Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0013669

Physical Location & Seq

Genomic Coordinates Reverse (-)

25228011 .. 25230990

Gene Structure 2980 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1158200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1626 bp

ATGAATAATAATAATCAGCCCAGGAATGTGGGGGTGCCACCCCACTTTGGCAATTCTGGGGCGGTGTCGCAACCTATGACCATGAATCACCAGCCTCATCTTCTCACTCAATCGCAGCCTCCGACACAAGGGGGAGCTCATTTTCCAGGGCACTTTCAGTTGTCTGAGCCGCAAGCGCAGGCACTTGCACAGGCACAGTATGTGAATGCTCACGCACAAGCCCAAACTCAAGCTGCCCATGCCCAATTTGTTCAGTTGCAAGCTCAAGCTCAGTCTCTTGCCCAGCTGCATAATGCGAATACAAGTAATGTTGGTGGTTCATCACCCTCCGTGGCAACACCTAGCACAGGAACTGCTAAGCGGGCGAACCAGAAGCCACCTTCTCGGCCTCCTGGTTCATCTAGTGCCAACACGGGTTCACCATTTAAAACTATGGAGCTCACCCCAGCTGCTCGTAGAAAGAAGCAAAAGCTACCTGATAAACAAATACCGGACAAAGTGGCTGCACTTCTACCAGAGTCCGGTCTTTATACTCAGTTGCTTGAATTTGAGTCTCGTGTTGATGCTGCTCTGGCAAGGAAGAAGATGGACATCCAAGAGTCCCTTAAATGTCCTCCCCGTGTTCAGAGAACACTTAGAGTTTATGTATTCAACACCTTTGCGAATCAGACGCAAACAAATCCTGAGAAGACAAATGCGGAACCCCCTTCATGGTCACTTAAGATAATTGGGAGATTACTGGAAGACGGCAGGGATCCTGTGGTGGCTGGATTGACACCGAAATCAAGGGCAAAATTTTCATCCCTGTTCAAGAAAGTTACCATATACTTGGACCAAACCCTCTATCCAGATAACCACGTGATTTTATGGGAAAGTTCCCGTTCACCCGCTCTTCATGACGGCTTTGAGGTGAAAAGAAAAGGGGATAAGGAGTTTACTGCAATAGTAAGACTAGAGATGAATTATGTACCTGAGAAATTCAAACTTTCACCAGCTCTCCATGAAGTTCTGGGAATTGAGGTAGAGACCCGACCCAGAATTATAGCTGCTATTTGGCACTATGTCAAGGCCAGGAAGTTGCAGAACCCGAATGACCCTACTTTCTTTGTGTGTGATCCACCTCTTCAGAAGATTTTTGGGGAAGAGAAGATGAAGTTTTCGATGGTTTCACAAAAGATATCACAGCATTTAACTCCACCACAGCCTATACACATAGAGCATAAGATAAAACTTTCAGGAAATTGCCCAGCTGGAACTACCTGCTATGATATACTGGTTGATGTACCCATGGTATTAGAGAAAGAGATGTCTGCATTCTTGGGAAGCACCGAGAGGAGCAAAGAGATTGATGCTTGTGATGAATTGATTTGTGCATCCATAAAAAAAATCCATGAACATCGACGGCGGCGAGCTTTCTTTCTTGGGTTCAGTCAATCTCCTGCAGAGTTTATTAATACTTTGATTGCTTCTCAGAGCAAGGATCTCAAGCTTGTTGCTGGAGATGCTAGTCGTAATGCGGAAAAGGAGCGCCGCTCCGATTTCTACAATCAACCTTGGGCTGAGGATGCTGTTATTCGTTACTTGAACCGAAAGTCAGCCGGAAGTGATGCTCCCGGAAGCACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0008152 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009909 GO:0009987 GO:0010224 GO:0010468 GO:0019222 GO:0022622 GO:0032501 GO:0032502 GO:0033554 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0048364 GO:0048580 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050896 GO:0051239 GO:0051716 GO:0060255 GO:0065007 GO:0071704 GO:0090304 GO:0099402 GO:1901360 GO:2000024 GO:2000026 GO:2000241

KEGG Pathways

Metabolic & Signaling

ko04714 ko05225 map04714 map05225

Pfam Domains

Protein Families

SWIB

Protein Analysis

542

Amino Acids

60.04

Weight (kDa)

9.45

Isoelectric Point (pI)

53.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SWIB	PF02201	325 - 397	1.5e-24	SWIB/MDM2 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013669)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g08160 FvH4_5g08160 FvH4_5g08160
malus_domestica	MD06G1143000.v1.1 MD14G1158200.v1.1
prunus_persica	Prupe.5G152300_v2.0.a1 Prupe.5G152300_v2.0.a1
pyrus_communis	pycom06g13280 pycom14g13180
rosa_chinensis	RchiOBHm_Chr7g0190351
rosa_laevigata	RLG00000004539
rosa_multiflora	Rmu_ssc0000038.1_g000003
rosa_roxburghii	Rroxscaffold_3G00264700
rosa_rugosa	Rorug06G0504000
rosa_samantha	Rh7AG109800 Rh7BG112400 Rh7DG112800
rosa_wichuraiana	Rw7G009530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1268
AccB1I	GGYRCC	1 cut(s)	34
AccBSI	CCGCTC	2 cut(s)	890, 1533
AciI	CCGC	8 cut(s)	62, 170, 361, 698, 888, 1405, 1517, 1531
AclWI	GGATC	4 cut(s)	749, 762, 1109, 1488
AcsI	RAATTY	3 cut(s)	545, 794, 977
AcuI	CTGAAG	1 cut(s)	1109
AcvI	CACGTG	1 cut(s)	859
AfaI	GTAC	2 cut(s)	969, 1284
AfiI	CCNNNNNNNGG	5 cut(s)	47, 128, 347, 521, 711
AflII	CTTAAG	1 cut(s)	719
AgsI	TTSAA	5 cut(s)	545, 652, 811, 982, 1585
AjnI	CCWGG	4 cut(s)	20, 145, 391, 1070
Alw21I	GWGCWC	2 cut(s)	139, 441
Alw26I	GTCTC	3 cut(s)	279, 558, 1019
AlwI	GGATC	4 cut(s)	749, 762, 1109, 1488
AlwNI	CAGNNNCTG	1 cut(s)	353
AoxI	GGCC	2 cut(s)	386, 1068
ApeKI	GCWGC	7 cut(s)	115, 233, 286, 449, 503, 566, 1046
ApoI	RAATTY	3 cut(s)	545, 794, 977
AseI	ATTAAT	1 cut(s)	1452
AspLEI	GCGC	2 cut(s)	178, 1530
AspS9I	GGNCC	1 cut(s)	832
AsuC2I	CCSGG	1 cut(s)	1614
AsuHPI	GGTGA	7 cut(s)	80, 315, 411, 433, 876, 922, 981
AvaII	GGWCC	1 cut(s)	832
BaeGI	GKGCMC	1 cut(s)	153
BamHI	GGATCC	1 cut(s)	754
BanI	GGYRCC	1 cut(s)	34
BanII	GRGCYC	2 cut(s)	139, 441
BauI	CACGAG	1 cut(s)	555
BbrPI	CACGTG	1 cut(s)	859
BbsI	GAAGAC	2 cut(s)	695, 750
Bbv12I	GWGCWC	2 cut(s)	139, 441
BbvCI	CCTCAGC	1 cut(s)	1560
BbvI	GCAGC	7 cut(s)	127, 220, 273, 436, 490, 553, 1033
BccI	CCATC	2 cut(s)	580, 1156
BceAI	ACGGC	3 cut(s)	763, 916, 1418
BciT130I	CCWGG	4 cut(s)	22, 147, 393, 1072
BcnI	CCSGG	1 cut(s)	1614
BcoDI	GTCTC	3 cut(s)	279, 558, 1019
BfaI	CTAG	4 cut(s)	342, 402, 953, 1506
BfmI	CTRYAG	1 cut(s)	1440
BfoI	RGCGCY	1 cut(s)	1531
BfrI	CTTAAG	1 cut(s)	719
BfuAI	ACCTGC	1 cut(s)	1268
BlpI	GCTNAGC	1 cut(s)	357
Bme1390I	CCNGG	5 cut(s)	22, 147, 393, 1072, 1614
Bme18I	GGWCC	1 cut(s)	832
BmgT120I	GGNCC	1 cut(s)	832
BmiI	GGNNCC	3 cut(s)	36, 702, 756
BmrFI	CCNGG	5 cut(s)	22, 147, 393, 1072, 1614
BmsI	GCATC	6 cut(s)	553, 1339, 1382, 1492, 1555, 1597
BpiI	GAAGAC	2 cut(s)	695, 750
BplI	GAGNNNNNCTC	2 cut(s)	1517, 1549
BpmI	CTGGAG	1 cut(s)	1518
Bpu10I	CCTNAGC	1 cut(s)	1560
Bpu1102I	GCTNAGC	1 cut(s)	357
BpuEI	CTTGAG	3 cut(s)	213, 249, 1469
BpuMI	CCSGG	1 cut(s)	1614
BsaAI	YACGTR	1 cut(s)	859
BsaBI	GATNNNNATC	1 cut(s)	590
BsaI	GGTCTC	1 cut(s)	1019
BsaJI	CCNNGG	5 cut(s)	20, 146, 330, 1287, 1553
BsaWI	WCCGGW	2 cut(s)	490, 521
BsaXI	ACNNNNNCTCC	2 cut(s)	981, 1011
Bsc4I	CCNNNNNNNGG	5 cut(s)	47, 128, 347, 521, 711
Bse1I	ACTGG	2 cut(s)	744, 1278
Bse8I	GATNNNNATC	1 cut(s)	590
BseBI	CCWGG	4 cut(s)	22, 147, 393, 1072
BseDI	CCNNGG	5 cut(s)	20, 146, 330, 1287, 1553
BseGI	GGATG	4 cut(s)	591, 800, 1373, 1570
BseJI	GATNNNNATC	1 cut(s)	590
BseLI	CCNNNNNNNGG	5 cut(s)	47, 128, 347, 521, 711
BseMII	CTCAG	7 cut(s)	156, 284, 548, 675, 963, 1484, 1551
BseNI	ACTGG	2 cut(s)	744, 1278
BseRI	GAGGAG	1 cut(s)	1348
BseSI	GKGCMC	1 cut(s)	153
BseXI	GCAGC	7 cut(s)	127, 220, 273, 436, 490, 553, 1033
BseYI	CCCAGC	3 cut(s)	282, 445, 1246
BsgI	GTGCAG	1 cut(s)	489
BshFI	GGCC	2 cut(s)	388, 1070
BshNI	GGYRCC	1 cut(s)	34
BsiHKAI	GWGCWC	2 cut(s)	139, 441
BsiSI	CCGG	4 cut(s)	491, 522, 1599, 1614
BslFI	GGGAC	1 cut(s)	586
BslI	CCNNNNNNNGG	5 cut(s)	47, 128, 347, 521, 711
BsmAI	GTCTC	3 cut(s)	279, 558, 1019
BsmFI	GGGAC	1 cut(s)	586
BsmI	GAATGC	2 cut(s)	211, 1313
BsnI	GGCC	2 cut(s)	388, 1070
Bso31I	GGTCTC	1 cut(s)	1019
Bsp1286I	GDGCHC	3 cut(s)	139, 153, 441
Bsp143I	GATC	3 cut(s)	754, 1114, 1480
Bsp1720I	GCTNAGC	1 cut(s)	357
Bsp19I	CCATGG	1 cut(s)	1287
BspACI	CCGC	8 cut(s)	62, 170, 361, 698, 888, 1405, 1517, 1531
BspANI	GGCC	2 cut(s)	388, 1070
BspCNI	CTCAG	7 cut(s)	157, 283, 547, 676, 964, 1483, 1552
BspHI	TCATGA	1 cut(s)	895
BspLI	GGNNCC	3 cut(s)	36, 702, 756
BspMAI	CTGCAG	1 cut(s)	1444
BspMI	ACCTGC	1 cut(s)	1268
BspPI	GGATC	4 cut(s)	749, 762, 1109, 1488
BspQI	GCTCTTC	1 cut(s)	897
BspT107I	GGYRCC	1 cut(s)	34
BspTI	CTTAAG	1 cut(s)	719
BspTNI	GGTCTC	1 cut(s)	1019
BsrBI	CCGCTC	2 cut(s)	890, 1533
BsrI	ACTGG	2 cut(s)	744, 1278
BssECI	CCNNGG	5 cut(s)	20, 146, 330, 1287, 1553
BssMI	GATC	3 cut(s)	754, 1114, 1480
BssSI	CACGAG	1 cut(s)	555
BssT1I	CCWWGG	2 cut(s)	1287, 1553
Bst2BI	CACGAG	1 cut(s)	555
Bst2UI	CCWGG	4 cut(s)	22, 147, 393, 1072
Bst4CI	ACNGT	1 cut(s)	198
Bst6I	CTCTTC	3 cut(s)	897, 1128, 1137
BstAFI	CTTAAG	1 cut(s)	719
BstBAI	YACGTR	1 cut(s)	859
BstC8I	GCNNGC	5 cut(s)	174, 180, 261, 363, 1410
BstDEI	CTNAG	9 cut(s)	165, 270, 357, 534, 635, 684, 972, 1470, 1560
BstDSI	CCRYGG	2 cut(s)	330, 1287
BstENI	CCTNNNNNAGG	1 cut(s)	345
BstF5I	GGATG	4 cut(s)	591, 800, 1373, 1570
BstH2I	RGCGCY	1 cut(s)	1531
BstHHI	GCGC	2 cut(s)	178, 1530
BstKTI	GATC	3 cut(s)	757, 1117, 1483
BstMAI	GTCTC	3 cut(s)	279, 558, 1019
BstMBI	GATC	3 cut(s)	754, 1114, 1480
BstMWI	GCNNNNNNNGC	6 cut(s)	175, 239, 362, 572, 1502, 1565
BstNI	CCWGG	4 cut(s)	22, 147, 393, 1072
BstSCI	CCNGG	5 cut(s)	20, 145, 391, 1070, 1612
BstSFI	CTRYAG	1 cut(s)	1440
BstSLI	GKGCMC	1 cut(s)	153
BstV1I	GCAGC	7 cut(s)	127, 220, 273, 436, 490, 553, 1033
BstV2I	GAAGAC	2 cut(s)	695, 750
BstX2I	RGATCY	2 cut(s)	754, 1480
BstXI	CCANNNNNNTGG	2 cut(s)	28, 829
BstYI	RGATCY	2 cut(s)	754, 1480
BsuRI	GGCC	2 cut(s)	388, 1070
BtgI	CCRYGG	2 cut(s)	330, 1287
BtsCI	GGATG	4 cut(s)	591, 800, 1373, 1570
BveI	ACCTGC	1 cut(s)	1268
Cac8I	GCNNGC	5 cut(s)	174, 180, 261, 363, 1410
CaiI	CAGNNNCTG	1 cut(s)	353
CciI	TCATGA	1 cut(s)	895
CfoI	GCGC	2 cut(s)	178, 1530
Cfr13I	GGNCC	1 cut(s)	832
CseI	GACGC	1 cut(s)	679
Csp6I	GTAC	2 cut(s)	968, 1283
CviAII	CATG	7 cut(s)	82, 239, 711, 896, 1001, 1288, 1391
CviQI	GTAC	2 cut(s)	968, 1283
DdeI	CTNAG	9 cut(s)	165, 270, 357, 534, 635, 684, 972, 1470, 1560
DpnI	GATC	3 cut(s)	756, 1116, 1482
DpnII	GATC	3 cut(s)	754, 1114, 1480
DraI	TTTAAA	1 cut(s)	427
Eam1104I	CTCTTC	3 cut(s)	897, 1128, 1137
EarI	CTCTTC	3 cut(s)	897, 1128, 1137
Ecl136II	GAGCTC	2 cut(s)	137, 439
Eco130I	CCWWGG	2 cut(s)	1287, 1553
Eco24I	GRGCYC	2 cut(s)	139, 441
Eco31I	GGTCTC	1 cut(s)	1019
Eco32I	GATATC	1 cut(s)	1179
Eco47I	GGWCC	1 cut(s)	832
Eco53kI	GAGCTC	2 cut(s)	137, 439
Eco57I	CTGAAG	1 cut(s)	1109
Eco72I	CACGTG	1 cut(s)	859
EcoICRI	GAGCTC	2 cut(s)	137, 439
EcoNI	CCTNNNNNAGG	1 cut(s)	345
EcoRII	CCWGG	4 cut(s)	20, 145, 391, 1070
EcoRV	GATATC	1 cut(s)	1179
EcoT14I	CCWWGG	2 cut(s)	1287, 1553
EcoT38I	GRGCYC	2 cut(s)	139, 441
ErhI	CCWWGG	2 cut(s)	1287, 1553
FaeI	CATG	7 cut(s)	85, 242, 714, 899, 1004, 1291, 1394
FalI	AAGNNNNNCTT	2 cut(s)	588, 620
FaqI	GGGAC	1 cut(s)	586
FatI	CATG	7 cut(s)	81, 238, 710, 895, 1000, 1287, 1390
FauI	CCCGC	2 cut(s)	354, 895
FokI	GGATG	4 cut(s)	578, 787, 1360, 1577
FriOI	GRGCYC	2 cut(s)	139, 441
FspBI	CTAG	4 cut(s)	342, 402, 953, 1506
GlaI	GCGC	2 cut(s)	177, 1529
GsaI	CCCAGC	3 cut(s)	286, 449, 1250
GsuI	CTGGAG	1 cut(s)	1518
HaeII	RGCGCY	1 cut(s)	1531
HaeIII	GGCC	2 cut(s)	388, 1070
HapII	CCGG	4 cut(s)	491, 522, 1599, 1614
HgaI	GACGC	1 cut(s)	679
HhaI	GCGC	2 cut(s)	178, 1530
Hin1II	CATG	7 cut(s)	85, 242, 714, 899, 1004, 1291, 1394
Hin6I	GCGC	2 cut(s)	176, 1528
HinP1I	GCGC	2 cut(s)	176, 1528
HindIII	AAGCTT	1 cut(s)	1487
HinfI	GANTC	5 cut(s)	85, 518, 551, 599, 664
HpaII	CCGG	4 cut(s)	491, 522, 1599, 1614
HphI	GGTGA	7 cut(s)	80, 315, 411, 433, 876, 922, 981
Hpy166II	GTNNAC	3 cut(s)	419, 884, 936
Hpy188I	TCNGA	7 cut(s)	123, 166, 627, 669, 1128, 1473, 1537
Hpy188III	TCNNGA	5 cut(s)	683, 811, 848, 896, 1236
Hpy8I	GTNNAC	3 cut(s)	419, 884, 936
Hpy99I	CGWCG	1 cut(s)	1404
HpyAV	CCTTC	2 cut(s)	390, 717
HpyCH4III	ACNGT	1 cut(s)	198
HpyCH4IV	ACGT	1 cut(s)	858
HpyCH4V	TGCA	9 cut(s)	188, 259, 289, 506, 941, 1081, 1313, 1373, 1442
HpyF10VI	GCNNNNNNNGC	6 cut(s)	175, 239, 362, 572, 1502, 1565
HpyF3I	CTNAG	9 cut(s)	165, 270, 357, 534, 635, 684, 972, 1470, 1560
HpySE526I	ACGT	1 cut(s)	858
Hsp92II	CATG	7 cut(s)	85, 242, 714, 899, 1004, 1291, 1394
HspAI	GCGC	2 cut(s)	176, 1528
Kzo9I	GATC	3 cut(s)	754, 1114, 1480
LguI	GCTCTTC	1 cut(s)	897
LmnI	GCTCC	6 cut(s)	134, 436, 1335, 1525, 1538, 1615
Lsp1109I	GCAGC	7 cut(s)	127, 220, 273, 436, 490, 553, 1033
LweI	GCATC	6 cut(s)	553, 1339, 1382, 1492, 1555, 1597
MaeI	CTAG	4 cut(s)	342, 402, 953, 1506
MaeII	ACGT	1 cut(s)	858
MaeIII	GTNAC	3 cut(s)	714, 817, 1577
MalI	GATC	3 cut(s)	756, 1116, 1482
MbiI	CCGCTC	2 cut(s)	890, 1533
MboI	GATC	3 cut(s)	754, 1114, 1480
MflI	RGATCY	2 cut(s)	754, 1480
MhlI	GDGCHC	3 cut(s)	139, 153, 441
MlyI	GAGTC	3 cut(s)	527, 560, 608
MmeI	TCCRAC	1 cut(s)	146
MseI	TTAA	5 cut(s)	426, 606, 720, 1190, 1452
MspA1I	CMGCKG	3 cut(s)	286, 449, 1250
MspCI	CTTAAG	1 cut(s)	719
MspI	CCGG	4 cut(s)	491, 522, 1599, 1614
MspR9I	CCNGG	5 cut(s)	22, 147, 393, 1072, 1614
Mva1269I	GAATGC	2 cut(s)	211, 1313
MvaI	CCWGG	4 cut(s)	22, 147, 393, 1072
MwoI	GCNNNNNNNGC	6 cut(s)	175, 239, 362, 572, 1502, 1565
NciI	CCSGG	1 cut(s)	1614
NcoI	CCATGG	1 cut(s)	1287
NdeII	GATC	3 cut(s)	754, 1114, 1480
NlaIII	CATG	7 cut(s)	85, 242, 714, 899, 1004, 1291, 1394
NlaIV	GGNNCC	3 cut(s)	36, 702, 756
NmeAIII	GCCGAG	1 cut(s)	364
NmuCI	GTSAC	1 cut(s)	714
PagI	TCATGA	1 cut(s)	895
PciSI	GCTCTTC	1 cut(s)	897
PcsI	WCGNNNNNNNCGW	2 cut(s)	119, 1405
PctI	GAATGC	2 cut(s)	211, 1313
PfeI	GAWTC	2 cut(s)	85, 664
PfoI	TCCNGGA	1 cut(s)	1612
PleI	GAGTC	3 cut(s)	526, 559, 607
PmaCI	CACGTG	1 cut(s)	859
PmlI	CACGTG	1 cut(s)	859
PpsI	GAGTC	3 cut(s)	526, 559, 607
Ppu21I	YACGTR	1 cut(s)	859
PshBI	ATTAAT	1 cut(s)	1452
Psp124BI	GAGCTC	2 cut(s)	139, 441
Psp6I	CCWGG	4 cut(s)	20, 145, 391, 1070
PspCI	CACGTG	1 cut(s)	859
PspFI	CCCAGC	3 cut(s)	282, 445, 1246
PspGI	CCWGG	4 cut(s)	20, 145, 391, 1070
PspN4I	GGNNCC	3 cut(s)	36, 702, 756
PspPI	GGNCC	1 cut(s)	832
PstI	CTGCAG	1 cut(s)	1444
PstNI	CAGNNNCTG	1 cut(s)	353
PsuI	RGATCY	2 cut(s)	754, 1480
PvuII	CAGCTG	3 cut(s)	286, 449, 1250
RsaI	GTAC	2 cut(s)	969, 1284
RsaNI	GTAC	2 cut(s)	968, 1283
SacI	GAGCTC	2 cut(s)	139, 441
SapI	GCTCTTC	1 cut(s)	897
SaqAI	TTAA	5 cut(s)	426, 606, 720, 1190, 1452
Sau3AI	GATC	3 cut(s)	754, 1114, 1480
Sau96I	GGNCC	1 cut(s)	832
SchI	GAGTC	3 cut(s)	527, 560, 608
ScrFI	CCNGG	5 cut(s)	22, 147, 393, 1072, 1614
SduI	GDGCHC	3 cut(s)	139, 153, 441
SfaNI	GCATC	6 cut(s)	553, 1339, 1382, 1492, 1555, 1597
SfcI	CTRYAG	1 cut(s)	1440
SinI	GGWCC	1 cut(s)	832
SmlI	CTYRAG	4 cut(s)	228, 264, 719, 1484
SmoI	CTYRAG	4 cut(s)	228, 264, 719, 1484
SsiI	CCGC	8 cut(s)	62, 170, 361, 698, 888, 1405, 1517, 1531
SspMI	CTAG	4 cut(s)	342, 402, 953, 1506
SstI	GAGCTC	2 cut(s)	139, 441
StyD4I	CCNGG	5 cut(s)	20, 145, 391, 1070, 1612
StyI	CCWWGG	2 cut(s)	1287, 1553
TaaI	ACNGT	1 cut(s)	198
TaiI	ACGT	1 cut(s)	861
TaqI	TCGA	2 cut(s)	1160, 1399
TauI	GCSGC	3 cut(s)	172, 1408, 1533
TfiI	GAWTC	2 cut(s)	85, 664
Tru1I	TTAA	5 cut(s)	426, 606, 720, 1190, 1452
Tru9I	TTAA	5 cut(s)	426, 606, 720, 1190, 1452
TseFI	GTSAC	1 cut(s)	714
TseI	GCWGC	7 cut(s)	115, 233, 286, 449, 503, 566, 1046
Tsp45I	GTSAC	1 cut(s)	714
TspGWI	ACGGA	1 cut(s)	319
Vha464I	CTTAAG	1 cut(s)	719
VpaK11BI	GGWCC	1 cut(s)	832
VspI	ATTAAT	1 cut(s)	1452
XagI	CCTNNNNNAGG	1 cut(s)	345
XapI	RAATTY	3 cut(s)	545, 794, 977
XspI	CTAG	4 cut(s)	342, 402, 953, 1506

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.