Prupe.5G165900_v2.0.a1

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0016329
Physical Location & Seq
Reverse (-)
14385260 .. 14387371
2112 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G165900.1

Sequence Viewer

Length: 762 bp
ATGGCGTCCACCACAGTTATCAAGGTGCGATATTTTGGAACTTCCAAGGTTAAACTTGGGGACAAACTGAGGTATTTCGAAGCTCGTGTTGATAAGAATGGTGAACTTGATCTTAACATGGCTGGATTGAGGGAAAAAATTTGCGGTCTTTTTCACCTCCCACCTGGTGCTGACATTACTCTAACATATATCGATGAAGACAGGGATGTAGTAACTCTCGTGGACGATGATGATCTGCGAGATGCGATGAGGCAGCATCTTAAGTCCTTTAGAATTGATGTACTGATGAATGATGACAAAGATGTTGGTAAATATTCAAGCTCCAAAAGAAGTAAAAGTCAAGGGAAGGATGCTGCTAATGTGACAAGTGATATGGAGATGGTGGTTCAACCTTACCCCGATTATTTTGATGATCTTGATCTCCTTGCTGATTCTGGGGAGACCAACAAGGCTACTAGCACAAGTTCTGCTTCTCCTCCAAATCATAATGAAGATATGAGCAGAAGCAATGCTCCTCCACATTATAATCACCCTTTTATTCCAACTGTGGCATGTCTTGAATCTGAAGCCGTAGTAGGTGGTATGATGTGCCACACTGGTTTTCGCTGCAGCATCTGTGGTTGCAATCCAATACTTGGATCCCGGTTCAAGTCGATAGTGATTGAAGATTATAGCCTCTGTCGAATCTGTGTTACGAGTCGGGGTAATGTGACTGATTACATTAGGATTGACCTCCCTGTGTCTCCAAAAGGGGATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 ZZ

Protein Analysis

254

Amino Acids

28.03

Weight (kDa)

5.26

Isoelectric Point (pI)

36.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016329)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g06081
malus_domestica MD06G1166000.v1.1
prunus_persica Prupe.5G165900_v2.0.a1
pyrus_communis pycom06g14850
rosa_chinensis RchiOBHm_Chr7g0192771
rosa_laevigata RLG00000004330
rosa_multiflora Rmu_sc0002040.1_g000018
rosa_roxburghii Rroxscaffold_3G00262700
rosa_rugosa Rorug07G0004500 Rorug07G0004500
rosa_samantha Rh7AG129600 Rh7BG130900 Rh7CG134300 Rh7DG131800
rosa_wichuraiana Rw7G011090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 525
AccB7I CCANNNNNTGG 1 cut(s) 635
AciI CCGC 1 cut(s) 144
AclWI GGATC 2 cut(s) 633, 646
AcsI RAATTY 1 cut(s) 138
AcuI CTGAAG 1 cut(s) 585
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 1 cut(s) 167
AfaI GTAC 1 cut(s) 282
AfiI CCNNNNNNNGG 1 cut(s) 635
AflII CTTAAG 1 cut(s) 260
AgsI TTSAA 5 cut(s) 318, 389, 560, 649, 665
AjnI CCWGG 1 cut(s) 163
AluBI AGCT 2 cut(s) 83, 321
AluI AGCT 2 cut(s) 83, 321
Alw26I GTCTC 2 cut(s) 434, 747
AlwI GGATC 2 cut(s) 633, 646
AlwNI CAGNNNCTG 1 cut(s) 615
ApeKI GCWGC 4 cut(s) 253, 353, 606, 609
ApoI RAATTY 1 cut(s) 138
AsuC2I CCSGG 1 cut(s) 643
AsuHPI GGTGA 3 cut(s) 113, 146, 521
AsuII TTCGAA 1 cut(s) 78
BamHI GGATCC 1 cut(s) 638
BauI CACGAG 2 cut(s) 84, 218
BbsI GAAGAC 1 cut(s) 204
BbvI GCAGC 4 cut(s) 265, 340, 593, 621
BccI CCATC 1 cut(s) 373
BceAI ACGGC 1 cut(s) 554
BciT130I CCWGG 1 cut(s) 165
BcnI CCSGG 1 cut(s) 643
BcoDI GTCTC 2 cut(s) 434, 747
BfaI CTAG 1 cut(s) 456
BfmI CTRYAG 1 cut(s) 607
BfrI CTTAAG 1 cut(s) 260
BisI GCNGC 4 cut(s) 254, 354, 607, 610
BlsI GCNGC 4 cut(s) 255, 355, 608, 611
Bme1390I CCNGG 2 cut(s) 165, 643
BmiI GGNNCC 1 cut(s) 640
BmrFI CCNGG 2 cut(s) 165, 643
BmsI GCATC 4 cut(s) 232, 265, 340, 621
BpiI GAAGAC 1 cut(s) 204
Bpu14I TTCGAA 1 cut(s) 78
BpuMI CCSGG 1 cut(s) 643
Bsa29I ATCGAT 1 cut(s) 192
BsaBI GATNNNNATC 2 cut(s) 231, 417
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 434
BsaJI CCNNGG 1 cut(s) 45
Bsc4I CCNNNNNNNGG 1 cut(s) 635
Bse1I ACTGG 1 cut(s) 601
Bse3DI GCAATG 1 cut(s) 514
Bse8I GATNNNNATC 2 cut(s) 231, 417
BseBI CCWGG 1 cut(s) 165
BseCI ATCGAT 1 cut(s) 192
BseDI CCNNGG 1 cut(s) 45
BseGI GGATG 3 cut(s) 211, 355, 760
BseJI GATNNNNATC 2 cut(s) 231, 417
BseLI CCNNNNNNNGG 1 cut(s) 635
BseMI GCAATG 1 cut(s) 514
BseMII CTCAG 1 cut(s) 59
BseNI ACTGG 1 cut(s) 601
BseRI GAGGAG 2 cut(s) 465, 504
BseXI GCAGC 4 cut(s) 265, 340, 593, 621
BshVI ATCGAT 1 cut(s) 192
BsiSI CCGG 1 cut(s) 643
BslFI GGGAC 1 cut(s) 74
BslI CCNNNNNNNGG 1 cut(s) 635
BsmAI GTCTC 2 cut(s) 434, 747
BsmFI GGGAC 1 cut(s) 74
Bso31I GGTCTC 1 cut(s) 434
Bsp119I TTCGAA 1 cut(s) 78
Bsp143I GATC 5 cut(s) 109, 232, 412, 418, 638
BspACI CCGC 1 cut(s) 144
BspCNI CTCAG 1 cut(s) 60
BspDI ATCGAT 1 cut(s) 192
BspLI GGNNCC 1 cut(s) 640
BspMAI CTGCAG 1 cut(s) 611
BspPI GGATC 2 cut(s) 633, 646
BspT104I TTCGAA 1 cut(s) 78
BspTI CTTAAG 1 cut(s) 260
BspTNI GGTCTC 1 cut(s) 434
BsrDI GCAATG 1 cut(s) 514
BsrI ACTGG 1 cut(s) 601
BssECI CCNNGG 1 cut(s) 45
BssMI GATC 5 cut(s) 109, 232, 412, 418, 638
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 2 cut(s) 84, 218
BssT1I CCWWGG 1 cut(s) 45
Bst2BI CACGAG 2 cut(s) 84, 218
Bst2UI CCWGG 1 cut(s) 165
Bst4CI ACNGT 2 cut(s) 16, 547
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 260
BstBI TTCGAA 1 cut(s) 78
BstDEI CTNAG 1 cut(s) 68
BstF5I GGATG 3 cut(s) 211, 355, 760
BstKTI GATC 5 cut(s) 112, 235, 415, 421, 641
BstMAI GTCTC 2 cut(s) 434, 747
BstMBI GATC 5 cut(s) 109, 232, 412, 418, 638
BstNI CCWGG 1 cut(s) 165
BstNSI RCATGY 1 cut(s) 555
BstSCI CCNGG 2 cut(s) 163, 641
BstSFI CTRYAG 1 cut(s) 607
BstV1I GCAGC 4 cut(s) 265, 340, 593, 621
BstV2I GAAGAC 1 cut(s) 204
BstX2I RGATCY 1 cut(s) 638
BstYI RGATCY 1 cut(s) 638
Bsu15I ATCGAT 1 cut(s) 192
BsuTUI ATCGAT 1 cut(s) 192
BtgZI GCGATG 1 cut(s) 260
BtsCI GGATG 3 cut(s) 211, 355, 760
BtsIMutI CAGTG 1 cut(s) 594
CaiI CAGNNNCTG 1 cut(s) 615
ClaI ATCGAT 1 cut(s) 192
CsiI ACCWGGT 1 cut(s) 163
Csp6I GTAC 1 cut(s) 281
CviAII CATG 2 cut(s) 118, 552
CviJI RGCY 6 cut(s) 83, 122, 321, 452, 569, 675
CviKI_1 RGCY 6 cut(s) 83, 122, 321, 452, 569, 675
CviQI GTAC 1 cut(s) 281
DdeI CTNAG 1 cut(s) 68
DpnI GATC 5 cut(s) 111, 234, 414, 420, 640
DpnII GATC 5 cut(s) 109, 232, 412, 418, 638
DraIII CACNNNGTG 1 cut(s) 167
Eco130I CCWWGG 1 cut(s) 45
Eco31I GGTCTC 1 cut(s) 434
Eco57I CTGAAG 1 cut(s) 585
EcoRII CCWGG 1 cut(s) 163
EcoT14I CCWWGG 1 cut(s) 45
ErhI CCWWGG 1 cut(s) 45
FaeI CATG 2 cut(s) 121, 555
FalI AAGNNNNNCTT 2 cut(s) 454, 486
FaqI GGGAC 1 cut(s) 74
FatI CATG 2 cut(s) 117, 551
Fnu4HI GCNGC 4 cut(s) 254, 354, 607, 610
FokI GGATG 2 cut(s) 218, 362
Fsp4HI GCNGC 4 cut(s) 254, 354, 607, 610
FspBI CTAG 1 cut(s) 456
GluI GCNGC 4 cut(s) 254, 354, 607, 610
HapII CCGG 1 cut(s) 643
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 121, 555
HinfI GANTC 4 cut(s) 431, 560, 684, 697
HpaII CCGG 1 cut(s) 643
HphI GGTGA 3 cut(s) 113, 146, 521
Hpy166II GTNNAC 3 cut(s) 9, 104, 223
Hpy188I TCNGA 1 cut(s) 565
Hpy188III TCNNGA 2 cut(s) 416, 557
Hpy8I GTNNAC 3 cut(s) 9, 104, 223
HpyAV CCTTC 1 cut(s) 340
HpyCH4III ACNGT 2 cut(s) 16, 547
HpyCH4V TGCA 2 cut(s) 609, 624
HpyF3I CTNAG 1 cut(s) 68
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 121, 555
Kzo9I GATC 5 cut(s) 109, 232, 412, 418, 638
LmnI GCTCC 2 cut(s) 326, 517
LpnPI CCDG 8 cut(s) 108, 150, 177, 187, 420, 582, 656, 750
Lsp1109I GCAGC 4 cut(s) 265, 340, 593, 621
LweI GCATC 4 cut(s) 232, 265, 340, 621
MabI ACCWGGT 1 cut(s) 163
MaeI CTAG 1 cut(s) 456
MaeIII GTNAC 4 cut(s) 211, 361, 691, 709
MalI GATC 5 cut(s) 111, 234, 414, 420, 640
MboI GATC 5 cut(s) 109, 232, 412, 418, 638
MboII GAAGA 3 cut(s) 209, 503, 677
MflI RGATCY 1 cut(s) 638
MluCI AATT 2 cut(s) 138, 273
MlyI GAGTC 1 cut(s) 706
MmeI TCCRAC 1 cut(s) 566
MnlI CCTC 8 cut(s) 63, 123, 167, 243, 486, 525, 686, 743
MseI TTAA 3 cut(s) 51, 114, 261
MspCI CTTAAG 1 cut(s) 260
MspI CCGG 1 cut(s) 643
MspR9I CCNGG 2 cut(s) 165, 643
MvaI CCWGG 1 cut(s) 165
NciI CCSGG 1 cut(s) 643
NdeII GATC 5 cut(s) 109, 232, 412, 418, 638
NlaIII CATG 2 cut(s) 121, 555
NlaIV GGNNCC 1 cut(s) 640
NmuCI GTSAC 2 cut(s) 361, 709
NspI RCATGY 1 cut(s) 555
NspV TTCGAA 1 cut(s) 78
PfeI GAWTC 3 cut(s) 431, 560, 684
PflMI CCANNNNNTGG 1 cut(s) 635
PkrI GCNGC 4 cut(s) 255, 355, 608, 611
PleI GAGTC 1 cut(s) 705
PpsI GAGTC 1 cut(s) 705
PsiI TTATAA 1 cut(s) 525
Psp6I CCWGG 1 cut(s) 163
PspGI CCWGG 1 cut(s) 163
PspN4I GGNNCC 1 cut(s) 640
PstI CTGCAG 1 cut(s) 611
PstNI CAGNNNCTG 1 cut(s) 615
PsuI RGATCY 1 cut(s) 638
RsaI GTAC 1 cut(s) 282
RsaNI GTAC 1 cut(s) 281
SaqAI TTAA 3 cut(s) 51, 114, 261
SatI GCNGC 4 cut(s) 254, 354, 607, 610
Sau3AI GATC 5 cut(s) 109, 232, 412, 418, 638
SchI GAGTC 1 cut(s) 706
ScrFI CCNGG 2 cut(s) 165, 643
SexAI ACCWGGT 1 cut(s) 163
SfaNI GCATC 4 cut(s) 232, 265, 340, 621
SfcI CTRYAG 1 cut(s) 607
SfuI TTCGAA 1 cut(s) 78
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
Sse9I AATT 2 cut(s) 138, 273
SsiI CCGC 1 cut(s) 144
SspI AATATT 1 cut(s) 314
SspMI CTAG 1 cut(s) 456
StyD4I CCNGG 2 cut(s) 163, 641
StyI CCWWGG 1 cut(s) 45
TaaI ACNGT 2 cut(s) 16, 547
TaqI TCGA 4 cut(s) 78, 192, 653, 682
TasI AATT 2 cut(s) 138, 273
TatI WGTACW 1 cut(s) 280
TfiI GAWTC 3 cut(s) 431, 560, 684
Tru1I TTAA 3 cut(s) 51, 114, 261
Tru9I TTAA 3 cut(s) 51, 114, 261
TscAI CASTG 1 cut(s) 601
TseFI GTSAC 2 cut(s) 361, 709
TseI GCWGC 4 cut(s) 253, 353, 606, 609
Tsp45I GTSAC 2 cut(s) 361, 709
TspDTI ATGAA 3 cut(s) 210, 302, 504
TspRI CASTG 1 cut(s) 601
Van91I CCANNNNNTGG 1 cut(s) 635
Vha464I CTTAAG 1 cut(s) 260
XapI RAATTY 1 cut(s) 138
XceI RCATGY 1 cut(s) 555
XspI CTAG 1 cut(s) 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.