Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0016338

Physical Location & Seq

Genomic Coordinates Forward (+)

64369868 .. 64372116

Gene Structure 2249 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000005017

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2085 bp

ATGACTGAGCTACCAATGGCGCGTGAAACAGACGACTCGTCCCCTCCCGCAAACAAACGCCTGAAAACCCTCAATCCCCAAATCGAATCCCAATCGGATCCAAATCAACCCGAAACCCCTCCTGAAACCGATGATTCACCGCCCGAACGCTGCGCTATTTGCTTCTTGGACGACGGCAAAGCCGCCCGAGGCAAAATCGACTGCTGCGACCACTACTTCTGCTTCTTGTGCATCAAAGAATGGGCCAAGACCGAGTCACGGTGCCCAATGTGCCGGCGGCGGTTCAACACCATCCGTCGACCGCCGAAAGTCGGCGTCTTTGTCCGTGAGCGCGTCGTTAAGGTCCCGGTTCGAGATCAGGGGAATAGAAGTGGGCATGTTGATGCTTATGAAGAAGTTGCGTGTAATGTGTGTAGCTCTATGGTAGATGATCATTTTATGTTGCTCTGTGATCTTTGCGATGGAGCGGCTCATACTTACTGTGTTGGGCTTGGTTATACTGTGCCTGAAGGTGATTGGTTTTGCAATGACTGCATTGCTTCTAGGCCTGAGCTTGCTAATGTGAATGGTGGCGATGATGAATGTGGTATGATCCCAACAGAAGCTGTGCCTGTGCCTTGTGTCTCGGTATGTGATATTGTAAGAGAATCGAATAGCTCAGCTGTTGAAAGACCTATAAGAATCACTCCAAATCCAAATCAGTGTTCGGTTGCTCCTCAAGTTGCTAGGCCAGCAAAAGCGCCACCAAGGGTGGCCAGTATATCAGCTGAATCAGCTGCAAGAACATTGGATAGGTGTCGAAATGTGCAGACTCGCATACACACACTTCGTGAGAATTGGAATGGCTTCCGGAGTGGCTCATTGAGCTTCCCTGCCAGTTCATCGAAATTGAAAGCTAGCTGCAGCAGTAGTCAAGAACATAAAAATGGTGTAATGTCTCATGAAAGATCAGGCCAAACAGAATCTTCATCTTCTCCAACTTGTCAGCAACTAGAAAATCGAGATGGTTGTGACCCCCATGATATTGACAAGGCATGGAAAATGCTGGAAAAGGCAAAGTCAAAACAGTGTATACATAAAAAGAACAGCAGTGGCGTTCAGTTGTTGCAGTTCTCGAAACTTCCTTCTAGCAGACCAAGTGCTTCTAAGGAACCAAATCCATGTCCTCATATATCAGGTCAACCACAATCCTCGTCTTTCACAGGTTGCCAGCAGCAGCAAAATCAAGTTGGTTGTGGTTCGCGTGATATTGACAAGGCATGGAAAATGGCAAAGTTGAAACAACATGTTCACGAAAACACCAGCAGTGCCCTGAAGCTTCCTTCTAGGAAAGCAAATGCTTCAGAAGAAGCAAGTAATGTGAATTCAAGTTTCGATACACTGAAAAGCCAGCAACTTGGAGCTCTGAATCAGGGAAGACCAGGAATGGAGAAGGTCTACAAAGGTAAGGAAAGGCAGTCTAGGGTCACAACTCAGGAGGCTGTAGAGTCAATCCTGGGCATTTCATGTAGTAATTCTTCACCAGGATTATTTATACCTCCATTTCCTAGGAACATAAATAGGTCAGCTTCATTACAGAAAAATCGAGCTGCACTAGGTATGGTTACTGAGCAAAACGGTTCTGCTTCTTCAATGATGGAAGGAACTTCTAATTCTTATTGTGGCAAACCTGAGCTCAGTACATCTTCTTCCGGAAACTTAGAAGAGGTCTGTGTGGAAAGCAGAGCAAGAAAAGTTGACGATGAAGCCAAAAGTGAGATCCAGTCTCTTGTCAAGATGAACTTGAAGCTTTTAAGCAAAGACCAGCGCATAGGAGTTGATGCATTTAAACAAATCGCAAGGGCTGCCACACATACCATCTTGGCTGCATGTGGTTTAGAACATCATCACAAGTCCGCCATGTATTCCTTCCCAAGCTTGGTATGCTGCCACACCGAGCAGCTTCAGCTGCCACATAAATCAAATTTGATGCGTAAGGCCTGCCGAGATTGCTTTTATGGGTTTGTGAAAGATGTTGTCAGTTCGATTCTGCAAAATCATGATGGGTTCCGACTGCGGCAGTCAAGTCACAAACAGAAATTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0006139 GO:0006351 GO:0006366 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0018130 GO:0019438 GO:0019899 GO:0019904 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0046483 GO:0070063 GO:0071704 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PHD zf-RING_2

Protein Analysis

695

Amino Acids

76.37

Weight (kDa)

8.6

Isoelectric Point (pI)

59.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	50 - 92	6.5e-07	Ring finger domain
PHD	PF00628	135 - 178	2.3e-08	PHD-finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016338)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g13310
malus_domestica	MD06G1206300.v1.1
prunus_persica	Prupe.5G210700_v2.0.a1 Prupe.5G210700_v2.0.a1
pyrus_communis	pycom06g18420
rosa_chinensis	RchiOBHm_Chr7g0183831
rosa_laevigata	RLG00000005017
rosa_multiflora	Rmu_sc0013966.1_g000004
rosa_roxburghii	Rroxscaffold_3G00270150
rosa_rugosa	Rorug06G0461100
rosa_samantha	Rh7AG061800 Rh7BG061900 Rh7CG063400 Rh7DG061600
rosa_wichuraiana	Rw7G005110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	261
AccBSI	CCGCTC	1 cut(s)	467
AccI	GTMKAC	3 cut(s)	298, 1072, 1437
AccII	CGCG	3 cut(s)	22, 333, 1243
AccIII	TCCGGA	2 cut(s)	849, 1691
AciI	CCGC	9 cut(s)	48, 140, 183, 277, 280, 302, 467, 1896, 2056
AclWI	GGATC	4 cut(s)	92, 105, 586, 1753
AcoI	YGGCCR	1 cut(s)	753
AcsI	RAATTY	2 cut(s)	1363, 1963
AcuI	CTGAAG	4 cut(s)	528, 1326, 1334, 1928
AcyI	GRCGYC	1 cut(s)	315
AdeI	CACNNNGTG	1 cut(s)	830
AfaI	GTAC	1 cut(s)	1681
AfiI	CCNNNNNNNGG	3 cut(s)	258, 311, 1918
AflIII	ACRYGT	1 cut(s)	1285
AgsI	TTSAA	7 cut(s)	286, 668, 892, 1279, 1368, 1632, 1786
AhdI	GACNNNNNGTC	1 cut(s)	37
AjnI	CCWGG	3 cut(s)	1420, 1494, 1522
AjuI	GAANNNNNNNTTGG	2 cut(s)	688, 720
Alw21I	GWGCWC	2 cut(s)	1405, 1677
Alw26I	GTCTC	3 cut(s)	628, 942, 1770
AlwI	GGATC	4 cut(s)	92, 105, 586, 1753
AlwNI	CAGNNNCTG	1 cut(s)	605
Ama87I	CYCGRG	1 cut(s)	186
Aor13HI	TCCGGA	2 cut(s)	849, 1691
AoxI	GGCC	6 cut(s)	243, 545, 728, 753, 952, 1977
ApoI	RAATTY	2 cut(s)	1363, 1963
Asp700I	GAANNNNTTC	1 cut(s)	845
AspA2I	CCTAGG	1 cut(s)	1547
AspLEI	GCGC	5 cut(s)	22, 155, 333, 742, 1809
AspS9I	GGNCC	2 cut(s)	243, 343
AsuC2I	CCSGG	1 cut(s)	347
AsuHPI	GGTGA	3 cut(s)	129, 524, 1512
AsuNHI	GCTAGC	1 cut(s)	896
AvaI	CYCGRG	1 cut(s)	186
AvaII	GGWCC	1 cut(s)	343
AvrII	CCTAGG	1 cut(s)	1547
BaeGI	GKGCMC	2 cut(s)	266, 1312
BalI	TGGCCA	1 cut(s)	755
BamHI	GGATCC	1 cut(s)	97
BanI	GGYRCC	1 cut(s)	261
BanII	GRGCYC	2 cut(s)	1405, 1677
BarI	GAAGNNNNNNTAC	4 cut(s)	1501, 1533, 1672, 1704
BbsI	GAAGAC	1 cut(s)	1423
Bbv12I	GWGCWC	2 cut(s)	1405, 1677
BccI	CCATC	6 cut(s)	299, 455, 998, 1630, 1865, 2036
BceAI	ACGGC	1 cut(s)	190
BciT130I	CCWGG	3 cut(s)	1422, 1496, 1524
BclI	TGATCA	1 cut(s)	430
BcnI	CCSGG	1 cut(s)	347
BcoDI	GTCTC	3 cut(s)	628, 942, 1770
BfaI	CTAG	9 cut(s)	543, 726, 897, 992, 1128, 1326, 1461, 1548, 1595
BfmI	CTRYAG	2 cut(s)	901, 1482
BfoI	RGCGCY	1 cut(s)	743
BlnI	CCTAGG	1 cut(s)	1547
BlpI	GCTNAGC	1 cut(s)	658
Bme1390I	CCNGG	4 cut(s)	347, 1422, 1496, 1524
Bme18I	GGWCC	1 cut(s)	343
BmeRI	GACNNNNNGTC	1 cut(s)	37
BmeT110I	CYCGRG	1 cut(s)	186
BmgT120I	GGNCC	2 cut(s)	243, 343
BmiI	GGNNCC	5 cut(s)	99, 263, 345, 1152, 2048
BmrFI	CCNGG	4 cut(s)	347, 1422, 1496, 1524
BmsI	GCATC	4 cut(s)	240, 373, 1810, 1959
BmtI	GCTAGC	1 cut(s)	900
BpiI	GAAGAC	1 cut(s)	1423
Bpu10I	CCTNAGC	2 cut(s)	549, 1671
Bpu1102I	GCTNAGC	1 cut(s)	658
BpuEI	CTTGAG	1 cut(s)	702
BpuMI	CCSGG	1 cut(s)	347
BsaBI	GATNNNNATC	1 cut(s)	102
BsaHI	GRCGYC	1 cut(s)	315
BsaJI	CCNNGG	4 cut(s)	187, 746, 1495, 1547
BsaWI	WCCGGW	2 cut(s)	849, 1691
Bsc4I	CCNNNNNNNGG	3 cut(s)	258, 311, 1918
Bse118I	RCCGGY	1 cut(s)	273
Bse1I	ACTGG	3 cut(s)	756, 876, 1762
Bse3DI	GCAATG	2 cut(s)	532, 534
Bse8I	GATNNNNATC	1 cut(s)	102
BseAI	TCCGGA	2 cut(s)	849, 1691
BseBI	CCWGG	3 cut(s)	1422, 1496, 1524
BseDI	CCNNGG	4 cut(s)	187, 746, 1495, 1547
BseGI	GGATG	1 cut(s)	291
BseJI	GATNNNNATC	1 cut(s)	102
BseLI	CCNNNNNNNGG	3 cut(s)	258, 311, 1918
BseMI	GCAATG	2 cut(s)	532, 534
BseMII	CTCAG	6 cut(s)	540, 672, 1487, 1599, 1662, 1690
BseNI	ACTGG	3 cut(s)	756, 876, 1762
BseRI	GAGGAG	1 cut(s)	705
BseSI	GKGCMC	2 cut(s)	266, 1312
BsgI	GTGCAG	2 cut(s)	827, 1575
Bsh1236I	CGCG	3 cut(s)	22, 333, 1243
Bsh1285I	CGRYCG	1 cut(s)	302
BshFI	GGCC	6 cut(s)	245, 547, 730, 755, 954, 1979
BshNI	GGYRCC	1 cut(s)	261
BsiEI	CGRYCG	1 cut(s)	302
BsiHKAI	GWGCWC	2 cut(s)	1405, 1677
BsiHKCI	CYCGRG	1 cut(s)	186
BsiSI	CCGG	4 cut(s)	274, 347, 850, 1692
BslFI	GGGAC	2 cut(s)	25, 329
BslI	CCNNNNNNNGG	3 cut(s)	258, 311, 1918
BsmAI	GTCTC	3 cut(s)	628, 942, 1770
BsmFI	GGGAC	2 cut(s)	25, 329
BsnI	GGCC	6 cut(s)	245, 547, 730, 755, 954, 1979
BsoBI	CYCGRG	1 cut(s)	186
Bsp1286I	GDGCHC	4 cut(s)	266, 1312, 1405, 1677
Bsp13I	TCCGGA	2 cut(s)	849, 1691
Bsp143I	GATC	7 cut(s)	97, 355, 430, 451, 591, 947, 1758
Bsp1720I	GCTNAGC	1 cut(s)	658
BspACI	CCGC	9 cut(s)	48, 140, 183, 277, 280, 302, 467, 1896, 2056
BspANI	GGCC	6 cut(s)	245, 547, 730, 755, 954, 1979
BspCNI	CTCAG	6 cut(s)	541, 671, 1486, 1600, 1663, 1689
BspEI	TCCGGA	2 cut(s)	849, 1691
BspFNI	CGCG	3 cut(s)	22, 333, 1243
BspHI	TCATGA	2 cut(s)	940, 2038
BspLI	GGNNCC	5 cut(s)	99, 263, 345, 1152, 2048
BspMAI	CTGCAG	1 cut(s)	905
BspOI	GCTAGC	1 cut(s)	900
BspPI	GGATC	4 cut(s)	92, 105, 586, 1753
BspT107I	GGYRCC	1 cut(s)	261
BsrBI	CCGCTC	1 cut(s)	467
BsrDI	GCAATG	2 cut(s)	532, 534
BsrFI	RCCGGY	1 cut(s)	273
BsrI	ACTGG	3 cut(s)	756, 876, 1762
BssAI	RCCGGY	1 cut(s)	273
BssECI	CCNNGG	4 cut(s)	187, 746, 1495, 1547
BssMI	GATC	7 cut(s)	97, 355, 430, 451, 591, 947, 1758
BssNAI	GTATAC	1 cut(s)	1073
BssNI	GRCGYC	1 cut(s)	315
BssT1I	CCWWGG	2 cut(s)	746, 1547
Bst1107I	GTATAC	1 cut(s)	1073
Bst2UI	CCWGG	3 cut(s)	1422, 1496, 1524
Bst4CI	ACNGT	5 cut(s)	261, 482, 502, 1068, 1619
Bst6I	CTCTTC	1 cut(s)	1698
BstACI	GRCGYC	1 cut(s)	315
BstAPI	GCANNNNNTGC	2 cut(s)	531, 1844
BstC8I	GCNNGC	7 cut(s)	275, 555, 732, 898, 1211, 1391, 1981
BstDEI	CTNAG	9 cut(s)	6, 549, 658, 1146, 1473, 1608, 1671, 1676, 1699
BstF5I	GGATG	1 cut(s)	291
BstFNI	CGCG	3 cut(s)	22, 333, 1243
BstH2I	RGCGCY	1 cut(s)	743
BstHHI	GCGC	5 cut(s)	22, 155, 333, 742, 1809
BstKTI	GATC	7 cut(s)	100, 358, 433, 454, 594, 950, 1761
BstMAI	GTCTC	3 cut(s)	628, 942, 1770
BstMBI	GATC	7 cut(s)	97, 355, 430, 451, 591, 947, 1758
BstMCI	CGRYCG	1 cut(s)	302
BstNI	CCWGG	3 cut(s)	1422, 1496, 1524
BstNSI	RCATGY	3 cut(s)	380, 1289, 1872
BstSCI	CCNGG	4 cut(s)	345, 1420, 1494, 1522
BstSFI	CTRYAG	2 cut(s)	901, 1482
BstSLI	GKGCMC	2 cut(s)	266, 1312
BstUI	CGCG	3 cut(s)	22, 333, 1243
BstV2I	GAAGAC	1 cut(s)	1423
BstX2I	RGATCY	2 cut(s)	97, 1758
BstXI	CCANNNNNNTGG	1 cut(s)	1397
BstYI	RGATCY	2 cut(s)	97, 1758
BstZ17I	GTATAC	1 cut(s)	1073
BsuRI	GGCC	6 cut(s)	245, 547, 730, 755, 954, 1979
BtgZI	GCGATG	2 cut(s)	474, 588
BtsCI	GGATG	1 cut(s)	291
BtsI	GCAGTG	2 cut(s)	1096, 1312
BtsIMutI	CAGTG	5 cut(s)	707, 1073, 1096, 1312, 1379
Cac8I	GCNNGC	7 cut(s)	275, 555, 732, 898, 1211, 1391, 1981
CaiI	CAGNNNCTG	1 cut(s)	605
CciI	TCATGA	2 cut(s)	940, 2038
CfoI	GCGC	5 cut(s)	22, 155, 333, 742, 1809
Cfr10I	RCCGGY	1 cut(s)	273
Cfr13I	GGNCC	2 cut(s)	243, 343
CseI	GACGC	2 cut(s)	304, 322
Csp6I	GTAC	1 cut(s)	1680
CviQI	GTAC	1 cut(s)	1680
DdeI	CTNAG	9 cut(s)	6, 549, 658, 1146, 1473, 1608, 1671, 1676, 1699
DpnI	GATC	7 cut(s)	99, 357, 432, 453, 593, 949, 1760
DpnII	GATC	7 cut(s)	97, 355, 430, 451, 591, 947, 1758
DraI	TTTAAA	1 cut(s)	1828
DraIII	CACNNNGTG	1 cut(s)	830
DriI	GACNNNNNGTC	1 cut(s)	37
EaeI	YGGCCR	1 cut(s)	753
Eam1104I	CTCTTC	1 cut(s)	1698
Eam1105I	GACNNNNNGTC	1 cut(s)	37
EarI	CTCTTC	1 cut(s)	1698
EciI	GGCGGA	1 cut(s)	1885
Ecl136II	GAGCTC	2 cut(s)	1403, 1675
Eco130I	CCWWGG	2 cut(s)	746, 1547
Eco147I	AGGCCT	2 cut(s)	547, 1979
Eco24I	GRGCYC	2 cut(s)	1405, 1677
Eco47I	GGWCC	1 cut(s)	343
Eco53kI	GAGCTC	2 cut(s)	1403, 1675
Eco57I	CTGAAG	4 cut(s)	528, 1326, 1334, 1928
Eco88I	CYCGRG	1 cut(s)	186
EcoICRI	GAGCTC	2 cut(s)	1403, 1675
EcoO109I	RGGNCCY	1 cut(s)	343
EcoRI	GAATTC	1 cut(s)	1363
EcoRII	CCWGG	3 cut(s)	1420, 1494, 1522
EcoT14I	CCWWGG	2 cut(s)	746, 1547
EcoT22I	ATGCAT	1 cut(s)	1825
EcoT38I	GRGCYC	2 cut(s)	1405, 1677
ErhI	CCWWGG	2 cut(s)	746, 1547
FalI	AAGNNNNNCTT	2 cut(s)	1766, 1798
FaqI	GGGAC	2 cut(s)	25, 329
FauI	CCCGC	1 cut(s)	55
FbaI	TGATCA	1 cut(s)	430
FblI	GTMKAC	3 cut(s)	298, 1072, 1437
FokI	GGATG	1 cut(s)	278
FriOI	GRGCYC	2 cut(s)	1405, 1677
FspBI	CTAG	9 cut(s)	543, 726, 897, 992, 1128, 1326, 1461, 1548, 1595
GlaI	GCGC	5 cut(s)	21, 154, 332, 741, 1808
HaeII	RGCGCY	1 cut(s)	743
HaeIII	GGCC	6 cut(s)	245, 547, 730, 755, 954, 1979
HapII	CCGG	4 cut(s)	274, 347, 850, 1692
HgaI	GACGC	2 cut(s)	304, 322
HhaI	GCGC	5 cut(s)	22, 155, 333, 742, 1809
Hin1I	GRCGYC	1 cut(s)	315
Hin6I	GCGC	5 cut(s)	20, 153, 331, 740, 1807
HinP1I	GCGC	5 cut(s)	20, 153, 331, 740, 1807
HincII	GTYRAC	3 cut(s)	299, 1181, 1738
HindII	GTYRAC	3 cut(s)	299, 1181, 1738
HindIII	AAGCTT	3 cut(s)	1316, 1787, 1915
HpaII	CCGG	4 cut(s)	274, 347, 850, 1692
HphI	GGTGA	3 cut(s)	129, 524, 1512
Hpy166II	GTNNAC	6 cut(s)	299, 1073, 1181, 1291, 1438, 1738
Hpy188I	TCNGA	4 cut(s)	97, 1345, 1407, 2051
Hpy8I	GTNNAC	6 cut(s)	299, 1073, 1181, 1291, 1438, 1738
Hpy99I	CGWCG	3 cut(s)	176, 300, 338
HpyAV	CCTTC	6 cut(s)	503, 1134, 1332, 1426, 1634, 1918
HpyCH4III	ACNGT	5 cut(s)	261, 482, 502, 1068, 1619
HpyF3I	CTNAG	9 cut(s)	6, 549, 658, 1146, 1473, 1608, 1671, 1676, 1699
Hsp92I	GRCGYC	1 cut(s)	315
HspAI	GCGC	5 cut(s)	20, 153, 331, 740, 1807
Kpn2I	TCCGGA	2 cut(s)	849, 1691
KroI	GCCGGC	1 cut(s)	273
KroNI	GCCGGC	1 cut(s)	275
Ksp22I	TGATCA	1 cut(s)	430
Kzo9I	GATC	7 cut(s)	97, 355, 430, 451, 591, 947, 1758
LmnI	GCTCC	3 cut(s)	464, 718, 1400
LweI	GCATC	4 cut(s)	240, 373, 1810, 1959
MaeI	CTAG	9 cut(s)	543, 726, 897, 992, 1128, 1326, 1461, 1548, 1595
MaeIII	GTNAC	5 cut(s)	255, 1010, 1465, 1603, 2066
MalI	GATC	7 cut(s)	99, 357, 432, 453, 593, 949, 1760
MbiI	CCGCTC	1 cut(s)	467
MboI	GATC	7 cut(s)	97, 355, 430, 451, 591, 947, 1758
MflI	RGATCY	2 cut(s)	97, 1758
MhlI	GDGCHC	4 cut(s)	266, 1312, 1405, 1677
MlsI	TGGCCA	1 cut(s)	755
MluCI	AATT	7 cut(s)	835, 887, 1363, 1513, 1651, 1963, 2078
MluNI	TGGCCA	1 cut(s)	755
MlyI	GAGTC	4 cut(s)	29, 263, 805, 1496
MmeI	TCCRAC	2 cut(s)	1001, 2074
Mox20I	TGGCCA	1 cut(s)	755
Mph1103I	ATGCAT	1 cut(s)	1825
MroI	TCCGGA	2 cut(s)	849, 1691
MroNI	GCCGGC	1 cut(s)	273
MroXI	GAANNNNTTC	1 cut(s)	845
MscI	TGGCCA	1 cut(s)	755
MseI	TTAA	3 cut(s)	339, 1793, 1827
MslI	CAYNNNNRTG	2 cut(s)	381, 924
Msp20I	TGGCCA	1 cut(s)	755
MspA1I	CMGCKG	4 cut(s)	662, 767, 776, 1948
MspI	CCGG	4 cut(s)	274, 347, 850, 1692
MspR9I	CCNGG	4 cut(s)	347, 1422, 1496, 1524
MvaI	CCWGG	3 cut(s)	1422, 1496, 1524
MvnI	CGCG	3 cut(s)	22, 333, 1243
NaeI	GCCGGC	1 cut(s)	275
NciI	CCSGG	1 cut(s)	347
NdeII	GATC	7 cut(s)	97, 355, 430, 451, 591, 947, 1758
NgoMIV	GCCGGC	1 cut(s)	273
NheI	GCTAGC	1 cut(s)	896
NlaIV	GGNNCC	5 cut(s)	99, 263, 345, 1152, 2048
NmeAIII	GCCGAG	1 cut(s)	2009
NmuCI	GTSAC	4 cut(s)	255, 1010, 1465, 2066
NsiI	ATGCAT	1 cut(s)	1825
NspI	RCATGY	3 cut(s)	380, 1289, 1872
PagI	TCATGA	2 cut(s)	940, 2038
PceI	AGGCCT	2 cut(s)	547, 1979
PciI	ACATGT	1 cut(s)	1285
PcsI	WCGNNNNNNNCGW	1 cut(s)	204
PdiI	GCCGGC	1 cut(s)	275
PdmI	GAANNNNTTC	1 cut(s)	845
PfeI	GAWTC	8 cut(s)	86, 134, 647, 681, 770, 962, 1408, 2026
PflFI	GACNNNGTC	1 cut(s)	253
PleI	GAGTC	4 cut(s)	29, 262, 805, 1495
PpsI	GAGTC	4 cut(s)	29, 262, 805, 1495
PpuMI	RGGWCCY	1 cut(s)	343
PscI	ACATGT	1 cut(s)	1285
Psp124BI	GAGCTC	2 cut(s)	1405, 1677
Psp5II	RGGWCCY	1 cut(s)	343
Psp6I	CCWGG	3 cut(s)	1420, 1494, 1522
PspGI	CCWGG	3 cut(s)	1420, 1494, 1522
PspN4I	GGNNCC	5 cut(s)	99, 263, 345, 1152, 2048
PspPI	GGNCC	2 cut(s)	243, 343
PspPPI	RGGWCCY	1 cut(s)	343
PstI	CTGCAG	1 cut(s)	905
PstNI	CAGNNNCTG	1 cut(s)	605
PsuI	RGATCY	2 cut(s)	97, 1758
PsyI	GACNNNGTC	1 cut(s)	253
PvuII	CAGCTG	4 cut(s)	662, 767, 776, 1948
RsaI	GTAC	1 cut(s)	1681
RsaNI	GTAC	1 cut(s)	1680
RseI	CAYNNNNRTG	2 cut(s)	381, 924
SacI	GAGCTC	2 cut(s)	1405, 1677
SalI	GTCGAC	1 cut(s)	297
SaqAI	TTAA	3 cut(s)	339, 1793, 1827
Sau3AI	GATC	7 cut(s)	97, 355, 430, 451, 591, 947, 1758
Sau96I	GGNCC	2 cut(s)	243, 343
SchI	GAGTC	4 cut(s)	29, 263, 805, 1496
ScrFI	CCNGG	4 cut(s)	347, 1422, 1496, 1524
SduI	GDGCHC	4 cut(s)	266, 1312, 1405, 1677
SfaNI	GCATC	4 cut(s)	240, 373, 1810, 1959
SfcI	CTRYAG	2 cut(s)	901, 1482
SinI	GGWCC	1 cut(s)	343
SmiMI	CAYNNNNRTG	2 cut(s)	381, 924
SmlI	CTYRAG	1 cut(s)	717
SmoI	CTYRAG	1 cut(s)	717
Sse9I	AATT	7 cut(s)	835, 887, 1363, 1513, 1651, 1963, 2078
SseBI	AGGCCT	2 cut(s)	547, 1979
SsiI	CCGC	9 cut(s)	48, 140, 183, 277, 280, 302, 467, 1896, 2056
SspMI	CTAG	9 cut(s)	543, 726, 897, 992, 1128, 1326, 1461, 1548, 1595
SstI	GAGCTC	2 cut(s)	1405, 1677
StuI	AGGCCT	2 cut(s)	547, 1979
StyD4I	CCNGG	4 cut(s)	345, 1420, 1494, 1522
StyI	CCWWGG	2 cut(s)	746, 1547
TaaI	ACNGT	5 cut(s)	261, 482, 502, 1068, 1619
TaqII	GACCGA	1 cut(s)	266
TasI	AATT	7 cut(s)	835, 887, 1363, 1513, 1651, 1963, 2078
TatI	WGTACW	1 cut(s)	1679
TauI	GCSGC	4 cut(s)	185, 280, 470, 2059
TfiI	GAWTC	8 cut(s)	86, 134, 647, 681, 770, 962, 1408, 2026
Tru1I	TTAA	3 cut(s)	339, 1793, 1827
Tru9I	TTAA	3 cut(s)	339, 1793, 1827
TscAI	CASTG	5 cut(s)	707, 1073, 1096, 1312, 1386
TseFI	GTSAC	4 cut(s)	255, 1010, 1465, 2066
Tsp45I	GTSAC	4 cut(s)	255, 1010, 1465, 2066
TspDTI	ATGAA	9 cut(s)	405, 594, 870, 957, 957, 1494, 1560, 1758, 1793
TspGWI	ACGGA	2 cut(s)	284, 314
TspRI	CASTG	5 cut(s)	707, 1073, 1096, 1312, 1386
Tth111I	GACNNNGTC	1 cut(s)	253
VpaK11BI	GGWCC	1 cut(s)	343
XapI	RAATTY	2 cut(s)	1363, 1963
XceI	RCATGY	3 cut(s)	380, 1289, 1872
XmaJI	CCTAGG	1 cut(s)	1547
XmiI	GTMKAC	3 cut(s)	298, 1072, 1437
XmnI	GAANNNNTTC	1 cut(s)	845
XspI	CTAG	9 cut(s)	543, 726, 897, 992, 1128, 1326, 1461, 1548, 1595
Zsp2I	ATGCAT	1 cut(s)	1825

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.