MD06G1096700.v1.1

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0004902
Physical Location & Seq
Reverse (-)
23184508 .. 23188327
3820 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1096700.v1.1.491

Sequence Viewer

Length: 2274 bp
ATGGCTTCCAATGCTCCAGCAGGGCATACATGTCCGCCTCCAATGAAGGCCACATCAAACGGTGTGTTCCAGGGGGACAGTCCTCTTCATTTTGCACTGCCTCTCGCTATTTTGCAGATATGTATTGTAGTTATAGCTACTCGGGTTCTTGCTTATGTTCTGAAGCCGTTAAGACAGCCACGAGTCATTGCGGAGATTGTTGGAGGAATCCTACTTGGGCCATCAGCTCTGGGACGGAACAAAAGTTATCTTCAGGCAATATTTCCACCGAAGAGTATCACTGTGTTGGACACCTTAGCAAACCTCGGTCTCCTATTCTTTCTATTCCTTGCAGGCGTAGAGATAGATCCTAAATCCATACGTCAGACTGGAAAACAAGCCCTTGCCATTGCCGTAGCAGGAATAACCCTCCCATTTGCATTAGGAATCGGTTCATCATTTGTCCTCCGAGAAACCATAGCCAAAGGGGTAGATGCTACTGCATTTCTTGTGTTCATGGGTGTGGCCCTTTCGATAACTGCTTTCCCTGTTTTACCTCGTATTCTGGCTGAGCTGAAACTCTTGACCACTGACATTGGAAAAATGGCTATGTCAGCTGCCACAGTTAATGATGTGGCTGCCTGGATTCTACTGGCTCTTGCTGTTGCCCTTTCTGGCAGTAACCAATCTCCCCTTGTTTCACTATGGGTCTTCTTGTCTGGGTGTGAACCTGTTGATGAGATCTATGTATGCGCTACACTAACTGCTGTTCTGGCTGCTGGTTTCATTACTGATACAATTGGAATTCATGCCATGTTTGGTGCTTTTGTGGTCGGAGTTCTTGTACCAAAGGAGGGGCCATTTGCAGGTGCTCTTGTGGAAAAAGTAGAAGATCTCATATCTGGTCTCTTTTTACCGGGCTTCAGTTGTGGGGTCTTCTGGTTTTGGTCATATTTACTGCCTGTTTCGGGAAGATTTTTGGGACGATTGTGGTGTCTTTCCTGCAAATTGCCTGTCCGTGAGGCTTTAGCTCTGGGGTTCCTCATGAACAGTAAAGGCTTGATGGAACTCATTGTCCTCAACATCGGTAAAGATAGAAAGGTCTTGAATGACCAGACTTTTGCCATTATGGTTCTAATGGCTCTGTTTACTACTTTTATCACCACGCCCCTTGTTGTTGCCGTGTATAAGCCAGAAAAAAGGGGAAGAATGGCTGATTACAAGCATAAGACAATTGAAAGGAAAAATACGAATACCCAACTAAAGATCTTGGCCTGTTTTCATAGTGCAAGAAACATTCCATCTATAATAAATCTTCTAGAGGCCTCACGAGGCACAAACAAGAGTGACGGCCTTTGTGTCTATGCAATGCACCTCAAGGAGTTCTCTGAGAGGTCATCAGCTATATTAATGGTACACAAGGCGAGAAGAAATGGGTTACCCTTCTGGAATAAGGGCCAGCAATCAAATTCCGACAATGTTGTTGTGGCATTTGAGGCATACCAACAATTAAGCCGGGTGTCAATCAGACCAATGACTGAAATCTCTTCGGTTTTTTATATGCGCGAGGACATTTGTGCCACCGCTGAGAGCAAAAGAGTTGCAATCGTAATTCTTCCATTCCATAAGCACCAGAGGCTAGATGGTACACTAGAGACCACGCGAAATGACTTTCGCTTGGTTAACCAGAAGGTCCTTCAACATGCACCTTGCTCAGTTGGAATTCTGGTTGATCGCGGTCTTGGTGGAGCCACACACATTGCTGCAAGCAACGTTTCTTATTTCATCACAGTTCTCTTCTTTGGGGGTCGTGATGACCGTGAAGCCCTCGCCTATGGTGCTCCTATGGCTGAGCACCCCGGCATTAGTTTAATGGTCATTCACTTCCTAGTGGAACCCGAGGTTGCGGGGGAGTTTTCTAGAATAAATATTGATGACAATGGTGGCACCAAAGCGGGATCAGTAGATGAAGAATTCCTCACTGAGTTCAAGCAGAAAACTGCCATGGATGACTCAATCACATACGAAGAGAAGGTAGTCAGAAATGAGGCGCAAACCATTGCTGTGATTCGTGAGATTGGCCGCTGCAATCTGTTTCTGGTGGGGAGAACACCTTGCGGTGAAGTAGCCTTAGCTTTAAATAGGAGGAGTGAGTGGCCCGAACTTGGTCCTGTTGGTAGCTTGTTAATTTCACCAGGTTTTCCTACACCAGCATCAGTCTTGGTAGTGCAACAGTACAATTGTCAGGTATCGTTGAATTTGACCTCGGAGATGGAGGAAGAGTTACCTGAATGA

Protein Analysis

758

Amino Acids

82.27

Weight (kDa)

7.87

Isoelectric Point (pI)

41.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 47 - 230 8.5e-29 Sodium/hydrogen exchanger, transmembrane
Na_H_Exchanger PF00999 236 - 384 8.6e-13 Sodium/hydrogen exchanger, transmembrane
CHX17_2nd PF23256 445 - 584 6e-43 Plant cation/H(+) antiporter domain
CHX17_C PF23259 588 - 740 4.7e-28 Plant cation/H(+) antiporter, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 836
Acc36I ACCTGC 1 cut(s) 836
AccB1I GGYRCC 1 cut(s) 1925
AccII CGCG 3 cut(s) 1545, 1642, 1716
AciI CCGC 8 cut(s) 35, 191, 1563, 1716, 1886, 1934, 2062, 2097
AclI AACGTT 1 cut(s) 1752
AclWI GGATC 2 cut(s) 341, 1945
AcoI YGGCCR 1 cut(s) 2059
AcsI RAATTY 5 cut(s) 783, 1447, 1701, 1952, 2236
AcuI CTGAAG 3 cut(s) 182, 236, 886
AfaI GTAC 4 cut(s) 825, 1395, 1627, 2216
AfiI CCNNNNNNNGG 4 cut(s) 833, 845, 947, 2144
AflIII ACRYGT 1 cut(s) 29
AgsI TTSAA 5 cut(s) 1087, 1217, 1679, 1969, 2236
AjnI CCWGG 3 cut(s) 69, 620, 2173
AloI GAACNNNNNNTCC 2 cut(s) 1038, 1070
AluBI AGCT 8 cut(s) 137, 227, 553, 596, 1010, 1382, 2114, 2160
AluI AGCT 8 cut(s) 137, 227, 553, 596, 1010, 1382, 2114, 2160
Alw21I GWGCWC 3 cut(s) 853, 1822, 1836
Alw26I GTCTC 3 cut(s) 314, 890, 1628
AlwI GGATC 2 cut(s) 341, 1945
Ama87I CYCGRG 2 cut(s) 141, 1877
ApeKI GCWGC 5 cut(s) 596, 617, 755, 1742, 2064
ApoI RAATTY 5 cut(s) 783, 1447, 1701, 1952, 2236
ArsI GACNNNNNNTTYG 4 cut(s) 978, 1010, 1082, 1114
AseI ATTAAT 1 cut(s) 1388
Asp700I GAANNNNTTC 1 cut(s) 430
AspLEI GCGC 3 cut(s) 734, 1545, 2032
AspS9I GGNCC 7 cut(s) 218, 505, 836, 1435, 1672, 2136, 2147
AsuC2I CCSGG 3 cut(s) 897, 1496, 1839
AsuHPI GGTGA 3 cut(s) 1132, 2111, 2163
AvaI CYCGRG 2 cut(s) 141, 1877
AvaII GGWCC 2 cut(s) 1672, 2147
BanI GGYRCC 1 cut(s) 1925
BauI CACGAG 2 cut(s) 180, 1308
BbsI GAAGAC 2 cut(s) 682, 907
Bbv12I GWGCWC 3 cut(s) 853, 1822, 1836
BbvI GCAGC 5 cut(s) 583, 604, 742, 1729, 2051
BccI CCATC 5 cut(s) 229, 1036, 1288, 1616, 2245
BceAI ACGGC 4 cut(s) 151, 377, 1145, 1345
BciT130I CCWGG 3 cut(s) 71, 622, 2175
BcnI CCSGG 3 cut(s) 897, 1496, 1839
BcoDI GTCTC 3 cut(s) 314, 890, 1628
BfaI CTAG 5 cut(s) 1298, 1619, 1631, 1868, 1899
BfuAI ACCTGC 1 cut(s) 836
BglII AGATCT 3 cut(s) 720, 871, 1245
BisI GCNGC 6 cut(s) 597, 618, 756, 1743, 2062, 2065
BlpI GCTNAGC 2 cut(s) 549, 1830
BlsI GCNGC 6 cut(s) 598, 619, 757, 1744, 2063, 2066
Bme1390I CCNGG 6 cut(s) 71, 622, 897, 1496, 1839, 2175
Bme18I GGWCC 2 cut(s) 1672, 2147
BmeT110I CYCGRG 2 cut(s) 141, 1877
BmgT120I GGNCC 7 cut(s) 218, 505, 836, 1435, 1672, 2136, 2147
BmiI GGNNCC 5 cut(s) 837, 1019, 1729, 1875, 1927
BmrFI CCNGG 6 cut(s) 71, 622, 897, 1496, 1839, 2175
BmsI GCATC 2 cut(s) 463, 2201
BpiI GAAGAC 2 cut(s) 682, 907
Bpu10I CCTNAGC 2 cut(s) 295, 2110
Bpu1102I GCTNAGC 2 cut(s) 549, 1830
BpuEI CTTGAG 1 cut(s) 1340
BpuMI CCSGG 3 cut(s) 897, 1496, 1839
BsaI GGTCTC 3 cut(s) 314, 890, 1628
BsaJI CCNNGG 6 cut(s) 70, 304, 1837, 1878, 1983, 2244
BsaXI ACNNNNNCTCC 3 cut(s) 807, 837, 2246
Bsc4I CCNNNNNNNGG 4 cut(s) 833, 845, 947, 2144
Bse1I ACTGG 2 cut(s) 373, 636
Bse3DI GCAATG 5 cut(s) 186, 387, 1353, 1737, 2037
BseBI CCWGG 3 cut(s) 71, 622, 2175
BseDI CCNNGG 6 cut(s) 70, 304, 1837, 1878, 1983, 2244
BseGI GGATG 1 cut(s) 1993
BseLI CCNNNNNNNGG 4 cut(s) 833, 845, 947, 2144
BseMI GCAATG 5 cut(s) 186, 387, 1353, 1737, 2037
BseMII CTCAG 6 cut(s) 540, 1359, 1557, 1707, 1821, 1953
BseNI ACTGG 2 cut(s) 373, 636
BseRI GAGGAG 1 cut(s) 2140
BseXI GCAGC 5 cut(s) 583, 604, 742, 1729, 2051
Bsh1236I CGCG 3 cut(s) 1545, 1642, 1716
BshNI GGYRCC 1 cut(s) 1925
BsiHKAI GWGCWC 3 cut(s) 853, 1822, 1836
BsiHKCI CYCGRG 2 cut(s) 141, 1877
BsiSI CCGG 3 cut(s) 896, 1495, 1839
BslFI GGGAC 3 cut(s) 89, 246, 975
BslI CCNNNNNNNGG 4 cut(s) 833, 845, 947, 2144
BsmAI GTCTC 3 cut(s) 314, 890, 1628
BsmFI GGGAC 3 cut(s) 89, 246, 975
Bso31I GGTCTC 3 cut(s) 314, 890, 1628
BsoBI CYCGRG 2 cut(s) 141, 1877
Bsp1286I GDGCHC 3 cut(s) 853, 1822, 1836
Bsp143I GATC 6 cut(s) 346, 720, 871, 1245, 1711, 1937
Bsp1720I GCTNAGC 2 cut(s) 549, 1830
Bsp19I CCATGG 1 cut(s) 1983
BspACI CCGC 8 cut(s) 35, 191, 1563, 1716, 1886, 1934, 2062, 2097
BspCNI CTCAG 6 cut(s) 541, 1360, 1558, 1706, 1822, 1954
BspFNI CGCG 3 cut(s) 1545, 1642, 1716
BspHI TCATGA 1 cut(s) 1023
BspLI GGNNCC 5 cut(s) 837, 1019, 1729, 1875, 1927
BspMI ACCTGC 1 cut(s) 836
BspPI GGATC 2 cut(s) 341, 1945
BspT107I GGYRCC 1 cut(s) 1925
BspTNI GGTCTC 3 cut(s) 314, 890, 1628
BsrDI GCAATG 5 cut(s) 186, 387, 1353, 1737, 2037
BsrI ACTGG 2 cut(s) 373, 636
BssECI CCNNGG 6 cut(s) 70, 304, 1837, 1878, 1983, 2244
BssMI GATC 6 cut(s) 346, 720, 871, 1245, 1711, 1937
BssSI CACGAG 2 cut(s) 180, 1308
BssT1I CCWWGG 1 cut(s) 1983
Bst2BI CACGAG 2 cut(s) 180, 1308
Bst2UI CCWGG 3 cut(s) 71, 622, 2175
Bst4CI ACNGT 8 cut(s) 62, 80, 283, 604, 1031, 1771, 1799, 2214
Bst6I CTCTTC 6 cut(s) 90, 266, 1531, 1781, 2001, 2253
BstC8I GCNNGC 3 cut(s) 334, 1439, 1747
BstDEI CTNAG 8 cut(s) 295, 549, 1368, 1566, 1693, 1830, 1962, 2110
BstDSI CCRYGG 1 cut(s) 1983
BstEII GGTNACC 1 cut(s) 1416
BstF5I GGATG 1 cut(s) 1993
BstFNI CGCG 3 cut(s) 1545, 1642, 1716
BstHHI GCGC 3 cut(s) 734, 1545, 2032
BstKTI GATC 6 cut(s) 349, 723, 874, 1248, 1714, 1940
BstMAI GTCTC 3 cut(s) 314, 890, 1628
BstMBI GATC 6 cut(s) 346, 720, 871, 1245, 1711, 1937
BstMWI GCNNNNNNNGC 7 cut(s) 11, 593, 752, 1475, 1615, 1817, 1826
BstNI CCWGG 3 cut(s) 71, 622, 2175
BstNSI RCATGY 2 cut(s) 33, 1685
BstPI GGTNACC 1 cut(s) 1416
BstSCI CCNGG 6 cut(s) 69, 620, 895, 1494, 1837, 2173
BstUI CGCG 3 cut(s) 1545, 1642, 1716
BstV1I GCAGC 5 cut(s) 583, 604, 742, 1729, 2051
BstV2I GAAGAC 2 cut(s) 682, 907
BstX2I RGATCY 4 cut(s) 346, 720, 871, 1245
BstYI RGATCY 4 cut(s) 346, 720, 871, 1245
BtgI CCRYGG 1 cut(s) 1983
BtsCI GGATG 1 cut(s) 1993
BtsI GCAGTG 1 cut(s) 95
BtsIMutI CAGTG 4 cut(s) 95, 279, 567, 1959
BveI ACCTGC 1 cut(s) 836
Cac8I GCNNGC 3 cut(s) 334, 1439, 1747
CciI TCATGA 1 cut(s) 1023
CfoI GCGC 3 cut(s) 734, 1545, 2032
Cfr13I GGNCC 7 cut(s) 218, 505, 836, 1435, 1672, 2136, 2147
CsiI ACCWGGT 1 cut(s) 2173
Csp6I GTAC 4 cut(s) 824, 1394, 1626, 2215
CspCI CAANNNNNGTGG 6 cut(s) 556, 591, 1132, 1167, 1720, 1755
CviAII CATG 7 cut(s) 30, 496, 788, 793, 1024, 1682, 1984
CviQI GTAC 4 cut(s) 824, 1394, 1626, 2215
DdeI CTNAG 8 cut(s) 295, 549, 1368, 1566, 1693, 1830, 1962, 2110
DpnI GATC 6 cut(s) 348, 722, 873, 1247, 1713, 1939
DpnII GATC 6 cut(s) 346, 720, 871, 1245, 1711, 1937
DraI TTTAAA 1 cut(s) 2118
EaeI YGGCCR 1 cut(s) 2059
Eam1104I CTCTTC 6 cut(s) 90, 266, 1531, 1781, 2001, 2253
EarI CTCTTC 6 cut(s) 90, 266, 1531, 1781, 2001, 2253
EciI GGCGGA 1 cut(s) 24
Eco130I CCWWGG 1 cut(s) 1983
Eco147I AGGCCT 1 cut(s) 1304
Eco31I GGTCTC 3 cut(s) 314, 890, 1628
Eco47I GGWCC 2 cut(s) 1672, 2147
Eco57I CTGAAG 3 cut(s) 182, 236, 886
Eco88I CYCGRG 2 cut(s) 141, 1877
Eco91I GGTNACC 1 cut(s) 1416
EcoO109I RGGNCCY 1 cut(s) 1672
EcoO65I GGTNACC 1 cut(s) 1416
EcoRI GAATTC 3 cut(s) 783, 1701, 1952
EcoRII CCWGG 3 cut(s) 69, 620, 2173
EcoT14I CCWWGG 1 cut(s) 1983
ErhI CCWWGG 1 cut(s) 1983
FaeI CATG 7 cut(s) 33, 499, 791, 796, 1027, 1685, 1987
FaqI GGGAC 3 cut(s) 89, 246, 975
FatI CATG 7 cut(s) 29, 495, 787, 792, 1023, 1681, 1983
FauI CCCGC 2 cut(s) 1879, 1927
Fnu4HI GCNGC 6 cut(s) 597, 618, 756, 1743, 2062, 2065
FokI GGATG 1 cut(s) 2000
Fsp4HI GCNGC 6 cut(s) 597, 618, 756, 1743, 2062, 2065
FspBI CTAG 5 cut(s) 1298, 1619, 1631, 1868, 1899
GlaI GCGC 3 cut(s) 733, 1544, 2031
GluI GCNGC 6 cut(s) 597, 618, 756, 1743, 2062, 2065
HapII CCGG 3 cut(s) 896, 1495, 1839
HhaI GCGC 3 cut(s) 734, 1545, 2032
Hin1II CATG 7 cut(s) 33, 499, 791, 796, 1027, 1685, 1987
Hin6I GCGC 3 cut(s) 732, 1543, 2030
HinP1I GCGC 3 cut(s) 732, 1543, 2030
HincII GTYRAC 1 cut(s) 1663
HindII GTYRAC 1 cut(s) 1663
HinfI GANTC 6 cut(s) 183, 207, 426, 625, 1991, 2047
HpaI GTTAAC 1 cut(s) 1663
HpaII CCGG 3 cut(s) 896, 1495, 1839
HphI GGTGA 3 cut(s) 1132, 2111, 2163
Hpy166II GTNNAC 5 cut(s) 707, 1128, 1396, 1628, 1663
Hpy188I TCNGA 9 cut(s) 162, 366, 449, 815, 1369, 1453, 1508, 2021, 2248
Hpy8I GTNNAC 5 cut(s) 707, 1128, 1396, 1628, 1663
HpyAV CCTTC 5 cut(s) 40, 1432, 1663, 1685, 2005
HpyCH4III ACNGT 8 cut(s) 62, 80, 283, 604, 1031, 1771, 1799, 2214
HpyCH4IV ACGT 2 cut(s) 361, 1752
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 593, 752, 1475, 1615, 1817, 1826
HpyF3I CTNAG 8 cut(s) 295, 549, 1368, 1566, 1693, 1830, 1962, 2110
HpySE526I ACGT 2 cut(s) 361, 1752
Hsp92II CATG 7 cut(s) 33, 499, 791, 796, 1027, 1685, 1987
HspAI GCGC 3 cut(s) 732, 1543, 2030
KspAI GTTAAC 1 cut(s) 1663
Kzo9I GATC 6 cut(s) 346, 720, 871, 1245, 1711, 1937
LmnI GCTCC 3 cut(s) 19, 1727, 1825
Lsp1109I GCAGC 5 cut(s) 583, 604, 742, 1729, 2051
LweI GCATC 2 cut(s) 463, 2201
MabI ACCWGGT 1 cut(s) 2173
MaeI CTAG 5 cut(s) 1298, 1619, 1631, 1868, 1899
MaeII ACGT 2 cut(s) 361, 1752
MaeIII GTNAC 4 cut(s) 659, 1325, 1416, 2262
MalI GATC 6 cut(s) 348, 722, 873, 1247, 1713, 1939
MboI GATC 6 cut(s) 346, 720, 871, 1245, 1711, 1937
MfeI CAATTG 3 cut(s) 777, 1212, 2218
MflI RGATCY 4 cut(s) 346, 720, 871, 1245
MhlI GDGCHC 3 cut(s) 853, 1822, 1836
MlyI GAGTC 2 cut(s) 192, 1985
MmeI TCCRAC 5 cut(s) 181, 267, 793, 1476, 1678
MroXI GAANNNNTTC 1 cut(s) 430
MseI TTAA 8 cut(s) 170, 606, 1388, 1490, 1662, 1850, 2117, 2165
MslI CAYNNNNRTG 2 cut(s) 500, 2042
MspA1I CMGCKG 3 cut(s) 596, 1565, 2064
MspI CCGG 3 cut(s) 896, 1495, 1839
MspR9I CCNGG 6 cut(s) 71, 622, 897, 1496, 1839, 2175
MunI CAATTG 3 cut(s) 777, 1212, 2218
MvaI CCWGG 3 cut(s) 71, 622, 2175
MvnI CGCG 3 cut(s) 1545, 1642, 1716
MwoI GCNNNNNNNGC 7 cut(s) 11, 593, 752, 1475, 1615, 1817, 1826
NciI CCSGG 3 cut(s) 897, 1496, 1839
NcoI CCATGG 1 cut(s) 1983
NdeII GATC 6 cut(s) 346, 720, 871, 1245, 1711, 1937
NlaIII CATG 7 cut(s) 33, 499, 791, 796, 1027, 1685, 1987
NlaIV GGNNCC 5 cut(s) 837, 1019, 1729, 1875, 1927
NmuCI GTSAC 1 cut(s) 1325
NspI RCATGY 2 cut(s) 33, 1685
PagI TCATGA 1 cut(s) 1023
PaqCI CACCTGC 1 cut(s) 836
PceI AGGCCT 1 cut(s) 1304
PciI ACATGT 1 cut(s) 29
PcsI WCGNNNNNNNCGW 1 cut(s) 1795
PdmI GAANNNNTTC 1 cut(s) 430
PfeI GAWTC 4 cut(s) 207, 426, 625, 2047
PkrI GCNGC 6 cut(s) 598, 619, 757, 1744, 2063, 2066
PleI GAGTC 2 cut(s) 191, 1985
PpsI GAGTC 2 cut(s) 191, 1985
PpuMI RGGWCCY 1 cut(s) 1672
PscI ACATGT 1 cut(s) 29
PshBI ATTAAT 1 cut(s) 1388
Psp1406I AACGTT 1 cut(s) 1752
Psp5II RGGWCCY 1 cut(s) 1672
Psp6I CCWGG 3 cut(s) 69, 620, 2173
PspEI GGTNACC 1 cut(s) 1416
PspGI CCWGG 3 cut(s) 69, 620, 2173
PspN4I GGNNCC 5 cut(s) 837, 1019, 1729, 1875, 1927
PspPI GGNCC 7 cut(s) 218, 505, 836, 1435, 1672, 2136, 2147
PspPPI RGGWCCY 1 cut(s) 1672
PsuI RGATCY 4 cut(s) 346, 720, 871, 1245
PvuII CAGCTG 1 cut(s) 596
RsaI GTAC 4 cut(s) 825, 1395, 1627, 2216
RsaNI GTAC 4 cut(s) 824, 1394, 1626, 2215
RseI CAYNNNNRTG 2 cut(s) 500, 2042
SaqAI TTAA 8 cut(s) 170, 606, 1388, 1490, 1662, 1850, 2117, 2165
SatI GCNGC 6 cut(s) 597, 618, 756, 1743, 2062, 2065
Sau3AI GATC 6 cut(s) 346, 720, 871, 1245, 1711, 1937
Sau96I GGNCC 7 cut(s) 218, 505, 836, 1435, 1672, 2136, 2147
SchI GAGTC 2 cut(s) 192, 1985
ScrFI CCNGG 6 cut(s) 71, 622, 897, 1496, 1839, 2175
SduI GDGCHC 3 cut(s) 853, 1822, 1836
SexAI ACCWGGT 1 cut(s) 2173
SfaNI GCATC 2 cut(s) 463, 2201
SinI GGWCC 2 cut(s) 1672, 2147
SmiMI CAYNNNNRTG 2 cut(s) 500, 2042
SmlI CTYRAG 1 cut(s) 1355
SmoI CTYRAG 1 cut(s) 1355
SseBI AGGCCT 1 cut(s) 1304
SsiI CCGC 8 cut(s) 35, 191, 1563, 1716, 1886, 1934, 2062, 2097
SspI AATATT 2 cut(s) 261, 1909
SspMI CTAG 5 cut(s) 1298, 1619, 1631, 1868, 1899
StuI AGGCCT 1 cut(s) 1304
StyD4I CCNGG 6 cut(s) 69, 620, 895, 1494, 1837, 2173
StyI CCWWGG 1 cut(s) 1983
TaaI ACNGT 8 cut(s) 62, 80, 283, 604, 1031, 1771, 1799, 2214
TaiI ACGT 2 cut(s) 364, 1755
TaqI TCGA 1 cut(s) 512
TaqII GACCGA 1 cut(s) 296
TatI WGTACW 1 cut(s) 2214
TauI GCSGC 1 cut(s) 2064
TfiI GAWTC 4 cut(s) 207, 426, 625, 2047
Tru1I TTAA 8 cut(s) 170, 606, 1388, 1490, 1662, 1850, 2117, 2165
Tru9I TTAA 8 cut(s) 170, 606, 1388, 1490, 1662, 1850, 2117, 2165
TscAI CASTG 4 cut(s) 102, 286, 574, 1966
TseFI GTSAC 1 cut(s) 1325
TseI GCWGC 5 cut(s) 596, 617, 755, 1742, 2064
Tsp45I GTSAC 1 cut(s) 1325
TspGWI ACGGA 2 cut(s) 250, 986
TspRI CASTG 4 cut(s) 102, 286, 574, 1966
VpaK11BI GGWCC 2 cut(s) 1672, 2147
VspI ATTAAT 1 cut(s) 1388
XapI RAATTY 5 cut(s) 783, 1447, 1701, 1952, 2236
XbaI TCTAGA 2 cut(s) 1297, 1898
XceI RCATGY 2 cut(s) 33, 1685
XcmI CCANNNNNNNNNTGG 1 cut(s) 1619
XmnI GAANNNNTTC 1 cut(s) 430
XspI CTAG 5 cut(s) 1298, 1619, 1631, 1868, 1899
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.