Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0013669

Physical Location & Seq

Genomic Coordinates Reverse (-)

28675935 .. 28679134

Gene Structure 3200 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1143000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1629 bp

ATGAATAATAATAATGATCAGTCCAAGAATGTGGGCGTGCCACCTCACTTTGGCAATTCTGGGCCGGTGCCGCAACCTATGGCCATGAATCACCAGCCTAATCTTCTCACTCAATCGCAGCCTCCGACACAGGGGGGAGCTCATTTTCCGGGACACTTCCAGTTGTCTGAGCCCCAAGCGCAGGCACTTGCACAGGCGCAGTATGTGAATGCTCACGCACAAGGCCAAACTCAAGCTGCCCATGCCCAATTTGTTCAGTTGCAAGCTCAAGCTCAGTCACGTGCCCAGTTGCATAATGCGAATACTAGTAATGCTGGTGCTTCATCACCCTCTTTGGCAACGCCTAGCACAGGAACTGCTAAGCGGGTGAACCAGAGGCCGCCTTCTAGACCTCCAGGTTCATCTAGTGCCAACACGGGTTCACCATTTAAAACCATGGAGCTCACCCCAGCTGCTCGTAGAAAGAAGCGAAAGCTACCTGATAAACAAATACCGGACAAAGTGGCTGCACTTCTGCCAGAGTCCGGTCTTTATACTCAGTTGCTTGAATTTGAAGCTCGTGTTGATGCTGCTTTGGCAAGGAAGAAGATGGACATCCAGGAGTCCCTCAAATGTCCTCCCCGTGTTCAAAAAACACTTAGAGTATATGTATTCAACACCTTTGCGAATCAGACACAAACAAGTCCTGAGAAGACAAATGCAGAACCCCCTTCATGGTCACTTAAGATAATTGGGAGGTTACTGGAAGACGGGCGGGATCCTGTGGTTGCTGGATTGACACCGAAATCAAGGGCAAAATTTTCATCCCTGTTCAAGAAAGTTACCATATACTTGGACCAAACCCTCTATCCAGATAACCAAGTGATTTTATGGGAAAGTTCCCGTTCACCCTCTCTTCATGACGGCTTTGAGGTGAAAAGAAAAGGGGATAAGGAGTTTACTGCAATACTAAGACTAGAAATGAATTATGTACCTGAGAAATTCAAACTTTCACCGGCCCTCCATGAAGTTCTGGGAATTGAGGTAGAGACCCGACCCAGAATTATAGCTGCTATTTGGCACTATGTCAAGGCCAGGAAGTTGCAGAACCCAAATGACCCTACGTTCTTTGTGTGTGATCCACCACTTCAGAAGATTTTTGGGGAAGAGAAGATGAAGTTTTCAATGGTTTCACAAAAGATATCACAGCATTTAACGCCACCACAGCCTATACATATAGAGCATAAGATAAAACTTTCAGGAAATTGTCCAGCTGGAACTACTTGCTATGATATACTGGTTGATGTACCCATGATATTAGAGAAGGAGATGTCTGCGTTCTTGGGAAGCACCGAGAGGAGCAAAGAGATTGATGCTTGTGATGAATTGATATGTGCATCCATAAAAAAAATCCATGAACATCGACGGCGGCGAGCTTTCTTTCTTGGGTTCAGTCAATCTCCTGCAGAGTTTATTAATTCTTTGATTGCTTCCCAGAGCAAGGATCTAAAGCTTGTTGCTGGAGACGCTAGTCGTAATGTGGAAAAGGAGCGCCGCACGGATTTCTATAATCAACCCTGGGTTGAGGATGCTGTTATTCGTTACCTGAACCGAAAGTCAGCCGGAAGTGATGCTCCCGGAAGCACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0008152 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009909 GO:0009987 GO:0010224 GO:0010468 GO:0019222 GO:0022622 GO:0032501 GO:0032502 GO:0033554 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0048364 GO:0048580 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050896 GO:0051239 GO:0051716 GO:0060255 GO:0065007 GO:0071704 GO:0090304 GO:0099402 GO:1901360 GO:2000024 GO:2000026 GO:2000241

KEGG Pathways

Metabolic & Signaling

ko04714 ko05225 map04714 map05225

Pfam Domains

Protein Families

SWIB

Protein Analysis

543

Amino Acids

60.23

Weight (kDa)

9.48

Isoelectric Point (pI)

56.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SWIB	PF02201	326 - 398	1.5e-24	SWIB/MDM2 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013669)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g08160 FvH4_5g08160 FvH4_5g08160
malus_domestica	MD06G1143000.v1.1 MD14G1158200.v1.1
prunus_persica	Prupe.5G152300_v2.0.a1 Prupe.5G152300_v2.0.a1
pyrus_communis	pycom06g13280 pycom14g13180
rosa_chinensis	RchiOBHm_Chr7g0190351
rosa_laevigata	RLG00000004539
rosa_multiflora	Rmu_ssc0000038.1_g000003
rosa_roxburghii	Rroxscaffold_3G00264700
rosa_rugosa	Rorug06G0504000
rosa_samantha	Rh7AG109800 Rh7BG112400 Rh7DG112800
rosa_wichuraiana	Rw7G009530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	67
AciI	CCGC	6 cut(s)	71, 364, 380, 754, 1408, 1534
AclWI	GGATC	4 cut(s)	752, 765, 1112, 1491
AcoI	YGGCCR	1 cut(s)	81
AcsI	RAATTY	3 cut(s)	548, 797, 980
AcuI	CTGAAG	1 cut(s)	1112
AcvI	CACGTG	1 cut(s)	281
AfaI	GTAC	2 cut(s)	972, 1287
AfiI	CCNNNNNNNGG	7 cut(s)	50, 131, 181, 350, 524, 714, 1480
AflII	CTTAAG	1 cut(s)	722
AgsI	TTSAA	7 cut(s)	548, 554, 629, 655, 814, 985, 1164
AhlI	ACTAGT	1 cut(s)	305
AjnI	CCWGG	4 cut(s)	394, 597, 1073, 1556
Alw21I	GWGCWC	2 cut(s)	142, 444
Alw26I	GTCTC	2 cut(s)	1022, 1497
AlwI	GGATC	4 cut(s)	752, 765, 1112, 1491
AlwNI	CAGNNNCTG	1 cut(s)	356
AoxI	GGCC	6 cut(s)	62, 81, 223, 377, 996, 1071
ApeKI	GCWGC	6 cut(s)	118, 236, 452, 506, 569, 1049
ApoI	RAATTY	3 cut(s)	548, 797, 980
AseI	ATTAAT	1 cut(s)	1455
AspLEI	GCGC	3 cut(s)	181, 199, 1533
AspS9I	GGNCC	3 cut(s)	62, 835, 997
AsuC2I	CCSGG	2 cut(s)	150, 1617
AsuHPI	GGTGA	8 cut(s)	83, 318, 379, 414, 436, 879, 925, 984
AvaII	GGWCC	1 cut(s)	835
BaeGI	GKGCMC	1 cut(s)	286
BalI	TGGCCA	1 cut(s)	83
BamHI	GGATCC	1 cut(s)	757
BanI	GGYRCC	1 cut(s)	67
BanII	GRGCYC	3 cut(s)	142, 174, 444
BauI	CACGAG	1 cut(s)	558
BbrPI	CACGTG	1 cut(s)	281
BbsI	GAAGAC	2 cut(s)	698, 753
Bbv12I	GWGCWC	2 cut(s)	142, 444
BbvI	GCAGC	6 cut(s)	130, 223, 439, 493, 556, 1036
BccI	CCATC	1 cut(s)	583
BceAI	ACGGC	2 cut(s)	919, 1421
BciT130I	CCWGG	4 cut(s)	396, 599, 1075, 1558
BclI	TGATCA	1 cut(s)	16
BcnI	CCSGG	2 cut(s)	150, 1617
BcoDI	GTCTC	2 cut(s)	1022, 1497
BcuI	ACTAGT	1 cut(s)	305
BfaI	CTAG	6 cut(s)	306, 345, 387, 405, 956, 1509
BfmI	CTRYAG	1 cut(s)	1443
BfoI	RGCGCY	1 cut(s)	1534
BfrI	CTTAAG	1 cut(s)	722
BlpI	GCTNAGC	1 cut(s)	360
Bme1390I	CCNGG	6 cut(s)	150, 396, 599, 1075, 1558, 1617
Bme18I	GGWCC	1 cut(s)	835
BmgT120I	GGNCC	3 cut(s)	62, 835, 997
BmiI	GGNNCC	2 cut(s)	69, 759
BmrFI	CCNGG	6 cut(s)	150, 396, 599, 1075, 1558, 1617
BmrI	ACTGGG	1 cut(s)	280
BmsI	GCATC	5 cut(s)	556, 1342, 1385, 1558, 1600
BmuI	ACTGGG	1 cut(s)	280
BoxI	GACNNNNGTC	1 cut(s)	1509
BpiI	GAAGAC	2 cut(s)	698, 753
BpmI	CTGGAG	2 cut(s)	378, 1521
Bpu1102I	GCTNAGC	1 cut(s)	360
BpuEI	CTTGAG	2 cut(s)	216, 252
BpuMI	CCSGG	2 cut(s)	150, 1617
BsaAI	YACGTR	1 cut(s)	281
BsaBI	GATNNNNATC	1 cut(s)	593
BsaI	GGTCTC	1 cut(s)	1022
BsaJI	CCNNGG	3 cut(s)	435, 1556, 1557
BsaWI	WCCGGW	2 cut(s)	493, 524
BsaXI	ACNNNNNCTCC	2 cut(s)	984, 1014
Bsc4I	CCNNNNNNNGG	7 cut(s)	50, 131, 181, 350, 524, 714, 1480
Bse118I	RCCGGY	2 cut(s)	64, 994
Bse1I	ACTGG	4 cut(s)	160, 286, 747, 1281
Bse8I	GATNNNNATC	1 cut(s)	593
BseBI	CCWGG	4 cut(s)	396, 599, 1075, 1558
BseDI	CCNNGG	3 cut(s)	435, 1556, 1557
BseGI	GGATG	4 cut(s)	594, 803, 1376, 1573
BseJI	GATNNNNATC	1 cut(s)	593
BseLI	CCNNNNNNNGG	7 cut(s)	50, 131, 181, 350, 524, 714, 1480
BseMII	CTCAG	5 cut(s)	159, 287, 551, 678, 966
BseNI	ACTGG	4 cut(s)	160, 286, 747, 1281
BseRI	GAGGAG	1 cut(s)	1351
BseSI	GKGCMC	1 cut(s)	286
BseXI	GCAGC	6 cut(s)	130, 223, 439, 493, 556, 1036
BseYI	CCCAGC	1 cut(s)	448
BsgI	GTGCAG	1 cut(s)	492
BshFI	GGCC	6 cut(s)	64, 83, 225, 379, 998, 1073
BshNI	GGYRCC	1 cut(s)	67
BsiHKAI	GWGCWC	2 cut(s)	142, 444
BsiSI	CCGG	7 cut(s)	65, 149, 494, 525, 995, 1602, 1617
BslFI	GGGAC	2 cut(s)	165, 589
BslI	CCNNNNNNNGG	7 cut(s)	50, 131, 181, 350, 524, 714, 1480
BsmAI	GTCTC	2 cut(s)	1022, 1497
BsmBI	CGTCTC	1 cut(s)	1497
BsmFI	GGGAC	2 cut(s)	165, 589
BsmI	GAATGC	1 cut(s)	214
BsnI	GGCC	6 cut(s)	64, 83, 225, 379, 998, 1073
Bso31I	GGTCTC	1 cut(s)	1022
Bsp1286I	GDGCHC	4 cut(s)	142, 174, 286, 444
Bsp143I	GATC	4 cut(s)	16, 757, 1117, 1483
Bsp1720I	GCTNAGC	1 cut(s)	360
Bsp19I	CCATGG	1 cut(s)	435
BspACI	CCGC	6 cut(s)	71, 364, 380, 754, 1408, 1534
BspANI	GGCC	6 cut(s)	64, 83, 225, 379, 998, 1073
BspCNI	CTCAG	5 cut(s)	160, 286, 550, 679, 967
BspHI	TCATGA	1 cut(s)	898
BspLI	GGNNCC	2 cut(s)	69, 759
BspMAI	CTGCAG	1 cut(s)	1447
BspPI	GGATC	4 cut(s)	752, 765, 1112, 1491
BspT107I	GGYRCC	1 cut(s)	67
BspTI	CTTAAG	1 cut(s)	722
BspTNI	GGTCTC	1 cut(s)	1022
BsrFI	RCCGGY	2 cut(s)	64, 994
BsrI	ACTGG	4 cut(s)	160, 286, 747, 1281
BssAI	RCCGGY	2 cut(s)	64, 994
BssECI	CCNNGG	3 cut(s)	435, 1556, 1557
BssMI	GATC	4 cut(s)	16, 757, 1117, 1483
BssSI	CACGAG	1 cut(s)	558
BssT1I	CCWWGG	1 cut(s)	435
Bst2BI	CACGAG	1 cut(s)	558
Bst2UI	CCWGG	4 cut(s)	396, 599, 1075, 1558
Bst6I	CTCTTC	2 cut(s)	900, 1140
BstAFI	CTTAAG	1 cut(s)	722
BstBAI	YACGTR	1 cut(s)	281
BstC8I	GCNNGC	4 cut(s)	38, 183, 264, 1413
BstDEI	CTNAG	8 cut(s)	168, 273, 360, 537, 638, 687, 950, 975
BstDSI	CCRYGG	1 cut(s)	435
BstENI	CCTNNNNNAGG	1 cut(s)	348
BstF5I	GGATG	4 cut(s)	594, 803, 1376, 1573
BstH2I	RGCGCY	1 cut(s)	1534
BstHHI	GCGC	3 cut(s)	181, 199, 1533
BstKTI	GATC	4 cut(s)	19, 760, 1120, 1486
BstMAI	GTCTC	2 cut(s)	1022, 1497
BstMBI	GATC	4 cut(s)	16, 757, 1117, 1483
BstMWI	GCNNNNNNNGC	7 cut(s)	70, 178, 242, 575, 1195, 1204, 1505
BstNI	CCWGG	4 cut(s)	396, 599, 1075, 1558
BstPAI	GACNNNNGTC	1 cut(s)	1509
BstSCI	CCNGG	6 cut(s)	148, 394, 597, 1073, 1556, 1615
BstSFI	CTRYAG	1 cut(s)	1443
BstSLI	GKGCMC	1 cut(s)	286
BstV1I	GCAGC	6 cut(s)	130, 223, 439, 493, 556, 1036
BstV2I	GAAGAC	2 cut(s)	698, 753
BstX2I	RGATCY	2 cut(s)	757, 1483
BstXI	CCANNNNNNTGG	2 cut(s)	31, 832
BstYI	RGATCY	2 cut(s)	757, 1483
BsuRI	GGCC	6 cut(s)	64, 83, 225, 379, 998, 1073
BtgI	CCRYGG	1 cut(s)	435
BtsCI	GGATG	4 cut(s)	594, 803, 1376, 1573
Cac8I	GCNNGC	4 cut(s)	38, 183, 264, 1413
CaiI	CAGNNNCTG	1 cut(s)	356
CciI	TCATGA	1 cut(s)	898
CfoI	GCGC	3 cut(s)	181, 199, 1533
Cfr10I	RCCGGY	2 cut(s)	64, 994
Cfr13I	GGNCC	3 cut(s)	62, 835, 997
CseI	GACGC	1 cut(s)	1514
Csp6I	GTAC	2 cut(s)	971, 1286
CviAII	CATG	8 cut(s)	85, 242, 436, 714, 899, 1004, 1291, 1394
CviQI	GTAC	2 cut(s)	971, 1286
DdeI	CTNAG	8 cut(s)	168, 273, 360, 537, 638, 687, 950, 975
DpnI	GATC	4 cut(s)	18, 759, 1119, 1485
DpnII	GATC	4 cut(s)	16, 757, 1117, 1483
DraI	TTTAAA	1 cut(s)	430
EaeI	YGGCCR	1 cut(s)	81
Eam1104I	CTCTTC	2 cut(s)	900, 1140
EarI	CTCTTC	2 cut(s)	900, 1140
Ecl136II	GAGCTC	2 cut(s)	140, 442
Eco130I	CCWWGG	1 cut(s)	435
Eco24I	GRGCYC	3 cut(s)	142, 174, 444
Eco31I	GGTCTC	1 cut(s)	1022
Eco32I	GATATC	1 cut(s)	1182
Eco47I	GGWCC	1 cut(s)	835
Eco53kI	GAGCTC	2 cut(s)	140, 442
Eco57I	CTGAAG	1 cut(s)	1112
Eco72I	CACGTG	1 cut(s)	281
EcoICRI	GAGCTC	2 cut(s)	140, 442
EcoNI	CCTNNNNNAGG	1 cut(s)	348
EcoRII	CCWGG	4 cut(s)	394, 597, 1073, 1556
EcoRV	GATATC	1 cut(s)	1182
EcoT14I	CCWWGG	1 cut(s)	435
EcoT38I	GRGCYC	3 cut(s)	142, 174, 444
ErhI	CCWWGG	1 cut(s)	435
Esp3I	CGTCTC	1 cut(s)	1497
FaeI	CATG	8 cut(s)	88, 245, 439, 717, 902, 1007, 1294, 1397
FaqI	GGGAC	2 cut(s)	165, 589
FatI	CATG	8 cut(s)	84, 241, 435, 713, 898, 1003, 1290, 1393
FauI	CCCGC	2 cut(s)	357, 747
FbaI	TGATCA	1 cut(s)	16
FokI	GGATG	4 cut(s)	581, 790, 1363, 1580
FriOI	GRGCYC	3 cut(s)	142, 174, 444
FspBI	CTAG	6 cut(s)	306, 345, 387, 405, 956, 1509
GlaI	GCGC	3 cut(s)	180, 198, 1532
GsaI	CCCAGC	1 cut(s)	452
GsuI	CTGGAG	2 cut(s)	378, 1521
HaeII	RGCGCY	1 cut(s)	1534
HaeIII	GGCC	6 cut(s)	64, 83, 225, 379, 998, 1073
HapII	CCGG	7 cut(s)	65, 149, 494, 525, 995, 1602, 1617
HgaI	GACGC	1 cut(s)	1514
HhaI	GCGC	3 cut(s)	181, 199, 1533
Hin1II	CATG	8 cut(s)	88, 245, 439, 717, 902, 1007, 1294, 1397
Hin6I	GCGC	3 cut(s)	179, 197, 1531
HinP1I	GCGC	3 cut(s)	179, 197, 1531
HindIII	AAGCTT	1 cut(s)	1490
HinfI	GANTC	4 cut(s)	88, 521, 602, 667
HpaII	CCGG	7 cut(s)	65, 149, 494, 525, 995, 1602, 1617
HphI	GGTGA	8 cut(s)	83, 318, 379, 414, 436, 879, 925, 984
Hpy166II	GTNNAC	4 cut(s)	370, 422, 887, 939
Hpy188I	TCNGA	4 cut(s)	126, 169, 672, 1131
Hpy188III	TCNNGA	6 cut(s)	387, 686, 814, 851, 899, 1239
Hpy8I	GTNNAC	4 cut(s)	370, 422, 887, 939
Hpy99I	CGWCG	1 cut(s)	1407
HpyAV	CCTTC	3 cut(s)	393, 720, 1297
HpyCH4IV	ACGT	2 cut(s)	280, 1103
HpyCH4V	TGCA	9 cut(s)	191, 262, 292, 509, 701, 944, 1084, 1376, 1445
HpyF10VI	GCNNNNNNNGC	7 cut(s)	70, 178, 242, 575, 1195, 1204, 1505
HpyF3I	CTNAG	8 cut(s)	168, 273, 360, 537, 638, 687, 950, 975
HpySE526I	ACGT	2 cut(s)	280, 1103
Hsp92II	CATG	8 cut(s)	88, 245, 439, 717, 902, 1007, 1294, 1397
HspAI	GCGC	3 cut(s)	179, 197, 1531
Ksp22I	TGATCA	1 cut(s)	16
Kzo9I	GATC	4 cut(s)	16, 757, 1117, 1483
LmnI	GCTCC	5 cut(s)	137, 439, 1338, 1528, 1618
Lsp1109I	GCAGC	6 cut(s)	130, 223, 439, 493, 556, 1036
LweI	GCATC	5 cut(s)	556, 1342, 1385, 1558, 1600
MaeI	CTAG	6 cut(s)	306, 345, 387, 405, 956, 1509
MaeII	ACGT	2 cut(s)	280, 1103
MaeIII	GTNAC	5 cut(s)	276, 717, 738, 820, 1580
MalI	GATC	4 cut(s)	18, 759, 1119, 1485
MboI	GATC	4 cut(s)	16, 757, 1117, 1483
MboII	GAAGA	9 cut(s)	95, 595, 598, 703, 758, 887, 1144, 1157, 1162
MflI	RGATCY	2 cut(s)	757, 1483
MhlI	GDGCHC	4 cut(s)	142, 174, 286, 444
MlsI	TGGCCA	1 cut(s)	83
MluNI	TGGCCA	1 cut(s)	83
MlyI	GAGTC	2 cut(s)	530, 611
MmeI	TCCRAC	1 cut(s)	149
Mox20I	TGGCCA	1 cut(s)	83
MscI	TGGCCA	1 cut(s)	83
MseI	TTAA	4 cut(s)	429, 723, 1193, 1455
Msp20I	TGGCCA	1 cut(s)	83
MspA1I	CMGCKG	2 cut(s)	452, 1253
MspCI	CTTAAG	1 cut(s)	722
MspI	CCGG	7 cut(s)	65, 149, 494, 525, 995, 1602, 1617
MspR9I	CCNGG	6 cut(s)	150, 396, 599, 1075, 1558, 1617
Mva1269I	GAATGC	1 cut(s)	214
MvaI	CCWGG	4 cut(s)	396, 599, 1075, 1558
MwoI	GCNNNNNNNGC	7 cut(s)	70, 178, 242, 575, 1195, 1204, 1505
NciI	CCSGG	2 cut(s)	150, 1617
NcoI	CCATGG	1 cut(s)	435
NdeII	GATC	4 cut(s)	16, 757, 1117, 1483
NlaIII	CATG	8 cut(s)	88, 245, 439, 717, 902, 1007, 1294, 1397
NlaIV	GGNNCC	2 cut(s)	69, 759
NmuCI	GTSAC	2 cut(s)	276, 717
PagI	TCATGA	1 cut(s)	898
PasI	CCCWGGG	1 cut(s)	1557
PcsI	WCGNNNNNNNCGW	2 cut(s)	122, 1408
PctI	GAATGC	1 cut(s)	214
PfeI	GAWTC	2 cut(s)	88, 667
PfoI	TCCNGGA	3 cut(s)	148, 597, 1615
PleI	GAGTC	2 cut(s)	529, 610
PmaCI	CACGTG	1 cut(s)	281
PmlI	CACGTG	1 cut(s)	281
PpsI	GAGTC	2 cut(s)	529, 610
Ppu21I	YACGTR	1 cut(s)	281
PshAI	GACNNNNGTC	1 cut(s)	1509
PshBI	ATTAAT	1 cut(s)	1455
Psp124BI	GAGCTC	2 cut(s)	142, 444
Psp6I	CCWGG	4 cut(s)	394, 597, 1073, 1556
PspCI	CACGTG	1 cut(s)	281
PspFI	CCCAGC	1 cut(s)	448
PspGI	CCWGG	4 cut(s)	394, 597, 1073, 1556
PspN4I	GGNNCC	2 cut(s)	69, 759
PspPI	GGNCC	3 cut(s)	62, 835, 997
PstI	CTGCAG	1 cut(s)	1447
PstNI	CAGNNNCTG	1 cut(s)	356
PsuI	RGATCY	2 cut(s)	757, 1483
PvuII	CAGCTG	2 cut(s)	452, 1253
RsaI	GTAC	2 cut(s)	972, 1287
RsaNI	GTAC	2 cut(s)	971, 1286
SacI	GAGCTC	2 cut(s)	142, 444
SaqAI	TTAA	4 cut(s)	429, 723, 1193, 1455
Sau3AI	GATC	4 cut(s)	16, 757, 1117, 1483
Sau96I	GGNCC	3 cut(s)	62, 835, 997
SchI	GAGTC	2 cut(s)	530, 611
ScrFI	CCNGG	6 cut(s)	150, 396, 599, 1075, 1558, 1617
SduI	GDGCHC	4 cut(s)	142, 174, 286, 444
SfaNI	GCATC	5 cut(s)	556, 1342, 1385, 1558, 1600
SfcI	CTRYAG	1 cut(s)	1443
SinI	GGWCC	1 cut(s)	835
SmlI	CTYRAG	3 cut(s)	231, 267, 722
SmoI	CTYRAG	3 cut(s)	231, 267, 722
SpeI	ACTAGT	1 cut(s)	305
SsiI	CCGC	6 cut(s)	71, 364, 380, 754, 1408, 1534
SspMI	CTAG	6 cut(s)	306, 345, 387, 405, 956, 1509
SstI	GAGCTC	2 cut(s)	142, 444
StyD4I	CCNGG	6 cut(s)	148, 394, 597, 1073, 1556, 1615
StyI	CCWWGG	1 cut(s)	435
TaiI	ACGT	2 cut(s)	283, 1106
TaqI	TCGA	1 cut(s)	1402
TauI	GCSGC	4 cut(s)	73, 382, 1411, 1536
TfiI	GAWTC	2 cut(s)	88, 667
Tru1I	TTAA	4 cut(s)	429, 723, 1193, 1455
Tru9I	TTAA	4 cut(s)	429, 723, 1193, 1455
TseFI	GTSAC	2 cut(s)	276, 717
TseI	GCWGC	6 cut(s)	118, 236, 452, 506, 569, 1049
Tsp45I	GTSAC	2 cut(s)	276, 717
TspGWI	ACGGA	1 cut(s)	1553
Vha464I	CTTAAG	1 cut(s)	722
VpaK11BI	GGWCC	1 cut(s)	835
VspI	ATTAAT	1 cut(s)	1455
XagI	CCTNNNNNAGG	1 cut(s)	348
XapI	RAATTY	3 cut(s)	548, 797, 980
XbaI	TCTAGA	1 cut(s)	386
XspI	CTAG	6 cut(s)	306, 345, 387, 405, 956, 1509

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.