MD14G1132500.v1.1

RING-H2 zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0015493
Physical Location & Seq
Reverse (-)
21043175 .. 21045718
2544 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1132500.v1.1.491

Sequence Viewer

Length: 801 bp
ATGGGCAGTCTTCCTCAAACACCAAACAGTACTAGTAATCCTAACCTTTATCCTCAAGCACTCCAACTTAAACTTTACCACGCCTTCATATTCTCGATTCCTATCCTTTTCTCCATCATTCTTTTTCTTCTCTTCTACTTGTTCTACCTCAAAAGAAGGGCTTCCGCTCTCTTATCTTCTTCTCAACCAGTTTTTCCAAGGAGTAATTATAATCTTCAAGCTGCACCTACCAGATACCATGTTTCCACTGCTTGTCCGGTGGGTTTGAAGGGGGAGCTCAAGGAGAAGCTTCAAACAATTTTATTTGATGAAGAACAAAGGAAAAAGGATTCACAATGCTGTGTTTGCTTGAGTGAATTCGAAGTGGAAGAGGAATTACTGCAAGTGCTATCATGCAAACACGTGTTTCATGTTGATTGTATACATCACTGGCTGCATAACAACACAACTTGCCCACTTTGCAGATGCTCTGTGATTCCCATCAGTACCATACTCGACAGTCCCACACCACCTGCAGCTGCTAGGGTGTTCGACCCAGTAATACAAGAGCAGCACCATAGCATAATAATCTCCCATCAGAGTCCAGTGAGTATTGTATTATTGGACCAACAAAATCAGCAACAACAGAATCAACCGGGCAATAATGCTATTTCTAGTGTAACAACACCAATTGAAGAAGGTTCATCATCAAGTGATCAAAGTAGTACAAACTTAAGGGACTCTGGGAGGTTACCCGGGCACTATACTAATGAAGATTTGGGAGAACCAGTCATTGTCTATATCCAAGCTCATGATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

267

Amino Acids

29.79

Weight (kDa)

5.91

Isoelectric Point (pI)

67.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 112 - 155 4.2e-13 Ring finger domain
zf-RING_11 PF17123 112 - 141 7.8e-09 RING-like zinc finger
zf-rbx1 PF12678 112 - 155 1.6e-08 RING-H2 zinc finger domain
zf-C3HC4_2 PF13923 112 - 154 5.5e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015493)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24015
fragaria_vesca FvH4_5g11670
malus_domestica MD06G1111300.v1.1 MD14G1132500.v1.1
prunus_persica Prupe.5G124100_v2.0.a1
pyrus_communis pycom06g10430
rosa_chinensis RchiOBHm_Chr7g0186121
rosa_laevigata RLG00000004862
rosa_multiflora Rmu_sc0000194.1_g000030
rosa_roxburghii Rroxscaffold_3G00268410
rosa_rugosa Rorug06G0472200
rosa_samantha Rh7AG069400 Rh7BG085400 Rh7CG078700 Rh7DG078800
rosa_wichuraiana Rw7G006550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 210
AarI CACCTGC 1 cut(s) 520
Acc36I ACCTGC 1 cut(s) 520
AccBSI CCGCTC 1 cut(s) 167
AccI GTMKAC 1 cut(s) 421
AciI CCGC 1 cut(s) 165
AcsI RAATTY 1 cut(s) 356
AcvI CACGTG 1 cut(s) 403
AfaI GTAC 3 cut(s) 31, 487, 706
AflII CTTAAG 1 cut(s) 712
AflIII ACRYGT 2 cut(s) 400, 402
AgsI TTSAA 4 cut(s) 218, 268, 293, 674
AhlI ACTAGT 1 cut(s) 32
AluBI AGCT 5 cut(s) 221, 277, 289, 518, 788
AluI AGCT 5 cut(s) 221, 277, 289, 518, 788
Alw21I GWGCWC 1 cut(s) 279
Ama87I CYCGRG 1 cut(s) 734
ApeKI GCWGC 5 cut(s) 221, 433, 515, 518, 550
ApoI RAATTY 1 cut(s) 356
Asp700I GAANNNNTTC 1 cut(s) 160
AspS9I GGNCC 1 cut(s) 604
AsuC2I CCSGG 3 cut(s) 636, 735, 736
AsuII TTCGAA 1 cut(s) 360
AvaI CYCGRG 1 cut(s) 734
AvaII GGWCC 1 cut(s) 604
BaeGI GKGCMC 1 cut(s) 741
BanII GRGCYC 1 cut(s) 279
BarI GAAGNNNNNNTAC 2 cut(s) 360, 392
BbrPI CACGTG 1 cut(s) 403
BbsI GAAGAC 1 cut(s) 2
Bbv12I GWGCWC 1 cut(s) 279
BbvI GCAGC 5 cut(s) 208, 420, 505, 527, 562
BccI CCATC 3 cut(s) 122, 488, 582
BclI TGATCA 1 cut(s) 694
BcnI CCSGG 3 cut(s) 636, 735, 736
BcuI ACTAGT 1 cut(s) 32
BfaI CTAG 3 cut(s) 33, 522, 654
BfmI CTRYAG 1 cut(s) 513
BfrI CTTAAG 1 cut(s) 712
BfuAI ACCTGC 1 cut(s) 520
BisI GCNGC 5 cut(s) 222, 434, 516, 519, 551
BlsI GCNGC 5 cut(s) 223, 435, 517, 520, 552
BmcAI AGTACT 1 cut(s) 31
Bme1390I CCNGG 3 cut(s) 636, 735, 736
Bme18I GGWCC 1 cut(s) 604
BmeT110I CYCGRG 1 cut(s) 734
BmgT120I GGNCC 1 cut(s) 604
BmrFI CCNGG 3 cut(s) 636, 735, 736
BmrI ACTGGG 1 cut(s) 530
BmsI GCATC 1 cut(s) 455
BmuI ACTGGG 1 cut(s) 530
BpiI GAAGAC 1 cut(s) 2
Bpu14I TTCGAA 1 cut(s) 360
BpuEI CTTGAG 3 cut(s) 39, 263, 370
BpuMI CCSGG 3 cut(s) 636, 735, 736
BsaAI YACGTR 1 cut(s) 403
BsaBI GATNNNNATC 2 cut(s) 101, 479
BsaJI CCNNGG 2 cut(s) 197, 734
BsaWI WCCGGW 1 cut(s) 256
BsaXI ACNNNNNCTCC 2 cut(s) 753, 783
Bse1I ACTGG 5 cut(s) 188, 434, 536, 584, 767
Bse8I GATNNNNATC 2 cut(s) 101, 479
BseDI CCNNGG 2 cut(s) 197, 734
BseJI GATNNNNATC 2 cut(s) 101, 479
BseNI ACTGG 5 cut(s) 188, 434, 536, 584, 767
BseSI GKGCMC 1 cut(s) 741
BseXI GCAGC 5 cut(s) 208, 420, 505, 527, 562
BsgI GTGCAG 1 cut(s) 207
BsiHKAI GWGCWC 1 cut(s) 279
BsiHKCI CYCGRG 1 cut(s) 734
BsiSI CCGG 3 cut(s) 257, 635, 735
BslFI GGGAC 2 cut(s) 486, 731
BsmFI GGGAC 2 cut(s) 486, 731
BsoBI CYCGRG 1 cut(s) 734
Bsp119I TTCGAA 1 cut(s) 360
Bsp1286I GDGCHC 2 cut(s) 279, 741
Bsp143I GATC 1 cut(s) 694
BspACI CCGC 1 cut(s) 165
BspHI TCATGA 2 cut(s) 790, 797
BspMAI CTGCAG 1 cut(s) 517
BspMI ACCTGC 1 cut(s) 520
BspT104I TTCGAA 1 cut(s) 360
BspTI CTTAAG 1 cut(s) 712
BsrBI CCGCTC 1 cut(s) 167
BsrI ACTGG 5 cut(s) 188, 434, 536, 584, 767
BssECI CCNNGG 2 cut(s) 197, 734
BssMI GATC 1 cut(s) 694
BssNAI GTATAC 1 cut(s) 422
BssT1I CCWWGG 1 cut(s) 197
Bst1107I GTATAC 1 cut(s) 422
Bst4CI ACNGT 2 cut(s) 29, 500
Bst6I CTCTTC 2 cut(s) 137, 363
BstAFI CTTAAG 1 cut(s) 712
BstBAI YACGTR 1 cut(s) 403
BstBI TTCGAA 1 cut(s) 360
BstEII GGTNACC 1 cut(s) 729
BstKTI GATC 1 cut(s) 697
BstMBI GATC 1 cut(s) 694
BstMWI GCNNNNNNNGC 2 cut(s) 345, 459
BstPI GGTNACC 1 cut(s) 729
BstSCI CCNGG 3 cut(s) 634, 733, 734
BstSFI CTRYAG 1 cut(s) 513
BstSLI GKGCMC 1 cut(s) 741
BstV1I GCAGC 5 cut(s) 208, 420, 505, 527, 562
BstV2I GAAGAC 1 cut(s) 2
BstZ17I GTATAC 1 cut(s) 422
BtsI GCAGTG 1 cut(s) 246
BtsIMutI CAGTG 3 cut(s) 246, 427, 591
BveI ACCTGC 1 cut(s) 520
CciI TCATGA 2 cut(s) 790, 797
Cfr13I GGNCC 1 cut(s) 604
Cfr9I CCCGGG 1 cut(s) 734
Csp6I GTAC 3 cut(s) 30, 486, 705
CviAII CATG 5 cut(s) 239, 393, 410, 791, 798
CviJI RGCY 7 cut(s) 161, 221, 277, 289, 433, 518, 788
CviKI_1 RGCY 7 cut(s) 161, 221, 277, 289, 433, 518, 788
CviQI GTAC 3 cut(s) 30, 486, 705
DpnI GATC 1 cut(s) 696
DpnII GATC 1 cut(s) 694
Eam1104I CTCTTC 2 cut(s) 137, 363
EarI CTCTTC 2 cut(s) 137, 363
Ecl136II GAGCTC 1 cut(s) 277
Eco130I CCWWGG 1 cut(s) 197
Eco24I GRGCYC 1 cut(s) 279
Eco47I GGWCC 1 cut(s) 604
Eco53kI GAGCTC 1 cut(s) 277
Eco72I CACGTG 1 cut(s) 403
Eco88I CYCGRG 1 cut(s) 734
Eco91I GGTNACC 1 cut(s) 729
EcoICRI GAGCTC 1 cut(s) 277
EcoO65I GGTNACC 1 cut(s) 729
EcoRI GAATTC 1 cut(s) 356
EcoT14I CCWWGG 1 cut(s) 197
EcoT38I GRGCYC 1 cut(s) 279
ErhI CCWWGG 1 cut(s) 197
FaeI CATG 5 cut(s) 242, 396, 413, 794, 801
FalI AAGNNNNNCTT 2 cut(s) 145, 177
FaqI GGGAC 2 cut(s) 486, 731
FatI CATG 5 cut(s) 238, 392, 409, 790, 797
FbaI TGATCA 1 cut(s) 694
FblI GTMKAC 1 cut(s) 421
Fnu4HI GCNGC 5 cut(s) 222, 434, 516, 519, 551
FriOI GRGCYC 1 cut(s) 279
Fsp4HI GCNGC 5 cut(s) 222, 434, 516, 519, 551
FspBI CTAG 3 cut(s) 33, 522, 654
GluI GCNGC 5 cut(s) 222, 434, 516, 519, 551
HapII CCGG 3 cut(s) 257, 635, 735
Hin1II CATG 5 cut(s) 242, 396, 413, 794, 801
HindIII AAGCTT 1 cut(s) 287
HinfI GANTC 7 cut(s) 97, 329, 475, 580, 628, 719, 794
HpaII CCGG 3 cut(s) 257, 635, 735
Hpy166II GTNNAC 1 cut(s) 422
Hpy188I TCNGA 1 cut(s) 579
Hpy188III TCNNGA 3 cut(s) 94, 791, 798
Hpy8I GTNNAC 1 cut(s) 422
HpyAV CCTTC 4 cut(s) 94, 150, 262, 671
HpyCH4III ACNGT 2 cut(s) 29, 500
HpyCH4IV ACGT 1 cut(s) 402
HpyCH4V TGCA 6 cut(s) 224, 382, 396, 436, 462, 515
HpyF10VI GCNNNNNNNGC 2 cut(s) 345, 459
HpySE526I ACGT 1 cut(s) 402
Hsp92II CATG 5 cut(s) 242, 396, 413, 794, 801
Ksp22I TGATCA 1 cut(s) 694
Kzo9I GATC 1 cut(s) 694
LmnI GCTCC 1 cut(s) 274
Lsp1109I GCAGC 5 cut(s) 208, 420, 505, 527, 562
LweI GCATC 1 cut(s) 455
MaeI CTAG 3 cut(s) 33, 522, 654
MaeII ACGT 1 cut(s) 402
MaeIII GTNAC 2 cut(s) 658, 729
MalI GATC 1 cut(s) 696
MbiI CCGCTC 1 cut(s) 167
MboI GATC 1 cut(s) 694
MboII GAAGA 9 cut(s) 119, 124, 168, 171, 206, 323, 380, 686, 764
MfeI CAATTG 1 cut(s) 669
MhlI GDGCHC 2 cut(s) 279, 741
MluCI AATT 5 cut(s) 205, 297, 356, 374, 669
MlyI GAGTC 2 cut(s) 589, 713
MmeI TCCRAC 1 cut(s) 88
MnlI CCTC 5 cut(s) 24, 63, 158, 364, 720
MroXI GAANNNNTTC 1 cut(s) 160
MseI TTAA 2 cut(s) 69, 713
MspA1I CMGCKG 1 cut(s) 518
MspCI CTTAAG 1 cut(s) 712
MspI CCGG 3 cut(s) 257, 635, 735
MspR9I CCNGG 3 cut(s) 636, 735, 736
MunI CAATTG 1 cut(s) 669
MwoI GCNNNNNNNGC 2 cut(s) 345, 459
NciI CCSGG 3 cut(s) 636, 735, 736
NdeII GATC 1 cut(s) 694
NlaIII CATG 5 cut(s) 242, 396, 413, 794, 801
NspV TTCGAA 1 cut(s) 360
PagI TCATGA 2 cut(s) 790, 797
PaqCI CACCTGC 1 cut(s) 520
PdmI GAANNNNTTC 1 cut(s) 160
PfeI GAWTC 5 cut(s) 97, 329, 475, 628, 794
PkrI GCNGC 5 cut(s) 223, 435, 517, 520, 552
PleI GAGTC 2 cut(s) 588, 713
PmaCI CACGTG 1 cut(s) 403
PmlI CACGTG 1 cut(s) 403
PpsI GAGTC 2 cut(s) 588, 713
Ppu21I YACGTR 1 cut(s) 403
PsiI TTATAA 1 cut(s) 210
Psp124BI GAGCTC 1 cut(s) 279
PspCI CACGTG 1 cut(s) 403
PspEI GGTNACC 1 cut(s) 729
PspPI GGNCC 1 cut(s) 604
PstI CTGCAG 1 cut(s) 517
PvuII CAGCTG 1 cut(s) 518
RsaI GTAC 3 cut(s) 31, 487, 706
RsaNI GTAC 3 cut(s) 30, 486, 705
SacI GAGCTC 1 cut(s) 279
SaqAI TTAA 2 cut(s) 69, 713
SatI GCNGC 5 cut(s) 222, 434, 516, 519, 551
Sau3AI GATC 1 cut(s) 694
Sau96I GGNCC 1 cut(s) 604
ScaI AGTACT 1 cut(s) 31
SchI GAGTC 2 cut(s) 589, 713
ScrFI CCNGG 3 cut(s) 636, 735, 736
SduI GDGCHC 2 cut(s) 279, 741
SfaNI GCATC 1 cut(s) 455
SfcI CTRYAG 1 cut(s) 513
SfuI TTCGAA 1 cut(s) 360
SinI GGWCC 1 cut(s) 604
SmaI CCCGGG 1 cut(s) 736
SmlI CTYRAG 4 cut(s) 54, 278, 349, 712
SmoI CTYRAG 4 cut(s) 54, 278, 349, 712
SpeI ACTAGT 1 cut(s) 32
Sse9I AATT 5 cut(s) 205, 297, 356, 374, 669
SsiI CCGC 1 cut(s) 165
SspMI CTAG 3 cut(s) 33, 522, 654
SstI GAGCTC 1 cut(s) 279
StyD4I CCNGG 3 cut(s) 634, 733, 734
StyI CCWWGG 1 cut(s) 197
TaaI ACNGT 2 cut(s) 29, 500
TaiI ACGT 1 cut(s) 405
TaqI TCGA 4 cut(s) 95, 360, 495, 531
TasI AATT 5 cut(s) 205, 297, 356, 374, 669
TatI WGTACW 2 cut(s) 29, 704
TfiI GAWTC 5 cut(s) 97, 329, 475, 628, 794
Tru1I TTAA 2 cut(s) 69, 713
Tru9I TTAA 2 cut(s) 69, 713
TscAI CASTG 3 cut(s) 253, 434, 591
TseI GCWGC 5 cut(s) 221, 433, 515, 518, 550
TspDTI ATGAA 6 cut(s) 76, 324, 398, 672, 765, 786
TspMI CCCGGG 1 cut(s) 734
TspRI CASTG 3 cut(s) 253, 434, 591
Vha464I CTTAAG 1 cut(s) 712
VpaK11BI GGWCC 1 cut(s) 604
XapI RAATTY 1 cut(s) 356
XmaI CCCGGG 1 cut(s) 734
XmiI GTMKAC 1 cut(s) 421
XmnI GAANNNNTTC 1 cut(s) 160
XspI CTAG 3 cut(s) 33, 522, 654
ZrmI AGTACT 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.