Rw7G006550

RING-H2 zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0015493
Physical Location & Seq
Reverse (-)
5604151 .. 5606583
2433 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G006550.1

Sequence Viewer

Length: 831 bp
ATGGGTCTTCCTGATCAAACTCAAATGACCAGTTCTACTACTACTTCTCAATCTCATCTTTATCCTCAAGCACTCCAACTCAAACTTTACCAGGCCTTCATATTCTCGATTCCTATTCTTTTCTCCATCATTCTCTTTCTGTTGTTCTACTTGTTCTACCTCAAAAGGAGGGCTTCCACACTTTCATCTTCTCCTCCAATTCTCATTCGAAGAAGAAGCCATAATCAAGCTTCAGCACCTTCACCATATGCTTCCTCACCTTGTCCAATGGATTTGAAGGGAGAGCTCAAAGAGAAGCTTCGGATCATTCTTTTTGATGAAGAACTGAGGAAAAAGGATTCCCAATGTTGTGTTTGCTTGGGGGAATTCGAAATGGAAGAGGAGCTACTGCAGGTTCAATCGTGCAAACATGTCTTCCATGTCGATTGCATACATCACTGGCTGCACAACAATACAACCTGCCCCCTTTGCAGATGCTCGGTGATTTCGATTAGTATGAGTACCAAACTCGATAGTAATCCGGCACCAGTAACTGTTAGTACCACCGTGTCAACGGAGCCAGCTGCACAACAACTCCAGCAGCACCATAGCATAGTAGTCTCCCACCACAGTCCTGGGATAGTATCGTTGGAGCTACGACAACACCAGGAACAACAGCAACAGATGGACAATTACAATTACGTAGCTTCAAATGTGACAGCACCGGTTGGAGAAGAGTCATCATCAATCACGGGGACTGGTACTGGTTCAACTTTAAGGGACTCTGGGTGTTCTAGTACTAGTGATCGAGATTTGGGAGACTCGGTTACTATTCATATCCGAGACGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

276

Amino Acids

30.78

Weight (kDa)

6.21

Isoelectric Point (pI)

69.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 115 - 158 3.1e-12 Ring finger domain
zf-RING_11 PF17123 115 - 144 6.5e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 115 - 157 1.1e-06 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 116 - 158 2e-08 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015493)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24015
fragaria_vesca FvH4_5g11670
malus_domestica MD06G1111300.v1.1 MD14G1132500.v1.1
prunus_persica Prupe.5G124100_v2.0.a1
pyrus_communis pycom06g10430
rosa_chinensis RchiOBHm_Chr7g0186121
rosa_laevigata RLG00000004862
rosa_multiflora Rmu_sc0000194.1_g000030
rosa_roxburghii Rroxscaffold_3G00268410
rosa_rugosa Rorug06G0472200
rosa_samantha Rh7AG069400 Rh7BG085400 Rh7CG078700 Rh7DG078800
rosa_wichuraiana Rw7G006550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 382, 467
AccB1I GGYRCC 1 cut(s) 523
AclWI GGATC 1 cut(s) 311
AcsI RAATTY 1 cut(s) 365
AcuI CTGAAG 1 cut(s) 216
AfaI GTAC 4 cut(s) 502, 541, 742, 778
AflIII ACRYGT 1 cut(s) 409
AgeI ACCGGT 1 cut(s) 703
AgsI TTSAA 4 cut(s) 277, 398, 690, 750
AhlI ACTAGT 1 cut(s) 779
AjnI CCWGG 3 cut(s) 90, 613, 645
AluBI AGCT 7 cut(s) 230, 286, 298, 385, 563, 634, 686
AluI AGCT 7 cut(s) 230, 286, 298, 385, 563, 634, 686
Alw21I GWGCWC 1 cut(s) 288
Alw26I GTCTC 3 cut(s) 604, 792, 816
AlwI GGATC 1 cut(s) 311
AlwNI CAGNNNCTG 1 cut(s) 533
AoxI GGCC 1 cut(s) 93
ApeKI GCWGC 3 cut(s) 442, 563, 580
ApoI RAATTY 1 cut(s) 365
AsiGI ACCGGT 1 cut(s) 703
AsuHPI GGTGA 3 cut(s) 234, 249, 493
AsuII TTCGAA 2 cut(s) 208, 369
BanI GGYRCC 1 cut(s) 523
BanII GRGCYC 1 cut(s) 288
BarI GAAGNNNNNNTAC 2 cut(s) 369, 401
BbsI GAAGAC 1 cut(s) 406
Bbv12I GWGCWC 1 cut(s) 288
BbvI GCAGC 3 cut(s) 429, 550, 592
BccI CCATC 2 cut(s) 134, 658
BciT130I CCWGG 3 cut(s) 92, 615, 647
BclI TGATCA 1 cut(s) 13
BcoDI GTCTC 3 cut(s) 604, 792, 816
BcuI ACTAGT 1 cut(s) 779
BfaI CTAG 2 cut(s) 774, 780
BfmI CTRYAG 1 cut(s) 389
BfuAI ACCTGC 2 cut(s) 382, 467
BisI GCNGC 3 cut(s) 443, 564, 581
BlsI GCNGC 3 cut(s) 444, 565, 582
BmcAI AGTACT 1 cut(s) 778
Bme1390I CCNGG 3 cut(s) 92, 615, 647
BmiI GGNNCC 2 cut(s) 525, 558
BmrFI CCNGG 3 cut(s) 92, 615, 647
BmsI GCATC 1 cut(s) 464
BpiI GAAGAC 1 cut(s) 406
BpmI CTGGAG 1 cut(s) 560
Bpu14I TTCGAA 2 cut(s) 208, 369
BpuEI CTTGAG 1 cut(s) 51
BsaAI YACGTR 1 cut(s) 682
BsaBI GATNNNNATC 1 cut(s) 516
BsaJI CCNNGG 1 cut(s) 614
BsaWI WCCGGW 1 cut(s) 703
BsaXI ACNNNNNCTCC 4 cut(s) 558, 588, 789, 819
Bse118I RCCGGY 1 cut(s) 703
Bse1I ACTGG 5 cut(s) 30, 443, 527, 742, 748
Bse8I GATNNNNATC 1 cut(s) 516
BseBI CCWGG 3 cut(s) 92, 615, 647
BseDI CCNNGG 1 cut(s) 614
BseJI GATNNNNATC 1 cut(s) 516
BseMII CTCAG 1 cut(s) 317
BseNI ACTGG 5 cut(s) 30, 443, 527, 742, 748
BseRI GAGGAG 2 cut(s) 183, 395
BseXI GCAGC 3 cut(s) 429, 550, 592
BsgI GTGCAG 2 cut(s) 428, 549
BshFI GGCC 1 cut(s) 95
BshNI GGYRCC 1 cut(s) 523
BshTI ACCGGT 1 cut(s) 703
BsiHKAI GWGCWC 1 cut(s) 288
BsiSI CCGG 2 cut(s) 521, 704
BslFI GGGAC 2 cut(s) 748, 773
BsmAI GTCTC 3 cut(s) 604, 792, 816
BsmBI CGTCTC 1 cut(s) 816
BsmFI GGGAC 2 cut(s) 748, 773
BsnI GGCC 1 cut(s) 95
Bsp119I TTCGAA 2 cut(s) 208, 369
Bsp1286I GDGCHC 1 cut(s) 288
Bsp143I GATC 3 cut(s) 13, 303, 784
BspANI GGCC 1 cut(s) 95
BspCNI CTCAG 1 cut(s) 318
BspLI GGNNCC 2 cut(s) 525, 558
BspMAI CTGCAG 1 cut(s) 393
BspMI ACCTGC 2 cut(s) 382, 467
BspPI GGATC 1 cut(s) 311
BspT104I TTCGAA 2 cut(s) 208, 369
BspT107I GGYRCC 1 cut(s) 523
BsrFI RCCGGY 1 cut(s) 703
BsrI ACTGG 5 cut(s) 30, 443, 527, 742, 748
BssAI RCCGGY 1 cut(s) 703
BssECI CCNNGG 1 cut(s) 614
BssMI GATC 3 cut(s) 13, 303, 784
Bst2UI CCWGG 3 cut(s) 92, 615, 647
Bst4CI ACNGT 3 cut(s) 535, 547, 611
Bst6I CTCTTC 2 cut(s) 372, 708
BstBAI YACGTR 1 cut(s) 682
BstBI TTCGAA 2 cut(s) 208, 369
BstC8I GCNNGC 1 cut(s) 561
BstDEI CTNAG 1 cut(s) 326
BstKTI GATC 3 cut(s) 16, 306, 787
BstMAI GTCTC 3 cut(s) 604, 792, 816
BstMBI GATC 3 cut(s) 13, 303, 784
BstMWI GCNNNNNNNGC 1 cut(s) 468
BstNI CCWGG 3 cut(s) 92, 615, 647
BstNSI RCATGY 1 cut(s) 413
BstSCI CCNGG 3 cut(s) 90, 613, 645
BstSFI CTRYAG 1 cut(s) 389
BstSNI TACGTA 1 cut(s) 682
BstV1I GCAGC 3 cut(s) 429, 550, 592
BstV2I GAAGAC 1 cut(s) 406
BstXI CCANNNNNNTGG 1 cut(s) 614
BsuRI GGCC 1 cut(s) 95
BtsIMutI CAGTG 1 cut(s) 436
BveI ACCTGC 2 cut(s) 382, 467
Cac8I GCNNGC 1 cut(s) 561
CaiI CAGNNNCTG 1 cut(s) 533
Cfr10I RCCGGY 1 cut(s) 703
Csp6I GTAC 4 cut(s) 501, 540, 741, 777
CspAI ACCGGT 1 cut(s) 703
CviAII CATG 2 cut(s) 410, 419
CviQI GTAC 4 cut(s) 501, 540, 741, 777
DdeI CTNAG 1 cut(s) 326
DpnI GATC 3 cut(s) 15, 305, 786
DpnII GATC 3 cut(s) 13, 303, 784
Eam1104I CTCTTC 2 cut(s) 372, 708
EarI CTCTTC 2 cut(s) 372, 708
Ecl136II GAGCTC 1 cut(s) 286
Eco105I TACGTA 1 cut(s) 682
Eco147I AGGCCT 1 cut(s) 95
Eco24I GRGCYC 1 cut(s) 288
Eco53kI GAGCTC 1 cut(s) 286
Eco57I CTGAAG 1 cut(s) 216
EcoICRI GAGCTC 1 cut(s) 286
EcoRI GAATTC 1 cut(s) 365
EcoRII CCWGG 3 cut(s) 90, 613, 645
EcoT38I GRGCYC 1 cut(s) 288
Esp3I CGTCTC 1 cut(s) 816
FaeI CATG 2 cut(s) 413, 422
FalI AAGNNNNNCTT 4 cut(s) 157, 189, 282, 314
FaqI GGGAC 2 cut(s) 748, 773
FatI CATG 2 cut(s) 409, 418
FauNDI CATATG 1 cut(s) 247
FbaI TGATCA 1 cut(s) 13
Fnu4HI GCNGC 3 cut(s) 443, 564, 581
FriOI GRGCYC 1 cut(s) 288
Fsp4HI GCNGC 3 cut(s) 443, 564, 581
FspBI CTAG 2 cut(s) 774, 780
GluI GCNGC 3 cut(s) 443, 564, 581
GsuI CTGGAG 1 cut(s) 560
HaeIII GGCC 1 cut(s) 95
HapII CCGG 2 cut(s) 521, 704
Hin1II CATG 2 cut(s) 413, 422
HincII GTYRAC 1 cut(s) 552
HindII GTYRAC 1 cut(s) 552
HindIII AAGCTT 2 cut(s) 228, 296
HinfI GANTC 5 cut(s) 109, 338, 716, 761, 800
HpaII CCGG 2 cut(s) 521, 704
HphI GGTGA 3 cut(s) 234, 249, 493
Hpy166II GTNNAC 1 cut(s) 552
Hpy188I TCNGA 2 cut(s) 303, 821
Hpy188III TCNNGA 3 cut(s) 11, 106, 788
Hpy8I GTNNAC 1 cut(s) 552
HpyAV CCTTC 3 cut(s) 106, 249, 271
HpyCH4III ACNGT 3 cut(s) 535, 547, 611
HpyCH4IV ACGT 2 cut(s) 681, 825
HpyCH4V TGCA 6 cut(s) 391, 405, 429, 445, 471, 566
HpyF10VI GCNNNNNNNGC 1 cut(s) 468
HpyF3I CTNAG 1 cut(s) 326
HpySE526I ACGT 2 cut(s) 681, 825
Hsp92II CATG 2 cut(s) 413, 422
Ksp22I TGATCA 1 cut(s) 13
Kzo9I GATC 3 cut(s) 13, 303, 784
LmnI GCTCC 3 cut(s) 382, 556, 631
Lsp1109I GCAGC 3 cut(s) 429, 550, 592
LweI GCATC 1 cut(s) 464
MaeI CTAG 2 cut(s) 774, 780
MaeII ACGT 2 cut(s) 681, 825
MaeIII GTNAC 3 cut(s) 529, 694, 805
MalI GATC 3 cut(s) 15, 305, 786
MboI GATC 3 cut(s) 13, 303, 784
MboII GAAGA 7 cut(s) 180, 222, 225, 332, 389, 406, 725
MhlI GDGCHC 1 cut(s) 288
MluCI AATT 4 cut(s) 198, 365, 670, 676
MlyI GAGTC 3 cut(s) 725, 755, 794
MmeI TCCRAC 3 cut(s) 100, 609, 688
MnlI CCTC 7 cut(s) 75, 162, 170, 204, 265, 321, 373
MseI TTAA 2 cut(s) 755, 829
MspA1I CMGCKG 1 cut(s) 563
MspI CCGG 2 cut(s) 521, 704
MspR9I CCNGG 3 cut(s) 92, 615, 647
MvaI CCWGG 3 cut(s) 92, 615, 647
MwoI GCNNNNNNNGC 1 cut(s) 468
NdeI CATATG 1 cut(s) 247
NdeII GATC 3 cut(s) 13, 303, 784
NlaIII CATG 2 cut(s) 413, 422
NlaIV GGNNCC 2 cut(s) 525, 558
NmuCI GTSAC 1 cut(s) 694
NspI RCATGY 1 cut(s) 413
NspV TTCGAA 2 cut(s) 208, 369
PceI AGGCCT 1 cut(s) 95
PciI ACATGT 1 cut(s) 409
PcsI WCGNNNNNNNCGW 1 cut(s) 485
PfeI GAWTC 2 cut(s) 109, 338
PinAI ACCGGT 1 cut(s) 703
PkrI GCNGC 3 cut(s) 444, 565, 582
PleI GAGTC 3 cut(s) 724, 755, 794
PpsI GAGTC 3 cut(s) 724, 755, 794
Ppu21I YACGTR 1 cut(s) 682
PscI ACATGT 1 cut(s) 409
Psp124BI GAGCTC 1 cut(s) 288
Psp6I CCWGG 3 cut(s) 90, 613, 645
PspGI CCWGG 3 cut(s) 90, 613, 645
PspN4I GGNNCC 2 cut(s) 525, 558
PstI CTGCAG 1 cut(s) 393
PstNI CAGNNNCTG 1 cut(s) 533
PvuII CAGCTG 1 cut(s) 563
RsaI GTAC 4 cut(s) 502, 541, 742, 778
RsaNI GTAC 4 cut(s) 501, 540, 741, 777
SacI GAGCTC 1 cut(s) 288
SaqAI TTAA 2 cut(s) 755, 829
SatI GCNGC 3 cut(s) 443, 564, 581
Sau3AI GATC 3 cut(s) 13, 303, 784
ScaI AGTACT 1 cut(s) 778
SchI GAGTC 3 cut(s) 725, 755, 794
ScrFI CCNGG 3 cut(s) 92, 615, 647
SduI GDGCHC 1 cut(s) 288
SfaNI GCATC 1 cut(s) 464
SfcI CTRYAG 1 cut(s) 389
SfuI TTCGAA 2 cut(s) 208, 369
SmlI CTYRAG 1 cut(s) 66
SmoI CTYRAG 1 cut(s) 66
SnaBI TACGTA 1 cut(s) 682
SpeI ACTAGT 1 cut(s) 779
Sse9I AATT 4 cut(s) 198, 365, 670, 676
SseBI AGGCCT 1 cut(s) 95
SspMI CTAG 2 cut(s) 774, 780
SstI GAGCTC 1 cut(s) 288
StuI AGGCCT 1 cut(s) 95
StyD4I CCNGG 3 cut(s) 90, 613, 645
TaaI ACNGT 3 cut(s) 535, 547, 611
TaiI ACGT 2 cut(s) 684, 828
TaqI TCGA 7 cut(s) 107, 208, 369, 423, 488, 510, 787
TasI AATT 4 cut(s) 198, 365, 670, 676
TatI WGTACW 1 cut(s) 776
TfiI GAWTC 2 cut(s) 109, 338
Tru1I TTAA 2 cut(s) 755, 829
Tru9I TTAA 2 cut(s) 755, 829
TscAI CASTG 1 cut(s) 443
TseFI GTSAC 1 cut(s) 694
TseI GCWGC 3 cut(s) 442, 563, 580
Tsp45I GTSAC 1 cut(s) 694
TspDTI ATGAA 4 cut(s) 88, 174, 333, 803
TspGWI ACGGA 1 cut(s) 569
TspRI CASTG 1 cut(s) 443
XapI RAATTY 1 cut(s) 365
XceI RCATGY 1 cut(s) 413
XcmI CCANNNNNNNNNTGG 1 cut(s) 611
XspI CTAG 2 cut(s) 774, 780
ZrmI AGTACT 1 cut(s) 778
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.