MD06G1149300.v1.1

Belongs to the sulfotransferase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0019270
Physical Location & Seq
Forward (+)
29185114 .. 29185644
531 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1149300.v1.1.491

Sequence Viewer

Length: 531 bp
ATGTCTTCCGCTTCGACGTCCCGACCTTTTGTTCGTAAAACCAATTATATTGTGGAGAAGGAAAAAGGGGAAGCCTACCGGAAAATCGTTGCGGAAATTTGTAGAGAAATGCTGCCAAATCCTCCGAAAGAGAAGGGTTGGACCTCTAAACACTTGGTTCAGTACCAAGGCTTCTGGATAAACCCTACCTTTATGTTAAAAGCAGCCCAACTTCAACAACATTTCAACTTATTCCAAACAACCGGAAGTTCTACCAGCGCCGCCCGTGGGTCGTCCCCCATATTCTTCTTGGCCACATTTTCGAAATCAGGTACGACGTGGCTTAGAGCACTCATGTTCGCTGTACTCAACAAAAGCCTCTATGATGTTGCCTCTCCTCATCATCCCTTACTCACAACTGGGCCCCACGACTGCTTTCCTCTCTTGGTGTTGGATAACATGCTTGGAAATCATGATCACAACCGTCCCGTAACCTCACTTGATCATGATCACGACGATCGTGACGGTGGTGATGGTATGATTGATAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sulfotransfer_1

Protein Analysis

177

Amino Acids

19.79

Weight (kDa)

7.87

Isoelectric Point (pI)

30.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 20
AciI CCGC 3 cut(s) 9, 92, 261
AcoI YGGCCR 1 cut(s) 291
AcsI RAATTY 1 cut(s) 96
AcyI GRCGYC 1 cut(s) 17
AfaI GTAC 3 cut(s) 164, 313, 345
AfiI CCNNNNNNNGG 1 cut(s) 267
AgsI TTSAA 2 cut(s) 215, 226
AjiI CACGTC 1 cut(s) 318
Alw21I GWGCWC 1 cut(s) 331
AoxI GGCC 2 cut(s) 291, 401
ApaI GGGCCC 1 cut(s) 405
ApeKI GCWGC 2 cut(s) 112, 203
ApoI RAATTY 1 cut(s) 96
ArsI GACNNNNNNTTYG 2 cut(s) 15, 47
AspLEI GCGC 1 cut(s) 260
AspS9I GGNCC 3 cut(s) 141, 401, 402
AsuHPI GGTGA 1 cut(s) 521
AsuII TTCGAA 1 cut(s) 302
AvaII GGWCC 1 cut(s) 141
BaeGI GKGCMC 1 cut(s) 405
BalI TGGCCA 1 cut(s) 293
BanII GRGCYC 1 cut(s) 405
BarI GAAGNNNNNNTAC 2 cut(s) 155, 187
Bbv12I GWGCWC 1 cut(s) 331
BbvI GCAGC 2 cut(s) 99, 215
BccI CCATC 1 cut(s) 506
BclI TGATCA 3 cut(s) 454, 481, 487
BfoI RGCGCY 1 cut(s) 261
BisI GCNGC 3 cut(s) 113, 204, 261
BlsI GCNGC 3 cut(s) 114, 205, 262
Bme18I GGWCC 1 cut(s) 141
BmgBI CACGTC 1 cut(s) 318
BmgT120I GGNCC 3 cut(s) 141, 401, 402
BmiI GGNNCC 2 cut(s) 403, 404
BmrI ACTGGG 1 cut(s) 408
BmuI ACTGGG 1 cut(s) 408
Bpu14I TTCGAA 1 cut(s) 302
BsaBI GATNNNNATC 1 cut(s) 486
BsaHI GRCGYC 1 cut(s) 17
BsaJI CCNNGG 2 cut(s) 166, 265
BsaWI WCCGGW 2 cut(s) 78, 242
Bsc4I CCNNNNNNNGG 1 cut(s) 267
Bse1I ACTGG 1 cut(s) 403
Bse8I GATNNNNATC 1 cut(s) 486
BseDI CCNNGG 2 cut(s) 166, 265
BseGI GGATG 1 cut(s) 382
BseJI GATNNNNATC 1 cut(s) 486
BseLI CCNNNNNNNGG 1 cut(s) 267
BseNI ACTGG 1 cut(s) 403
BseRI GAGGAG 1 cut(s) 366
BseSI GKGCMC 1 cut(s) 405
BseXI GCAGC 2 cut(s) 99, 215
Bsh1285I CGRYCG 1 cut(s) 499
BshFI GGCC 2 cut(s) 293, 403
BsiEI CGRYCG 1 cut(s) 499
BsiHKAI GWGCWC 1 cut(s) 331
BsiSI CCGG 2 cut(s) 79, 243
BslFI GGGAC 3 cut(s) 4, 259, 450
BslI CCNNNNNNNGG 1 cut(s) 267
BsmFI GGGAC 3 cut(s) 4, 259, 450
BsnI GGCC 2 cut(s) 293, 403
Bsp119I TTCGAA 1 cut(s) 302
Bsp120I GGGCCC 1 cut(s) 401
Bsp1286I GDGCHC 2 cut(s) 331, 405
Bsp143I GATC 4 cut(s) 454, 481, 487, 496
BspACI CCGC 3 cut(s) 9, 92, 261
BspANI GGCC 2 cut(s) 293, 403
BspHI TCATGA 2 cut(s) 451, 484
BspLI GGNNCC 2 cut(s) 403, 404
BspT104I TTCGAA 1 cut(s) 302
BsrI ACTGG 1 cut(s) 403
BssECI CCNNGG 2 cut(s) 166, 265
BssMI GATC 4 cut(s) 454, 481, 487, 496
BssNI GRCGYC 1 cut(s) 17
BssT1I CCWWGG 1 cut(s) 166
Bst4CI ACNGT 2 cut(s) 464, 506
BstACI GRCGYC 1 cut(s) 17
BstBI TTCGAA 1 cut(s) 302
BstDEI CTNAG 1 cut(s) 323
BstDSI CCRYGG 1 cut(s) 265
BstF5I GGATG 1 cut(s) 382
BstH2I RGCGCY 1 cut(s) 261
BstHHI GCGC 1 cut(s) 260
BstKTI GATC 4 cut(s) 457, 484, 490, 499
BstMBI GATC 4 cut(s) 454, 481, 487, 496
BstMCI CGRYCG 1 cut(s) 499
BstNSI RCATGY 1 cut(s) 442
BstSLI GKGCMC 1 cut(s) 405
BstV1I GCAGC 2 cut(s) 99, 215
BsuRI GGCC 2 cut(s) 293, 403
BtgI CCRYGG 1 cut(s) 265
BtrI CACGTC 1 cut(s) 318
BtsCI GGATG 1 cut(s) 382
CciI TCATGA 2 cut(s) 451, 484
CfoI GCGC 1 cut(s) 260
Cfr13I GGNCC 3 cut(s) 141, 401, 402
Csp6I GTAC 3 cut(s) 163, 312, 344
CviAII CATG 4 cut(s) 334, 439, 452, 485
CviJI RGCY 7 cut(s) 74, 171, 206, 293, 322, 357, 403
CviKI_1 RGCY 7 cut(s) 74, 171, 206, 293, 322, 357, 403
CviQI GTAC 3 cut(s) 163, 312, 344
DdeI CTNAG 1 cut(s) 323
DpnI GATC 4 cut(s) 456, 483, 489, 498
DpnII GATC 4 cut(s) 454, 481, 487, 496
EaeI YGGCCR 1 cut(s) 291
Eco130I CCWWGG 1 cut(s) 166
Eco24I GRGCYC 1 cut(s) 405
Eco47I GGWCC 1 cut(s) 141
EcoO109I RGGNCCY 1 cut(s) 402
EcoT14I CCWWGG 1 cut(s) 166
EcoT38I GRGCYC 1 cut(s) 405
ErhI CCWWGG 1 cut(s) 166
FaeI CATG 4 cut(s) 337, 442, 455, 488
FaiI YATR 9 cut(s) 48, 194, 281, 335, 363, 440, 453, 486, 518
FaqI GGGAC 3 cut(s) 4, 259, 450
FatI CATG 4 cut(s) 333, 438, 451, 484
FbaI TGATCA 3 cut(s) 454, 481, 487
Fnu4HI GCNGC 3 cut(s) 113, 204, 261
FokI GGATG 1 cut(s) 369
FriOI GRGCYC 1 cut(s) 405
Fsp4HI GCNGC 3 cut(s) 113, 204, 261
GlaI GCGC 1 cut(s) 259
GluI GCNGC 3 cut(s) 113, 204, 261
HaeII RGCGCY 1 cut(s) 261
HaeIII GGCC 2 cut(s) 293, 403
HapII CCGG 2 cut(s) 79, 243
HhaI GCGC 1 cut(s) 260
Hin1I GRCGYC 1 cut(s) 17
Hin1II CATG 4 cut(s) 337, 442, 455, 488
Hin6I GCGC 1 cut(s) 258
HinP1I GCGC 1 cut(s) 258
HpaII CCGG 2 cut(s) 79, 243
HphI GGTGA 1 cut(s) 521
Hpy188I TCNGA 1 cut(s) 126
Hpy188III TCNNGA 6 cut(s) 21, 175, 452, 485, 491, 500
Hpy99I CGWCG 3 cut(s) 19, 319, 497
HpyAV CCTTC 2 cut(s) 52, 127
HpyCH4III ACNGT 2 cut(s) 464, 506
HpyCH4IV ACGT 2 cut(s) 17, 317
HpyF3I CTNAG 1 cut(s) 323
HpySE526I ACGT 2 cut(s) 17, 317
Hsp92I GRCGYC 1 cut(s) 17
Hsp92II CATG 4 cut(s) 337, 442, 455, 488
HspAI GCGC 1 cut(s) 258
Ksp22I TGATCA 3 cut(s) 454, 481, 487
Kzo9I GATC 4 cut(s) 454, 481, 487, 496
LpnPI CCDG 6 cut(s) 92, 160, 256, 268, 294, 384
Lsp1109I GCAGC 2 cut(s) 99, 215
MaeII ACGT 2 cut(s) 17, 317
MaeIII GTNAC 2 cut(s) 469, 500
MalI GATC 4 cut(s) 456, 483, 489, 498
MboI GATC 4 cut(s) 454, 481, 487, 496
MboII GAAGA 1 cut(s) 277
MhlI GDGCHC 2 cut(s) 331, 405
MlsI TGGCCA 1 cut(s) 293
MluCI AATT 3 cut(s) 43, 96, 526
MluNI TGGCCA 1 cut(s) 293
MmeI TCCRAC 2 cut(s) 119, 411
MnlI CCTC 7 cut(s) 132, 154, 368, 382, 387, 429, 484
Mox20I TGGCCA 1 cut(s) 293
MscI TGGCCA 1 cut(s) 293
MseI TTAA 2 cut(s) 197, 529
Msp20I TGGCCA 1 cut(s) 293
MspI CCGG 2 cut(s) 79, 243
NdeII GATC 4 cut(s) 454, 481, 487, 496
NlaIII CATG 4 cut(s) 337, 442, 455, 488
NlaIV GGNNCC 2 cut(s) 403, 404
NmuCI GTSAC 1 cut(s) 500
NspI RCATGY 1 cut(s) 442
NspV TTCGAA 1 cut(s) 302
PagI TCATGA 2 cut(s) 451, 484
PkrI GCNGC 3 cut(s) 114, 205, 262
Ple19I CGATCG 1 cut(s) 499
PspN4I GGNNCC 2 cut(s) 403, 404
PspOMI GGGCCC 1 cut(s) 401
PspPI GGNCC 3 cut(s) 141, 401, 402
PvuI CGATCG 1 cut(s) 499
RsaI GTAC 3 cut(s) 164, 313, 345
RsaNI GTAC 3 cut(s) 163, 312, 344
SaqAI TTAA 2 cut(s) 197, 529
SatI GCNGC 3 cut(s) 113, 204, 261
Sau3AI GATC 4 cut(s) 454, 481, 487, 496
Sau96I GGNCC 3 cut(s) 141, 401, 402
SduI GDGCHC 2 cut(s) 331, 405
SetI ASST 7 cut(s) 20, 28, 146, 191, 313, 320, 476
SfuI TTCGAA 1 cut(s) 302
SinI GGWCC 1 cut(s) 141
Sse9I AATT 3 cut(s) 43, 96, 526
SsiI CCGC 3 cut(s) 9, 92, 261
StyI CCWWGG 1 cut(s) 166
TaaI ACNGT 2 cut(s) 464, 506
TaiI ACGT 2 cut(s) 20, 320
TaqI TCGA 2 cut(s) 14, 302
TasI AATT 3 cut(s) 43, 96, 526
TatI WGTACW 1 cut(s) 343
TauI GCSGC 1 cut(s) 263
Tru1I TTAA 2 cut(s) 197, 529
Tru9I TTAA 2 cut(s) 197, 529
TseFI GTSAC 1 cut(s) 500
TseI GCWGC 2 cut(s) 112, 203
Tsp45I GTSAC 1 cut(s) 500
VpaK11BI GGWCC 1 cut(s) 141
XapI RAATTY 1 cut(s) 96
XceI RCATGY 1 cut(s) 442
XcmI CCANNNNNNNNNTGG 2 cut(s) 49, 286
ZraI GACGTC 1 cut(s) 18
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.