MD06G1166000.v1.1

zinc ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0016329
Physical Location & Seq
Reverse (-)
30595678 .. 30597028
1351 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1166000.v1.1.491

Sequence Viewer

Length: 588 bp
ATGGCCTCCACAGCGGTCATCGATGTCCGCTATTTCGGAACCTCTAAGGTTAGAGCTGCCGGCAGAGTGAGGCATCTCAGTGCTCGTGTTGATAACAATGGCGAACTGGATCTCGACATGGGAGGATTGCGGAGAGAAATTCGCAGCCTTTGTGCCCTCCCACCCGCTGCTGACATTACTCTGACGTACATTGATGAAAACTACGATGTAGTTACTCTTGTGGACGATGATGATCTGCGGGAAGCAATGAGGCAGCGTCTGGAACCCTTCAGGATCGATGTGCTGATGAACGATGACAAGGAAGACCGCGGTAAAAACTCAAGCTCCGAAAAATGTCTCGTGACGGATGCTGTAAATGCGACAAGTGATGTGAGTAATAGCAATGTGTTTGATGAATTCCATCTGGCAAATTATGATCACCCTGTTAGTCCTAGGGTTAAACAATCTGAAGACGAATGTGGTATGATGTGCGACGCAGGCATTCGCTGTGGCGCCTGCAACGACAAGGTTGCGAAATTGTGGTTAGCGGCGTTGGCCCTCGTAGTGTCAGTTTGGTTATTATCCCGGCGACATGGATCGGAGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HECT MIB_HERC2 Neuralized ZZ zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

196

Amino Acids

21.65

Weight (kDa)

4.93

Isoelectric Point (pI)

48.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 40 - 85 3.5e-08 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016329)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g06081
malus_domestica MD06G1166000.v1.1
prunus_persica Prupe.5G165900_v2.0.a1
pyrus_communis pycom06g14850
rosa_chinensis RchiOBHm_Chr7g0192771
rosa_laevigata RLG00000004330
rosa_multiflora Rmu_sc0002040.1_g000018
rosa_roxburghii Rroxscaffold_3G00262700
rosa_rugosa Rorug07G0004500 Rorug07G0004500
rosa_samantha Rh7AG129600 Rh7BG130900 Rh7CG134300 Rh7DG131800
rosa_wichuraiana Rw7G011090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 491
AccII CGCG 1 cut(s) 309
AciI CCGC 8 cut(s) 14, 28, 130, 165, 238, 307, 309, 527
AclWI GGATC 3 cut(s) 117, 281, 583
AcsI RAATTY 2 cut(s) 138, 395
AcuI CTGAAG 2 cut(s) 253, 468
AcyI GRCGYC 1 cut(s) 492
AfaI GTAC 1 cut(s) 188
AluBI AGCT 2 cut(s) 56, 324
AluI AGCT 2 cut(s) 56, 324
Alw21I GWGCWC 1 cut(s) 85
Alw26I GTCTC 1 cut(s) 341
AlwI GGATC 3 cut(s) 117, 281, 583
AlwNI CAGNNNCTG 1 cut(s) 259
AoxI GGCC 2 cut(s) 3, 534
ApeKI GCWGC 4 cut(s) 56, 144, 167, 253
ApoI RAATTY 2 cut(s) 138, 395
AspA2I CCTAGG 1 cut(s) 431
AspLEI GCGC 1 cut(s) 494
AspS9I GGNCC 1 cut(s) 535
AsuC2I CCSGG 1 cut(s) 565
AsuHPI GGTGA 1 cut(s) 410
AvrII CCTAGG 1 cut(s) 431
BaeGI GKGCMC 1 cut(s) 157
BanI GGYRCC 1 cut(s) 491
BauI CACGAG 2 cut(s) 84, 338
BbsI GAAGAC 2 cut(s) 309, 456
Bbv12I GWGCWC 1 cut(s) 85
BbvI GCAGC 4 cut(s) 43, 154, 156, 265
BccI CCATC 1 cut(s) 408
BcgI CGANNNNNNTGC 2 cut(s) 491, 525
BclI TGATCA 1 cut(s) 415
BcnI CCSGG 1 cut(s) 565
BcoDI GTCTC 1 cut(s) 341
BfaI CTAG 1 cut(s) 432
BfoI RGCGCY 1 cut(s) 495
BisI GCNGC 5 cut(s) 57, 145, 168, 254, 528
BlnI CCTAGG 1 cut(s) 431
BlsI GCNGC 5 cut(s) 58, 146, 169, 255, 529
Bme1390I CCNGG 1 cut(s) 565
BmgT120I GGNCC 1 cut(s) 535
BmiI GGNNCC 3 cut(s) 40, 264, 493
BmrFI CCNGG 1 cut(s) 565
BmsI GCATC 2 cut(s) 82, 337
BpiI GAAGAC 2 cut(s) 309, 456
BpuEI CTTGAG 1 cut(s) 304
BpuMI CCSGG 1 cut(s) 565
Bsa29I ATCGAT 2 cut(s) 21, 276
BsaBI GATNNNNATC 1 cut(s) 231
BsaHI GRCGYC 1 cut(s) 492
BsaJI CCNNGG 2 cut(s) 307, 431
BsaXI ACNNNNNCTCC 2 cut(s) 308, 338
Bse118I RCCGGY 1 cut(s) 59
Bse1I ACTGG 1 cut(s) 111
Bse3DI GCAATG 2 cut(s) 252, 388
Bse8I GATNNNNATC 1 cut(s) 231
BseCI ATCGAT 2 cut(s) 21, 276
BseDI CCNNGG 2 cut(s) 307, 431
BseGI GGATG 1 cut(s) 352
BseJI GATNNNNATC 1 cut(s) 231
BseMI GCAATG 2 cut(s) 252, 388
BseMII CTCAG 1 cut(s) 91
BseNI ACTGG 1 cut(s) 111
BseSI GKGCMC 1 cut(s) 157
BseXI GCAGC 4 cut(s) 43, 154, 156, 265
Bsh1236I CGCG 1 cut(s) 309
BshFI GGCC 2 cut(s) 5, 536
BshNI GGYRCC 1 cut(s) 491
BshVI ATCGAT 2 cut(s) 21, 276
BsiHKAI GWGCWC 1 cut(s) 85
BsiSI CCGG 2 cut(s) 60, 565
BsmAI GTCTC 1 cut(s) 341
BsmI GAATGC 1 cut(s) 480
BsnI GGCC 2 cut(s) 5, 536
Bsp1286I GDGCHC 2 cut(s) 85, 157
Bsp143I GATC 5 cut(s) 109, 232, 273, 415, 575
BspACI CCGC 8 cut(s) 14, 28, 130, 165, 238, 307, 309, 527
BspANI GGCC 2 cut(s) 5, 536
BspCNI CTCAG 1 cut(s) 90
BspDI ATCGAT 2 cut(s) 21, 276
BspFNI CGCG 1 cut(s) 309
BspLI GGNNCC 3 cut(s) 40, 264, 493
BspPI GGATC 3 cut(s) 117, 281, 583
BspT107I GGYRCC 1 cut(s) 491
BsrDI GCAATG 2 cut(s) 252, 388
BsrFI RCCGGY 1 cut(s) 59
BsrI ACTGG 1 cut(s) 111
BssAI RCCGGY 1 cut(s) 59
BssECI CCNNGG 2 cut(s) 307, 431
BssMI GATC 5 cut(s) 109, 232, 273, 415, 575
BssNI GRCGYC 1 cut(s) 492
BssSI CACGAG 2 cut(s) 84, 338
BssT1I CCWWGG 1 cut(s) 431
Bst2BI CACGAG 2 cut(s) 84, 338
BstACI GRCGYC 1 cut(s) 492
BstC8I GCNNGC 3 cut(s) 61, 478, 496
BstDEI CTNAG 2 cut(s) 45, 77
BstDSI CCRYGG 1 cut(s) 307
BstF5I GGATG 1 cut(s) 352
BstFNI CGCG 1 cut(s) 309
BstH2I RGCGCY 1 cut(s) 495
BstHHI GCGC 1 cut(s) 494
BstKTI GATC 5 cut(s) 112, 235, 276, 418, 578
BstMAI GTCTC 1 cut(s) 341
BstMBI GATC 5 cut(s) 109, 232, 273, 415, 575
BstMWI GCNNNNNNNGC 4 cut(s) 11, 356, 477, 533
BstSCI CCNGG 1 cut(s) 563
BstSLI GKGCMC 1 cut(s) 157
BstUI CGCG 1 cut(s) 309
BstV1I GCAGC 4 cut(s) 43, 154, 156, 265
BstV2I GAAGAC 2 cut(s) 309, 456
BstX2I RGATCY 1 cut(s) 109
BstYI RGATCY 1 cut(s) 109
Bsu15I ATCGAT 2 cut(s) 21, 276
BsuRI GGCC 2 cut(s) 5, 536
BsuTUI ATCGAT 2 cut(s) 21, 276
BtgI CCRYGG 1 cut(s) 307
BtsCI GGATG 1 cut(s) 352
BtsIMutI CAGTG 1 cut(s) 85
Cac8I GCNNGC 3 cut(s) 61, 478, 496
CaiI CAGNNNCTG 1 cut(s) 259
CfoI GCGC 1 cut(s) 494
Cfr10I RCCGGY 1 cut(s) 59
Cfr13I GGNCC 1 cut(s) 535
Cfr42I CCGCGG 1 cut(s) 310
ClaI ATCGAT 2 cut(s) 21, 276
CseI GACGC 2 cut(s) 245, 482
Csp6I GTAC 1 cut(s) 187
CviAII CATG 2 cut(s) 118, 572
CviJI RGCY 5 cut(s) 5, 56, 147, 324, 536
CviKI_1 RGCY 5 cut(s) 5, 56, 147, 324, 536
CviQI GTAC 1 cut(s) 187
DdeI CTNAG 2 cut(s) 45, 77
DinI GGCGCC 1 cut(s) 493
DpnI GATC 5 cut(s) 111, 234, 275, 417, 577
DpnII GATC 5 cut(s) 109, 232, 273, 415, 575
Eco130I CCWWGG 1 cut(s) 431
Eco57I CTGAAG 2 cut(s) 253, 468
EcoRI GAATTC 1 cut(s) 395
EcoT14I CCWWGG 1 cut(s) 431
EgeI GGCGCC 1 cut(s) 493
EheI GGCGCC 1 cut(s) 493
ErhI CCWWGG 1 cut(s) 431
FaeI CATG 2 cut(s) 121, 575
FaiI YATR 4 cut(s) 119, 414, 464, 573
FatI CATG 2 cut(s) 117, 571
FauI CCCGC 2 cut(s) 172, 231
FbaI TGATCA 1 cut(s) 415
Fnu4HI GCNGC 5 cut(s) 57, 145, 168, 254, 528
FokI GGATG 1 cut(s) 359
Fsp4HI GCNGC 5 cut(s) 57, 145, 168, 254, 528
FspBI CTAG 1 cut(s) 432
GlaI GCGC 1 cut(s) 493
GluI GCNGC 5 cut(s) 57, 145, 168, 254, 528
HaeII RGCGCY 1 cut(s) 495
HaeIII GGCC 2 cut(s) 5, 536
HapII CCGG 2 cut(s) 60, 565
HgaI GACGC 2 cut(s) 245, 482
HhaI GCGC 1 cut(s) 494
Hin1I GRCGYC 1 cut(s) 492
Hin1II CATG 2 cut(s) 121, 575
Hin6I GCGC 1 cut(s) 492
HinP1I GCGC 1 cut(s) 492
HpaII CCGG 2 cut(s) 60, 565
HphI GGTGA 1 cut(s) 410
Hpy166II GTNNAC 1 cut(s) 223
Hpy188I TCNGA 5 cut(s) 38, 183, 328, 448, 580
Hpy188III TCNNGA 4 cut(s) 113, 260, 271, 340
Hpy8I GTNNAC 1 cut(s) 223
Hpy99I CGWCG 1 cut(s) 476
HpyAV CCTTC 1 cut(s) 277
HpyCH4IV ACGT 1 cut(s) 185
HpyCH4V TGCA 1 cut(s) 498
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 356, 477, 533
HpyF3I CTNAG 2 cut(s) 45, 77
HpySE526I ACGT 1 cut(s) 185
Hsp92I GRCGYC 1 cut(s) 492
Hsp92II CATG 2 cut(s) 121, 575
HspAI GCGC 1 cut(s) 492
KasI GGCGCC 1 cut(s) 491
KroI GCCGGC 1 cut(s) 59
KroNI GCCGGC 1 cut(s) 61
Ksp22I TGATCA 1 cut(s) 415
KspI CCGCGG 1 cut(s) 310
Kzo9I GATC 5 cut(s) 109, 232, 273, 415, 575
LmnI GCTCC 1 cut(s) 329
LpnPI CCDG 9 cut(s) 73, 92, 245, 256, 389, 435, 462, 508, 578
Lsp1109I GCAGC 4 cut(s) 43, 154, 156, 265
LweI GCATC 2 cut(s) 82, 337
MaeI CTAG 1 cut(s) 432
MaeII ACGT 1 cut(s) 185
MaeIII GTNAC 2 cut(s) 211, 340
MalI GATC 5 cut(s) 111, 234, 275, 417, 577
MboI GATC 5 cut(s) 109, 232, 273, 415, 575
MboII GAAGA 2 cut(s) 314, 461
MflI RGATCY 1 cut(s) 109
MhlI GDGCHC 2 cut(s) 85, 157
MluCI AATT 4 cut(s) 138, 395, 409, 515
Mly113I GGCGCC 1 cut(s) 492
MnlI CCTC 7 cut(s) 16, 52, 63, 116, 167, 243, 548
MroNI GCCGGC 1 cut(s) 59
MseI TTAA 1 cut(s) 438
MslI CAYNNNNRTG 1 cut(s) 78
MspA1I CMGCKG 3 cut(s) 14, 167, 309
MspI CCGG 2 cut(s) 60, 565
MspR9I CCNGG 1 cut(s) 565
Mva1269I GAATGC 1 cut(s) 480
MvnI CGCG 1 cut(s) 309
MwoI GCNNNNNNNGC 4 cut(s) 11, 356, 477, 533
NaeI GCCGGC 1 cut(s) 61
NarI GGCGCC 1 cut(s) 492
NciI CCSGG 1 cut(s) 565
NdeII GATC 5 cut(s) 109, 232, 273, 415, 575
NgoMIV GCCGGC 1 cut(s) 59
NlaIII CATG 2 cut(s) 121, 575
NlaIV GGNNCC 3 cut(s) 40, 264, 493
NmuCI GTSAC 1 cut(s) 340
PctI GAATGC 1 cut(s) 480
PdiI GCCGGC 1 cut(s) 61
PkrI GCNGC 5 cut(s) 58, 146, 169, 255, 529
PluTI GGCGCC 1 cut(s) 495
PspN4I GGNNCC 3 cut(s) 40, 264, 493
PspPI GGNCC 1 cut(s) 535
PstNI CAGNNNCTG 1 cut(s) 259
PsuI RGATCY 1 cut(s) 109
RsaI GTAC 1 cut(s) 188
RsaNI GTAC 1 cut(s) 187
RseI CAYNNNNRTG 1 cut(s) 78
SacII CCGCGG 1 cut(s) 310
SaqAI TTAA 1 cut(s) 438
SatI GCNGC 5 cut(s) 57, 145, 168, 254, 528
Sau3AI GATC 5 cut(s) 109, 232, 273, 415, 575
Sau96I GGNCC 1 cut(s) 535
ScrFI CCNGG 1 cut(s) 565
SduI GDGCHC 2 cut(s) 85, 157
SetI ASST 6 cut(s) 44, 51, 58, 188, 326, 510
SfaNI GCATC 2 cut(s) 82, 337
SfoI GGCGCC 1 cut(s) 493
Sfr303I CCGCGG 1 cut(s) 310
SgrBI CCGCGG 1 cut(s) 310
SmiMI CAYNNNNRTG 1 cut(s) 78
SmlI CTYRAG 1 cut(s) 319
SmoI CTYRAG 1 cut(s) 319
Sse9I AATT 4 cut(s) 138, 395, 409, 515
SsiI CCGC 8 cut(s) 14, 28, 130, 165, 238, 307, 309, 527
SspDI GGCGCC 1 cut(s) 491
SspMI CTAG 1 cut(s) 432
StyD4I CCNGG 1 cut(s) 563
StyI CCWWGG 1 cut(s) 431
TaiI ACGT 1 cut(s) 188
TaqI TCGA 3 cut(s) 21, 114, 276
TasI AATT 4 cut(s) 138, 395, 409, 515
TauI GCSGC 1 cut(s) 530
Tru1I TTAA 1 cut(s) 438
Tru9I TTAA 1 cut(s) 438
TscAI CASTG 1 cut(s) 85
TseFI GTSAC 1 cut(s) 340
TseI GCWGC 4 cut(s) 56, 144, 167, 253
Tsp45I GTSAC 1 cut(s) 340
TspDTI ATGAA 3 cut(s) 210, 302, 408
TspGWI ACGGA 1 cut(s) 359
TspRI CASTG 1 cut(s) 85
XapI RAATTY 2 cut(s) 138, 395
XmaJI CCTAGG 1 cut(s) 431
XspI CTAG 1 cut(s) 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.