 cut(s) 248, 596, 663, 1518
TaqI TCGA 2 cut(s) 620, 644
TasI AATT 6 cut(s) 157, 233, 581, 1206, 1495, 1500
TatI WGTACW 4 cut(s) 489, 1032, 1077, 1530
TfiI GAWTC 5 cut(s) 405, 928, 935, 1049, 1400
Tru1I TTAA 7 cut(s) 53, 210, 1158, 1283, 1304, 1410, 1494
Tru9I TTAA 7 cut(s) 53, 210, 1158, 1283, 1304, 1410, 1494
TscAI CASTG 3 cut(s) 666, 1116, 1523
TseFI GTSAC 1 cut(s) 1377
TseI GCWGC 4 cut(s) 71, 541, 1217, 1552
Tsp45I GTSAC 1 cut(s) 1377
TspGWI ACGGA 1 cut(s) 648
TspRI CASTG 3 cut(s) 666, 1116, 1523
Vha464I CTTAAG 1 cut(s) 1303
VneI GTGCAC 1 cut(s) 1112
VpaK11BI GGWCC 1 cut(s) 714
VspI ATTAAT 1 cut(s) 1494
XapI RAATTY 2 cut(s) 233, 581
XceI RCATGY 1 cut(s) 1273
XspI CTAG 1 cut(s) 1040
ZrmI AGTACT 2 cut(s) 1079, 1532
Zsp2I ATGCAT 2 cut(s) 823, 1015
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.