MD06G1151400.v1.1

Belongs to the sulfotransferase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0019270
Physical Location & Seq
Forward (+)
29519766 .. 29520712
947 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1151400.v1.1.491

Sequence Viewer

Length: 876 bp
ATGCTGCTTCAAGATCATTTCAAAGCTGGTGCAAGTAGTACGTCCAATATATTCTTGGCTTCGTTTCCGAAATCAGGTACAACATGGCATAGAGCACTCATTTTTGCTACCATCAATAGAACCCTGCATGATGTTGCCTCTCCTCATCACCCTTTGCTCACCACGGGGCCCCACGACTGTTTTCCGTACTTACAGTTGGATGCATATCTATATAATAATAATATGCATGAACATCGTGATCATGATCATCACAGCCGTTCATTGATGTCGCATGATGATCGTGATCATGATGGTGATGGTAGTACTAACATTGCTACAACTACTAGACTCTTTGCAACCCACAAGCCTTACTCTTTGTTACCAAAATCAGTGACTACTAATAATATTGGTGGGTCAAAATTTGTGTACATTTGCCGCAACCCAAAAGATGTTTGTATTTCTTCATGGAACTTCATGAACAAATTGAAGTCCCAAGTATGTACACCCCTCTCACTAGAAGAAGCATTTGAGCTTTTTTGCCAAGGATTCTCTGACTATGGACCCTTTTGGGACCATGTGCTAGGTTTCTTGAAAGCAAGCCTGGAATTTCCAGAGAAAATATTGTTCTTAAAATATGAGGATCTGAAAAAAGAGCCGGATGTGCAAGTGAAGAGATTGGCTGAGTTCTTGGGCCAACCTTTTTCCTTGGAGGAAGAGAGCCAAGGGGTGGTGCAGCAGATCATCAAGCTCTGTGGTTTCGAGAATTTGAGCGGTCTGGAGGAAAGGTCAAGTGGGGACTGGAAGAACTTTTTCACTGAAGACATGGCAAAACGTATGGATCAAATCACAGATGACAAGTTGAACGGTTCCGTATTGACATTTTATACAGATGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sulfotransfer_1

Protein Analysis

292

Amino Acids

33.19

Weight (kDa)

5.8

Isoelectric Point (pI)

41.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sulfotransfer_1 PF00685 16 - 283 1.2e-52 Sulfotransferase domain
Sulfotransfer_3 PF13469 17 - 227 2.8e-10 Sulfotransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 706
AccBSI CCGCTC 1 cut(s) 750
AciI CCGC 2 cut(s) 415, 750
AclWI GGATC 2 cut(s) 627, 825
AcsI RAATTY 3 cut(s) 398, 584, 742
AcuI CTGAAG 1 cut(s) 816
AfaI GTAC 6 cut(s) 40, 79, 188, 304, 407, 481
AfiI CCNNNNNNNGG 2 cut(s) 74, 706
AgsI TTSAA 5 cut(s) 11, 22, 466, 571, 841
AjnI CCWGG 1 cut(s) 579
AluBI AGCT 3 cut(s) 26, 511, 727
AluI AGCT 3 cut(s) 26, 511, 727
Alw21I GWGCWC 1 cut(s) 97
AlwI GGATC 2 cut(s) 627, 825
AoxI GGCC 2 cut(s) 167, 670
ApaI GGGCCC 1 cut(s) 171
ApeKI GCWGC 2 cut(s) 4, 712
ApoI RAATTY 3 cut(s) 398, 584, 742
Asp700I GAANNNNTTC 1 cut(s) 788
AspS9I GGNCC 5 cut(s) 167, 168, 539, 550, 670
AsuHPI GGTGA 3 cut(s) 140, 151, 305
AvaII GGWCC 2 cut(s) 539, 550
BaeGI GKGCMC 1 cut(s) 171
BanII GRGCYC 1 cut(s) 171
BbsI GAAGAC 1 cut(s) 804
Bbv12I GWGCWC 1 cut(s) 97
BbvI GCAGC 1 cut(s) 724
BccI CCATC 3 cut(s) 119, 284, 290
BceAI ACGGC 1 cut(s) 240
BcgI CGANNNNNNTGC 4 cut(s) 215, 249, 260, 294
BciT130I CCWGG 1 cut(s) 581
BclI TGATCA 3 cut(s) 238, 244, 283
BfaI CTAG 4 cut(s) 324, 494, 560, 874
BisI GCNGC 3 cut(s) 5, 415, 713
BlsI GCNGC 3 cut(s) 6, 416, 714
BmcAI AGTACT 1 cut(s) 304
Bme1390I CCNGG 1 cut(s) 581
Bme18I GGWCC 2 cut(s) 539, 550
BmgT120I GGNCC 5 cut(s) 167, 168, 539, 550, 670
BmiI GGNNCC 6 cut(s) 168, 169, 170, 541, 551, 847
BmrFI CCNGG 1 cut(s) 581
BmsI GCATC 2 cut(s) 190, 859
BpiI GAAGAC 1 cut(s) 804
BpmI CTGGAG 1 cut(s) 776
BsaBI GATNNNNATC 3 cut(s) 204, 243, 282
BsaJI CCNNGG 4 cut(s) 162, 520, 684, 700
BsaXI ACNNNNNCTCC 2 cut(s) 749, 779
Bsc4I CCNNNNNNNGG 2 cut(s) 74, 706
Bse1I ACTGG 1 cut(s) 782
Bse3DI GCAATG 1 cut(s) 309
Bse8I GATNNNNATC 3 cut(s) 204, 243, 282
BseBI CCWGG 1 cut(s) 581
BseDI CCNNGG 4 cut(s) 162, 520, 684, 700
BseGI GGATG 2 cut(s) 205, 643
BseJI GATNNNNATC 3 cut(s) 204, 243, 282
BseLI CCNNNNNNNGG 2 cut(s) 74, 706
BseMI GCAATG 1 cut(s) 309
BseMII CTCAG 1 cut(s) 651
BseNI ACTGG 1 cut(s) 782
BseRI GAGGAG 1 cut(s) 132
BseSI GKGCMC 1 cut(s) 171
BseXI GCAGC 1 cut(s) 724
BsgI GTGCAG 1 cut(s) 731
BshFI GGCC 2 cut(s) 169, 672
BsiHKAI GWGCWC 1 cut(s) 97
BsiSI CCGG 1 cut(s) 635
BslFI GGGAC 3 cut(s) 454, 563, 788
BslI CCNNNNNNNGG 2 cut(s) 74, 706
BsmFI GGGAC 3 cut(s) 454, 563, 788
BsnI GGCC 2 cut(s) 169, 672
Bsp120I GGGCCC 1 cut(s) 167
Bsp1286I GDGCHC 2 cut(s) 97, 171
Bsp1407I TGTACA 2 cut(s) 405, 479
Bsp143I GATC 8 cut(s) 13, 238, 244, 277, 283, 619, 717, 817
BspACI CCGC 2 cut(s) 415, 750
BspANI GGCC 2 cut(s) 169, 672
BspCNI CTCAG 1 cut(s) 652
BspHI TCATGA 3 cut(s) 241, 286, 453
BspLI GGNNCC 6 cut(s) 168, 169, 170, 541, 551, 847
BspPI GGATC 2 cut(s) 627, 825
BsrBI CCGCTC 1 cut(s) 750
BsrDI GCAATG 1 cut(s) 309
BsrGI TGTACA 2 cut(s) 405, 479
BsrI ACTGG 1 cut(s) 782
BssECI CCNNGG 4 cut(s) 162, 520, 684, 700
BssMI GATC 8 cut(s) 13, 238, 244, 277, 283, 619, 717, 817
BssT1I CCWWGG 3 cut(s) 520, 684, 700
Bst2UI CCWGG 1 cut(s) 581
Bst4CI ACNGT 3 cut(s) 179, 195, 845
Bst6I CTCTTC 2 cut(s) 644, 687
BstAUI TGTACA 2 cut(s) 405, 479
BstC8I GCNNGC 1 cut(s) 577
BstDEI CTNAG 1 cut(s) 660
BstDSI CCRYGG 1 cut(s) 162
BstF5I GGATG 2 cut(s) 205, 643
BstKTI GATC 8 cut(s) 16, 241, 247, 280, 286, 622, 720, 820
BstMBI GATC 8 cut(s) 13, 238, 244, 277, 283, 619, 717, 817
BstMWI GCNNNNNNNGC 1 cut(s) 640
BstNI CCWGG 1 cut(s) 581
BstSCI CCNGG 1 cut(s) 579
BstSLI GKGCMC 1 cut(s) 171
BstV1I GCAGC 1 cut(s) 724
BstV2I GAAGAC 1 cut(s) 804
BstX2I RGATCY 1 cut(s) 619
BstYI RGATCY 1 cut(s) 619
BsuRI GGCC 2 cut(s) 169, 672
BtgI CCRYGG 1 cut(s) 162
BtsCI GGATG 2 cut(s) 205, 643
BtsIMutI CAGTG 2 cut(s) 375, 792
Cac8I GCNNGC 1 cut(s) 577
CciI TCATGA 3 cut(s) 241, 286, 453
Cfr13I GGNCC 5 cut(s) 167, 168, 539, 550, 670
Csp6I GTAC 6 cut(s) 39, 78, 187, 303, 406, 480
CspCI CAANNNNNGTGG 2 cut(s) 712, 747
CviQI GTAC 6 cut(s) 39, 78, 187, 303, 406, 480
DdeI CTNAG 1 cut(s) 660
DpnI GATC 8 cut(s) 15, 240, 246, 279, 285, 621, 719, 819
DpnII GATC 8 cut(s) 13, 238, 244, 277, 283, 619, 717, 817
Eam1104I CTCTTC 2 cut(s) 644, 687
EarI CTCTTC 2 cut(s) 644, 687
Eco130I CCWWGG 3 cut(s) 520, 684, 700
Eco24I GRGCYC 1 cut(s) 171
Eco47I GGWCC 2 cut(s) 539, 550
Eco57I CTGAAG 1 cut(s) 816
EcoO109I RGGNCCY 2 cut(s) 167, 168
EcoRII CCWGG 1 cut(s) 579
EcoT14I CCWWGG 3 cut(s) 520, 684, 700
EcoT22I ATGCAT 2 cut(s) 205, 228
EcoT38I GRGCYC 1 cut(s) 171
ErhI CCWWGG 3 cut(s) 520, 684, 700
FaqI GGGAC 3 cut(s) 454, 563, 788
FbaI TGATCA 3 cut(s) 238, 244, 283
Fnu4HI GCNGC 3 cut(s) 5, 415, 713
FokI GGATG 2 cut(s) 212, 650
FriOI GRGCYC 1 cut(s) 171
Fsp4HI GCNGC 3 cut(s) 5, 415, 713
FspBI CTAG 4 cut(s) 324, 494, 560, 874
GluI GCNGC 3 cut(s) 5, 415, 713
GsuI CTGGAG 1 cut(s) 776
HaeIII GGCC 2 cut(s) 169, 672
HapII CCGG 1 cut(s) 635
HinfI GANTC 2 cut(s) 327, 525
HpaII CCGG 1 cut(s) 635
HphI GGTGA 3 cut(s) 140, 151, 305
Hpy166II GTNNAC 2 cut(s) 406, 482
Hpy188I TCNGA 3 cut(s) 69, 532, 624
Hpy8I GTNNAC 2 cut(s) 406, 482
HpyCH4III ACNGT 3 cut(s) 179, 195, 845
HpyCH4IV ACGT 2 cut(s) 41, 811
HpyCH4V TGCA 7 cut(s) 32, 127, 203, 226, 335, 643, 712
HpyF10VI GCNNNNNNNGC 1 cut(s) 640
HpyF3I CTNAG 1 cut(s) 660
HpySE526I ACGT 2 cut(s) 41, 811
Ksp22I TGATCA 3 cut(s) 238, 244, 283
Kzo9I GATC 8 cut(s) 13, 238, 244, 277, 283, 619, 717, 817
LpnPI CCDG 9 cut(s) 12, 60, 137, 566, 593, 603, 648, 740, 763
Lsp1109I GCAGC 1 cut(s) 724
LweI GCATC 2 cut(s) 190, 859
MaeI CTAG 4 cut(s) 324, 494, 560, 874
MaeII ACGT 2 cut(s) 41, 811
MaeIII GTNAC 2 cut(s) 357, 370
MalI GATC 8 cut(s) 15, 240, 246, 279, 285, 621, 719, 819
MbiI CCGCTC 1 cut(s) 750
MboI GATC 8 cut(s) 13, 238, 244, 277, 283, 619, 717, 817
MboII GAAGA 6 cut(s) 432, 509, 661, 704, 793, 809
MflI RGATCY 1 cut(s) 619
MhlI GDGCHC 2 cut(s) 97, 171
MluCI AATT 4 cut(s) 398, 461, 584, 742
MlyI GAGTC 1 cut(s) 321
MmeI TCCRAC 1 cut(s) 177
MnlI CCTC 6 cut(s) 148, 153, 497, 610, 682, 751
Mph1103I ATGCAT 2 cut(s) 205, 228
MroXI GAANNNNTTC 1 cut(s) 788
MseI TTAA 1 cut(s) 608
MslI CAYNNNNRTG 1 cut(s) 291
MspI CCGG 1 cut(s) 635
MspR9I CCNGG 1 cut(s) 581
MvaI CCWGG 1 cut(s) 581
MwoI GCNNNNNNNGC 1 cut(s) 640
NdeII GATC 8 cut(s) 13, 238, 244, 277, 283, 619, 717, 817
NlaIV GGNNCC 6 cut(s) 168, 169, 170, 541, 551, 847
NmuCI GTSAC 1 cut(s) 370
NsiI ATGCAT 2 cut(s) 205, 228
PagI TCATGA 3 cut(s) 241, 286, 453
PdmI GAANNNNTTC 1 cut(s) 788
PfeI GAWTC 1 cut(s) 525
PflMI CCANNNNNTGG 1 cut(s) 706
PkrI GCNGC 3 cut(s) 6, 416, 714
PleI GAGTC 1 cut(s) 321
PpsI GAGTC 1 cut(s) 321
Psp6I CCWGG 1 cut(s) 579
PspGI CCWGG 1 cut(s) 579
PspN4I GGNNCC 6 cut(s) 168, 169, 170, 541, 551, 847
PspOMI GGGCCC 1 cut(s) 167
PspPI GGNCC 5 cut(s) 167, 168, 539, 550, 670
PsuI RGATCY 1 cut(s) 619
RsaI GTAC 6 cut(s) 40, 79, 188, 304, 407, 481
RsaNI GTAC 6 cut(s) 39, 78, 187, 303, 406, 480
RseI CAYNNNNRTG 1 cut(s) 291
SaqAI TTAA 1 cut(s) 608
SatI GCNGC 3 cut(s) 5, 415, 713
Sau3AI GATC 8 cut(s) 13, 238, 244, 277, 283, 619, 717, 817
Sau96I GGNCC 5 cut(s) 167, 168, 539, 550, 670
ScaI AGTACT 1 cut(s) 304
SchI GAGTC 1 cut(s) 321
ScrFI CCNGG 1 cut(s) 581
SduI GDGCHC 2 cut(s) 97, 171
SetI ASST 9 cut(s) 28, 44, 79, 513, 565, 679, 729, 767, 814
SfaNI GCATC 2 cut(s) 190, 859
SinI GGWCC 2 cut(s) 539, 550
SmiMI CAYNNNNRTG 1 cut(s) 291
Sse9I AATT 4 cut(s) 398, 461, 584, 742
SsiI CCGC 2 cut(s) 415, 750
SspI AATATT 2 cut(s) 385, 600
SspMI CTAG 4 cut(s) 324, 494, 560, 874
StyD4I CCNGG 1 cut(s) 579
StyI CCWWGG 3 cut(s) 520, 684, 700
TaaI ACNGT 3 cut(s) 179, 195, 845
TaiI ACGT 2 cut(s) 44, 814
TaqI TCGA 1 cut(s) 738
TasI AATT 4 cut(s) 398, 461, 584, 742
TatI WGTACW 3 cut(s) 302, 405, 479
TauI GCSGC 1 cut(s) 417
TfiI GAWTC 1 cut(s) 525
Tru1I TTAA 1 cut(s) 608
Tru9I TTAA 1 cut(s) 608
TscAI CASTG 2 cut(s) 375, 799
TseFI GTSAC 1 cut(s) 370
TseI GCWGC 2 cut(s) 4, 712
Tsp45I GTSAC 1 cut(s) 370
TspDTI ATGAA 5 cut(s) 243, 249, 432, 442, 470
TspGWI ACGGA 2 cut(s) 174, 838
TspRI CASTG 2 cut(s) 375, 799
Van91I CCANNNNNTGG 1 cut(s) 706
VpaK11BI GGWCC 2 cut(s) 539, 550
XapI RAATTY 3 cut(s) 398, 584, 742
XcmI CCANNNNNNNNNTGG 1 cut(s) 52
XmnI GAANNNNTTC 1 cut(s) 788
XspI CTAG 4 cut(s) 324, 494, 560, 874
ZrmI AGTACT 1 cut(s) 304
Zsp2I ATGCAT 2 cut(s) 205, 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.