Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0015782

Physical Location & Seq

Genomic Coordinates Forward (+)

28311177 .. 28315400

Gene Structure 4224 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1192200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1647 bp

ATGGGGAACTGCAACGTCTGCGTGAGAGCTGACGTCATAAGTAGCGACGACAAGAATTCCGGCGATAAACAAAAGAAGAAGGCACGGGAGCGGCGGTCCAACCCGTTCTCCGACGACCCGATCCGGTCCCCGGCTCCGATCCGAGTTCTCAAGGATGTGATCCCGCTTGGCCACCGGACCCGGATCGGCGACAAGTACGTGCTGGGTCGGGAACTGGGTCGGGGCGAGTTCGGCATCACGTACCTGTGCACGGACCGGGAGACAAAGCAGGCACTCGCCTGCAAGTCCATATCGAAACGCAAGCTTCGAACGGCGGTGGATATAGAGGATGTGCGGCGGGAGGTGGCGATAATGTCGACGCTGCCGGAGCACCCGAACATTGTGAAGCTGAAGGCGACGTACGAGGACAACGAGAACGTCCATTTGGTCATGGAGCTCTGCGAGGGCGGCGAGCTTTTCGATAGGATTGTGGCCAGAGGGCATTACAGTGAGCGGGCGGCGGCGAACGTCGCCAGGACGGTGGCAGAGGTGGTGAGGATGTGCCATGCTAATGGGGTTATGCACAGGGACTTGAAGCCTGAGAACTTTCTCTTCTCGAACAAGAAGGAGCACTCGCCCCTTAAAGCCATTGACTTTGGGCTCTCTGTGTTCTTCAAGCCTGGGGAGAGGTTTATGGAGATTGTGGGCAGTCCGTACTACATGGCACCGGAGGTTTTGAAACGAAATTATGGGCCGGAGGTTGATATATGGAGCGCCGGAGTGATACTTTACATTTTGTTATGTGGAGTTCCTCCATTTTGGGCAGAGTCTGAACAGGGTGTGGCTCTTGCAATCTTGAGGGGGGTGATTGATTTCAAGAGAGAACCCTGGCCTCAGATTTCAGAGAGTGCTAAAAGCCTTGTTCGACAGATGCTGGAGCCAGATCCCAGAAAGCGCTTGACTGCGCAACAGGTGCTTGAACATCCATGGCTACATAATGCGAAGAAAGCTCCAAATGTTCCATTAGGTGATATAGTGAGGCCAAGACTCAAGCAGTTCTCAGTAATGAATAGATTCAAAAAGAAGGCCCTAAGGGTAATTGCAGAACACTTATCTGTTAAAGAAGTGGAAGTAATCAGAGACATGTTTACATTGATGGACACTGACAAAGATGGCAGAGTAACATATGAGGAATTGAAGTTGGGGCTTCACAAAGTTGGTTCACAATTGGCTGAACCGGAGATTAAGATGTTGATGGAAGTGGCTGATGTTGACGGGAATGGCGTTCTGGACTATGGAGAGTTTGCAGCAGTAACAATTCACTTGCAGAAGATGGAGAACGATGAGCATCTCCGCAGAGCGTTTGTGTTCTTTGACAAAGATGGAAGTGGATATATTGAATTAGGTGAGCTAAGGGAAAGTTTACAAGATGAATCTGGTGAAACTGATTCTGAGGTGCTGAATGACATCATGCGTGAGGTCGACACTGACAAGGATGGACGCATCAGTTATGACGAGTTTGTTGCGATGATGAAAACGGGAACCGATTGGAGAAAGGCATCTCGACAGTATTCGAGGGAGAGATTCAAGAGTTTAAGCCTCAACCTAATGAAAGATGGTTCGTTGCAGCTTCACGACGGGCTAACTGGTCAAGCCATTGCCGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009555 GO:0009628 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010152 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0021700 GO:0023052 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0048229 GO:0048518 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080134 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701 GO:1902584 GO:2000070

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 Pkinase

Protein Analysis

549

Amino Acids

62.19

Weight (kDa)

6.73

Isoelectric Point (pI)

38.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	66 - 324	3.2e-79	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	68 - 320	3.4e-27	Protein tyrosine and serine/threonine kinase
EF-hand_7	PF13499	370 - 431	8.5e-11	EF-hand domain pair
EF-hand_1	PF00036	372 - 398	2e-06	EF hand domain
EF-hand_7	PF13499	442 - 505	5.8e-18	EF-hand domain pair
EF-hand_1	PF00036	480 - 506	1.2e-08	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0015782)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18890 AT1G74740
fragaria_vesca	FvH4_5g15720
malus_domestica	MD06G1186000.v1.1 MD14G1192200.v1.1
prunus_persica	Prupe.5G184800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0180391
rosa_laevigata	RLG00000005271
rosa_multiflora	Rmu_sc0003526.1_g000008
rosa_rugosa	Rorug06G0432800
rosa_samantha	Rh7AG033300 Rh7BG033200 Rh7CG035200 Rh7DG033700
rosa_wichuraiana	Rw7G002780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	36
Acc16I	TGCGCA	1 cut(s)	945
AccB1I	GGYRCC	1 cut(s)	703
AccBSI	CCGCTC	2 cut(s)	91, 493
AccI	GTMKAC	2 cut(s)	356, 1461
AclWI	GGATC	5 cut(s)	115, 133, 154, 191, 917
AcoI	YGGCCR	2 cut(s)	169, 471
AcsI	RAATTY	1 cut(s)	55
AcuI	CTGAAG	1 cut(s)	410
AcyI	GRCGYC	1 cut(s)	33
AfaI	GTAC	4 cut(s)	197, 242, 401, 695
AfeI	AGCGCT	1 cut(s)	935
AfiI	CCNNNNNNNGG	2 cut(s)	130, 250
AflIII	ACRYGT	1 cut(s)	1122
AgsI	TTSAA	9 cut(s)	574, 655, 718, 856, 959, 1057, 1177, 1379, 1567
AjnI	CCWGG	3 cut(s)	512, 658, 866
AjuI	GAANNNNNNNTTGG	2 cut(s)	407, 439
AluBI	AGCT	8 cut(s)	29, 304, 388, 436, 454, 989, 1390, 1609
AluI	AGCT	8 cut(s)	29, 304, 388, 436, 454, 989, 1390, 1609
Alw21I	GWGCWC	4 cut(s)	251, 372, 438, 612
Alw26I	GTCTC	2 cut(s)	254, 1113
Alw44I	GTGCAC	1 cut(s)	247
AlwI	GGATC	5 cut(s)	115, 133, 154, 191, 917
AlwNI	CAGNNNCTG	2 cut(s)	809, 913
Aor51HI	AGCGCT	1 cut(s)	935
AoxI	GGCC	6 cut(s)	169, 471, 731, 869, 1019, 1065
ApaLI	GTGCAC	1 cut(s)	247
ApeKI	GCWGC	3 cut(s)	361, 1286, 1606
ApoI	RAATTY	1 cut(s)	55
Asp700I	GAANNNNTTC	1 cut(s)	1052
AspLEI	GCGC	3 cut(s)	755, 936, 946
AspS9I	GGNCC	6 cut(s)	96, 126, 177, 253, 731, 1066
AsuC2I	CCSGG	3 cut(s)	131, 181, 257
AsuHPI	GGTGA	5 cut(s)	544, 856, 1019, 1397, 1430
AsuII	TTCGAA	1 cut(s)	307
AvaII	GGWCC	4 cut(s)	96, 126, 177, 253
AxyI	CCTNAGG	1 cut(s)	1070
BaeGI	GKGCMC	1 cut(s)	251
BalI	TGGCCA	2 cut(s)	171, 473
BanI	GGYRCC	1 cut(s)	703
BanII	GRGCYC	2 cut(s)	438, 642
BarI	GAAGNNNNNNTAC	2 cut(s)	383, 415
Bbv12I	GWGCWC	4 cut(s)	251, 372, 438, 612
BbvI	GCAGC	3 cut(s)	348, 1298, 1618
BccI	CCATC	7 cut(s)	1129, 1145, 1228, 1306, 1355, 1469, 1589
BceAI	ACGGC	2 cut(s)	327, 1625
BcgI	CGANNNNNNTGC	2 cut(s)	1484, 1518
BciT130I	CCWGG	3 cut(s)	514, 660, 868
BcnI	CCSGG	3 cut(s)	131, 181, 257
BcoDI	GTCTC	2 cut(s)	254, 1113
BfoI	RGCGCY	2 cut(s)	756, 937
BisI	GCNGC	8 cut(s)	92, 335, 362, 448, 498, 501, 1287, 1607
BlsI	GCNGC	8 cut(s)	93, 336, 363, 449, 499, 502, 1288, 1608
Bme1390I	CCNGG	6 cut(s)	131, 181, 257, 514, 660, 868
Bme18I	GGWCC	4 cut(s)	96, 126, 177, 253
BmgT120I	GGNCC	6 cut(s)	96, 126, 177, 253, 731, 1066
BmiI	GGNNCC	6 cut(s)	128, 135, 179, 705, 918, 1522
BmrFI	CCNGG	6 cut(s)	131, 181, 257, 514, 660, 868
BmrI	ACTGGG	1 cut(s)	224
BmsI	GCATC	5 cut(s)	243, 900, 1336, 1491, 1547
BmuI	ACTGGG	1 cut(s)	224
BpmI	CTGGAG	1 cut(s)	935
Bpu10I	CCTNAGC	1 cut(s)	1391
Bpu14I	TTCGAA	1 cut(s)	307
BpuEI	CTTGAG	3 cut(s)	134, 856, 1013
BpuMI	CCSGG	3 cut(s)	131, 181, 257
BsaAI	YACGTR	2 cut(s)	199, 240
BsaBI	GATNNNNATC	1 cut(s)	1326
BsaHI	GRCGYC	1 cut(s)	33
BsaJI	CCNNGG	4 cut(s)	129, 659, 866, 965
BsaWI	WCCGGW	4 cut(s)	123, 174, 706, 1216
BsaXI	ACNNNNNCTCC	4 cut(s)	80, 92, 110, 122
Bsc4I	CCNNNNNNNGG	2 cut(s)	130, 250
Bse1I	ACTGG	2 cut(s)	219, 1630
Bse21I	CCTNAGG	1 cut(s)	1070
Bse3DI	GCAATG	1 cut(s)	1635
Bse8I	GATNNNNATC	1 cut(s)	1326
BseBI	CCWGG	3 cut(s)	514, 660, 868
BseDI	CCNNGG	4 cut(s)	129, 659, 866, 965
BseGI	GGATG	5 cut(s)	160, 334, 543, 961, 1480
BseJI	GATNNNNATC	1 cut(s)	1326
BseLI	CCNNNNNNNGG	2 cut(s)	130, 250
BseMI	GCAATG	1 cut(s)	1635
BseMII	CTCAG	4 cut(s)	570, 887, 1053, 1422
BseNI	ACTGG	2 cut(s)	219, 1630
BseSI	GKGCMC	1 cut(s)	251
BseXI	GCAGC	3 cut(s)	348, 1298, 1618
BseYI	CCCAGC	1 cut(s)	202
BshFI	GGCC	6 cut(s)	171, 473, 733, 871, 1021, 1067
BshNI	GGYRCC	1 cut(s)	703
BsiHKAI	GWGCWC	4 cut(s)	251, 372, 438, 612
BsiWI	CGTACG	1 cut(s)	399
BslFI	GGGAC	2 cut(s)	112, 581
BslI	CCNNNNNNNGG	2 cut(s)	130, 250
BsmAI	GTCTC	2 cut(s)	254, 1113
BsmFI	GGGAC	2 cut(s)	112, 581
BsnI	GGCC	6 cut(s)	171, 473, 733, 871, 1021, 1067
Bsp119I	TTCGAA	1 cut(s)	307
Bsp1286I	GDGCHC	5 cut(s)	251, 372, 438, 612, 642
Bsp143I	GATC	5 cut(s)	120, 138, 159, 183, 922
Bsp19I	CCATGG	1 cut(s)	965
BspANI	GGCC	6 cut(s)	171, 473, 733, 871, 1021, 1067
BspCNI	CTCAG	4 cut(s)	571, 886, 1052, 1423
BspLI	GGNNCC	6 cut(s)	128, 135, 179, 705, 918, 1522
BspPI	GGATC	5 cut(s)	115, 133, 154, 191, 917
BspT104I	TTCGAA	1 cut(s)	307
BspT107I	GGYRCC	1 cut(s)	703
BsrBI	CCGCTC	2 cut(s)	91, 493
BsrDI	GCAATG	1 cut(s)	1635
BsrI	ACTGG	2 cut(s)	219, 1630
BssECI	CCNNGG	4 cut(s)	129, 659, 866, 965
BssMI	GATC	5 cut(s)	120, 138, 159, 183, 922
BssNI	GRCGYC	1 cut(s)	33
BssT1I	CCWWGG	1 cut(s)	965
Bst2UI	CCWGG	3 cut(s)	514, 660, 868
Bst4CI	ACNGT	3 cut(s)	488, 520, 1548
Bst6I	CTCTTC	1 cut(s)	596
BstACI	GRCGYC	1 cut(s)	33
BstAPI	GCANNNNNTGC	2 cut(s)	18, 952
BstBAI	YACGTR	2 cut(s)	199, 240
BstBI	TTCGAA	1 cut(s)	307
BstC8I	GCNNGC	5 cut(s)	270, 280, 302, 452, 495
BstDEI	CTNAG	6 cut(s)	579, 873, 1039, 1070, 1391, 1431
BstDSI	CCRYGG	1 cut(s)	965
BstF5I	GGATG	5 cut(s)	160, 334, 543, 961, 1480
BstH2I	RGCGCY	2 cut(s)	756, 937
BstHHI	GCGC	3 cut(s)	755, 936, 946
BstKTI	GATC	5 cut(s)	123, 141, 162, 186, 925
BstMAI	GTCTC	2 cut(s)	254, 1113
BstMBI	GATC	5 cut(s)	120, 138, 159, 183, 922
BstMWI	GCNNNNNNNGC	7 cut(s)	18, 231, 367, 447, 509, 952, 986
BstNI	CCWGG	3 cut(s)	514, 660, 868
BstNSI	RCATGY	1 cut(s)	1126
BstSCI	CCNGG	6 cut(s)	129, 179, 255, 512, 658, 866
BstSLI	GKGCMC	1 cut(s)	251
BstV1I	GCAGC	3 cut(s)	348, 1298, 1618
BstX2I	RGATCY	1 cut(s)	922
BstXI	CCANNNNNNTGG	2 cut(s)	520, 551
BstYI	RGATCY	1 cut(s)	922
Bsu36I	CCTNAGG	1 cut(s)	1070
BsuRI	GGCC	6 cut(s)	171, 473, 733, 871, 1021, 1067
BtgI	CCRYGG	1 cut(s)	965
BtgZI	GCGATG	1 cut(s)	1520
BtsCI	GGATG	5 cut(s)	160, 334, 543, 961, 1480
BtsIMutI	CAGTG	3 cut(s)	493, 1140, 1464
Cac8I	GCNNGC	5 cut(s)	270, 280, 302, 452, 495
CaiI	CAGNNNCTG	2 cut(s)	809, 913
CfoI	GCGC	3 cut(s)	755, 936, 946
Cfr13I	GGNCC	6 cut(s)	96, 126, 177, 253, 731, 1066
CpoI	CGGWCCG	1 cut(s)	253
CseI	GACGC	2 cut(s)	367, 1488
Csp6I	GTAC	4 cut(s)	196, 241, 400, 694
CspI	CGGWCCG	1 cut(s)	253
CviAII	CATG	6 cut(s)	430, 545, 700, 966, 1123, 1450
CviQI	GTAC	4 cut(s)	196, 241, 400, 694
DdeI	CTNAG	6 cut(s)	579, 873, 1039, 1070, 1391, 1431
DpnI	GATC	5 cut(s)	122, 140, 161, 185, 924
DpnII	GATC	5 cut(s)	120, 138, 159, 183, 922
EaeI	YGGCCR	2 cut(s)	169, 471
Eam1104I	CTCTTC	1 cut(s)	596
EarI	CTCTTC	1 cut(s)	596
Ecl136II	GAGCTC	1 cut(s)	436
Eco130I	CCWWGG	1 cut(s)	965
Eco24I	GRGCYC	2 cut(s)	438, 642
Eco47I	GGWCC	4 cut(s)	96, 126, 177, 253
Eco47III	AGCGCT	1 cut(s)	935
Eco53kI	GAGCTC	1 cut(s)	436
Eco57I	CTGAAG	1 cut(s)	410
Eco81I	CCTNAGG	1 cut(s)	1070
EcoICRI	GAGCTC	1 cut(s)	436
EcoO109I	RGGNCCY	1 cut(s)	1066
EcoRI	GAATTC	1 cut(s)	55
EcoRII	CCWGG	3 cut(s)	512, 658, 866
EcoT14I	CCWWGG	1 cut(s)	965
EcoT38I	GRGCYC	2 cut(s)	438, 642
ErhI	CCWWGG	1 cut(s)	965
FaeI	CATG	6 cut(s)	433, 548, 703, 969, 1126, 1453
FaqI	GGGAC	2 cut(s)	112, 581
FatI	CATG	6 cut(s)	429, 544, 699, 965, 1122, 1449
FauI	CCCGC	3 cut(s)	171, 330, 486
FauNDI	CATATG	1 cut(s)	1165
FblI	GTMKAC	2 cut(s)	356, 1461
Fnu4HI	GCNGC	8 cut(s)	92, 335, 362, 448, 498, 501, 1287, 1607
FokI	GGATG	5 cut(s)	167, 341, 550, 948, 1487
FriOI	GRGCYC	2 cut(s)	438, 642
Fsp4HI	GCNGC	8 cut(s)	92, 335, 362, 448, 498, 501, 1287, 1607
FspI	TGCGCA	1 cut(s)	945
GlaI	GCGC	3 cut(s)	754, 935, 945
GluI	GCNGC	8 cut(s)	92, 335, 362, 448, 498, 501, 1287, 1607
GsaI	CCCAGC	1 cut(s)	206
GsuI	CTGGAG	1 cut(s)	935
HaeII	RGCGCY	2 cut(s)	756, 937
HaeIII	GGCC	6 cut(s)	171, 473, 733, 871, 1021, 1067
HgaI	GACGC	2 cut(s)	367, 1488
HhaI	GCGC	3 cut(s)	755, 936, 946
Hin1I	GRCGYC	1 cut(s)	33
Hin1II	CATG	6 cut(s)	433, 548, 703, 969, 1126, 1453
Hin6I	GCGC	3 cut(s)	753, 934, 944
HinP1I	GCGC	3 cut(s)	753, 934, 944
HincII	GTYRAC	3 cut(s)	357, 1252, 1462
HindII	GTYRAC	3 cut(s)	357, 1252, 1462
HindIII	AAGCTT	1 cut(s)	302
HinfI	GANTC	6 cut(s)	806, 1026, 1053, 1412, 1427, 1563
HphI	GGTGA	5 cut(s)	544, 856, 1019, 1397, 1430
Hpy166II	GTNNAC	7 cut(s)	249, 357, 1128, 1202, 1252, 1403, 1462
Hpy188I	TCNGA	8 cut(s)	112, 138, 143, 811, 876, 883, 1118, 1432
Hpy188III	TCNNGA	8 cut(s)	209, 595, 835, 856, 1268, 1542, 1567, 1613
Hpy8I	GTNNAC	7 cut(s)	249, 357, 1128, 1202, 1252, 1403, 1462
Hpy99I	CGWCG	6 cut(s)	50, 116, 361, 400, 512, 1619
HpyAV	CCTTC	4 cut(s)	73, 385, 598, 1057
HpyCH4III	ACNGT	3 cut(s)	488, 520, 1548
HpyCH4IV	ACGT	7 cut(s)	15, 33, 198, 239, 398, 417, 507
HpyCH4V	TGCA	9 cut(s)	12, 249, 282, 562, 830, 1082, 1286, 1306, 1606
HpyF10VI	GCNNNNNNNGC	7 cut(s)	18, 231, 367, 447, 509, 952, 986
HpyF3I	CTNAG	6 cut(s)	579, 873, 1039, 1070, 1391, 1431
HpySE526I	ACGT	7 cut(s)	15, 33, 198, 239, 398, 417, 507
Hsp92I	GRCGYC	1 cut(s)	33
Hsp92II	CATG	6 cut(s)	433, 548, 703, 969, 1126, 1453
HspAI	GCGC	3 cut(s)	753, 934, 944
Kzo9I	GATC	5 cut(s)	120, 138, 159, 183, 922
LmnI	GCTCC	8 cut(s)	88, 139, 367, 433, 607, 750, 916, 994
Lsp1109I	GCAGC	3 cut(s)	348, 1298, 1618
LweI	GCATC	5 cut(s)	243, 900, 1336, 1491, 1547
MaeII	ACGT	7 cut(s)	15, 33, 198, 239, 398, 417, 507
MaeIII	GTNAC	2 cut(s)	1159, 1291
MalI	GATC	5 cut(s)	122, 140, 161, 185, 924
MbiI	CCGCTC	2 cut(s)	91, 493
MboI	GATC	5 cut(s)	120, 138, 159, 183, 922
MboII	GAAGA	5 cut(s)	88, 583, 643, 994, 1321
MfeI	CAATTG	1 cut(s)	1205
MflI	RGATCY	1 cut(s)	922
MhlI	GDGCHC	5 cut(s)	251, 372, 438, 612, 642
MlsI	TGGCCA	2 cut(s)	171, 473
MluCI	AATT	7 cut(s)	55, 724, 1077, 1172, 1205, 1296, 1379
MluNI	TGGCCA	2 cut(s)	171, 473
MlyI	GAGTC	2 cut(s)	815, 1020
MmeI	TCCRAC	2 cut(s)	123, 135
Mox20I	TGGCCA	2 cut(s)	171, 473
MroXI	GAANNNNTTC	1 cut(s)	1052
MscI	TGGCCA	2 cut(s)	171, 473
MseI	TTAA	4 cut(s)	621, 1098, 1224, 1574
MslI	CAYNNNNRTG	2 cut(s)	486, 549
Msp20I	TGGCCA	2 cut(s)	171, 473
MspR9I	CCNGG	6 cut(s)	131, 181, 257, 514, 660, 868
MunI	CAATTG	1 cut(s)	1205
MvaI	CCWGG	3 cut(s)	514, 660, 868
MwoI	GCNNNNNNNGC	7 cut(s)	18, 231, 367, 447, 509, 952, 986
NciI	CCSGG	3 cut(s)	131, 181, 257
NcoI	CCATGG	1 cut(s)	965
NdeI	CATATG	1 cut(s)	1165
NdeII	GATC	5 cut(s)	120, 138, 159, 183, 922
NlaIII	CATG	6 cut(s)	433, 548, 703, 969, 1126, 1453
NlaIV	GGNNCC	6 cut(s)	128, 135, 179, 705, 918, 1522
NsbI	TGCGCA	1 cut(s)	945
NspI	RCATGY	1 cut(s)	1126
NspV	TTCGAA	1 cut(s)	307
PciI	ACATGT	1 cut(s)	1122
PcsI	WCGNNNNNNNCGW	3 cut(s)	304, 408, 1260
PdmI	GAANNNNTTC	1 cut(s)	1052
PfeI	GAWTC	4 cut(s)	1053, 1412, 1427, 1563
Pfl23II	CGTACG	1 cut(s)	399
PkrI	GCNGC	8 cut(s)	93, 336, 363, 449, 499, 502, 1288, 1608
PleI	GAGTC	2 cut(s)	814, 1020
PpsI	GAGTC	2 cut(s)	814, 1020
Ppu21I	YACGTR	2 cut(s)	199, 240
PscI	ACATGT	1 cut(s)	1122
Psp124BI	GAGCTC	1 cut(s)	438
Psp6I	CCWGG	3 cut(s)	512, 658, 866
PspFI	CCCAGC	1 cut(s)	202
PspGI	CCWGG	3 cut(s)	512, 658, 866
PspLI	CGTACG	1 cut(s)	399
PspN4I	GGNNCC	6 cut(s)	128, 135, 179, 705, 918, 1522
PspPI	GGNCC	6 cut(s)	96, 126, 177, 253, 731, 1066
PstNI	CAGNNNCTG	2 cut(s)	809, 913
PsuI	RGATCY	1 cut(s)	922
RsaI	GTAC	4 cut(s)	197, 242, 401, 695
RsaNI	GTAC	4 cut(s)	196, 241, 400, 694
RseI	CAYNNNNRTG	2 cut(s)	486, 549
Rsr2I	CGGWCCG	1 cut(s)	253
RsrII	CGGWCCG	1 cut(s)	253
SacI	GAGCTC	1 cut(s)	438
SalI	GTCGAC	2 cut(s)	355, 1460
SaqAI	TTAA	4 cut(s)	621, 1098, 1224, 1574
SatI	GCNGC	8 cut(s)	92, 335, 362, 448, 498, 501, 1287, 1607
Sau3AI	GATC	5 cut(s)	120, 138, 159, 183, 922
Sau96I	GGNCC	6 cut(s)	96, 126, 177, 253, 731, 1066
SchI	GAGTC	2 cut(s)	815, 1020
ScrFI	CCNGG	6 cut(s)	131, 181, 257, 514, 660, 868
SduI	GDGCHC	5 cut(s)	251, 372, 438, 612, 642
SfaNI	GCATC	5 cut(s)	243, 900, 1336, 1491, 1547
SfuI	TTCGAA	1 cut(s)	307
SinI	GGWCC	4 cut(s)	96, 126, 177, 253
SmiMI	CAYNNNNRTG	2 cut(s)	486, 549
SmlI	CTYRAG	3 cut(s)	149, 835, 1028
SmoI	CTYRAG	3 cut(s)	149, 835, 1028
Sse9I	AATT	7 cut(s)	55, 724, 1077, 1172, 1205, 1296, 1379
SstI	GAGCTC	1 cut(s)	438
StyD4I	CCNGG	6 cut(s)	129, 179, 255, 512, 658, 866
StyI	CCWWGG	1 cut(s)	965
TaaI	ACNGT	3 cut(s)	488, 520, 1548
TaiI	ACGT	7 cut(s)	18, 36, 201, 242, 401, 420, 510
TaqI	TCGA	9 cut(s)	293, 307, 356, 459, 596, 904, 1461, 1543, 1553
TasI	AATT	7 cut(s)	55, 724, 1077, 1172, 1205, 1296, 1379
TauI	GCSGC	5 cut(s)	94, 337, 450, 500, 503
TfiI	GAWTC	4 cut(s)	1053, 1412, 1427, 1563
Tru1I	TTAA	4 cut(s)	621, 1098, 1224, 1574
Tru9I	TTAA	4 cut(s)	621, 1098, 1224, 1574
TscAI	CASTG	3 cut(s)	493, 1147, 1471
TseI	GCWGC	3 cut(s)	361, 1286, 1606
TspDTI	ATGAA	4 cut(s)	1061, 1425, 1526, 1604
TspGWI	ACGGA	2 cut(s)	266, 681
TspRI	CASTG	3 cut(s)	493, 1147, 1471
VneI	GTGCAC	1 cut(s)	247
VpaK11BI	GGWCC	4 cut(s)	96, 126, 177, 253
XapI	RAATTY	1 cut(s)	55
XceI	RCATGY	1 cut(s)	1126
XmiI	GTMKAC	2 cut(s)	356, 1461
XmnI	GAANNNNTTC	1 cut(s)	1052
ZraI	GACGTC	1 cut(s)	34

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.