MD06G1135800.v1.1

Mitochondrial import inner membrane translocase subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0015070
Physical Location & Seq
Forward (+)
28078307 .. 28080056
1750 bp
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UTR
Exon/CDS
Intron
MD06G1135800.v1.1.491

Sequence Viewer

Length: 237 bp
ATGGATCCTTCAGCAATGAACAATCCTGAACTGCTTAACTTCATAAACCAAGAGAAAGAAAGAGCAATGGTGAATGAGATGGTGGGAAAGCTTACGAATGTGTGTTGGGACAAGTGCATCACTGGTACCCCCGGGAGCAAGTTCAGTTCCAGCGAGTCCGCTTGCCTGGCAAACTGCGCCCGCCGATATCTTGATATGAGTATGATTATTATGAAGCGTTTTCAGAGCATGCAATGA

Protein Analysis

79

Amino Acids

8.84

Weight (kDa)

7.64

Isoelectric Point (pI)

46.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-Tim10_DDP PF02953 13 - 76 4.8e-26 Tim10/DDP family zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015070)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50810
fragaria_vesca FvH4_5g08990
malus_domestica MD06G1135800.v1.1 MD14G1151000.v1.1
prunus_persica Prupe.5G146200_v2.0.a1
pyrus_communis pycom06g12700 pycom14g12540
rosa_chinensis RchiOBHm_Chr7g0189291
rosa_multiflora Rmu_sc0004318.1_g000016
rosa_roxburghii Rroxscaffold_3G00265550
rosa_rugosa Rorug06G0496900
rosa_samantha Rh7AG102000 Rh7BG104600 Rh7CG105900 Rh7DG103700
rosa_wichuraiana Rw7G008810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 125
AccB1I GGYRCC 1 cut(s) 125
AciI CCGC 2 cut(s) 159, 181
AclWI GGATC 1 cut(s) 12
AfaI GTAC 1 cut(s) 127
AjnI CCWGG 1 cut(s) 165
AluBI AGCT 1 cut(s) 91
AluI AGCT 1 cut(s) 91
AlwI GGATC 1 cut(s) 12
Ama87I CYCGRG 1 cut(s) 131
Asp718I GGTACC 1 cut(s) 125
AspLEI GCGC 1 cut(s) 179
AsuC2I CCSGG 2 cut(s) 132, 133
AsuHPI GGTGA 1 cut(s) 82
AvaI CYCGRG 1 cut(s) 131
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 125
BccI CCATC 1 cut(s) 73
BciT130I CCWGG 1 cut(s) 167
BcnI CCSGG 2 cut(s) 132, 133
Bme1390I CCNGG 3 cut(s) 132, 133, 167
BmeT110I CYCGRG 1 cut(s) 131
BmiI GGNNCC 2 cut(s) 6, 127
BmrFI CCNGG 3 cut(s) 132, 133, 167
BmsI GCATC 1 cut(s) 126
BpuMI CCSGG 2 cut(s) 132, 133
BsaJI CCNNGG 2 cut(s) 130, 131
Bse1I ACTGG 1 cut(s) 127
Bse3DI GCAATG 2 cut(s) 21, 72
BseBI CCWGG 1 cut(s) 167
BseDI CCNNGG 2 cut(s) 130, 131
BseMI GCAATG 2 cut(s) 21, 72
BseNI ACTGG 1 cut(s) 127
BshNI GGYRCC 1 cut(s) 125
BsiHKCI CYCGRG 1 cut(s) 131
BsiSI CCGG 1 cut(s) 132
BslFI GGGAC 1 cut(s) 122
BsmFI GGGAC 1 cut(s) 122
BsoBI CYCGRG 1 cut(s) 131
Bsp143I GATC 1 cut(s) 4
BspACI CCGC 2 cut(s) 159, 181
BspLI GGNNCC 2 cut(s) 6, 127
BspPI GGATC 1 cut(s) 12
BspT107I GGYRCC 1 cut(s) 125
BsrDI GCAATG 2 cut(s) 21, 72
BsrI ACTGG 1 cut(s) 127
BssECI CCNNGG 2 cut(s) 130, 131
BssMI GATC 1 cut(s) 4
Bst2UI CCWGG 1 cut(s) 167
BstC8I GCNNGC 3 cut(s) 163, 181, 230
BstHHI GCGC 1 cut(s) 179
BstKTI GATC 1 cut(s) 7
BstMBI GATC 1 cut(s) 4
BstMWI GCNNNNNNNGC 2 cut(s) 167, 176
BstNI CCWGG 1 cut(s) 167
BstNSI RCATGY 1 cut(s) 232
BstSCI CCNGG 3 cut(s) 130, 131, 165
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BtsIMutI CAGTG 1 cut(s) 120
Cac8I GCNNGC 3 cut(s) 163, 181, 230
CfoI GCGC 1 cut(s) 179
Cfr9I CCCGGG 1 cut(s) 131
Csp6I GTAC 1 cut(s) 126
CviAII CATG 1 cut(s) 229
CviJI RGCY 1 cut(s) 91
CviKI_1 RGCY 1 cut(s) 91
CviQI GTAC 1 cut(s) 126
DpnI GATC 1 cut(s) 6
DpnII GATC 1 cut(s) 4
Eco32I GATATC 1 cut(s) 188
Eco88I CYCGRG 1 cut(s) 131
EcoRII CCWGG 1 cut(s) 165
EcoRV GATATC 1 cut(s) 188
FaeI CATG 1 cut(s) 232
FaiI YATR 5 cut(s) 44, 197, 203, 212, 230
FaqI GGGAC 1 cut(s) 122
FatI CATG 1 cut(s) 228
FauI CCCGC 1 cut(s) 188
GlaI GCGC 1 cut(s) 178
HapII CCGG 1 cut(s) 132
HhaI GCGC 1 cut(s) 179
Hin1II CATG 1 cut(s) 232
Hin6I GCGC 1 cut(s) 177
HinP1I GCGC 1 cut(s) 177
HindIII AAGCTT 1 cut(s) 89
HinfI GANTC 1 cut(s) 155
HpaII CCGG 1 cut(s) 132
HphI GGTGA 1 cut(s) 82
Hpy188I TCNGA 1 cut(s) 225
Hpy188III TCNNGA 2 cut(s) 26, 191
HpyAV CCTTC 1 cut(s) 18
HpyCH4V TGCA 2 cut(s) 117, 232
HpyF10VI GCNNNNNNNGC 2 cut(s) 167, 176
Hsp92II CATG 1 cut(s) 232
HspAI GCGC 1 cut(s) 177
KpnI GGTACC 1 cut(s) 129
Kzo9I GATC 1 cut(s) 4
LmnI GCTCC 1 cut(s) 135
LpnPI CCDG 6 cut(s) 39, 108, 145, 152, 163, 179
LweI GCATC 1 cut(s) 126
MalI GATC 1 cut(s) 6
MboI GATC 1 cut(s) 4
MflI RGATCY 1 cut(s) 4
MlyI GAGTC 1 cut(s) 164
MseI TTAA 1 cut(s) 36
MspI CCGG 1 cut(s) 132
MspR9I CCNGG 3 cut(s) 132, 133, 167
MvaI CCWGG 1 cut(s) 167
MwoI GCNNNNNNNGC 2 cut(s) 167, 176
NciI CCSGG 2 cut(s) 132, 133
NdeII GATC 1 cut(s) 4
NlaIII CATG 1 cut(s) 232
NlaIV GGNNCC 2 cut(s) 6, 127
NspI RCATGY 1 cut(s) 232
PaeI GCATGC 1 cut(s) 232
PleI GAGTC 1 cut(s) 163
PpsI GAGTC 1 cut(s) 163
Psp6I CCWGG 1 cut(s) 165
PspGI CCWGG 1 cut(s) 165
PspN4I GGNNCC 2 cut(s) 6, 127
PsuI RGATCY 1 cut(s) 4
RsaI GTAC 1 cut(s) 127
RsaNI GTAC 1 cut(s) 126
SaqAI TTAA 1 cut(s) 36
Sau3AI GATC 1 cut(s) 4
SchI GAGTC 1 cut(s) 164
ScrFI CCNGG 3 cut(s) 132, 133, 167
SetI ASST 1 cut(s) 93
SfaNI GCATC 1 cut(s) 126
SmaI CCCGGG 1 cut(s) 133
SphI GCATGC 1 cut(s) 232
SsiI CCGC 2 cut(s) 159, 181
StyD4I CCNGG 3 cut(s) 130, 131, 165
Tru1I TTAA 1 cut(s) 36
Tru9I TTAA 1 cut(s) 36
TscAI CASTG 1 cut(s) 127
TspDTI ATGAA 3 cut(s) 31, 32, 227
TspMI CCCGGG 1 cut(s) 131
TspRI CASTG 1 cut(s) 127
XceI RCATGY 1 cut(s) 232
XmaI CCCGGG 1 cut(s) 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.