Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7D

Orthogroup ID

OG0015782

Physical Location & Seq

Genomic Coordinates Forward (+)

2469385 .. 2473843

Gene Structure 4459 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7DG033700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1479 bp

ATGGGGAACTGCAACGTGTGCGTCAGAGCTGACGTCATCGAGAGCCAGGGCACCTACCAGGCGAACAACAAGAAGAAGAAGGCCGGGGAGCGGCGGCCGAACCCGTTCTCCGAGGACGCGATCCGGTCGCCGGCGCCGATCCGAGTGCTCAAGGACGTGATTCCGCTCGGTCACCGGACTCGGATCGGCGACAAGTACATACTGGGCCGGGAGCTGGGCCGCGGGGAGTTCGGCATCACCTATCTCTGTACGGACCGCGAGACTAAAGAAGCTCTGGCCTGCAAGTCCATCTCCAAACGGAAGCTCCGGACTGCTGTGGATATAGAGGACGTGAGGCGCGAGGTTGCGATAATGTCCAATTTGCCGGAGCATCCGAACATAGTGAAGCTGAAGGCGACCTATGAGGACAACGAGAACGTTCACCTGGTGATGGAGCTCTGTGAAGGAGGCGAGCTTTTCGATAGGATTGTGGCGAGAGGGCACTACAGTGAACGAGCTGCGGCGAACGTAGCCAGGACTGTGGCTGAGGTGGTGAGGATGTGCCATGCGAATGGGGTTATGCACAGGGATTTGAAGCCGGAGAACTTTCTGTTTTCGAATAAGAAAGAGCACTCCCCGCTTAAGGCTATTGATTTTGGGCTCTCTGTGTTTTTTAAGCCTGGGGAGAAGTTTACGGAGATTGTGGGGAGTCCGTACTACATGGCACCCGAGGTTTTGAAGAGGAATTATGGACCCGAGGTTGATATATGGAGCGCCGGAGTGATTCTTTACATTTTGTTATGTGGGGTTCCTCCATTTTGGGCGGAGAGTGAACAGGGTGTGGCTCTTGCAATCTTGAGGGGGGTGATTGATTTCAAGAGGGAACCCTGGCCTCAGATTTCAGAGAGTGCTAAAAGCCTCGTAAGGCAGATGCTCGACCCGGATCCTAAAAAACGCTTGACTGCTCAACAGGTGCTTGAACATCCATGGTTACAAAATGCAAAGAAAGCTCCAAATGTTCCATTAGGAGATATTGTGAGGCCAAGGCTCAAGCAGTTCTCAATAATGAACAGATTTAAAAAGAAGGCCTTACGGGTAATTGCGGAGCACTTATCTGTTAAAGAAGTAGAAGTAATCAGGGACATGTTCACATTGATGGACACTGATAAAGATGGCAGAGTAACTTATGAGGAATTGAAGACTGGTCTTCAAAAGGTTGGTTCGCAGTTGGCTGAACCAGAGGTTAAGATGCTGATGGAAGTGGCTGATGTTGATGGGAATGGATTTCTGGATTATGGAGAATTCGCAGCAGTTACAATTCACTTGCAAAAATTGGAGAATGATGAACATCTCCGGAGAGCATTTATATTCTTTGACAAGGATGGAAGTGGGTATATTGAATTAGTTGAGCTAAGGCAAGCATTGGCAGATGAAACAGGTGAAACGGATATGGATGTGCTGACTGACATCATGCGTGAGGTGGATACTGACAAGGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009555 GO:0009628 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010152 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0021700 GO:0023052 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0048229 GO:0048518 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080134 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701 GO:1902584 GO:2000070

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 Pkinase

Protein Analysis

492

Amino Acids

55.72

Weight (kDa)

6.32

Isoelectric Point (pI)

39.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	67 - 324	5.8e-80	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	68 - 320	9.4e-28	Protein tyrosine and serine/threonine kinase
Kdo	PF06293	99 - 212	8.6e-06	Lipopolysaccharide kinase (Kdo/WaaP) family
EF-hand_7	PF13499	370 - 431	4e-11	EF-hand domain pair
EF-hand_1	PF00036	372 - 398	5e-07	EF hand domain
EF-hand_7	PF13499	442 - 491	6.3e-08	EF-hand domain pair
EF-hand_6	PF13405	444 - 469	4.5e-06	EF-hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0015782)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18890 AT1G74740
fragaria_vesca	FvH4_5g15720
malus_domestica	MD06G1186000.v1.1 MD14G1192200.v1.1
prunus_persica	Prupe.5G184800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0180391
rosa_laevigata	RLG00000005271
rosa_multiflora	Rmu_sc0003526.1_g000008
rosa_rugosa	Rorug06G0432800
rosa_samantha	Rh7AG033300 Rh7BG033200 Rh7CG035200 Rh7DG033700
rosa_wichuraiana	Rw7G002780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	36
AccB1I	GGYRCC	3 cut(s)	50, 133, 703
AccBSI	CCGCTC	2 cut(s)	91, 166
AccII	CGCG	4 cut(s)	119, 222, 258, 339
AccIII	TCCGGA	2 cut(s)	306, 1332
AclI	AACGTT	1 cut(s)	417
AclWI	GGATC	5 cut(s)	115, 133, 191, 917, 930
AcoI	YGGCCR	1 cut(s)	95
AcsI	RAATTY	1 cut(s)	1280
AcuI	CTGAAG	1 cut(s)	410
AcyI	GRCGYC	2 cut(s)	33, 134
AdeI	CACNNNGTG	1 cut(s)	427
AfaI	GTAC	3 cut(s)	197, 250, 695
AfiI	CCNNNNNNNGG	5 cut(s)	90, 130, 214, 430, 622
AflII	CTTAAG	1 cut(s)	620
AflIII	ACRYGT	2 cut(s)	15, 1122
AgsI	TTSAA	7 cut(s)	574, 718, 856, 959, 1177, 1190, 1379
AjiI	CACGTC	2 cut(s)	157, 331
AjnI	CCWGG	6 cut(s)	45, 57, 423, 512, 658, 866
AleI	CACNNNNGTG	1 cut(s)	486
AloI	GAACNNNNNNTCC	2 cut(s)	92, 124
Alw21I	GWGCWC	4 cut(s)	150, 438, 612, 1089
Alw26I	GTCTC	1 cut(s)	254
AlwI	GGATC	5 cut(s)	115, 133, 191, 917, 930
Ama87I	CYCGRG	2 cut(s)	707, 734
Aor13HI	TCCGGA	2 cut(s)	306, 1332
AoxI	GGCC	8 cut(s)	81, 95, 205, 217, 276, 869, 1019, 1065
ApeKI	GCWGC	2 cut(s)	497, 1286
ApoI	RAATTY	1 cut(s)	1280
Asp700I	GAANNNNTTC	1 cut(s)	104
AspLEI	GCGC	3 cut(s)	136, 339, 755
AspS9I	GGNCC	4 cut(s)	205, 217, 253, 731
AsuC2I	CCSGG	3 cut(s)	85, 209, 920
AsuHPI	GGTGA	7 cut(s)	164, 229, 413, 439, 544, 856, 1430
AsuII	TTCGAA	1 cut(s)	596
AvaI	CYCGRG	2 cut(s)	707, 734
AvaII	GGWCC	2 cut(s)	253, 731
BaeGI	GKGCMC	2 cut(s)	53, 483
BamHI	GGATCC	1 cut(s)	922
BanI	GGYRCC	3 cut(s)	50, 133, 703
BanII	GRGCYC	2 cut(s)	438, 642
BbsI	GAAGAC	2 cut(s)	1178, 1184
Bbv12I	GWGCWC	4 cut(s)	150, 438, 612, 1089
BbvCI	CCTCAGC	1 cut(s)	525
BbvI	GCAGC	2 cut(s)	484, 1298
BccI	CCATC	7 cut(s)	296, 424, 1129, 1145, 1228, 1247, 1355
BcgI	CGANNNNNNTGC	2 cut(s)	127, 161
BciT130I	CCWGG	6 cut(s)	47, 59, 425, 514, 660, 868
BciVI	GTATCC	1 cut(s)	1456
BcnI	CCSGG	3 cut(s)	85, 209, 920
BcoDI	GTCTC	1 cut(s)	254
BfmI	CTRYAG	1 cut(s)	484
BfoI	RGCGCY	2 cut(s)	137, 756
BfrI	CTTAAG	1 cut(s)	620
BfuI	GTATCC	1 cut(s)	1456
BisI	GCNGC	6 cut(s)	92, 95, 220, 498, 501, 1287
BlsI	GCNGC	6 cut(s)	93, 96, 221, 499, 502, 1288
Bme1390I	CCNGG	9 cut(s)	47, 59, 85, 209, 425, 514, 660, 868, 920
Bme18I	GGWCC	2 cut(s)	253, 731
BmeT110I	CYCGRG	2 cut(s)	707, 734
BmgBI	CACGTC	2 cut(s)	157, 331
BmgT120I	GGNCC	4 cut(s)	205, 217, 253, 731
BmiI	GGNNCC	7 cut(s)	52, 135, 705, 733, 789, 864, 924
BmrFI	CCNGG	9 cut(s)	47, 59, 85, 209, 425, 514, 660, 868, 920
BmrI	ACTGGG	1 cut(s)	212
BmsI	GCATC	4 cut(s)	243, 379, 900, 1218
BmuI	ACTGGG	1 cut(s)	212
BpiI	GAAGAC	2 cut(s)	1178, 1184
Bpu10I	CCTNAGC	2 cut(s)	525, 1391
Bpu14I	TTCGAA	1 cut(s)	596
BpuEI	CTTGAG	3 cut(s)	134, 856, 1013
BpuMI	CCSGG	3 cut(s)	85, 209, 920
BsaBI	GATNNNNATC	1 cut(s)	1326
BsaHI	GRCGYC	2 cut(s)	33, 134
BsaWI	WCCGGW	4 cut(s)	123, 174, 306, 1332
BsaXI	ACNNNNNCTCC	6 cut(s)	92, 122, 288, 318, 999, 1029
Bsc4I	CCNNNNNNNGG	5 cut(s)	90, 130, 214, 430, 622
Bse118I	RCCGGY	1 cut(s)	130
Bse1I	ACTGG	2 cut(s)	207, 1186
Bse8I	GATNNNNATC	1 cut(s)	1326
BseAI	TCCGGA	2 cut(s)	306, 1332
BseBI	CCWGG	6 cut(s)	47, 59, 425, 514, 660, 868
BseGI	GGATG	5 cut(s)	370, 543, 961, 1366, 1438
BseJI	GATNNNNATC	1 cut(s)	1326
BseLI	CCNNNNNNNGG	5 cut(s)	90, 130, 214, 430, 622
BseMII	CTCAG	2 cut(s)	516, 887
BseNI	ACTGG	2 cut(s)	207, 1186
BseSI	GKGCMC	2 cut(s)	53, 483
BseX3I	CGGCCG	1 cut(s)	95
BseXI	GCAGC	2 cut(s)	484, 1298
BseYI	CCCAGC	1 cut(s)	214
Bsh1236I	CGCG	4 cut(s)	119, 222, 258, 339
Bsh1285I	CGRYCG	2 cut(s)	98, 128
BshFI	GGCC	8 cut(s)	83, 97, 207, 219, 278, 871, 1021, 1067
BshNI	GGYRCC	3 cut(s)	50, 133, 703
BsiEI	CGRYCG	2 cut(s)	98, 128
BsiHKAI	GWGCWC	4 cut(s)	150, 438, 612, 1089
BsiHKCI	CYCGRG	2 cut(s)	707, 734
BslFI	GGGAC	1 cut(s)	1133
BslI	CCNNNNNNNGG	5 cut(s)	90, 130, 214, 430, 622
BsmAI	GTCTC	1 cut(s)	254
BsmFI	GGGAC	1 cut(s)	1133
BsnI	GGCC	8 cut(s)	83, 97, 207, 219, 278, 871, 1021, 1067
BsoBI	CYCGRG	2 cut(s)	707, 734
Bsp119I	TTCGAA	1 cut(s)	596
Bsp1286I	GDGCHC	7 cut(s)	53, 150, 438, 483, 612, 642, 1089
Bsp13I	TCCGGA	2 cut(s)	306, 1332
Bsp143I	GATC	4 cut(s)	120, 138, 183, 922
Bsp19I	CCATGG	1 cut(s)	965
BspANI	GGCC	8 cut(s)	83, 97, 207, 219, 278, 871, 1021, 1067
BspCNI	CTCAG	2 cut(s)	517, 886
BspEI	TCCGGA	2 cut(s)	306, 1332
BspFNI	CGCG	4 cut(s)	119, 222, 258, 339
BspLI	GGNNCC	7 cut(s)	52, 135, 705, 733, 789, 864, 924
BspPI	GGATC	5 cut(s)	115, 133, 191, 917, 930
BspT104I	TTCGAA	1 cut(s)	596
BspT107I	GGYRCC	3 cut(s)	50, 133, 703
BspTI	CTTAAG	1 cut(s)	620
BsrBI	CCGCTC	2 cut(s)	91, 166
BsrFI	RCCGGY	1 cut(s)	130
BsrI	ACTGG	2 cut(s)	207, 1186
BssAI	RCCGGY	1 cut(s)	130
BssMI	GATC	4 cut(s)	120, 138, 183, 922
BssNI	GRCGYC	2 cut(s)	33, 134
BssT1I	CCWWGG	2 cut(s)	965, 1022
Bst2UI	CCWGG	6 cut(s)	47, 59, 425, 514, 660, 868
Bst4CI	ACNGT	2 cut(s)	488, 520
Bst6I	CTCTTC	1 cut(s)	713
BstACI	GRCGYC	2 cut(s)	33, 134
BstAFI	CTTAAG	1 cut(s)	620
BstAPI	GCANNNNNTGC	1 cut(s)	18
BstBI	TTCGAA	1 cut(s)	596
BstC8I	GCNNGC	4 cut(s)	132, 280, 452, 1398
BstDEI	CTNAG	3 cut(s)	525, 873, 1391
BstDSI	CCRYGG	2 cut(s)	220, 965
BstEII	GGTNACC	1 cut(s)	170
BstF5I	GGATG	5 cut(s)	370, 543, 961, 1366, 1438
BstFNI	CGCG	4 cut(s)	119, 222, 258, 339
BstH2I	RGCGCY	2 cut(s)	137, 756
BstHHI	GCGC	3 cut(s)	136, 339, 755
BstKTI	GATC	4 cut(s)	123, 141, 186, 925
BstMAI	GTCTC	1 cut(s)	254
BstMBI	GATC	4 cut(s)	120, 138, 183, 922
BstMCI	CGRYCG	2 cut(s)	98, 128
BstMWI	GCNNNNNNNGC	4 cut(s)	18, 509, 616, 986
BstNI	CCWGG	6 cut(s)	47, 59, 425, 514, 660, 868
BstNSI	RCATGY	1 cut(s)	1126
BstPI	GGTNACC	1 cut(s)	170
BstSCI	CCNGG	9 cut(s)	45, 57, 83, 207, 423, 512, 658, 866, 918
BstSFI	CTRYAG	1 cut(s)	484
BstSLI	GKGCMC	2 cut(s)	53, 483
BstUI	CGCG	4 cut(s)	119, 222, 258, 339
BstV1I	GCAGC	2 cut(s)	484, 1298
BstV2I	GAAGAC	2 cut(s)	1178, 1184
BstX2I	RGATCY	1 cut(s)	922
BstXI	CCANNNNNNTGG	2 cut(s)	520, 551
BstYI	RGATCY	1 cut(s)	922
BstZI	CGGCCG	1 cut(s)	95
BsuI	GTATCC	1 cut(s)	1456
BsuRI	GGCC	8 cut(s)	83, 97, 207, 219, 278, 871, 1021, 1067
BtgI	CCRYGG	2 cut(s)	220, 965
BtrI	CACGTC	2 cut(s)	157, 331
BtsCI	GGATG	5 cut(s)	370, 543, 961, 1366, 1438
BtsIMutI	CAGTG	2 cut(s)	493, 1140
Cac8I	GCNNGC	4 cut(s)	132, 280, 452, 1398
CfoI	GCGC	3 cut(s)	136, 339, 755
Cfr10I	RCCGGY	1 cut(s)	130
Cfr13I	GGNCC	4 cut(s)	205, 217, 253, 731
Cfr42I	CCGCGG	1 cut(s)	223
CpoI	CGGWCCG	1 cut(s)	253
CseI	GACGC	2 cut(s)	10, 125
CsiI	ACCWGGT	1 cut(s)	423
Csp6I	GTAC	3 cut(s)	196, 249, 694
CspI	CGGWCCG	1 cut(s)	253
CviAII	CATG	5 cut(s)	545, 700, 966, 1123, 1450
CviQI	GTAC	3 cut(s)	196, 249, 694
DdeI	CTNAG	3 cut(s)	525, 873, 1391
DinI	GGCGCC	1 cut(s)	135
DpnI	GATC	4 cut(s)	122, 140, 185, 924
DpnII	GATC	4 cut(s)	120, 138, 183, 922
DraI	TTTAAA	1 cut(s)	1057
DraIII	CACNNNGTG	1 cut(s)	427
EaeI	YGGCCR	1 cut(s)	95
EagI	CGGCCG	1 cut(s)	95
Eam1104I	CTCTTC	1 cut(s)	713
EarI	CTCTTC	1 cut(s)	713
EciI	GGCGGA	1 cut(s)	818
Ecl136II	GAGCTC	1 cut(s)	436
EclXI	CGGCCG	1 cut(s)	95
Eco130I	CCWWGG	2 cut(s)	965, 1022
Eco147I	AGGCCT	1 cut(s)	1067
Eco24I	GRGCYC	2 cut(s)	438, 642
Eco47I	GGWCC	2 cut(s)	253, 731
Eco52I	CGGCCG	1 cut(s)	95
Eco53kI	GAGCTC	1 cut(s)	436
Eco57I	CTGAAG	1 cut(s)	410
Eco88I	CYCGRG	2 cut(s)	707, 734
Eco91I	GGTNACC	1 cut(s)	170
EcoICRI	GAGCTC	1 cut(s)	436
EcoO65I	GGTNACC	1 cut(s)	170
EcoRI	GAATTC	1 cut(s)	1280
EcoRII	CCWGG	6 cut(s)	45, 57, 423, 512, 658, 866
EcoT14I	CCWWGG	2 cut(s)	965, 1022
EcoT38I	GRGCYC	2 cut(s)	438, 642
EgeI	GGCGCC	1 cut(s)	135
EheI	GGCGCC	1 cut(s)	135
ErhI	CCWWGG	2 cut(s)	965, 1022
FaeI	CATG	5 cut(s)	548, 703, 969, 1126, 1453
FalI	AAGNNNNNCTT	2 cut(s)	1052, 1084
FaqI	GGGAC	1 cut(s)	1133
FatI	CATG	5 cut(s)	544, 699, 965, 1122, 1449
FauI	CCCGC	2 cut(s)	215, 624
Fnu4HI	GCNGC	6 cut(s)	92, 95, 220, 498, 501, 1287
FokI	GGATG	5 cut(s)	357, 550, 948, 1373, 1445
FriOI	GRGCYC	2 cut(s)	438, 642
Fsp4HI	GCNGC	6 cut(s)	92, 95, 220, 498, 501, 1287
GlaI	GCGC	3 cut(s)	135, 338, 754
GluI	GCNGC	6 cut(s)	92, 95, 220, 498, 501, 1287
GsaI	CCCAGC	1 cut(s)	218
HaeII	RGCGCY	2 cut(s)	137, 756
HaeIII	GGCC	8 cut(s)	83, 97, 207, 219, 278, 871, 1021, 1067
HgaI	GACGC	2 cut(s)	10, 125
HhaI	GCGC	3 cut(s)	136, 339, 755
Hin1I	GRCGYC	2 cut(s)	33, 134
Hin1II	CATG	5 cut(s)	548, 703, 969, 1126, 1453
Hin6I	GCGC	3 cut(s)	134, 337, 753
HinP1I	GCGC	3 cut(s)	134, 337, 753
HinfI	GANTC	4 cut(s)	160, 178, 688, 763
HphI	GGTGA	7 cut(s)	164, 229, 413, 439, 544, 856, 1430
Hpy166II	GTNNAC	5 cut(s)	421, 491, 672, 812, 1128
Hpy188I	TCNGA	7 cut(s)	26, 112, 143, 183, 375, 876, 883
Hpy188III	TCNNGA	6 cut(s)	40, 307, 835, 856, 1268, 1333
Hpy8I	GTNNAC	5 cut(s)	421, 491, 672, 812, 1128
HpyAV	CCTTC	4 cut(s)	73, 385, 437, 1057
HpyCH4III	ACNGT	2 cut(s)	488, 520
HpyCH4IV	ACGT	6 cut(s)	15, 33, 156, 330, 417, 507
HpyCH4V	TGCA	6 cut(s)	12, 282, 562, 830, 980, 1306
HpyF10VI	GCNNNNNNNGC	4 cut(s)	18, 509, 616, 986
HpyF3I	CTNAG	3 cut(s)	525, 873, 1391
HpySE526I	ACGT	6 cut(s)	15, 33, 156, 330, 417, 507
Hsp92I	GRCGYC	2 cut(s)	33, 134
Hsp92II	CATG	5 cut(s)	548, 703, 969, 1126, 1453
HspAI	GCGC	3 cut(s)	134, 337, 753
KasI	GGCGCC	1 cut(s)	133
Kpn2I	TCCGGA	2 cut(s)	306, 1332
KroI	GCCGGC	1 cut(s)	130
KroNI	GCCGGC	1 cut(s)	132
KspI	CCGCGG	1 cut(s)	223
Kzo9I	GATC	4 cut(s)	120, 138, 183, 922
LmnI	GCTCC	8 cut(s)	88, 211, 309, 367, 433, 750, 994, 1084
Lsp1109I	GCAGC	2 cut(s)	484, 1298
LweI	GCATC	4 cut(s)	243, 379, 900, 1218
MabI	ACCWGGT	1 cut(s)	423
MaeII	ACGT	6 cut(s)	15, 33, 156, 330, 417, 507
MaeIII	GTNAC	4 cut(s)	170, 969, 1159, 1291
MalI	GATC	4 cut(s)	122, 140, 185, 924
MbiI	CCGCTC	2 cut(s)	91, 166
MboI	GATC	4 cut(s)	120, 138, 183, 922
MboII	GAAGA	5 cut(s)	85, 88, 730, 1178, 1189
MflI	RGATCY	1 cut(s)	922
MhlI	GDGCHC	7 cut(s)	53, 150, 438, 483, 612, 642, 1089
MluCI	AATT	8 cut(s)	358, 724, 1077, 1172, 1280, 1296, 1310, 1379
Mly113I	GGCGCC	1 cut(s)	134
MlyI	GAGTC	2 cut(s)	172, 697
MreI	CGCCGGCG	1 cut(s)	130
MroI	TCCGGA	2 cut(s)	306, 1332
MroNI	GCCGGC	1 cut(s)	130
MroXI	GAANNNNTTC	1 cut(s)	104
MseI	TTAA	5 cut(s)	621, 654, 1056, 1098, 1224
MslI	CAYNNNNRTG	3 cut(s)	486, 549, 1133
MspA1I	CMGCKG	1 cut(s)	222
MspCI	CTTAAG	1 cut(s)	620
MspR9I	CCNGG	9 cut(s)	47, 59, 85, 209, 425, 514, 660, 868, 920
MvaI	CCWGG	6 cut(s)	47, 59, 425, 514, 660, 868
MvnI	CGCG	4 cut(s)	119, 222, 258, 339
MwoI	GCNNNNNNNGC	4 cut(s)	18, 509, 616, 986
NaeI	GCCGGC	1 cut(s)	132
NarI	GGCGCC	1 cut(s)	134
NciI	CCSGG	3 cut(s)	85, 209, 920
NcoI	CCATGG	1 cut(s)	965
NdeII	GATC	4 cut(s)	120, 138, 183, 922
NgoMIV	GCCGGC	1 cut(s)	130
NlaIII	CATG	5 cut(s)	548, 703, 969, 1126, 1453
NlaIV	GGNNCC	7 cut(s)	52, 135, 705, 733, 789, 864, 924
NmuCI	GTSAC	1 cut(s)	170
NspI	RCATGY	1 cut(s)	1126
NspV	TTCGAA	1 cut(s)	596
OliI	CACNNNNGTG	1 cut(s)	486
PceI	AGGCCT	1 cut(s)	1067
PciI	ACATGT	1 cut(s)	1122
PcsI	WCGNNNNNNNCGW	2 cut(s)	134, 336
PdiI	GCCGGC	1 cut(s)	132
PdmI	GAANNNNTTC	1 cut(s)	104
PfeI	GAWTC	2 cut(s)	160, 763
PkrI	GCNGC	6 cut(s)	93, 96, 221, 499, 502, 1288
PleI	GAGTC	2 cut(s)	172, 696
PluTI	GGCGCC	1 cut(s)	137
PpsI	GAGTC	2 cut(s)	172, 696
PscI	ACATGT	1 cut(s)	1122
Psp124BI	GAGCTC	1 cut(s)	438
Psp1406I	AACGTT	1 cut(s)	417
Psp6I	CCWGG	6 cut(s)	45, 57, 423, 512, 658, 866
PspEI	GGTNACC	1 cut(s)	170
PspFI	CCCAGC	1 cut(s)	214
PspGI	CCWGG	6 cut(s)	45, 57, 423, 512, 658, 866
PspN4I	GGNNCC	7 cut(s)	52, 135, 705, 733, 789, 864, 924
PspPI	GGNCC	4 cut(s)	205, 217, 253, 731
PsuI	RGATCY	1 cut(s)	922
RsaI	GTAC	3 cut(s)	197, 250, 695
RsaNI	GTAC	3 cut(s)	196, 249, 694
RseI	CAYNNNNRTG	3 cut(s)	486, 549, 1133
Rsr2I	CGGWCCG	1 cut(s)	253
RsrII	CGGWCCG	1 cut(s)	253
SacI	GAGCTC	1 cut(s)	438
SacII	CCGCGG	1 cut(s)	223
SaqAI	TTAA	5 cut(s)	621, 654, 1056, 1098, 1224
SatI	GCNGC	6 cut(s)	92, 95, 220, 498, 501, 1287
Sau3AI	GATC	4 cut(s)	120, 138, 183, 922
Sau96I	GGNCC	4 cut(s)	205, 217, 253, 731
SchI	GAGTC	2 cut(s)	172, 697
ScrFI	CCNGG	9 cut(s)	47, 59, 85, 209, 425, 514, 660, 868, 920
SduI	GDGCHC	7 cut(s)	53, 150, 438, 483, 612, 642, 1089
SexAI	ACCWGGT	1 cut(s)	423
SfaNI	GCATC	4 cut(s)	243, 379, 900, 1218
SfcI	CTRYAG	1 cut(s)	484
SfoI	GGCGCC	1 cut(s)	135
Sfr303I	CCGCGG	1 cut(s)	223
SfuI	TTCGAA	1 cut(s)	596
SgrAI	CRCCGGYG	1 cut(s)	130
SgrBI	CCGCGG	1 cut(s)	223
SinI	GGWCC	2 cut(s)	253, 731
SmiMI	CAYNNNNRTG	3 cut(s)	486, 549, 1133
SmlI	CTYRAG	4 cut(s)	149, 620, 835, 1028
SmoI	CTYRAG	4 cut(s)	149, 620, 835, 1028
Sse9I	AATT	8 cut(s)	358, 724, 1077, 1172, 1280, 1296, 1310, 1379
SseBI	AGGCCT	1 cut(s)	1067
SspDI	GGCGCC	1 cut(s)	133
SstI	GAGCTC	1 cut(s)	438
StuI	AGGCCT	1 cut(s)	1067
StyD4I	CCNGG	9 cut(s)	45, 57, 83, 207, 423, 512, 658, 866, 918
StyI	CCWWGG	2 cut(s)	965, 1022
TaaI	ACNGT	2 cut(s)	488, 520
TaiI	ACGT	6 cut(s)	18, 36, 159, 333, 420, 510
TaqI	TCGA	4 cut(s)	39, 459, 596, 915
TaqII	GACCGA	1 cut(s)	158
TasI	AATT	8 cut(s)	358, 724, 1077, 1172, 1280, 1296, 1310, 1379
TatI	WGTACW	1 cut(s)	195
TauI	GCSGC	4 cut(s)	94, 97, 222, 503
TfiI	GAWTC	2 cut(s)	160, 763
Tru1I	TTAA	5 cut(s)	621, 654, 1056, 1098, 1224
Tru9I	TTAA	5 cut(s)	621, 654, 1056, 1098, 1224
TscAI	CASTG	2 cut(s)	493, 1147
TseFI	GTSAC	1 cut(s)	170
TseI	GCWGC	2 cut(s)	497, 1286
Tsp45I	GTSAC	1 cut(s)	170
TspDTI	ATGAA	3 cut(s)	1061, 1338, 1425
TspGWI	ACGGA	5 cut(s)	266, 313, 681, 689, 1439
TspRI	CASTG	2 cut(s)	493, 1147
Vha464I	CTTAAG	1 cut(s)	620
VpaK11BI	GGWCC	2 cut(s)	253, 731
XapI	RAATTY	1 cut(s)	1280
XceI	RCATGY	1 cut(s)	1126
XmnI	GAANNNNTTC	1 cut(s)	104
ZraI	GACGTC	1 cut(s)	34

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.