Rorug07G0023900

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0008135
Physical Location & Seq
Forward (+)
1716096 .. 1721308
5213 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0023900.1

Sequence Viewer

Length: 1593 bp
ATGGCTGGAGGAGGTGCTGCAAAGGTGGATGAGCCACAGCCTCACCCACCCAAAGAACAACTACCTGGCATTTCTTACTGCATTACAAGTCCACCTCCATGGCCCGAGGCCATTCTCCTTGGGTTTCAACATTATCTTGTGATGCTTGGCACTTCAGTTCTCATTCCTAGTGCTCTTGTTCCCCAGATGGGAGGTGGAAATGAGGAGAAGGCCAAGGTCATCCAGACCCTACTCTTTGTTGCTGGTTTGAACACCTTGAAGCAAACATTGTTTGGGACTAGACTACCTGCAGTGATTGGAGGGTCATACACGTTTGTTCCCACCACAGTTTCAATCATATTAGCTAGTCGATTCAGTGATTCTCAAGACCCTATAGACAAATTTAAGAGAACAATGCGGGCAATTCAAGGTGCTCTTATTGTGGCCTCAACCCTTCAGATAGTCTTAGGCTTCAGTGGCCTTTGGCGTAATGTTGCAAGGTTTTTAAGTCCACTGTCAATTGTTCCATTGATAGGACTAGTTGGTTTTGGACTCTACGAGTTAGGTTTTCCTCTGCTTGCCAAATGTGTTGAGATCGGACTGCCAGAGCTTATCATTTTAGTATTTGTCTCTCAGTATTTGCCCCACTTATTTCACAGAGGAAAACATGTATTTGATCGTTTTGCTGTTCTATTTGCCATTGTGATCGTGTGGATTTATGCTCATCTGCTCACTGTTGGCGGGGCATATAATGATTCAGGCCCCAAAACACAAGCAAGTTGCCGTACTGACCGTGCTGGACTTATAGATGGTGCTCCATGGATAAGAGTTCCGTATCCCTTCCAATGGGGAGCACCTACATTTGATGCTGGTGAAGCCTTTGCTATGATGACTGCGTCTTTTGTTGCTCTCGTAGAGTCCACTGGTGCTTTCATTGCTGTATCAAGGTATGCTAGTGCAACTCCAGTGCCACCTGCTATTCTGAGTCGTGGGGTTGGTTGGCAGGGTGTTGGGATTCTGATTTCCGGGCTGTTTGGAACTGTGACCGGATCCTCAGTATCTGTAGAGAATGCTGGTCTTTTGGCGTTAACACGTGTTGGCAGTCGAAGGGTTGTGCAAATATCTGCTGGGTTCATGATATTTTTCTCCATTCTGGGCAAATTTGGAGCAGTCTTCGCTTCAATCCCAGGGCCCATCATTGCTGCTTTATATTGTCTGTTCTTTGCTTATGTTGGTTCGGGGGGTCTTAGCTTTCTCCAGTTCTGCAACCTGAACAGCTTTCGAACCAAGTTCATATTAGGCTTCTCTATCTTCATGGGTTTGTCTATACCACAGTACTTCAATGAGTACACCCTCCTCAACGGTTATGGTCCAGTCCACACAGGAGGAAGATGGTTTAATGATATGATCAATGTCCCTTTCCAATCGGAAGCGTTTGTGGCGGGCATTATTGCATATTTCTTGGACAACACGCTGCATCGAAATGACAACACAGTTAGGAAGGACAGGGGTAAACATTGGTGGGACAAGTTCCGGTCATTCAAGGGTGATTCAAGGAGTGAGGAGTTCTATTCATTGCCGTTCAATCTAAACAAGTATTTTCCATCTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1221 Pkinase_Tyr

Protein Analysis

530

Amino Acids

57.72

Weight (kDa)

9.3

Isoelectric Point (pI)

37.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Xan_ur_permease PF00860 37 - 440 3.2e-66 Permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 961
Acc36I ACCTGC 2 cut(s) 295, 961
AciI CCGC 3 cut(s) 397, 720, 1421
AclWI GGATC 2 cut(s) 1023, 1036
AcsI RAATTY 2 cut(s) 380, 1139
AcuI CTGAAG 3 cut(s) 138, 419, 436
AcvI CACGTG 1 cut(s) 1073
AfaI GTAC 3 cut(s) 766, 1316, 1328
AfiI CCNNNNNNNGG 3 cut(s) 188, 512, 825
AflIII ACRYGT 4 cut(s) 309, 646, 1070, 1072
AhlI ACTAGT 1 cut(s) 517
AjnI CCWGG 2 cut(s) 64, 1165
AluBI AGCT 4 cut(s) 344, 589, 1230, 1257
AluI AGCT 4 cut(s) 344, 589, 1230, 1257
Alw21I GWGCWC 4 cut(s) 175, 415, 796, 835
Alw26I GTCTC 1 cut(s) 613
AlwI GGATC 2 cut(s) 1023, 1036
AlwNI CAGNNNCTG 1 cut(s) 1040
Ama87I CYCGRG 1 cut(s) 104
AoxI GGCC 7 cut(s) 101, 108, 210, 423, 457, 739, 1169
ApaI GGGCCC 1 cut(s) 1173
ApeKI GCWGC 3 cut(s) 17, 1181, 1453
ApoI RAATTY 2 cut(s) 380, 1139
AspS9I GGNCC 5 cut(s) 102, 740, 1169, 1170, 1349
AsuC2I CCSGG 1 cut(s) 1006
AsuHPI GGTGA 3 cut(s) 35, 863, 1538
AsuII TTCGAA 1 cut(s) 1261
AvaI CYCGRG 1 cut(s) 104
AvaII GGWCC 1 cut(s) 1349
BaeGI GKGCMC 1 cut(s) 1173
BamHI GGATCC 1 cut(s) 1028
BanII GRGCYC 1 cut(s) 1173
BbrPI CACGTG 1 cut(s) 1073
BbsI GAAGAC 1 cut(s) 1144
Bbv12I GWGCWC 4 cut(s) 175, 415, 796, 835
BbvI GCAGC 3 cut(s) 4, 1168, 1440
BccI CCATC 5 cut(s) 181, 782, 1181, 1365, 1591
BceAI ACGGC 2 cut(s) 747, 1543
BciT130I CCWGG 2 cut(s) 66, 1167
BciVI GTATCC 1 cut(s) 825
BclI TGATCA 1 cut(s) 1386
BcnI CCSGG 1 cut(s) 1006
BcoDI GTCTC 1 cut(s) 613
BcuI ACTAGT 1 cut(s) 517
BfaI CTAG 5 cut(s) 168, 279, 345, 518, 933
BfmI CTRYAG 3 cut(s) 288, 372, 1041
BfuAI ACCTGC 2 cut(s) 295, 961
BfuI GTATCC 1 cut(s) 825
BisI GCNGC 3 cut(s) 18, 1182, 1454
BlsI GCNGC 3 cut(s) 19, 1183, 1455
BmcAI AGTACT 1 cut(s) 1316
Bme1390I CCNGG 3 cut(s) 66, 1006, 1167
Bme18I GGWCC 1 cut(s) 1349
BmeT110I CYCGRG 1 cut(s) 104
BmgT120I GGNCC 5 cut(s) 102, 740, 1169, 1170, 1349
BmiI GGNNCC 3 cut(s) 742, 1030, 1171
BmrFI CCNGG 3 cut(s) 66, 1006, 1167
BmsI GCATC 3 cut(s) 132, 835, 1465
BpiI GAAGAC 1 cut(s) 1144
BpmI CTGGAG 3 cut(s) 27, 927, 1220
Bpu14I TTCGAA 1 cut(s) 1261
BpuEI CTTGAG 1 cut(s) 348
BpuMI CCSGG 1 cut(s) 1006
BsaAI YACGTR 1 cut(s) 1073
BsaJI CCNNGG 7 cut(s) 98, 105, 118, 213, 797, 1165, 1166
BsaWI WCCGGW 2 cut(s) 1025, 1512
Bsc4I CCNNNNNNNGG 3 cut(s) 188, 512, 825
Bse1I ACTGG 4 cut(s) 907, 944, 1237, 1352
Bse3DI GCAATG 3 cut(s) 912, 1176, 1553
BseBI CCWGG 2 cut(s) 66, 1167
BseDI CCNNGG 7 cut(s) 98, 105, 118, 213, 797, 1165, 1166
BseGI GGATG 2 cut(s) 34, 219
BseLI CCNNNNNNNGG 3 cut(s) 188, 512, 825
BseMI GCAATG 3 cut(s) 912, 1176, 1553
BseMII CTCAG 3 cut(s) 626, 953, 1047
BseNI ACTGG 4 cut(s) 907, 944, 1237, 1352
BseRI GAGGAG 4 cut(s) 24, 218, 1325, 1556
BseSI GKGCMC 1 cut(s) 1173
BseXI GCAGC 3 cut(s) 4, 1168, 1440
BseYI CCCAGC 1 cut(s) 1106
BshFI GGCC 7 cut(s) 103, 110, 212, 425, 459, 741, 1171
BsiHKAI GWGCWC 4 cut(s) 175, 415, 796, 835
BsiHKCI CYCGRG 1 cut(s) 104
BsiSI CCGG 3 cut(s) 1005, 1026, 1513
BslFI GGGAC 3 cut(s) 289, 1379, 1517
BslI CCNNNNNNNGG 3 cut(s) 188, 512, 825
BsmAI GTCTC 1 cut(s) 613
BsmFI GGGAC 3 cut(s) 289, 1379, 1517
BsmI GAATGC 1 cut(s) 1054
BsnI GGCC 7 cut(s) 103, 110, 212, 425, 459, 741, 1171
BsoBI CYCGRG 1 cut(s) 104
Bsp119I TTCGAA 1 cut(s) 1261
Bsp120I GGGCCC 1 cut(s) 1169
Bsp1286I GDGCHC 5 cut(s) 175, 415, 796, 835, 1173
Bsp143I GATC 5 cut(s) 573, 655, 684, 1028, 1386
Bsp19I CCATGG 2 cut(s) 98, 797
BspACI CCGC 3 cut(s) 397, 720, 1421
BspANI GGCC 7 cut(s) 103, 110, 212, 425, 459, 741, 1171
BspCNI CTCAG 3 cut(s) 625, 954, 1046
BspHI TCATGA 1 cut(s) 1113
BspLI GGNNCC 3 cut(s) 742, 1030, 1171
BspMAI CTGCAG 1 cut(s) 292
BspMI ACCTGC 2 cut(s) 295, 961
BspPI GGATC 2 cut(s) 1023, 1036
BspT104I TTCGAA 1 cut(s) 1261
BsrDI GCAATG 3 cut(s) 912, 1176, 1553
BsrI ACTGG 4 cut(s) 907, 944, 1237, 1352
BssECI CCNNGG 7 cut(s) 98, 105, 118, 213, 797, 1165, 1166
BssMI GATC 5 cut(s) 573, 655, 684, 1028, 1386
BssT1I CCWWGG 4 cut(s) 98, 118, 213, 797
Bst2UI CCWGG 2 cut(s) 66, 1167
Bst4CI ACNGT 8 cut(s) 328, 495, 715, 773, 1021, 1314, 1343, 1474
BstBAI YACGTR 1 cut(s) 1073
BstBI TTCGAA 1 cut(s) 1261
BstC8I GCNNGC 3 cut(s) 399, 558, 1423
BstDEI CTNAG 5 cut(s) 445, 612, 962, 1033, 1226
BstDSI CCRYGG 2 cut(s) 98, 797
BstF5I GGATG 2 cut(s) 34, 219
BstKTI GATC 5 cut(s) 576, 658, 687, 1031, 1389
BstMAI GTCTC 1 cut(s) 613
BstMBI GATC 5 cut(s) 573, 655, 684, 1028, 1386
BstMWI GCNNNNNNNGC 5 cut(s) 456, 854, 914, 1154, 1418
BstNI CCWGG 2 cut(s) 66, 1167
BstNSI RCATGY 1 cut(s) 650
BstSCI CCNGG 3 cut(s) 64, 1004, 1165
BstSFI CTRYAG 3 cut(s) 288, 372, 1041
BstSLI GKGCMC 1 cut(s) 1173
BstV1I GCAGC 3 cut(s) 4, 1168, 1440
BstV2I GAAGAC 1 cut(s) 1144
BstX2I RGATCY 1 cut(s) 1028
BstXI CCANNNNNNTGG 1 cut(s) 99
BstYI RGATCY 1 cut(s) 1028
BsuI GTATCC 1 cut(s) 825
BsuRI GGCC 7 cut(s) 103, 110, 212, 425, 459, 741, 1171
BtgI CCRYGG 2 cut(s) 98, 797
BtsCI GGATG 2 cut(s) 34, 219
BtsI GCAGTG 1 cut(s) 297
BtsIMutI CAGTG 7 cut(s) 297, 361, 460, 491, 711, 900, 951
BveI ACCTGC 2 cut(s) 295, 961
Cac8I GCNNGC 3 cut(s) 399, 558, 1423
CaiI CAGNNNCTG 1 cut(s) 1040
CciI TCATGA 1 cut(s) 1113
Cfr13I GGNCC 5 cut(s) 102, 740, 1169, 1170, 1349
CseI GACGC 1 cut(s) 864
Csp6I GTAC 3 cut(s) 765, 1315, 1327
CviAII CATG 5 cut(s) 99, 647, 798, 1114, 1294
CviQI GTAC 3 cut(s) 765, 1315, 1327
DdeI CTNAG 5 cut(s) 445, 612, 962, 1033, 1226
DpnI GATC 5 cut(s) 575, 657, 686, 1030, 1388
DpnII GATC 5 cut(s) 573, 655, 684, 1028, 1386
Eco130I CCWWGG 4 cut(s) 98, 118, 213, 797
Eco24I GRGCYC 1 cut(s) 1173
Eco47I GGWCC 1 cut(s) 1349
Eco57I CTGAAG 3 cut(s) 138, 419, 436
Eco72I CACGTG 1 cut(s) 1073
Eco88I CYCGRG 1 cut(s) 104
EcoO109I RGGNCCY 2 cut(s) 740, 1169
EcoRII CCWGG 2 cut(s) 64, 1165
EcoT14I CCWWGG 4 cut(s) 98, 118, 213, 797
EcoT38I GRGCYC 1 cut(s) 1173
ErhI CCWWGG 4 cut(s) 98, 118, 213, 797
FaeI CATG 5 cut(s) 102, 650, 801, 1117, 1297
FalI AAGNNNNNCTT 2 cut(s) 399, 431
FaqI GGGAC 3 cut(s) 289, 1379, 1517
FatI CATG 5 cut(s) 98, 646, 797, 1113, 1293
FauI CCCGC 3 cut(s) 390, 713, 1414
FbaI TGATCA 1 cut(s) 1386
Fnu4HI GCNGC 3 cut(s) 18, 1182, 1454
FokI GGATG 2 cut(s) 41, 206
FriOI GRGCYC 1 cut(s) 1173
Fsp4HI GCNGC 3 cut(s) 18, 1182, 1454
FspBI CTAG 5 cut(s) 168, 279, 345, 518, 933
GluI GCNGC 3 cut(s) 18, 1182, 1454
GsaI CCCAGC 1 cut(s) 1110
GsuI CTGGAG 3 cut(s) 27, 927, 1220
HaeIII GGCC 7 cut(s) 103, 110, 212, 425, 459, 741, 1171
HapII CCGG 3 cut(s) 1005, 1026, 1513
HgaI GACGC 1 cut(s) 864
Hin1II CATG 5 cut(s) 102, 650, 801, 1117, 1297
HincII GTYRAC 1 cut(s) 1068
HindII GTYRAC 1 cut(s) 1068
HinfI GANTC 8 cut(s) 351, 359, 531, 734, 896, 964, 994, 1529
HpaI GTTAAC 1 cut(s) 1068
HpaII CCGG 3 cut(s) 1005, 1026, 1513
HphI GGTGA 3 cut(s) 35, 863, 1538
Hpy166II GTNNAC 7 cut(s) 92, 491, 900, 1068, 1329, 1357, 1493
Hpy188I TCNGA 5 cut(s) 438, 578, 963, 999, 1408
Hpy188III TCNNGA 3 cut(s) 223, 365, 1114
Hpy8I GTNNAC 7 cut(s) 92, 491, 900, 1068, 1329, 1357, 1493
HpyAV CCTTC 5 cut(s) 202, 443, 829, 1080, 1474
HpyCH4III ACNGT 8 cut(s) 328, 495, 715, 773, 1021, 1314, 1343, 1474
HpyCH4IV ACGT 2 cut(s) 311, 1072
HpyCH4V TGCA 9 cut(s) 20, 81, 290, 476, 938, 1096, 1245, 1433, 1456
HpyF10VI GCNNNNNNNGC 5 cut(s) 456, 854, 914, 1154, 1418
HpyF3I CTNAG 5 cut(s) 445, 612, 962, 1033, 1226
HpySE526I ACGT 2 cut(s) 311, 1072
Hsp92II CATG 5 cut(s) 102, 650, 801, 1117, 1297
Ksp22I TGATCA 1 cut(s) 1386
KspAI GTTAAC 1 cut(s) 1068
Kzo9I GATC 5 cut(s) 573, 655, 684, 1028, 1386
LmnI GCTCC 3 cut(s) 799, 830, 1145
Lsp1109I GCAGC 3 cut(s) 4, 1168, 1440
LweI GCATC 3 cut(s) 132, 835, 1465
MaeI CTAG 5 cut(s) 168, 279, 345, 518, 933
MaeII ACGT 2 cut(s) 311, 1072
MaeIII GTNAC 1 cut(s) 1021
MalI GATC 5 cut(s) 575, 657, 686, 1030, 1388
MboI GATC 5 cut(s) 573, 655, 684, 1028, 1386
MboII GAAGA 3 cut(s) 1144, 1282, 1380
MfeI CAATTG 1 cut(s) 498
MflI RGATCY 1 cut(s) 1028
MhlI GDGCHC 5 cut(s) 175, 415, 796, 835, 1173
MluCI AATT 4 cut(s) 380, 402, 498, 1139
MlyI GAGTC 3 cut(s) 525, 905, 973
MseI TTAA 4 cut(s) 384, 485, 1067, 1377
MslI CAYNNNNRTG 3 cut(s) 97, 1461, 1588
MspI CCGG 3 cut(s) 1005, 1026, 1513
MspR9I CCNGG 3 cut(s) 66, 1006, 1167
MunI CAATTG 1 cut(s) 498
Mva1269I GAATGC 1 cut(s) 1054
MvaI CCWGG 2 cut(s) 66, 1167
MwoI GCNNNNNNNGC 5 cut(s) 456, 854, 914, 1154, 1418
NciI CCSGG 1 cut(s) 1006
NcoI CCATGG 2 cut(s) 98, 797
NdeII GATC 5 cut(s) 573, 655, 684, 1028, 1386
NlaIII CATG 5 cut(s) 102, 650, 801, 1117, 1297
NlaIV GGNNCC 3 cut(s) 742, 1030, 1171
NmuCI GTSAC 1 cut(s) 1021
NspI RCATGY 1 cut(s) 650
NspV TTCGAA 1 cut(s) 1261
PagI TCATGA 1 cut(s) 1113
PaqCI CACCTGC 1 cut(s) 961
PasI CCCWGGG 1 cut(s) 1166
PciI ACATGT 1 cut(s) 646
PctI GAATGC 1 cut(s) 1054
PfeI GAWTC 5 cut(s) 351, 359, 734, 994, 1529
PflFI GACNNNGTC 1 cut(s) 874
PkrI GCNGC 3 cut(s) 19, 1183, 1455
PleI GAGTC 3 cut(s) 525, 904, 972
PmaCI CACGTG 1 cut(s) 1073
PmlI CACGTG 1 cut(s) 1073
PpsI GAGTC 3 cut(s) 525, 904, 972
Ppu21I YACGTR 1 cut(s) 1073
PscI ACATGT 1 cut(s) 646
Psp6I CCWGG 2 cut(s) 64, 1165
PspCI CACGTG 1 cut(s) 1073
PspFI CCCAGC 1 cut(s) 1106
PspGI CCWGG 2 cut(s) 64, 1165
PspN4I GGNNCC 3 cut(s) 742, 1030, 1171
PspOMI GGGCCC 1 cut(s) 1169
PspPI GGNCC 5 cut(s) 102, 740, 1169, 1170, 1349
PstI CTGCAG 1 cut(s) 292
PstNI CAGNNNCTG 1 cut(s) 1040
PsuI RGATCY 1 cut(s) 1028
PsyI GACNNNGTC 1 cut(s) 874
RsaI GTAC 3 cut(s) 766, 1316, 1328
RsaNI GTAC 3 cut(s) 765, 1315, 1327
RseI CAYNNNNRTG 3 cut(s) 97, 1461, 1588
SaqAI TTAA 4 cut(s) 384, 485, 1067, 1377
SatI GCNGC 3 cut(s) 18, 1182, 1454
Sau3AI GATC 5 cut(s) 573, 655, 684, 1028, 1386
Sau96I GGNCC 5 cut(s) 102, 740, 1169, 1170, 1349
ScaI AGTACT 1 cut(s) 1316
SchI GAGTC 3 cut(s) 525, 905, 973
ScrFI CCNGG 3 cut(s) 66, 1006, 1167
SduI GDGCHC 5 cut(s) 175, 415, 796, 835, 1173
SfaNI GCATC 3 cut(s) 132, 835, 1465
SfcI CTRYAG 3 cut(s) 288, 372, 1041
SfuI TTCGAA 1 cut(s) 1261
SinI GGWCC 1 cut(s) 1349
SmiMI CAYNNNNRTG 3 cut(s) 97, 1461, 1588
SmlI CTYRAG 1 cut(s) 363
SmoI CTYRAG 1 cut(s) 363
SpeI ACTAGT 1 cut(s) 517
Sse9I AATT 4 cut(s) 380, 402, 498, 1139
SsiI CCGC 3 cut(s) 397, 720, 1421
SspMI CTAG 5 cut(s) 168, 279, 345, 518, 933
StyD4I CCNGG 3 cut(s) 64, 1004, 1165
StyI CCWWGG 4 cut(s) 98, 118, 213, 797
TaaI ACNGT 8 cut(s) 328, 495, 715, 773, 1021, 1314, 1343, 1474
TaiI ACGT 2 cut(s) 314, 1075
TaqI TCGA 4 cut(s) 349, 1084, 1261, 1459
TasI AATT 4 cut(s) 380, 402, 498, 1139
TatI WGTACW 2 cut(s) 1314, 1326
TfiI GAWTC 5 cut(s) 351, 359, 734, 994, 1529
Tru1I TTAA 4 cut(s) 384, 485, 1067, 1377
Tru9I TTAA 4 cut(s) 384, 485, 1067, 1377
TscAI CASTG 7 cut(s) 297, 361, 460, 498, 718, 907, 951
TseFI GTSAC 1 cut(s) 1021
TseI GCWGC 3 cut(s) 17, 1181, 1453
Tsp45I GTSAC 1 cut(s) 1021
TspDTI ATGAA 5 cut(s) 901, 1102, 1261, 1282, 1542
TspGWI ACGGA 1 cut(s) 801
TspRI CASTG 7 cut(s) 297, 361, 460, 498, 718, 907, 951
Tth111I GACNNNGTC 1 cut(s) 874
VpaK11BI GGWCC 1 cut(s) 1349
XapI RAATTY 2 cut(s) 380, 1139
XceI RCATGY 1 cut(s) 650
XcmI CCANNNNNNNNNTGG 1 cut(s) 191
XspI CTAG 5 cut(s) 168, 279, 345, 518, 933
ZrmI AGTACT 1 cut(s) 1316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.