Rw7G012920

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0008135
Physical Location & Seq
Forward (+)
12288377 .. 12291112
2736 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G012920.1

Sequence Viewer

Length: 1212 bp
ATGGGGGTTAGGGCACGGCCCAATTTGAGCGGCGAAGCCGAAGACGACGCCGGGACCGACACCATTCCGCTCATGATTTCGAAGCTCAAGACGGCGTTTCTCCCCGCCGACTTCCAACGCGCCGAGCGGGCCTTACTCGCCAGAGAATCCAATCTGAGGCGAGAAATCGAGAAGCTGAAGAAGGAAAACGCGAGGCTTCTGGAAGACAACCGATTCCGGGAGCTGGCGATGCTGAGAGTGGAGACTGATTCGAAAGGTGTGATCTTTGAGCAGAGGAGGAAGATCAGGGAGCTGGAGACGCAGAAATTGAAGCTCGAGGCGTTGGTTAAGAGCGAGTATGAAGGATGGAAGGGGCTGATTTCGAAGCTGGAGGGAGATGCGAAGAAATTGAGCGGCCAAAAGGTTGTGGGTTTCAGGAGCAATGTTCGTGTGGTGGTGAAATTAGAGAGGGAGTTTGGTGCTAATGAAGTTGGAGGCGTGGCGTCCAGTGCAGGGAGCTCGATTCTCAGTGAAGGAAATGGAGGTGTCAAAGTTTCAGGTATTCGGAAATCTTCATCTGAAGATGCTGGTTTGATTGATAGACATAATGAGTGTGCTCCCAGGGAAATCGAGATGCTGTGTGAGAATTCTGAAATACATAAAGGCACTGGATCTTTGGGTTTGACTGGTGTAGAAAGGAAGCTAAGGGTTCTTAAAAGAAAACCAGCGTCATCTTTAAGTGGTAGTGATGATGAAAACGGGGGTGGCAATTTTAAGAAAGATAAAAACTCAGACAGTGAGATGAAGCAACTCCAGAAGTTGAGCATTCAGCCTGATGGTCCTCTTATCAAGCAATGCACAGAAATACTTGATTTTTCTGGTAGTAATGACTCTGAAAATCAAAATTTACCAAGTCATGAGCAAGTGAACTCAAGGCAATCTGAAGAGAAAATTATGGCAGAACAGACGTCACAACCTTTTCTTAAGGCTCCTACGGAGGTGAGTAGTGCTTTTGATAATGATCATCATGCCGATCTTAGTTCTAGTTGCAGTTCAAGTTCCTCTGACTCTGAAGATGACTGTCTACTACTATCTGCACTTGATGATATTATGCCAAAAGCCAAAGCAAGAGAGGTAAAGATAAATGACATTTGTACTGCTTTTGCAATAGTGCCGTTTCTGCTTTCTACTTGTAATATTTCCCTTTTCTCTTTTAGTATGATTAATTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

403

Amino Acids

44.26

Weight (kDa)

5.68

Isoelectric Point (pI)

46.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 950
AccBSI CCGCTC 4 cut(s) 30, 70, 127, 393
AccI GTMKAC 1 cut(s) 1063
AccII CGCG 2 cut(s) 120, 191
AciI CCGC 5 cut(s) 30, 68, 105, 127, 393
AclWI GGATC 1 cut(s) 658
AcoI YGGCCR 1 cut(s) 394
AcsI RAATTY 2 cut(s) 625, 883
AcuI CTGAAG 4 cut(s) 197, 579, 942, 1071
AcyI GRCGYC 3 cut(s) 48, 482, 947
AfaI GTAC 1 cut(s) 1135
AfiI CCNNNNNNNGG 4 cut(s) 156, 217, 223, 492
AflII CTTAAG 1 cut(s) 962
AgsI TTSAA 2 cut(s) 310, 1035
AjnI CCWGG 1 cut(s) 599
AluBI AGCT 8 cut(s) 85, 175, 223, 292, 313, 367, 498, 682
AluI AGCT 8 cut(s) 85, 175, 223, 292, 313, 367, 498, 682
Alw21I GWGCWC 2 cut(s) 500, 598
Alw26I GTCTC 2 cut(s) 236, 290
AlwI GGATC 1 cut(s) 658
Ama87I CYCGRG 1 cut(s) 314
AoxI GGCC 3 cut(s) 17, 129, 394
ApoI RAATTY 2 cut(s) 625, 883
AseI ATTAAT 1 cut(s) 1203
AspLEI GCGC 1 cut(s) 122
AspS9I GGNCC 4 cut(s) 18, 54, 129, 818
AsuC2I CCSGG 2 cut(s) 52, 218
AsuHPI GGTGA 2 cut(s) 448, 991
AsuII TTCGAA 3 cut(s) 80, 251, 362
AvaI CYCGRG 1 cut(s) 314
AvaII GGWCC 2 cut(s) 54, 818
BaeGI GKGCMC 1 cut(s) 16
BanII GRGCYC 1 cut(s) 500
BbsI GAAGAC 2 cut(s) 48, 210
Bbv12I GWGCWC 2 cut(s) 500, 598
BccI CCATC 2 cut(s) 339, 809
BceAI ACGGC 3 cut(s) 32, 108, 1138
BcgI CGANNNNNNTGC 2 cut(s) 480, 514
BciT130I CCWGG 1 cut(s) 601
BclI TGATCA 1 cut(s) 1000
BcnI CCSGG 2 cut(s) 52, 218
BcoDI GTCTC 2 cut(s) 236, 290
BfaI CTAG 1 cut(s) 1023
BfrI CTTAAG 1 cut(s) 962
BglI GCCNNNNNGGC 1 cut(s) 128
BisI GCNGC 2 cut(s) 31, 394
BlsI GCNGC 2 cut(s) 32, 395
Bme1390I CCNGG 3 cut(s) 52, 218, 601
Bme18I GGWCC 2 cut(s) 54, 818
BmeT110I CYCGRG 1 cut(s) 314
BmgT120I GGNCC 4 cut(s) 18, 54, 129, 818
BmiI GGNNCC 2 cut(s) 55, 969
BmrFI CCNGG 3 cut(s) 52, 218, 601
BmsI GCATC 4 cut(s) 219, 367, 553, 603
BpiI GAAGAC 2 cut(s) 48, 210
BpmI CTGGAG 3 cut(s) 314, 389, 776
Bpu10I CCTNAGC 1 cut(s) 683
Bpu14I TTCGAA 3 cut(s) 80, 251, 362
BpuEI CTTGAG 2 cut(s) 71, 895
BpuMI CCSGG 2 cut(s) 52, 218
BsaBI GATNNNNATC 1 cut(s) 999
BsaHI GRCGYC 3 cut(s) 48, 482, 947
BsaJI CCNNGG 2 cut(s) 599, 600
Bsc4I CCNNNNNNNGG 4 cut(s) 156, 217, 223, 492
Bse1I ACTGG 3 cut(s) 486, 652, 670
Bse3DI GCAATG 2 cut(s) 427, 839
Bse8I GATNNNNATC 1 cut(s) 999
BseBI CCWGG 1 cut(s) 601
BseDI CCNNGG 2 cut(s) 599, 600
BseGI GGATG 1 cut(s) 350
BseJI GATNNNNATC 1 cut(s) 999
BseLI CCNNNNNNNGG 4 cut(s) 156, 217, 223, 492
BseMI GCAATG 2 cut(s) 427, 839
BseMII CTCAG 4 cut(s) 146, 224, 520, 783
BseNI ACTGG 3 cut(s) 486, 652, 670
BseRI GAGGAG 1 cut(s) 289
BseSI GKGCMC 1 cut(s) 16
BsgI GTGCAG 2 cut(s) 510, 1059
Bsh1236I CGCG 2 cut(s) 120, 191
BshFI GGCC 3 cut(s) 19, 131, 396
BsiHKAI GWGCWC 2 cut(s) 500, 598
BsiHKCI CYCGRG 1 cut(s) 314
BsiSI CCGG 2 cut(s) 51, 217
BslFI GGGAC 1 cut(s) 67
BslI CCNNNNNNNGG 4 cut(s) 156, 217, 223, 492
BsmAI GTCTC 2 cut(s) 236, 290
BsmBI CGTCTC 1 cut(s) 290
BsmFI GGGAC 1 cut(s) 67
BsmI GAATGC 1 cut(s) 804
BsnI GGCC 3 cut(s) 19, 131, 396
BsoBI CYCGRG 1 cut(s) 314
Bsp119I TTCGAA 3 cut(s) 80, 251, 362
Bsp1286I GDGCHC 3 cut(s) 16, 500, 598
Bsp143I GATC 5 cut(s) 261, 282, 650, 1000, 1012
BspACI CCGC 5 cut(s) 30, 68, 105, 127, 393
BspANI GGCC 3 cut(s) 19, 131, 396
BspCNI CTCAG 4 cut(s) 147, 225, 519, 782
BspFNI CGCG 2 cut(s) 120, 191
BspHI TCATGA 2 cut(s) 72, 895
BspLI GGNNCC 2 cut(s) 55, 969
BspPI GGATC 1 cut(s) 658
BspT104I TTCGAA 3 cut(s) 80, 251, 362
BspTI CTTAAG 1 cut(s) 962
BsrBI CCGCTC 4 cut(s) 30, 70, 127, 393
BsrDI GCAATG 2 cut(s) 427, 839
BsrI ACTGG 3 cut(s) 486, 652, 670
BssECI CCNNGG 2 cut(s) 599, 600
BssMI GATC 5 cut(s) 261, 282, 650, 1000, 1012
BssNI GRCGYC 3 cut(s) 48, 482, 947
Bst2UI CCWGG 1 cut(s) 601
Bst4CI ACNGT 2 cut(s) 776, 1061
Bst6I CTCTTC 1 cut(s) 918
BstACI GRCGYC 3 cut(s) 48, 482, 947
BstAFI CTTAAG 1 cut(s) 962
BstBI TTCGAA 3 cut(s) 80, 251, 362
BstC8I GCNNGC 2 cut(s) 129, 225
BstDEI CTNAG 6 cut(s) 155, 233, 506, 683, 769, 1016
BstF5I GGATG 1 cut(s) 350
BstFNI CGCG 2 cut(s) 120, 191
BstHHI GCGC 1 cut(s) 122
BstKTI GATC 5 cut(s) 264, 285, 653, 1003, 1015
BstMAI GTCTC 2 cut(s) 236, 290
BstMBI GATC 5 cut(s) 261, 282, 650, 1000, 1012
BstMWI GCNNNNNNNGC 6 cut(s) 128, 137, 229, 298, 488, 1159
BstNI CCWGG 1 cut(s) 601
BstSCI CCNGG 3 cut(s) 50, 216, 599
BstSLI GKGCMC 1 cut(s) 16
BstUI CGCG 2 cut(s) 120, 191
BstV2I GAAGAC 2 cut(s) 48, 210
BstX2I RGATCY 1 cut(s) 650
BstYI RGATCY 1 cut(s) 650
BsuRI GGCC 3 cut(s) 19, 131, 396
BtgZI GCGATG 1 cut(s) 242
BtsCI GGATG 1 cut(s) 350
BtsIMutI CAGTG 4 cut(s) 493, 514, 645, 781
Cac8I GCNNGC 2 cut(s) 129, 225
CciI TCATGA 2 cut(s) 72, 895
CfoI GCGC 1 cut(s) 122
Cfr13I GGNCC 4 cut(s) 18, 54, 129, 818
CseI GACGC 4 cut(s) 56, 307, 471, 696
Csp6I GTAC 1 cut(s) 1134
CviAII CATG 3 cut(s) 73, 896, 1007
CviQI GTAC 1 cut(s) 1134
DdeI CTNAG 6 cut(s) 155, 233, 506, 683, 769, 1016
DpnI GATC 5 cut(s) 263, 284, 652, 1002, 1014
DpnII GATC 5 cut(s) 261, 282, 650, 1000, 1012
EaeI YGGCCR 1 cut(s) 394
Eam1104I CTCTTC 1 cut(s) 918
EarI CTCTTC 1 cut(s) 918
Ecl136II GAGCTC 1 cut(s) 498
Eco24I GRGCYC 1 cut(s) 500
Eco47I GGWCC 2 cut(s) 54, 818
Eco53kI GAGCTC 1 cut(s) 498
Eco57I CTGAAG 4 cut(s) 197, 579, 942, 1071
Eco88I CYCGRG 1 cut(s) 314
EcoICRI GAGCTC 1 cut(s) 498
EcoRI GAATTC 1 cut(s) 625
EcoRII CCWGG 1 cut(s) 599
EcoT38I GRGCYC 1 cut(s) 500
Esp3I CGTCTC 1 cut(s) 290
FaeI CATG 3 cut(s) 76, 899, 1010
FaiI YATR 9 cut(s) 74, 339, 585, 639, 897, 935, 1008, 1091, 1199
FaqI GGGAC 1 cut(s) 67
FatI CATG 3 cut(s) 72, 895, 1006
FauI CCCGC 2 cut(s) 112, 120
FbaI TGATCA 1 cut(s) 1000
FblI GTMKAC 1 cut(s) 1063
Fnu4HI GCNGC 2 cut(s) 31, 394
FokI GGATG 1 cut(s) 357
FriOI GRGCYC 1 cut(s) 500
Fsp4HI GCNGC 2 cut(s) 31, 394
FspBI CTAG 1 cut(s) 1023
GlaI GCGC 1 cut(s) 121
GluI GCNGC 2 cut(s) 31, 394
GsuI CTGGAG 3 cut(s) 314, 389, 776
HaeIII GGCC 3 cut(s) 19, 131, 396
HapII CCGG 2 cut(s) 51, 217
HgaI GACGC 4 cut(s) 56, 307, 471, 696
HhaI GCGC 1 cut(s) 122
Hin1I GRCGYC 3 cut(s) 48, 482, 947
Hin1II CATG 3 cut(s) 76, 899, 1010
Hin6I GCGC 1 cut(s) 120
HinP1I GCGC 1 cut(s) 120
HinfI GANTC 6 cut(s) 146, 213, 248, 502, 869, 1046
HpaII CCGG 2 cut(s) 51, 217
HphI GGTGA 2 cut(s) 448, 991
Hpy166II GTNNAC 2 cut(s) 907, 1064
Hpy188I TCNGA 9 cut(s) 156, 546, 559, 631, 772, 874, 922, 1045, 1051
Hpy188III TCNNGA 8 cut(s) 73, 88, 169, 200, 415, 610, 793, 896
Hpy8I GTNNAC 2 cut(s) 907, 1064
Hpy99I CGWCG 1 cut(s) 50
HpyAV CCTTC 4 cut(s) 175, 335, 343, 506
HpyCH4III ACNGT 2 cut(s) 776, 1061
HpyCH4IV ACGT 1 cut(s) 947
HpyCH4V TGCA 5 cut(s) 491, 837, 1029, 1076, 1145
HpyF10VI GCNNNNNNNGC 6 cut(s) 128, 137, 229, 298, 488, 1159
HpyF3I CTNAG 6 cut(s) 155, 233, 506, 683, 769, 1016
HpySE526I ACGT 1 cut(s) 947
Hsp92I GRCGYC 3 cut(s) 48, 482, 947
Hsp92II CATG 3 cut(s) 76, 899, 1010
HspAI GCGC 1 cut(s) 120
Ksp22I TGATCA 1 cut(s) 1000
Kzo9I GATC 5 cut(s) 261, 282, 650, 1000, 1012
LmnI GCTCC 6 cut(s) 220, 289, 417, 495, 601, 973
LweI GCATC 4 cut(s) 219, 367, 553, 603
MaeI CTAG 1 cut(s) 1023
MaeII ACGT 1 cut(s) 947
MaeIII GTNAC 1 cut(s) 948
MalI GATC 5 cut(s) 263, 284, 652, 1002, 1014
MbiI CCGCTC 4 cut(s) 30, 70, 127, 393
MboI GATC 5 cut(s) 261, 282, 650, 1000, 1012
MboII GAAGA 9 cut(s) 53, 190, 215, 292, 394, 543, 572, 935, 1064
MflI RGATCY 1 cut(s) 650
MhlI GDGCHC 3 cut(s) 16, 500, 598
MluCI AATT 9 cut(s) 22, 305, 386, 440, 625, 748, 883, 930, 1204
MlyI GAGTC 2 cut(s) 863, 1040
MmeI TCCRAC 2 cut(s) 139, 451
MseI TTAA 7 cut(s) 327, 693, 716, 753, 963, 1203, 1210
MspCI CTTAAG 1 cut(s) 962
MspI CCGG 2 cut(s) 51, 217
MspR9I CCNGG 3 cut(s) 52, 218, 601
Mva1269I GAATGC 1 cut(s) 804
MvaI CCWGG 1 cut(s) 601
MvnI CGCG 2 cut(s) 120, 191
MwoI GCNNNNNNNGC 6 cut(s) 128, 137, 229, 298, 488, 1159
NciI CCSGG 2 cut(s) 52, 218
NdeII GATC 5 cut(s) 261, 282, 650, 1000, 1012
NlaIII CATG 3 cut(s) 76, 899, 1010
NlaIV GGNNCC 2 cut(s) 55, 969
NmeAIII GCCGAG 1 cut(s) 148
NmuCI GTSAC 1 cut(s) 948
NspV TTCGAA 3 cut(s) 80, 251, 362
PaeR7I CTCGAG 1 cut(s) 314
PagI TCATGA 2 cut(s) 72, 895
PasI CCCWGGG 1 cut(s) 600
PcsI WCGNNNNNNNCGW 1 cut(s) 54
PctI GAATGC 1 cut(s) 804
PfeI GAWTC 4 cut(s) 146, 213, 248, 502
PfoI TCCNGGA 1 cut(s) 216
PkrI GCNGC 2 cut(s) 32, 395
PleI GAGTC 2 cut(s) 863, 1040
PpsI GAGTC 2 cut(s) 863, 1040
PshBI ATTAAT 1 cut(s) 1203
Psp124BI GAGCTC 1 cut(s) 500
Psp6I CCWGG 1 cut(s) 599
PspGI CCWGG 1 cut(s) 599
PspN4I GGNNCC 2 cut(s) 55, 969
PspPI GGNCC 4 cut(s) 18, 54, 129, 818
PspXI VCTCGAGB 1 cut(s) 314
PsuI RGATCY 1 cut(s) 650
RsaI GTAC 1 cut(s) 1135
RsaNI GTAC 1 cut(s) 1134
SacI GAGCTC 1 cut(s) 500
SaqAI TTAA 7 cut(s) 327, 693, 716, 753, 963, 1203, 1210
SatI GCNGC 2 cut(s) 31, 394
Sau3AI GATC 5 cut(s) 261, 282, 650, 1000, 1012
Sau96I GGNCC 4 cut(s) 18, 54, 129, 818
SchI GAGTC 2 cut(s) 863, 1040
ScrFI CCNGG 3 cut(s) 52, 218, 601
SduI GDGCHC 3 cut(s) 16, 500, 598
SfaNI GCATC 4 cut(s) 219, 367, 553, 603
Sfr274I CTCGAG 1 cut(s) 314
SfuI TTCGAA 3 cut(s) 80, 251, 362
SinI GGWCC 2 cut(s) 54, 818
SlaI CTCGAG 1 cut(s) 314
SmlI CTYRAG 4 cut(s) 86, 314, 910, 962
SmoI CTYRAG 4 cut(s) 86, 314, 910, 962
Sse9I AATT 9 cut(s) 22, 305, 386, 440, 625, 748, 883, 930, 1204
SsiI CCGC 5 cut(s) 30, 68, 105, 127, 393
SspI AATATT 1 cut(s) 1177
SspMI CTAG 1 cut(s) 1023
SstI GAGCTC 1 cut(s) 500
StyD4I CCNGG 3 cut(s) 50, 216, 599
TaaI ACNGT 2 cut(s) 776, 1061
TaiI ACGT 1 cut(s) 950
TaqI TCGA 7 cut(s) 80, 168, 251, 315, 362, 500, 609
TaqII GACCGA 1 cut(s) 71
TasI AATT 9 cut(s) 22, 305, 386, 440, 625, 748, 883, 930, 1204
TatI WGTACW 1 cut(s) 1133
TauI GCSGC 2 cut(s) 33, 396
TfiI GAWTC 4 cut(s) 146, 213, 248, 502
Tru1I TTAA 7 cut(s) 327, 693, 716, 753, 963, 1203, 1210
Tru9I TTAA 7 cut(s) 327, 693, 716, 753, 963, 1203, 1210
TscAI CASTG 4 cut(s) 493, 514, 652, 781
TseFI GTSAC 1 cut(s) 948
Tsp45I GTSAC 1 cut(s) 948
TspDTI ATGAA 5 cut(s) 354, 480, 543, 747, 797
TspGWI ACGGA 1 cut(s) 989
TspRI CASTG 4 cut(s) 493, 514, 652, 781
Vha464I CTTAAG 1 cut(s) 962
VpaK11BI GGWCC 2 cut(s) 54, 818
VspI ATTAAT 1 cut(s) 1203
XapI RAATTY 2 cut(s) 625, 883
XhoI CTCGAG 1 cut(s) 314
XmiI GTMKAC 1 cut(s) 1063
XspI CTAG 1 cut(s) 1023
ZraI GACGTC 1 cut(s) 948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.