MD06G1111300.v1.1

RING-H2 zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0015493
Physical Location & Seq
Reverse (-)
25004478 .. 25007139
2662 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1111300.v1.1.491

Sequence Viewer

Length: 804 bp
ATGGCCAGTCTTCCTCAAACTCCAAACAGCACTAGTAATTCTCACCTTTATCCTCAAGCACTCCAACTTAAACTTTACCAGGCCTTCATATTCTCGATTCCTATCCTTTTTTCCATCATCCTTTTTCTCCTCTTCTACTTGTTCTACCTCAAAAGAAGGGCTTCCGCTCTCTTATCATCTTCTCAACCAGTTCTTCCAAGGAGTAATAACTATAATCTTCAAGCTGCACCTACCACATATTATGTTTCCACTGATTGTCCGGTGGGTTTGAAGGGGGAGCTCAAGGAGAAGCTTCAAACAATTTTATTTGATGAAGAACAAAGGAAAAAGGATTCACAATGCTGTGTTTGCTTGGGGGAATTCGAAATGGAAGAAGAGTTGCTGCAAGTGCGATCATGCAAACACGTGTTTCATGTTGATTGCATACATCACTGGCTGCATAACAACACAACTTGCCCACTTTGCAGATGCACTGTGATTCCAATCAGTACCAAACTTGATAGTCCTGCACCACCACCTGCAGCTGCTGGTGTGTCCGACCCAGTAGTACAACAGCAGCACCATAGCATAATAATATCCCATCAGAGTCCAGGTATTATTGTATTGTTGGACCAACAAAATCAGCAACAACAGCATCAAACGGGCAATAATGCTATTTCTGGTGTAACAACACCAACTGAAGAAGGTTCATCAAGTGACCAAAGTAGTTCGATTTTAAGGGACTCTGGGTGTTTACCTGGGTACTATACTAATCAAGATTTGAGAGAACCAGTCGTTGTCTATATCCAAACTCATGATTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

268

Amino Acids

29.83

Weight (kDa)

5.96

Isoelectric Point (pI)

67.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 113 - 156 3.6e-12 Ring finger domain
zf-RING_11 PF17123 113 - 142 4.9e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 113 - 155 9.8e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 114 - 156 1.2e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015493)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24015
fragaria_vesca FvH4_5g11670
malus_domestica MD06G1111300.v1.1 MD14G1132500.v1.1
prunus_persica Prupe.5G124100_v2.0.a1
pyrus_communis pycom06g10430
rosa_chinensis RchiOBHm_Chr7g0186121
rosa_laevigata RLG00000004862
rosa_multiflora Rmu_sc0000194.1_g000030
rosa_roxburghii Rroxscaffold_3G00268410
rosa_rugosa Rorug06G0472200
rosa_samantha Rh7AG069400 Rh7BG085400 Rh7CG078700 Rh7DG078800
rosa_wichuraiana Rw7G006550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 526
Acc36I ACCTGC 1 cut(s) 526
AccBSI CCGCTC 1 cut(s) 167
AciI CCGC 1 cut(s) 165
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 359
AcuI CTGAAG 1 cut(s) 699
AcvI CACGTG 1 cut(s) 406
AfaI GTAC 3 cut(s) 490, 549, 743
AflIII ACRYGT 2 cut(s) 403, 405
AgsI TTSAA 3 cut(s) 221, 271, 296
AhlI ACTAGT 1 cut(s) 32
AjnI CCWGG 3 cut(s) 78, 589, 736
AluBI AGCT 4 cut(s) 224, 280, 292, 524
AluI AGCT 4 cut(s) 224, 280, 292, 524
Alw21I GWGCWC 1 cut(s) 282
AlwNI CAGNNNCTG 1 cut(s) 527
AoxI GGCC 2 cut(s) 3, 81
ApeKI GCWGC 6 cut(s) 224, 382, 436, 521, 524, 556
ApoI RAATTY 1 cut(s) 359
Asp700I GAANNNNTTC 1 cut(s) 160
AspS9I GGNCC 1 cut(s) 610
AsuHPI GGTGA 1 cut(s) 35
AsuII TTCGAA 1 cut(s) 363
AvaII GGWCC 1 cut(s) 610
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 282
BbrPI CACGTG 1 cut(s) 406
BbsI GAAGAC 1 cut(s) 2
Bbv12I GWGCWC 1 cut(s) 282
BbvI GCAGC 6 cut(s) 211, 369, 423, 511, 533, 568
BccI CCATC 2 cut(s) 122, 588
BciT130I CCWGG 3 cut(s) 80, 591, 738
BcuI ACTAGT 1 cut(s) 32
BfaI CTAG 1 cut(s) 33
BfmI CTRYAG 1 cut(s) 519
BfuAI ACCTGC 1 cut(s) 526
BisI GCNGC 6 cut(s) 225, 383, 437, 522, 525, 557
BlsI GCNGC 6 cut(s) 226, 384, 438, 523, 526, 558
Bme1390I CCNGG 3 cut(s) 80, 591, 738
Bme18I GGWCC 1 cut(s) 610
BmgT120I GGNCC 1 cut(s) 610
BmrFI CCNGG 3 cut(s) 80, 591, 738
BmrI ACTGGG 1 cut(s) 536
BmsI GCATC 2 cut(s) 458, 643
BmuI ACTGGG 1 cut(s) 536
BpiI GAAGAC 1 cut(s) 2
Bpu14I TTCGAA 1 cut(s) 363
BpuEI CTTGAG 2 cut(s) 39, 266
BsaAI YACGTR 1 cut(s) 406
BsaBI GATNNNNATC 2 cut(s) 101, 482
BsaJI CCNNGG 2 cut(s) 197, 737
BsaWI WCCGGW 1 cut(s) 259
Bse1I ACTGG 5 cut(s) 6, 188, 437, 542, 770
Bse8I GATNNNNATC 2 cut(s) 101, 482
BseBI CCWGG 3 cut(s) 80, 591, 738
BseDI CCNNGG 2 cut(s) 197, 737
BseGI GGATG 1 cut(s) 117
BseJI GATNNNNATC 2 cut(s) 101, 482
BseNI ACTGG 5 cut(s) 6, 188, 437, 542, 770
BseRI GAGGAG 1 cut(s) 119
BseXI GCAGC 6 cut(s) 211, 369, 423, 511, 533, 568
BsgI GTGCAG 2 cut(s) 210, 492
BshFI GGCC 2 cut(s) 5, 83
BsiHKAI GWGCWC 1 cut(s) 282
BsiSI CCGG 1 cut(s) 260
BslFI GGGAC 1 cut(s) 734
BsmFI GGGAC 1 cut(s) 734
BsnI GGCC 2 cut(s) 5, 83
Bsp119I TTCGAA 1 cut(s) 363
Bsp1286I GDGCHC 1 cut(s) 282
Bsp143I GATC 1 cut(s) 392
BspACI CCGC 1 cut(s) 165
BspANI GGCC 2 cut(s) 5, 83
BspHI TCATGA 1 cut(s) 793
BspMAI CTGCAG 1 cut(s) 523
BspMI ACCTGC 1 cut(s) 526
BspT104I TTCGAA 1 cut(s) 363
BsrBI CCGCTC 1 cut(s) 167
BsrI ACTGG 5 cut(s) 6, 188, 437, 542, 770
BssECI CCNNGG 2 cut(s) 197, 737
BssMI GATC 1 cut(s) 392
BssT1I CCWWGG 1 cut(s) 197
Bst2UI CCWGG 3 cut(s) 80, 591, 738
Bst4CI ACNGT 1 cut(s) 475
Bst6I CTCTTC 2 cut(s) 137, 369
BstBAI YACGTR 1 cut(s) 406
BstBI TTCGAA 1 cut(s) 363
BstF5I GGATG 1 cut(s) 117
BstKTI GATC 1 cut(s) 395
BstMBI GATC 1 cut(s) 392
BstMWI GCNNNNNNNGC 4 cut(s) 348, 388, 462, 631
BstNI CCWGG 3 cut(s) 80, 591, 738
BstSCI CCNGG 3 cut(s) 78, 589, 736
BstSFI CTRYAG 1 cut(s) 519
BstV1I GCAGC 6 cut(s) 211, 369, 423, 511, 533, 568
BstV2I GAAGAC 1 cut(s) 2
BsuRI GGCC 2 cut(s) 5, 83
BtsCI GGATG 1 cut(s) 117
BtsIMutI CAGTG 3 cut(s) 249, 430, 471
BveI ACCTGC 1 cut(s) 526
CaiI CAGNNNCTG 1 cut(s) 527
CciI TCATGA 1 cut(s) 793
Cfr13I GGNCC 1 cut(s) 610
Csp6I GTAC 3 cut(s) 489, 548, 742
CviAII CATG 3 cut(s) 396, 413, 794
CviJI RGCY 8 cut(s) 5, 83, 161, 224, 280, 292, 436, 524
CviKI_1 RGCY 8 cut(s) 5, 83, 161, 224, 280, 292, 436, 524
CviQI GTAC 3 cut(s) 489, 548, 742
DpnI GATC 1 cut(s) 394
DpnII GATC 1 cut(s) 392
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 137, 369
EarI CTCTTC 2 cut(s) 137, 369
Ecl136II GAGCTC 1 cut(s) 280
Eco130I CCWWGG 1 cut(s) 197
Eco147I AGGCCT 1 cut(s) 83
Eco24I GRGCYC 1 cut(s) 282
Eco47I GGWCC 1 cut(s) 610
Eco53kI GAGCTC 1 cut(s) 280
Eco57I CTGAAG 1 cut(s) 699
Eco72I CACGTG 1 cut(s) 406
EcoICRI GAGCTC 1 cut(s) 280
EcoRI GAATTC 1 cut(s) 359
EcoRII CCWGG 3 cut(s) 78, 589, 736
EcoT14I CCWWGG 1 cut(s) 197
EcoT38I GRGCYC 1 cut(s) 282
ErhI CCWWGG 1 cut(s) 197
FaeI CATG 3 cut(s) 399, 416, 797
FalI AAGNNNNNCTT 2 cut(s) 145, 177
FaqI GGGAC 1 cut(s) 734
FatI CATG 3 cut(s) 395, 412, 793
Fnu4HI GCNGC 6 cut(s) 225, 383, 437, 522, 525, 557
FokI GGATG 1 cut(s) 104
FriOI GRGCYC 1 cut(s) 282
Fsp4HI GCNGC 6 cut(s) 225, 383, 437, 522, 525, 557
FspBI CTAG 1 cut(s) 33
GluI GCNGC 6 cut(s) 225, 383, 437, 522, 525, 557
HaeIII GGCC 2 cut(s) 5, 83
HapII CCGG 1 cut(s) 260
Hin1II CATG 3 cut(s) 399, 416, 797
HindIII AAGCTT 1 cut(s) 290
HinfI GANTC 6 cut(s) 97, 332, 478, 586, 722, 797
HpaII CCGG 1 cut(s) 260
HphI GGTGA 1 cut(s) 35
Hpy166II GTNNAC 1 cut(s) 734
Hpy188I TCNGA 2 cut(s) 538, 585
Hpy188III TCNNGA 3 cut(s) 94, 755, 794
Hpy8I GTNNAC 1 cut(s) 734
HpyAV CCTTC 4 cut(s) 94, 150, 265, 677
HpyCH4III ACNGT 1 cut(s) 475
HpyCH4IV ACGT 1 cut(s) 405
HpyCH4V TGCA 9 cut(s) 227, 385, 399, 423, 439, 465, 471, 509, 521
HpyF10VI GCNNNNNNNGC 4 cut(s) 348, 388, 462, 631
HpySE526I ACGT 1 cut(s) 405
Hsp92II CATG 3 cut(s) 399, 416, 797
Kzo9I GATC 1 cut(s) 392
LmnI GCTCC 1 cut(s) 277
Lsp1109I GCAGC 6 cut(s) 211, 369, 423, 511, 533, 568
LweI GCATC 2 cut(s) 458, 643
MaeI CTAG 1 cut(s) 33
MaeII ACGT 1 cut(s) 405
MaeIII GTNAC 2 cut(s) 664, 695
MalI GATC 1 cut(s) 394
MbiI CCGCTC 1 cut(s) 167
MboI GATC 1 cut(s) 392
MboII GAAGA 8 cut(s) 124, 171, 185, 209, 326, 383, 386, 692
MhlI GDGCHC 1 cut(s) 282
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 37, 300, 359
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 595, 716
MmeI TCCRAC 3 cut(s) 88, 561, 588
MnlI CCTC 4 cut(s) 24, 63, 140, 158
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 160
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 69, 716
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 524
MspI CCGG 1 cut(s) 260
MspR9I CCNGG 3 cut(s) 80, 591, 738
MvaI CCWGG 3 cut(s) 80, 591, 738
MwoI GCNNNNNNNGC 4 cut(s) 348, 388, 462, 631
NdeII GATC 1 cut(s) 392
NlaIII CATG 3 cut(s) 399, 416, 797
NmuCI GTSAC 1 cut(s) 695
NspV TTCGAA 1 cut(s) 363
PagI TCATGA 1 cut(s) 793
PaqCI CACCTGC 1 cut(s) 526
PceI AGGCCT 1 cut(s) 83
PdmI GAANNNNTTC 1 cut(s) 160
PfeI GAWTC 4 cut(s) 97, 332, 478, 797
PkrI GCNGC 6 cut(s) 226, 384, 438, 523, 526, 558
PleI GAGTC 2 cut(s) 594, 716
PmaCI CACGTG 1 cut(s) 406
PmlI CACGTG 1 cut(s) 406
PpsI GAGTC 2 cut(s) 594, 716
Ppu21I YACGTR 1 cut(s) 406
Psp124BI GAGCTC 1 cut(s) 282
Psp6I CCWGG 3 cut(s) 78, 589, 736
PspCI CACGTG 1 cut(s) 406
PspGI CCWGG 3 cut(s) 78, 589, 736
PspPI GGNCC 1 cut(s) 610
PstI CTGCAG 1 cut(s) 523
PstNI CAGNNNCTG 1 cut(s) 527
PvuII CAGCTG 1 cut(s) 524
RsaI GTAC 3 cut(s) 490, 549, 743
RsaNI GTAC 3 cut(s) 489, 548, 742
SacI GAGCTC 1 cut(s) 282
SaqAI TTAA 2 cut(s) 69, 716
SatI GCNGC 6 cut(s) 225, 383, 437, 522, 525, 557
Sau3AI GATC 1 cut(s) 392
Sau96I GGNCC 1 cut(s) 610
SchI GAGTC 2 cut(s) 595, 716
ScrFI CCNGG 3 cut(s) 80, 591, 738
SduI GDGCHC 1 cut(s) 282
SfaNI GCATC 2 cut(s) 458, 643
SfcI CTRYAG 1 cut(s) 519
SfuI TTCGAA 1 cut(s) 363
SinI GGWCC 1 cut(s) 610
SmlI CTYRAG 2 cut(s) 54, 281
SmoI CTYRAG 2 cut(s) 54, 281
SpeI ACTAGT 1 cut(s) 32
Sse9I AATT 3 cut(s) 37, 300, 359
SseBI AGGCCT 1 cut(s) 83
SsiI CCGC 1 cut(s) 165
SspMI CTAG 1 cut(s) 33
SstI GAGCTC 1 cut(s) 282
StuI AGGCCT 1 cut(s) 83
StyD4I CCNGG 3 cut(s) 78, 589, 736
StyI CCWWGG 1 cut(s) 197
TaaI ACNGT 1 cut(s) 475
TaiI ACGT 1 cut(s) 408
TaqI TCGA 3 cut(s) 95, 363, 710
TasI AATT 3 cut(s) 37, 300, 359
TatI WGTACW 1 cut(s) 547
TfiI GAWTC 4 cut(s) 97, 332, 478, 797
Tru1I TTAA 2 cut(s) 69, 716
Tru9I TTAA 2 cut(s) 69, 716
TscAI CASTG 3 cut(s) 256, 437, 478
TseFI GTSAC 1 cut(s) 695
TseI GCWGC 6 cut(s) 224, 382, 436, 521, 524, 556
Tsp45I GTSAC 1 cut(s) 695
TspDTI ATGAA 5 cut(s) 76, 327, 401, 678, 789
TspRI CASTG 3 cut(s) 256, 437, 478
VpaK11BI GGWCC 1 cut(s) 610
XapI RAATTY 1 cut(s) 359
XmnI GAANNNNTTC 1 cut(s) 160
XspI CTAG 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.