Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0008311

Physical Location & Seq

Genomic Coordinates Forward (+)

23781673 .. 23785099

Gene Structure 3427 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1101300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2244 bp

ATGGAACAATTCCGGCATATTGGAGAGGTTTTGGGAAGTATGAAGGCTCTGATGATGTTGAGAGATGAGATTCTGATCAATCAATGCCAGTGCTGTTTGTTGCATGATGTGTTCACGTTGGCGTTTGACACGATCGGAGAGGAGATCCAGTTGAACCTAAAACTCGAAGAGAAGAAGACGAAATGGAAAGCCCTCGAGCAGCCTCTGAGGGAGCTGCACAGGGTTTTCAAGGAAGGGGAGCTTTATATGCGGCATTGCATGGATGTGAAAAACTGGTGGGGCAAAACAATCCTCCTCCATCAGAACAAGGACTGCGTCGAATTTCACATCCACAACTTGTTCTGCCACTATGCAGCCGTGATTGAGGCGATTGAGAATGCTGGAGAGATTGCGGGGCTCGATCAGGAGGAGATGCACAAGAAGAGGGTTCTGCTCACGAGGAAGTATGACAGGGAGTGGAATGACCCCAAACTTTTCCAATGGAGATTTGGGAAGCAGTATTTGGTCCCGAAGTTGATTTGCAACAGGTTGGAAAGTGCTTGGAGGGAAGATAGATGGCGGCTTGTTGAAGCTCTTAAGGTGAAAAAGATTTCAGGATCCTTTGGTTCAACGAGGAATGAGCAGCAGCTTGGAGACTTGCTGCTCAAGAAACTGAGTGGGTCGGAAACCTTAAATGGGAAGCTCTTTCCGAGCTCAATCTTATTGGGATCAGCGGATTACCATGTTAGGCGGCGGTTAGGAGGGGGAACGCAGTACAAGGAGATCCAGTGGTTGGGCCAGAACTTTGCCATGAGACACTTCTTTGGGGATATTGAACCTTTGAATTCGGAGATTTCAACTCTTCTCACACTTTCCCACCCCAATGTGCTGCAGTACCTTTCCGGGTTTTATGACGAGGAAAAGAAGGAATGCTTTCTTGTTATGGAGTTGATGAACAAGGATCTGCGCTGCTACATGAAGGAAAACTGTGGCGCAAGAAGGCAGGTTTTGTTTTCACTCCCGGTAGTGGTTGATATCATGCTTCAGATTGCAAGAGGCATGGAATATCTCCACTCTAGGAAGATCTACCATGGGGAGTTGAACCCTTTGAATGTCTTTCTTAAGGCAAGGAGCTGCACAGAAGGCTATTTTCATGTAAAAGTCTCAGGTTTTGGTTTATCATGCGTGCGCAAACCTACTTATCGAAAATCACAGCAGCAGAACGAAATCAACCCTTTGATTTGGTGTGCCCCGGAAGTCCTAGCTGAGCAAGAGCAACCAGGAAACAAAGGTCGTTCCAAATACACGGAGAAAGCAGACGTATACAGCTTCGCGATGCTTTGCTTTGAGCTCTTGACTGGGAAAGTTCCTTTCGAAGATTCACATCTTCAAGGGGACAAGATGAGTAACACTATAAGAGCAGGAGGGAGGCCTCTCTTCCCATACCCTTCACCAAAATACCTTGTCAATTTAACCAAGAGATGCTGGCACAGTGACCCGTCTCAGCGCCTGAGCTTCTCATCCATCTGTCGCGTTTTGCGCTACATAAAGAAGTTCCTTACTCTGAACCCCGACGACGATCAGCCTATACTGCAATCCCCTCCTACGGATTACTGCGAGATAGAGTCATGGTTCCTGAAGAATAGTTCAGCTGCCGGGTACGCTGATTTATCCTCAATATCGCAACTTCCATTCCAAATGTTCTCTTACCGGCTTGGGGAGAAGGAGAAGACTAGCCCTGGCATCATGAGAATCAGGAGTCTGGATTCCATAAGTGATACAACATCCGCAAATTTCAAGCAAGAATCTCCGAATGGCAACGTTGACACTATGTCCATTGCAGAAGATCCATTCGTACCACTAAGCGAAACGAGGTCCGTCTGTTTTGATGTGAGGTCCTCTTATGATCTAAGGTCAGTTTGTTCCGAGGCTCCAATGAAGAGAACTCTCACTGCGAAGAAACGTCAAGAGGCGATGGCTAGAAAAGCCTCAGGTTACACACGGACATCAAAAACACCAACAACACCAAGACTCTCAACGCCAAGGCTTCCGTCGCCAAGATTTCCATCCCCAAAACTTCCGCCACCAAAACTCGCTTCACCGAAACTTCCACCAGCAAAGCTTTCGGTTCCAGCAAAACCATGTCTACGCGGTATGAAGACAAACCAAAGCCCTCCACAGCCGAGTCCTATGAGCACAAAGAGTAGTGCGAGCGGAAGCAGGCACAGAACGGGCGGACACGTCTCGGATTCGGAGATACATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF1221 Pkinase_Tyr

Protein Analysis

748

Amino Acids

85.5

Weight (kDa)

9.19

Isoelectric Point (pI)

60.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF1221	PF06760	21 - 237	6.8e-101	Protein of unknown function (DUF1221)
PK_Tyr_Ser-Thr	PF07714	274 - 505	8.3e-39	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	274 - 503	3.1e-32	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008311)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G64300 AT1G64300 AT5G41730 AT5G41730
fragaria_vesca	FvH4_5g03980 FvH4_5g03980 FvH4_5g03980
malus_domestica	MD06G1101300.v1.1 MD14G1121000.v1.1
prunus_persica	Prupe.5G114700_v2.0.a1 Prupe.5G114700_v2.0.a1
pyrus_communis	pycom06g09650
rosa_chinensis	RchiOBHm_Chr7g0195511
rosa_laevigata	RLG00000004113
rosa_multiflora	Rmu_sc0007727.1_g000023 Rmu_ssc0000078.1_g000002
rosa_rugosa	Rorug07G0023900
rosa_samantha	Rh7AG151500 Rh7BG153200 Rh7CG156700 Rh7DG152500
rosa_wichuraiana	Rw2G030980 Rw7G012940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1169
Acc36I	ACCTGC	1 cut(s)	973
AccB7I	CCANNNNNTGG	1 cut(s)	772
AccBSI	CCGCTC	1 cut(s)	2193
AccI	GTMKAC	2 cut(s)	1302, 2125
AccII	CGCG	3 cut(s)	1313, 1512, 2130
AclI	AACGTT	1 cut(s)	1800
AclWI	GGATC	7 cut(s)	139, 591, 604, 715, 757, 948, 1820
AcsI	RAATTY	3 cut(s)	320, 823, 1771
AcuI	CTGAAG	2 cut(s)	1007, 1637
AfaI	GTAC	4 cut(s)	755, 875, 1640, 1836
AfiI	CCNNNNNNNGG	5 cut(s)	675, 772, 1006, 1585, 1696
AflII	CTTAAG	2 cut(s)	575, 1100
AflIII	ACRYGT	1 cut(s)	2218
AhdI	GACNNNNNGTC	1 cut(s)	1810
AjiI	CACGTC	1 cut(s)	2221
AjnI	CCWGG	2 cut(s)	1258, 1717
AjuI	GAANNNNNNNTTGG	4 cut(s)	485, 517, 2059, 2091
Alw21I	GWGCWC	3 cut(s)	695, 1332, 2177
Alw26I	GTCTC	5 cut(s)	627, 787, 1147, 1485, 2227
AlwI	GGATC	7 cut(s)	139, 591, 604, 715, 757, 948, 1820
AlwNI	CAGNNNCTG	2 cut(s)	205, 1489
Ama87I	CYCGRG	1 cut(s)	194
AoxI	GGCC	2 cut(s)	775, 1409
ApoI	RAATTY	3 cut(s)	320, 823, 1771
Asp700I	GAANNNNTTC	1 cut(s)	912
AspLEI	GCGC	5 cut(s)	948, 974, 1170, 1488, 1521
AspS9I	GGNCC	4 cut(s)	505, 775, 1854, 1875
AsuC2I	CCSGG	4 cut(s)	883, 1001, 1232, 1636
AsuHPI	GGTGA	3 cut(s)	592, 1422, 2070
AsuII	TTCGAA	1 cut(s)	1353
AvaI	CYCGRG	1 cut(s)	194
AvaII	GGWCC	3 cut(s)	505, 1854, 1875
AxyI	CCTNAGG	1 cut(s)	1969
BaeGI	GKGCMC	1 cut(s)	1231
BamHI	GGATCC	1 cut(s)	596
BanII	GRGCYC	3 cut(s)	399, 695, 1332
BauI	CACGAG	1 cut(s)	436
BbsI	GAAGAC	3 cut(s)	182, 1715, 2144
Bbv12I	GWGCWC	3 cut(s)	695, 1332, 2177
BccI	CCATC	5 cut(s)	306, 549, 1511, 1948, 2053
BceAI	ACGGC	1 cut(s)	341
BcgI	CGANNNNNNTGC	2 cut(s)	2085, 2119
BciT130I	CCWGG	2 cut(s)	1260, 1719
BclI	TGATCA	1 cut(s)	75
BcnI	CCSGG	4 cut(s)	883, 1001, 1232, 1636
BcoDI	GTCTC	5 cut(s)	627, 787, 1147, 1485, 2227
BfaI	CTAG	4 cut(s)	1056, 1241, 1713, 1959
BfmI	CTRYAG	1 cut(s)	869
BfoI	RGCGCY	1 cut(s)	1489
BfrI	CTTAAG	2 cut(s)	575, 1100
BfuAI	ACCTGC	1 cut(s)	973
BglII	AGATCT	1 cut(s)	1062
BlpI	GCTNAGC	1 cut(s)	1245
Bme1390I	CCNGG	6 cut(s)	883, 1001, 1232, 1260, 1636, 1719
Bme18I	GGWCC	3 cut(s)	505, 1854, 1875
BmeRI	GACNNNNNGTC	1 cut(s)	1810
BmeT110I	CYCGRG	1 cut(s)	194
BmgBI	CACGTC	1 cut(s)	2221
BmgT120I	GGNCC	4 cut(s)	505, 775, 1854, 1875
BmiI	GGNNCC	5 cut(s)	507, 598, 1613, 1911, 2109
BmrFI	CCNGG	6 cut(s)	883, 1001, 1232, 1260, 1636, 1719
BmrI	ACTGGG	1 cut(s)	1347
BmsI	GCATC	4 cut(s)	402, 1305, 1451, 1731
BmuI	ACTGGG	1 cut(s)	1347
BpiI	GAAGAC	3 cut(s)	182, 1715, 2144
BpmI	CTGGAG	1 cut(s)	402
Bpu10I	CCTNAGC	1 cut(s)	1490
Bpu1102I	GCTNAGC	1 cut(s)	1245
Bpu14I	TTCGAA	1 cut(s)	1353
BpuEI	CTTGAG	1 cut(s)	629
BpuMI	CCSGG	4 cut(s)	883, 1001, 1232, 1636
BsaBI	GATNNNNATC	3 cut(s)	74, 1362, 2044
BsaJI	CCNNGG	5 cut(s)	1069, 1230, 1717, 1905, 2021
BsaXI	ACNNNNNCTCC	4 cut(s)	134, 164, 752, 782
Bsc4I	CCNNNNNNNGG	5 cut(s)	675, 772, 1006, 1585, 1696
Bse118I	RCCGGY	1 cut(s)	1689
Bse1I	ACTGG	5 cut(s)	88, 148, 278, 766, 1342
Bse21I	CCTNAGG	1 cut(s)	1969
Bse3DI	GCAATG	2 cut(s)	253, 1815
Bse8I	GATNNNNATC	3 cut(s)	74, 1362, 2044
BseBI	CCWGG	2 cut(s)	1260, 1719
BseDI	CCNNGG	5 cut(s)	1069, 1230, 1717, 1905, 2021
BseGI	GGATG	5 cut(s)	268, 327, 1499, 1763, 2045
BseJI	GATNNNNATC	3 cut(s)	74, 1362, 2044
BseLI	CCNNNNNNNGG	5 cut(s)	675, 772, 1006, 1585, 1696
BseMI	GCAATG	2 cut(s)	253, 1815
BseMII	CTCAG	7 cut(s)	197, 644, 1158, 1236, 1481, 1496, 1983
BseNI	ACTGG	5 cut(s)	88, 148, 278, 766, 1342
BseRI	GAGGAG	3 cut(s)	155, 284, 422
BseSI	GKGCMC	1 cut(s)	1231
BsgI	GTGCAG	2 cut(s)	200, 1099
Bsh1236I	CGCG	3 cut(s)	1313, 1512, 2130
Bsh1285I	CGRYCG	1 cut(s)	135
BshFI	GGCC	2 cut(s)	777, 1411
BsiEI	CGRYCG	1 cut(s)	135
BsiHKAI	GWGCWC	3 cut(s)	695, 1332, 2177
BsiHKCI	CYCGRG	1 cut(s)	194
BsiSI	CCGG	6 cut(s)	13, 882, 1001, 1232, 1635, 1690
BslFI	GGGAC	2 cut(s)	491, 1388
BslI	CCNNNNNNNGG	5 cut(s)	675, 772, 1006, 1585, 1696
BsmAI	GTCTC	5 cut(s)	627, 787, 1147, 1485, 2227
BsmBI	CGTCTC	2 cut(s)	1485, 2227
BsmFI	GGGAC	2 cut(s)	491, 1388
BsmI	GAATGC	2 cut(s)	382, 914
BsnI	GGCC	2 cut(s)	777, 1411
BsoBI	CYCGRG	1 cut(s)	194
Bsp119I	TTCGAA	1 cut(s)	1353
Bsp1286I	GDGCHC	5 cut(s)	399, 695, 1231, 1332, 2177
Bsp1720I	GCTNAGC	1 cut(s)	1245
Bsp19I	CCATGG	1 cut(s)	1069
Bsp68I	TCGCGA	1 cut(s)	1313
BspANI	GGCC	2 cut(s)	777, 1411
BspCNI	CTCAG	7 cut(s)	198, 645, 1157, 1237, 1482, 1495, 1982
BspFNI	CGCG	3 cut(s)	1313, 1512, 2130
BspHI	TCATGA	1 cut(s)	1725
BspLI	GGNNCC	5 cut(s)	507, 598, 1613, 1911, 2109
BspMAI	CTGCAG	1 cut(s)	873
BspMI	ACCTGC	1 cut(s)	973
BspPI	GGATC	7 cut(s)	139, 591, 604, 715, 757, 948, 1820
BspT104I	TTCGAA	1 cut(s)	1353
BspTI	CTTAAG	2 cut(s)	575, 1100
BsrBI	CCGCTC	1 cut(s)	2193
BsrDI	GCAATG	2 cut(s)	253, 1815
BsrFI	RCCGGY	1 cut(s)	1689
BsrI	ACTGG	5 cut(s)	88, 148, 278, 766, 1342
BssAI	RCCGGY	1 cut(s)	1689
BssECI	CCNNGG	5 cut(s)	1069, 1230, 1717, 1905, 2021
BssNAI	GTATAC	1 cut(s)	1303
BssSI	CACGAG	1 cut(s)	436
BssT1I	CCWWGG	2 cut(s)	1069, 2021
Bst1107I	GTATAC	1 cut(s)	1303
Bst2BI	CACGAG	1 cut(s)	436
Bst2UI	CCWGG	2 cut(s)	1260, 1719
Bst4CI	ACNGT	2 cut(s)	968, 1472
Bst6I	CTCTTC	5 cut(s)	162, 416, 846, 1421, 1913
BstAFI	CTTAAG	2 cut(s)	575, 1100
BstBI	TTCGAA	1 cut(s)	1353
BstC8I	GCNNGC	4 cut(s)	1166, 1466, 2191, 2201
BstDEI	CTNAG	9 cut(s)	206, 653, 1144, 1245, 1482, 1490, 1841, 1889, 1969
BstDSI	CCRYGG	1 cut(s)	1069
BstF5I	GGATG	5 cut(s)	268, 327, 1499, 1763, 2045
BstFNI	CGCG	3 cut(s)	1313, 1512, 2130
BstH2I	RGCGCY	1 cut(s)	1489
BstHHI	GCGC	5 cut(s)	948, 974, 1170, 1488, 1521
BstMAI	GTCTC	5 cut(s)	627, 787, 1147, 1485, 2227
BstMCI	CGRYCG	1 cut(s)	135
BstMWI	GCNNNNNNNGC	7 cut(s)	247, 1122, 1518, 1570, 1640, 1964, 2032
BstNI	CCWGG	2 cut(s)	1260, 1719
BstSCI	CCNGG	6 cut(s)	881, 999, 1230, 1258, 1634, 1717
BstSFI	CTRYAG	1 cut(s)	869
BstSLI	GKGCMC	1 cut(s)	1231
BstUI	CGCG	3 cut(s)	1313, 1512, 2130
BstV2I	GAAGAC	3 cut(s)	182, 1715, 2144
BstX2I	RGATCY	6 cut(s)	144, 596, 762, 940, 1062, 1825
BstYI	RGATCY	6 cut(s)	144, 596, 762, 940, 1062, 1825
BstZ17I	GTATAC	1 cut(s)	1303
Bsu36I	CCTNAGG	1 cut(s)	1969
BsuRI	GGCC	2 cut(s)	777, 1411
BtgI	CCRYGG	1 cut(s)	1069
BtgZI	GCGATG	2 cut(s)	1328, 1967
BtrI	CACGTC	1 cut(s)	2221
BtsCI	GGATG	5 cut(s)	268, 327, 1499, 1763, 2045
BtsI	GCAGTG	1 cut(s)	1929
BtsIMutI	CAGTG	4 cut(s)	95, 773, 1477, 1929
BtuMI	TCGCGA	1 cut(s)	1313
BveI	ACCTGC	1 cut(s)	973
Cac8I	GCNNGC	4 cut(s)	1166, 1466, 2191, 2201
CaiI	CAGNNNCTG	2 cut(s)	205, 1489
CciI	TCATGA	1 cut(s)	1725
CfoI	GCGC	5 cut(s)	948, 974, 1170, 1488, 1521
Cfr10I	RCCGGY	1 cut(s)	1689
Cfr13I	GGNCC	4 cut(s)	505, 775, 1854, 1875
CseI	GACGC	1 cut(s)	304
Csp6I	GTAC	4 cut(s)	754, 874, 1639, 1835
CviQI	GTAC	4 cut(s)	754, 874, 1639, 1835
DdeI	CTNAG	9 cut(s)	206, 653, 1144, 1245, 1482, 1490, 1841, 1889, 1969
DriI	GACNNNNNGTC	1 cut(s)	1810
Eam1104I	CTCTTC	5 cut(s)	162, 416, 846, 1421, 1913
Eam1105I	GACNNNNNGTC	1 cut(s)	1810
EarI	CTCTTC	5 cut(s)	162, 416, 846, 1421, 1913
EciI	GGCGGA	2 cut(s)	2049, 2229
Ecl136II	GAGCTC	2 cut(s)	693, 1330
Eco130I	CCWWGG	2 cut(s)	1069, 2021
Eco147I	AGGCCT	1 cut(s)	1411
Eco24I	GRGCYC	3 cut(s)	399, 695, 1332
Eco32I	GATATC	1 cut(s)	1015
Eco47I	GGWCC	3 cut(s)	505, 1854, 1875
Eco53kI	GAGCTC	2 cut(s)	693, 1330
Eco57I	CTGAAG	2 cut(s)	1007, 1637
Eco81I	CCTNAGG	1 cut(s)	1969
Eco88I	CYCGRG	1 cut(s)	194
EcoICRI	GAGCTC	2 cut(s)	693, 1330
EcoO109I	RGGNCCY	1 cut(s)	1875
EcoRI	GAATTC	1 cut(s)	823
EcoRII	CCWGG	2 cut(s)	1258, 1717
EcoRV	GATATC	1 cut(s)	1015
EcoT14I	CCWWGG	2 cut(s)	1069, 2021
EcoT38I	GRGCYC	3 cut(s)	399, 695, 1332
ErhI	CCWWGG	2 cut(s)	1069, 2021
Esp3I	CGTCTC	2 cut(s)	1485, 2227
FalI	AAGNNNNNCTT	4 cut(s)	225, 257, 896, 928
FaqI	GGGAC	2 cut(s)	491, 1388
FauI	CCCGC	1 cut(s)	385
FbaI	TGATCA	1 cut(s)	75
FblI	GTMKAC	2 cut(s)	1302, 2125
FokI	GGATG	5 cut(s)	275, 314, 1486, 1750, 2032
FriOI	GRGCYC	3 cut(s)	399, 695, 1332
FspBI	CTAG	4 cut(s)	1056, 1241, 1713, 1959
FspI	TGCGCA	1 cut(s)	1169
GlaI	GCGC	5 cut(s)	947, 973, 1169, 1487, 1520
GsuI	CTGGAG	1 cut(s)	402
HaeII	RGCGCY	1 cut(s)	1489
HaeIII	GGCC	2 cut(s)	777, 1411
HapII	CCGG	6 cut(s)	13, 882, 1001, 1232, 1635, 1690
HgaI	GACGC	1 cut(s)	304
HhaI	GCGC	5 cut(s)	948, 974, 1170, 1488, 1521
Hin6I	GCGC	5 cut(s)	946, 972, 1168, 1486, 1519
HinP1I	GCGC	5 cut(s)	946, 972, 1168, 1486, 1519
HincII	GTYRAC	1 cut(s)	1804
HindII	GTYRAC	1 cut(s)	1804
HindIII	AAGCTT	1 cut(s)	2099
HpaII	CCGG	6 cut(s)	13, 882, 1001, 1232, 1635, 1690
HphI	GGTGA	3 cut(s)	592, 1422, 2070
Hpy166II	GTNNAC	4 cut(s)	114, 1303, 1804, 2126
Hpy8I	GTNNAC	4 cut(s)	114, 1303, 1804, 2126
Hpy99I	CGWCG	4 cut(s)	320, 1556, 1559, 2035
HpyAV	CCTTC	8 cut(s)	37, 227, 898, 952, 972, 1115, 1437, 1696
HpyCH4III	ACNGT	2 cut(s)	968, 1472
HpyCH4IV	ACGT	5 cut(s)	116, 1299, 1800, 1942, 2220
HpyF10VI	GCNNNNNNNGC	7 cut(s)	247, 1122, 1518, 1570, 1640, 1964, 2032
HpyF3I	CTNAG	9 cut(s)	206, 653, 1144, 1245, 1482, 1490, 1841, 1889, 1969
HpySE526I	ACGT	5 cut(s)	116, 1299, 1800, 1942, 2220
HspAI	GCGC	5 cut(s)	946, 972, 1168, 1486, 1519
Ksp22I	TGATCA	1 cut(s)	75
LmnI	GCTCC	4 cut(s)	211, 238, 1110, 1915
LweI	GCATC	4 cut(s)	402, 1305, 1451, 1731
MaeI	CTAG	4 cut(s)	1056, 1241, 1713, 1959
MaeII	ACGT	5 cut(s)	116, 1299, 1800, 1942, 2220
MaeIII	GTNAC	3 cut(s)	1385, 1472, 1973
MbiI	CCGCTC	1 cut(s)	2193
MflI	RGATCY	6 cut(s)	144, 596, 762, 940, 1062, 1825
MhlI	GDGCHC	5 cut(s)	399, 695, 1231, 1332, 2177
MluCI	AATT	5 cut(s)	8, 320, 823, 1447, 1771
MlyI	GAGTC	4 cut(s)	1613, 1747, 2004, 2173
MmeI	TCCRAC	2 cut(s)	510, 642
MroXI	GAANNNNTTC	1 cut(s)	912
MseI	TTAA	4 cut(s)	576, 671, 1101, 1451
MslI	CAYNNNNRTG	2 cut(s)	263, 861
MspA1I	CMGCKG	2 cut(s)	713, 1631
MspCI	CTTAAG	2 cut(s)	575, 1100
MspI	CCGG	6 cut(s)	13, 882, 1001, 1232, 1635, 1690
MspR9I	CCNGG	6 cut(s)	883, 1001, 1232, 1260, 1636, 1719
Mva1269I	GAATGC	2 cut(s)	382, 914
MvaI	CCWGG	2 cut(s)	1260, 1719
MvnI	CGCG	3 cut(s)	1313, 1512, 2130
MwoI	GCNNNNNNNGC	7 cut(s)	247, 1122, 1518, 1570, 1640, 1964, 2032
NciI	CCSGG	4 cut(s)	883, 1001, 1232, 1636
NcoI	CCATGG	1 cut(s)	1069
NlaIV	GGNNCC	5 cut(s)	507, 598, 1613, 1911, 2109
NmeAIII	GCCGAG	1 cut(s)	2187
NmuCI	GTSAC	1 cut(s)	1472
NruI	TCGCGA	1 cut(s)	1313
NsbI	TGCGCA	1 cut(s)	1169
NspV	TTCGAA	1 cut(s)	1353
PaeR7I	CTCGAG	1 cut(s)	194
PagI	TCATGA	1 cut(s)	1725
PceI	AGGCCT	1 cut(s)	1411
PctI	GAATGC	2 cut(s)	382, 914
PdmI	GAANNNNTTC	1 cut(s)	912
PfeI	GAWTC	6 cut(s)	70, 1358, 1731, 1745, 1784, 2228
PflFI	GACNNNGTC	1 cut(s)	314
PflMI	CCANNNNNTGG	1 cut(s)	772
Ple19I	CGATCG	1 cut(s)	135
PleI	GAGTC	4 cut(s)	1612, 1746, 2004, 2172
PpsI	GAGTC	4 cut(s)	1612, 1746, 2004, 2172
PpuMI	RGGWCCY	1 cut(s)	1875
Psp124BI	GAGCTC	2 cut(s)	695, 1332
Psp1406I	AACGTT	1 cut(s)	1800
Psp5II	RGGWCCY	1 cut(s)	1875
Psp6I	CCWGG	2 cut(s)	1258, 1717
PspGI	CCWGG	2 cut(s)	1258, 1717
PspN4I	GGNNCC	5 cut(s)	507, 598, 1613, 1911, 2109
PspPI	GGNCC	4 cut(s)	505, 775, 1854, 1875
PspPPI	RGGWCCY	1 cut(s)	1875
PspXI	VCTCGAGB	1 cut(s)	194
PstI	CTGCAG	1 cut(s)	873
PstNI	CAGNNNCTG	2 cut(s)	205, 1489
PsuI	RGATCY	6 cut(s)	144, 596, 762, 940, 1062, 1825
PsyI	GACNNNGTC	1 cut(s)	314
PvuI	CGATCG	1 cut(s)	135
PvuII	CAGCTG	1 cut(s)	1631
RruI	TCGCGA	1 cut(s)	1313
RsaI	GTAC	4 cut(s)	755, 875, 1640, 1836
RsaNI	GTAC	4 cut(s)	754, 874, 1639, 1835
RseI	CAYNNNNRTG	2 cut(s)	263, 861
SacI	GAGCTC	2 cut(s)	695, 1332
SaqAI	TTAA	4 cut(s)	576, 671, 1101, 1451
Sau96I	GGNCC	4 cut(s)	505, 775, 1854, 1875
SchI	GAGTC	4 cut(s)	1613, 1747, 2004, 2173
ScrFI	CCNGG	6 cut(s)	883, 1001, 1232, 1260, 1636, 1719
SduI	GDGCHC	5 cut(s)	399, 695, 1231, 1332, 2177
SfaNI	GCATC	4 cut(s)	402, 1305, 1451, 1731
SfcI	CTRYAG	1 cut(s)	869
Sfr274I	CTCGAG	1 cut(s)	194
SfuI	TTCGAA	1 cut(s)	1353
SinI	GGWCC	3 cut(s)	505, 1854, 1875
SlaI	CTCGAG	1 cut(s)	194
SmiMI	CAYNNNNRTG	2 cut(s)	263, 861
SmlI	CTYRAG	4 cut(s)	194, 575, 644, 1100
SmoI	CTYRAG	4 cut(s)	194, 575, 644, 1100
Sse9I	AATT	5 cut(s)	8, 320, 823, 1447, 1771
SseBI	AGGCCT	1 cut(s)	1411
SspMI	CTAG	4 cut(s)	1056, 1241, 1713, 1959
SstI	GAGCTC	2 cut(s)	695, 1332
StuI	AGGCCT	1 cut(s)	1411
StyD4I	CCNGG	6 cut(s)	881, 999, 1230, 1258, 1634, 1717
StyI	CCWWGG	2 cut(s)	1069, 2021
TaaI	ACNGT	2 cut(s)	968, 1472
TaiI	ACGT	5 cut(s)	119, 1302, 1803, 1945, 2223
TaqI	TCGA	6 cut(s)	165, 195, 318, 399, 1183, 1353
TasI	AATT	5 cut(s)	8, 320, 823, 1447, 1771
TatI	WGTACW	1 cut(s)	753
TauI	GCSGC	3 cut(s)	253, 562, 733
TfiI	GAWTC	6 cut(s)	70, 1358, 1731, 1745, 1784, 2228
Tru1I	TTAA	4 cut(s)	576, 671, 1101, 1451
Tru9I	TTAA	4 cut(s)	576, 671, 1101, 1451
TscAI	CASTG	4 cut(s)	95, 773, 1477, 1936
TseFI	GTSAC	1 cut(s)	1472
Tsp45I	GTSAC	1 cut(s)	1472
TspDTI	ATGAA	6 cut(s)	56, 947, 971, 1121, 1931, 2150
TspGWI	ACGGA	5 cut(s)	1301, 1601, 1846, 1996, 2019
TspRI	CASTG	4 cut(s)	95, 773, 1477, 1936
Tth111I	GACNNNGTC	1 cut(s)	314
Van91I	CCANNNNNTGG	1 cut(s)	772
Vha464I	CTTAAG	2 cut(s)	575, 1100
VpaK11BI	GGWCC	3 cut(s)	505, 1854, 1875
XapI	RAATTY	3 cut(s)	320, 823, 1771
XcmI	CCANNNNNNNNNTGG	1 cut(s)	485
XhoI	CTCGAG	1 cut(s)	194
XmiI	GTMKAC	2 cut(s)	1302, 2125
XmnI	GAANNNNTTC	1 cut(s)	912
XspI	CTAG	4 cut(s)	1056, 1241, 1713, 1959

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.