Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0015782

Physical Location & Seq

Genomic Coordinates Forward (+)

32383347 .. 32387278

Gene Structure 3932 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1186000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1650 bp

ATGGGGAACTGCAACGTCTGCGTGAGAGCTGACGTCGTCAGCGGAGACGACAAGAGTTCCAGCGGCGATAAAAACAAGAAGAAGGCCCGGGAGCGGCGGTCCAACCCGTTTTCCGACGACCCGGCCGAATCCCCGGCTCCGATCCGAGTTCTCAAGGACGTGATTCCACTTGGCCACCGAACCCGGATCGGCGACAAGTACGTGCTAGGTCGGGAACTGGGTCGGGGCGAGTTCGGCATCACGTATCTGTGCACGGACCGCGAGACAAAGCAGGCACTCGCCTGCAAGTCCATATCGAAACGCAAGCTTCGAACGGCGGTGGATATAGAGGATGTGCGGCGGGAGGTGGCGATAATGTCGACGCTGCCGGAGCACCCGAACATAGTGAAGCTGAAAGCGACGTACGAGGACAACGAGAACGTTCATTTGGTCATGGAGCTCTGCGAGGGCGGCGAGCTTTTCGATAGGATTGTGGCCAGAGGTCATTACAGTGAGCGGGCGGCGGCGAACGTCGCCAGGACGGTGGCGGAGGTGGTGAGGATGTGCCATGCTAATGGGGTTATGCACAGGGACTTGAAGCCCGAAAACTTTCTGTTCTCGAACAAGAAGGAGCACGCTCCTCTTAAAGCCATCGACTTTGGGCTCTCTGTGTTCTTCAAGCCTGGGGAGAAGTTTATGGAGATTGTGGGTAGTCCGTACTACATGGCACCGGAGGTTTTGAAACGAAATTATGGACCGGAGGTTGATATATGGAGCGCTGGAGTGATACTTTACATTTTGTTGTGTGGGGTTCCTCCATTTTGGGCAGAGTCTGAACAGGGTGTGGCTCTTGCAATCTTGAGGGGGGTGATTGATTTCAAGAGGGAACCTTGGCCTCAGATTTCGGACAGTGCTAAAAGCCTTGTTCGGCAGATGCTGGAGCCGGATCCCAGAAAACGCTTGACCGCACAAGAGGTGCTTGAACATCCATGGCTACAAAATGCAAAGAAAGCTCCAAACGTTCCATTAGGAGATATAGTGAGGCCGAGACTCAAGCAGTTCTCAGTAATGAATAGATTCAAAAAGAAGGCCCTAAGGGTGATCGCAGAACACTTATCTATTAAAGAAGTTGAAGTAATCAAAGACATGTTTACGTTGATGGACACTGACAAAAATGGCAAAATAACATATGAGGAGTTGAAGTTGGGGCTCCGCAAAGTTGGTTCACAATTGGCTGAACCGGAGATTAAGATGTTGATGGAAGTGGCTGATGTTGATGGGAATGGCGTTCTGGACTACGGAGAGTTTGCAGCAGTAACAATTCACTTGCAGAAGCTGGAGAACGATGAGCATCTCCACAGAGCATTCGTGTTCTTTGATAAAGATGGAAGTGGATATATTGAATTAGGTGAGCTAAGGGAAGGTTTACGAGATGAATCAGGTGAAACTGATATTGAGGTGCTGAATGACATCATGCGTGAGGTCGACACTGACAAGGATGGACGCATCAGTTATGAGGAGTTTGTTGCGATGATGAAAACGGGAACTGATTGGAGAAAGGCATCTCGACAGTATTCAAGGGAGCGATTCAAGAGTTTAAGCCTCAACCTTATGAAAGATGGTTCCTTGCAGCTTCACGACGGGCTAACTGGTCAAGCCATTGCCGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009555 GO:0009628 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010152 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0021700 GO:0023052 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0048229 GO:0048518 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080134 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701 GO:1902584 GO:2000070

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 Pkinase

Protein Analysis

550

Amino Acids

62.04

Weight (kDa)

6.54

Isoelectric Point (pI)

34.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	67 - 325	3.8e-79	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	69 - 321	2.6e-27	Protein tyrosine and serine/threonine kinase
Kdo	PF06293	103 - 213	7.4e-06	Lipopolysaccharide kinase (Kdo/WaaP) family
EF-hand_7	PF13499	371 - 432	2.6e-11	EF-hand domain pair
EF-hand_1	PF00036	373 - 399	1.9e-07	EF hand domain
EF-hand_6	PF13405	373 - 401	6.3e-06	EF-hand domain
EF-hand_7	PF13499	443 - 506	5.3e-17	EF-hand domain pair
EF-hand_8	PF13833	457 - 506	8.3e-06	EF-hand domain pair
EF-hand_1	PF00036	481 - 507	9.3e-09	EF hand domain
EF-hand_5	PF13202	481 - 505	6.3e-06	EF hand

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Orthologous Genes (Group: OG0015782)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18890 AT1G74740
fragaria_vesca	FvH4_5g15720
malus_domestica	MD06G1186000.v1.1 MD14G1192200.v1.1
prunus_persica	Prupe.5G184800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0180391
rosa_laevigata	RLG00000005271
rosa_multiflora	Rmu_sc0003526.1_g000008
rosa_rugosa	Rorug06G0432800
rosa_samantha	Rh7AG033300 Rh7BG033200 Rh7CG035200 Rh7DG033700
rosa_wichuraiana	Rw7G002780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	36
AccB1I	GGYRCC	1 cut(s)	706
AccBSI	CCGCTC	2 cut(s)	94, 496
AccI	GTMKAC	2 cut(s)	359, 1464
AccII	CGCG	1 cut(s)	261
AclI	AACGTT	2 cut(s)	420, 999
AclWI	GGATC	4 cut(s)	136, 194, 920, 933
AcoI	YGGCCR	3 cut(s)	123, 172, 474
AcyI	GRCGYC	1 cut(s)	33
AfaI	GTAC	3 cut(s)	200, 404, 698
AfeI	AGCGCT	1 cut(s)	757
AfiI	CCNNNNNNNGG	1 cut(s)	93
AflIII	ACRYGT	1 cut(s)	1125
AjiI	CACGTC	1 cut(s)	160
AjnI	CCWGG	2 cut(s)	515, 661
AjuI	GAANNNNNNNTTGG	2 cut(s)	410, 442
AluBI	AGCT	9 cut(s)	29, 307, 391, 439, 457, 992, 1315, 1393, 1612
AluI	AGCT	9 cut(s)	29, 307, 391, 439, 457, 992, 1315, 1393, 1612
Alw21I	GWGCWC	4 cut(s)	254, 375, 441, 615
Alw26I	GTCTC	3 cut(s)	39, 257, 1021
Alw44I	GTGCAC	1 cut(s)	250
AlwI	GGATC	4 cut(s)	136, 194, 920, 933
AlwNI	CAGNNNCTG	3 cut(s)	812, 916, 1315
Ama87I	CYCGRG	1 cut(s)	87
Aor51HI	AGCGCT	1 cut(s)	757
AoxI	GGCC	7 cut(s)	84, 123, 172, 474, 872, 1022, 1068
ApaLI	GTGCAC	1 cut(s)	250
ApeKI	GCWGC	3 cut(s)	364, 1289, 1609
Asp700I	GAANNNNTTC	2 cut(s)	588, 1055
AspLEI	GCGC	1 cut(s)	758
AspS9I	GGNCC	5 cut(s)	85, 99, 256, 734, 1069
AsuC2I	CCSGG	5 cut(s)	88, 89, 122, 134, 184
AsuHPI	GGTGA	5 cut(s)	547, 859, 1090, 1400, 1433
AsuII	TTCGAA	1 cut(s)	310
AvaI	CYCGRG	1 cut(s)	87
AvaII	GGWCC	3 cut(s)	99, 256, 734
AxyI	CCTNAGG	1 cut(s)	1073
BaeGI	GKGCMC	1 cut(s)	254
BalI	TGGCCA	2 cut(s)	174, 476
BamHI	GGATCC	1 cut(s)	925
BanI	GGYRCC	1 cut(s)	706
BanII	GRGCYC	3 cut(s)	441, 645, 1191
Bbv12I	GWGCWC	4 cut(s)	254, 375, 441, 615
BbvI	GCAGC	3 cut(s)	351, 1301, 1621
BccI	CCATC	7 cut(s)	638, 1132, 1231, 1250, 1358, 1472, 1592
BceAI	ACGGC	2 cut(s)	330, 1628
BciT130I	CCWGG	2 cut(s)	517, 663
BcnI	CCSGG	5 cut(s)	88, 89, 122, 134, 184
BcoDI	GTCTC	3 cut(s)	39, 257, 1021
BfaI	CTAG	1 cut(s)	206
BfoI	RGCGCY	1 cut(s)	759
BisI	GCNGC	9 cut(s)	64, 95, 338, 365, 451, 501, 504, 1290, 1610
BlsI	GCNGC	9 cut(s)	65, 96, 339, 366, 452, 502, 505, 1291, 1611
Bme1390I	CCNGG	7 cut(s)	88, 89, 122, 134, 184, 517, 663
Bme18I	GGWCC	3 cut(s)	99, 256, 734
BmeT110I	CYCGRG	1 cut(s)	87
BmgBI	CACGTC	1 cut(s)	160
BmgT120I	GGNCC	5 cut(s)	85, 99, 256, 734, 1069
BmiI	GGNNCC	8 cut(s)	138, 708, 792, 867, 921, 927, 1190, 1603
BmrFI	CCNGG	7 cut(s)	88, 89, 122, 134, 184, 517, 663
BmrI	ACTGGG	1 cut(s)	227
BmsI	GCATC	5 cut(s)	246, 903, 1339, 1494, 1550
BmuI	ACTGGG	1 cut(s)	227
BpmI	CTGGAG	3 cut(s)	780, 938, 1337
Bpu10I	CCTNAGC	1 cut(s)	1394
Bpu14I	TTCGAA	1 cut(s)	310
BpuEI	CTTGAG	3 cut(s)	137, 859, 1016
BpuMI	CCSGG	5 cut(s)	88, 89, 122, 134, 184
BsaAI	YACGTR	2 cut(s)	202, 243
BsaBI	GATNNNNATC	1 cut(s)	1329
BsaHI	GRCGYC	1 cut(s)	33
BsaJI	CCNNGG	5 cut(s)	87, 132, 662, 869, 968
BsaWI	WCCGGW	3 cut(s)	709, 736, 1219
BsaXI	ACNNNNNCTCC	6 cut(s)	83, 113, 1002, 1032, 1166, 1196
Bsc4I	CCNNNNNNNGG	1 cut(s)	93
Bse1I	ACTGG	2 cut(s)	222, 1633
Bse21I	CCTNAGG	1 cut(s)	1073
Bse3DI	GCAATG	1 cut(s)	1638
Bse8I	GATNNNNATC	1 cut(s)	1329
BseBI	CCWGG	2 cut(s)	517, 663
BseDI	CCNNGG	5 cut(s)	87, 132, 662, 869, 968
BseGI	GGATG	4 cut(s)	337, 546, 964, 1483
BseJI	GATNNNNATC	1 cut(s)	1329
BseLI	CCNNNNNNNGG	1 cut(s)	93
BseMI	GCAATG	1 cut(s)	1638
BseMII	CTCAG	2 cut(s)	890, 1056
BseNI	ACTGG	2 cut(s)	222, 1633
BseRI	GAGGAG	3 cut(s)	609, 1187, 1511
BseSI	GKGCMC	1 cut(s)	254
BseX3I	CGGCCG	1 cut(s)	123
BseXI	GCAGC	3 cut(s)	351, 1301, 1621
Bsh1236I	CGCG	1 cut(s)	261
Bsh1285I	CGRYCG	1 cut(s)	126
BshFI	GGCC	7 cut(s)	86, 125, 174, 476, 874, 1024, 1070
BshNI	GGYRCC	1 cut(s)	706
BsiEI	CGRYCG	1 cut(s)	126
BsiHKAI	GWGCWC	4 cut(s)	254, 375, 441, 615
BsiHKCI	CYCGRG	1 cut(s)	87
BsiSI	CCGG	9 cut(s)	88, 122, 134, 184, 368, 710, 737, 923, 1220
BsiWI	CGTACG	1 cut(s)	402
BslFI	GGGAC	1 cut(s)	584
BslI	CCNNNNNNNGG	1 cut(s)	93
BsmAI	GTCTC	3 cut(s)	39, 257, 1021
BsmBI	CGTCTC	1 cut(s)	39
BsmFI	GGGAC	1 cut(s)	584
BsmI	GAATGC	1 cut(s)	1343
BsnI	GGCC	7 cut(s)	86, 125, 174, 476, 874, 1024, 1070
BsoBI	CYCGRG	1 cut(s)	87
Bsp119I	TTCGAA	1 cut(s)	310
Bsp1286I	GDGCHC	6 cut(s)	254, 375, 441, 615, 645, 1191
Bsp143I	GATC	4 cut(s)	141, 186, 925, 1080
Bsp19I	CCATGG	1 cut(s)	968
BspANI	GGCC	7 cut(s)	86, 125, 174, 476, 874, 1024, 1070
BspCNI	CTCAG	2 cut(s)	889, 1055
BspFNI	CGCG	1 cut(s)	261
BspLI	GGNNCC	8 cut(s)	138, 708, 792, 867, 921, 927, 1190, 1603
BspPI	GGATC	4 cut(s)	136, 194, 920, 933
BspT104I	TTCGAA	1 cut(s)	310
BspT107I	GGYRCC	1 cut(s)	706
BsrBI	CCGCTC	2 cut(s)	94, 496
BsrDI	GCAATG	1 cut(s)	1638
BsrI	ACTGG	2 cut(s)	222, 1633
BssECI	CCNNGG	5 cut(s)	87, 132, 662, 869, 968
BssMI	GATC	4 cut(s)	141, 186, 925, 1080
BssNI	GRCGYC	1 cut(s)	33
BssT1I	CCWWGG	2 cut(s)	869, 968
Bst2UI	CCWGG	2 cut(s)	517, 663
Bst4CI	ACNGT	4 cut(s)	491, 523, 890, 1551
BstACI	GRCGYC	1 cut(s)	33
BstAPI	GCANNNNNTGC	1 cut(s)	18
BstBAI	YACGTR	2 cut(s)	202, 243
BstBI	TTCGAA	1 cut(s)	310
BstC8I	GCNNGC	6 cut(s)	273, 283, 305, 455, 498, 615
BstDEI	CTNAG	4 cut(s)	876, 1042, 1073, 1394
BstDSI	CCRYGG	1 cut(s)	968
BstF5I	GGATG	4 cut(s)	337, 546, 964, 1483
BstFNI	CGCG	1 cut(s)	261
BstH2I	RGCGCY	1 cut(s)	759
BstHHI	GCGC	1 cut(s)	758
BstKTI	GATC	4 cut(s)	144, 189, 928, 1083
BstMAI	GTCTC	3 cut(s)	39, 257, 1021
BstMBI	GATC	4 cut(s)	141, 186, 925, 1080
BstMCI	CGRYCG	1 cut(s)	126
BstMWI	GCNNNNNNNGC	7 cut(s)	18, 234, 258, 370, 450, 512, 989
BstNI	CCWGG	2 cut(s)	517, 663
BstNSI	RCATGY	1 cut(s)	1129
BstSCI	CCNGG	7 cut(s)	86, 87, 120, 132, 182, 515, 661
BstSLI	GKGCMC	1 cut(s)	254
BstUI	CGCG	1 cut(s)	261
BstV1I	GCAGC	3 cut(s)	351, 1301, 1621
BstX2I	RGATCY	1 cut(s)	925
BstXI	CCANNNNNNTGG	2 cut(s)	523, 554
BstYI	RGATCY	1 cut(s)	925
BstZI	CGGCCG	1 cut(s)	123
Bsu36I	CCTNAGG	1 cut(s)	1073
BsuRI	GGCC	7 cut(s)	86, 125, 174, 476, 874, 1024, 1070
BtgI	CCRYGG	1 cut(s)	968
BtgZI	GCGATG	1 cut(s)	1523
BtrI	CACGTC	1 cut(s)	160
BtsCI	GGATG	4 cut(s)	337, 546, 964, 1483
BtsIMutI	CAGTG	4 cut(s)	496, 895, 1143, 1467
Cac8I	GCNNGC	6 cut(s)	273, 283, 305, 455, 498, 615
CaiI	CAGNNNCTG	3 cut(s)	812, 916, 1315
CfoI	GCGC	1 cut(s)	758
Cfr13I	GGNCC	5 cut(s)	85, 99, 256, 734, 1069
Cfr9I	CCCGGG	1 cut(s)	87
CpoI	CGGWCCG	1 cut(s)	256
CseI	GACGC	2 cut(s)	370, 1491
Csp6I	GTAC	3 cut(s)	199, 403, 697
CspI	CGGWCCG	1 cut(s)	256
CviAII	CATG	6 cut(s)	433, 548, 703, 969, 1126, 1453
CviQI	GTAC	3 cut(s)	199, 403, 697
DdeI	CTNAG	4 cut(s)	876, 1042, 1073, 1394
DpnI	GATC	4 cut(s)	143, 188, 927, 1082
DpnII	GATC	4 cut(s)	141, 186, 925, 1080
EaeI	YGGCCR	3 cut(s)	123, 172, 474
EagI	CGGCCG	1 cut(s)	123
EciI	GGCGGA	1 cut(s)	542
Ecl136II	GAGCTC	1 cut(s)	439
EclXI	CGGCCG	1 cut(s)	123
Eco130I	CCWWGG	2 cut(s)	869, 968
Eco24I	GRGCYC	3 cut(s)	441, 645, 1191
Eco47I	GGWCC	3 cut(s)	99, 256, 734
Eco47III	AGCGCT	1 cut(s)	757
Eco52I	CGGCCG	1 cut(s)	123
Eco53kI	GAGCTC	1 cut(s)	439
Eco81I	CCTNAGG	1 cut(s)	1073
Eco88I	CYCGRG	1 cut(s)	87
EcoICRI	GAGCTC	1 cut(s)	439
EcoO109I	RGGNCCY	1 cut(s)	1069
EcoRII	CCWGG	2 cut(s)	515, 661
EcoT14I	CCWWGG	2 cut(s)	869, 968
EcoT38I	GRGCYC	3 cut(s)	441, 645, 1191
ErhI	CCWWGG	2 cut(s)	869, 968
Esp3I	CGTCTC	1 cut(s)	39
FaeI	CATG	6 cut(s)	436, 551, 706, 972, 1129, 1456
FalI	AAGNNNNNCTT	2 cut(s)	942, 974
FaqI	GGGAC	1 cut(s)	584
FatI	CATG	6 cut(s)	432, 547, 702, 968, 1125, 1452
FauI	CCCGC	2 cut(s)	333, 489
FauNDI	CATATG	1 cut(s)	1168
FblI	GTMKAC	2 cut(s)	359, 1464
Fnu4HI	GCNGC	9 cut(s)	64, 95, 338, 365, 451, 501, 504, 1290, 1610
FokI	GGATG	4 cut(s)	344, 553, 951, 1490
FriOI	GRGCYC	3 cut(s)	441, 645, 1191
Fsp4HI	GCNGC	9 cut(s)	64, 95, 338, 365, 451, 501, 504, 1290, 1610
FspBI	CTAG	1 cut(s)	206
GlaI	GCGC	1 cut(s)	757
GluI	GCNGC	9 cut(s)	64, 95, 338, 365, 451, 501, 504, 1290, 1610
GsuI	CTGGAG	3 cut(s)	780, 938, 1337
HaeII	RGCGCY	1 cut(s)	759
HaeIII	GGCC	7 cut(s)	86, 125, 174, 476, 874, 1024, 1070
HapII	CCGG	9 cut(s)	88, 122, 134, 184, 368, 710, 737, 923, 1220
HgaI	GACGC	2 cut(s)	370, 1491
HhaI	GCGC	1 cut(s)	758
Hin1I	GRCGYC	1 cut(s)	33
Hin1II	CATG	6 cut(s)	436, 551, 706, 972, 1129, 1456
Hin6I	GCGC	1 cut(s)	756
HinP1I	GCGC	1 cut(s)	756
HincII	GTYRAC	2 cut(s)	360, 1465
HindII	GTYRAC	2 cut(s)	360, 1465
HindIII	AAGCTT	1 cut(s)	305
HinfI	GANTC	7 cut(s)	128, 163, 809, 1029, 1056, 1415, 1566
HpaII	CCGG	9 cut(s)	88, 122, 134, 184, 368, 710, 737, 923, 1220
HphI	GGTGA	5 cut(s)	547, 859, 1090, 1400, 1433
Hpy166II	GTNNAC	6 cut(s)	252, 360, 1131, 1205, 1406, 1465
Hpy188I	TCNGA	6 cut(s)	115, 141, 146, 814, 879, 886
Hpy188III	TCNNGA	8 cut(s)	212, 598, 838, 859, 1271, 1545, 1570, 1616
Hpy8I	GTNNAC	6 cut(s)	252, 360, 1131, 1205, 1406, 1465
Hpy99I	CGWCG	6 cut(s)	38, 119, 364, 403, 515, 1622
HpyAV	CCTTC	4 cut(s)	76, 601, 1060, 1394
HpyCH4III	ACNGT	4 cut(s)	491, 523, 890, 1551
HpyCH4V	TGCA	9 cut(s)	12, 252, 285, 565, 833, 983, 1289, 1309, 1609
HpyF10VI	GCNNNNNNNGC	7 cut(s)	18, 234, 258, 370, 450, 512, 989
HpyF3I	CTNAG	4 cut(s)	876, 1042, 1073, 1394
Hsp92I	GRCGYC	1 cut(s)	33
Hsp92II	CATG	6 cut(s)	436, 551, 706, 972, 1129, 1456
HspAI	GCGC	1 cut(s)	756
Kzo9I	GATC	4 cut(s)	141, 186, 925, 1080
Lsp1109I	GCAGC	3 cut(s)	351, 1301, 1621
LweI	GCATC	5 cut(s)	246, 903, 1339, 1494, 1550
MaeI	CTAG	1 cut(s)	206
MaeIII	GTNAC	1 cut(s)	1294
MalI	GATC	4 cut(s)	143, 188, 927, 1082
MbiI	CCGCTC	2 cut(s)	94, 496
MboI	GATC	4 cut(s)	141, 186, 925, 1080
MboII	GAAGA	2 cut(s)	91, 646
MfeI	CAATTG	1 cut(s)	1208
MflI	RGATCY	1 cut(s)	925
MhlI	GDGCHC	6 cut(s)	254, 375, 441, 615, 645, 1191
MlsI	TGGCCA	2 cut(s)	174, 476
MluCI	AATT	4 cut(s)	727, 1208, 1299, 1382
MluNI	TGGCCA	2 cut(s)	174, 476
MlyI	GAGTC	2 cut(s)	818, 1023
MmeI	TCCRAC	2 cut(s)	126, 138
Mox20I	TGGCCA	2 cut(s)	174, 476
MroXI	GAANNNNTTC	2 cut(s)	588, 1055
MscI	TGGCCA	2 cut(s)	174, 476
MseI	TTAA	4 cut(s)	624, 1101, 1227, 1577
MslI	CAYNNNNRTG	2 cut(s)	489, 552
Msp20I	TGGCCA	2 cut(s)	174, 476
MspA1I	CMGCKG	2 cut(s)	42, 63
MspI	CCGG	9 cut(s)	88, 122, 134, 184, 368, 710, 737, 923, 1220
MspR9I	CCNGG	7 cut(s)	88, 89, 122, 134, 184, 517, 663
MunI	CAATTG	1 cut(s)	1208
Mva1269I	GAATGC	1 cut(s)	1343
MvaI	CCWGG	2 cut(s)	517, 663
MvnI	CGCG	1 cut(s)	261
MwoI	GCNNNNNNNGC	7 cut(s)	18, 234, 258, 370, 450, 512, 989
NciI	CCSGG	5 cut(s)	88, 89, 122, 134, 184
NcoI	CCATGG	1 cut(s)	968
NdeI	CATATG	1 cut(s)	1168
NdeII	GATC	4 cut(s)	141, 186, 925, 1080
NlaIII	CATG	6 cut(s)	436, 551, 706, 972, 1129, 1456
NlaIV	GGNNCC	8 cut(s)	138, 708, 792, 867, 921, 927, 1190, 1603
NmeAIII	GCCGAG	1 cut(s)	1050
NspI	RCATGY	1 cut(s)	1129
NspV	TTCGAA	1 cut(s)	310
PciI	ACATGT	1 cut(s)	1125
PcsI	WCGNNNNNNNCGW	3 cut(s)	123, 307, 411
PctI	GAATGC	1 cut(s)	1343
PdmI	GAANNNNTTC	2 cut(s)	588, 1055
PfeI	GAWTC	5 cut(s)	128, 163, 1056, 1415, 1566
Pfl23II	CGTACG	1 cut(s)	402
PflFI	GACNNNGTC	1 cut(s)	35
PkrI	GCNGC	9 cut(s)	65, 96, 339, 366, 452, 502, 505, 1291, 1611
PleI	GAGTC	2 cut(s)	817, 1023
PpsI	GAGTC	2 cut(s)	817, 1023
Ppu21I	YACGTR	2 cut(s)	202, 243
PscI	ACATGT	1 cut(s)	1125
Psp124BI	GAGCTC	1 cut(s)	441
Psp1406I	AACGTT	2 cut(s)	420, 999
Psp6I	CCWGG	2 cut(s)	515, 661
PspGI	CCWGG	2 cut(s)	515, 661
PspLI	CGTACG	1 cut(s)	402
PspN4I	GGNNCC	8 cut(s)	138, 708, 792, 867, 921, 927, 1190, 1603
PspPI	GGNCC	5 cut(s)	85, 99, 256, 734, 1069
PstNI	CAGNNNCTG	3 cut(s)	812, 916, 1315
PsuI	RGATCY	1 cut(s)	925
PsyI	GACNNNGTC	1 cut(s)	35
RsaI	GTAC	3 cut(s)	200, 404, 698
RsaNI	GTAC	3 cut(s)	199, 403, 697
RseI	CAYNNNNRTG	2 cut(s)	489, 552
Rsr2I	CGGWCCG	1 cut(s)	256
RsrII	CGGWCCG	1 cut(s)	256
SacI	GAGCTC	1 cut(s)	441
SalI	GTCGAC	2 cut(s)	358, 1463
SaqAI	TTAA	4 cut(s)	624, 1101, 1227, 1577
SatI	GCNGC	9 cut(s)	64, 95, 338, 365, 451, 501, 504, 1290, 1610
Sau3AI	GATC	4 cut(s)	141, 186, 925, 1080
Sau96I	GGNCC	5 cut(s)	85, 99, 256, 734, 1069
SchI	GAGTC	2 cut(s)	818, 1023
ScrFI	CCNGG	7 cut(s)	88, 89, 122, 134, 184, 517, 663
SduI	GDGCHC	6 cut(s)	254, 375, 441, 615, 645, 1191
SfaNI	GCATC	5 cut(s)	246, 903, 1339, 1494, 1550
SfuI	TTCGAA	1 cut(s)	310
SinI	GGWCC	3 cut(s)	99, 256, 734
SmaI	CCCGGG	1 cut(s)	89
SmiMI	CAYNNNNRTG	2 cut(s)	489, 552
SmlI	CTYRAG	3 cut(s)	152, 838, 1031
SmoI	CTYRAG	3 cut(s)	152, 838, 1031
Sse9I	AATT	4 cut(s)	727, 1208, 1299, 1382
SspMI	CTAG	1 cut(s)	206
SstI	GAGCTC	1 cut(s)	441
StyD4I	CCNGG	7 cut(s)	86, 87, 120, 132, 182, 515, 661
StyI	CCWWGG	2 cut(s)	869, 968
TaaI	ACNGT	4 cut(s)	491, 523, 890, 1551
TaqI	TCGA	8 cut(s)	296, 310, 359, 462, 599, 633, 1464, 1546
TasI	AATT	4 cut(s)	727, 1208, 1299, 1382
TauI	GCSGC	6 cut(s)	66, 97, 340, 453, 503, 506
TfiI	GAWTC	5 cut(s)	128, 163, 1056, 1415, 1566
Tru1I	TTAA	4 cut(s)	624, 1101, 1227, 1577
Tru9I	TTAA	4 cut(s)	624, 1101, 1227, 1577
TscAI	CASTG	4 cut(s)	496, 895, 1150, 1474
TseI	GCWGC	3 cut(s)	364, 1289, 1609
TspDTI	ATGAA	5 cut(s)	413, 1064, 1428, 1529, 1607
TspGWI	ACGGA	3 cut(s)	269, 684, 1293
TspMI	CCCGGG	1 cut(s)	87
TspRI	CASTG	4 cut(s)	496, 895, 1150, 1474
Tth111I	GACNNNGTC	1 cut(s)	35
VneI	GTGCAC	1 cut(s)	250
VpaK11BI	GGWCC	3 cut(s)	99, 256, 734
XceI	RCATGY	1 cut(s)	1129
XmaI	CCCGGG	1 cut(s)	87
XmiI	GTMKAC	2 cut(s)	359, 1464
XmnI	GAANNNNTTC	2 cut(s)	588, 1055
XspI	CTAG	1 cut(s)	206
ZraI	GACGTC	1 cut(s)	34

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.