Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0008311

Physical Location & Seq

Genomic Coordinates Forward (+)

19550174 .. 19552964

Gene Structure 2791 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1121000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1863 bp

ATGGAACAATTCCGGCATATTGGGGAGGTTTTGGGAAGTATGAAGGCTCTGATGATGTTAACAGATGAGATTCTGATCAATCAATGCCAGTGCTGTTTGTTGCATGATGTGTTCACGTTGGCGTTTGACACGATCGGAGAGGAGATCCGACTGAACTTGAAACTCGAAGAGAAGAAGACAAAATGGAAAGCGCTCGAGCAGCCTCTGAGGGAGCTGCACAGAGTTTTCAAGGAAGGGGAGCTGTACATTCGGCATTGCATGGATGCAAAAAACTGGTGGGGGAAAACAATCCTCCTCCATCAGAACAAGGACTGCGTCGAATTTCACATTCATAACTTGTTCTGCTACCATGCGGCCGTGATTGAGGCGATTGAGAATGCTGGAGAGATTGCGGGGCTCGATCAGGAGGAGATGCAGAAGAAGAGGATTCTGCTCGCAAGGAAGTATGATAGAGAGTGGAATGACCCCAAACTTTTCCAATGGAGATTTGGGAAGCAGTATTTGGTCCCGAAGGCGATCTGCAACAGGTTGGAGAGTGCTTGGAGGGAAGATAGATGGCGGCTTGTCGAAACTCTGAAGGTGAAAAAGATTTCAGGATCCTTTGGTTTGACGAGGAATGAGCAGCAACTTGGAGACTTGCTGCTCAAGAAACTTGGTGGGTCGGAAACCTTAAATGGGAAGCTCTTTCCGAGCTCAATCTTACTGGAATCCGACTATTACAGCGTTAGGCGGTGGTTAGGAGGAGGAACGCAGTACAAGGAGATCCAGTGGTTGGGGCAGAACTTTGCCATGAGACACTTCTTTGGGGATATTGAGCCGTTGAATTCTGAGATTTCAACTCTCCTCGCACTTTCCCACCCCAATGTGCTGCAGTACCTTTCGGGGTTTTATGACGAGGAGAAGAAGGAATGCTTTCTTGTTATGGAGTTGATGATCAAGGATCTGCGCTGCTACATGAAGGAAAACTGCGGCGCGAGAAGGCAGGTTTTGTTTTCAATCCCGGTAGTGGTTGATATCATGCTTCAGATTGCAAGAGGCATGGAATATCTGCACTCTAGGAAGATCTACCATGGGGAGTTGAACCCCTTGAATGTCTTTCTCAAGGCAAGGAGCTGCACAGAAGGCTATTTCCAAGTAAAAGTCTCGGGTTTCAGTTTATCATCCGTGCGCAAACCTACTTACAGAAAATCACAGCAGAAGAATGAAATCAACCCTTTGATTTGGTGTGCCCCGGAAGTCCTAGCTGAGCAAGAGCAACCAGGAAACAAAGGTCGTACCAAATACACGGAGAAAGCGGATGTATACAGCTTCGCAATGCTTTGCTTTGAGATCTTGACCGGGAAGGTTCCCTTCGAAGATTCACATCTCCAAGGGGACAAGATGAGCAACACTATAAGAGCAGGATGGAGGCCTCTCTTCCCATACCCTTCACCAAAATACCTCGTCAATTTAACCAAGAGATGCTGGCACAGTGACCCGTCTCAGCGCCTGAGCTTCTCGTCCATCTGTCGCATTTTGCGCTACATAAAGAAGTTCCTTACTCTGAACCCCTATGACGATCAGCCTTTACTGCAATCCCCTCATATGGATTACTGCGAGATAGAGTCATGGTTCCTGAAGAATAGTTCAGCTGCCGAATACGTTGATTTATCCTCAATATCGCAACTTCCATTCCAAATGTTCTCTTACCGGCTAGGGGAGAAGGAAAAGACTAGCCCGGACATCGTGAGGGTTAGGAGTCTGGATTCCTTAAGTGATACAGCATCAGCGATTTGCAAGCAAGAATCTCCGAACGGCAAAGTTGACATTGTGTCCATTGCAGAAGATCCATTCGTACCACTAAGCGACTCGAGGCTCCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF1221 Pkinase_Tyr

Protein Analysis

621

Amino Acids

72.11

Weight (kDa)

8.4

Isoelectric Point (pI)

51.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF1221	PF06760	22 - 237	6.6e-104	Protein of unknown function (DUF1221)
PK_Tyr_Ser-Thr	PF07714	274 - 505	1.5e-37	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	274 - 503	1.3e-29	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008311)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G64300 AT1G64300 AT5G41730 AT5G41730
fragaria_vesca	FvH4_5g03980 FvH4_5g03980 FvH4_5g03980
malus_domestica	MD06G1101300.v1.1 MD14G1121000.v1.1
prunus_persica	Prupe.5G114700_v2.0.a1 Prupe.5G114700_v2.0.a1
pyrus_communis	pycom06g09650
rosa_chinensis	RchiOBHm_Chr7g0195511
rosa_laevigata	RLG00000004113
rosa_multiflora	Rmu_sc0007727.1_g000023 Rmu_ssc0000078.1_g000002
rosa_rugosa	Rorug07G0023900
rosa_samantha	Rh7AG151500 Rh7BG153200 Rh7CG156700 Rh7DG152500
rosa_wichuraiana	Rw2G030980 Rw7G012940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1169
Acc36I	ACCTGC	1 cut(s)	973
AccB7I	CCANNNNNTGG	1 cut(s)	772
AccI	GTMKAC	1 cut(s)	1302
AccII	CGCG	1 cut(s)	974
AciI	CCGC	6 cut(s)	353, 392, 559, 730, 969, 1295
AclWI	GGATC	6 cut(s)	139, 591, 604, 757, 948, 1820
AcoI	YGGCCR	1 cut(s)	354
AcsI	RAATTY	2 cut(s)	320, 823
AcuI	CTGAAG	3 cut(s)	596, 1007, 1637
AfaI	GTAC	5 cut(s)	245, 755, 875, 1276, 1836
AfeI	AGCGCT	1 cut(s)	192
AfiI	CCNNNNNNNGG	5 cut(s)	675, 772, 1006, 1585, 1696
AflII	CTTAAG	1 cut(s)	1750
AgsI	TTSAA	7 cut(s)	160, 229, 823, 837, 996, 1081, 1090
AhdI	GACNNNNNGTC	1 cut(s)	1810
AjnI	CCWGG	1 cut(s)	1258
AjuI	GAANNNNNNNTTGG	2 cut(s)	485, 517
AluBI	AGCT	9 cut(s)	214, 241, 682, 693, 1113, 1244, 1308, 1494, 1631
AluI	AGCT	9 cut(s)	214, 241, 682, 693, 1113, 1244, 1308, 1494, 1631
Alw21I	GWGCWC	1 cut(s)	695
Alw26I	GTCTC	4 cut(s)	627, 787, 1147, 1485
AlwI	GGATC	6 cut(s)	139, 591, 604, 757, 948, 1820
AlwNI	CAGNNNCTG	2 cut(s)	205, 1489
Ama87I	CYCGRG	3 cut(s)	194, 1144, 1849
Aor51HI	AGCGCT	1 cut(s)	192
AoxI	GGCC	2 cut(s)	354, 1409
ApeKI	GCWGC	8 cut(s)	199, 214, 622, 640, 868, 948, 1113, 1631
ApoI	RAATTY	2 cut(s)	320, 823
Asp700I	GAANNNNTTC	1 cut(s)	912
AspLEI	GCGC	6 cut(s)	193, 948, 974, 1170, 1488, 1521
AspS9I	GGNCC	1 cut(s)	505
AsuC2I	CCSGG	4 cut(s)	1001, 1232, 1339, 1718
AsuHPI	GGTGA	2 cut(s)	592, 1422
AsuII	TTCGAA	1 cut(s)	1353
AvaI	CYCGRG	3 cut(s)	194, 1144, 1849
AvaII	GGWCC	1 cut(s)	505
BaeGI	GKGCMC	1 cut(s)	1231
BamHI	GGATCC	1 cut(s)	596
BanII	GRGCYC	2 cut(s)	399, 695
BbsI	GAAGAC	1 cut(s)	182
Bbv12I	GWGCWC	1 cut(s)	695
BbvI	GCAGC	8 cut(s)	201, 211, 627, 634, 855, 935, 1100, 1618
BccI	CCATC	4 cut(s)	306, 549, 1398, 1511
BceAI	ACGGC	3 cut(s)	341, 802, 1810
BciT130I	CCWGG	1 cut(s)	1260
BclI	TGATCA	2 cut(s)	75, 933
BcnI	CCSGG	4 cut(s)	1001, 1232, 1339, 1718
BcoDI	GTCTC	4 cut(s)	627, 787, 1147, 1485
BfaI	CTAG	4 cut(s)	1056, 1241, 1694, 1713
BfmI	CTRYAG	1 cut(s)	869
BfoI	RGCGCY	2 cut(s)	194, 1489
BfrI	CTTAAG	1 cut(s)	1750
BfuAI	ACCTGC	1 cut(s)	973
BglII	AGATCT	2 cut(s)	1062, 1329
BlpI	GCTNAGC	1 cut(s)	1245
Bme1390I	CCNGG	5 cut(s)	1001, 1232, 1260, 1339, 1718
Bme18I	GGWCC	1 cut(s)	505
BmeRI	GACNNNNNGTC	1 cut(s)	1810
BmeT110I	CYCGRG	3 cut(s)	194, 1144, 1849
BmgT120I	GGNCC	1 cut(s)	505
BmiI	GGNNCC	5 cut(s)	507, 598, 1347, 1613, 1856
BmrFI	CCNGG	5 cut(s)	1001, 1232, 1260, 1339, 1718
BmsI	GCATC	4 cut(s)	253, 402, 1451, 1772
BpiI	GAAGAC	1 cut(s)	182
BpmI	CTGGAG	1 cut(s)	402
Bpu10I	CCTNAGC	1 cut(s)	1490
Bpu1102I	GCTNAGC	1 cut(s)	1245
Bpu14I	TTCGAA	1 cut(s)	1353
BpuEI	CTTGAG	2 cut(s)	629, 1085
BpuMI	CCSGG	4 cut(s)	1001, 1232, 1339, 1718
BsaBI	GATNNNNATC	2 cut(s)	74, 1362
BsaJI	CCNNGG	3 cut(s)	1069, 1230, 1369
BsaXI	ACNNNNNCTCC	2 cut(s)	752, 782
Bsc4I	CCNNNNNNNGG	5 cut(s)	675, 772, 1006, 1585, 1696
Bse118I	RCCGGY	1 cut(s)	1689
Bse1I	ACTGG	4 cut(s)	88, 278, 708, 766
Bse3DI	GCAATG	3 cut(s)	253, 1320, 1815
Bse8I	GATNNNNATC	2 cut(s)	74, 1362
BseBI	CCWGG	1 cut(s)	1260
BseDI	CCNNGG	3 cut(s)	1069, 1230, 1369
BseGI	GGATG	4 cut(s)	268, 1160, 1303, 1409
BseJI	GATNNNNATC	2 cut(s)	74, 1362
BseLI	CCNNNNNNNGG	5 cut(s)	675, 772, 1006, 1585, 1696
BseMI	GCAATG	3 cut(s)	253, 1320, 1815
BseMII	CTCAG	5 cut(s)	197, 819, 1236, 1481, 1496
BseNI	ACTGG	4 cut(s)	88, 278, 708, 766
BseRI	GAGGAG	6 cut(s)	155, 284, 422, 756, 833, 911
BseSI	GKGCMC	1 cut(s)	1231
BseX3I	CGGCCG	1 cut(s)	354
BseXI	GCAGC	8 cut(s)	201, 211, 627, 634, 855, 935, 1100, 1618
BsgI	GTGCAG	3 cut(s)	200, 1034, 1099
Bsh1236I	CGCG	1 cut(s)	974
Bsh1285I	CGRYCG	2 cut(s)	135, 357
BshFI	GGCC	2 cut(s)	356, 1411
BsiEI	CGRYCG	2 cut(s)	135, 357
BsiHKAI	GWGCWC	1 cut(s)	695
BsiHKCI	CYCGRG	3 cut(s)	194, 1144, 1849
BsiSI	CCGG	6 cut(s)	13, 1001, 1232, 1338, 1690, 1718
BslFI	GGGAC	2 cut(s)	491, 1388
BslI	CCNNNNNNNGG	5 cut(s)	675, 772, 1006, 1585, 1696
BsmAI	GTCTC	4 cut(s)	627, 787, 1147, 1485
BsmBI	CGTCTC	1 cut(s)	1485
BsmFI	GGGAC	2 cut(s)	491, 1388
BsmI	GAATGC	2 cut(s)	382, 914
BsnI	GGCC	2 cut(s)	356, 1411
BsoBI	CYCGRG	3 cut(s)	194, 1144, 1849
Bsp119I	TTCGAA	1 cut(s)	1353
Bsp1286I	GDGCHC	3 cut(s)	399, 695, 1231
Bsp1407I	TGTACA	1 cut(s)	243
Bsp1720I	GCTNAGC	1 cut(s)	1245
Bsp19I	CCATGG	1 cut(s)	1069
BspACI	CCGC	6 cut(s)	353, 392, 559, 730, 969, 1295
BspANI	GGCC	2 cut(s)	356, 1411
BspCNI	CTCAG	5 cut(s)	198, 820, 1237, 1482, 1495
BspFNI	CGCG	1 cut(s)	974
BspLI	GGNNCC	5 cut(s)	507, 598, 1347, 1613, 1856
BspMAI	CTGCAG	1 cut(s)	873
BspMI	ACCTGC	1 cut(s)	973
BspPI	GGATC	6 cut(s)	139, 591, 604, 757, 948, 1820
BspT104I	TTCGAA	1 cut(s)	1353
BspTI	CTTAAG	1 cut(s)	1750
BsrDI	GCAATG	3 cut(s)	253, 1320, 1815
BsrFI	RCCGGY	1 cut(s)	1689
BsrGI	TGTACA	1 cut(s)	243
BsrI	ACTGG	4 cut(s)	88, 278, 708, 766
BssAI	RCCGGY	1 cut(s)	1689
BssECI	CCNNGG	3 cut(s)	1069, 1230, 1369
BssNAI	GTATAC	1 cut(s)	1303
BssT1I	CCWWGG	2 cut(s)	1069, 1369
Bst1107I	GTATAC	1 cut(s)	1303
Bst2UI	CCWGG	1 cut(s)	1260
Bst4CI	ACNGT	1 cut(s)	1472
Bst6I	CTCTTC	3 cut(s)	162, 416, 1421
BstAFI	CTTAAG	1 cut(s)	1750
BstAUI	TGTACA	1 cut(s)	243
BstBI	TTCGAA	1 cut(s)	1353
BstC8I	GCNNGC	3 cut(s)	435, 1466, 1778
BstDEI	CTNAG	6 cut(s)	206, 828, 1245, 1482, 1490, 1841
BstDSI	CCRYGG	1 cut(s)	1069
BstF5I	GGATG	4 cut(s)	268, 1160, 1303, 1409
BstFNI	CGCG	1 cut(s)	974
BstH2I	RGCGCY	2 cut(s)	194, 1489
BstHHI	GCGC	6 cut(s)	193, 948, 974, 1170, 1488, 1521
BstMAI	GTCTC	4 cut(s)	627, 787, 1147, 1485
BstMCI	CGRYCG	2 cut(s)	135, 357
BstMWI	GCNNNNNNNGC	4 cut(s)	199, 1122, 1518, 1570
BstNI	CCWGG	1 cut(s)	1260
BstSCI	CCNGG	5 cut(s)	999, 1230, 1258, 1337, 1716
BstSFI	CTRYAG	1 cut(s)	869
BstSLI	GKGCMC	1 cut(s)	1231
BstUI	CGCG	1 cut(s)	974
BstV1I	GCAGC	8 cut(s)	201, 211, 627, 634, 855, 935, 1100, 1618
BstV2I	GAAGAC	1 cut(s)	182
BstX2I	RGATCY	7 cut(s)	144, 596, 762, 940, 1062, 1329, 1825
BstYI	RGATCY	7 cut(s)	144, 596, 762, 940, 1062, 1329, 1825
BstZ17I	GTATAC	1 cut(s)	1303
BstZI	CGGCCG	1 cut(s)	354
BsuRI	GGCC	2 cut(s)	356, 1411
BtgI	CCRYGG	1 cut(s)	1069
BtsCI	GGATG	4 cut(s)	268, 1160, 1303, 1409
BtsIMutI	CAGTG	3 cut(s)	95, 773, 1477
BveI	ACCTGC	1 cut(s)	973
Cac8I	GCNNGC	3 cut(s)	435, 1466, 1778
CaiI	CAGNNNCTG	2 cut(s)	205, 1489
CfoI	GCGC	6 cut(s)	193, 948, 974, 1170, 1488, 1521
Cfr10I	RCCGGY	1 cut(s)	1689
Cfr13I	GGNCC	1 cut(s)	505
CseI	GACGC	1 cut(s)	304
Csp6I	GTAC	5 cut(s)	244, 754, 874, 1275, 1835
CviAII	CATG	9 cut(s)	104, 259, 350, 790, 955, 1018, 1039, 1070, 1608
CviQI	GTAC	5 cut(s)	244, 754, 874, 1275, 1835
DdeI	CTNAG	6 cut(s)	206, 828, 1245, 1482, 1490, 1841
DriI	GACNNNNNGTC	1 cut(s)	1810
EaeI	YGGCCR	1 cut(s)	354
EagI	CGGCCG	1 cut(s)	354
Eam1104I	CTCTTC	3 cut(s)	162, 416, 1421
Eam1105I	GACNNNNNGTC	1 cut(s)	1810
EarI	CTCTTC	3 cut(s)	162, 416, 1421
Ecl136II	GAGCTC	1 cut(s)	693
EclXI	CGGCCG	1 cut(s)	354
Eco130I	CCWWGG	2 cut(s)	1069, 1369
Eco147I	AGGCCT	1 cut(s)	1411
Eco24I	GRGCYC	2 cut(s)	399, 695
Eco32I	GATATC	1 cut(s)	1015
Eco47I	GGWCC	1 cut(s)	505
Eco47III	AGCGCT	1 cut(s)	192
Eco52I	CGGCCG	1 cut(s)	354
Eco53kI	GAGCTC	1 cut(s)	693
Eco57I	CTGAAG	3 cut(s)	596, 1007, 1637
Eco88I	CYCGRG	3 cut(s)	194, 1144, 1849
EcoICRI	GAGCTC	1 cut(s)	693
EcoRI	GAATTC	1 cut(s)	823
EcoRII	CCWGG	1 cut(s)	1258
EcoRV	GATATC	1 cut(s)	1015
EcoT14I	CCWWGG	2 cut(s)	1069, 1369
EcoT38I	GRGCYC	2 cut(s)	399, 695
ErhI	CCWWGG	2 cut(s)	1069, 1369
Esp3I	CGTCTC	1 cut(s)	1485
FaeI	CATG	9 cut(s)	107, 262, 353, 793, 958, 1021, 1042, 1073, 1611
FalI	AAGNNNNNCTT	4 cut(s)	896, 928, 1334, 1366
FaqI	GGGAC	2 cut(s)	491, 1388
FatI	CATG	9 cut(s)	103, 258, 349, 789, 954, 1017, 1038, 1069, 1607
FauI	CCCGC	1 cut(s)	385
FauNDI	CATATG	1 cut(s)	1584
FbaI	TGATCA	2 cut(s)	75, 933
FblI	GTMKAC	1 cut(s)	1302
FokI	GGATG	4 cut(s)	275, 1147, 1310, 1416
FriOI	GRGCYC	2 cut(s)	399, 695
FspBI	CTAG	4 cut(s)	1056, 1241, 1694, 1713
FspI	TGCGCA	1 cut(s)	1169
GlaI	GCGC	6 cut(s)	192, 947, 973, 1169, 1487, 1520
GsuI	CTGGAG	1 cut(s)	402
HaeII	RGCGCY	2 cut(s)	194, 1489
HaeIII	GGCC	2 cut(s)	356, 1411
HapII	CCGG	6 cut(s)	13, 1001, 1232, 1338, 1690, 1718
HgaI	GACGC	1 cut(s)	304
HhaI	GCGC	6 cut(s)	193, 948, 974, 1170, 1488, 1521
Hin1II	CATG	9 cut(s)	107, 262, 353, 793, 958, 1021, 1042, 1073, 1611
Hin6I	GCGC	6 cut(s)	191, 946, 972, 1168, 1486, 1519
HinP1I	GCGC	6 cut(s)	191, 946, 972, 1168, 1486, 1519
HincII	GTYRAC	2 cut(s)	60, 1804
HindII	GTYRAC	2 cut(s)	60, 1804
HinfI	GANTC	9 cut(s)	70, 427, 707, 1358, 1604, 1738, 1745, 1784, 1847
HpaI	GTTAAC	1 cut(s)	60
HpaII	CCGG	6 cut(s)	13, 1001, 1232, 1338, 1690, 1718
HphI	GGTGA	2 cut(s)	592, 1422
Hpy166II	GTNNAC	4 cut(s)	60, 114, 1303, 1804
Hpy188III	TCNNGA	8 cut(s)	404, 508, 594, 646, 1333, 1615, 1726, 1742
Hpy8I	GTNNAC	4 cut(s)	60, 114, 1303, 1804
Hpy99I	CGWCG	1 cut(s)	320
HpyCH4III	ACNGT	1 cut(s)	1472
HpyCH4IV	ACGT	2 cut(s)	116, 1641
HpyF10VI	GCNNNNNNNGC	4 cut(s)	199, 1122, 1518, 1570
HpyF3I	CTNAG	6 cut(s)	206, 828, 1245, 1482, 1490, 1841
HpySE526I	ACGT	2 cut(s)	116, 1641
Hsp92II	CATG	9 cut(s)	107, 262, 353, 793, 958, 1021, 1042, 1073, 1611
HspAI	GCGC	6 cut(s)	191, 946, 972, 1168, 1486, 1519
Ksp22I	TGATCA	2 cut(s)	75, 933
KspAI	GTTAAC	1 cut(s)	60
LmnI	GCTCC	4 cut(s)	211, 238, 1110, 1860
Lsp1109I	GCAGC	8 cut(s)	201, 211, 627, 634, 855, 935, 1100, 1618
LweI	GCATC	4 cut(s)	253, 402, 1451, 1772
MaeI	CTAG	4 cut(s)	1056, 1241, 1694, 1713
MaeII	ACGT	2 cut(s)	116, 1641
MaeIII	GTNAC	1 cut(s)	1472
MflI	RGATCY	7 cut(s)	144, 596, 762, 940, 1062, 1329, 1825
MhlI	GDGCHC	3 cut(s)	399, 695, 1231
MluCI	AATT	4 cut(s)	8, 320, 823, 1447
MlyI	GAGTC	3 cut(s)	1613, 1747, 1841
MmeI	TCCRAC	4 cut(s)	172, 510, 642, 735
MroXI	GAANNNNTTC	1 cut(s)	912
MseI	TTAA	4 cut(s)	59, 671, 1451, 1751
MslI	CAYNNNNRTG	1 cut(s)	861
MspA1I	CMGCKG	1 cut(s)	1631
MspCI	CTTAAG	1 cut(s)	1750
MspI	CCGG	6 cut(s)	13, 1001, 1232, 1338, 1690, 1718
MspR9I	CCNGG	5 cut(s)	1001, 1232, 1260, 1339, 1718
Mva1269I	GAATGC	2 cut(s)	382, 914
MvaI	CCWGG	1 cut(s)	1260
MvnI	CGCG	1 cut(s)	974
MwoI	GCNNNNNNNGC	4 cut(s)	199, 1122, 1518, 1570
NciI	CCSGG	4 cut(s)	1001, 1232, 1339, 1718
NcoI	CCATGG	1 cut(s)	1069
NdeI	CATATG	1 cut(s)	1584
NlaIII	CATG	9 cut(s)	107, 262, 353, 793, 958, 1021, 1042, 1073, 1611
NlaIV	GGNNCC	5 cut(s)	507, 598, 1347, 1613, 1856
NmuCI	GTSAC	1 cut(s)	1472
NsbI	TGCGCA	1 cut(s)	1169
NspV	TTCGAA	1 cut(s)	1353
PaeR7I	CTCGAG	2 cut(s)	194, 1849
PceI	AGGCCT	1 cut(s)	1411
PctI	GAATGC	2 cut(s)	382, 914
PdmI	GAANNNNTTC	1 cut(s)	912
PfeI	GAWTC	6 cut(s)	70, 427, 707, 1358, 1745, 1784
PflFI	GACNNNGTC	1 cut(s)	314
PflMI	CCANNNNNTGG	1 cut(s)	772
Ple19I	CGATCG	1 cut(s)	135
PleI	GAGTC	3 cut(s)	1612, 1746, 1841
PpsI	GAGTC	3 cut(s)	1612, 1746, 1841
Psp124BI	GAGCTC	1 cut(s)	695
Psp6I	CCWGG	1 cut(s)	1258
PspGI	CCWGG	1 cut(s)	1258
PspN4I	GGNNCC	5 cut(s)	507, 598, 1347, 1613, 1856
PspPI	GGNCC	1 cut(s)	505
PspXI	VCTCGAGB	2 cut(s)	194, 1849
PstI	CTGCAG	1 cut(s)	873
PstNI	CAGNNNCTG	2 cut(s)	205, 1489
PsuI	RGATCY	7 cut(s)	144, 596, 762, 940, 1062, 1329, 1825
PsyI	GACNNNGTC	1 cut(s)	314
PvuI	CGATCG	1 cut(s)	135
PvuII	CAGCTG	1 cut(s)	1631
RsaI	GTAC	5 cut(s)	245, 755, 875, 1276, 1836
RsaNI	GTAC	5 cut(s)	244, 754, 874, 1275, 1835
RseI	CAYNNNNRTG	1 cut(s)	861
SacI	GAGCTC	1 cut(s)	695
SaqAI	TTAA	4 cut(s)	59, 671, 1451, 1751
Sau96I	GGNCC	1 cut(s)	505
SchI	GAGTC	3 cut(s)	1613, 1747, 1841
ScrFI	CCNGG	5 cut(s)	1001, 1232, 1260, 1339, 1718
SduI	GDGCHC	3 cut(s)	399, 695, 1231
SfaNI	GCATC	4 cut(s)	253, 402, 1451, 1772
SfcI	CTRYAG	1 cut(s)	869
Sfr274I	CTCGAG	2 cut(s)	194, 1849
SfuI	TTCGAA	1 cut(s)	1353
SinI	GGWCC	1 cut(s)	505
SlaI	CTCGAG	2 cut(s)	194, 1849
SmiMI	CAYNNNNRTG	1 cut(s)	861
SmlI	CTYRAG	5 cut(s)	194, 644, 1100, 1750, 1849
SmoI	CTYRAG	5 cut(s)	194, 644, 1100, 1750, 1849
Sse9I	AATT	4 cut(s)	8, 320, 823, 1447
SseBI	AGGCCT	1 cut(s)	1411
SsiI	CCGC	6 cut(s)	353, 392, 559, 730, 969, 1295
SspMI	CTAG	4 cut(s)	1056, 1241, 1694, 1713
SstI	GAGCTC	1 cut(s)	695
StuI	AGGCCT	1 cut(s)	1411
StyD4I	CCNGG	5 cut(s)	999, 1230, 1258, 1337, 1716
StyI	CCWWGG	2 cut(s)	1069, 1369
TaaI	ACNGT	1 cut(s)	1472
TaiI	ACGT	2 cut(s)	119, 1644
TaqI	TCGA	7 cut(s)	165, 195, 318, 399, 567, 1353, 1850
TasI	AATT	4 cut(s)	8, 320, 823, 1447
TatI	WGTACW	2 cut(s)	243, 753
TauI	GCSGC	3 cut(s)	356, 562, 972
TfiI	GAWTC	6 cut(s)	70, 427, 707, 1358, 1745, 1784
Tru1I	TTAA	4 cut(s)	59, 671, 1451, 1751
Tru9I	TTAA	4 cut(s)	59, 671, 1451, 1751
TscAI	CASTG	3 cut(s)	95, 773, 1477
TseFI	GTSAC	1 cut(s)	1472
TseI	GCWGC	8 cut(s)	199, 214, 622, 640, 868, 948, 1113, 1631
Tsp45I	GTSAC	1 cut(s)	1472
TspDTI	ATGAA	4 cut(s)	56, 320, 971, 1218
TspGWI	ACGGA	2 cut(s)	1153, 1301
TspRI	CASTG	3 cut(s)	95, 773, 1477
Tth111I	GACNNNGTC	1 cut(s)	314
Van91I	CCANNNNNTGG	1 cut(s)	772
Vha464I	CTTAAG	1 cut(s)	1750
VpaK11BI	GGWCC	1 cut(s)	505
XapI	RAATTY	2 cut(s)	320, 823
XcmI	CCANNNNNNNNNTGG	1 cut(s)	485
XhoI	CTCGAG	2 cut(s)	194, 1849
XmiI	GTMKAC	1 cut(s)	1302
XmnI	GAANNNNTTC	1 cut(s)	912
XspI	CTAG	4 cut(s)	1056, 1241, 1694, 1713

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.