MD06G1148000.v1.1

Belongs to the sulfotransferase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0019270
Physical Location & Seq
Reverse (-)
29031587 .. 29032424
838 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1148000.v1.1.491

Sequence Viewer

Length: 777 bp
ATGTTTGCTACACTCAACAGAAGCCTCTATGATGCTGCCTCTCCTCATCATCCCTTGCTCACAACTGGGCCCCACGACTGTTTTCCTTTCTTGGATATGGATGACATGCTTGGAAACCATGATCACAACCGTCCAGTAACCTCACTTGATCATGATCACGACGATCGTGACGGTGGTGATGGTATGATTAATAATTATGTTCCGCCTACAAGCACTACTCAACTCTTTGCGACCCACCTGCCTTACTCTTTGTTACCAAAATCTGTGACTACTTCTAGTAGTACTTGCAGTGTGTCAAAATTCGTATACGTTTACCGTAACCCTAAAGATGTATTCATTTCTTCATGGATCTTCGCGAGCAAAGTTAGAGCAATAAACACAAGACTTGCACCCTTCTCACTAGAAGAAGCATTTGAGATTTTCTGCCGCGGAGTCTGCCCATACGGGCCCTATTGGGACCATGTGCTAGGGTTTTGGAAAGCAAGCTTGGAAATGCCAGAGCAAGTTTGGTTCTTAAAATATGAGGATCTCAAGAAGGAGCCATCTGCTAATGTGAAAAGACTGGCTGAGTTCTTGGGTCAACCTTTTTCAGAGGAGGAGGAGAGCAAAGGGGTGGTGCAACAGATCATAAAGCTTTGTAGTTTTGAGAATCTGAGCAGCTTGGAGGTAAATAAAACCTCCAGGACGCAACAGTACTTTGTTAAAGCCAATATTGTCTTCGAGAACAGTGACTTCTTCAGGAAAGGCCAAATGAGAGGTTCAGTGGTTCTGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sulfotransfer_1

Protein Analysis

259

Amino Acids

29.44

Weight (kDa)

5.71

Isoelectric Point (pI)

37.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sulfotransfer_1 PF00685 13 - 251 1.6e-45 Sulfotransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 246
Acc36I ACCTGC 1 cut(s) 246
AccI GTMKAC 1 cut(s) 306
AccII CGCG 2 cut(s) 356, 429
AciI CCGC 3 cut(s) 203, 427, 429
AclWI GGATC 2 cut(s) 356, 534
AcsI RAATTY 1 cut(s) 299
AcuI CTGAAG 1 cut(s) 721
AfaI GTAC 2 cut(s) 283, 695
AjnI CCWGG 1 cut(s) 680
AjuI GAANNNNNNNTTGG 4 cut(s) 470, 502, 701, 733
AluBI AGCT 3 cut(s) 486, 634, 660
AluI AGCT 3 cut(s) 486, 634, 660
AlwI GGATC 2 cut(s) 356, 534
AoxI GGCC 3 cut(s) 68, 446, 745
ApaI GGGCCC 2 cut(s) 72, 450
ApeKI GCWGC 2 cut(s) 35, 657
ApoI RAATTY 1 cut(s) 299
AseI ATTAAT 1 cut(s) 189
AspS9I GGNCC 5 cut(s) 68, 69, 446, 447, 457
AsuHPI GGTGA 1 cut(s) 188
AvaII GGWCC 1 cut(s) 457
BaeGI GKGCMC 2 cut(s) 72, 450
BanII GRGCYC 2 cut(s) 72, 450
BbsI GAAGAC 1 cut(s) 709
BbvI GCAGC 2 cut(s) 22, 669
BccI CCATC 2 cut(s) 173, 550
BcgI CGANNNNNNTGC 2 cut(s) 220, 254
BciT130I CCWGG 1 cut(s) 682
BclI TGATCA 3 cut(s) 121, 148, 154
BfaI CTAG 3 cut(s) 276, 401, 467
BfuAI ACCTGC 1 cut(s) 246
BisI GCNGC 3 cut(s) 36, 427, 658
BlsI GCNGC 3 cut(s) 37, 428, 659
BmcAI AGTACT 2 cut(s) 283, 695
Bme1390I CCNGG 1 cut(s) 682
Bme18I GGWCC 1 cut(s) 457
BmgT120I GGNCC 5 cut(s) 68, 69, 446, 447, 457
BmiI GGNNCC 5 cut(s) 70, 71, 448, 458, 540
BmrFI CCNGG 1 cut(s) 682
BmrI ACTGGG 1 cut(s) 75
BmsI GCATC 1 cut(s) 22
BmuI ACTGGG 1 cut(s) 75
BpiI GAAGAC 1 cut(s) 709
BpmI CTGGAG 1 cut(s) 664
BpuEI CTTGAG 1 cut(s) 515
BsaBI GATNNNNATC 1 cut(s) 153
BsaJI CCNNGG 1 cut(s) 427
Bse1I ACTGG 3 cut(s) 70, 134, 567
Bse8I GATNNNNATC 1 cut(s) 153
BseBI CCWGG 1 cut(s) 682
BseDI CCNNGG 1 cut(s) 427
BseGI GGATG 2 cut(s) 49, 106
BseJI GATNNNNATC 1 cut(s) 153
BseMII CTCAG 2 cut(s) 558, 644
BseNI ACTGG 3 cut(s) 70, 134, 567
BseRI GAGGAG 4 cut(s) 33, 608, 611, 614
BseSI GKGCMC 2 cut(s) 72, 450
BseXI GCAGC 2 cut(s) 22, 669
Bsh1236I CGCG 2 cut(s) 356, 429
Bsh1285I CGRYCG 1 cut(s) 166
BshFI GGCC 3 cut(s) 70, 448, 747
BsiEI CGRYCG 1 cut(s) 166
BslFI GGGAC 1 cut(s) 470
BsmFI GGGAC 1 cut(s) 470
BsnI GGCC 3 cut(s) 70, 448, 747
Bsp120I GGGCCC 2 cut(s) 68, 446
Bsp1286I GDGCHC 2 cut(s) 72, 450
Bsp143I GATC 7 cut(s) 121, 148, 154, 163, 348, 526, 624
Bsp68I TCGCGA 1 cut(s) 356
BspACI CCGC 3 cut(s) 203, 427, 429
BspANI GGCC 3 cut(s) 70, 448, 747
BspCNI CTCAG 2 cut(s) 559, 645
BspFNI CGCG 2 cut(s) 356, 429
BspHI TCATGA 1 cut(s) 151
BspLI GGNNCC 5 cut(s) 70, 71, 448, 458, 540
BspMI ACCTGC 1 cut(s) 246
BspPI GGATC 2 cut(s) 356, 534
BsrI ACTGG 3 cut(s) 70, 134, 567
BssECI CCNNGG 1 cut(s) 427
BssMI GATC 7 cut(s) 121, 148, 154, 163, 348, 526, 624
BssNAI GTATAC 1 cut(s) 307
Bst1107I GTATAC 1 cut(s) 307
Bst2UI CCWGG 1 cut(s) 682
Bst4CI ACNGT 6 cut(s) 80, 131, 173, 317, 693, 728
BstC8I GCNNGC 2 cut(s) 358, 484
BstDEI CTNAG 2 cut(s) 567, 653
BstDSI CCRYGG 1 cut(s) 427
BstF5I GGATG 2 cut(s) 49, 106
BstFNI CGCG 2 cut(s) 356, 429
BstKTI GATC 7 cut(s) 124, 151, 157, 166, 351, 529, 627
BstMBI GATC 7 cut(s) 121, 148, 154, 163, 348, 526, 624
BstMCI CGRYCG 1 cut(s) 166
BstMWI GCNNNNNNNGC 1 cut(s) 435
BstNI CCWGG 1 cut(s) 682
BstNSI RCATGY 1 cut(s) 109
BstSCI CCNGG 1 cut(s) 680
BstSLI GKGCMC 2 cut(s) 72, 450
BstUI CGCG 2 cut(s) 356, 429
BstV1I GCAGC 2 cut(s) 22, 669
BstV2I GAAGAC 1 cut(s) 709
BstX2I RGATCY 2 cut(s) 348, 526
BstYI RGATCY 2 cut(s) 348, 526
BstZ17I GTATAC 1 cut(s) 307
BsuRI GGCC 3 cut(s) 70, 448, 747
BtgI CCRYGG 1 cut(s) 427
BtsCI GGATG 2 cut(s) 49, 106
BtsI GCAGTG 1 cut(s) 295
BtsIMutI CAGTG 3 cut(s) 295, 733, 768
BtuMI TCGCGA 1 cut(s) 356
BveI ACCTGC 1 cut(s) 246
Cac8I GCNNGC 2 cut(s) 358, 484
CciI TCATGA 1 cut(s) 151
Cfr13I GGNCC 5 cut(s) 68, 69, 446, 447, 457
Cfr42I CCGCGG 1 cut(s) 430
CseI GACGC 1 cut(s) 694
Csp6I GTAC 2 cut(s) 282, 694
CviAII CATG 5 cut(s) 106, 119, 152, 345, 461
CviQI GTAC 2 cut(s) 282, 694
DdeI CTNAG 2 cut(s) 567, 653
DpnI GATC 7 cut(s) 123, 150, 156, 165, 350, 528, 626
DpnII GATC 7 cut(s) 121, 148, 154, 163, 348, 526, 624
EciI GGCGGA 1 cut(s) 192
Eco24I GRGCYC 2 cut(s) 72, 450
Eco47I GGWCC 1 cut(s) 457
Eco57I CTGAAG 1 cut(s) 721
EcoO109I RGGNCCY 2 cut(s) 69, 447
EcoRII CCWGG 1 cut(s) 680
EcoT38I GRGCYC 2 cut(s) 72, 450
FaeI CATG 5 cut(s) 109, 122, 155, 348, 464
FaqI GGGAC 1 cut(s) 470
FatI CATG 5 cut(s) 105, 118, 151, 344, 460
FbaI TGATCA 3 cut(s) 121, 148, 154
FblI GTMKAC 1 cut(s) 306
Fnu4HI GCNGC 3 cut(s) 36, 427, 658
FokI GGATG 2 cut(s) 36, 113
FriOI GRGCYC 2 cut(s) 72, 450
Fsp4HI GCNGC 3 cut(s) 36, 427, 658
FspBI CTAG 3 cut(s) 276, 401, 467
GluI GCNGC 3 cut(s) 36, 427, 658
GsuI CTGGAG 1 cut(s) 664
HaeIII GGCC 3 cut(s) 70, 448, 747
HgaI GACGC 1 cut(s) 694
Hin1II CATG 5 cut(s) 109, 122, 155, 348, 464
HincII GTYRAC 1 cut(s) 581
HindII GTYRAC 1 cut(s) 581
HindIII AAGCTT 2 cut(s) 484, 632
HinfI GANTC 2 cut(s) 432, 649
HphI GGTGA 1 cut(s) 188
Hpy166II GTNNAC 3 cut(s) 307, 313, 581
Hpy188I TCNGA 2 cut(s) 592, 654
Hpy188III TCNNGA 8 cut(s) 152, 158, 167, 355, 532, 721, 739, 770
Hpy8I GTNNAC 3 cut(s) 307, 313, 581
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 2 cut(s) 403, 529
HpyCH4III ACNGT 6 cut(s) 80, 131, 173, 317, 693, 728
HpyCH4IV ACGT 1 cut(s) 309
HpyCH4V TGCA 3 cut(s) 288, 389, 619
HpyF10VI GCNNNNNNNGC 1 cut(s) 435
HpyF3I CTNAG 2 cut(s) 567, 653
HpySE526I ACGT 1 cut(s) 309
Hsp92II CATG 5 cut(s) 109, 122, 155, 348, 464
Ksp22I TGATCA 3 cut(s) 121, 148, 154
KspI CCGCGG 1 cut(s) 430
Kzo9I GATC 7 cut(s) 121, 148, 154, 163, 348, 526, 624
LmnI GCTCC 1 cut(s) 538
LpnPI CCDG 9 cut(s) 51, 147, 251, 510, 548, 667, 694, 724, 755
Lsp1109I GCAGC 2 cut(s) 22, 669
LweI GCATC 1 cut(s) 22
MaeI CTAG 3 cut(s) 276, 401, 467
MaeII ACGT 1 cut(s) 309
MaeIII GTNAC 6 cut(s) 136, 167, 252, 265, 317, 728
MalI GATC 7 cut(s) 123, 150, 156, 165, 350, 528, 626
MboI GATC 7 cut(s) 121, 148, 154, 163, 348, 526, 624
MboII GAAGA 5 cut(s) 333, 343, 416, 709, 727
MflI RGATCY 2 cut(s) 348, 526
MhlI GDGCHC 2 cut(s) 72, 450
MluCI AATT 2 cut(s) 193, 299
MlyI GAGTC 1 cut(s) 441
MseI TTAA 3 cut(s) 189, 515, 702
MspA1I CMGCKG 1 cut(s) 429
MspR9I CCNGG 1 cut(s) 682
MvaI CCWGG 1 cut(s) 682
MvnI CGCG 2 cut(s) 356, 429
MwoI GCNNNNNNNGC 1 cut(s) 435
NdeII GATC 7 cut(s) 121, 148, 154, 163, 348, 526, 624
NlaIII CATG 5 cut(s) 109, 122, 155, 348, 464
NlaIV GGNNCC 5 cut(s) 70, 71, 448, 458, 540
NmuCI GTSAC 3 cut(s) 167, 265, 728
NruI TCGCGA 1 cut(s) 356
NspI RCATGY 1 cut(s) 109
PagI TCATGA 1 cut(s) 151
PaqCI CACCTGC 1 cut(s) 246
PfeI GAWTC 1 cut(s) 649
PfoI TCCNGGA 1 cut(s) 680
PkrI GCNGC 3 cut(s) 37, 428, 659
Ple19I CGATCG 1 cut(s) 166
PleI GAGTC 1 cut(s) 440
PpsI GAGTC 1 cut(s) 440
PshBI ATTAAT 1 cut(s) 189
Psp6I CCWGG 1 cut(s) 680
PspGI CCWGG 1 cut(s) 680
PspN4I GGNNCC 5 cut(s) 70, 71, 448, 458, 540
PspOMI GGGCCC 2 cut(s) 68, 446
PspPI GGNCC 5 cut(s) 68, 69, 446, 447, 457
PsuI RGATCY 2 cut(s) 348, 526
PvuI CGATCG 1 cut(s) 166
RruI TCGCGA 1 cut(s) 356
RsaI GTAC 2 cut(s) 283, 695
RsaNI GTAC 2 cut(s) 282, 694
SacII CCGCGG 1 cut(s) 430
SaqAI TTAA 3 cut(s) 189, 515, 702
SatI GCNGC 3 cut(s) 36, 427, 658
Sau3AI GATC 7 cut(s) 121, 148, 154, 163, 348, 526, 624
Sau96I GGNCC 5 cut(s) 68, 69, 446, 447, 457
ScaI AGTACT 2 cut(s) 283, 695
SchI GAGTC 1 cut(s) 441
ScrFI CCNGG 1 cut(s) 682
SduI GDGCHC 2 cut(s) 72, 450
SfaNI GCATC 1 cut(s) 22
Sfr303I CCGCGG 1 cut(s) 430
SgrBI CCGCGG 1 cut(s) 430
SinI GGWCC 1 cut(s) 457
SmlI CTYRAG 1 cut(s) 530
SmoI CTYRAG 1 cut(s) 530
Sse9I AATT 2 cut(s) 193, 299
SsiI CCGC 3 cut(s) 203, 427, 429
SspI AATATT 1 cut(s) 712
SspMI CTAG 3 cut(s) 276, 401, 467
StyD4I CCNGG 1 cut(s) 680
TaaI ACNGT 6 cut(s) 80, 131, 173, 317, 693, 728
TaiI ACGT 1 cut(s) 312
TaqI TCGA 1 cut(s) 720
TasI AATT 2 cut(s) 193, 299
TatI WGTACW 2 cut(s) 281, 693
TauI GCSGC 1 cut(s) 429
TfiI GAWTC 1 cut(s) 649
Tru1I TTAA 3 cut(s) 189, 515, 702
Tru9I TTAA 3 cut(s) 189, 515, 702
TscAI CASTG 3 cut(s) 295, 733, 768
TseFI GTSAC 3 cut(s) 167, 265, 728
TseI GCWGC 2 cut(s) 35, 657
Tsp45I GTSAC 3 cut(s) 167, 265, 728
TspDTI ATGAA 2 cut(s) 325, 333
TspRI CASTG 3 cut(s) 295, 733, 768
VpaK11BI GGWCC 1 cut(s) 457
VspI ATTAAT 1 cut(s) 189
XapI RAATTY 1 cut(s) 299
XceI RCATGY 1 cut(s) 109
XcmI CCANNNNNNNNNTGG 1 cut(s) 504
XmiI GTMKAC 1 cut(s) 306
XspI CTAG 3 cut(s) 276, 401, 467
ZrmI AGTACT 2 cut(s) 283, 695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.