Rh7DG116200

Belongs to the peptidase M18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0013125
Physical Location & Seq
Reverse (-)
9807394 .. 9812760
5367 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG116200.1

Sequence Viewer

Length: 1014 bp
ATGGCAAAGCAAGAATCAAACTGTGAAGGAAGCTCAGTCGTCTCTGACCTCATCAACTTCCTCAACGCTTGTCCCACTGCTTTTCACGCCGTCGACGAGGCGAAGAAGCGTCTGCAAATCGCGGGGTACGAGCAGGTTTTGGAGAGAGAGGATTGGAAATTGGAGGCTGGTAAAAAGTACTACTTCACCAGGAACTACTCCACCATTGTGGCTTTTGCAATCGGTAAAAAATTTGTTGCCGGAAATGGATTCCATATAGTTGGTGCTCATACTGATAGTCCTTGTCTCAAATTGAAGCCTGTTTCCAAGGTAGCTAAGGGTGGGTACTTGGAAGTGGGAGTCCAAACATATGGAGGTGGCTTGTGGCATACCTGGTTCGACCGTGACTTGACAATTGCAGGAAGGGTGATAGTAAGAGAAGAGAAAGATGGCGTTGTTTCTTACTCACATAGACTTGTCCGGATTGAGGAGCCCATCATGCGGATTCCTACTCTGGCAATTCACCTGGACAGGGATGTTAGAGAGGCGTTTAAGGTGAATGCACAAAGTCACCTTCTTCCTGTCTTGGCAACAGCAGTCAAGGCAGAGCTCAATAAAGTAGTTGCAGAAAGTGGCCAATCTAAGGAAACAACCTCAATTAACTCAAAGCATCACTCGCTTCTGTTACAGCTGCTTGCAGATAAGCTTGGGTGTGAACCAGAACATATAAGTGATTTTGAATTGCAAGCATGTGATACTCAACCAAGTTTAGTAGCCGGTGCAGCAAAAGAATTTATTTTCTCAGGAAGGCTTGATAATCTCTGCATGTCATTTTGTTCCCTAAAGGCATTGATAGACGCAACAGCTCCTGAAAGCAACCTTGAGGATGAGACTGGTGTTAGAATGGTGGCTTTGTTTGATCACGAGGAGGTTGGATCTGACTCCGCCCAAGGGGCTGGGTCTCCAGCCATGTTGAATACTCTTTCACGAATTACACATTCCTTTACCACAGATTCTAAGGTACTGACTCTTTGA

Protein Analysis

337

Amino Acids

36.92

Weight (kDa)

5.96

Isoelectric Point (pI)

38.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M18 PF02127 20 - 331 2.1e-102 Aminopeptidase I zinc metalloprotease (M18)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013125)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G60160
fragaria_vesca FvH4_5g07860 FvH4_5g07860 FvH4_5g07860
malus_domestica MD06G1145400.v1.1 MD14G1160100.v1.1
prunus_persica Prupe.5G153700_v2.0.a1
pyrus_communis pycom06g13470 pycom14g13360
rosa_chinensis RchiOBHm_Chr7g0190601
rosa_laevigata RLG00000004511
rosa_roxburghii Rroxscaffold_3G00264470
rosa_rugosa Rorug06G0505600
rosa_samantha Rh7AG112200 Rh7BG115000 Rh7CG117100 Rh7DG116200
rosa_wichuraiana Rw7G009790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 124
AccI GTMKAC 1 cut(s) 93
AccII CGCG 1 cut(s) 122
AccIII TCCGGA 1 cut(s) 459
AciI CCGC 3 cut(s) 122, 481, 924
AclWI GGATC 1 cut(s) 922
AcoI YGGCCR 1 cut(s) 613
AcsI RAATTY 2 cut(s) 230, 770
AfaI GTAC 4 cut(s) 128, 179, 326, 1002
AfiI CCNNNNNNNGG 5 cut(s) 466, 480, 511, 622, 930
AgsI TTSAA 3 cut(s) 295, 719, 955
AjnI CCWGG 3 cut(s) 188, 371, 504
AleI CACNNNNGTG 1 cut(s) 206
AluBI AGCT 6 cut(s) 33, 314, 589, 670, 685, 845
AluI AGCT 6 cut(s) 33, 314, 589, 670, 685, 845
Alw21I GWGCWC 2 cut(s) 268, 591
Alw26I GTCTC 4 cut(s) 46, 290, 863, 945
AlwI GGATC 1 cut(s) 922
AlwNI CAGNNNCTG 1 cut(s) 848
Aor13HI TCCGGA 1 cut(s) 459
AoxI GGCC 1 cut(s) 613
ApeKI GCWGC 2 cut(s) 670, 761
ApoI RAATTY 2 cut(s) 230, 770
AsuHPI GGTGA 5 cut(s) 178, 418, 494, 542, 547
BalI TGGCCA 1 cut(s) 615
BanII GRGCYC 2 cut(s) 474, 591
BauI CACGAG 1 cut(s) 902
Bbv12I GWGCWC 2 cut(s) 268, 591
BbvI GCAGC 2 cut(s) 657, 773
BccI CCATC 2 cut(s) 422, 482
BceAI ACGGC 1 cut(s) 74
BciT130I CCWGG 3 cut(s) 190, 373, 506
BclI TGATCA 1 cut(s) 898
BcoDI GTCTC 4 cut(s) 46, 290, 863, 945
BfuAI ACCTGC 1 cut(s) 124
BglI GCCNNNNNGGC 1 cut(s) 932
BisI GCNGC 2 cut(s) 671, 762
BlsI GCNGC 2 cut(s) 672, 763
BmcAI AGTACT 1 cut(s) 179
Bme1390I CCNGG 3 cut(s) 190, 373, 506
BmiI GGNNCC 1 cut(s) 471
BmrFI CCNGG 3 cut(s) 190, 373, 506
BmsI GCATC 1 cut(s) 658
BpmI CTGGAG 1 cut(s) 927
Bpu10I CCTNAGC 1 cut(s) 315
BpuEI CTTGAG 1 cut(s) 881
BsaI GGTCTC 1 cut(s) 945
BsaJI CCNNGG 2 cut(s) 306, 928
BsaWI WCCGGW 1 cut(s) 459
Bsc4I CCNNNNNNNGG 5 cut(s) 466, 480, 511, 622, 930
Bse118I RCCGGY 1 cut(s) 755
Bse1I ACTGG 1 cut(s) 877
BseAI TCCGGA 1 cut(s) 459
BseBI CCWGG 3 cut(s) 190, 373, 506
BseDI CCNNGG 2 cut(s) 306, 928
BseGI GGATG 2 cut(s) 520, 871
BseLI CCNNNNNNNGG 5 cut(s) 466, 480, 511, 622, 930
BseMII CTCAG 2 cut(s) 48, 795
BseNI ACTGG 1 cut(s) 877
BseRI GAGGAG 2 cut(s) 482, 920
BseXI GCAGC 2 cut(s) 657, 773
BseYI CCCAGC 1 cut(s) 935
BsgI GTGCAG 1 cut(s) 780
Bsh1236I CGCG 1 cut(s) 122
Bsh1285I CGRYCG 1 cut(s) 382
BshFI GGCC 1 cut(s) 615
BsiEI CGRYCG 1 cut(s) 382
BsiHKAI GWGCWC 2 cut(s) 268, 591
BsiSI CCGG 3 cut(s) 240, 460, 756
BslFI GGGAC 1 cut(s) 57
BslI CCNNNNNNNGG 5 cut(s) 466, 480, 511, 622, 930
BsmAI GTCTC 4 cut(s) 46, 290, 863, 945
BsmBI CGTCTC 1 cut(s) 46
BsmFI GGGAC 1 cut(s) 57
BsmI GAATGC 1 cut(s) 544
BsnI GGCC 1 cut(s) 615
Bso31I GGTCTC 1 cut(s) 945
Bsp1286I GDGCHC 3 cut(s) 268, 474, 591
Bsp13I TCCGGA 1 cut(s) 459
Bsp143I GATC 2 cut(s) 898, 914
BspACI CCGC 3 cut(s) 122, 481, 924
BspANI GGCC 1 cut(s) 615
BspCNI CTCAG 2 cut(s) 47, 794
BspEI TCCGGA 1 cut(s) 459
BspFNI CGCG 1 cut(s) 122
BspLI GGNNCC 1 cut(s) 471
BspMI ACCTGC 1 cut(s) 124
BspPI GGATC 1 cut(s) 922
BspTNI GGTCTC 1 cut(s) 945
BsrFI RCCGGY 1 cut(s) 755
BsrI ACTGG 1 cut(s) 877
BssAI RCCGGY 1 cut(s) 755
BssECI CCNNGG 2 cut(s) 306, 928
BssMI GATC 2 cut(s) 898, 914
BssSI CACGAG 1 cut(s) 902
BssT1I CCWWGG 2 cut(s) 306, 928
Bst2BI CACGAG 1 cut(s) 902
Bst2UI CCWGG 3 cut(s) 190, 373, 506
Bst4CI ACNGT 2 cut(s) 23, 383
Bst6I CTCTTC 1 cut(s) 414
BstC8I GCNNGC 2 cut(s) 675, 726
BstDEI CTNAG 5 cut(s) 34, 315, 621, 781, 996
BstF5I GGATG 2 cut(s) 520, 871
BstFNI CGCG 1 cut(s) 122
BstKTI GATC 2 cut(s) 901, 917
BstMAI GTCTC 4 cut(s) 46, 290, 863, 945
BstMBI GATC 2 cut(s) 898, 914
BstMCI CGRYCG 1 cut(s) 382
BstMWI GCNNNNNNNGC 6 cut(s) 86, 478, 581, 655, 761, 932
BstNI CCWGG 3 cut(s) 190, 373, 506
BstNSI RCATGY 2 cut(s) 732, 808
BstSCI CCNGG 3 cut(s) 188, 371, 504
BstUI CGCG 1 cut(s) 122
BstV1I GCAGC 2 cut(s) 657, 773
BstX2I RGATCY 1 cut(s) 914
BstXI CCANNNNNNTGG 4 cut(s) 208, 260, 350, 935
BstYI RGATCY 1 cut(s) 914
BsuRI GGCC 1 cut(s) 615
BtsCI GGATG 2 cut(s) 520, 871
BtsI GCAGTG 1 cut(s) 75
BtsIMutI CAGTG 1 cut(s) 75
BveI ACCTGC 1 cut(s) 124
Cac8I GCNNGC 2 cut(s) 675, 726
CaiI CAGNNNCTG 1 cut(s) 848
Cfr10I RCCGGY 1 cut(s) 755
CseI GACGC 2 cut(s) 98, 845
CsiI ACCWGGT 1 cut(s) 371
Csp6I GTAC 4 cut(s) 127, 178, 325, 1001
CviAII CATG 4 cut(s) 478, 729, 805, 949
CviQI GTAC 4 cut(s) 127, 178, 325, 1001
DdeI CTNAG 5 cut(s) 34, 315, 621, 781, 996
DpnI GATC 2 cut(s) 900, 916
DpnII GATC 2 cut(s) 898, 914
EaeI YGGCCR 1 cut(s) 613
Eam1104I CTCTTC 1 cut(s) 414
EarI CTCTTC 1 cut(s) 414
EciI GGCGGA 1 cut(s) 913
Ecl136II GAGCTC 1 cut(s) 589
Eco130I CCWWGG 2 cut(s) 306, 928
Eco24I GRGCYC 2 cut(s) 474, 591
Eco31I GGTCTC 1 cut(s) 945
Eco53kI GAGCTC 1 cut(s) 589
EcoICRI GAGCTC 1 cut(s) 589
EcoRII CCWGG 3 cut(s) 188, 371, 504
EcoT14I CCWWGG 2 cut(s) 306, 928
EcoT38I GRGCYC 2 cut(s) 474, 591
ErhI CCWWGG 2 cut(s) 306, 928
Esp3I CGTCTC 1 cut(s) 46
FaeI CATG 4 cut(s) 481, 732, 808, 952
FalI AAGNNNNNCTT 2 cut(s) 167, 199
FaqI GGGAC 1 cut(s) 57
FatI CATG 4 cut(s) 477, 728, 804, 948
FauI CCCGC 1 cut(s) 115
FauNDI CATATG 1 cut(s) 349
FbaI TGATCA 1 cut(s) 898
FblI GTMKAC 1 cut(s) 93
Fnu4HI GCNGC 2 cut(s) 671, 762
FokI GGATG 2 cut(s) 527, 878
FriOI GRGCYC 2 cut(s) 474, 591
Fsp4HI GCNGC 2 cut(s) 671, 762
GluI GCNGC 2 cut(s) 671, 762
GsaI CCCAGC 1 cut(s) 939
GsuI CTGGAG 1 cut(s) 927
HaeIII GGCC 1 cut(s) 615
HapII CCGG 3 cut(s) 240, 460, 756
HgaI GACGC 2 cut(s) 98, 845
Hin1II CATG 4 cut(s) 481, 732, 808, 952
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HindIII AAGCTT 1 cut(s) 683
HinfI GANTC 7 cut(s) 14, 249, 339, 484, 920, 992, 1006
HpaII CCGG 3 cut(s) 240, 460, 756
HphI GGTGA 5 cut(s) 178, 418, 494, 542, 547
Hpy166II GTNNAC 2 cut(s) 94, 695
Hpy188I TCNGA 2 cut(s) 46, 919
Hpy188III TCNNGA 5 cut(s) 460, 783, 848, 902, 966
Hpy8I GTNNAC 2 cut(s) 94, 695
Hpy99I CGWCG 2 cut(s) 95, 98
HpyAV CCTTC 4 cut(s) 20, 396, 563, 780
HpyCH4III ACNGT 2 cut(s) 23, 383
HpyCH4V TGCA 9 cut(s) 115, 218, 398, 542, 605, 677, 724, 761, 804
HpyF10VI GCNNNNNNNGC 6 cut(s) 86, 478, 581, 655, 761, 932
HpyF3I CTNAG 5 cut(s) 34, 315, 621, 781, 996
Hsp92II CATG 4 cut(s) 481, 732, 808, 952
Kpn2I TCCGGA 1 cut(s) 459
Ksp22I TGATCA 1 cut(s) 898
Kzo9I GATC 2 cut(s) 898, 914
LmnI GCTCC 2 cut(s) 469, 850
Lsp1109I GCAGC 2 cut(s) 657, 773
LweI GCATC 1 cut(s) 658
MabI ACCWGGT 1 cut(s) 371
MaeIII GTNAC 3 cut(s) 383, 548, 663
MalI GATC 2 cut(s) 900, 916
MboI GATC 2 cut(s) 898, 914
MboII GAAGA 3 cut(s) 115, 431, 548
MfeI CAATTG 1 cut(s) 393
MflI RGATCY 1 cut(s) 914
MhlI GDGCHC 3 cut(s) 268, 474, 591
MlsI TGGCCA 1 cut(s) 615
MluCI AATT 9 cut(s) 158, 230, 290, 393, 498, 636, 719, 770, 969
MluNI TGGCCA 1 cut(s) 615
MlyI GAGTC 3 cut(s) 348, 914, 1000
MmeI TCCRAC 1 cut(s) 892
Mox20I TGGCCA 1 cut(s) 615
MroI TCCGGA 1 cut(s) 459
MscI TGGCCA 1 cut(s) 615
MseI TTAA 2 cut(s) 531, 639
MslI CAYNNNNRTG 2 cut(s) 206, 708
Msp20I TGGCCA 1 cut(s) 615
MspA1I CMGCKG 1 cut(s) 670
MspI CCGG 3 cut(s) 240, 460, 756
MspR9I CCNGG 3 cut(s) 190, 373, 506
MunI CAATTG 1 cut(s) 393
Mva1269I GAATGC 1 cut(s) 544
MvaI CCWGG 3 cut(s) 190, 373, 506
MvnI CGCG 1 cut(s) 122
MwoI GCNNNNNNNGC 6 cut(s) 86, 478, 581, 655, 761, 932
NdeI CATATG 1 cut(s) 349
NdeII GATC 2 cut(s) 898, 914
NlaIII CATG 4 cut(s) 481, 732, 808, 952
NlaIV GGNNCC 1 cut(s) 471
NmuCI GTSAC 2 cut(s) 383, 548
NspI RCATGY 2 cut(s) 732, 808
OliI CACNNNNGTG 1 cut(s) 206
PcsI WCGNNNNNNNCGW 2 cut(s) 93, 126
PctI GAATGC 1 cut(s) 544
PfeI GAWTC 4 cut(s) 14, 249, 484, 992
PkrI GCNGC 2 cut(s) 672, 763
PleI GAGTC 3 cut(s) 347, 914, 1000
PpsI GAGTC 3 cut(s) 347, 914, 1000
Psp124BI GAGCTC 1 cut(s) 591
Psp6I CCWGG 3 cut(s) 188, 371, 504
PspFI CCCAGC 1 cut(s) 935
PspGI CCWGG 3 cut(s) 188, 371, 504
PspN4I GGNNCC 1 cut(s) 471
PstNI CAGNNNCTG 1 cut(s) 848
PsuI RGATCY 1 cut(s) 914
PvuII CAGCTG 1 cut(s) 670
RsaI GTAC 4 cut(s) 128, 179, 326, 1002
RsaNI GTAC 4 cut(s) 127, 178, 325, 1001
RseI CAYNNNNRTG 2 cut(s) 206, 708
SacI GAGCTC 1 cut(s) 591
SalI GTCGAC 1 cut(s) 92
SaqAI TTAA 2 cut(s) 531, 639
SatI GCNGC 2 cut(s) 671, 762
Sau3AI GATC 2 cut(s) 898, 914
ScaI AGTACT 1 cut(s) 179
SchI GAGTC 3 cut(s) 348, 914, 1000
ScrFI CCNGG 3 cut(s) 190, 373, 506
SduI GDGCHC 3 cut(s) 268, 474, 591
SexAI ACCWGGT 1 cut(s) 371
SfaNI GCATC 1 cut(s) 658
SgrDI CGTCGACG 1 cut(s) 92
SmiMI CAYNNNNRTG 2 cut(s) 206, 708
SmlI CTYRAG 1 cut(s) 860
SmoI CTYRAG 1 cut(s) 860
Sse9I AATT 9 cut(s) 158, 230, 290, 393, 498, 636, 719, 770, 969
SsiI CCGC 3 cut(s) 122, 481, 924
SstI GAGCTC 1 cut(s) 591
StyD4I CCNGG 3 cut(s) 188, 371, 504
StyI CCWWGG 2 cut(s) 306, 928
TaaI ACNGT 2 cut(s) 23, 383
TaqI TCGA 2 cut(s) 93, 378
TasI AATT 9 cut(s) 158, 230, 290, 393, 498, 636, 719, 770, 969
TatI WGTACW 1 cut(s) 177
TfiI GAWTC 4 cut(s) 14, 249, 484, 992
Tru1I TTAA 2 cut(s) 531, 639
Tru9I TTAA 2 cut(s) 531, 639
TscAI CASTG 1 cut(s) 82
TseFI GTSAC 2 cut(s) 383, 548
TseI GCWGC 2 cut(s) 670, 761
Tsp45I GTSAC 2 cut(s) 383, 548
TspRI CASTG 1 cut(s) 82
XapI RAATTY 2 cut(s) 230, 770
XceI RCATGY 2 cut(s) 732, 808
XmiI GTMKAC 1 cut(s) 93
ZrmI AGTACT 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.