Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0016338

Physical Location & Seq

Genomic Coordinates Reverse (-)

34111471 .. 34114727

Gene Structure 3257 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1206300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2061 bp

ATGGCGGGTGACACAGACAGCCCGTCTTCTCCCCAAAACAAACGGCGGAAAACCCTAAAATCAAAAATCGAAGAGGACCCAATTCCCCACGAACCCCAAGAACCTGAAAATTCATCTTCTCAGCGATGCCCAATTTGCTTCCTGGACGGTGGCAAAGTGATCAGAGGCGAAATCGATTGCTGCGACCACTACTTCTGCTTTCTCTGCATTATGGAGTGGTCGAAGAAGGAGTCGCACTGCCCACTCTGCCGCCGCAGATTCACCACCATTCGCCGGCCGCCGAAAGACGGCGTCTTTGCCCGCGAGCGAGTCGTCAAGATTCCTGCTTGTGATCATGCTCACCATCCATCTAGCAACATGGCCGGGCAAGTTCTTCGTCACGAAGAAGTTTCGTGTCAAGTGTGCCACGGCATGGCAGATGAATATCTTCTGTTGCTCTGCGATATTTGCGATTCAGCTGCTCATACGTACTGTGTTGGACTGGGTAATACTGTGCCCGAAGGTGATTGGTTCTGCCTTGACTGCACTACTATTAGGGGAGAACACGATGACAACGTTGATTCTAGTTCTGATTCTGACGATGAATATCTTTTAGGACCATCAGACCCAAATATATCGGCCTTTGACATTGTACAAGAATCATATGGTCATAGCTCGGCGGCTAATAGGTATCTCCCAAGAATCCCTAGAATTCCAGACCACTTTGCACTTCCAGTTGTCCGTGACAGGTCTACCAGTATCGCGGATGAAGTAATTACCACGCCAGCAGAAACACTACCAAGTGTTGCAGCATCAGGTGCAAGAACATTGGTTCACCGTCGCTATGTGTGCACTCGAGTACAAGAACTCCGCGAAAATTGGAATGCCATCCAAAGTGGTTCATTGAGCTTTACTTCGAGTTCAATTAAATCTGGCTGCATTAGTAGTCAAAAGCCTAAAAATGGTGTGATAATCCATGAAAGATCAGGCCAAACACAGTCCTCATCTTCCACAAGTGGTCAACAACCGAGAAATCGAGACAGTTGTGGCTTAAGTGATATCGACAAAGCATGGAAAATGATGGAAATTGCAAAGTCAAGAATGGATGCTGGTAAAAAGTGCAGCGGCAGTTCCGTCAGCACAACAAGTGATTCAAGAAAACGAACTAATGTAAATCCTTGTTTTGGTACTTCAAGTCAAGCACAATCCTCATCTTCCGCAGGGTGTCATCGACCAATAAATCAAGTTGGTTGTGGAACACGTGACATTAAGTCGAAACAGCACGTTCATACAAACACCGGCAGCAGCAGTGGCGTTCAGCACCCAAAAGTTCTTTCTGGGATATCAAGTGCTTCAAAAGAAGCAACAAATGTAACATCGAGTTCTGATATTTTGAGAAGCCAACAACTTGGAGCCTGCGATGGGAGAAAAACCGGAAAGGAGTCCTACAAATGCAGTCCTCGTGAAAAAGAAACAATAAGACATCAACCTGTAAAGTTGGAAAAGGATACGCAGTTTAGGGTTACGCCTCAGGATGCAGTCGCGCCTAGCGAAGGCCCTTCAGCTAGACATATGAAGCAGGCTGCAGCAAACATCATTCATGAGCAAAATAGGTCTGCTTGTTCAGTAAATGAAGGAGCACCTTCTTCTTTTTATGCCAAACCAGAGATCAGTACTTCTTCCAGTAAATTAGATGCGCAGAAGAGAAATATTGGCCGGGGAGAGAGCTGTGTGGAAGGCCAATCAAGAAAAGACGCCAAAAGTGAAATCGCGTCTGTTGTGAAGGTGAACTTGAAGATGTTAACCAAAGACAAACCATTAGGAGTTGATGCATTCAAGGAAATTGCAAGGCTTTCCACGCATACCATTTATGGCGCATGTGGTTTAAAGCGTTCAAAGTCTGGTATGTACTCCTTCCCTAGCTTAGAATGCCCCCACACCGAGCAGTTTCAGCCGCCGCACAAACCCAGTCCGATGCCAAACTCTTGTCGAGCTTGCTTTGATGGTTTTGTGAAAGGCGTAGTCAGCTCTATTCTGTGTGGTTCAGCTGGTTCCACAAAATTAAGCCGAGCTCCATCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0006139 GO:0006351 GO:0006366 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0018130 GO:0019438 GO:0019899 GO:0019904 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0046483 GO:0070063 GO:0071704 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PHD zf-RING_2

Protein Analysis

687

Amino Acids

74.69

Weight (kDa)

8.64

Isoelectric Point (pI)

62.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	42 - 84	1.2e-06	Ring finger domain
PHD	PF00628	132 - 175	2e-07	PHD-finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016338)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g13310
malus_domestica	MD06G1206300.v1.1
prunus_persica	Prupe.5G210700_v2.0.a1 Prupe.5G210700_v2.0.a1
pyrus_communis	pycom06g18420
rosa_chinensis	RchiOBHm_Chr7g0183831
rosa_laevigata	RLG00000005017
rosa_multiflora	Rmu_sc0013966.1_g000004
rosa_roxburghii	Rroxscaffold_3G00270150
rosa_rugosa	Rorug06G0461100
rosa_samantha	Rh7AG061800 Rh7BG061900 Rh7CG063400 Rh7DG061600
rosa_wichuraiana	Rw7G005110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1677
AccB7I	CCANNNNNTGG	1 cut(s)	412
AccI	GTMKAC	1 cut(s)	731
AccII	CGCG	5 cut(s)	303, 743, 852, 1523, 1751
AclI	AACGTT	1 cut(s)	555
AcoI	YGGCCR	3 cut(s)	275, 360, 1693
AcsI	RAATTY	2 cut(s)	109, 690
AcuI	CTGAAG	1 cut(s)	1524
AcvI	CACGTG	1 cut(s)	1241
AcyI	GRCGYC	2 cut(s)	291, 1734
AfaI	GTAC	6 cut(s)	470, 633, 840, 1168, 1654, 1889
AfiI	CCNNNNNNNGG	7 cut(s)	273, 287, 412, 941, 1163, 1401, 1532
AflII	CTTAAG	1 cut(s)	1030
AflIII	ACRYGT	1 cut(s)	1238
AgsI	TTSAA	7 cut(s)	903, 1134, 1173, 1335, 1774, 1816, 1875
AhdI	GACNNNNNGTC	2 cut(s)	22, 1249
AjnI	CCWGG	1 cut(s)	141
AjuI	GAANNNNNNNTTGG	2 cut(s)	1297, 1329
Alw21I	GWGCWC	3 cut(s)	833, 1621, 2053
Alw26I	GTCTC	1 cut(s)	1011
Alw44I	GTGCAC	1 cut(s)	829
Ama87I	CYCGRG	1 cut(s)	834
AoxI	GGCC	7 cut(s)	275, 360, 618, 967, 1534, 1693, 1717
ApaLI	GTGCAC	1 cut(s)	829
ApeKI	GCWGC	9 cut(s)	180, 458, 788, 915, 1101, 1281, 1284, 1562, 1565
ApoI	RAATTY	2 cut(s)	109, 690
ArsI	GACNNNNNNTTYG	4 cut(s)	215, 247, 278, 310
Asp700I	GAANNNNTTC	1 cut(s)	426
AspLEI	GCGC	3 cut(s)	1525, 1678, 1856
AspS9I	GGNCC	3 cut(s)	76, 596, 1535
AsuC2I	CCSGG	2 cut(s)	364, 1697
AsuHPI	GGTGA	6 cut(s)	20, 253, 332, 515, 806, 1777
AvaI	CYCGRG	1 cut(s)	834
AvaII	GGWCC	2 cut(s)	76, 596
AxyI	CCTNAGG	1 cut(s)	1509
BaeGI	GKGCMC	2 cut(s)	498, 833
BanII	GRGCYC	1 cut(s)	2053
BauI	CACGAG	1 cut(s)	1440
BbrPI	CACGTG	1 cut(s)	1241
BbsI	GAAGAC	1 cut(s)	18
Bbv12I	GWGCWC	3 cut(s)	833, 1621, 2053
BbvI	GCAGC	9 cut(s)	167, 445, 800, 902, 1113, 1293, 1296, 1549, 1577
BccI	CCATC	7 cut(s)	351, 355, 607, 875, 1054, 1394, 1976
BceAI	ACGGC	3 cut(s)	59, 304, 424
BcgI	CGANNNNNNTGC	4 cut(s)	356, 390, 440, 474
BciT130I	CCWGG	1 cut(s)	143
BciVI	GTATCC	1 cut(s)	1480
BclI	TGATCA	2 cut(s)	159, 331
BcnI	CCSGG	2 cut(s)	364, 1697
BcoDI	GTCTC	1 cut(s)	1011
BfaI	CTAG	6 cut(s)	351, 564, 687, 1527, 1545, 1899
BfmI	CTRYAG	1 cut(s)	1563
BfrI	CTTAAG	1 cut(s)	1030
BfuI	GTATCC	1 cut(s)	1480
BmcAI	AGTACT	1 cut(s)	1654
Bme1390I	CCNGG	3 cut(s)	143, 364, 1697
Bme18I	GGWCC	2 cut(s)	76, 596
BmeRI	GACNNNNNGTC	2 cut(s)	22, 1249
BmeT110I	CYCGRG	1 cut(s)	834
BmgT120I	GGNCC	3 cut(s)	76, 596, 1535
BmiI	GGNNCC	3 cut(s)	78, 1393, 2032
BmrFI	CCNGG	3 cut(s)	143, 364, 1697
BmrI	ACTGGG	2 cut(s)	491, 1941
BmsI	GCATC	7 cut(s)	116, 800, 1075, 1504, 1663, 1798, 1944
BmuI	ACTGGG	2 cut(s)	491, 1941
BpiI	GAAGAC	1 cut(s)	18
BpuMI	CCSGG	2 cut(s)	364, 1697
Bsa29I	ATCGAT	1 cut(s)	174
BsaAI	YACGTR	2 cut(s)	468, 1241
BsaBI	GATNNNNATC	2 cut(s)	423, 585
BsaHI	GRCGYC	2 cut(s)	291, 1734
BsaJI	CCNNGG	2 cut(s)	406, 1696
BsaWI	WCCGGW	1 cut(s)	1412
BsaXI	ACNNNNNCTCC	2 cut(s)	831, 861
Bsc4I	CCNNNNNNNGG	7 cut(s)	273, 287, 412, 941, 1163, 1401, 1532
Bse118I	RCCGGY	2 cut(s)	273, 1277
Bse1I	ACTGG	5 cut(s)	486, 713, 735, 1662, 1947
Bse21I	CCTNAGG	1 cut(s)	1509
Bse8I	GATNNNNATC	2 cut(s)	423, 585
BseBI	CCWGG	1 cut(s)	143
BseCI	ATCGAT	1 cut(s)	174
BseDI	CCNNGG	2 cut(s)	406, 1696
BseGI	GGATG	5 cut(s)	343, 751, 867, 1090, 1519
BseJI	GATNNNNATC	2 cut(s)	423, 585
BseLI	CCNNNNNNNGG	7 cut(s)	273, 287, 412, 941, 1163, 1401, 1532
BseMII	CTCAG	2 cut(s)	134, 1523
BseNI	ACTGG	5 cut(s)	486, 713, 735, 1662, 1947
BseSI	GKGCMC	2 cut(s)	498, 833
BseX3I	CGGCCG	1 cut(s)	275
BseXI	GCAGC	9 cut(s)	167, 445, 800, 902, 1113, 1293, 1296, 1549, 1577
BsgI	GTGCAG	2 cut(s)	508, 1120
Bsh1236I	CGCG	5 cut(s)	303, 743, 852, 1523, 1751
Bsh1285I	CGRYCG	1 cut(s)	278
BshFI	GGCC	7 cut(s)	277, 362, 620, 969, 1536, 1695, 1719
BshVI	ATCGAT	1 cut(s)	174
BsiEI	CGRYCG	1 cut(s)	278
BsiHKAI	GWGCWC	3 cut(s)	833, 1621, 2053
BsiHKCI	CYCGRG	1 cut(s)	834
BsiSI	CCGG	5 cut(s)	274, 363, 1278, 1413, 1696
BslI	CCNNNNNNNGG	7 cut(s)	273, 287, 412, 941, 1163, 1401, 1532
BsmAI	GTCTC	1 cut(s)	1011
BsmI	GAATGC	3 cut(s)	868, 1811, 1913
BsnI	GGCC	7 cut(s)	277, 362, 620, 969, 1536, 1695, 1719
BsoBI	CYCGRG	1 cut(s)	834
Bsp1286I	GDGCHC	4 cut(s)	498, 833, 1621, 2053
Bsp1407I	TGTACA	1 cut(s)	631
Bsp143I	GATC	4 cut(s)	159, 331, 962, 1647
BspANI	GGCC	7 cut(s)	277, 362, 620, 969, 1536, 1695, 1719
BspCNI	CTCAG	2 cut(s)	133, 1522
BspDI	ATCGAT	1 cut(s)	174
BspFNI	CGCG	5 cut(s)	303, 743, 852, 1523, 1751
BspHI	TCATGA	1 cut(s)	1579
BspLI	GGNNCC	3 cut(s)	78, 1393, 2032
BspMAI	CTGCAG	1 cut(s)	1567
BspTI	CTTAAG	1 cut(s)	1030
BsrFI	RCCGGY	2 cut(s)	273, 1277
BsrGI	TGTACA	1 cut(s)	631
BsrI	ACTGG	5 cut(s)	486, 713, 735, 1662, 1947
BssAI	RCCGGY	2 cut(s)	273, 1277
BssECI	CCNNGG	2 cut(s)	406, 1696
BssMI	GATC	4 cut(s)	159, 331, 962, 1647
BssNI	GRCGYC	2 cut(s)	291, 1734
BssSI	CACGAG	1 cut(s)	1440
Bst2BI	CACGAG	1 cut(s)	1440
Bst2UI	CCWGG	1 cut(s)	143
Bst4CI	ACNGT	6 cut(s)	149, 473, 493, 818, 978, 1022
Bst6I	CTCTTC	2 cut(s)	66, 1676
BstACI	GRCGYC	2 cut(s)	291, 1734
BstAFI	CTTAAG	1 cut(s)	1030
BstAPI	GCANNNNNTGC	1 cut(s)	797
BstAUI	TGTACA	1 cut(s)	631
BstBAI	YACGTR	2 cut(s)	468, 1241
BstC8I	GCNNGC	7 cut(s)	275, 301, 305, 765, 1396, 1560, 1975
BstDEI	CTNAG	3 cut(s)	120, 1509, 1903
BstDSI	CCRYGG	1 cut(s)	406
BstENI	CCTNNNNNAGG	1 cut(s)	1530
BstF5I	GGATG	5 cut(s)	343, 751, 867, 1090, 1519
BstFNI	CGCG	5 cut(s)	303, 743, 852, 1523, 1751
BstHHI	GCGC	3 cut(s)	1525, 1678, 1856
BstKTI	GATC	4 cut(s)	162, 334, 965, 1650
BstMAI	GTCTC	1 cut(s)	1011
BstMBI	GATC	4 cut(s)	159, 331, 962, 1647
BstMCI	CGRYCG	1 cut(s)	278
BstNI	CCWGG	1 cut(s)	143
BstNSI	RCATGY	1 cut(s)	1860
BstSCI	CCNGG	3 cut(s)	141, 362, 1695
BstSFI	CTRYAG	1 cut(s)	1563
BstSLI	GKGCMC	2 cut(s)	498, 833
BstSNI	TACGTA	1 cut(s)	468
BstUI	CGCG	5 cut(s)	303, 743, 852, 1523, 1751
BstV1I	GCAGC	9 cut(s)	167, 445, 800, 902, 1113, 1293, 1296, 1549, 1577
BstV2I	GAAGAC	1 cut(s)	18
BstXI	CCANNNNNNTGG	1 cut(s)	1388
BstZI	CGGCCG	1 cut(s)	275
Bsu15I	ATCGAT	1 cut(s)	174
Bsu36I	CCTNAGG	1 cut(s)	1509
BsuI	GTATCC	1 cut(s)	1480
BsuRI	GGCC	7 cut(s)	277, 362, 620, 969, 1536, 1695, 1719
BsuTUI	ATCGAT	1 cut(s)	174
BtgI	CCRYGG	1 cut(s)	406
BtgZI	GCGATG	2 cut(s)	139, 1413
BtsCI	GGATG	5 cut(s)	343, 751, 867, 1090, 1519
BtsI	GCAGTG	2 cut(s)	235, 1294
BtsIMutI	CAGTG	2 cut(s)	235, 1294
Cac8I	GCNNGC	7 cut(s)	275, 301, 305, 765, 1396, 1560, 1975
CciI	TCATGA	1 cut(s)	1579
CfoI	GCGC	3 cut(s)	1525, 1678, 1856
Cfr10I	RCCGGY	2 cut(s)	273, 1277
Cfr13I	GGNCC	3 cut(s)	76, 596, 1535
ClaI	ATCGAT	1 cut(s)	174
CseI	GACGC	3 cut(s)	280, 1740, 1742
Csp6I	GTAC	6 cut(s)	469, 632, 839, 1167, 1653, 1888
CviAII	CATG	7 cut(s)	335, 358, 412, 956, 1050, 1580, 1857
CviQI	GTAC	6 cut(s)	469, 632, 839, 1167, 1653, 1888
DdeI	CTNAG	3 cut(s)	120, 1509, 1903
DpnI	GATC	4 cut(s)	161, 333, 964, 1649
DpnII	GATC	4 cut(s)	159, 331, 962, 1647
DraI	TTTAAA	1 cut(s)	1866
DriI	GACNNNNNGTC	2 cut(s)	22, 1249
EaeI	YGGCCR	3 cut(s)	275, 360, 1693
EagI	CGGCCG	1 cut(s)	275
Eam1104I	CTCTTC	2 cut(s)	66, 1676
Eam1105I	GACNNNNNGTC	2 cut(s)	22, 1249
EarI	CTCTTC	2 cut(s)	66, 1676
EciI	GGCGGA	1 cut(s)	61
Ecl136II	GAGCTC	1 cut(s)	2051
EclXI	CGGCCG	1 cut(s)	275
Eco105I	TACGTA	1 cut(s)	468
Eco24I	GRGCYC	1 cut(s)	2053
Eco32I	GATATC	2 cut(s)	1039, 1323
Eco47I	GGWCC	2 cut(s)	76, 596
Eco52I	CGGCCG	1 cut(s)	275
Eco53kI	GAGCTC	1 cut(s)	2051
Eco57I	CTGAAG	1 cut(s)	1524
Eco72I	CACGTG	1 cut(s)	1241
Eco81I	CCTNAGG	1 cut(s)	1509
Eco88I	CYCGRG	1 cut(s)	834
EcoICRI	GAGCTC	1 cut(s)	2051
EcoNI	CCTNNNNNAGG	1 cut(s)	1530
EcoO109I	RGGNCCY	2 cut(s)	76, 1535
EcoRI	GAATTC	1 cut(s)	690
EcoRII	CCWGG	1 cut(s)	141
EcoRV	GATATC	2 cut(s)	1039, 1323
EcoT22I	ATGCAT	1 cut(s)	1813
EcoT38I	GRGCYC	1 cut(s)	2053
FaeI	CATG	7 cut(s)	338, 361, 415, 959, 1053, 1583, 1860
FalI	AAGNNNNNCTT	2 cut(s)	1754, 1786
FatI	CATG	7 cut(s)	334, 357, 411, 955, 1049, 1579, 1856
FauI	CCCGC	1 cut(s)	308
FauNDI	CATATG	2 cut(s)	643, 1551
FbaI	TGATCA	2 cut(s)	159, 331
FblI	GTMKAC	1 cut(s)	731
FokI	GGATG	5 cut(s)	330, 758, 854, 1097, 1526
FriOI	GRGCYC	1 cut(s)	2053
FspBI	CTAG	6 cut(s)	351, 564, 687, 1527, 1545, 1899
FspI	TGCGCA	1 cut(s)	1677
GlaI	GCGC	3 cut(s)	1524, 1677, 1855
HaeIII	GGCC	7 cut(s)	277, 362, 620, 969, 1536, 1695, 1719
HapII	CCGG	5 cut(s)	274, 363, 1278, 1413, 1696
HgaI	GACGC	3 cut(s)	280, 1740, 1742
HhaI	GCGC	3 cut(s)	1525, 1678, 1856
Hin1I	GRCGYC	2 cut(s)	291, 1734
Hin1II	CATG	7 cut(s)	338, 361, 415, 959, 1053, 1583, 1860
Hin6I	GCGC	3 cut(s)	1523, 1676, 1854
HinP1I	GCGC	3 cut(s)	1523, 1676, 1854
HincII	GTYRAC	2 cut(s)	1001, 1782
HindII	GTYRAC	2 cut(s)	1001, 1782
HpaI	GTTAAC	1 cut(s)	1782
HpaII	CCGG	5 cut(s)	274, 363, 1278, 1413, 1696
HphI	GGTGA	6 cut(s)	20, 253, 332, 515, 806, 1777
Hpy166II	GTNNAC	6 cut(s)	732, 814, 831, 1001, 1768, 1782
Hpy188I	TCNGA	6 cut(s)	164, 571, 577, 604, 1366, 1953
Hpy8I	GTNNAC	6 cut(s)	732, 814, 831, 1001, 1768, 1782
Hpy99I	CGWCG	1 cut(s)	822
HpyAV	CCTTC	9 cut(s)	220, 494, 1526, 1548, 1607, 1632, 1709, 1756, 1903
HpyCH4III	ACNGT	6 cut(s)	149, 473, 493, 818, 978, 1022
HpyCH4IV	ACGT	4 cut(s)	467, 555, 1240, 1263
HpyF3I	CTNAG	3 cut(s)	120, 1509, 1903
HpySE526I	ACGT	4 cut(s)	467, 555, 1240, 1263
Hsp92I	GRCGYC	2 cut(s)	291, 1734
Hsp92II	CATG	7 cut(s)	338, 361, 415, 959, 1053, 1583, 1860
HspAI	GCGC	3 cut(s)	1523, 1676, 1854
KroI	GCCGGC	1 cut(s)	273
KroNI	GCCGGC	1 cut(s)	275
Ksp22I	TGATCA	2 cut(s)	159, 331
KspAI	GTTAAC	1 cut(s)	1782
Kzo9I	GATC	4 cut(s)	159, 331, 962, 1647
LmnI	GCTCC	3 cut(s)	1391, 1616, 2056
Lsp1109I	GCAGC	9 cut(s)	167, 445, 800, 902, 1113, 1293, 1296, 1549, 1577
LweI	GCATC	7 cut(s)	116, 800, 1075, 1504, 1663, 1798, 1944
MaeI	CTAG	6 cut(s)	351, 564, 687, 1527, 1545, 1899
MaeII	ACGT	4 cut(s)	467, 555, 1240, 1263
MaeIII	GTNAC	6 cut(s)	8, 377, 722, 1241, 1351, 1501
MalI	GATC	4 cut(s)	161, 333, 964, 1649
MboI	GATC	4 cut(s)	159, 331, 962, 1647
MhlI	GDGCHC	4 cut(s)	498, 833, 1621, 2053
MlyI	GAGTC	3 cut(s)	239, 318, 1430
MmeI	TCCRAC	2 cut(s)	457, 1458
MnlI	CCTC	6 cut(s)	67, 158, 991, 1198, 1449, 1518
Mph1103I	ATGCAT	1 cut(s)	1813
MroNI	GCCGGC	1 cut(s)	273
MroXI	GAANNNNTTC	1 cut(s)	426
MseI	TTAA	7 cut(s)	906, 1031, 1248, 1781, 1865, 2042, 2059
MspA1I	CMGCKG	3 cut(s)	458, 1104, 2027
MspCI	CTTAAG	1 cut(s)	1030
MspI	CCGG	5 cut(s)	274, 363, 1278, 1413, 1696
MspR9I	CCNGG	3 cut(s)	143, 364, 1697
Mva1269I	GAATGC	3 cut(s)	868, 1811, 1913
MvaI	CCWGG	1 cut(s)	143
MvnI	CGCG	5 cut(s)	303, 743, 852, 1523, 1751
NaeI	GCCGGC	1 cut(s)	275
NciI	CCSGG	2 cut(s)	364, 1697
NdeI	CATATG	2 cut(s)	643, 1551
NdeII	GATC	4 cut(s)	159, 331, 962, 1647
NgoMIV	GCCGGC	1 cut(s)	273
NlaIII	CATG	7 cut(s)	338, 361, 415, 959, 1053, 1583, 1860
NlaIV	GGNNCC	3 cut(s)	78, 1393, 2032
NmeAIII	GCCGAG	1 cut(s)	635
NmuCI	GTSAC	4 cut(s)	8, 377, 722, 1241
NsbI	TGCGCA	1 cut(s)	1677
NsiI	ATGCAT	1 cut(s)	1813
NspI	RCATGY	1 cut(s)	1860
PaeR7I	CTCGAG	1 cut(s)	834
PagI	TCATGA	1 cut(s)	1579
PcsI	WCGNNNNNNNCGW	3 cut(s)	180, 552, 1527
PctI	GAATGC	3 cut(s)	868, 1811, 1913
PdiI	GCCGGC	1 cut(s)	275
PdmI	GAANNNNTTC	1 cut(s)	426
PfeI	GAWTC	8 cut(s)	258, 319, 452, 560, 572, 638, 681, 1130
PflFI	GACNNNGTC	1 cut(s)	290
PflMI	CCANNNNNTGG	1 cut(s)	412
PfoI	TCCNGGA	1 cut(s)	141
PleI	GAGTC	3 cut(s)	238, 317, 1429
PmaCI	CACGTG	1 cut(s)	1241
PmlI	CACGTG	1 cut(s)	1241
PpsI	GAGTC	3 cut(s)	238, 317, 1429
Ppu21I	YACGTR	2 cut(s)	468, 1241
PpuMI	RGGWCCY	1 cut(s)	76
Psp124BI	GAGCTC	1 cut(s)	2053
Psp1406I	AACGTT	1 cut(s)	555
Psp5II	RGGWCCY	1 cut(s)	76
Psp6I	CCWGG	1 cut(s)	141
PspCI	CACGTG	1 cut(s)	1241
PspGI	CCWGG	1 cut(s)	141
PspN4I	GGNNCC	3 cut(s)	78, 1393, 2032
PspPI	GGNCC	3 cut(s)	76, 596, 1535
PspPPI	RGGWCCY	1 cut(s)	76
PspXI	VCTCGAGB	1 cut(s)	834
PstI	CTGCAG	1 cut(s)	1567
PsyI	GACNNNGTC	1 cut(s)	290
PvuII	CAGCTG	2 cut(s)	458, 2027
RsaI	GTAC	6 cut(s)	470, 633, 840, 1168, 1654, 1889
RsaNI	GTAC	6 cut(s)	469, 632, 839, 1167, 1653, 1888
SacI	GAGCTC	1 cut(s)	2053
SaqAI	TTAA	7 cut(s)	906, 1031, 1248, 1781, 1865, 2042, 2059
Sau3AI	GATC	4 cut(s)	159, 331, 962, 1647
Sau96I	GGNCC	3 cut(s)	76, 596, 1535
ScaI	AGTACT	1 cut(s)	1654
SchI	GAGTC	3 cut(s)	239, 318, 1430
ScrFI	CCNGG	3 cut(s)	143, 364, 1697
SduI	GDGCHC	4 cut(s)	498, 833, 1621, 2053
SfaNI	GCATC	7 cut(s)	116, 800, 1075, 1504, 1663, 1798, 1944
SfcI	CTRYAG	1 cut(s)	1563
Sfr274I	CTCGAG	1 cut(s)	834
SinI	GGWCC	2 cut(s)	76, 596
SlaI	CTCGAG	1 cut(s)	834
SmlI	CTYRAG	2 cut(s)	834, 1030
SmoI	CTYRAG	2 cut(s)	834, 1030
SnaBI	TACGTA	1 cut(s)	468
SspI	AATATT	1 cut(s)	1690
SspMI	CTAG	6 cut(s)	351, 564, 687, 1527, 1545, 1899
SstI	GAGCTC	1 cut(s)	2053
StyD4I	CCNGG	3 cut(s)	141, 362, 1695
TaaI	ACNGT	6 cut(s)	149, 473, 493, 818, 978, 1022
TaiI	ACGT	4 cut(s)	470, 558, 1243, 1266
TatI	WGTACW	4 cut(s)	631, 838, 1652, 1887
TauI	GCSGC	7 cut(s)	252, 255, 280, 662, 1107, 1936, 1939
TfiI	GAWTC	8 cut(s)	258, 319, 452, 560, 572, 638, 681, 1130
Tru1I	TTAA	7 cut(s)	906, 1031, 1248, 1781, 1865, 2042, 2059
Tru9I	TTAA	7 cut(s)	906, 1031, 1248, 1781, 1865, 2042, 2059
TscAI	CASTG	2 cut(s)	242, 1294
TseFI	GTSAC	4 cut(s)	8, 377, 722, 1241
TseI	GCWGC	9 cut(s)	180, 458, 788, 915, 1101, 1281, 1284, 1562, 1565
Tsp45I	GTSAC	4 cut(s)	8, 377, 722, 1241
TspGWI	ACGGA	2 cut(s)	710, 1102
TspRI	CASTG	2 cut(s)	242, 1294
Tth111I	GACNNNGTC	1 cut(s)	290
Van91I	CCANNNNNTGG	1 cut(s)	412
Vha464I	CTTAAG	1 cut(s)	1030
VneI	GTGCAC	1 cut(s)	829
VpaK11BI	GGWCC	2 cut(s)	76, 596
XagI	CCTNNNNNAGG	1 cut(s)	1530
XapI	RAATTY	2 cut(s)	109, 690
XceI	RCATGY	1 cut(s)	1860
XhoI	CTCGAG	1 cut(s)	834
XmiI	GTMKAC	1 cut(s)	731
XmnI	GAANNNNTTC	1 cut(s)	426
XspI	CTAG	6 cut(s)	351, 564, 687, 1527, 1545, 1899
ZrmI	AGTACT	1 cut(s)	1654
Zsp2I	ATGCAT	1 cut(s)	1813

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.