Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0013125

Physical Location & Seq

Genomic Coordinates Reverse (-)

28805171 .. 28809498

Gene Structure 4328 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1145400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1461 bp

ATGGCAAAGCAAGAACCAAACTCCGTCGTCTCTGATCTCATCGGCTTCCTAAACGCCGCCCCCACCGCATTCCACGCCGTCGACGAGGCGAAGAAGCGGCTGCGAAGCGCGGGCTACGAGCAAGTTTTGGAGAGGGAGGATTGGAAATTGGAGGCTGGTAAAAAGTATTTCTTCACCAGGAATTACTCCACCATTGTTGCTTTCGCAATCGGTAAAAAGTATGTTGCTGGAAACGGATTTCATATAGTTGGTGCTCATACTGATAGTCCTTGTCTCAAATTGAAACCGGTTTCCAAGGTAGCTAAAGGCGGGTACTTGGAAGTGGGTGTCCAAACATATGGCGGTGGCTTGTGGCATACATGGTTCGACCGTGACTTGACAATTGCTGGAAGGGTGATAGTAAGAGAAGAAAAGGATGGCTCTGTTTTGTATTCACATAAACTTGTTAGAATTGAGGAGCCCATAATGCGGATCCCTACTTTGGCAATTCACTTAGACAGGGATGTTAGAGATGCATTCAAGGTGAATGCGCAGACGCATCTTCTTCCTGTCTTGGCAACGGCAGTCAAGGCAGAGCTCAATAAAGTGGTAGCTGAAGATGGTGAGGCTGATAATGATATTCAGACATCTGGAAAGAAATCTAATGAGAAAACAACCTCAAATGCCTTGAAGCATCATTCACTTCTATTACAGCTACTTGCAGATCAGCTTGGATGTGAACCGGAACATATCTCTGATTTTGAATTGCAAGCGTGTGATACTCAACCAAGCATAGTAGCCGGTGCAGCAAAAGAATTTGTTTTCTCAGGAAGGCTTGATAATCTTTGCATGTCGTTTTGTTCTCTAAAGGCATTGATAGACGCAACATCTTCTGATAGCAACCTTGAAGATGAGTCCGGCGTTAGAATGGTGGCTTTGTTTGATCATGAGGAGGTTGGATCTAATTCCGCCCAAGGGGCTGGTTCTCCAGCCATGTTAAATACTCTTTCACGAATTACACACTCCTTCACCACAGATTCTAAGCTGCTCGAGAAAGCTATTCAGAGGAGCTTTCTTGTATCTGCTGACATGGCACATGCCTTGCATCCTAATTATATGGACAAACATGAACAGAATCACCAGCCCAAGTTGCATGGCGGACTTGTGATCAAACACAATGCAAATCAACGCTATGCAACCAATGCTGTCACTGCATTAATATTCAGGGAGATAGCAAGGAATCATAGCCTTCCTGTCCAGGACTTTGTGGTTCGTAATGACATGGCATGCGGTTCAACCATCGGTCCCATTCTTGCAAGTGGAGTGGGAATACGAACAGTTGATGTAGGTGCGCCGCAGCTGTCAATGCACAGCATACGAGAAATGTGTGCTGTTGATGATGTGAAGCATTCCTACGAGCATTTCAAAGCCTTTTTCCAGGAGTTCACTCGTCTGGATTCCAAGATTAAAGTGGATGCGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004177 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006508 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008235 GO:0008237 GO:0008238 GO:0009987 GO:0016787 GO:0019538 GO:0034641 GO:0042802 GO:0043170 GO:0043603 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0070006 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_M18

Protein Analysis

487

Amino Acids

53.5

Weight (kDa)

6.43

Isoelectric Point (pI)

32.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M18	PF02127	16 - 472	3.6e-163	Aminopeptidase I zinc metalloprotease (M18)
Peptidase_M42	PF05343	343 - 464	4.1e-06	M42 glutamyl aminopeptidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013125)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G60160
fragaria_vesca	FvH4_5g07860 FvH4_5g07860 FvH4_5g07860
malus_domestica	MD06G1145400.v1.1 MD14G1160100.v1.1
prunus_persica	Prupe.5G153700_v2.0.a1
pyrus_communis	pycom06g13470 pycom14g13360
rosa_chinensis	RchiOBHm_Chr7g0190601
rosa_laevigata	RLG00000004511
rosa_roxburghii	Rroxscaffold_3G00264470
rosa_rugosa	Rorug06G0505600
rosa_samantha	Rh7AG112200 Rh7BG115000 Rh7CG117100 Rh7DG116200
rosa_wichuraiana	Rw7G009790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	531
AccI	GTMKAC	1 cut(s)	81
AccII	CGCG	1 cut(s)	110
AclWI	GGATC	3 cut(s)	466, 479, 946
AcsI	RAATTY	1 cut(s)	794
AcuI	CTGAAG	1 cut(s)	615
AfaI	GTAC	1 cut(s)	314
AfiI	CCNNNNNNNGG	3 cut(s)	468, 481, 954
AgeI	ACCGGT	1 cut(s)	286
AgsI	TTSAA	7 cut(s)	283, 520, 670, 743, 887, 1275, 1405
AjnI	CCWGG	3 cut(s)	176, 1236, 1416
AluBI	AGCT	9 cut(s)	302, 577, 593, 694, 709, 1024, 1037, 1050, 1339
AluI	AGCT	9 cut(s)	302, 577, 593, 694, 709, 1024, 1037, 1050, 1339
Alw21I	GWGCWC	2 cut(s)	256, 579
Alw26I	GTCTC	2 cut(s)	34, 278
AlwI	GGATC	3 cut(s)	466, 479, 946
Ama87I	CYCGRG	1 cut(s)	1028
ApeKI	GCWGC	4 cut(s)	100, 785, 1024, 1336
ApoI	RAATTY	1 cut(s)	794
AseI	ATTAAT	1 cut(s)	1196
AsiGI	ACCGGT	1 cut(s)	286
AspLEI	GCGC	3 cut(s)	110, 532, 1333
AspS9I	GGNCC	1 cut(s)	1283
AsuHPI	GGTGA	6 cut(s)	166, 406, 535, 614, 1000, 1109
AvaI	CYCGRG	1 cut(s)	1028
AvaII	GGWCC	1 cut(s)	1283
BamHI	GGATCC	1 cut(s)	471
BanII	GRGCYC	2 cut(s)	462, 579
BarI	GAAGNNNNNNTAC	2 cut(s)	1376, 1408
Bbv12I	GWGCWC	2 cut(s)	256, 579
BbvI	GCAGC	4 cut(s)	87, 797, 1011, 1348
BccI	CCATC	3 cut(s)	410, 593, 1286
BceAI	ACGGC	2 cut(s)	62, 576
BciT130I	CCWGG	3 cut(s)	178, 1238, 1418
BclI	TGATCA	2 cut(s)	922, 1146
BcoDI	GTCTC	2 cut(s)	34, 278
BglI	GCCNNNNNGGC	1 cut(s)	956
BisI	GCNGC	7 cut(s)	57, 98, 101, 786, 1025, 1334, 1337
BlsI	GCNGC	7 cut(s)	58, 99, 102, 787, 1026, 1335, 1338
Bme1390I	CCNGG	3 cut(s)	178, 1238, 1418
Bme18I	GGWCC	1 cut(s)	1283
BmeT110I	CYCGRG	1 cut(s)	1028
BmgT120I	GGNCC	1 cut(s)	1283
BmiI	GGNNCC	3 cut(s)	459, 473, 1285
BmrFI	CCNGG	3 cut(s)	178, 1238, 1418
BmsI	GCATC	5 cut(s)	502, 547, 682, 1093, 1444
BpmI	CTGGAG	1 cut(s)	951
BsaJI	CCNNGG	2 cut(s)	294, 952
BsaWI	WCCGGW	2 cut(s)	286, 721
Bsc4I	CCNNNNNNNGG	3 cut(s)	468, 481, 954
Bse118I	RCCGGY	2 cut(s)	286, 779
BseBI	CCWGG	3 cut(s)	178, 1238, 1418
BseDI	CCNNGG	2 cut(s)	294, 952
BseGI	GGATG	5 cut(s)	421, 508, 719, 1084, 1459
BseLI	CCNNNNNNNGG	3 cut(s)	468, 481, 954
BseMII	CTCAG	1 cut(s)	819
BseRI	GAGGAG	3 cut(s)	470, 944, 1060
BseXI	GCAGC	4 cut(s)	87, 797, 1011, 1348
BsgI	GTGCAG	1 cut(s)	804
Bsh1236I	CGCG	1 cut(s)	110
Bsh1285I	CGRYCG	1 cut(s)	370
BshTI	ACCGGT	1 cut(s)	286
BsiEI	CGRYCG	1 cut(s)	370
BsiHKAI	GWGCWC	2 cut(s)	256, 579
BsiHKCI	CYCGRG	1 cut(s)	1028
BsiSI	CCGG	4 cut(s)	287, 722, 780, 897
BslFI	GGGAC	1 cut(s)	1269
BslI	CCNNNNNNNGG	3 cut(s)	468, 481, 954
BsmAI	GTCTC	2 cut(s)	34, 278
BsmBI	CGTCTC	1 cut(s)	34
BsmFI	GGGAC	1 cut(s)	1269
BsmI	GAATGC	4 cut(s)	68, 515, 532, 1387
BsoBI	CYCGRG	1 cut(s)	1028
Bsp1286I	GDGCHC	3 cut(s)	256, 462, 579
Bsp143I	GATC	6 cut(s)	34, 471, 703, 922, 938, 1146
BspCNI	CTCAG	1 cut(s)	818
BspFNI	CGCG	1 cut(s)	110
BspHI	TCATGA	1 cut(s)	925
BspLI	GGNNCC	3 cut(s)	459, 473, 1285
BspPI	GGATC	3 cut(s)	466, 479, 946
BsrFI	RCCGGY	2 cut(s)	286, 779
BssAI	RCCGGY	2 cut(s)	286, 779
BssECI	CCNNGG	2 cut(s)	294, 952
BssMI	GATC	6 cut(s)	34, 471, 703, 922, 938, 1146
BssT1I	CCWWGG	2 cut(s)	294, 952
Bst2UI	CCWGG	3 cut(s)	178, 1238, 1418
Bst4CI	ACNGT	2 cut(s)	371, 1318
BstAPI	GCANNNNNTGC	1 cut(s)	1181
BstC8I	GCNNGC	3 cut(s)	112, 750, 1267
BstDEI	CTNAG	3 cut(s)	493, 805, 1020
BstF5I	GGATG	5 cut(s)	421, 508, 719, 1084, 1459
BstFNI	CGCG	1 cut(s)	110
BstHHI	GCGC	3 cut(s)	110, 532, 1333
BstKTI	GATC	6 cut(s)	37, 474, 706, 925, 941, 1149
BstMAI	GTCTC	2 cut(s)	34, 278
BstMBI	GATC	6 cut(s)	34, 471, 703, 922, 938, 1146
BstMCI	CGRYCG	1 cut(s)	370
BstNI	CCWGG	3 cut(s)	178, 1238, 1418
BstNSI	RCATGY	3 cut(s)	832, 1079, 1269
BstSCI	CCNGG	3 cut(s)	176, 1236, 1416
BstUI	CGCG	1 cut(s)	110
BstV1I	GCAGC	4 cut(s)	87, 797, 1011, 1348
BstX2I	RGATCY	2 cut(s)	471, 938
BstXI	CCANNNNNNTGG	2 cut(s)	338, 959
BstYI	RGATCY	2 cut(s)	471, 938
BtsCI	GGATG	5 cut(s)	421, 508, 719, 1084, 1459
BtsI	GCAGTG	1 cut(s)	1188
BtsIMutI	CAGTG	1 cut(s)	1188
Cac8I	GCNNGC	3 cut(s)	112, 750, 1267
CciI	TCATGA	1 cut(s)	925
CfoI	GCGC	3 cut(s)	110, 532, 1333
Cfr10I	RCCGGY	2 cut(s)	286, 779
Cfr13I	GGNCC	1 cut(s)	1283
CseI	GACGC	2 cut(s)	544, 869
Csp6I	GTAC	1 cut(s)	313
CspAI	ACCGGT	1 cut(s)	286
CspCI	CAANNNNNGTGG	4 cut(s)	178, 213, 1284, 1319
CviQI	GTAC	1 cut(s)	313
DdeI	CTNAG	3 cut(s)	493, 805, 1020
DpnI	GATC	6 cut(s)	36, 473, 705, 924, 940, 1148
DpnII	GATC	6 cut(s)	34, 471, 703, 922, 938, 1146
EciI	GGCGGA	2 cut(s)	937, 1152
Ecl136II	GAGCTC	1 cut(s)	577
Eco130I	CCWWGG	2 cut(s)	294, 952
Eco24I	GRGCYC	2 cut(s)	462, 579
Eco47I	GGWCC	1 cut(s)	1283
Eco53kI	GAGCTC	1 cut(s)	577
Eco57I	CTGAAG	1 cut(s)	615
Eco88I	CYCGRG	1 cut(s)	1028
EcoICRI	GAGCTC	1 cut(s)	577
EcoRII	CCWGG	3 cut(s)	176, 1236, 1416
EcoT14I	CCWWGG	2 cut(s)	294, 952
EcoT22I	ATGCAT	1 cut(s)	517
EcoT38I	GRGCYC	2 cut(s)	462, 579
ErhI	CCWWGG	2 cut(s)	294, 952
Esp3I	CGTCTC	1 cut(s)	34
FalI	AAGNNNNNCTT	2 cut(s)	155, 187
FaqI	GGGAC	1 cut(s)	1269
FauI	CCCGC	2 cut(s)	103, 302
FauNDI	CATATG	1 cut(s)	337
FbaI	TGATCA	2 cut(s)	922, 1146
FblI	GTMKAC	1 cut(s)	81
Fnu4HI	GCNGC	7 cut(s)	57, 98, 101, 786, 1025, 1334, 1337
FokI	GGATG	4 cut(s)	428, 515, 726, 1071
FriOI	GRGCYC	2 cut(s)	462, 579
Fsp4HI	GCNGC	7 cut(s)	57, 98, 101, 786, 1025, 1334, 1337
FspI	TGCGCA	1 cut(s)	531
GlaI	GCGC	3 cut(s)	109, 531, 1332
GluI	GCNGC	7 cut(s)	57, 98, 101, 786, 1025, 1334, 1337
GsuI	CTGGAG	1 cut(s)	951
HapII	CCGG	4 cut(s)	287, 722, 780, 897
HgaI	GACGC	2 cut(s)	544, 869
HhaI	GCGC	3 cut(s)	110, 532, 1333
Hin6I	GCGC	3 cut(s)	108, 530, 1331
HinP1I	GCGC	3 cut(s)	108, 530, 1331
HincII	GTYRAC	1 cut(s)	82
HindII	GTYRAC	1 cut(s)	82
HinfI	GANTC	5 cut(s)	893, 1016, 1114, 1219, 1436
HpaII	CCGG	4 cut(s)	287, 722, 780, 897
HphI	GGTGA	6 cut(s)	166, 406, 535, 614, 1000, 1109
Hpy166II	GTNNAC	3 cut(s)	82, 719, 1425
Hpy188I	TCNGA	5 cut(s)	34, 624, 736, 874, 1044
Hpy188III	TCNNGA	6 cut(s)	630, 807, 926, 990, 1030, 1433
Hpy8I	GTNNAC	3 cut(s)	82, 719, 1425
Hpy99I	CGWCG	3 cut(s)	29, 83, 86
HpyAV	CCTTC	4 cut(s)	384, 804, 1015, 1238
HpyCH4III	ACNGT	2 cut(s)	371, 1318
HpyF3I	CTNAG	3 cut(s)	493, 805, 1020
HspAI	GCGC	3 cut(s)	108, 530, 1331
Ksp22I	TGATCA	2 cut(s)	922, 1146
Kzo9I	GATC	6 cut(s)	34, 471, 703, 922, 938, 1146
LmnI	GCTCC	2 cut(s)	457, 1047
Lsp1109I	GCAGC	4 cut(s)	87, 797, 1011, 1348
LweI	GCATC	5 cut(s)	502, 547, 682, 1093, 1444
MaeIII	GTNAC	2 cut(s)	371, 1186
MalI	GATC	6 cut(s)	36, 473, 705, 924, 940, 1148
MboI	GATC	6 cut(s)	34, 471, 703, 922, 938, 1146
MboII	GAAGA	8 cut(s)	103, 163, 419, 533, 536, 608, 861, 899
MfeI	CAATTG	1 cut(s)	381
MflI	RGATCY	2 cut(s)	471, 938
MhlI	GDGCHC	3 cut(s)	256, 462, 579
MlyI	GAGTC	1 cut(s)	902
MmeI	TCCRAC	1 cut(s)	916
Mph1103I	ATGCAT	1 cut(s)	517
MseI	TTAA	3 cut(s)	977, 1196, 1446
MspA1I	CMGCKG	1 cut(s)	1339
MspI	CCGG	4 cut(s)	287, 722, 780, 897
MspR9I	CCNGG	3 cut(s)	178, 1238, 1418
MunI	CAATTG	1 cut(s)	381
Mva1269I	GAATGC	4 cut(s)	68, 515, 532, 1387
MvaI	CCWGG	3 cut(s)	178, 1238, 1418
MvnI	CGCG	1 cut(s)	110
NdeI	CATATG	1 cut(s)	337
NdeII	GATC	6 cut(s)	34, 471, 703, 922, 938, 1146
NlaIV	GGNNCC	3 cut(s)	459, 473, 1285
NmuCI	GTSAC	2 cut(s)	371, 1186
NsbI	TGCGCA	1 cut(s)	531
NsiI	ATGCAT	1 cut(s)	517
NspI	RCATGY	3 cut(s)	832, 1079, 1269
PaeI	GCATGC	1 cut(s)	1269
PaeR7I	CTCGAG	1 cut(s)	1028
PagI	TCATGA	1 cut(s)	925
PcsI	WCGNNNNNNNCGW	1 cut(s)	81
PctI	GAATGC	4 cut(s)	68, 515, 532, 1387
PfeI	GAWTC	4 cut(s)	1016, 1114, 1219, 1436
PfoI	TCCNGGA	2 cut(s)	1236, 1416
PinAI	ACCGGT	1 cut(s)	286
PkrI	GCNGC	7 cut(s)	58, 99, 102, 787, 1026, 1335, 1338
PleI	GAGTC	1 cut(s)	901
PpsI	GAGTC	1 cut(s)	901
PshBI	ATTAAT	1 cut(s)	1196
Psp124BI	GAGCTC	1 cut(s)	579
Psp6I	CCWGG	3 cut(s)	176, 1236, 1416
PspGI	CCWGG	3 cut(s)	176, 1236, 1416
PspN4I	GGNNCC	3 cut(s)	459, 473, 1285
PspPI	GGNCC	1 cut(s)	1283
PsuI	RGATCY	2 cut(s)	471, 938
PvuII	CAGCTG	1 cut(s)	1339
RsaI	GTAC	1 cut(s)	314
RsaNI	GTAC	1 cut(s)	313
SacI	GAGCTC	1 cut(s)	579
SalI	GTCGAC	1 cut(s)	80
SaqAI	TTAA	3 cut(s)	977, 1196, 1446
SatI	GCNGC	7 cut(s)	57, 98, 101, 786, 1025, 1334, 1337
Sau3AI	GATC	6 cut(s)	34, 471, 703, 922, 938, 1146
Sau96I	GGNCC	1 cut(s)	1283
SchI	GAGTC	1 cut(s)	902
ScrFI	CCNGG	3 cut(s)	178, 1238, 1418
SduI	GDGCHC	3 cut(s)	256, 462, 579
SfaNI	GCATC	5 cut(s)	502, 547, 682, 1093, 1444
Sfr274I	CTCGAG	1 cut(s)	1028
SgrDI	CGTCGACG	1 cut(s)	80
SinI	GGWCC	1 cut(s)	1283
SlaI	CTCGAG	1 cut(s)	1028
SmlI	CTYRAG	1 cut(s)	1028
SmoI	CTYRAG	1 cut(s)	1028
SphI	GCATGC	1 cut(s)	1269
SspI	AATATT	1 cut(s)	1200
SstI	GAGCTC	1 cut(s)	579
StyD4I	CCNGG	3 cut(s)	176, 1236, 1416
StyI	CCWWGG	2 cut(s)	294, 952
TaaI	ACNGT	2 cut(s)	371, 1318
TaqI	TCGA	3 cut(s)	81, 366, 1029
TaqII	GACCGA	1 cut(s)	1271
TauI	GCSGC	3 cut(s)	59, 100, 1336
TfiI	GAWTC	4 cut(s)	1016, 1114, 1219, 1436
Tru1I	TTAA	3 cut(s)	977, 1196, 1446
Tru9I	TTAA	3 cut(s)	977, 1196, 1446
TscAI	CASTG	1 cut(s)	1195
TseFI	GTSAC	2 cut(s)	371, 1186
TseI	GCWGC	4 cut(s)	100, 785, 1024, 1336
Tsp45I	GTSAC	2 cut(s)	371, 1186
TspDTI	ATGAA	2 cut(s)	230, 1122
TspGWI	ACGGA	2 cut(s)	13, 249
TspRI	CASTG	1 cut(s)	1195
VpaK11BI	GGWCC	1 cut(s)	1283
VspI	ATTAAT	1 cut(s)	1196
XapI	RAATTY	1 cut(s)	794
XceI	RCATGY	3 cut(s)	832, 1079, 1269
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1447
XhoI	CTCGAG	1 cut(s)	1028
XmiI	GTMKAC	1 cut(s)	81
Zsp2I	ATGCAT	1 cut(s)	517

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.