MD06G1149500.v1.1

Belongs to the sulfotransferase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0019270
Physical Location & Seq
Forward (+)
29206532 .. 29207290
759 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1149500.v1.1.491

Sequence Viewer

Length: 759 bp
ATGGATAACATGCTTGGAAACCATGATCACAGTCGTCCCGTAACCTCACTTGATCATGATCACGACGATCGTGACGGTGGTGATGGTATGATTAATAATTATGTTCCGCCTACAAGCACTACACAACTCTTTGCGACCCACCTGCCTTACTCTTTGTTACCAAAGTCTGTGACTACTTCTAGTAGTGCTTATTGTGTGTCAAAATTCGTTTACGTTTGCCGCAACCCTAAAGATGGATTCATTTCTTTATGGAAGTTCGGGAACAAAGTTAAACCAGTAAACACAGGACTTGCACCCTTATCAATAGAAGAAGCATTTGAGCTTTTCTGCCGCGGAGTCTACCCGTACGGGCCCTATTGGGACCATGTGCTAGGGTTTTGGAAAGCAAGCTTGGAAATGCCAGAGAAAGTTTTGTTCTTAAAATATGAGGATCTCAGGAAGGAGCCATCTGCTAATGTGAAGAGACTGGCTGAGTTCTTGGGTCAACCTTTCTCAGAGGAGGAGGAGAGCAAAGGGGTGGTGCAGCAGATCATAAAGCTTTGTAGTTTTGAGAATCTGAGCAGCTTGGAGGTAAATAAAACCTCCAGGACGCAACAGTACCTTGTTAAAGCCAATATCGTCTTCGAGAACAGTGACTTCTTCAGGAAAGGTCAAGTTGGGGATTGGAAGAACTTCTTCACTGATGATATGGCAAAACGTATGGATCAGATCATAGATGAGAGGTTCAGTGGTTCTGGATTGACATTTGCTAATCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sulfotransfer_1

Protein Analysis

253

Amino Acids

28.71

Weight (kDa)

5.72

Isoelectric Point (pI)

35.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sulfotransfer_1 PF00685 40 - 246 2.9e-55 Sulfotransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 150
Acc36I ACCTGC 1 cut(s) 150
AccI GTMKAC 1 cut(s) 339
AccII CGCG 1 cut(s) 333
AciI CCGC 4 cut(s) 107, 220, 331, 333
AclWI GGATC 2 cut(s) 438, 711
AcsI RAATTY 1 cut(s) 203
AcuI CTGAAG 1 cut(s) 625
AfaI GTAC 2 cut(s) 347, 599
AfiI CCNNNNNNNGG 1 cut(s) 233
AjnI CCWGG 1 cut(s) 584
AjuI GAANNNNNNNTTGG 4 cut(s) 374, 406, 605, 637
AluBI AGCT 4 cut(s) 322, 390, 538, 564
AluI AGCT 4 cut(s) 322, 390, 538, 564
Alw26I GTCTC 1 cut(s) 457
AlwI GGATC 2 cut(s) 438, 711
AoxI GGCC 1 cut(s) 350
ApaI GGGCCC 1 cut(s) 354
ApeKI GCWGC 2 cut(s) 523, 561
ApoI RAATTY 1 cut(s) 203
AseI ATTAAT 1 cut(s) 93
Asp700I GAANNNNTTC 2 cut(s) 671, 674
AspS9I GGNCC 3 cut(s) 350, 351, 361
AsuHPI GGTGA 1 cut(s) 92
AvaII GGWCC 1 cut(s) 361
BaeGI GKGCMC 1 cut(s) 354
BanII GRGCYC 1 cut(s) 354
BbsI GAAGAC 1 cut(s) 613
BbvI GCAGC 2 cut(s) 535, 573
BccI CCATC 3 cut(s) 77, 227, 454
BcgI CGANNNNNNTGC 2 cut(s) 124, 158
BciT130I CCWGG 1 cut(s) 586
BclI TGATCA 3 cut(s) 25, 52, 58
BcoDI GTCTC 1 cut(s) 457
BfaI CTAG 2 cut(s) 180, 371
BfuAI ACCTGC 1 cut(s) 150
BisI GCNGC 4 cut(s) 220, 331, 524, 562
BlsI GCNGC 4 cut(s) 221, 332, 525, 563
Bme1390I CCNGG 1 cut(s) 586
Bme18I GGWCC 1 cut(s) 361
BmgT120I GGNCC 3 cut(s) 350, 351, 361
BmiI GGNNCC 3 cut(s) 352, 362, 444
BmrFI CCNGG 1 cut(s) 586
BpiI GAAGAC 1 cut(s) 613
BpmI CTGGAG 1 cut(s) 568
BsaBI GATNNNNATC 1 cut(s) 57
BsaJI CCNNGG 1 cut(s) 331
Bsc4I CCNNNNNNNGG 1 cut(s) 233
Bse1I ACTGG 2 cut(s) 275, 471
Bse8I GATNNNNATC 1 cut(s) 57
BseBI CCWGG 1 cut(s) 586
BseDI CCNNGG 1 cut(s) 331
BseJI GATNNNNATC 1 cut(s) 57
BseLI CCNNNNNNNGG 1 cut(s) 233
BseMII CTCAG 4 cut(s) 448, 462, 507, 548
BseNI ACTGG 2 cut(s) 275, 471
BseRI GAGGAG 3 cut(s) 512, 515, 518
BseSI GKGCMC 1 cut(s) 354
BseXI GCAGC 2 cut(s) 535, 573
BsgI GTGCAG 1 cut(s) 542
Bsh1236I CGCG 1 cut(s) 333
Bsh1285I CGRYCG 1 cut(s) 70
BshFI GGCC 1 cut(s) 352
BsiEI CGRYCG 1 cut(s) 70
BsiWI CGTACG 1 cut(s) 345
BslFI GGGAC 2 cut(s) 21, 374
BslI CCNNNNNNNGG 1 cut(s) 233
BsmAI GTCTC 1 cut(s) 457
BsmFI GGGAC 2 cut(s) 21, 374
BsnI GGCC 1 cut(s) 352
Bsp120I GGGCCC 1 cut(s) 350
Bsp1286I GDGCHC 1 cut(s) 354
Bsp143I GATC 8 cut(s) 25, 52, 58, 67, 430, 528, 703, 708
BspACI CCGC 4 cut(s) 107, 220, 331, 333
BspANI GGCC 1 cut(s) 352
BspCNI CTCAG 4 cut(s) 447, 463, 506, 549
BspFNI CGCG 1 cut(s) 333
BspHI TCATGA 1 cut(s) 55
BspLI GGNNCC 3 cut(s) 352, 362, 444
BspMI ACCTGC 1 cut(s) 150
BspPI GGATC 2 cut(s) 438, 711
BsrI ACTGG 2 cut(s) 275, 471
BssECI CCNNGG 1 cut(s) 331
BssMI GATC 8 cut(s) 25, 52, 58, 67, 430, 528, 703, 708
Bst2UI CCWGG 1 cut(s) 586
Bst4CI ACNGT 4 cut(s) 32, 77, 597, 632
Bst6I CTCTTC 1 cut(s) 455
BstC8I GCNNGC 1 cut(s) 388
BstDEI CTNAG 4 cut(s) 434, 471, 493, 557
BstDSI CCRYGG 1 cut(s) 331
BstFNI CGCG 1 cut(s) 333
BstKTI GATC 8 cut(s) 28, 55, 61, 70, 433, 531, 706, 711
BstMAI GTCTC 1 cut(s) 457
BstMBI GATC 8 cut(s) 25, 52, 58, 67, 430, 528, 703, 708
BstMCI CGRYCG 1 cut(s) 70
BstNI CCWGG 1 cut(s) 586
BstNSI RCATGY 1 cut(s) 13
BstSCI CCNGG 1 cut(s) 584
BstSLI GKGCMC 1 cut(s) 354
BstUI CGCG 1 cut(s) 333
BstV1I GCAGC 2 cut(s) 535, 573
BstV2I GAAGAC 1 cut(s) 613
BstX2I RGATCY 1 cut(s) 430
BstYI RGATCY 1 cut(s) 430
BsuRI GGCC 1 cut(s) 352
BtgI CCRYGG 1 cut(s) 331
BtsIMutI CAGTG 3 cut(s) 637, 678, 733
BveI ACCTGC 1 cut(s) 150
Cac8I GCNNGC 1 cut(s) 388
CciI TCATGA 1 cut(s) 55
Cfr13I GGNCC 3 cut(s) 350, 351, 361
Cfr42I CCGCGG 1 cut(s) 334
CseI GACGC 1 cut(s) 598
Csp6I GTAC 2 cut(s) 346, 598
CviAII CATG 4 cut(s) 10, 23, 56, 365
CviJI RGCY 8 cut(s) 322, 352, 390, 445, 470, 538, 564, 611
CviKI_1 RGCY 8 cut(s) 322, 352, 390, 445, 470, 538, 564, 611
CviQI GTAC 2 cut(s) 346, 598
DdeI CTNAG 4 cut(s) 434, 471, 493, 557
DpnI GATC 8 cut(s) 27, 54, 60, 69, 432, 530, 705, 710
DpnII GATC 8 cut(s) 25, 52, 58, 67, 430, 528, 703, 708
Eam1104I CTCTTC 1 cut(s) 455
EarI CTCTTC 1 cut(s) 455
EciI GGCGGA 1 cut(s) 96
Eco24I GRGCYC 1 cut(s) 354
Eco47I GGWCC 1 cut(s) 361
Eco57I CTGAAG 1 cut(s) 625
EcoO109I RGGNCCY 1 cut(s) 351
EcoRII CCWGG 1 cut(s) 584
EcoT38I GRGCYC 1 cut(s) 354
FaeI CATG 4 cut(s) 13, 26, 59, 368
FalI AAGNNNNNCTT 2 cut(s) 659, 691
FaqI GGGAC 2 cut(s) 21, 374
FatI CATG 4 cut(s) 9, 22, 55, 364
FbaI TGATCA 3 cut(s) 25, 52, 58
FblI GTMKAC 1 cut(s) 339
Fnu4HI GCNGC 4 cut(s) 220, 331, 524, 562
FriOI GRGCYC 1 cut(s) 354
Fsp4HI GCNGC 4 cut(s) 220, 331, 524, 562
FspBI CTAG 2 cut(s) 180, 371
GluI GCNGC 4 cut(s) 220, 331, 524, 562
GsuI CTGGAG 1 cut(s) 568
HaeIII GGCC 1 cut(s) 352
HgaI GACGC 1 cut(s) 598
Hin1II CATG 4 cut(s) 13, 26, 59, 368
HincII GTYRAC 1 cut(s) 485
HindII GTYRAC 1 cut(s) 485
HindIII AAGCTT 2 cut(s) 388, 536
HinfI GANTC 3 cut(s) 237, 336, 553
HphI GGTGA 1 cut(s) 92
Hpy166II GTNNAC 4 cut(s) 211, 280, 340, 485
Hpy188I TCNGA 3 cut(s) 496, 558, 708
Hpy188III TCNNGA 8 cut(s) 56, 62, 71, 259, 436, 625, 643, 735
Hpy8I GTNNAC 4 cut(s) 211, 280, 340, 485
Hpy99I CGWCG 1 cut(s) 68
HpyAV CCTTC 1 cut(s) 433
HpyCH4III ACNGT 4 cut(s) 32, 77, 597, 632
HpyCH4IV ACGT 2 cut(s) 213, 697
HpyCH4V TGCA 2 cut(s) 293, 523
HpyF3I CTNAG 4 cut(s) 434, 471, 493, 557
HpySE526I ACGT 2 cut(s) 213, 697
Hsp92II CATG 4 cut(s) 13, 26, 59, 368
Ksp22I TGATCA 3 cut(s) 25, 52, 58
KspI CCGCGG 1 cut(s) 334
Kzo9I GATC 8 cut(s) 25, 52, 58, 67, 430, 528, 703, 708
LmnI GCTCC 1 cut(s) 442
Lsp1109I GCAGC 2 cut(s) 535, 573
MaeI CTAG 2 cut(s) 180, 371
MaeII ACGT 2 cut(s) 213, 697
MaeIII GTNAC 5 cut(s) 40, 71, 156, 169, 632
MalI GATC 8 cut(s) 27, 54, 60, 69, 432, 530, 705, 710
MboI GATC 8 cut(s) 25, 52, 58, 67, 430, 528, 703, 708
MboII GAAGA 6 cut(s) 320, 472, 613, 631, 667, 679
MflI RGATCY 1 cut(s) 430
MhlI GDGCHC 1 cut(s) 354
MluCI AATT 2 cut(s) 97, 203
MlyI GAGTC 1 cut(s) 345
MnlI CCTC 8 cut(s) 55, 421, 490, 493, 496, 562, 592, 714
MroXI GAANNNNTTC 2 cut(s) 671, 674
MseI TTAA 4 cut(s) 93, 270, 419, 606
MspA1I CMGCKG 1 cut(s) 333
MspR9I CCNGG 1 cut(s) 586
MvaI CCWGG 1 cut(s) 586
MvnI CGCG 1 cut(s) 333
NdeII GATC 8 cut(s) 25, 52, 58, 67, 430, 528, 703, 708
NlaIII CATG 4 cut(s) 13, 26, 59, 368
NlaIV GGNNCC 3 cut(s) 352, 362, 444
NmuCI GTSAC 3 cut(s) 71, 169, 632
NspI RCATGY 1 cut(s) 13
PagI TCATGA 1 cut(s) 55
PaqCI CACCTGC 1 cut(s) 150
PdmI GAANNNNTTC 2 cut(s) 671, 674
PfeI GAWTC 2 cut(s) 237, 553
Pfl23II CGTACG 1 cut(s) 345
PfoI TCCNGGA 1 cut(s) 584
PkrI GCNGC 4 cut(s) 221, 332, 525, 563
Ple19I CGATCG 1 cut(s) 70
PleI GAGTC 1 cut(s) 344
PpsI GAGTC 1 cut(s) 344
PshBI ATTAAT 1 cut(s) 93
Psp6I CCWGG 1 cut(s) 584
PspGI CCWGG 1 cut(s) 584
PspLI CGTACG 1 cut(s) 345
PspN4I GGNNCC 3 cut(s) 352, 362, 444
PspOMI GGGCCC 1 cut(s) 350
PspPI GGNCC 3 cut(s) 350, 351, 361
PsuI RGATCY 1 cut(s) 430
PvuI CGATCG 1 cut(s) 70
RsaI GTAC 2 cut(s) 347, 599
RsaNI GTAC 2 cut(s) 346, 598
SacII CCGCGG 1 cut(s) 334
SaqAI TTAA 4 cut(s) 93, 270, 419, 606
SatI GCNGC 4 cut(s) 220, 331, 524, 562
Sau3AI GATC 8 cut(s) 25, 52, 58, 67, 430, 528, 703, 708
Sau96I GGNCC 3 cut(s) 350, 351, 361
SchI GAGTC 1 cut(s) 345
ScrFI CCNGG 1 cut(s) 586
SduI GDGCHC 1 cut(s) 354
Sfr303I CCGCGG 1 cut(s) 334
SgrBI CCGCGG 1 cut(s) 334
SinI GGWCC 1 cut(s) 361
Sse9I AATT 2 cut(s) 97, 203
SsiI CCGC 4 cut(s) 107, 220, 331, 333
SspMI CTAG 2 cut(s) 180, 371
StyD4I CCNGG 1 cut(s) 584
TaaI ACNGT 4 cut(s) 32, 77, 597, 632
TaiI ACGT 2 cut(s) 216, 700
TaqI TCGA 1 cut(s) 624
TasI AATT 2 cut(s) 97, 203
TauI GCSGC 2 cut(s) 222, 333
TfiI GAWTC 2 cut(s) 237, 553
Tru1I TTAA 4 cut(s) 93, 270, 419, 606
Tru9I TTAA 4 cut(s) 93, 270, 419, 606
TscAI CASTG 3 cut(s) 637, 685, 733
TseFI GTSAC 3 cut(s) 71, 169, 632
TseI GCWGC 2 cut(s) 523, 561
Tsp45I GTSAC 3 cut(s) 71, 169, 632
TspDTI ATGAA 1 cut(s) 229
TspRI CASTG 3 cut(s) 637, 685, 733
VpaK11BI GGWCC 1 cut(s) 361
VspI ATTAAT 1 cut(s) 93
XapI RAATTY 1 cut(s) 203
XceI RCATGY 1 cut(s) 13
XmiI GTMKAC 1 cut(s) 339
XmnI GAANNNNTTC 2 cut(s) 671, 674
XspI CTAG 2 cut(s) 180, 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.